CN103797931B - One abolishes the seed dormant method of Daphne Giraldii Nitsche - Google Patents
One abolishes the seed dormant method of Daphne Giraldii Nitsche Download PDFInfo
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- CN103797931B CN103797931B CN201410064619.9A CN201410064619A CN103797931B CN 103797931 B CN103797931 B CN 103797931B CN 201410064619 A CN201410064619 A CN 201410064619A CN 103797931 B CN103797931 B CN 103797931B
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- daphne giraldii
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Abstract
The invention discloses and belong to Chinese crude drug seed quality and identify and the one of cultivation field abolishes the seed dormant method of Daphne Giraldii Nitsche.The method comprises the following steps: (1) seed disinfection: will be clean with distilled water flushing for planting experimentally son potassium permanganate solution soaking disinfection；(2) Jiao Neishizang: carry out wet Tibetan in being deposited in clamp by heap after being mixed with damp sand by the seed that step (1) obtains.The present invention abolishes the seed dormant method of Daphne Giraldii Nitsche, simple and easy to do, principle is reliable, favorable repeatability, effective, can quickly abolish the dormancy of Daphne Giraldii Nitsche seed, the germination percentage that Daphne Giraldii Nitsche seed can be effectively improved and germinating energy, work efficiency is high, reproducible, the germination percentage of Daphne Giraldii Nitsche seed reaches 30.47%, and germinating energy reaches 18.12%.
Description
Technical field
The invention belongs to Chinese crude drug seed quality identify and cultivation field, abolish Daphne Giraldii Nitsche seed particularly to one
The method of dormancy.
Technical background
Daphne Giraldii Nitsche (Daphne giraldii Nitsche) belongs to thymelaeceae daphne plant, another name knotting flower, Edgeworthia chrysantha Lindl..
Being distributed in the provinces such as China Shaanxi, Gansu, Qinghai, Henan, Gansu Province is mainly distributed on the height above sea level such as the Qilian mountains
The hillside shrubbery of 2000～2600m, border, cheuch area.The root bark of Daphne Giraldii Nitsche and peel of stem, for folk therapy
Rheumatism and the conventional Chinese medicine of pain relieving, be commonly called as Daphne giraldii Nitsche, have blood fat reducing, anti-hemolysis activity, anti-tumor activity and
The effects such as strong analgesia are it can also be used to treatment of arthritis, analgesia, antiinflammatory, surgery anesthesia etc..Plant Daphne Giraldii Nitsche mesh
Front is wild resource, is the former plant of Chinese crude drug Daphne giraldii Nitsche, and using position is root bark and peel of stem, and excessive does not conforms to
Li Liangen excavates mode, makes plant resources by heavy damage, causes the former plant of Daphne giraldii Nitsche the most in short supply；Again by
Having strong property and dormant trait in Daphne Giraldii Nitsche seed, germination percentage is the lowest, is difficult to meet Production requirement；This certainly will
Affect the breeding of Daphne Giraldii Nitsche plant and making full use of of Daphne Giraldii Nitsche plant medical value；Prior art does not have
Close the report abolishing the seed dormant method of Daphne Giraldii Nitsche, it is provided that it is to work as that one abolishes the seed dormant method of Daphne Giraldii Nitsche
It is engaged in anxious.
Summary of the invention
It is an object of the invention to provide that a kind of simple and easy to do, principle is reliable, favorable repeatability, effective abolishing
The seed dormant method of Daphne Giraldii Nitsche, this seed dormant method of Daphne Giraldii Nitsche of abolishing can quickly abolish Daphne Giraldii Nitsche seed
Dormancy, the germination percentage that Daphne Giraldii Nitsche seed can be effectively improved and germinating energy, work efficiency is high, reproducible.
Below present disclosure and step are done specific description:
The present invention abolishes the seed dormant method of Daphne Giraldii Nitsche, and described method comprises the steps,
(1) seed disinfection: will be clean with distilled water flushing for planting experimentally son potassium permanganate solution soaking disinfection；
(2) Jiao Neishizang: enter in being deposited in clamp by heap after being mixed with damp sand by the seed that step (1) obtains
The wet Tibetan of row.
During endogenous dampness Tibetan, cellar for storing things, the inhibitor contents in seed declines, seed coat softens, saturating property improves, embryo development becomes
Ripe, provide favourable conditions for seed development, acquirement better effects in seed dormancy can broken, Jiao Neishizang can
Abolish the physical property dormancy of seed efficiently, rapidly, improve the germination rate of seed, time saving and energy saving, easy to operate,
Efficiency is high.
The present invention abolishes the seed dormant method of Daphne Giraldii Nitsche, in described step (1), and described potassium permanganate solution
Mass fraction be 0.2%.
The present invention abolishes the seed dormant method of Daphne Giraldii Nitsche, in described step (1), described high for planting experimentally son
The time that potassium manganate solution soaks is 20min.
The present invention abolishes the seed dormant method of Daphne Giraldii Nitsche, and in described step (2), the humidity of described damp sand is
50%-70%, preferably 60%.
The humidity of described damp sand is to hold can agglomerating, loose can scattering be as the criterion with damp sand, and the humidity at damp sand is
During 50%-70%, described damp sand is held and can agglomerating, loose can be scattered, and in this humidity range, can promote Huang
Winter daphne seed soft and soggyization, saturating property improve, embryo development, beneficially Daphne Giraldii Nitsche are seed dormant abolishes；Damp sand
Humidity when being 60%, abolish the most most effective to Daphne Giraldii Nitsche is seed dormant, Daphne Giraldii Nitsche seed can be effectively improved
Germination percentage and germinating energy.
The present invention abolishes the seed dormant method of Daphne Giraldii Nitsche, and in described step (2), described seed is wet with described
Husky volume ratio is 1:3.
The present invention abolishes the seed dormant method of Daphne Giraldii Nitsche, and in described step (2), described seed mixes with damp sand
The stacking thickness being deposited in after conjunction in clamp is 30～50cm.
The present invention abolishes the seed dormant method of Daphne Giraldii Nitsche, and in described step (2), described seed mixes with damp sand
The stacking thickness being deposited in after conjunction in clamp is 40cm.
The present invention abolishes the seed dormant method of Daphne Giraldii Nitsche, and in described step (2), described seed mixes with damp sand
After stacking in being incorporated in clamp, on the mulling seed stacked, cover 6～16cm thick damp sands.
The present invention abolishes the seed dormant method of Daphne Giraldii Nitsche, in described step (2), described Jiao Neishizang time
Between be 20-100 days.
When Jiao Neishizang processes, along with cellar for storing things endogenous dampness hides the lengthening of time, the hard seed rate of seed is gradually lowered, and plants
Enzymatic activity within Zi improves, and enhances hydrolysis, promotes that the inhibiting substances of Interior Seed is decomposed, abolishes
The dormancy of seed；And when the cellar for storing things endogenous dampness Tibetan time is oversize, seed can be sent out owing to being in moisture state for a long time again
Mildew change, affect the germination of seed.
The present invention abolishes the seed dormant method of Daphne Giraldii Nitsche, in described step (2), described Jiao Neishizang time
Between be 80 days.
When the time of Jiao Neishizang is 80 days, both can effectively abolish the dormancy of seed, seed can be prevented again mould
Become, it is ensured that the germination percentage of seed and germinating energy.
Germinating energy refers to when germination process Sino-Japan chitting piece number reaches summit, and the seed number of germination accounts for for surveying
The percentage rate of sample seeds number, typically during initial the 1/3 of the germination test prescribed time-limit in germination number
Accounting for the percentage ratio for testing seed number is standard；Refer to that seed (is counted in the natural law that the germination test initial stage specifies for the first time
During number), normal chitting piece number accounts for the percentage ratio for planting experimentally subnumber.It is the master differentiating germination regularity
Want index.
The computing formula of germinating energy is as follows:
Germinating energy=(chitting piece number/test seed sum in alloted days) × 100%.(this test determination be
35d)
Germination percentage refer to seed in the natural law that the germination test initial stage specifies (when counting for the first time), normal chitting piece
Number accounts for the percentage ratio for planting experimentally subnumber；Refer to that test germination number accounts for the percentage ratio of test seed sum.Germination percentage
It is the leading indicator of discriminating germination regularity, is one of important indicator detecting seed quality, agricultural production
On calculate sowing quantity the most according to this.
The computing formula of germination percentage is as follows:
Germination percentage=(total grain number that germinates/test total grain number) × 100%.
The seed dormant method of Daphne Giraldii Nitsche of abolishing of the present invention have the beneficial effect that the present invention's abolishes Daphne Giraldii Nitsche kind
The method of sub-dormancy, simple and easy to do, principle is reliable, favorable repeatability, effective, can quickly abolish Daphne Giraldii Nitsche
The dormancy of seed, the germination percentage that can be effectively improved Daphne Giraldii Nitsche seed and germinating energy, work efficiency is high, reproducible.
Detailed description of the invention
Below in conjunction with embodiment, the invention will be further described:
Embodiment 1
Abolish the seed dormant method of Daphne Giraldii Nitsche, comprise the steps:
(1) seed disinfection: by for planting experimentally the potassium permanganate solution immersion 20min that son mass fraction is 0.2%
Carry out disinfection, clean with distilled water flushing；
(2) Jiao Neishizang: seed step (1) obtained carries out mixing shape with the volume ratio of 1:3 with damp sand
Being deposited in clamp endogenous dampness by heap after becoming mulling seed to hide 80 days, wherein, stacking thickness is 40cm, described
The humidity of damp sand is 60%, will cover the thick damp sand of 12cm above the mulling seed of described stacking.
Measurement result: the germination percentage of Daphne Giraldii Nitsche seed is 30.47%, germinating energy is 18.12%.
Embodiment 2
Abolish the seed dormant method of Daphne Giraldii Nitsche, comprise the steps:
(1) seed disinfection: by for planting experimentally the potassium permanganate solution immersion 20min that son mass fraction is 0.2%
Carry out disinfection, clean with distilled water flushing；
(2) Jiao Neishizang: seed step (1) obtained carries out mixing shape with the volume ratio of 1:3 with damp sand
Being deposited in clamp endogenous dampness by heap after becoming mulling seed to hide 20 days, wherein, stacking thickness is 30cm, described
The humidity of damp sand is 50%, will cover the thick damp sand of 6cm above the mulling seed of described stacking.
Measurement result: the germination percentage of Daphne Giraldii Nitsche seed is 0%, germinating energy is 0%.
Embodiment 3
Abolish the seed dormant method of Daphne Giraldii Nitsche, comprise the steps:
(1) seed disinfection: by for planting experimentally the potassium permanganate solution immersion 20min that son mass fraction is 0.2%
Carry out disinfection, clean with distilled water flushing；
(2) Jiao Neishizang: seed step (1) obtained carries out mixing shape with the volume ratio of 1:3 with damp sand
Being deposited in clamp endogenous dampness by heap after becoming mulling seed to hide 100 days, wherein, stacking thickness is 50cm, institute
The humidity stating damp sand is 60%, will cover the thick damp sand of 16cm above the mulling seed of described stacking.
Measurement result: the germination percentage of Daphne Giraldii Nitsche seed is 22.97%, germinating energy is 16.08%.
Embodiment 4
Abolish the seed dormant method of Daphne Giraldii Nitsche, comprise the steps:
(1) seed disinfection: by for planting experimentally the potassium permanganate solution immersion 20min that son mass fraction is 0.2%
Carry out disinfection, clean with distilled water flushing；
(2) Jiao Neishizang: seed step (1) obtained carries out mixing shape with the volume ratio of 1:3 with damp sand
Being deposited in clamp endogenous dampness by heap after becoming mulling seed to hide 50 days, wherein, stacking thickness is 38cm, described
The humidity of damp sand is 70%, will cover the thick damp sand of 10cm above the mulling seed of described stacking.
Measurement result: the germination percentage of Daphne Giraldii Nitsche seed is 19.40%, germinating energy is 9.30%.
Claims (6)
1. abolish the seed dormant method of Daphne Giraldii Nitsche for one kind, it is characterised in that described method is entered as follows
OK,
(1) seed disinfection: will be clean with distilled water flushing for planting experimentally son potassium permanganate solution soaking disinfection；
(2) Jiao Neishizang: seed step (1) obtained and damp sand are mixed to form after mulling seed by heap heap
It is placed in clamp, on the mulling seed stacked, covers 6～16cm thick damp sands, carry out wet Tibetan；Institute
The humidity stating damp sand is 60%；The time of described Jiao Neishizang is 80 days.
The most according to claim 1 abolish the seed dormant method of Daphne Giraldii Nitsche, it is characterised in that described
In step (1), the mass fraction of described potassium permanganate solution is 0.2%.
The most according to claim 1 abolish the seed dormant method of Daphne Giraldii Nitsche, it is characterised in that described
In step (1), the described time soaked for planting experimentally son potassium permanganate solution is 20min.
The most according to claim 1 abolish the seed dormant method of Daphne Giraldii Nitsche, it is characterised in that described
In step (2), described seed is 1:3 with the volume ratio of described wet sand.
The most according to claim 1 abolish the seed dormant method of Daphne Giraldii Nitsche, it is characterised in that described
In step (2), the stacking thickness that described seed is deposited in clamp after mixing with damp sand is 30～50cm.
The most according to claim 5 abolish the seed dormant method of Daphne Giraldii Nitsche, it is characterised in that described
In step (2), the stacking thickness that described seed is deposited in clamp after mixing with damp sand is 40cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410064619.9A CN103797931B (en) | 2014-02-25 | 2014-02-25 | One abolishes the seed dormant method of Daphne Giraldii Nitsche |
Applications Claiming Priority (1)
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|---|---|---|---|
| CN201410064619.9A CN103797931B (en) | 2014-02-25 | 2014-02-25 | One abolishes the seed dormant method of Daphne Giraldii Nitsche |
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| CN103797931A CN103797931A (en) | 2014-05-21 |
| CN103797931B true CN103797931B (en) | 2016-08-17 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104081913B (en) * | 2014-06-10 | 2015-10-28 | 甘肃凯源生物技术开发中心 | A kind of method improving D.giraldii Nitsche percentage of seedgermination |
| CN107046849A (en) * | 2017-03-28 | 2017-08-18 | 河西学院 | A kind of efficient germination accelerating method of D.giraldii Nitsche seed |
| CN107018704A (en) * | 2017-03-30 | 2017-08-08 | 河西学院 | A kind of method of raising Tang Gute winter daphne percentages of seedgermination |
| CN108633375A (en) * | 2018-04-04 | 2018-10-12 | 赵崇来 | A method of abolishing wild D.giraldii Nitsche seed dormancy |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009232755A (en) * | 2008-03-27 | 2009-10-15 | National Agriculture & Food Research Organization | Treatment method for improving germination |
| CN102845199A (en) * | 2012-09-03 | 2013-01-02 | 甘肃泰康制药有限责任公司 | Artificial seed breeding technology of wild girald daphne bark |
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2014
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009232755A (en) * | 2008-03-27 | 2009-10-15 | National Agriculture & Food Research Organization | Treatment method for improving germination |
| CN102845199A (en) * | 2012-09-03 | 2013-01-02 | 甘肃泰康制药有限责任公司 | Artificial seed breeding technology of wild girald daphne bark |
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| CN103797931A (en) | 2014-05-21 |
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Granted publication date: 20160817 Termination date: 20190225 |