CN107046849A - A kind of efficient germination accelerating method of D.giraldii Nitsche seed - Google Patents
A kind of efficient germination accelerating method of D.giraldii Nitsche seed Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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Abstract
The invention discloses a kind of efficient germination accelerating method of D.giraldii Nitsche seed, comprise the following steps：Harvesting ripe, in D.giraldii Nitsche fruit during peony, is washed by rubbing with the hands and dried in the shade in removal pericarp and pulp rear chamber to pericarp, is dried in the shade shared 3 days to interior from harvesting；Select full seed be placed in it is standby in kraft paper bag；15 days are stood in 25 DEG C of insulating boxs；15 days are stood in 30 DEG C of insulating boxs；Solution is changed with 150mgL 1 GA3 solution seed soakings 24h, 8h once；Then it is clean and interior is dried in the shade for 12 hours with distilled water flushing；It is then clean with distilled water flushing with 5% liquor potassic permanganate soaking disinfection 10 minutes；River sand and treated seed are taken by 1：5 ratio is mixed in germination box, is put in the refrigerator for being placed in 7 DEG C 25 days.Simple the beneficial effects of the invention are as follows, process step, easily operation, repeatability is high, convenient to promote, and the equipment and reagent are common, cheap, it is often more important that abolish the efficiency high of seed dormancy, it is necessary to time it is short.
Description
Technical field
The invention belongs to vegetable seeds vernalization field, and in particular to a kind of efficient germination accelerating method of D.giraldii Nitsche seed.
Background technology
D.giraldii Nitsche (Daphne giraldii Nitsche.), belongs to Thymelaeceae, daphne plant, alias knotting flower, Edgeworthia chrysantha.
Wild D.giraldii Nitsche is distributed mainly on the provinces and regions such as Shaanxi, Gansu, Sichuan, Qinghai, Ningxia, and the provinces and regions such as Henan, Jiangxi and Shanxi also have
Distribution, is typically grown on height above sea level 1600m or so mountain region.Gansu Province mainly divides stem skin and root skin to be used as medicine, title girald daphne bark, pungent, hardship,
It is warm-natured, it is slightly poisonous, the effect of with dispelling wind and removing obstruction in the meridians, stasis-dispelling and pain-killing, it is clinically used for traumatic injury, rheumatic arthritis and analgesic
Deng, be folk therapy rheumatism and analgesic conventional Chinese medicine.Preparation have Zushima gaoyao, girald daphne bark's analgesic rheumatoid plaster, Giraldi daphne tablet,
Daphne giraldii Nitsche injection liquid.
The dormancy of seed is plant for environmental factors such as acclimatization change, temperature difference, moisture conditions and kept
A kind of biological characteristics formed by self-reproduction development, has great importance.Thus many more cold districts grow
Vegetable seeds is difficult to sprout after fruit maturation under suitable temperature, humidity and illumination condition in autumn, keeps resting state straight
Just sprouted to next spring.The wild D.giraldii Nitsche in the Qilian mountains is grown in the gentle slope area of half shady half light of 2500 meters or so of hypo,
It is the association undershrub of the arbors such as dragon spruce.In order to adapt to the Qilian mountains cold winter and nice and cool summer climate, D.giraldii Nitsche has
Stronger tolerance to cold and its seed has hibernation feature.From D.giraldii Nitsche fruit maturation in July, until its pulp of mid or late September
Seed is wrapped in always.
Plant D.giraldii Nitsche is at present wild resource, is the former plant of Chinese medicine girald daphne bark, the use of position is root skin and stem skin,
Excessive unreasonable even root excavation mode, makes plant resources by heavy damage, causes the former plant of girald daphne bark extremely in short supply.Due to
Wild D.giraldii Nitsche seed has significant dormant trait, and germination percentage is very low, it is difficult to meet production requirement, this certainly will influence wild Huang
The artificial propagation of winter daphne and making full use of for its medical value.CN102845199A discloses a kind of wild girald daphne bark's seed growing
Technology, it is colonized by seed collection, earthing of seeds, nursery, field-transplanting, field manages and protects these steps and completes seed growing.
Girald daphne bark is bred using above-mentioned steps, can make girald daphne bark's percentage of seedgermination is stable to reach after more than 71.3%, Stratificated treatment
Seed planting percent is up to more than 66%, and featured rear planting percent is up to more than 85%.Wild girald daphne bark's seed growing technical research into
Work(, indicates that wild girald daphne bark enters girald daphne bark's artificial seed raising technology, and it is planted by seed collection, seed row artificial breeding
It can realize completely.CN103797931A, which is disclosed, belongs to the identification of Chinese medicine seed quality and one kind of cultivation field abolishes Huang
The method of winter daphne seed dormancy.This method comprises the following steps：(1) seed disinfection：It will be soaked for planting experimentally sub- liquor potassic permanganate
Bubble sterilization, it is clean with distilled water flushing；(2) wet storage in storing：The seed that step (1) is obtained is deposited in after being mixed with damp sand by heap
Wet storage is carried out in clamp.The method of the efficient vernalization of D.giraldii Nitsche seed of the present invention, simple and easy to do, principle is reliable, favorable repeatability,
Effect is good, can quickly abolish the dormancy of D.giraldii Nitsche seed, can effectively improve the germination percentage and germinating energy of D.giraldii Nitsche seed, work effect
Rate is high, reproducible, and the germination percentage of D.giraldii Nitsche seed reaches 30.47%, and germinating energy reaches 18.12%.A kind of quick solution is provided
Except the method for wild D.giraldii Nitsche seed dormancy is the task of top priority of artificial quick, large area nursery.
The content of the invention
In view of the above-mentioned problems, the present invention proposes a kind of efficient germination accelerating method of D.giraldii Nitsche seed, it is specific as follows：
A kind of efficient germination accelerating method of D.giraldii Nitsche seed, at least stands step including 25 DEG C first carried out successively and 30 DEG C of constant temperature
Suddenly, the GA3 solution seed soaking steps and afterwards carried out.
It is preferred that, 25 DEG C carried out successively and 30 DEG C of constant temperature process steps are：First 5-30 is stood in 25 DEG C of insulating boxs
My god, then stand 5-30 days in 30 DEG C of insulating boxs；
It is preferred that, the GA3 solution seed soakings step is：Change molten with 10-500mgL-1GA3 solution seed soakings 12h-48h, 8h
Liquid is once；Then with distilled water flushing is clean and interior is dried in the shade 12h；
It is further preferred that 25 DEG C carried out successively and 30 DEG C of constant temperature process steps are：It is first quiet in 25 DEG C of insulating boxs
Put 15 days, then 15 days are stood in 30 DEG C of insulating boxs；
It is further preferred that the GA3 solution seed soakings step is：Change molten with 150mgL-1GA3 solution seed soakings 24h, 8h
Liquid is once；Then it is clean and interior is dried in the shade for 12 hours with distilled water flushing；
It is preferred that, in addition to 25 DEG C carried out successively and 30 DEG C of constant temperature are the step of stand before steps and GA3 solution seed soakings
The step of after step；25 DEG C carried out successively and 30 DEG C of constant temperature stand steps before the step of be：Harvesting ripe is to really
D.giraldii Nitsche fruit when skin is in peony, is washed by rubbing with the hands and is dried in the shade in removal pericarp and pulp rear chamber, and shared 3 are dried in the shade from harvesting to interior
My god；Select full seed be placed in it is standby in kraft paper bag；The step of after the GA3 solution seed soakings step is：First, take GA3 molten
The seed that immersion kind step process is crossed, it is then dry with distilled water flushing with 5% liquor potassic permanganate soaking disinfection 10 minutes
Only；Again, river sand and liquor potassic permanganate soaking disinfection and the treated seed of distilled water flushing are taken by 5:1 ratio is mixed in hair
In bud box, put in the refrigerator for being placed in 7 DEG C 25 days.
After harvesting, removal pericarp and the pulp of the present invention, dry in the shade, select process step, it is therefore intended that selecting preparation follow-up
The seed of processing；Single 25 DEG C and 30 DEG C of constant temperature process steps, it is therefore intended that promote physiology after-ripening；Single GA3 solution leaching
Plant process step, it is therefore intended that shorten dormancy time；Liquor potassic permanganate immersion treatment step, it is therefore intended that go to sterilize, suppress
The microorganism of the surface of the seed；Refrigerator process step is put in river sand and seed mixing, and purpose promotes kind of a skin broken shell, budding.
Heretofore described GA3 is gibberellin, and 5% described liquor potassic permanganate is mass volume ratio, described
River sand is wet river sand, and holding agglomerating, loose can scatter.
The inventive method makes seed complete physiology after-ripening by constant temperature stewing process seed, so as to be conducive to beating for dormancy
It is broken；What further gibberellin seed soaking and the combination of cool stratification can significantly shorten its dormancy breaks the time, from collecting seed kind
Sub- termination of diapause, seed breach, the breach rate for just having reached 92% in front and rear 58 days or so.
The beneficial effects of the invention are as follows the present invention proposes a kind of efficient germination accelerating method of D.giraldii Nitsche seed, process step
Simply, easily operation, repeatability is high, convenient to promote, and the equipment and reagent are common, cheap, it is often more important that to abolish
The efficiency high of seed dormancy, it is necessary to time it is short.
Embodiment
To describe technology contents, construction feature, institute's reached purpose and effect of the present invention in detail, embodiment is hereby enumerated below
It is explained in detail.
Embodiment 1
A kind of efficient germination accelerating method of D.giraldii Nitsche seed, comprises the following steps：
S1, D.giraldii Nitsche fruit when harvesting ripe to pericarp is in peony is washed by rubbing with the hands and dried in the shade in removal pericarp and pulp rear chamber,
Dried in the shade shared 3 days to interior from harvesting；Select full seed be placed in it is standby in kraft paper bag；
5 days are stood in S2, the seed for taking S1 step process to cross, 25 DEG C of insulating boxs；
5 days are stood in S3, the seed for taking S2 step process to cross, 30 DEG C of insulating boxs；
S4, the seed for taking S3 step process to cross, uses 10mgL-1GA3 solution seed soakings 12h, 8h change solution once；Then use
Distilled water flushing is clean and interior is dried in the shade for 12 hours；
S5, the seed for taking S4 step process to cross, with 5% liquor potassic permanganate soaking disinfection 10 minutes, then with distillation
Water is rinsed well；
S6, takes the seed that river sand and S5 step process are crossed by 5:1 ratio is mixed in germination box, is placed in 7 DEG C of refrigerator
In put 25 days.
Embodiment 2
A kind of efficient germination accelerating method of D.giraldii Nitsche seed, comprises the following steps：
S1, D.giraldii Nitsche fruit when harvesting ripe to pericarp is in peony is washed by rubbing with the hands and dried in the shade in removal pericarp and pulp rear chamber,
Dried in the shade shared 3 days to interior from harvesting；Select full seed be placed in it is standby in kraft paper bag；
5 days are stood in S2, the seed for taking S1 step process to cross, 25 DEG C of insulating boxs；
5 days are stood in S3, the seed for taking S2 step process to cross, 30 DEG C of insulating boxs；
S4, the seed for taking S3 step process to cross, uses 500mgL-1GA3 solution seed soakings 48h, 8h change solution once；Then use
Distilled water flushing is clean and interior is dried in the shade for 12 hours；
S5, the seed for taking S4 step process to cross, with 5% liquor potassic permanganate soaking disinfection 10 minutes, then with distillation
Water is rinsed well；
S6, takes the seed that river sand and S5 step process are crossed by 5:1 ratio is mixed in germination box, is placed in 7 DEG C of refrigerator
In put 25 days.
Embodiment 3
A kind of efficient germination accelerating method of D.giraldii Nitsche seed, comprises the following steps：
S1, D.giraldii Nitsche fruit when harvesting ripe to pericarp is in peony is washed by rubbing with the hands and dried in the shade in removal pericarp and pulp rear chamber,
Dried in the shade shared 3 days to interior from harvesting；Select full seed be placed in it is standby in kraft paper bag；
30 days are stood in S2, the seed for taking S1 step process to cross, 25 DEG C of insulating boxs；
30 days are stood in S3, the seed for taking S2 step process to cross, 30 DEG C of insulating boxs；
S4, the seed for taking S3 step process to cross, uses 10mgL-1GA3 solution seed soakings 12h, 8h change solution once；Then use
Distilled water flushing is clean and interior is dried in the shade for 12 hours；
S5, the seed for taking S4 step process to cross, with 5% liquor potassic permanganate soaking disinfection 10 minutes, then with distillation
Water is rinsed well；
S6, takes the seed that river sand and S5 step process are crossed by 5:1 ratio is mixed in germination box, is placed in 7 DEG C of refrigerator
In put 25 days.
Embodiment 4
A kind of efficient germination accelerating method of D.giraldii Nitsche seed, comprises the following steps：
S1, D.giraldii Nitsche fruit when harvesting ripe to pericarp is in peony is washed by rubbing with the hands and dried in the shade in removal pericarp and pulp rear chamber,
Dried in the shade shared 3 days to interior from harvesting；Select full seed be placed in it is standby in kraft paper bag；
30 days are stood in S2, the seed for taking S1 step process to cross, 25 DEG C of insulating boxs；
30 days are stood in S3, the seed for taking S2 step process to cross, 30 DEG C of insulating boxs；
S4, the seed for taking S3 step process to cross, uses 500mgL-1GA3 solution seed soakings 48h, 8h change solution once；Then use
Distilled water flushing is clean and interior is dried in the shade for 12 hours；
S5, the seed for taking S4 step process to cross, with 5% liquor potassic permanganate soaking disinfection 10 minutes, then with distillation
Water is rinsed well；
S6, takes the seed that river sand and S5 step process are crossed by 5:1 ratio is mixed in germination box, is placed in 7 DEG C of refrigerator
In put 25 days.
Embodiment 5
A kind of efficient germination accelerating method of D.giraldii Nitsche seed, comprises the following steps：
S1, D.giraldii Nitsche fruit when harvesting ripe to pericarp is in peony is washed by rubbing with the hands and dried in the shade in removal pericarp and pulp rear chamber,
Dried in the shade shared 3 days to interior from harvesting；Select full seed be placed in it is standby in kraft paper bag；
15 days are stood in S2, the seed for taking S1 step process to cross, 25 DEG C of insulating boxs；
15 days are stood in S3, the seed for taking S2 step process to cross, 30 DEG C of insulating boxs；
S4, the seed for taking S3 step process to cross, uses 150mgL-1GA3 solution seed soakings 24h, 8h change solution once；Then use
Distilled water flushing is clean and interior is dried in the shade for 12 hours；
S5, the seed for taking S4 step process to cross, with 5% liquor potassic permanganate soaking disinfection 10 minutes, then with distillation
Water is rinsed well；
S6, takes the seed that river sand and S5 step process are crossed by 5:1 ratio is mixed in germination box, is placed in 7 DEG C of refrigerator
In put 25 days.
Reference examples 1
A kind of efficient germination accelerating method of D.giraldii Nitsche seed, comprises the following steps：
S1, D.giraldii Nitsche fruit when harvesting ripe to pericarp is in peony is washed by rubbing with the hands and dried in the shade in removal pericarp and pulp rear chamber,
Dried in the shade shared 3 days to interior from harvesting；Select full seed be placed in it is standby in kraft paper bag；
S5, the seed for taking S1 step process to cross, with 5% liquor potassic permanganate soaking disinfection 10 minutes, then with distillation
Water is rinsed well；
S6, takes the seed that river sand and S5 step process are crossed by 5:1 ratio is mixed in germination box, is placed in 7 DEG C of refrigerator
In put 25 days.
Reference examples 2
A kind of efficient germination accelerating method of D.giraldii Nitsche seed, comprises the following steps：
S1, D.giraldii Nitsche fruit when harvesting ripe to pericarp is in peony is washed by rubbing with the hands and dried in the shade in removal pericarp and pulp rear chamber,
Dried in the shade shared 3 days to interior from harvesting；Select full seed be placed in it is standby in kraft paper bag；
S4, the seed for taking S1 step process to cross, uses 150mgL-1GA3 solution seed soakings 24h, 8h change solution once；Then use
Distilled water flushing is clean and interior is dried in the shade for 12 hours；
S5, the seed for taking S4 step process to cross, with 5% liquor potassic permanganate soaking disinfection 10 minutes, then with distillation
Water is rinsed well；
S6, takes the seed that river sand and S5 step process are crossed by 5:1 ratio is mixed in germination box, is placed in 7 DEG C of refrigerator
In put 25 days.
Reference examples 3
A kind of efficient germination accelerating method of D.giraldii Nitsche seed, comprises the following steps：
S1, D.giraldii Nitsche fruit when harvesting ripe to pericarp is in peony is washed by rubbing with the hands and dried in the shade in removal pericarp and pulp rear chamber,
Dried in the shade shared 3 days to interior from harvesting；Select full seed be placed in it is standby in kraft paper bag；
15 days are stood in S2, the seed for taking S1 step process to cross, 25 DEG C of insulating boxs；
15 days are stood in S3, the seed for taking S2 step process to cross, 30 DEG C of insulating boxs；
S5, the seed for taking S3 step process to cross, with 5% liquor potassic permanganate soaking disinfection 10 minutes, then with distillation
Water is rinsed well；
S6, takes the seed that river sand and S5 step process are crossed by 5:1 ratio is mixed in germination box, is placed in 7 DEG C of refrigerator
In put 25 days.
Reference examples 4
A kind of efficient germination accelerating method of D.giraldii Nitsche seed, comprises the following steps：
S1, D.giraldii Nitsche fruit when harvesting ripe to pericarp is in peony is washed by rubbing with the hands and dried in the shade in removal pericarp and pulp rear chamber,
Dried in the shade shared 3 days to interior from harvesting；Select full seed be placed in it is standby in kraft paper bag；
15 days are stood in S3, the seed for taking S2 step process to cross, 30 DEG C of insulating boxs；
S4, the seed for taking S1 step process to cross, uses 150mgL-1GA3 solution seed soakings 24h, 8h change solution once；Then use
Distilled water flushing is clean and interior is dried in the shade for 12 hours；
S5, the seed for taking S3 step process to cross, with 5% liquor potassic permanganate soaking disinfection 10 minutes, then with distillation
Water is rinsed well；
S6, takes the seed that river sand and S5 step process are crossed by 5:1 ratio is mixed in germination box, is placed in 7 DEG C of refrigerator
In put 25 days.
Reference examples 5
A kind of efficient germination accelerating method of D.giraldii Nitsche seed, comprises the following steps：
S1, D.giraldii Nitsche fruit when harvesting ripe to pericarp is in peony is washed by rubbing with the hands and dried in the shade in removal pericarp and pulp rear chamber,
Dried in the shade shared 3 days to interior from harvesting；Select full seed be placed in it is standby in kraft paper bag；
15 days are stood in S3, the seed for taking S2 step process to cross, 30 DEG C of insulating boxs；
S4, the seed for taking S1 step process to cross, uses 150mgL-1GA3 solution seed soakings 24h, 8h change solution once；Then use
Distilled water flushing is clean and interior is dried in the shade for 12 hours；
S5, the seed for taking S3 step process to cross, with 5% liquor potassic permanganate soaking disinfection 10 minutes, then with distillation
Water is rinsed well；
S6, takes the seed that river sand and S5 step process are crossed by 5:1 ratio is mixed in germination box, is placed in 7 DEG C of refrigerator
In put 25 days.
Influence of the processing method of experimental example 1 to wild D.giraldii Nitsche seed breach rate
Effect of the processing method to wild D.giraldii Nitsche presprouting of seeds, the calculating of breach rate are evaluated in this experiment using breach rate
Formula is：Breach rate=(breach seed number/total seed number) × 100%.A kind of efficient germination accelerating method of D.giraldii Nitsche seed
Adopted for D.giraldii Nitsche (Daphne giraldii Nitsche.) seed of examination in from the sweet of height above sea level 2500m~2600m
Su Sheng Wuweis Tianzhu Qilian township boolean grassland (103 ° 33 ' 26 " E, 38 ° 43 ' 31 " N), slope aspect thing, the gradient is about
25°.Fruit maturation is plucked when being in peony to pericarp.
The pericarp for taking the identical maturity with one tree is in wine-colored fruit, after embodiment 1-5 processing, is being placed in
The 25th day observation result in 7 DEG C of refrigerator.Seed is taken out from river sand, observation kind skin whether there is breach, calculates breach rate.Processing
Total number of days is designated as 0.5 day, was designated as less than 1 hour 0 day in units of day for 12 hours.After reference examples 1-5 processing, 7 DEG C are being placed in
The 25th day observation result in refrigerator, is then proceeded to until total number of days observes result on the 59.5th day again.
The breach rate of the wild D.giraldii Nitsche seed of the different disposal method of table 1
* number of symbols is identical represents no significant difference, and number difference indicates significant difference (P=0.05)
From table 1 it follows that reference examples 1-3 first time and second observation result, wild D.giraldii Nitsche seed splits
Mouthful rate is 0, illustrate single constant temperature stand and GA3 solution seed soakings can not effective Dormancy breaking, reference examples 4-5 first time with
Second observation result breach rate is also 0, illustrate the constant temperature standing of 25 ° and 30 ° two single temperature respectively with GA3 solution seed soakings
Combined Treatment can not effectively abolish wild D.giraldii Nitsche seed dormancy.
The breach rate of embodiment 1-5 wild D.giraldii Nitsche seed can reach more than 40%, significantly larger than reference examples 1-5's
Two observation results, illustrates that the constant temperature standing of successively 25 ° and 30 ° two single temperature and GA3 solution seed soakings Combined Treatment can be with
The dormancy of wild D.giraldii Nitsche seed is effectively abolished, 25 ° of constant temperature are stood, 30 ° of constant temperature are stood and three process steps of GA3 solution seed soakings
Between there is concertedness, and 25 ° of constant temperature stand and stood and two processing of GA3 solution seed soakings with GA3 solution seed soakings and 30 ° of constant temperature
Do not have concertedness between step.Embodiment 2 compared with Example 3, increases GA3 solution seed soakings with embodiment 1, embodiment 4
Concentration and the time (from 10mgL-1Soak seed 12h to 500mgL-1Soak seed 48h), breach rate is variant but does not have conspicuousness,
It is not linear between the concentration of GA3 solution seed soakings and time and breach rate.Embodiment 3 and embodiment 1, embodiment 4 and reality
Apply example 2 to compare, time of 25 ° of increase and 30 ° of constant temperature standing (from being 5 days to being 30), breach rate is variant but does not have aobvious
Work property, it is also not linear between the time of constant temperature standing and breach rate.
D.giraldii Nitsche seed breach rate is processed as optimal with embodiment 5 in embodiment 1-5, is significantly higher than embodiment 1-4, unexpectedly
92% has been reached, has illustrated to stand 15 days in standing 15 days, 30 DEG C of insulating boxs in 25 DEG C of insulating boxs and uses 150mgL-1GA3 solution
Seed soaking 24h can fast and effectively abolish seed dormancy.Total number of days is handled simultaneously for 58 days or so, and the total number of days of processing is shorter,
It can be used in the artificial quick, actual production of large area nursery.
The inventive method makes seed complete physiology after-ripening by constant temperature stewing process seed, so as to be conducive to beating for dormancy
It is broken；Further gibberellin seed soaking can significantly shorten breaking the time for its dormancy, two constant temperature stewing process steps and red mould
There is associated collaborative effect between element seed soaking.
In summary, only the preferred embodiments of the invention, does not limit protection scope of the present invention with this, all according to the present invention
The equivalent changes and modifications that the scope of the claims and description are made, is all within the scope of patent of the present invention covers.
Claims (6)
1. a kind of efficient germination accelerating method of D.giraldii Nitsche seed, it is characterised in that at least including first 25 DEG C and 30 DEG C carried out successively
Constant temperature stands step, and the GA3 solution seed soaking steps carried out afterwards.
2. the efficient germination accelerating method of D.giraldii Nitsche seed as claimed in claim 1, it is characterised in that 25 DEG C carried out successively
It is with 30 DEG C of constant temperature process steps：
First stood in 25 DEG C of insulating boxs in 5-30 days, then 30 DEG C of insulating boxs and stand 5-30 days.
3. the efficient germination accelerating method of D.giraldii Nitsche seed as claimed in claim 1, it is characterised in that the GA3 solution seed soakings step
Suddenly it is：
Use 10-500mgL-1GA3 solution seed soakings 12h-48h, 8h change solution once；Then it is clean and indoor with distilled water flushing
Dry in the shade 12h.
4. the efficient germination accelerating method of D.giraldii Nitsche seed as claimed in claim 2, it is characterised in that 25 DEG C carried out successively
It is with 30 DEG C of constant temperature process steps：
First stood in 25 DEG C of insulating boxs in 15 days, then 30 DEG C of insulating boxs and stand 15 days.
5. the efficient germination accelerating method of D.giraldii Nitsche seed as claimed in claim 3, it is characterised in that the GA3 solution seed soakings step
Suddenly it is：
Use 150mgL-1GA3 solution seed soakings 24h, 8h change solution once；Then with distilled water flushing is clean and indoor 12 hours the moon
It is dry.
6. the efficient germination accelerating method of the D.giraldii Nitsche seed as described in claim 1-6 is any, it is characterised in that also including entering successively
The step of after the step of capable 25 DEG C and 30 DEG C of constant temperature are stood before steps and GA3 solution seed soaking steps；
25 DEG C carried out successively and 30 DEG C of constant temperature stand steps before the step of be：
Harvesting ripe, in D.giraldii Nitsche fruit during peony, is washed by rubbing with the hands and dried in the shade in removal pericarp and pulp rear chamber, from harvesting to pericarp
Dried in the shade shared 3 days to interior；Select full seed be placed in it is standby in kraft paper bag；
The step of after the GA3 solution seed soakings step is：
First, the seed for taking GA3 solution seed soaking step process to cross, with 5% liquor potassic permanganate soaking disinfection 10 minutes, Ran Houyong
Distilled water flushing is clean；
Again, river sand and liquor potassic permanganate soaking disinfection and the treated seed of distilled water flushing are taken by 5:1 ratio mix in
Germinate in box, put in the refrigerator for being placed in 7 DEG C 25 days.
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| CN201710193311.8A CN107046849A (en) | 2017-03-28 | 2017-03-28 | A kind of efficient germination accelerating method of D.giraldii Nitsche seed |
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| CN201710193311.8A CN107046849A (en) | 2017-03-28 | 2017-03-28 | A kind of efficient germination accelerating method of D.giraldii Nitsche seed |
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| CN201710193311.8A Pending CN107046849A (en) | 2017-03-28 | 2017-03-28 | A kind of efficient germination accelerating method of D.giraldii Nitsche seed |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109691266A (en) * | 2019-02-02 | 2019-04-30 | 中国医学科学院药用植物研究所 | A kind of method and its application of Constant Temperature Detection cork tree seed dormancy degree |
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| CN103797931A (en) * | 2014-02-25 | 2014-05-21 | 甘肃凯源生物技术开发中心 | Method for breaking dormancy of daphne giraldii seeds |
| CN103797932A (en) * | 2014-02-25 | 2014-05-21 | 甘肃凯源生物技术开发中心 | Hard seed breaking device for promoting daphne giraldii seeds to germinate |
| CN104081913A (en) * | 2014-06-10 | 2014-10-08 | 甘肃凯源生物技术开发中心 | Method for increasing germination rate of daphne giraldii nitsche seeds |
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| CN103797931A (en) * | 2014-02-25 | 2014-05-21 | 甘肃凯源生物技术开发中心 | Method for breaking dormancy of daphne giraldii seeds |
| CN103797932A (en) * | 2014-02-25 | 2014-05-21 | 甘肃凯源生物技术开发中心 | Hard seed breaking device for promoting daphne giraldii seeds to germinate |
| CN104081913A (en) * | 2014-06-10 | 2014-10-08 | 甘肃凯源生物技术开发中心 | Method for increasing germination rate of daphne giraldii nitsche seeds |
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| CN109691266A (en) * | 2019-02-02 | 2019-04-30 | 中国医学科学院药用植物研究所 | A kind of method and its application of Constant Temperature Detection cork tree seed dormancy degree |
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