CN101199252A - Leymus chinensis high-temperature seed presoaking and temperature changing combination germinating method - Google Patents
Leymus chinensis high-temperature seed presoaking and temperature changing combination germinating method Download PDFInfo
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- CN101199252A CN101199252A CNA2006101632466A CN200610163246A CN101199252A CN 101199252 A CN101199252 A CN 101199252A CN A2006101632466 A CNA2006101632466 A CN A2006101632466A CN 200610163246 A CN200610163246 A CN 200610163246A CN 101199252 A CN101199252 A CN 101199252A
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Abstract
The invention is a chinensis germination method of combined high temperature seed soaking and temperature changing, which belongs to the planting technique field. The invention mainly comprises two parts of partially releasing the chinensis seed dormancy by utilizing the seed soaking method in the seed pretreatment technique and rapidly increasing the seed germination rate of chinensis by applying the variable temperature technique. The invention concretely has four processes of 1) seed selection and impurity removal, 2) high temperature seed soaking, 3) seed disinfection and 4) variable temperature germination. The invention can increase the chinensis germination rate from 56.7 percent to above 84.0 percent compared with the chinensis seed treatment by a single method. Therefore, the invention has significant practical meaning and broad application value to both the chinensis seed cultivation and experimental study.
Description
Technical field
The invention belongs to the plant planting technical field, relate to the germinating method of plant seed breaking dormancy, specifically break the severe dormancy of Leymus chinensis seeds fast, significantly improve the combined method of Leymus chinensis seeds germination rate.
Background technology
Sheep's hay (Leymus chinensis) is the perennial rhizome plant of grass family leymus, is a kind of high quality forage.This herbage mainly is distributed in the eastern region on Eurasian prairie, comprises Muscovite outer Baikal, the east of Mongolia and the north, northeast song-Nen plain, the North China Plain and the Nei Mongol Plateau area of China.Sheep's hay has the very high economic worth and the ecological value, is rich in high-quality protein, mineral matter and carbohydrate.Its fast growth, drought-resistant saline and alkaline and dead soil.Yet, in recent years because the overgraze and the reclamation of wasteland cause original a large amount of high-quality sheep's hays meadow to become the saline and alkaline desert that has no value.Therefore, how to improve degenerated soil, enlarge the task of top priority (Liang Zhengwei etc., 2004, the method for soda alkaline land sheep's hay vegetation restoration and reconstruction, number of patent application 200410010867.1) that the sheep's hay cultivated area has become production and scientific research fast.
Leymus chinensis seeds has the unique biological characteristic, and resting stage reaches more than the 4-5.New seed generally only has the low germination rate of 10-20%.One of main bottleneck technology that present plantation sheep's hay faces is severe dormancy how to remove Leymus chinensis seeds fast, significantly improves the problem of percentage of seedgermination.Former studies shows that Leymus chinensis seeds germination rate under 25-26 ℃ constant temperature is very low.Germination rate when not adding any medicine and handle only is 1.8%; Use 1%HNO
3The germination rate of handling is 8%.In addition, in the soil germinating bed of differing texture, chernozem germination rate the highest (42.8%), clay take second place (27.9%), and sand is 20.2%.Yi Jin (dormant physiology of Leymus chinensis seeds and the research that improves germination rate, the China meadow, 1994, (6): 1-6) Leymus chinensis seeds after will gathering in the crops places dry storage under the indoor 15-25 ℃ normal temperature, carrying out germination rate by the seed to storage 1-8 at 20 ℃ of constant temperatures measures, find paper training method to preserve the minimum of 1 year and 8 years, germination rate only is 1%; Preserve the highest of 4 years and 5 years, but germination rate also has only 5%; Sha Peifa is to preserve 1 year minimum, and germination rate is 7%, and to preserve 6 years the highest, germination rate is 45%; The earth culture method with preserve 1 year be 36%, the germination rate of preserving 6 years can bring up to 73%, but the seed storage overlong time is unfavorable for production application.
In sum, the germinating method of in the past breaking the Leymus chinensis seeds dormancy mainly is simple physics method and chemical method.Promptly 1) physical method mainly comprises simple constant temperature, alternating temperature, and handle in magnetic field, the germinating bed of differing texture (as earth culture, Sha Pei or paper training).2) chemical method has the rare earth seed soaking to handle, exogenous hormone processing etc., and (the just big Chen Yuan of Ma Hongyuan beam improves Leymus chinensis seeds germination rate method progress but action effect is all undesirable.The China meadow, in July, 2005,27 (4): 64-68).
The common drawback of these methods was in the past: 1) lack a unified standard germinating method, comparativity is relatively poor between the data.2) be better than the fact that constant temperature germinates though found the alternating temperature germination, do not find desirable alternating temperature amplitude of a cover and method combination all the time.3) seed that does not have to find to break simply a Leymus chinensis seeds dormancy is arranged (impurity elimination+seed soaking etc.) technology in advance.What 4) adopt mainly is single processing method, arrange the in advance technology and the alternating temperature organic assembling of seed is not got up to create new germinating method and technology.
Summary of the invention
The purpose of this invention is to provide a kind of Leymus chinensis high-temperature seed presoaking and temperature changing combination germinating method.
The present invention is in order to improve the Leymus chinensis seeds germination rate by arranging the control of technology and temperature to combine in advance, create a kind of combination germinating method, its core mainly comprises two parts, promptly 1) seed-soaking method: mainly arrange the quick seed-soaking method of high temperature in the technology partly to remove the Leymus chinensis seeds dormancy in advance with seed.2) germinating method: mainly utilize the alternating temperature technology to improve the Leymus chinensis seeds germination rate fast.Both ingenious combinations have effectively breaks the dormancy of Leymus chinensis seeds severe fast, significantly improves the effect of Leymus chinensis seeds germination rate.
The present invention will arrange technology and temperature control to combine in advance, improve the Leymus chinensis seeds germination rate, specifically realize by following process.
1. seed cleaning impurity elimination
Because the Leymus chinensis seeds grain is little, and ripening rate is lower, the Leymus chinensis seeds after the results generally contains a large amount of impurity, therefore, guarantees that seed purity and cleanliness are to improve the key of Leymus chinensis seeds germination rate.Selected impurity elimination can adopt mechanical or manual method to carry out.Seed purity and cleanliness should reach more than 90%.
2. high-temperature seed presoaking
High-temperature seed presoaking can fast softening kind skin and bran skin, has the water penetration and the air capacity of soils that increase seed, quickens moisture absorption, rapidly the effect of breaking dormancy.
Leymus chinensis seeds after the selected impurity elimination is packed in the permeable gauze bag or nylon wire, added in 33-37 ℃ the water bath with thermostatic control high-temperature seed presoaking 12-48 hour.High-temperature seed presoaking is compared with low-temperature seed presoaking, can significantly shorten seed soaking time, has the effect of rapid raising percentage of seedgermination.Can rationally arrange seed to arrange the time in advance according to the actual requirements on producing.
3. seed disinfection
Take out Leymus chinensis seeds that high-temperature seed presoaking crosses carry out air-dry after, put into the seed disinfectant sterilizing 10-15 minute, thoroughly clean 3-4 time with clean water then and carry out seed sprouting again.Used disinfectant can be 0.1% mercuric chloride solution.
4. alternating temperature germinates
Temperature is the important factor that influences seed sprouting.Generally speaking, suitable temperature environment promotes seed germination, the too high or too low seed germination that all suppresses.For some species, as sheep's hay, even the constant temperature that suits can not be sprouted smoothly, and reasonably the alternating temperature combination can solve this germination difficult problem preferably.
The Leymus chinensis seeds of sterilizing is seeded in the various germinating beds germinating bed such as soil, filter paper, sand germinating bed etc.Be placed on low temperature 5-16 ℃, dark is under the situation of unglazed photograph, 12-16 hour; High temperature 28-35 ℃, under the light conditions, 12-8 hour.The time of low temperature, dark time and high temperature, illumination was to circulate in the cycle with 24 hours, under this alternating temperature condition germination 7-21 days.
The present invention can make the sheep's hay germination rate 69.3% bring up to 84.0% by do not soak seed (soaking seed 0 day).Therefore, the present invention all has important practice significance and value widely to Leymus chinensis seeds cultivation and experimental study.
Embodiment
Embodiment
1. seed cleaning impurity elimination
For guaranteeing to improve the Leymus chinensis seeds germination rate, method is by hand carried out selected impurity elimination, and seed purity and cleanliness are reached more than 90%.
2. high-temperature seed presoaking
Leymus chinensis seeds after the selected impurity elimination is packed in the permeable gauze bag, added in 35 ℃ the water bath with thermostatic control respectively high-temperature seed presoaking 0,3,6,12,24,48,72 hours.
3. seed disinfection
Respectively the Leymus chinensis seeds that high-temperature seed presoaking is crossed air-dry after, put into 0.1% mercuric chloride solution, sterilization 10 minutes is thoroughly cleaned 4 times with clean water then, carries out seed sprouting again.
4. alternating temperature germinates
The Leymus chinensis seeds of above-mentioned sterilization is seeded in respectively on the sand germinating bed.Place 16 ℃ of low temperature, under the situation of dark, 12 hours; 28 ℃ of high temperature, under the light conditions, 12 hours.Under this alternating temperature condition, be to circulate in the cycle with 24 hours, germination 7-21 days.
By the foregoing description, the experimental result that obtains is as shown in table 2.Germinateed the 21st day, 24 hours percentage of seedgermination of 35 ℃ of following high-temperature seed presoakings is the highest, is 84.0%, is significantly higher than contrast promptly 69.3% of seed soaking (soak seed 0 hour), and seed soaking time is above after 24 hours, and germination rate is on a declining curve.But from germination rate, germination vigor, overall target on germination index and the average germinating time is the best with high-temperature seed presoaking germination combination in 12 hours resultant effect.
35 ℃ of high-temperature seed presoaking different times of table 2 are to mainly the germinate influence of index of Leymus chinensis seeds
Annotate: germination vigor calculates with the germination rate that germinateed the 6th day, all the other investigation results for germinateing the 21st day.
Claims (3)
1. Leymus chinensis high-temperature seed presoaking and temperature changing combination germinating method is characterized in that being made of following step:
1) seed cleaning impurity elimination
With machinery or the artificial selected impurity elimination of method, make seed purity and cleanliness should reach more than 90%;
2) high-temperature seed presoaking
Leymus chinensis seeds after the selected impurity elimination is packed in the permeable gauze bag or nylon wire, added in 33-37 ℃ the water bath with thermostatic control high-temperature seed presoaking 12-48 hour;
3) seed disinfection
After the Leymus chinensis seeds that the taking-up high-temperature seed presoaking is crossed is air-dry, put into seed disinfectant, carry out surface disinfection, sterilizing 10-15 minute; Carry out seed sprouting again after thoroughly cleaning 3-4 time with clean water then;
4) alternating temperature germinates
The Leymus chinensis seeds of sterilizing is seeded in the germinating bed, places low temperature 5-16 ℃, under the dark situation, 12-16 hour; Place high temperature 28-35 ℃ again, under the light conditions, 12-8 hour; The time of low temperature, dark time and high temperature, illumination was to circulate in the cycle with 24 hours, under this alternating temperature condition germination 7-21 days.
2. Leymus chinensis high-temperature seed presoaking according to claim 1 and temperature changing combination germinating method is characterized in that the disinfectant that the seed disinfection process is used is 0.1% mercuric chloride solution, and the used germinating bed of alternating temperature germination process is soil, filter paper or sand germinating bed.
3. Leymus chinensis high-temperature seed presoaking according to claim 2 and temperature changing combination germinating method is characterized in that:
1) method by hand is with the seed cleaning impurity elimination;
2) in 35 ℃ water bath with thermostatic control, distinguish high-temperature seed presoaking 12 hours;
3) with seed disinfection 10 minutes in disinfectant, thoroughly clean 4 times with clean water then;
4) Leymus chinensis seeds is seeded on the sand germinating bed, places 16 ℃ of low temperature, 12 hours, 28 ℃ of high temperature 12 hours, germinateed 21 days.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103229650A (en) * | 2013-05-03 | 2013-08-07 | 重庆市药物种植研究所 | Seedling culturing method for limestone plant Cordalis tomentella Franch. |
| CN103477847A (en) * | 2013-09-26 | 2014-01-01 | 镇江万山红遍农业园 | Vegetable plug seedling method |
| CN106613238A (en) * | 2016-12-23 | 2017-05-10 | 庆阳敦博科技发展有限公司 | Dioscorea zingiberensis cultivation method in high attitude area |
| CN111869556A (en) * | 2020-05-22 | 2020-11-03 | 北京林业大学 | Culture method for promoting pigeonpea seeds to rapidly germinate and grow into seedlings |
-
2006
- 2006-12-14 CN CNA2006101632466A patent/CN101199252A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103229650A (en) * | 2013-05-03 | 2013-08-07 | 重庆市药物种植研究所 | Seedling culturing method for limestone plant Cordalis tomentella Franch. |
| CN103477847A (en) * | 2013-09-26 | 2014-01-01 | 镇江万山红遍农业园 | Vegetable plug seedling method |
| CN106613238A (en) * | 2016-12-23 | 2017-05-10 | 庆阳敦博科技发展有限公司 | Dioscorea zingiberensis cultivation method in high attitude area |
| CN106613238B (en) * | 2016-12-23 | 2020-10-02 | 诸暨市物昇农业科技有限公司 | Method for cultivating yellow ginger in high-altitude areas |
| CN111869556A (en) * | 2020-05-22 | 2020-11-03 | 北京林业大学 | Culture method for promoting pigeonpea seeds to rapidly germinate and grow into seedlings |
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Open date: 20080618 |