CN114846946B - Method for improving germination rate of white flower front Hu Chongzi - Google Patents
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- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
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- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
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Abstract
The invention discloses a method for providing germination rate of white flower front Hu Chongzi, which is characterized in that well-developed mature white flower front Hu Guoshi is collected, and seeds are placed indoors for after-ripening and then kneaded for impurity removal for later use; placing the treated seeds in a dark place at the temperature of 4-8 ℃ for preservation at a low temperature; washing seeds with distilled water for 2-3 times before sowing, sterilizing with 3% sodium hypochlorite solution for 15min, and soaking in mixed solution of gibberellin, sodium nitrophenolate and cytokinin for 12h; and (5) storing in sand at a low temperature for 2 weeks, taking out the seeds, cleaning, and sowing the seeds on a germination accelerating matrix. The result shows that the method for improving the germination rate of the white flower front Hu Chongzi provided by the invention ensures that the germination rate of the white flower front Hu Chongzi stored for 8 months after harvesting reaches more than 70% in 28 days of sowing, improves the germination rate, shortens the germination time and is convenient for large-scale artificial cultivation compared with the conventional sowing method.
Description
Technical Field
The invention belongs to the technical field of plant propagation, and particularly relates to a method for improving germination rate of white flower front Hu Chongzi.
Background
Peucedanum praeruptorum (Peucedanum praeruptorum Dunn) is a perennial herb of Peucedanum (Umbelliferae). The Chinese medicinal composition is used as a medicament at the root, has the effects of dispelling wind, dissipating heat, reducing qi, resolving phlegm and the like, and is mainly used for treating cough due to wind-heat, excessive phlegm and asthma, and bronchitis diseases. The peucedanum praeruptorum dunn is a traditional bulk medicinal material in China and is one of the eight new components in Zhejiang province. Since the early peucedanum root is mainly collected by wild, the wild resources are gradually exhausted along with the expansion of market demands. In recent years, the planting area is gradually increased, and the production area is distributed in multiple provinces and cities in China. Seed is the main way of propagation of peucedanum praeruptorum dunn. In the production practice of pharmaceutical farmers, the Hu Chongzi before white flowers are easily influenced by environmental conditions and climatic factors, and the phenomena of low germination rate, irregular germination, prolonged germination period and the like generally exist, so that the bred plants grow differently, the number of plants is small, the production period is long, and the emergence rate is low, thus limiting the mass production of the plants.
Disclosure of Invention
The invention aims to provide a method for improving the germination rate of peucedanum praeruptorum dunn Hu Chongzi aiming at the defects of the existing peucedanum praeruptorum dunn seed propagation technology, which can obviously improve the germination rate of peucedanum praeruptorum dunn Hu Chongzi and is convenient for large-scale artificial planting.
The aim of the invention is realized by the following technical scheme:
a method for increasing germination rate of white flower front Hu Chongzi includes such steps as collecting well-developed mature white flower front Hu Guoshi, indoor laying, kneading to remove impurities, storing at 4-8 deg.C, sterilizing with 3% sodium hypochlorite solution, immersing in mixed solution of gibberellin, sodium nitrophenolate and cytokinin for 12 hr, sand storing at 0-4 deg.C for 2 weeks, and sowing on germination accelerating matrix.
Further, the white flowers Hu Guoshi which are well-developed, mature and full are collected, placed indoors, kneaded to remove impurities after seeds are post-matured, and then stored in a dark place at the temperature of 4-8 ℃, specifically:
in 9-10 months, when the color of the white peucedanum praeruptorum dunn fruit turns to be yellow-white, selecting the Hu Zhu inflorescence of the white peucedanum praeruptorum dunn, cutting off the fruits which have no plant diseases and insect pests and grow into robust plants along with the pedicel, putting the fruits into a breathable woven bag, placing the woven bag in a room, stacking for 3-5 days for after-ripening, wherein the stacking height is not more than 25cm, turning over the fruits at an irregular period during stacking to ensure that the fruits are completely ripe, kneading the fruits after the after-ripening is completely finished, removing impurities, and airing the fruits; and (5) filling the dried seeds into a woven bag and storing the seeds in a dark place at the temperature of 4-8 ℃.
Further, before sowing, the seeds are sterilized by 3% sodium hypochlorite solution and then are soaked in a mixed solution of gibberellin, sodium nitrophenolate and cytokinin for 12 hours, specifically:
washing the selected seeds with distilled water for 2-3 times, soaking in sodium hypochlorite solution with mass concentration of 3% for 15min, taking out, washing with distilled water for 3-5 times, and draining; the drained seeds are soaked in a mixed solution containing 100mg/L gibberellin, 10mg/L compound sodium nitrophenolate and 10mg/L cytokinin for 12 hours, and the seeds are put into a breathable cloth bag after being drained.
Further, the sand storage at 0-4 ℃ for 2 weeks is specifically:
spreading fine sand 5-10cm below the container, spreading the bag with seeds on the fine sand, and covering with fine sand 3-5cm thick, and storing at 0-4deg.C for 2 weeks.
Further, the sowing is carried out on a germination accelerating matrix for germination, specifically: taking out the seeds after sand storage, cleaning the seeds with distilled water, sowing the seeds on a germination accelerating matrix, and covering the germination accelerating matrix with the thickness of 0.3 cm; the germination accelerating matrix is prepared by uniformly mixing flower soil, perlite and vermiculite according to the volume ratio of 3:1:1; the germination condition is 20-25 ℃, the humidity is 70% -80%, and the lighting condition is 12h lighting/12 h darkness.
The beneficial effects of the invention are as follows: the invention adopts the methods of exogenous reagent seed soaking and low-temperature sand storage treatment to improve the germination rate of the peucedanum praeruptorum dunn, improve the uniformity of emergence, ensure uniform emergence size, facilitate manual large-scale planting, and have simple method and easy popularization.
Detailed Description
The invention provides a method for improving germination rate of white flower front Hu Chongzi, which improves the germination rate of white flower front Hu Chongzi by sterilizing, soaking seeds with exogenous reagent and low-temperature sand storage treatment for one time, and specifically comprises the following steps:
1) Seed collection: the fruits of the peucedanum praeruptorum dunn are generally ripe for 9-10 months, when the color of the peucedanum praeruptorum dunn is changed into yellow and white, the inflorescence of the peucedanum praeruptorum dunn Hu Zhu is selected, the fruits which have no plant diseases and insect pests and grow into strong plants are cut off together with the pedicel, the fruits are put into a breathable woven bag, the woven bag is placed in a room, the woven bag is piled for 3-5 days for after-ripening, the piling height is not more than 25cm, the fruits are turned over at an irregular period during piling, the fruits are ensured to be completely ripe, and after the after-ripening is complete (full seeds and brown), the fruits are kneaded, impurities are removed and the woven bag is dried for standby.
2) Preserving at low temperature: and (5) filling the treated seeds into a woven bag, and preserving the seeds in a dark place at the temperature of 4-8 ℃.
3) Sterilizing and soaking seeds: washing the selected seeds with distilled water for 2-3 times, soaking in sodium hypochlorite solution with mass concentration of 3% for 15min, taking out, washing with distilled water for 3-5 times, and draining; soaking the drained seeds in Gibberellin (GA) 3 100 mg/L), sodium nitrophenolate (10 mg/L) and cytokinin (6-BA 10 mg/L) for 12h, draining, and packaging into a breathable cloth bag.
4) Low Wen Shacang: spreading fine sand with thickness of 5-10cm at the bottom of the container, spreading a cloth bag containing seeds on the fine sand, covering with fine sand with thickness of 3-5cm, and storing at low temperature for 2 weeks at 0-4deg.C.
5) And (3) germination treatment: taking out the seeds, cleaning with distilled water, sowing the seeds on the germination accelerating matrix, and covering the germination accelerating matrix with the thickness of 0.3 cm. The germination accelerating matrix is prepared by uniformly mixing flower soil, perlite and vermiculite according to the volume ratio of 3:1:1; the germination condition is 20-25 ℃, the humidity is 70% -80%, and the lighting condition is 12h lighting/12 h darkness.
The objects and effects of the present invention will become more apparent from the following detailed description of specific embodiments thereof. The reagents used in the examples described below, unless otherwise specified, were purchased from conventional biochemical reagent stores. Quantitative data in the following examples were set up in triplicate and the results averaged.
Example 1:
a method for increasing germination rate of white flower front Hu Chongzi, the method comprising the steps of:
1) Seed collection: the fruits of the peucedanum praeruptorum dunn are generally ripe for 9-10 months, when the color of the peucedanum praeruptorum dunn is changed into yellow and white, the inflorescence of the peucedanum praeruptorum dunn Hu Zhu is selected, the fruits which have no plant diseases and insect pests and grow into strong plants are cut off together with the pedicel, the fruits are put into a breathable woven bag, the woven bag is placed in a room, the woven bag is piled for 3-5 days for after-ripening, the piling height is not more than 25cm, the fruits are turned over at an irregular period during piling, the fruits are ensured to be completely ripe, and after the after-ripening is complete (full seeds and brown), the fruits are kneaded, impurities are removed and the woven bag is dried for standby.
2) Preserving at low temperature: and (5) placing the treated seeds into a woven bag, and storing the seeds for 8 months at the temperature of 4-8 ℃ in a dark place.
3) And (3) disinfection: soaking the selected seeds in 3% sodium hypochlorite solution for 10min, taking out, washing with distilled water for 3 times, and draining.
4) Low Wen Shacang: spreading fine sand with thickness of 5-10cm at the bottom of the container, spreading a cloth bag containing seeds on the fine sand, covering with fine sand with thickness of 3-5cm, and storing at low temperature for 2 weeks at 0-4deg.C.
5) And (3) germination treatment: taking out the seeds, cleaning with distilled water, sowing the seeds on the germination accelerating matrix, and covering the germination accelerating matrix with the thickness of 0.3 cm. The germination accelerating matrix is prepared by uniformly mixing flower soil, perlite and vermiculite according to the volume ratio of 3:1:1; the germination condition is 20-25 ℃, the humidity is 70% -80%, and the lighting condition is 12h lighting/12 h darkness.
Comparative example 1:
the comparative example of the invention provides a germination method of peucedanum praeruptorum dunn seeds, the specific process of which is similar to that of example 1, and the difference is that the seeds are not stored at the temperature of 4-8 ℃ for Wen Biguang after being collected, but are stored at the shade place at the normal temperature for 8 months. The temperature, humidity and illumination control in the subsequent treatment and seedling germination process are the same as in example 1.
Example 2:
a method for increasing germination rate of white flower front Hu Chongzi, the method comprising the steps of:
1) Seed collection: the fruits of the peucedanum praeruptorum dunn are generally ripe for 9-10 months, when the color of the peucedanum praeruptorum dunn is changed into yellow and white, the inflorescence of the peucedanum praeruptorum dunn Hu Zhu is selected, the fruits which have no plant diseases and insect pests and grow into strong plants are cut off together with the pedicel, the fruits are put into a breathable woven bag, the woven bag is placed in a room, the woven bag is piled for 3-5 days for after-ripening, the piling height is not more than 25cm, the fruits are turned over at an irregular period during piling, the fruits are ensured to be completely ripe, and after the after-ripening is complete (full seeds and brown), the fruits are kneaded, impurities are removed and the woven bag is dried for standby.
2) Preserving at low temperature: and (5) placing the treated seeds into a woven bag, and storing the seeds for 8 months at the temperature of 4-8 ℃ in a dark place.
3) Sterilizing and soaking seeds: washing the selected seeds with distilled water for 2-3 times, soaking in sodium hypochlorite solution with mass concentration of 3% for 15min, taking out, washing with distilled water for 3-5 times, and draining; immersing the drained seeds in sterile water for 12, draining, and filling into a breathable cloth bag.
4) Low Wen Shacang: spreading fine sand with thickness of 5-10cm at the bottom of the container, spreading a cloth bag containing seeds on the fine sand, covering with fine sand with thickness of 3-5cm, and storing at low temperature for 2 weeks at 0-4deg.C.
5) And (3) germination treatment: taking out the seeds, cleaning with distilled water, sowing the seeds on the germination accelerating matrix, and covering the germination accelerating matrix with the thickness of 0.3 cm. The germination accelerating matrix is prepared by uniformly mixing flower soil, perlite and vermiculite according to the volume ratio of 3:1:1; the germination condition is 20-25 ℃, the humidity is 70% -80%, and the lighting condition is 12h lighting/12 h darkness.
Comparative example 2:
the comparative example of the invention provides a germination method for peucedanum praeruptorum dunn seeds, and the specific process is similar to that of example 2, and the difference is only that the seeds are directly sown in a germination accelerating matrix for germination treatment after disinfection and seed soaking. The temperature, humidity and illumination control in the seedling germination process are the same as in example 2.
Example 3:
a method for increasing germination rate of white flower front Hu Chongzi, the method comprising the steps of:
1) Seed collection: the fruits of the peucedanum praeruptorum dunn are generally ripe for 9-10 months, when the color of the peucedanum praeruptorum dunn is changed into yellow and white, the inflorescence of the peucedanum praeruptorum dunn Hu Zhu is selected, the fruits which have no plant diseases and insect pests and grow into strong plants are cut off together with the pedicel, the fruits are put into a breathable woven bag, the woven bag is placed in a room, the woven bag is piled for 3-5 days for after-ripening, the piling height is not more than 25cm, the fruits are turned over at an irregular period during piling, the fruits are ensured to be completely ripe, and after the after-ripening is complete (full seeds and brown), the fruits are kneaded, impurities are removed and the woven bag is dried for standby.
2) Preserving at low temperature: and (5) placing the treated seeds into a woven bag, and storing the seeds for 8 months at the temperature of 4-8 ℃ in a dark place.
3) Sterilizing and soaking seeds: washing the selected seeds with distilled water for 2-3 times, soaking in sodium hypochlorite solution with mass concentration of 3% for 15min, taking out, washing with distilled water for 3-5 times, and draining; the drained seeds are soaked in a mixed solution of gibberellin (GA 3100 mg/L), compound sodium nitrophenolate (10 mg/L) and cytokinin (6-BA 10 mg/L) for 12 hours, and the seeds are put into a breathable cloth bag after being drained.
4) Low Wen Shacang: spreading fine sand with thickness of 5-10cm at the bottom of the container, spreading a cloth bag containing seeds on the fine sand, covering with fine sand with thickness of 3-5cm, and storing at low temperature for 2 weeks at 0-4deg.C.
5) And (3) germination treatment: taking out the seeds, cleaning with distilled water, sowing the seeds on the germination accelerating matrix, and covering the germination accelerating matrix with the thickness of 0.3 cm. The germination accelerating matrix is prepared by uniformly mixing flower soil, perlite and vermiculite according to the volume ratio of 3:1:1; the germination condition is 20-25 ℃, the humidity is 70% -80%, and the lighting condition is 12h lighting/12 h darkness.
Comparative example 3:
the comparative example provided a method for germination of Peucedanum praeruptorum dunn seeds, which is similar to example 3 in specific process, except that seeds are sterilized and drained and then soaked in gibberellin (GA 3100 mg/L) solution for 12 hours, and then put into a breathable cloth bag. The temperature, humidity and illumination control in the seedling germination process are the same as in example 3.
Comparative example 4:
the comparative example of the invention provides a germination method of peucedanum praeruptorum dunn seeds, the specific process of which is similar to that of example 3, except that the seeds are soaked in a compound sodium nitrophenolate (10 mg/L) solution for 12 hours after disinfection and draining, and then are put into a breathable cloth bag after draining. The temperature, humidity and illumination control in the seedling germination process are the same as in example 3.
Comparative example 5:
the comparative example of the invention provides a germination method of peucedanum praeruptorum dunn seeds, which has the specific process similar to that of example 3, except that the seeds are soaked in cytokinin (6-BA 10 mg/L) solution for 12 hours after disinfection and draining, and then are put into a breathable cloth bag after draining. The temperature, humidity and illumination control in the seedling germination process are the same as in example 3.
The germination of the seeds was recorded every 48h after sowing until 28d was observed, the results of which are shown in table 1. Seed germination is marked by radicle extending out of the seed coat (white). The germination rate calculation formula is as follows:
germination percentage (%) = (total number of final germinated seeds/total number of test seeds) ×100%
TABLE 1
As can be seen from the results of table 1, in example 1, compared with comparative example 1, the temperature condition of storage has a significant effect on germination of peucedanum praeruptorum dunn seeds, if the storage time is longer, the seeds need to be stored in a dark place at 4-8 ℃, otherwise, the vigor of the seeds is lost, so that the seeds cannot germinate; example 2 compared with comparative example 2, the germination rate of the peucedanum praeruptorum dunn seeds can be improved to a certain extent through low-temperature sand storage; example 3 has an effect of promoting germination by using gibberellin, sodium nitrite and cytokinin solutions alone, which are used to soak the white flowers before Hu Chongzi, compared with comparative example 3, comparative example 4 and comparative example 5, wherein the germination rate of the gibberellin treated seeds is 65% higher than that of the sodium nitrite and cytokinin treated individually, and the mixed solution of gibberellin (GA 3100 mg/L), sodium nitrophenolate (10 mg/L) and cytokinin (6-BA 10 mg/L) can more remarkably improve the germination rate of seeds to 73% and the germination time is advanced to day 5, which is superior to the other three control groups.
The foregoing is a further detailed description of the invention in connection with the preferred embodiments, and it is not intended that the invention be limited to the specific embodiments described. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (4)
1. A method for improving germination rate of white flower front Hu Chongzi is characterized in that white flower front Hu Guoshi which is well developed and full is collected, seeds are placed indoors for after ripening, kneaded for impurity removal and then stored in a dark place at 4-8 ℃, the seeds are sterilized by 3% sodium hypochlorite solution before sowing, then are soaked in a mixed solution of gibberellin, sodium nitrophenolate and cytokinin for 12 hours, and are sowed on a germination accelerating matrix for germination after sand storage for 2 weeks at 0-4 ℃; before sowing, the seeds are sterilized by 3% sodium hypochlorite solution and then soaked in a mixed solution of gibberellin, sodium nitrophenolate and cytokinin for 12 hours, specifically:
washing the selected seeds with distilled water for 2-3 times, soaking in sodium hypochlorite solution with mass concentration of 3% for 15min, taking out, washing with distilled water for 3-5 times, and draining; the drained seeds were soaked in a mixed solution containing 100mg/L gibberellin, 10mg/L sodium nitrophenolate, and 10mg/L cytokinin for 12 hours.
2. The method for improving germination rate of white flower front Hu Chongzi according to claim 1, wherein the collecting well-developed mature white flower front Hu Guoshi is stored in a dark place at 4-8deg.C after seed is left in the room for after-ripening and then kneaded to remove impurities, specifically:
in 9-10 months, when the color of the white peucedanum praeruptorum dunn fruit turns to be yellow-white, selecting the Hu Zhu inflorescence of the white peucedanum praeruptorum dunn, cutting off the fruits which have no plant diseases and insect pests and grow into robust plants along with the pedicel, putting the fruits into a breathable woven bag, placing the woven bag in a room, stacking for 3-5 days for after-ripening, wherein the stacking height is not more than 25cm, turning over the fruits at an irregular period during stacking to ensure that the fruits are completely ripe, kneading the fruits after the after-ripening is completely finished, removing impurities, and airing the fruits; and (5) filling the dried seeds into a woven bag and storing the seeds in a dark place at the temperature of 4-8 ℃.
3. The method for improving germination rate of pre-white flowers Hu Chongzi according to claim 1, wherein the sand storage at 0-4 ℃ for 2 weeks is specifically:
spreading fine sand 5-10cm below the container, spreading the bag with seeds on the fine sand, and covering with fine sand 3-5cm thick, and storing at 0-4deg.C for 2 weeks.
4. The method for improving germination rate of pre-white flowers Hu Chongzi according to claim 1, wherein the sowing onto the germination accelerating substrate is used for germination, specifically: taking out the seeds after sand storage, cleaning the seeds with distilled water, sowing the seeds on a germination accelerating matrix, and covering the germination accelerating matrix with the thickness of 0.3 cm; the germination accelerating matrix is prepared by uniformly mixing flower soil, perlite and vermiculite according to the volume ratio of 3:1:1; the germination condition is 20-25 ℃, the humidity is 70% -80%, and the lighting condition is 12h lighting/12 h darkness.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210662210.1A CN114846946B (en) | 2022-06-13 | 2022-06-13 | Method for improving germination rate of white flower front Hu Chongzi |
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| CN202210662210.1A CN114846946B (en) | 2022-06-13 | 2022-06-13 | Method for improving germination rate of white flower front Hu Chongzi |
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