CN107360756A - It is a kind of to promote garcinia paucinervis seed quick and the method for multiple Germination And Seedling - Google Patents
It is a kind of to promote garcinia paucinervis seed quick and the method for multiple Germination And Seedling Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0299—Handling or transporting of soil blocks or seedlings
Abstract
The invention discloses a kind of method for promoting garcinia paucinervis seed to sprout and repeatedly breed, pass through each conditions for synthetically controlling garcinia paucinervis seed to sprout, break seed dormancy, improve speed of germinating, regularity and the germination rate of seed, significantly seed is promoted to sprout, while the characteristic that can be repeatedly sprouted using its seed carries out multiple batches of nursery, growing-seedling period can be greatly shortened in this method, the production seedling rate of seed is improved, reduces seedling cost.Originally 85.0% garcinia paucinervis seed is can be only achieved in 350 days germination rates, after this method is handled, the germination rate first of 30 days can reach 95.0%, and the seed of recovery is sprouted fast and neat by vernalization in 60 days, germination percentage up to more than 83.3%.This method has established good basis for the efficient breeding of garcinia paucinervis nursery stock, has very active influence to rare or endangered species resource regeneration and sustainable utilization.
Description
Technical field
It is specifically a kind of to promote garcinia paucinervis seed quick and the side of multiple Germination And Seedling the invention belongs to biological technical field
Method.
Background technology
Garcinia paucinervis（Garcinia paucinervis）For Guttiferae（Guttiferae）Garcinia（Garcinia）Arbor,
Gained the name because radial spun gold sample ray from inside to outside can be seen for its trunk cross section.It is distributed mainly on West Guangxi,
It is the peculiar rare tree of tor, its is tree-like on south and 194 ~ 830m of southeastern Yunnan height above sea level limestone karst features mountain region
Tall and straight grace, branches and leaves gloss is evergreen, tender leaf is scarlet, is excellent color leaf ornamental tree species.《Chinese draft》Record, the branch of garcinia paucinervis
Leaf, bark can make medicinal material, have the effect of clearing heat and detoxicating, detumescence, cure mainly carbuncle sore tumefacting virus, burn, scald etc., for the strong medicine of tradition.It
Still " one of five big hardwood of Guangxi ", its wooden hard, compact structure, texture straight, corrosion resistant is water-fast and anti-insect, is that Guangxi writes
The hophornbeam of name is high for machinery, military affairs, shipbuilding, building and noble furniture, economic value.Because garcinia paucinervis habitat is fragile, point
Layout limited and scattered, natural regeneration is difficult, and indiscriminate felling of trees phenomenon is serious etc., IUCN is classified as VU level species, is《It is Chinese precious
Dilute imminent extinction protected plants register》In two level national key protected plant and《Chinese Plants Red Data Book》In susceptible species, be also logged
《Zhuang nationality in Guangxi autonomy Top-rated protected wild plants register》.
The recovery of karst vegetation needs the reserve of a large amount of seedling.Garcinia paucinervis is north tropic and south subtropicses tor
The indigenous tree in area, the ecological environment of locality is adapted to, revegetation and urban greening are carried out to this area using the seeds, but
The biggest problem faced is that resource regeneration is slow, and wild resource can not be excavated, as a kind of excellent native country Precious, Rare, Endangered
Ornamental plant and medicinal material, resource seedling are far from enough.Garcinia paucinervis cutting propagation is extremely difficult, and its seed has dormancy, sprouts
Very slowly, conventional sowing method can not change the very long situation of garcinia paucinervis Seed Germination, and these, which turn into, restricts spun gold
The bottleneck of Lee's artificial growth.And learn the big tree of garcinia paucinervis and there is that solid plant is few in field investigation, seed resource is valuable,
Therefore it is to introduce a fine variety the key for promoting garcinia paucinervis to carry out nursery using limited seed.Existing research focuses mostly in garcinia paucinervis at present
Chemical composition, pharmacological effect etc., have no the report on garcinia paucinervis proliferation.And have in garcinia mangostana, spend more mountain
Bamboo etc. belongs to the research that other plant carries out seed propagation together, but studies or require high tissue cultures to Reproduction Conditions, or
It is to promote to sprout using only growth regulator seed soaking.We are had found by studying, and are grown and are adjusted using only various concentrations
Saving agent is promoting garcinia paucinervis seed to sprout DeGrain, and it is very long to sprout the cycle.And seed only utilizes in conventional seedbed system method
Once, i.e., a seed can only cultivate one plant of seedling, it is impossible to fully utilize limited seed resource.
Seed is sprouted can a variety of outsides such as light, temperature, moisture, culture matrix, depth of planting and exogenous hormone and its concentration
The influence of condition element, single_factor method can be used to find out each condition that suitable garcinia paucinervis seed is sprouted, then these conditions are entered
Row combination, seed dormancy can be broken by seeking one, seed is sprouted the combination condition for reaching optimum state, in addition also using kind
The power of regeneration of son carries out repeatedly sprouting nursery.
The content of the invention
Quick it is an object of the invention to provide promotion garcinia paucinervis seed and multiple Germination And Seedling method, by being sprouted to seed
The many factors of clockwork spring part are controlled to promote seed to sprout, and are improved the speed of germinating, regularity and germination rate of seed, are utilized seed
Power of regeneration and storage nutrient, it is reused and repeatedly bred, for rare or endangered species garcinia paucinervis nursery stock it is numerous
Grow and lay the foundation.
The present invention be it is a kind of promote garcinia paucinervis seed quick and the method for multiple Germination And Seedling, including following condition and step
Suddenly：
（1）The preparation of culture matrix：
Culture matrix is river sand, one week before the use, soaks culture matrix with 0.5% carbendazim solution sprinkling, carries out disinfection
Handle, dried under natural conditions with standby；
（2）The preparation of seed：
The spun gold plum fruit adopted back is macerated into a couple of days in outdoor leaching, fruit pulp is washed by rubbing with the hands, the net seed of clear water rinsing, rejects obvious
After pest and disease damage seed, 0.1%KMnO is used4Soaking disinfection 30min, clear water are rinsed well, in case germination is used；
（3）Sprout culture：
The river sand that the thick water content of 2 ~ 2.5cm is 15.0% is spread in culture vessel, seed is equably placed on to river sand surface
On, light pressure makes it be contacted with wet sand, covered container lid, and diameter 5mm air-vent is set in container cover；Again by culture vessel
It is placed in artificial climate incubator and cultivates, is suitably kept the skin wet according to culture medium dehydration situation afterwards, be maintained at its water content
15% or so；
The condition of culture of incubator is：32 DEG C of constant temperature, intensity of illumination 3000lx, light application time 12 h days- 1；
（4）First seedling replanting：
Sowing 60 days or so, nursery stock sprouts substantially and length is to certain altitude, now opens the container cover of constraint growth, uses preservative film
It is overlying on container, to reduce moisture evaporation；
Treat sowing 90 days or so, seedling tender stem by red turn it is green when, the seed being deposited on seedling is cut with the scissors sterilized, carry out
Recovery；Fresh plant ash is smeared in the wound for having wiped out the seedling of seed, and is transplanted in time in nutritive cube；
（5）Second of sowing：
The seed of recovery is sowed, by step（3）Carry out；
（6）Second batch seedling replanting：
Seed to be reclaimed to sow 60 days or so, nursery stock sprouts and long to certain altitude substantially, now opens the container cover of constraint growth,
It is overlying on preservative film on container, to reduce moisture evaporation；And optionally suitably keep the skin wet, river sand water content is maintained at 15%
Left and right；
Continue culture 120 days or so, seedling tender stem by red turn it is green when, the seed being deposited on seedling is cut with the scissors sterilized,
Reclaimed again；Fresh plant ash is smeared in the wound for having wiped out the seedling of seed, and is transplanted to nutritive cube culture in time；
（7）Seed soaking：
The seed of secondary recovery is placed in clean basin, adds 500mg/L gibberellin（GA3）Treatment fluid, do not had seed, often
The lower seed soaking 24h of temperature, clear water are cleaned repeatedly；
（8）Third time is sowed：
Secondary recovery and the seed of seed soaking are sowed, by step（3）Carry out；
（9）3rd batch of seedling replanting：
After planting 90 days or so, the seed of secondary recovery was sprouted and grows to certain altitude, can be by seedling and the kind being deposited on seedling
One piece of son is transplanted into nutritive cube culture.
Garcinia paucinervis is sprouted and higher temperatures are liked in growth, and through experiment, 32 DEG C are optimum temperature.It is aqueous when with river sand being matrix
Amount holding about 15% is most fast through speed of germinating under the conditions of testing this.If seed quality is good, step is repeated（5）With（6）, increase back
Number is received, but the growing way of the more seedling of vernalization number is weaker.
Using the inventive method：After seed implantation carries out sprouting culture, observe daily and count germination seed number, with meat
Red young shoot is arrived soon judges whether seed is sprouted for standard.Sprouted if being sprouted without seed within continuous 30 days and being considered as this batch of seed
Terminate.
Germination percentage (%)=normal chitting piece grain number/total grain number × 100% of seed of participating in the experiment；
Germinating energy (%)=normal chitting piece grain number during normal arrival peak/total grain number × 100% of seed of participating in the experiment；
Averagely sprout duration（My god）=∑(t×n)/∑n, number of days when t is since the sprouting test in formula, n is to be sprouted in t days
Seed number.
The processing that first carried out disinfection to seed and culture matrix is needed using this method, avoids seed mouldy in culture is sprouted.
Seed need to be sowed as early as possible, and storage can reduce germinating capacity under normal temperature.
In addition, germination process is kept, seed is ventilated, and container cover need to box out ventilated.Sowing only need to be seed every time
It is placed on river sand surface to press lightly on, not seed covering, seed covering can increase sprout time.
It is using the effect of gibberellin seed soaking for the last time：Seed speed of germinating is speeded, but uses plant growth regulator
Seedling and young root branch is set to increase and make plant thin and weak while accelerating and sprouting, so suggesting only reclaiming seed in last time
Soaked seed during vernalization using plant growth regulator, one piece of transplanting of the seed that will be deposited in after seedling on seedling, the interior residual of seed
Nutrient can be supplied to growth of seedling.
The inventive method：For garcinia paucinervis seed, germination process is long under field conditions (factors), irregular situation, using Dan Yin
Plain method finds out each condition for being best suitable for sprouting, then these conditions is merged, comprehensive discussion temperature, illumination, culture matrix, matrix
The effect that water content, depth of planting and exogenous hormone and its concentration are sprouted to garcinia paucinervis seed, it have found a garcinia paucinervis seed
The optimal factor combination sprouted, and reuse seed and repeatedly sprouted.Sprout time can be greatly shortened in the method, a collection of
Seed can breed 3 ~ 5 batches of seedling, and for every batch of emergence rate up to more than 83.3%, this method can significantly reduce seedling cost, and have
The advantages of convenience, of low pollution, low cost.
Originally sprout under field conditions (factors) extremely slow garcinia paucinervis seed it is treated by the present method after, open within after planting about 6 days
Begin to sprout, reach the peak sprouted within 14 days or so, at 30 days, germination rate can reach more than 90%, and average sprout time is 12.6
My god, germination is fast and neat.After planting 90 days or so first, average plant height was up to 8.7cm, root long 12.5cm, base footpath 2.0mm, master
The thick 2.1mm of root, the number of blade 3.6；After seed recovery, the seed germination rate of second and third sowing is average up to more than 83.3%
Sprout time is respectively 35.2 days, 31.3 days, is sprouted fast and neat.After planting 90 days, the average plant height of seedling of second of sowing
7.4cm, root long 8.8cm, the average plant height 7.2cm, root long 4.8cm of seedling of third time sowing.It is 90 days to receive the seedling time for the first time,
Second is 120 days, and third time can be completed to breed three batches of seedling in 90 days, altogether 300 days, 1 year.
The inventive method has established good basis for the efficient breeding of garcinia paucinervis nursery stock, to rare or endangered species resource regeneration
There is very active influence with sustainable utilization.
Brief description of the drawings
Fig. 1 is the step of embodiment 1（3）Seed sprouts culture schematic diagram；
Fig. 2 is the step of embodiment 1（4）First growth of seedling schematic diagram；
Fig. 3 is the step of embodiment 1（4）The growing way schematic diagram of first seedling；
Fig. 4 is the step of embodiment 1（6）Second batch growth of seedling schematic diagram；
Fig. 5 is the step of embodiment 1（6）The growing way schematic diagram of second batch seedling；
Fig. 6 is the step of embodiment 1（9）3rd batch of growth of seedling schematic diagram；
Fig. 7 is the step of embodiment 1（9）The growing way schematic diagram of 3rd batch of seedling；
Fig. 8 is the step of embodiment 4（5）Seed growing way schematic diagram after seed soaking.
Embodiment
With reference to embodiment, the present invention is further illustrated, but is not limitation of the invention.
Embodiment 1
（1）The preparation of culture matrix：
Culture matrix is river sand, one week before the use, soaks culture matrix with 0.5% carbendazim solution sprinkling, carries out disinfection
Handle, dried under natural conditions with standby；
（2）The preparation of seed：
The spun gold plum fruit adopted back is macerated into a couple of days in outdoor leaching, fruit pulp is washed by rubbing with the hands, the net seed of clear water rinsing, rejects obvious
After pest and disease damage seed, 0.1%KMnO is used4Soaking disinfection 30min, clear water are rinsed well, in case germination is used；
（3）Sprout culture：
As shown in figure 1, culture vessel is length（17.2cm）× wide（11.7cm）× high（7cm）, capacity 1000ml disposable meal
Box, the river sand that the thick water content of 2 ~ 2.5cm is about 15.0% is spread in cutlery box, seed is equably placed on river sand surface, gently
Pressure makes it be contacted with wet sand, covers lid（12 diameter 5mm air-vent is set on lid）, it is placed in artificial climate incubator
Culture, condition of culture are：32 DEG C of constant temperature, periodicity illumination（Intensity of illumination 3000lx, light application time 12 h days- 1）, it
Suitably kept the skin wet according to culture medium dehydration situation afterwards, its water content is maintained at 15% or so；
（4）First seedling replanting：
As Figure 2-3, after planting 60 days, nursery stock sprouted and grown the lid for certain altitude, now opening constraint growth substantially,
It is overlying on preservative film on cutlery box, to reduce moisture evaporation；
Treat sowing 90 days or so, seedling tender stem by red turn it is green when, the seed being deposited on seedling is cut with the scissors sterilized, carry out
Recovery；Fresh plant ash is smeared in the wound for having wiped out the seedling of seed, and is transplanted in time in nutritive cube；
（5）Second of sowing：
The seed of recovery is sowed, by step（3）Carry out；
（6）Second batch seedling replanting：
As illustrated in figures 4-5, after seed recovery is sowed about 60 days, nursery stock sprouts and grown to certain altitude substantially, now opens lid,
It is overlying on cutlery box with preservative film, is optionally suitably kept the skin wet, river sand water content is maintained at 15% or so.Continue culture to 120
It or so, tender stem by red turn it is green when, the seed being deposited on seedling is cut with the scissors sterilized, is reclaimed again；Wiping out
Fresh plant ash is smeared in the wound of the seedling of seed, is transplanted in time in nutritive cube；
（7）Seed soaking：
The seed of secondary recovery is placed in clean basin, adds 500mg/L gibberellin（GA3）Treatment fluid, do not had seed, often
The lower seed soaking 24h of temperature, clear water are cleaned repeatedly；
（8）Third time is sowed：
Secondary recovery and the seed of seed soaking are sowed, by step（3）Carry out；
（9）3rd batch of seedling replanting：
As shown in fig. 6-7, after planting 90 days, the seed of secondary recovery was sprouted and grows to certain altitude, by seedling and can be deposited in
One piece of seed on seedling is transplanted into nutritive cube culture.
As a result：Sow first, after planting 6 days start to sprout, and the peak sprouted is reached at 14 days, and it is 60.0% that germinating energy, which reaches,
At 30 days, germination rate can reach 95.0%, and a length of 12.6 days when averagely sprouting, germination is neat and rapid, and seedling and young root are sturdy；
The seed of germination by repetition starts to sprout for 20 days after broadcasting, and reaches within 30 days and sprouts peak, germinating energy 51.7%, germination percentage reaches after 60 days
To 90.0%, a length of 35.2 days when averagely sprouting, seedling and young root are more sturdy；The seed sprouted three times starts to sprout after broadcasting after 14 days
Hair, the peak sprouted is reached within 21 days or so, germinating energy 48.3%, germination percentage is a length of when averagely sprouting up to 83.3% after 60 days
31.3 days, for part seedling in state of growing thickly, seedling and root system were thinner and more delicate.
Embodiment 2
（1）The preparation of culture matrix：It is same as Example 1；
（2）The preparation of seed：It is same as Example 1；
（3）Sprout culture：
The river sand that the thick water content of 4cm is about 15.0% is spread in cutlery box, 1cm during the seed embedment that seed soaking is crossed is husky, stand
It is flat, it is placed in artificial climate incubator and cultivates, condition of culture is：25 DEG C of constant temperature, periodicity illumination（Intensity of illumination 3000lx,
Light application time 12 h days- 1）, suitably kept the skin wet according to culture medium dehydration situation, water content is maintained at 15.0% or so.
As a result：Start to sprout within after planting 38 days, reach the peak sprouted within 280 days, sprouting in 350 days terminates, germination percentage
For 85.0%, a length of 193.7 days when averagely sprouting, irregular and process of germinateing is very long.
Embodiment 3
（1）The preparation of culture matrix：It is same as Example 1；
（2）The preparation of seed：It is same as Example 1；
（3）Seed soaking：
Ready seed is placed in clean water basin, adds 100mg/L heteroauxins（IAA）Treatment fluid, do not had seed, often
Cleaned up after 24 h of the lower seed soaking of temperature；
（4）Sprout culture：
Except river sand water content is maintained at 20.0% or so, remaining cultural method is the same as the step of embodiment 2（3）.
As a result：Start to sprout within after planting 60 days, reach within 280 days and sprout peak, sprouting in 350 days terminates, and germination percentage is
56.7%, a length of 232.7 days when averagely sprouting, germination rate is low, and germination is irregular and extremely slow.
Embodiment 4
（1）The preparation of culture matrix：Culture matrix is peat soil, is disinfected same as Example 1；
（2）The preparation of seed：It is same as Example 1；
（3）Sprout culture：
Except culture matrix is peat soil, its moistening need to be often kept, remaining and the step of embodiment 2（3）It is identical；
（4）First sprouts seedling replanting：
Change two time points：Take off the container cover time about 300 days, cut the seed being deposited on seedling when about 350 days；It is remaining
With the step of embodiment 1（4）It is identical；
（5）Seed soaking：
The seed for cutting recovery is placed in clean basin, adds 80mg/L 6-benzyl aminopurine（6-BA）Treatment fluid, do not had
Seed, soaked seed under normal temperature 24h, and clear water is cleaned repeatedly, and seed growing way is as shown in Figure 8；
（6）Reclaim seed sowing：
To the seed sowing reclaimed and soaked seed and the step of embodiment 1（3）It is identical；
（7）Second batch seedling replanting：
The step of with embodiment 1（9）It is identical.
As a result：Start to sprout within after planting 39 days first, reach the peak sprouted within 294 days, sprouting in 350 days terminates, and sends out
Bud rate reaches 85.0%, and a length of 221.7 days when averagely sprouting, germination is irregular and slow；Seed after recovery is broadcast latter 16 days
Start to sprout, reach within 21 days and sprout peak, germinating energy 51.7%, the germination percentage of 70 days reaches 95.0%, average sprout time
For 30.0 days, the germination of recovery was fast and neat, but seedling, in state of growing thickly, seedling and root system are thin and delicate, or even only long seedling
Not root of hair.
Table 1 below shows influence of the different disposal to garcinia paucinervis seed sprouting state in embodiment 1 to 4.
Influence of the different disposal of table 1 to garcinia paucinervis seed sprouting state
Consider initial sprout time, reach the time for sprouting peak, germinating energy, sprout end time, germination percentage, averagely sprout
Duration, germination regularity, seedling and young root branch amount and 9 indexs of healthy and strong degree are sent out, garcinia paucinervis, which sprouts the best processing of effect, is：
Periodicity illumination（Intensity of illumination 3000lx, light application time 12 h days- 1）, 32 DEG C, culture matrix is river sand, covers punching
Container cover, after 90 days or so a collection of seedling of harvest, recovery seed continues to sow, and can harvest second batch seedling within 120 days or so；The
Concentration 500mg/L gibberellin seed soaking, can receive batch seedling of winning the third place for 90 days or so before sowing three times.The method is not only young three times
The germination percentage of seedling is high, and up to 83.3% ~ 95%, other each indexs are also preferable.Reason is clicked here, can obtain within 300 days three batches of seedling, and
Conventional seed planting only obtains a collection of seedling for 350 days, and breeding efficiency is more than 3 times of conventional seedbed system.
Claims (1)
- Promote garcinia paucinervis seed quick and the method for multiple Germination And Seedling 1. a kind of, including following condition and step：（1）The preparation of culture matrix：Culture matrix is river sand, one week before the use, soaks culture matrix with 0.5% carbendazim solution sprinkling, carries out disinfection Handle, dried under natural conditions with standby；（2）The preparation of seed：The spun gold plum fruit adopted back is macerated into a couple of days in outdoor leaching, fruit pulp is washed by rubbing with the hands, the net seed of clear water rinsing, rejects obvious After pest and disease damage seed, 0.1%KMnO is used4Soaking disinfection 30min, clear water are rinsed well, in case germination is used；（3）Sprout culture：The river sand that the thick water content of 2 ~ 2.5cm is 15.0% is spread in culture vessel, seed is equably placed on to river sand surface On, light pressure makes it be contacted with wet sand, covered container lid, and diameter 5mm air-vent is set in container cover；Again by culture vessel It is placed in artificial climate incubator and cultivates, is suitably kept the skin wet according to culture medium dehydration situation afterwards, be maintained at its water content 15% or so；The condition of culture of incubator is：32 DEG C of constant temperature, intensity of illumination 3000lx, light application time 12 h days- 1；（4）First seedling replanting：Sowing 60 days or so, nursery stock sprouts substantially and length is to certain altitude, now opens the container cover of constraint growth, uses preservative film It is overlying on container, to reduce moisture evaporation；Treat sowing 90 days or so, seedling tender stem by red turn it is green when, the seed being deposited on seedling is cut with the scissors sterilized, carry out Recovery；Fresh plant ash is smeared in the wound for having wiped out the seedling of seed, and is transplanted in time in nutritive cube；（5）Second of sowing：The seed of recovery is sowed, by step（3）Carry out；（6）Second batch seedling replanting：Seed to be reclaimed to sow 60 days or so, nursery stock sprouts and long to certain altitude substantially, now opens the container cover of constraint growth, It is overlying on preservative film on container, to reduce moisture evaporation；And optionally suitably keep the skin wet, river sand water content is maintained at 15% Left and right；Continue culture 120 days or so, seedling tender stem by red turn it is green when, the seed being deposited on seedling is cut with the scissors sterilized, Reclaimed again；Fresh plant ash is smeared in the wound for having wiped out the seedling of seed, and is transplanted to nutritive cube culture in time；（7）Seed soaking：The seed of secondary recovery is placed in clean basin, adds 500mg/L gibberellin treatment fluid, do not had a seed, under normal temperature Soaked seed 24h, and clear water is cleaned repeatedly；（8）Third time is sowed：Secondary recovery and the seed of seed soaking are sowed, by step（3）Carry out；（9）3rd batch of seedling replanting：After planting 90 days or so, the seed of secondary recovery was sprouted and grows to certain altitude, can be by seedling and the kind being deposited on seedling One piece of son is transplanted into nutritive cube culture.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108142266A (en) * | 2018-01-15 | 2018-06-12 | 浙江省林业科学研究院 | A kind of Ostrya rehderiana one year old seedling breeding method |
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