CN105144912B - A kind of method that largeflower-like honeysuckle flower seed is sprouted - Google Patents
A kind of method that largeflower-like honeysuckle flower seed is sprouted Download PDFInfo
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- CN105144912B CN105144912B CN201510667131.XA CN201510667131A CN105144912B CN 105144912 B CN105144912 B CN 105144912B CN 201510667131 A CN201510667131 A CN 201510667131A CN 105144912 B CN105144912 B CN 105144912B
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Abstract
The invention discloses a kind of method that largeflower-like honeysuckle flower seed is sprouted, complete full largeflower-like honeysuckle flower seed is specifically placed in 12~24h of immersion in 100~500 μ g/ml carbendazim or 500~l000 μ g/ml thiophanate methyl solution, after being rinsed with clear water, it is placed in the river sand of the sterilized processing of 4 DEG C of moistenings, covering more than 5cm sand bed carries out wet sand lamination 20~100 days；It is subsequently placed in illumination or dark sprouting under the conditions of 5~20 DEG C.The method of the present invention is simple, by controlling sterilization method, Stratification time, germination temperature and illumination, percentage of seedgermination is reached more than 60%, and the germination cycle is short, rate of going mouldy is low, and the accuracy rate of germination test is high, fundamentally solves the problem of largeflower-like honeysuckle flower dormancy period length, germination test difficulty.
Description
Technical field
The invention belongs to field of agricultural cultivation, and in particular to a kind of method that largeflower-like honeysuckle flower seed is sprouted.
Background technology
Largeflower-like honeysuckle flower (Lonicera macranthoides HAND.-Mazz.) is the perennial rattan of Caprifoliaceae Lonicera
This plant, its bud dried have the effect of clearing heat and detoxicating, cool dispersing wind and cold, recorded in the 2010 years version pharmacopeia Honeysuckle flowers in China
Under.Largeflower-like honeysuckle flower has the characteristics that yield is high, chlorogenic acid isoreactivity component content is high, annidation is strong, in Chongqing, lake
About 400 hectares of the ground such as south, Guizhou, Sichuan cultivated area, economy, ecological benefits highlight.
Traditional largeflower-like honeysuckle flower raising technology is based on the vegetative propagations such as cuttage, grafting, but in recent years, largeflower-like honeysuckle flower
Fine-variety breeding has larger progress, and cultivation breeding is planted up to more than 10, expands numerous breeding using graft technology in production.The reality of seminal propagation
Raw seedling has the characteristics of root system activity is strong, grafting survival rate is high, production cost is low, is widely used stock material.Natural bar
Under part, largeflower-like honeysuckle flower seed has the characteristic of dormancy, and generally requiring could sprout in soil by 5~6 months, and germinates
Rate only has 10% or so；Largeflower-like honeysuckle flower seed is tested using the germination test of routine, largeflower-like honeysuckle flower seed is not sent out
Bud, rate of going mouldy height.Therefore, break seed dormancy and the method for germination test there is an urgent need to a kind of, be that largeflower-like honeysuckle flower seed is numerous
Offer technical guarantee is provided.
The content of the invention
In view of this, it is an object of the invention to provide a kind of method that largeflower-like honeysuckle flower seed is sprouted, this method are simple
It is feasible, it can effectively solve the problem that the problems such as largeflower-like honeysuckle flower dormancy time is long, germination percentage is low, germination test is difficult.
For achieving the above object, the present invention provides following technical scheme：One kind breaks largeflower-like honeysuckle flower seed dormancy
With the method for germination test, complete full largeflower-like honeysuckle flower seed is placed in 100~500 μ g/ml carbendazim or 500~l000
12~24h is soaked in μ g/ml thiophanate methyl solution, after being rinsed with clear water, is placed in the river sand of the sterilized processing of 4 DEG C of moistenings
In, covering more than 5cm sand bed carries out wet sand lamination 20~100 days；Illumination or dark under the conditions of 5~20 DEG C is subsequently placed in sprout
Hair.
Preferably, the Stratification time of the wet sand lamination is at least 20 days.It is furthermore preferred that during the lamination of the wet sand lamination
Between be 20~100 days.Most preferably, the Stratification time of the wet sand lamination is 80 days.
, can effectively to solve the germination percentage of largeflower-like honeysuckle flower seed under field conditions (factors) extremely low for breaking dormancy by cool stratification
The problem of, lamination 80d seed beginning germinating times are minimum, are 3.00d, germination percentage highest, up to 71.69%, germinating energy highest；Lamination
Amount of activated big seed has begun to germinate in 4 DEG C of wet sands after 80d.
It has been investigated that germinating bed can not normally germinate between soil of the seed more than earthing 1cm, on paper, between paper, it is husky
It can be germinateed between upper, husky or on the upper 5 kinds of germinating beds of soil, to be germinateed on paper and on sand, index is optimal, and optimum is as germination test
Germinating bed, the accumulation of Seedling Biomass is most appropriate on sand.Therefore the germinating bed that the present invention preferably sprouts is that germinating bed is paper
Between upper, paper, in Sha Shang, sand or on soil；On more preferably husky upper or paper.The germinating bed of the present invention is by country of the People's Republic of China (PRC)
Standard GB/T3543.4-1995 prepares.
In the present invention, largeflower-like honeysuckle flower seed after the Stratificated treatment seed under the conditions of 5~20 DEG C can germinate, but 15
~20 DEG C of seed seedling could the normal development in 30d, percentage of seedgermination, germinating energy and germination index be most under 15 DEG C of illumination conditions
Height, respectively 60.00%, 44.17% and 3.97%.Therefore, the condition that the present invention preferably sprouts is the illumination at 15~20 DEG C
Or dark sprouting, more preferably it is illumination or dark sprouting at 15 DEG C, is most preferably sprouted in 15 DEG C of illumination.
The beneficial effects of the present invention are：The method that a kind of largeflower-like honeysuckle flower seed disclosed by the invention is sprouted, its method
Simply, seed quick-speed germination is promoted by cool stratification, improves seed germination rate, shortened seed sprout time, be then selected in conjunction
Suitable sprouting condition and germinating bed, the germination percentage, germinating energy and germination index of seed is reached optimum state, while reduce seed
Rate of going mouldy, germination potentiality can be made to reach maximum, disclosure satisfy that largeflower-like honeysuckle flower seed seedling-raising in production, had good
Market prospects.
Brief description of the drawings
In order that the purpose of the present invention, technical scheme and beneficial effect are clearer, the present invention provides drawings described below：
Fig. 1 is the germination percentage of Different hypothermia Stratification time largeflower-like honeysuckle flower seed.
Embodiment
Below in conjunction with accompanying drawing, the preferred embodiments of the present invention are described in detail.It is unreceipted specific in embodiment
The experimental method of condition, generally according to normal condition or according to the condition proposed by manufacturer.
Experiment material：The largeflower-like honeysuckle flower seed that the present invention uses picks up from Chongqing City Jiangjin District Berlin town in November, 2014
Dong Sheng villages largeflower-like honeysuckle flower planting base.Gained seed is broken to be rubbed after ripe berry harvesting, after water breaks through the residues such as filter pericarp, from
So air-dry and obtain, be identified as largeflower-like honeysuckle flower L.macranthoides seeds.
1st, largeflower-like honeysuckle flower seed is sprouted
Complete full largeflower-like honeysuckle flower seed is placed in 12~24h of immersion in 100~500 μ g/ml carbendazim solutions, used
Clear water is placed in after rinsing in the river sand of the sterilized processing of 4 DEG C of moistenings, covering more than 5cm sand bed carry out wet sand lamination 20~
100 days；It is subsequently placed in illumination or dark sprouting under the conditions of 5~20 DEG C.
2nd, the influence that seed disinfection is sprouted to seed
From 3 kinds of triazolone, carbendazim and thiophanate methyl chemical pesticides, and 0 μ g/ml, 100 μ g/ml are each configured to,
500 μ g/ml, the seed disinfection solution of 1000 concentration of μ g/ml 4, complete full largeflower-like honeysuckle flower seed is respectively placed in and disappeared
22h is soaked in venom, after being rinsed with clear water, is respectively charged into canvas pocket, is placed in the river sand of the sterilized processing of 4 DEG C of moistenings
In, lamination 80d.The seed of Stratificated treatment is taken, is placed in 15 DEG C of (illumination 8h+ dark 16h) incubators, is entered with germinating bed on paper
Row germination test, 100 seeds are often handled, are repeated 3 times.After 14 days, statistics percentage of seedgermination and rate of going mouldy, 1 the results are shown in Table.
The influence of table 1, different agents seed disinfection to largeflower-like honeysuckle flower percentage of seedgermination and rate of going mouldy
As shown in Table 1, after carbendazim and thiophanate methyl seed disinfection, the rate of going mouldy significantly reduces, wherein 500 μ g/ml are more
The rate of going mouldy of bacterium spirit solution processing is minimum, is 10.87%.1000 μ g/ml triazolones solution and 1000 μ g/ml carbendazim solution kinds
After son sterilization, germination percentage significantly reduces.Therefore, suggest using 100~500 μ g/ml carbendazim and 500~l000 μ g/ in production
Ml thiophanate methyl solution, 12-24h is soaked, complete seed disinfection.
3rd, the influence that earthing of seeds processing is sprouted to seed
Whole full seed is taken per 20d during lamination, is placed in 15 DEG C of (illumination 8h+ dark 16h) incubators, with paper
Germinating bed carries out germination test, often handles 100 seeds, is repeated 3 times.Record germination daily, until there is no new kind
Son germination, as a result as shown in Figure 1.As shown in Figure 1, within the specific limits, as Stratification time increases the contracting of seed beginning germinating time
Short, germination percentage and germinating energy improve.Non- lamination seed beginning germinating time is 30.33d, germination percentage 16.20%, and germination is bent
Line slope is minimum, i.e., germinating energy is minimum；Lamination 80d seed beginning germinating times are minimum, up to 3.00d, germination percentage highest, reach
71.69%, percentage of seedgermination slope of curve highest, germinating energy highest；When lamination 100d seed beginnings germinating time is no longer with lamination
Between increase and increase, germination percentage is reduced to 56.61%, and the percentage of seedgermination slope of curve is with lamination 80d seeds without significant difference.Knot
Fruit shows, cool stratification can effective breaking dormancy, improve seed and sprout.
4th, the influence that illumination and Temperature Treatment are sprouted to seed
5,10,15,20,25 DEG C of constant temperatures are set, and each temperature conditionss set illumination (illumination 8h+ dark 16h) and black
Dark two processing, totally 10 processing.Each processing takes 4 DEG C of lamination 80d 100, seed to be placed on 3 layers of moistening filter paper, repeats 4
It is secondary.Observe daily and record germinative number, as a result as shown in table 2.
Table 2, different temperatures and illumination to largeflower-like honeysuckle flower germination influence (N=4)
As shown in Table 2, different temperatures influences notable on the indices of germination, and mutually synthermal different photo-irradiation treatments
Each index of lower germination is without significant difference, and on the whole, seed can germinate under 5~20 DEG C of illumination and dark condition, and 25
DEG C illumination and dark condition seed do not germinate.
Seed beginning germinating time is raised and reduced with temperature, and 20 DEG C of illumination condition lower beginning germinating times are minimum, are 3.50d,
And the seed beginning germinating time of 15 DEG C of temperature groups and 20 DEG C of temperature groups is without significant difference.It is minimum at 5 DEG C of germination duration,
Highest at 10 DEG C.Percentage of seedgermination, germinating energy and germination index highest under 15 DEG C of illumination conditions, respectively 60.00%,
44.17% and 3.97, the percentages of seedgermination of 5 DEG C of temperature groups, germinating energy and germination index are minimum, the germination of 10 DEG C of temperature groups
Without significant difference, the potentiality of seed and germination index of 10 DEG C of temperature groups are substantially less than 20 DEG C of temperature groups for rate and 20 DEG C of temperature groups
's.
The influence of temperature and illumination condition to the rate of going mouldy is without significant rule, under 5 DEG C of illumination and dark condition, 10 DEG C of illumination
Under the conditions of, rate of going mouldy under 15 DEG C of illumination and dark condition is minimum has no significant difference, respectively 6.25%, 6.25%, 9.17%,
10.83% and 10.00%.
Then seed puts a 30d, and 10 representational germination seed graph paper methods are randomly selected from each processing
Radicle length, the length and width of cotyledon of seed seedling are determined, weighs seedling fresh weight, as a result as shown in table 3.
Under table 3, different temperatures and illumination condition seed seedling development index (N=4)
As shown in Table 3,10 DEG C of temperature group seed base-roots increase, but plant skin and do not fall off, and cotyledon is un-extended.15 DEG C, 20 DEG C of temperature
The equal normal development of degree group seed seedling, but radicle is long, cotyledon is long and cotyledon is wide significant difference be present.Seed under 15 DEG C of illumination conditions
Seedling development is optimal, and radicle is long, cotyledon is long and cotyledon is wide reaches maximum, respectively 20.93mm, 4.89mm, 3.15mm.15℃
Seed seedling cotyledon is wide under the wide noticeably greater than dark condition of seed seedling cotyledon under illumination condition, remaining mutually synthermal different illumination
Condition seed seedling development difference is not notable.
5th, the influence that germinating bed processing is sprouted to seed
Set on paper, between paper, on sand, between sand, on soil, 6 kinds of germinating beds processing between soil, wherein covered between sand between soil
Lid thickness reaches 1cm.Each processing takes 4 DEG C of lamination 80d 100, seed, is repeated 4 times, most suitable illumination and temperature in 1.2.4 screenings
Degree is lower to carry out germination test, observes daily and records germinative number and mildew and rot seed number, as a result as shown in table 4.
Table 4, different germinating beds to largeflower-like honeysuckle flower germination influence (N=4)
Note：Different letters represent the significance level of difference (P in same row<0.05)
As shown in Table 4, for largeflower-like honeysuckle flower seed under 15 DEG C of illumination conditions, seed can not be normal between earthing 1cm soil
Germinate, can be germinateed on other 5 kinds of germinating beds.The seed beginning, germinating time was without significant difference on 5 kinds of germinating beds；Seed hair on soil
The bud duration is most short, be on 16.00d, with paper, Sha Shang, it is husky between and the upper germinating bed of soil seed without significant difference；Kind on sand
Sub- germination percentage highest, between 64.17%, with paper, Sha Shang, soil on without significant difference；Germinating energy and germination index highest on paper,
For on 44.17% and 4.17, with sand, soil on without marked difference；Rate of being gone mouldy on soil highest, it is 16.67%.Comprehensive germination index,
On paper and sand goes up germinating bed of the optimum as largeflower-like honeysuckle flower seed germination experiment.
Then seed seedling quality is determined, as a result as shown in table 5.
Table 5, different germinating bed seed seedling development index (N=4)
Note：Significance difference analysis takes=0.05 horizontal, different letter expression significant differences in same row.
As shown in Table 5, the long difference of seed seedling radicle on paper between paper is not notable, respectively 20.93mm and
21.47mm, it is significantly higher than the seed seedling radicle length of other three kinds of germinating beds.Seed seedling cotyledon length and cotyledon on paper is wide most
Greatly, the seed seedling respectively on 4.89mm and 3.15mm, with sand, on soil is significantly higher than between paper between sand without significant difference
Seed seedling.Seed seedling weight highest on sand, up to 0.027g, the seed seedling weight being significantly higher than between sand.In general, on paper
Most beneficial for the growth of seed base-root between paper, the accumulation of Seedling Biomass is most appropriate on sand, is unfavorable for son between sand between paper
The stretching, extension of leaf.
Summary result of study, the standard germination testing regulations of largeflower-like honeysuckle flower are：4 DEG C of wet sand lamination 80d of seed, paper
Germinating bed on upper or sand, 15 DEG C of constant temperature illumination cultivations.
Finally illustrate, preferred embodiment above is merely illustrative of the technical solution of the present invention and unrestricted, although logical
Cross above preferred embodiment the present invention is described in detail, it is to be understood by those skilled in the art that can be
Various changes are made to it in form and in details, without departing from claims of the present invention limited range.
Claims (3)
1. a kind of method that largeflower-like honeysuckle flower seed is sprouted, it is characterised in that：Complete full largeflower-like honeysuckle flower seed is placed in
12 ~ 24h is soaked in 100 ~ 500 μ g/ml carbendazim or 500 ~ l000 μ g/ml thiophanate methyl solution, 4 are placed in after being rinsed with clear water
In the river sand of the sterilized processing of DEG C moistening, 5 more than cm of covering sand bed carries out wet sand lamination 80 days；It is subsequently placed in 5 ~ 20
Illumination or dark sprouting under the conditions of DEG C；The germinating bed of the sprouting is on paper, between paper, in Sha Shang, sand or on soil.
2. a kind of method that largeflower-like honeysuckle flower seed is sprouted according to claim 1, it is characterised in that：The hair of the rudiment bed
Bud bed is on husky upper or paper.
3. a kind of method that largeflower-like honeysuckle flower seed is sprouted according to claim 1, it is characterised in that：The condition of the sprouting
It is illumination or dark sprouting at 15 DEG C.
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| CN106561112A (en) * | 2016-11-08 | 2017-04-19 | 绥阳县吉帮昌盛金银花农业科技有限公司 | Floating seedling culture method for honeysuckle |
| CN113545196A (en) * | 2021-09-07 | 2021-10-26 | 绥阳县吉帮昌盛金银花农业科技有限公司 | Storage method for improving germination rate of lonicera confusa |
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| CN103843566A (en) * | 2014-03-07 | 2014-06-11 | 王贵琴 | Honeysuckle planting method |
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