CN113545196A - Storage method for improving germination rate of lonicera confusa - Google Patents
Storage method for improving germination rate of lonicera confusa Download PDFInfo
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- CN113545196A CN113545196A CN202111045031.5A CN202111045031A CN113545196A CN 113545196 A CN113545196 A CN 113545196A CN 202111045031 A CN202111045031 A CN 202111045031A CN 113545196 A CN113545196 A CN 113545196A
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- seeds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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Abstract
The invention provides a storage method for improving the germination rate of lonicera confusa, which comprises the steps of pretreating lonicera confusa seeds, adding warm water into gleditsia sinensis for soaking and degreasing, then sterilizing by gibberellin, placing the lonicera confusa seeds in a multilayer seed tray in a cellar, adopting mixed soil layers for stacking storage at low temperature, and carrying out variable-temperature germination acceleration before the lonicera confusa seeds are taken out of the cellar. According to the lonicera confusa storage method, the energy consumption of storage management is reduced by utilizing the characteristics of the cellar, the seeds are stored and the seeds are broken to sleep, the germination rate of the lonicera confusa seeds is improved, the germination time is shortened, and the planting cost of the lonicera confusa is greatly reduced.
Description
Technical Field
The invention belongs to the field of lonicera confusa planting, and particularly relates to a lonicera confusa seed storage method.
Background
The Lonicera confusa is dried bud or flower with initial blossom of Lonicera macranthoides, Lonicera hypoglauca, Lonicera confusa or Lonicera fulvidraco of Caprifoliaceae. Live in creeks, open fields, under forests or in shrubs. Produced in all parts of the south of China. Nature, flavor and meridian tropism: sweet and cold in nature. It enters lung, heart and stomach. The research of Von warming of doctor who is doctor of plant research institute of Jiangsu province of Chinese academy of sciences discovers a large amount of triterpenoid soap components in the chemical components of lonicera macranthoides, and the components have the activities of protecting liver and resisting cancer. The first professor of Guangzhou university of traditional Chinese medicine and doctor, who is Xunhua, shows that many experts in the medical field are seeking traditional Chinese medicines to replace western antibiotics when examining the demonstration base of Lonicera macranthoides in the Zhanjiang province of Guangdong, and Lonicera macranthoides is an ideal substitute (because of high chlorogenic acid content, the average content is up to more than 6.196%), and can reduce the side effect of the medicines on human bodies. Therefore, if the Lonicera macranthoides is used for producing the traditional Chinese medicine for replacing antibiotics in the early days, the market prospect is immeasurable.
However, the lonicera confusa seeds have the characteristic of deep dormancy, so that the germination rate is only about 10% after sowing, the planting cost is increased, and the popularization of planting is influenced.
Disclosure of Invention
The invention aims to provide a method for improving the germination rate of lonicera confusa, which achieves the double effects of storage and dormancy breaking by pretreating lonicera confusa seeds and storing the lonicera confusa seeds in a cellar.
The technical scheme of the invention is as follows:
a storage method for improving the germination rate of lonicera confusa comprises the following steps:
a. pretreating flos Lonicerae seed, soaking in warm water and fructus Gleditsiae Abnormalis water for 48 hr, defatting, drying in the shade, and sterilizing with disinfectant;
b. arranging a plurality of rows of placing frames in the cellar, wherein the placing frames can be used for placing a plurality of layers of seed trays, and the layer interval is 30-50 cm;
c. b, transferring the lonicera confusa seeds treated in the step a into a seed tray in a cellar, laminating the seeds by adopting a mixed soil layer, keeping the thickness of the soil layer to be 10-15 cm, keeping the mixed soil layer moist until the mixed soil layer can be naturally dispersed without yielding water, keeping the temperature in the cellar to be-5 ℃, and ensuring the humidity of the mixed soil layer during storage;
d. before sowing, the temperature in cellar is regulated to 15-25 deg.C and kept for 10 days, then the temperature is reduced to 5-15 deg.C and kept for 10 days, and then temp. -changing germination acceleration is implemented.
Preferably, the temperature of the warm water in step a is 35 ℃.
Preferably, the disinfectant in the step a is gibberellin with the concentration of 50-150 mg/L.
Preferably, the gibberellin concentration in step a is 120 mg/L.
Preferably, the seed tray in the step a is provided with air holes.
Preferably, the soil mixture layer in the step c is sandy soil and vermiculite.
Detailed Description
The present invention will be described in further detail with reference to specific examples.
It should be understood that the terms "upper", "lower", "left", "right", "middle", and the like used herein are for descriptive convenience only and are not intended to limit the scope of the invention, and that changes and modifications in the relative relationship thereof are also considered to be within the scope of the invention without substantial technical changes.
The lonicera confusa seeds are long in sleep breaking time and low in budding rate, if sleep breaking treatment is only carried out before planting, long time can be consumed, the lonicera confusa seed storage time and the sleep breaking time are combined, and the time consumed by planting production management can be reduced.
After the mature lonicera confusa seeds are collected, the collected seeds are usually soaked for a certain time, then the pulp is separated by adopting a foot stepping mode and the like, and the seeds are left for air drying and then are stored.
The method for storing the lonicera confusa seeds comprises the following steps of:
a. pretreating flos Lonicerae seed, soaking in warm water and fructus Gleditsiae Abnormalis water for 48 hr, defatting, drying in the shade, and sterilizing with disinfectant;
b. arranging a plurality of rows of placing frames in the cellar, wherein the placing frames can be used for placing a plurality of layers of seed trays, and the layer interval is 30-50 cm;
c. b, transferring the lonicera confusa seeds treated in the step a into a seed tray in a cellar, laminating the seeds by adopting a mixed soil layer, keeping the thickness of the soil layer to be 10-15 cm, keeping the mixed soil layer moist until the mixed soil layer can be naturally dispersed without yielding water, keeping the temperature in the cellar to be-5 ℃, and ensuring the humidity of the mixed soil layer during storage;
d. before sowing, the temperature in cellar is regulated to 15-25 deg.C and kept for 10 days, then the temperature is reduced to 5-15 deg.C and kept for 10 days, and then temp. -changing germination acceleration is implemented.
In the step a, the adopted warm water is 35 ℃, the adopted disinfectant is gibberellin with the concentration of 50-150 mg/L, the activity of the seeds can be better excited when the water temperature is 35 ℃, and the germination rate of the seeds can be further improved by matching with the catalytic action of the gibberellin.
In order to better stimulate the activity of the seeds, a mixed soil layer formed by mixing sandy soil and vermiculite is adopted in the step c, and the characteristics of capability of loosening soil, good air permeability, strong water absorption, small temperature change and the like of the vermiculite are utilized, so that the required management and maintenance time can be reduced in the preservation process, and the seeds can be effectively prevented from mildewing or the mixed soil layer has excessive water. And in the planting of later stage, also can reduce the waste material and drop into as soil nutrition replenishment, for further promoting the gas permeability of mixing the soil layer, can set up the bleeder vent of certain figure on the dish.
The invention has the following embodiments:
the first embodiment is as follows:
the method for storing the lonicera confusa seeds comprises the following steps:
a. pretreating flos Lonicerae seed, soaking in 35 deg.C warm water and fructus Gleditsiae Abnormalis water for 48 hr to remove fat, drying in the shade, and sterilizing with 50mg/L gibberellin;
b. arranging a plurality of rows of placing frames in the cellar, wherein the placing frames can be used for placing a plurality of layers of seed trays, and the layer interval is 30 cm;
c. b, transferring the lonicera confusa seeds treated in the step a into a seed tray in a cellar, wherein the seeds are laminated by adopting a mixed soil layer, the thickness of the soil layer is 10 cm, the mixed soil layer is kept moist, the natural dispersion of water cannot occur, the temperature in the cellar is 5 ℃, and the humidity of the mixed soil layer is ensured during storage;
d. the temperature in the cellar is adjusted to 20 ℃ and kept for 10 days before sowing, and then the temperature is reduced to 10 ℃ and kept for 10 days.
Example two:
the method for storing the lonicera confusa seeds comprises the following steps:
a. pretreating flos Lonicerae seed, soaking in 35 deg.C warm water and fructus Gleditsiae Abnormalis water for 48 hr to remove fat, drying in the shade, and sterilizing with 150mg/L gibberellin;
b. arranging a plurality of rows of placing frames in the cellar, wherein the placing frames can be used for placing a plurality of layers of seed trays, and the layer interval is 50 cm;
c. b, transferring the lonicera confusa seeds treated in the step a into a seed tray in a cellar, wherein the seeds are laminated by adopting a mixed soil layer, the thickness of the soil layer is 15 cm, the mixed soil layer is kept moist, the mixed soil layer is naturally dispersed without water, the temperature in the cellar is-5 ℃, and the humidity of the mixed soil layer is ensured during storage;
d. the temperature in the cellar is adjusted to 25 ℃ and kept for 10 days before sowing, and then the temperature is reduced to 5 ℃ and kept for 10 days.
Example three:
the method for storing the lonicera confusa seeds comprises the following steps:
a. pretreating flos Lonicerae seed, soaking in 35 deg.C warm water and fructus Gleditsiae Abnormalis water for 48 hr to remove fat, drying in the shade, and sterilizing with 120mg/L gibberellin;
b. arranging a plurality of rows of placing frames in the cellar, wherein the placing frames can be used for placing a plurality of layers of seed trays, and the interlayer spacing is 40 cm;
c. b, transferring the lonicera confusa seeds treated in the step a into a seed tray in a cellar, wherein the seeds are laminated by adopting a mixed soil layer, the thickness of the soil layer is 10 cm, the mixed soil layer is kept moist, the natural dispersion of water cannot occur, the temperature in the cellar is 1 ℃, and the humidity of the mixed soil layer is ensured during storage;
d. the temperature in the cellar is adjusted to 20 ℃ and kept for 10 days before sowing, and then the temperature is reduced to 5 ℃ and kept for 10 days.
In the process of disinfecting seeds by using gibberellin, the effect of breaking the seeds by the gibberellin is gradually increased along with the concentration, when the concentration reaches 100mg/L, the effect is gradually reduced, the improvement is not obvious, and 120mg/L of gibberellin is used as the best choice.
Before sowing, the seeds are stimulated by changing the temperature in the cellar, and the temperature is raised to 15-25 ℃ and then lowered to 5-15 ℃ by two-stage temperature change, so that the germination time of the seeds is shortened.
The lonicera confusa seed storage method adopted by the invention can break the seeds to sleep while storing the lonicera confusa seeds, and when the seeds need to be planted, the planting operation can be carried out only by carrying out temperature change treatment in advance; by utilizing the characteristic of good heat preservation performance of the cellar, the management energy consumption during seed storage is reduced, and the temperature is kept constant. The germination rate of the lonicera confusa seeds stored by the method is greatly improved and is close to 60%, the germination time is reduced by 10-15 days, and the planting efficiency is greatly improved.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (6)
1. A storage method for improving the germination rate of lonicera confusa is characterized by comprising the following steps:
a. pretreating flos Lonicerae seed, soaking in warm water and fructus Gleditsiae Abnormalis water for 48 hr, defatting, drying in the shade, and sterilizing with disinfectant;
b. arranging a plurality of rows of placing frames in the cellar, wherein the placing frames can be used for placing a plurality of layers of seed trays, and the layer interval is 30-50 cm;
c. b, transferring the lonicera confusa seeds treated in the step a into a seed tray in a cellar, laminating the seeds by adopting a mixed soil layer, keeping the thickness of the soil layer to be 10-15 cm, keeping the mixed soil layer moist until the mixed soil layer can be naturally dispersed without yielding water, keeping the temperature in the cellar to be-5 ℃, and ensuring the humidity of the mixed soil layer during storage;
d. before sowing, the temperature in cellar is regulated to 15-25 deg.C and kept for 10 days, then the temperature is reduced to 5-15 deg.C and kept for 10 days, and then temp. -changing germination acceleration is implemented.
2. The storage method according to claim 1, wherein: the temperature of the warm water in the step a is 35 ℃.
3. The storage method according to claim 1, wherein: the disinfectant in the step a is gibberellin with the concentration of 50-150 mg/L.
4. A method of storing as claimed in claim 3, wherein: the concentration of the gibberellin in the step a is 120 mg/L.
5. The storage method according to claim 1, wherein: and a seed tray in the step a is provided with air holes.
6. The storage method according to claim 1, wherein: and in the step c, the mixed soil layer is sandy soil and vermiculite.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202111045031.5A CN113545196A (en) | 2021-09-07 | 2021-09-07 | Storage method for improving germination rate of lonicera confusa |
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| CN202111045031.5A CN113545196A (en) | 2021-09-07 | 2021-09-07 | Storage method for improving germination rate of lonicera confusa |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114568135A (en) * | 2022-03-25 | 2022-06-03 | 成都中医药大学 | Preservation method and germination method of evergreen fruit tree seeds |
Citations (4)
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|---|---|---|---|---|
| FR2577595A1 (en) * | 1985-02-19 | 1986-08-22 | Continentale Chimitec Sarl | Prefabricated garden cellar |
| CN105144912A (en) * | 2015-10-15 | 2015-12-16 | 重庆市中药研究院 | Method for germination of lonicera macranthoides seeds |
| CN105230315A (en) * | 2015-11-06 | 2016-01-13 | 广西大海阳光药业有限公司 | Rapid flos lonicerae propagation method |
| CN107333482A (en) * | 2016-11-03 | 2017-11-10 | 黑龙江省科学院自然与生态研究所 | The processing method that a kind of northeast maple seed Dormancy breaking is emerged then |
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2021
- 2021-09-07 CN CN202111045031.5A patent/CN113545196A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2577595A1 (en) * | 1985-02-19 | 1986-08-22 | Continentale Chimitec Sarl | Prefabricated garden cellar |
| CN105144912A (en) * | 2015-10-15 | 2015-12-16 | 重庆市中药研究院 | Method for germination of lonicera macranthoides seeds |
| CN105230315A (en) * | 2015-11-06 | 2016-01-13 | 广西大海阳光药业有限公司 | Rapid flos lonicerae propagation method |
| CN107333482A (en) * | 2016-11-03 | 2017-11-10 | 黑龙江省科学院自然与生态研究所 | The processing method that a kind of northeast maple seed Dormancy breaking is emerged then |
Non-Patent Citations (3)
| Title |
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| 孟泉科: "金银忍冬种子变温催芽处理的研究", 《安徽农业科学》 * |
| 河南林业局 编: "《造林技术课本》", 31 July 1956, 河南人民出版社 * |
| 胡尚欣 等: "《中药材栽培技术》", 31 May 2009, 四川教育出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114568135A (en) * | 2022-03-25 | 2022-06-03 | 成都中医药大学 | Preservation method and germination method of evergreen fruit tree seeds |
| CN114568135B (en) * | 2022-03-25 | 2023-10-20 | 成都中医药大学 | Preservation method and germination method of evergreen fruit tree seeds |
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