CN101199251A - Leymus chinensis seeds palea removing and temperature changing combination germinating method - Google Patents
Leymus chinensis seeds palea removing and temperature changing combination germinating method Download PDFInfo
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- CN101199251A CN101199251A CNA2006101632451A CN200610163245A CN101199251A CN 101199251 A CN101199251 A CN 101199251A CN A2006101632451 A CNA2006101632451 A CN A2006101632451A CN 200610163245 A CN200610163245 A CN 200610163245A CN 101199251 A CN101199251 A CN 101199251A
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Abstract
The invention is a chinensis germination method of combined seed peeling and temperature changing, which belongs to the planting technique field. The invention mainly comprises two parts of: partially or wholly removing the hull of chinensis seed by means of hull peeling so as to break the hard physical structure of the hull and releasing the chinensis seed dormancy aiming to destroy the hard phyical structure of the hull and break the chinensis seed dormancy; as well as rapidly increasing the seed germination rate of chinensis by using the variable temperature technique. The invention concretely has four processes of 1) seed selection and impurity removal, 2) hull-peeling treatment, 3) seed disinfection and 4) variable temperature germination. The invention has the increased seed germination rate from 54.0 percent to over 95.0 percent compared with the seed with hull. Therefore, the invention has significant practical meaning and broad application value to both the chinensis seed cultivation and experimental study.
Description
Technical field
The invention belongs to the plant planting technical field, relate to the germinating method of plant seed breaking dormancy, specifically break the severe dormancy of Leymus chinensis seeds fast, significantly improve the combined method of Leymus chinensis seeds germination rate.
Background technology
Sheep's hay (Leymus chinensis) is the perennial rhizome plant of grass family leymus, is a kind of high quality forage.This herbage mainly is distributed in the eastern region on Eurasian prairie, comprises Muscovite outer Baikal, the east of Mongolia and the north, northeast song-Nen plain, the North China Plain and the Nei Mongol Plateau area of China.Sheep's hay has the very high economic worth and the ecological value, is rich in high-quality protein, mineral matter and carbohydrate.Its fast growth, drought-resistant saline and alkaline and dead soil.Yet, in recent years because the overgraze and the reclamation of wasteland cause original a large amount of high-quality sheep's hays meadow to become the saline and alkaline desert that has no value.Therefore, how to improve degenerated soil, enlarge the task of top priority (Liang Zhengwei etc., 2004, the method for soda alkaline land sheep's hay vegetation restoration and reconstruction, number of patent application 200410010867.1) that the sheep's hay cultivated area has become production and scientific research fast.
Leymus chinensis seeds has the unique biological characteristic, and resting stage reaches more than the 4-5.New seed generally only has the low germination rate of 10-20%.One of main bottleneck technology that present plantation sheep's hay faces is severe dormancy how to remove Leymus chinensis seeds fast, significantly improves the problem of percentage of seedgermination.Former studies shows that Leymus chinensis seeds germination rate under 25-26 ℃ constant temperature is very low.Germination rate when not adding any medicine and handle only is 1.8%; Use 1%HNO
3The germination rate of handling is 8%.In addition, in the soil germinating bed of differing texture, chernozem germination rate the highest (42.8%), clay take second place (27.9%), and sand is 20.2%.Yi Jin (dormant physiology of Leymus chinensis seeds and the research that improves germination rate, the China meadow, 1994, (6): 1-6) Leymus chinensis seeds after will gathering in the crops places dry storage under the indoor 15-25 ℃ normal temperature, carrying out germination rate by the seed to storage 1-8 at 20 ℃ of constant temperatures measures, find paper training method to preserve the minimum of 1 year and 8 years, germination rate only is 1%; Preserve the highest of 4 years and 5 years, but germination rate also has only 5%; Sha Peifa is to preserve 1 year minimum, and germination rate is 7%, and to preserve 6 years the highest, germination rate is 45%; The earth culture method with preserve 1 year be 36%, the germination rate of preserving 6 years can bring up to 73%, but the seed storage overlong time is unfavorable for production application.
In sum, the germinating method of in the past breaking the Leymus chinensis seeds dormancy mainly is simple physics method and chemical method.Promptly 1) physical method mainly comprises simple constant temperature, alternating temperature, and handle in magnetic field, the germinating bed of differing texture (as earth culture, Sha Pei or paper training).2) chemical method has the rare earth seed soaking to handle, exogenous hormone processing etc., and (the just big Chen Yuan of Ma Hongyuan beam improves Leymus chinensis seeds germination rate method progress but action effect is all undesirable.The China meadow, in July, 2005,27 (4): 64-68).
The common drawback of these methods was in the past: 1) lack a unified standard germinating method, comparativity is relatively poor between the data.2) be better than the fact that constant temperature germinates though found the alternating temperature germination, do not find desirable alternating temperature amplitude of a cover and method combination all the time.3) seed that does not have to find to break simply a Leymus chinensis seeds dormancy is arranged (impurity elimination+seed soaking etc.) technology in advance.What 4) adopt mainly is single processing method, arrange the in advance technology and the alternating temperature organic assembling of seed is not got up to create new germinating method and technology.
Summary of the invention
The purpose of this invention is to provide Leymus chinensis seeds palea removing and temperature changing combination germinating method.
The present invention is in order to improve the Leymus chinensis seeds germination rate by arranging the control of technology and temperature to combine in advance, create a kind of combination germinating method, its core mainly comprises two parts, promptly 1) go bran to handle: to arrange in advance with seed and go the bran method with the bran skin portion of Leymus chinensis seeds or all remove in the technology, purpose is to destroy the firm physical arrangement of bran skin, removes the Leymus chinensis seeds dormancy.2) germinating method: mainly utilize the alternating temperature technology to improve the Leymus chinensis seeds germination rate fast.Both ingenious combinations have effectively breaks the dormancy of Leymus chinensis seeds severe fast, significantly improves the effect of Leymus chinensis seeds germination rate.
The present invention will arrange technology and temperature control to combine in advance, improve the Leymus chinensis seeds germination rate, specifically realize by following process.
1. seed cleaning impurity elimination
Because the Leymus chinensis seeds grain is little, and ripening rate is lower, the Leymus chinensis seeds after the results generally contains a large amount of impurity, therefore, guarantees that seed purity and cleanliness are to improve the key of Leymus chinensis seeds germination rate.Selected impurity elimination can adopt mechanical or manual method to carry out.Seed purity and cleanliness should reach more than 90%.
2. go bran to handle
Leymus chinensis seeds has the bran skin hard, that quality is fine and close usually, and water penetration, gas permeability are relatively poor, and the mechanical-physical obstacle of bran skin has a strong impact on the suction and the germination of Leymus chinensis seeds.Therefore, utilizing medicament, manual or mechanical processing method is clever shell with the bran skin of Leymus chinensis seeds, partly or entirely removes or peels off, and purpose is to destroy the firm physical arrangement of bran skin, can significantly improve the sheep's hay germination rate.Like this, go the seed process of bran processing or just can directly enter sterilization and germination program through above-mentioned without low temperature or high-temperature seed presoaking stage.
3. seed disinfection
Take out the above-mentioned seed of arranging in advance and promptly go the bran seeds treated, put into seed disinfectant and carried out surface disinfection 3-7 minute, thoroughly clean 3-4 time with clean water then and carry out seed sprouting again.Described seed disinfectant can be 0.1% mercuric chloride solution.
4. alternating temperature germinates
Temperature is the important factor that influences seed sprouting.Generally speaking, suitable temperature environment promotes seed germination, the too high or too low seed germination that all suppresses.For some species, as sheep's hay, even the constant temperature that suits can not be sprouted smoothly, and reasonably the alternating temperature combination can solve this germination difficult problem preferably.
The Leymus chinensis seeds of sterilizing is seeded in the various germinating beds, and as soil, filter paper is in the sand germinating bed etc.Be placed on low temperature 5-16 ℃, dark is under the situation of unglazed photograph, 12-16 hour; High temperature 28-35 ℃, under the light conditions, 12-8 hour.The time of low temperature, dark time and high temperature, illumination was to circulate in the cycle with 24 hours, under this alternating temperature condition germination 7-21 days.
The present invention can make the sheep's hay germination rate be brought up to more than 95.0% by 54.0% by the bran percentage of seedgermination that promptly has of contrast.Therefore, the present invention all has important practice significance and value widely to Leymus chinensis seeds cultivation and experimental study.
Embodiment
Embodiment
1. seed cleaning impurity elimination
For improving the Leymus chinensis seeds germination rate, method is by hand carried out selected impurity elimination, and seed purity and cleanliness should be reached more than 90%.
2. go bran to handle
Method by hand is that clever shell is peeled off with the bran skin of Leymus chinensis seeds, peels the seed of bran skin off, in order to carrying out seed disinfection.
3. seed disinfection
Take out the above-mentioned bran seeds treated of going, put into 0.1% mercuric chloride solution and carried out surface disinfection 5 minutes, thoroughly clean with clean water then and carry out seed sprouting again 4 times.
4. alternating temperature germinates
The Leymus chinensis seeds of sterilizing is seeded on the sand germinating bed, is positioned over 5 ℃ of low temperature, 5 ℃, 16 ℃, dark is under the situation of unglazed photograph, 14 hours; 35 ℃, 28 ℃, 28 ℃ of high temperature, under the light conditions, 10 hours.Under this alternating temperature condition, be to circulate in the cycle with 24 hours, germinateed 21 days.35 ℃, 28 ℃, 28 ℃ of 5 ℃, 5 ℃, 16 ℃ corresponding high temperature of difference of low temperature that wherein above-mentioned alternating temperature germinates are expressed as 5/35 ℃, 5/28 ℃, 16/28 ℃ in table.
By embodiment, the experimental result that obtains is as shown in table 3.All than under the same terms bran percentage of seedgermination height being arranged, and along with the increase of alternating temperature amplitude, germination rate also is remarkable increase trend to the Leymus chinensis seeds that removes bran under any three kinds of alternating temperature conditions.Go the high germination rate of bran seed to appear under 5/35 ℃ of alternating temperature condition, repeat up to 100% average out to 95.0% individually.
Table 3 removes bran and the main germination index of bran Leymus chinensis seeds under three kinds of alternating temperature amplitudes is arranged
Annotate: germination vigor calculates with the germination rate that germinateed the 6th day, all the other investigation results for germinateing the 21st day.
Claims (3)
1. Leymus chinensis seeds palea removing and temperature changing combination germinating method is characterized in that being made of following step:
1) seed cleaning impurity elimination
With machinery or the artificial selected impurity elimination of method, make seed purity and cleanliness should reach more than 90%;
2) go bran to handle
With medicament, manual or mechanical processing method are clever shell with the bran skin of Leymus chinensis seeds, partly or entirely remove or peel off;
3) seed disinfection
Take out the above-mentioned bran seeds treated of going, put into seed disinfectant and carried out surface disinfection 3-7 minute, thoroughly clean 3-4 time with clean water then and carry out seed sprouting again;
4) alternating temperature germinates
The Leymus chinensis seeds of sterilizing is seeded in the germinating bed, places low temperature 5-16 ℃, dark is under the situation of unglazed photograph, 12-16 hour; High temperature 28-35 ℃, under the light conditions, 12-8 hour; The time of low temperature, dark time and high temperature, illumination was to circulate in the cycle with 24 hours, under this alternating temperature condition germination 7-21 days.
2. Leymus chinensis seeds palea removing according to claim 1 and temperature changing combination germinating method is characterized in that the disinfectant that the seed disinfection process is used is 0.1% mercuric chloride solution, and the used germinating bed of alternating temperature germination process is soil, filter paper or sand germinating bed;
3. Leymus chinensis seeds palea removing according to claim 2 and temperature changing combination germinating method is characterized in that:
1) method is by hand carried out selected impurity elimination;
2) go bran to handle
Method is by hand all peeled the bran skin of Leymus chinensis seeds off;
3) seed disinfection
Behind the Leymus chinensis seeds surface disinfection 5 minutes, carry out seed sprouting again 4 times with thorough cleaning of clean water;
4) alternating temperature germinates
The Leymus chinensis seeds of sterilizing is seeded on the sand germinating bed, is positioned over 5 ℃ of low temperature, 5 ℃, 16 ℃, under the dark situation, 14 hours; 35 ℃, 28 ℃, 28 ℃ of high temperature, under the light conditions, 10 hours; Under this alternating temperature condition, germinateed 21 days; 35 ℃, 28 ℃, 28 ℃ of 5 ℃, 5 ℃, 16 ℃ corresponding high temperature of difference of low temperature that wherein above-mentioned alternating temperature germinates.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102293152A (en) * | 2011-07-08 | 2011-12-28 | 大连民族学院 | Combined germination method of hull peeling off and low temperature seed soaking for Bothriochloa ischaemum (L.) Keng |
| CN102948281A (en) * | 2012-11-13 | 2013-03-06 | 深圳职业技术学院 | Method for promoting germination of spider flower seeds |
| CN105053829A (en) * | 2015-09-09 | 2015-11-18 | 曾庆云 | Preparation method of winter wheat seed germ active peptide nutrient |
| CN115589941A (en) * | 2022-10-26 | 2023-01-13 | 中国科学院东北地理与农业生态研究所（Cn） | Method for quickly and efficiently obtaining saline-alkali-tolerant leymus chinensis mutant material |
-
2006
- 2006-12-14 CN CNA2006101632451A patent/CN101199251A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102293152A (en) * | 2011-07-08 | 2011-12-28 | 大连民族学院 | Combined germination method of hull peeling off and low temperature seed soaking for Bothriochloa ischaemum (L.) Keng |
| CN102948281A (en) * | 2012-11-13 | 2013-03-06 | 深圳职业技术学院 | Method for promoting germination of spider flower seeds |
| CN105053829A (en) * | 2015-09-09 | 2015-11-18 | 曾庆云 | Preparation method of winter wheat seed germ active peptide nutrient |
| CN115589941A (en) * | 2022-10-26 | 2023-01-13 | 中国科学院东北地理与农业生态研究所（Cn） | Method for quickly and efficiently obtaining saline-alkali-tolerant leymus chinensis mutant material |
| CN115589941B (en) * | 2022-10-26 | 2023-06-09 | 中国科学院东北地理与农业生态研究所 | Method for rapidly and efficiently obtaining saline-alkali tolerant leymus chinensis mutant material |
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Open date: 20080618 |