CN103797931A - Method for breaking dormancy of daphne giraldii seeds - Google Patents
Method for breaking dormancy of daphne giraldii seeds Download PDFInfo
- Publication number
- CN103797931A CN103797931A CN201410064619.9A CN201410064619A CN103797931A CN 103797931 A CN103797931 A CN 103797931A CN 201410064619 A CN201410064619 A CN 201410064619A CN 103797931 A CN103797931 A CN 103797931A
- Authority
- CN
- China
- Prior art keywords
- seed
- winter daphne
- seeds
- abolishing
- yellow winter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Pretreatment Of Seeds And Plants (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a method for breaking the dormancy of daphne giraldii seeds, belonging to the field of quality identification and cultivation of traditional Chinese medicine seeds. The method comprises the following steps: (1) disinfecting the seeds, namely soaking and disinfecting seeds to be tested by using a potassium hypermanganate solution; washing cleanly by distilled water; and (2) wetly preserving in a cellar, namely mixing the seeds of the step (1) and wet sand, and stacking into a vegetable cellar in piles for wet storage. The method for breaking the dormancy of the daphne giraldii seeds disclosed by the invention is simple, convenient and feasible and has a reliable principle, good repeatability and good effect; the dormancy of the daphne giraldii seeds can be rapidly broken and the germination rate and the germination potential of the daphne giraldii seeds can be effectively improved; the working efficiency is high and the repeatability is good; the germination rate of the daphne giraldii seeds is up to 30.47% and the germination potential is up to 18.12%.
Description
Technical field
The invention belongs to traditional Chinese medicine seed quality and identify and cultivation field, particularly one is abolished the seed dormant method of yellow winter daphne.
Technical background
Yellow winter daphne (Daphne giraldii Nitsche) belongs to Thymelaeceae daphne plant, another name knotting flower, Edgeworthia chrysantha.Be distributed in the provinces such as China Shaanxi, Gansu, Qinghai, Henan, Gansu Province is mainly distributed in hillside shrubbery, border, the cheuch area of the height above sea level 2000～2600m such as the Qilian mountains.Root skin and the stem skin of yellow winter daphne, for the conventional Chinese medicine for the treatment of rheumatism among the people and pain relieving, be commonly called as girald daphne bark, has the effects such as reducing blood lipid, anti-hemolysis activity, antitumor activity and strong analgesia, also can be used for treatment of arthritis, analgesia, anti-inflammatory, surgery anesthesia etc.The yellow winter daphne of plant is wild resource at present, is traditional Chinese medicine girald daphne bark's former plant, and using position is Gen Pi and stem skin, and the excessive unreasonable root that connects is excavated mode, makes plant resources be subject to heavy damage, causes the former plant of girald daphne bark extremely in short supply; Because yellow winter daphne seed has strong property and dormancy, germination rate is very low, is difficult to meet Production requirement again; This certainly will affect the breeding of yellow winter daphne plant and making full use of of yellow winter daphne plant medical value; In prior art, not about abolishing the report of the seed dormant method of yellow winter daphne, providing one to abolish the seed dormant method of yellow winter daphne is the task of top priority.
Summary of the invention
The object of this invention is to provide that a kind of simple and easy to do, principle is reliable, favorable repeatability, the effective seed dormant method of yellow winter daphne of abolishing, thisly abolish germination rate and the germination vigor that the seed dormant method of yellow winter daphne can be abolished fast the dormancy of yellow winter daphne seed, can effectively improve yellow winter daphne seed, operating efficiency is high, reproducible.
Content of the present invention and step are done to specific description below:
The present invention abolishes the seed dormant method of yellow winter daphne, and described method comprises the steps,
(1) seed disinfection: will be for planting experimentally son liquor potassic permanganate soaking disinfection, clean with distilled water flushing;
(2) wet Tibetan in cellar for storing things: be deposited in by heap the Tibetan of wetting in clamp after the seed that step (1) is obtained mixes with damp sand.
Inhibitor contents during interior wet Tibetan the in cellar for storing things in seed declines, softening, the saturating property of seed coat improves, embryo development maturation, for seed development provides strong condition, can in seed dormancy, obtain better effects breaking, the physical property dormancy that in cellar for storing things, wet Tibetan can be abolished seed efficiently, rapidly, improve the germination rate of seed, time saving and energy saving, easy to operate, efficiency is high.
The present invention abolishes the seed dormant method of yellow winter daphne, and in described step (1), the mass fraction of described liquor potassic permanganate is 0.2%.
The present invention abolishes the seed dormant method of yellow winter daphne, in described step (1), described for plant experimentally son liquor potassic permanganate soak time be 20min.
The present invention abolishes the seed dormant method of yellow winter daphne, and in described step (2), the humidity of described damp sand is 50%-70%, and preferably 60%.
The humidity of described damp sand be hold with damp sand can be agglomerating, pine can scatter and be as the criterion, in the time that the humidity of damp sand is 50%-70%, described damp sand is held can be agglomerating, pine can scatter, and in this humidity range, can impel yellow soft and soggyization of winter daphne seed, thoroughly property improve, embryo development, be conducive to that yellow winter daphne is seed dormant to be abolished; When the humidity of damp sand is 60%, abolish the most effectively the soonest to yellow winter daphne is seed dormant, can effectively improve germination rate and the germination vigor of yellow winter daphne seed.
The present invention abolishes the seed dormant method of yellow winter daphne, and in described step (2), the volume ratio of described seed and described wet sand is 1:3.
The present invention abolishes the seed dormant method of yellow winter daphne, and in described step (2), the stacking thickness that described seed is deposited in after mixing with damp sand in clamp is 30～50cm.
The present invention abolishes the seed dormant method of yellow winter daphne, and in described step (2), the stacking thickness that described seed is deposited in after mixing with damp sand in clamp is 40cm.
The present invention abolishes the seed dormant method of yellow winter daphne, and in described step (2), described seed and damp sand cover the damp sand that 6～16cm is thick after mixing and being incorporated in the interior stacking of clamp on stacked mulling seed.
The present invention abolishes the seed dormant method of yellow winter daphne, and in described step (2), in described cellar for storing things, the wet time of hiding is 20-100 days.
Wet Tibetan while processing in cellar for storing things, along with the lengthening of wet Tibetan time in cellar for storing things, the hard seed rate of seed reduces gradually, and the enzymic activity of Interior Seed improves, and has strengthened hydrolysis, promotes the inhibiting substances of Interior Seed to decompose, the dormancy of having abolished seed; And in the time that in cellar for storing things, the wet Tibetan time is oversize, seed again can be owing to going mouldy in moisture state for a long time, affects the germination of seed.
The present invention abolishes the seed dormant method of yellow winter daphne, and in described step (2), in described cellar for storing things, the wet time of hiding is 80 days.
When in cellar for storing things, the wet time of hiding is 80 days, the dormancy that both can effectively abolish seed, can prevent that again seed from going mouldy, and guarantees germination rate and the germination vigor of seed.
Germination vigor refers in the time that the Sino-Japan chitting piece number of germination process reaches top, the seed number of germinateing accounts for the percentage for test sample product seed number, generally take the germination test prescribed time-limit initial 1/3 during in seed sprouting number account for for the percentage of testing seed number as standard; Refer to that seed is in the number of days of germination test initial stage regulation (while counting for the first time), normal chitting piece number accounts for for the percentage of planting experimentally subnumber.It is the leading indicator of differentiating seed sprouting regularity.
The computing formula of germination vigor is as follows:
Germination vigor=(chitting piece number/test seed sum in alloted days) × 100%.(this test determination is 35d)
Germination rate refers to that seed is in the number of days of germination test initial stage regulation (while counting for the first time), and normal chitting piece number accounts for for the percentage of planting experimentally subnumber; Refer to that test seed sprouting number accounts for the percentage of test seed sum.Germination rate is the leading indicator of differentiating seed sprouting regularity, is one of important indicator detecting seed quality, usually calculates according to this sowing quantity in agricultural production.
The computing formula of germination rate is as follows:
Germination rate=(the total grain of total grain number/test number germinates) × 100%.
The beneficial effect of abolishing the seed dormant method of yellow winter daphne of the present invention is: the seed dormant method of yellow winter daphne of abolishing of the present invention, simple and easy to do, principle is reliable, favorable repeatability, effective, the dormancy that can abolish fast yellow winter daphne seed, germination rate and the germination vigor that can effectively improve yellow winter daphne seed, and operating efficiency is high, reproducible.
Embodiment
Below in conjunction with embodiment, the invention will be further described:
Embodiment 1
Abolish the seed dormant method of yellow winter daphne, comprise the steps:
(1) seed disinfection: by carrying out disinfection for planting experimentally the liquor potassic permanganate immersion 20min that son mass fraction is 0.2%, clean with distilled water flushing;
(2) wet Tibetan in cellar for storing things: the seed that step (1) is obtained and damp sand are deposited in wet Tibetan 80 days in clamp by heap after being mixed to form mulling seed with the volume ratio of 1:3, wherein, stacking thickness is 40cm, the humidity of described damp sand is 60%, will above the mulling seed of described stacking, cover the damp sand that 12cm is thick.
Measurement result: the germination rate of yellow winter daphne seed is 30.47%, germination vigor is 18.12%.
Embodiment 2
Abolish the seed dormant method of yellow winter daphne, comprise the steps:
(1) seed disinfection: by carrying out disinfection for planting experimentally the liquor potassic permanganate immersion 20min that son mass fraction is 0.2%, clean with distilled water flushing;
(2) wet Tibetan in cellar for storing things: the seed that step (1) is obtained and damp sand are deposited in wet Tibetan 20 days in clamp by heap after being mixed to form mulling seed with the volume ratio of 1:3, wherein, stacking thickness is 30cm, the humidity of described damp sand is 50%, will above the mulling seed of described stacking, cover the damp sand that 6cm is thick.
Measurement result: the germination rate of yellow winter daphne seed is 0%, germination vigor is 0%.
Embodiment 3
Abolish the seed dormant method of yellow winter daphne, comprise the steps:
(1) seed disinfection: by carrying out disinfection for planting experimentally the liquor potassic permanganate immersion 20min that son mass fraction is 0.2%, clean with distilled water flushing;
(2) wet Tibetan in cellar for storing things: the seed that step (1) is obtained and damp sand are deposited in wet Tibetan 100 days in clamp by heap after being mixed to form mulling seed with the volume ratio of 1:3, wherein, stacking thickness is 50cm, the humidity of described damp sand is 60%, will above the mulling seed of described stacking, cover the damp sand that 16cm is thick.
Measurement result: the germination rate of yellow winter daphne seed is 22.97%, germination vigor is 16.08%.
Embodiment 4
Abolish the seed dormant method of yellow winter daphne, comprise the steps:
(1) seed disinfection: by carrying out disinfection for planting experimentally the liquor potassic permanganate immersion 20min that son mass fraction is 0.2%, clean with distilled water flushing;
(2) wet Tibetan in cellar for storing things: the seed that step (1) is obtained and damp sand are deposited in wet Tibetan 50 days in clamp by heap after being mixed to form mulling seed with the volume ratio of 1:3, wherein, stacking thickness is 38cm, the humidity of described damp sand is 70%, will above the mulling seed of described stacking, cover the damp sand that 10cm is thick.
Measurement result: the germination rate of yellow winter daphne seed is 19.40%, germination vigor is 9.30%.
Claims (10)
1. abolish the seed dormant method of yellow winter daphne, it is characterized in that, described method comprises the steps,
(1) seed disinfection: will be for planting experimentally son liquor potassic permanganate soaking disinfection, clean with distilled water flushing;
(2) wet Tibetan in cellar for storing things: be deposited in by heap the Tibetan of wetting in clamp after the seed that step (1) is obtained mixes with damp sand.
2. the seed dormant method of yellow winter daphne of abolishing according to claim 1, is characterized in that, in described step (1), the mass fraction of described liquor potassic permanganate is 0.2%.
3. the seed dormant method of yellow winter daphne of abolishing according to claim 1, is characterized in that, in described step (1), described for plant experimentally son liquor potassic permanganate soak time be 20min.
4. the seed dormant method of yellow winter daphne of abolishing according to claim 1, is characterized in that, in described step (2), the humidity of described damp sand is 50%-70%, preferably 60%.
5. the seed dormant method of yellow winter daphne of abolishing according to claim 1, is characterized in that, in described step (2), the volume ratio of described seed and described wet sand is 1:3.
6. the seed dormant method of yellow winter daphne of abolishing according to claim 1, is characterized in that, in described step (2), the stacking thickness that described seed is deposited in after mixing with damp sand in clamp is 30～50cm.
7. the seed dormant method of yellow winter daphne of abolishing according to claim 6, is characterized in that, in described step (2), the stacking thickness that described seed is deposited in after mixing with damp sand in clamp is 40cm.
8. the seed dormant method of yellow winter daphne of abolishing according to claim 1, is characterized in that, in described step (2), described seed and damp sand cover the damp sand that 6～16cm is thick after mixing and being incorporated in the interior stacking of clamp on stacked mulling seed.
9. the seed dormant method of yellow winter daphne of abolishing according to claim 1, is characterized in that, in described cellar for storing things, the wet time of hiding is 20-100 days.
10. the seed dormant method of yellow winter daphne of abolishing according to claim 9, is characterized in that, in described cellar for storing things, the wet time of hiding is 80 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410064619.9A CN103797931B (en) | 2014-02-25 | 2014-02-25 | One abolishes the seed dormant method of Daphne Giraldii Nitsche |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410064619.9A CN103797931B (en) | 2014-02-25 | 2014-02-25 | One abolishes the seed dormant method of Daphne Giraldii Nitsche |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103797931A true CN103797931A (en) | 2014-05-21 |
| CN103797931B CN103797931B (en) | 2016-08-17 |
Family
ID=50695711
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410064619.9A Expired - Fee Related CN103797931B (en) | 2014-02-25 | 2014-02-25 | One abolishes the seed dormant method of Daphne Giraldii Nitsche |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103797931B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104081913A (en) * | 2014-06-10 | 2014-10-08 | 甘肃凯源生物技术开发中心 | Method for increasing germination rate of daphne giraldii nitsche seeds |
| CN107018704A (en) * | 2017-03-30 | 2017-08-08 | 河西学院 | A kind of method of raising Tang Gute winter daphne percentages of seedgermination |
| CN107046849A (en) * | 2017-03-28 | 2017-08-18 | 河西学院 | A kind of efficient germination accelerating method of D.giraldii Nitsche seed |
| CN108633375A (en) * | 2018-04-04 | 2018-10-12 | 赵崇来 | A method of abolishing wild D.giraldii Nitsche seed dormancy |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009232755A (en) * | 2008-03-27 | 2009-10-15 | National Agriculture & Food Research Organization | Treatment method for improving germination |
| CN102845199A (en) * | 2012-09-03 | 2013-01-02 | 甘肃泰康制药有限责任公司 | Artificial seed breeding technology of wild girald daphne bark |
-
2014
- 2014-02-25 CN CN201410064619.9A patent/CN103797931B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009232755A (en) * | 2008-03-27 | 2009-10-15 | National Agriculture & Food Research Organization | Treatment method for improving germination |
| CN102845199A (en) * | 2012-09-03 | 2013-01-02 | 甘肃泰康制药有限责任公司 | Artificial seed breeding technology of wild girald daphne bark |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104081913A (en) * | 2014-06-10 | 2014-10-08 | 甘肃凯源生物技术开发中心 | Method for increasing germination rate of daphne giraldii nitsche seeds |
| CN104081913B (en) * | 2014-06-10 | 2015-10-28 | 甘肃凯源生物技术开发中心 | A kind of method improving D.giraldii Nitsche percentage of seedgermination |
| CN107046849A (en) * | 2017-03-28 | 2017-08-18 | 河西学院 | A kind of efficient germination accelerating method of D.giraldii Nitsche seed |
| CN107018704A (en) * | 2017-03-30 | 2017-08-08 | 河西学院 | A kind of method of raising Tang Gute winter daphne percentages of seedgermination |
| CN108633375A (en) * | 2018-04-04 | 2018-10-12 | 赵崇来 | A method of abolishing wild D.giraldii Nitsche seed dormancy |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103797931B (en) | 2016-08-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Andrade et al. | Gas exchanges and growth of passion fruit under saline water irrigation and H 2 O 2 application | |
| CN101817708B (en) | Plant source seed soaking agent and preparation method thereof | |
| CN103797932B (en) | A kind of strong restructuring bankrupt promoting D.giraldii Nitsche seed sprouting | |
| CN104255281A (en) | Sealwort cultivation method | |
| CN103797931A (en) | Method for breaking dormancy of daphne giraldii seeds | |
| CN103718694B (en) | A kind of method promoting kwangsi mayten herb seed germination | |
| CN106258062B (en) | A kind of seed potato processing method | |
| CN105340530A (en) | Peper seed artificial breeding method | |
| CN104541659A (en) | Method for promoting germination of ardisia maclurei seeds | |
| CN106212172A (en) | Poem beautiful jade leads to cuttage and seedling culture method with a smile | |
| CN106489514A (en) | A kind of insect prevention implantation methods of booth vegetable | |
| CN104982449B (en) | Method for controlling weed growth and improving turf mature quality | |
| Amer et al. | Impact of foliar with nano-silica in mitigation of salt stress on some soil properties, crop-water productivity and anatomical structure of maize and faba bean | |
| Anburani et al. | Influence of seed treatment on the enhancement of germination and seedling vigour of papaya | |
| CN105850269B (en) | A kind of germinating method of the high lipid vegetable seeds of heavily fortified point shell | |
| CN100586268C (en) | Method for breeding saw palmetto | |
| CN102282930A (en) | Nitrogen applying method for quercus variabilis blume containerized seedlings | |
| CN104335886A (en) | Cultivation method of blueberry before transplantation | |
| CN104081913B (en) | A kind of method improving D.giraldii Nitsche percentage of seedgermination | |
| CN104012207A (en) | Method for improving seed germination capacity of water fennel | |
| CN103843638B (en) | A kind of method improving apocarya exposed root and shoot forestation survival rate | |
| Al-Menaie et al. | The effects of different treatments on seed germination of the Cassia fistula L. and Cassia nodosa Buch.-Ham. ex Roxb. in Kuwait | |
| CN105409563A (en) | Scion clipping processing method for improving cutting survival rate of ginkgo tree | |
| CN105850726B (en) | A kind of ginkgo breeding of new variety method of anti-stem rot | |
| CN113892425A (en) | Rapid cultivation method of hazelnut semi-aseptic seedlings |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160817 Termination date: 20190225 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |