CN102765988A - Lucid Ganoderma cultivation bag material and method for cultivating Lucid Ganoderma by same - Google Patents
Lucid Ganoderma cultivation bag material and method for cultivating Lucid Ganoderma by same Download PDFInfo
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- CN102765988A CN102765988A CN2012102479774A CN201210247977A CN102765988A CN 102765988 A CN102765988 A CN 102765988A CN 2012102479774 A CN2012102479774 A CN 2012102479774A CN 201210247977 A CN201210247977 A CN 201210247977A CN 102765988 A CN102765988 A CN 102765988A
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- 239000000463 material Substances 0.000 title claims abstract description 57
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Abstract
A Lucid Ganoderma cultivation bag material comprises, by weight percent, 25-35% of beanstalk crumbs, 20-30% of soybean dregs, 33-53% of sawdust, 2% of land plaster, and soybean whey which is 1-1.2 times of the cultivation bag material in weight. A method for cultivating Lucid Ganoderma by the Lucid Ganoderma cultivation bag material includes firstly, performing moist heat sterilization to the Lucid Ganoderma cultivation bag material at high temperature and high pressure, cooling, and inoculating a Lucid Ganoderma strain; secondly, cultivating with mycelia for 35-50 days to sprout; and thirdly, continuing to cultivate for 30 days, and sprouting and controlling to obtain ripe Lucid Ganoderma fruiting bodies. The soybean dregs contain sufficient phosphorus and vitamin, and the pH value, variation with time, of the soybean dregs fits with required environment for growth of Lucid Ganoderma. By the Lucid Ganoderma cultivation bag material, high-quality and high-yield Lucid Ganoderma fruiting bodies can grow, waste utilization of the beanstalk, the soybean dregs and the soybean whey is achieved, and primary chemical and medicinal compositions of the cultivated Lucid Ganoderma and content of Lucid Ganoderma triterpenes are higher than those of marketable Lucid Ganoderma.
Description
Technical field
The present invention relates to the method for a kind of cultivation of glossy ganoderma Bag Material and cultivation of glossy ganoderma thereof, relate in particular to a kind of method of utilizing beanstalk bits, bean dregs and yellow plasm of soybean as the cultivating in bag glossy ganoderma.
Background technology
Glossy ganoderma generic Aphyllophorales, Ganodermataceae is commonly called as Ganoderma lucidum seu Japonicum, auspicious grass, celestial grass, since ancient times just always as a kind of symbol of luckiness, the good medicine of curing the disease and natural artwork etc.Market potential is big, and development prospect is wide.In whole glossy ganoderma industry, the most important thing is cultivation of glossy ganoderma, the quality of glossy ganoderma directly influences the quality of its downstream converted products.
At present, with cultivation of glossy ganoderma and to use relevant one Chinese patent application existing 700 multinomial, with the technological related application of cultivation of glossy ganoderma then less than 10.Usually glossy ganoderma artificial culture material is main with wood chip, the linden of broad-leaf forest.Disclose a kind of culturing edible fungi like Chinese patent 200910041707.6, related in particular to a kind of ganoderma lucidum cultivation method that has used the cultivating materials of eucalyptus conduct.This invents described method for cultivating lucid ganoderma, more preferably contains bark or tree bar or the bark tree bar mixture of eucalyptus in its cultivating materials.This invention has selected for use those skilled in the art to think the eucalyptus that is inappropriate for cultivation of glossy ganoderma as cultivating materials in method for cultivating lucid ganoderma always, has obtained speed of growth Ganoderma sporophore faster, and wherein contained effective component of glossy ganoderma content is approaching in the sporophore.In addition, because eucalyptus grows soon, felling cycle is short, timber yield is high, so its price is lower than other broadleaf tree, and owing to need not add other compositions such as cotton seed hulls, bagasse, the ganoderma lucidum cultivation method that therefore should invent, cost is cheaper comparatively speaking.Yet, the waste material after the beanstalk stalk is gathered as soybean, lost mostly or on-site incineration as fertilizer, utilization ratio is low.Bean dregs are as the waste material of producing behind the bean product; Contain nutritive ingredients such as a large amount of food fibres, protein, fat; In addition as another waste material yellow plasm of soybean of producing bean product, wherein contain nutritive ingredient such as protein, fat, carbohydrate, soybean isoflavones, soybean saponin and cultivation of glossy ganoderma desired nutritional and fit like a glove.
As everyone knows, in the cultivation of glossy ganoderma technology, an amount of carbon source, nitrogenous source, mineral substance, VITAMINs need be provided, and the protein in beanstalk bits, bean dregs and the yellow plasm of soybean, sugar etc. can provide the sufficient nutrition material for the glossy ganoderma growth.The discovery that the contriver is surprised, it is high-quality to adopt sub product bean dregs, beanstalk bits and the yellow plasm of soybean of producing bean curd to grow, the Ganoderma sporophore of high yield.
Summary of the invention
One of the object of the invention provides a kind of cultivation of glossy ganoderma Bag Material.Two of the object of the invention provides a kind of method that adopts this cultivating in bag glossy ganoderma.Can not only grow Ganoderma sporophore high-quality, high yield, and solve the problem of the utilization of waste material of beanstalk, bean dregs, yellow seriflux.
The technical solution adopted for the present invention to solve the technical problems is:
A kind of cultivation of glossy ganoderma Bag Material contains wood chip, 2% terra alba and the yellow plasm of soybean of 1-1.2 times of cultivating materials quality of bean dregs, the 33-53% of beanstalk bits, the 20-30% of the cultivating materials component of following mass percent: 25-35%.Said bean dregs are for containing the bean dregs of protein 24.58 ± 2%, fat 10.5 ± 1%, moisture 10.52 ± 1%, ash content 4.3 ± 0.5%, total reducing sugar 5.09 ± 0.5%, robust fibre 45.01 ± 3%.Said yellow plasm of soybean (g/L) is for containing the yellow plasm of soybean of protein 4.08 ± 0.5, fat 1.10 ± 0.5, reducing sugar 0.53 ± 0.2, sucrose 1.83 ± 0.5, soybean isoflavones 0.62 ± 0.2, soybean saponin 0.42 ± 0.1.
Above-mentioned cultivation of glossy ganoderma Bag Material comprises following preparation process:
1) gets the raw materials ready: get the raw materials ready by above-mentioned component; The beanstalk stalk is shone dry grinding get the beanstalk bits; Dry bean dregs and wood chip;
2) be equipped with water: prepare cultivating materials quality 1-1.2 yellow plasm of soybean doubly;
3) batching: beanstalk bits add water and prewet that softening back adds wood chip and terra alba stirs, and add entry and bean dregs again, fully stir cultivating materials;
4) the cultivating materials plastics strain bag of packing into is processed the cultivation Bag Material.
Preferably, the particle size of beanstalk bits is 0.4-0.5cm in the described step 1).Wood chip is maple or Chinese chestnut tree wood chip.
Preferably, described step 3) softening time of prewetting is 24h.
Adopt the method for above-mentioned cultivation of glossy ganoderma cultivating in bag glossy ganoderma, comprise the steps:
5) the cultivating materials plastics strain bag of packing into is processed the cultivation Bag Material, through the HTHP moist heat sterilization, Ganderma lucidum strain is inserted in the cooling back;
6) went out sesame in mycelium culture 35-50 days;
7) continuation was cultivated 30 days, went out to obtain sophisticated Ganoderma sporophore after sesame is managed.
Preferably, the said culture bag of described step 5) adopts high-density low pressure polyethylene plastics, and specification is 16cm * 55cm or 20cm * 35cm back cover bag, and charging is wanted evenly, degree of tightness is appropriate; Sterilization is the normal-pressure boiler sterilization, and temperature rises to 100 ℃, keeps 20-25 hour; Then pressure rises to 0.15 MPa, keeps 2.5-3.0 hour; Temperature is reduced to about 60-70 ℃ in the bag heap, removes in the indoor or booth to deinsectization, the sterilization and discharges, and waits in the pocket temperature to reduce to and begins inoculation below 30 ℃.
Preferably, described step 6) cultivation is that the lucifuge blow-by is cultivated under 20-28 ℃ of temperature, 60-70% humidity.The control of first week of mycelium culture helps mycelia for 26-28 ℃ and restores sprouting material feeding base.Room temperature is reduced to about 22-25 ℃ and is cultivated mycelia afterwards.In the bacteria developing period later stage, cultivate indoor air temperature and be reduced to about 20 ℃.Mycelium culture began ventilation after 3 days, and ventilate once later every day, and each 0.5h is to 1h.Want turning once after 10 days, check and choose the cultivation material bag of pollution.
Preferably, the temperature that described step 7) goes out the sesame management is 22-28 ℃, and humidity is 85-95%.The planting type that goes out sesame management has tier rack type to put or accumbency is stacked puts or vertical type is put.The temperature of cultivating chamber should be controlled between 22 ~ 26 ℃ as far as possible.Temperature is crossed when hanging down, and available plastics film covers on the bacterium bag, and close the doors and windows evening, and hot water or air-conditioning system temperature are watered in ground.When temperature is too high, should be at the plug-in curtain that shelters from heat or light that adds of door and window, and at indoor suitable mist cooling.The carbon dioxide content that the suitable air of Ganoderma sporophore growth is requires 0.03 ~ 0.1%.The atmospheric moisture of Ganoderma sporophore growth should remain on 90 ~ 95%.When sporophore growth finalizes the design almost, but the cap edge should reduce AT to 85% when still being bright yellow.Along with sporophore vigorous growth, should give external moisturizing, utilize atomizer to spray in the interior space, the special-purpose humidifier of the available edible mushrooms of having ready conditions adds humidity.Light quantity requirement when when Ganoderma sporophore hacks the requirement of light quantity being higher than button.The light intensity that button needed when forming be 100 rein in about, promptly the people can look thing and the illuminance that can not see clearly.The illuminance that needs when sporophore is hacked be 200 rein in about, promptly be equivalent to the illumination under the fluorescent lamp.Light in the cultivating chamber is wanted evenly.
Positively effect of the present invention is: Ganoderma lucidum mycelium growth ph optimum 5-6, Ganoderma sporophore growth ph optimum 4-5.And have bean dregs and yellow plasm of soybean, its time dependent pH value and the glossy ganoderma required environmental facies of growing suitable among the present invention.Phosphorus is a kind of main composition composition of glossy ganoderma cell nuclear, and other mineral elements are to play a part to improve enzymic activity as the coenzyme material mostly, and requirement is very little, so a large amount of phosphorus of glossy ganoderma growth needs.The glossy ganoderma growth also needs a certain amount of vitamin substances.And contain 35.8 milligrams of ironys in each hectogram soya bean approximately, phosphorous 418 milligrams, also have vitamin A, B
1, B
2, D, E etc.Owing to contain competent phosphorus and VITAMINs in the bean dregs, and its time dependent pH value and the glossy ganoderma required environmental facies of growing suit.That embodiment proves that also the present invention can not only grow is high-quality, and the Ganoderma sporophore of high yield has solved the problem of the utilization of waste material of beanstalk, bean dregs, yellow plasm of soybean.And the main chemistry of the Ganoderma sporophore of the present invention cultivation and pharmaceutical compound Ganoderma triterpenoids content are apparently higher than commercially available glossy ganoderma.
In addition, adopt the present invention to prepare the cultivation of glossy ganoderma Bag Material and adopt the method for this cultivating in bag glossy ganoderma, sufficient raw not only, technology is simple and easy, cost is low, output is high, the spore powder bag is full, need not with grain, and instant effect is practiced thrift the weedtree material, is protected forest resources.The useless bacterium bag of planting glossy ganoderma can culturing edible fungus, do livestock and poultry, fish etc. cultures plastics, organic fertilizer, biogas fuel raw materials.
Embodiment
The preparation of embodiment one cultivation of glossy ganoderma Bag Material and adopt this cultivating in bag glossy ganoderma
Comprise the steps:
1) gets the raw materials ready: the beanstalk stalk is shone dry doubling pulverize to such an extent that the 0.4-0.5cm beanstalk is considered to be worth doing; Dry bean dregs and maple wood chip and Chinese chestnut tree wood chip mixing wood chip; Component ratio (mass percent) is: 35% beanstalk bits, 30% bean dregs, 33% wood chip, 2% terra alba;
2) be equipped with water: the yellow plasm of soybean of preparing 1.2 times of cultivating materials quality;
3) batching: beanstalk bits add the soybean yellow seriflux and prewet softeningly, add behind the 24h and mix wood chip and terra alba stirs, and add yellow plasm of soybean and bean dregs again, fully stir cultivating materials;
4) the cultivating materials 20cm * 35cm back cover bag of packing into is processed culture bag; Through the HTHP moist heat sterilization; In the booth after deinsectization, sterilization etc. in the pocket temperature reduce to and begin inoculation below 30 ℃, the Mount Taishan red ganoderma bacterial classification that bacterial classification selects for use Shandong hat creature sesame ltd to sell;
5) lucifuge blow-by cultivation went out sesame in 40 days under 20-28 ℃ of temperature, 60-70% humidity.26-28 ℃ of first week of mycelium culture control helps mycelia and restores sprouting material feeding base.Room temperature is reduced to about 22-25 ℃ and is cultivated mycelia afterwards.In the bacteria developing period later stage, cultivate indoor air temperature and be reduced to about 20 ℃.Mycelium culture began ventilation after 3 days, and ventilate once later every day, and each 0.5h is to 1h.Want turning once after 10 days, check and choose the cultivation material bag of pollution.
6) planting type that goes out the sesame management adopts tier rack type to put.The temperature of cultivating chamber is controlled between 22 ~ 26 ℃ as far as possible.Cross when hanging down, cover with plastics film on the bacterium bag, close the doors and windows evening, and hot water or air-conditioning system temperature are watered in ground.When too high, at the plug-in curtain that shelters from heat or light that adds of door and window, and at indoor suitable mist cooling.The control carbon dioxide content requires 0.03 ~ 0.1%, and the control air temperature should remain on 90 ~ 95%.
When sporophore growth finalizes the design almost, but the cap edge reduces AT to 85% when still being bright yellow.Along with sporophore vigorous growth, give external moisturizing, utilize atomizer to spray in the interior space.When Ganoderma sporophore hacks, the light quantity requirement the when requirement of light quantity is higher than button.The light intensity that button needed when forming be 100 rein in about.The illuminance that needs when sporophore is hacked be 200 rein in about.Make that the light in the cultivating chamber is even.Continuation was cultivated 30 days, went out to obtain sophisticated Ganoderma sporophore after sesame is managed.
The preparation of embodiment two cultivation of glossy ganoderma Bag Materials and adopt this cultivating in bag glossy ganoderma
Comprise the steps:
1) gets the raw materials ready: the beanstalk stalk is shone dry doubling pulverize to such an extent that the 0.4-0.5cm beanstalk is considered to be worth doing; Dry bean dregs and maple wood chip and Chinese chestnut tree wood chip mixing wood chip; Component ratio (mass percent) is: 25% beanstalk bits, 20% bean dregs, 53% wood chip, 2% terra alba;
2) be equipped with water: the yellow plasm of soybean of preparing 1.2 times of cultivating materials quality;
3) batching: beanstalk bits add the soybean yellow seriflux and prewet softeningly, add behind the 24h and mix wood chip and terra alba stirs, and add yellow plasm of soybean and bean dregs again, fully stir cultivating materials;
4) the cultivating materials 20cm * 35cm back cover bag of packing into is processed culture bag, through the HTHP moist heat sterilization, in the booth after deinsectization, sterilization etc. in the pocket temperature reduce to and begin inoculation below 30 ℃, Xinzhou Ganderma lucidum strain that bacterial classification selects for use edible mushrooms station, Fujian Province to sell;
The preparation of all the other cultivation of glossy ganoderma Bag Materials and adopt this cultivating in bag glossy ganoderma with embodiment one.
The preparation of embodiment three cultivation of glossy ganoderma Bag Materials and adopt this cultivating in bag glossy ganoderma
Comprise the steps:
1) gets the raw materials ready: the beanstalk stalk is shone dry doubling pulverize to such an extent that the 0.4-0.5cm beanstalk is considered to be worth doing; Dry bean dregs and maple wood chip and Chinese chestnut tree wood chip mixing wood chip; Component ratio (mass percent) is: 30% beanstalk bits, 25% bean dregs, 43% wood chip, 2% terra alba;
2) be equipped with water: the yellow plasm of soybean of preparing 1.2 times of cultivating materials quality;
3) batching: beanstalk bits add the soybean yellow seriflux and prewet softeningly, add behind the 24h and mix wood chip and terra alba stirs, and add yellow plasm of soybean and bean dregs again, fully stir cultivating materials;
4) the cultivating materials 20cm * 35cm back cover bag of packing into is processed culture bag; Through the HTHP moist heat sterilization; In the booth after deinsectization, sterilization etc. in the pocket temperature reduce to and begin inoculation below 30 ℃, bacterial classification is selected No. 1, glossy ganoderma Mount Huang that breeding strain center, Huangshan sells for use;
The preparation of all the other cultivation of glossy ganoderma Bag Materials and adopt this cultivating in bag glossy ganoderma with embodiment one.
The glossy ganoderma of embodiment four embodiment one, two, three and commercially available glossy ganoderma are extracted Ganoderma triterpenoids
Triterpene compound structure is complicated and unstable, is insoluble in water, is soluble in organic solvent, and generally organic solvents such as ethanol, chloroform or methyl alcohol commonly used extract under low temperature or normal temperature.Different solvents, extraction effect are also inequality.Ultraviolet spectrophotometry is widely used in triterpene Determination on content in the glossy ganoderma; With triterpene compound Oleanolic Acid, ursolic acid is reference substance, with perchloric acid and Glacial acetic acid min. 99.5-oxalic acid aldehyde colour developing, in certain concentration range; Its absorbancy and compounds content meet Beer's law, can carry out colorimetric assay.Yet, when this method of employing is directly measured the total triterpene contents in the glossy ganoderma, can occur than strong jamming, there is tangible plus deviation in its result.Should adopt tlc, this method is the ordinary method that the centering pharmaceutically active ingredient is analyzed, and as much as possible triterpene compound is separated with other components, gets rid of the interference of other components.Also available Ganodenic acid is standard substance, adopts the content of total triterpene in the colorimetric method for determining glossy ganoderma.The concrete experiment as follows:
1) glossy ganoderma among the embodiment one, two, three and commercially available Mount Taishan red ganoderma, Xinzhou glossy ganoderma, each three of No. 1 glossy ganodermas in Mount Huang.
2) with the glossy ganoderma drying and crushing, cross 40 mesh sieves, it is subsequent use to get Ganoderma powder;
3) accurately take by weighing Ganoderma powder 1g, be dissolved in the 40ml chloroform, ultrasonic extraction 30min filters, and filtrates with the chloroform constant volume in the 50ml volumetric flask;
4) the accurate chloroform solution 10ml that draws puts in the separating funnel, with saturated sodium hydrogencarbonate extraction 4 times, and each 10ml, combining extraction liquid is put in the 50ml volumetric flask;
5) be diluted to scale with saturated sodium hydrogen carbonate solution, derived sample solution;
6) this solution of absorption certain volume adds 0.4ml5% Vanillin-glacial acetic acid solution 1.00ml perchloric acid behind evaporate to dryness in 100 ℃ of water-baths, behind 60 ℃ of heating in water bath 15min, move in the ice-water bath, adding the 5.00ml glacial acetic acid, shakes up and is placed on room temperature;
7) behind the 15min with ultraviolet-visible beam split spectrometer working sample solution absorbency under 548 wavelength, calculate with calibration curve method.
Ganoderma total triterpenes content (%) detected result table
Annotate: sample the 1,2, the 3rd, embodiment one sample; Sample the 4,5, the 6th, embodiment two samples; Sample the 7,8, the 9th, embodiment three samples; 10, the 11, the 12nd, Mount Taishan red ganoderma, Xinzhou glossy ganoderma, No. 1 glossy ganoderma sample in Mount Huang.Test 1,2,3 is parallel test.Interpretation of result: adopt cultivation of glossy ganoderma Bag Material of the present invention and adopt the Ganoderma total triterpenes content of this cultivating in bag to be higher than commercially available glossy ganoderma.
Claims (8)
1. cultivation of glossy ganoderma Bag Material is characterized in that containing the cultivating materials component of following mass percent: the wood chip of the beanstalk bits of 25-35%, the bean dregs of 20-30%, 33-53%, 2% terra alba and cultivating materials quality 1-1.2 yellow plasm of soybean doubly; The beanstalk bar that dries that described beanstalk bits go mouldy for clean nothing is pulverized, and particle size is 0.4-0.5cm; Wood chip is maple wood chip or Chinese chestnut tree wood chip in described; Described bean dregs composition is: protein 24.58 ± 2%, fat 10.5 ± 1%, moisture 10.52 ± 1%, ash content 4.3 ± 0.5%, total reducing sugar 5.09 ± 0.5%, the bean dregs of robust fibre 45.01 ± 3%; Described yellow plasm of soybean contains composition (g/L): the yellow plasm of soybean of protein 4.08 ± 0.5, fat 1.10 ± 0.5, reducing sugar 0.53 ± 0.2, sucrose 1.83 ± 0.5, soybean isoflavones 0.62 ± 0.2, soybean saponin 0.42 ± 0.1.
2. like the preparation method of claim 1 cultivation of glossy ganoderma Bag Material, comprise following preparation process:
1) gets the raw materials ready: get the raw materials ready by component; The beanstalk stalk is shone dry doubling to be pulverized to such an extent that beanstalk is considered to be worth doing; Dry bean dregs and wood chip;
2) be equipped with water: prepare cultivating materials quality 1-1.2 yellow plasm of soybean doubly;
3) batching: beanstalk bits add water and prewet that softening back adds wood chip and terra alba stirs, and add yellow plasm of soybean and bean dregs again, fully stir cultivating materials;
4) the cultivating materials plastics strain bag of packing into is processed the cultivation Bag Material.
3. the preparation method of cultivation of glossy ganoderma Bag Material as claimed in claim 2 is characterized in that: the softening time of prewetting in the described step 3) is 24h.
4. the preparation method of cultivation of glossy ganoderma Bag Material as claimed in claim 2 is characterized in that: the plastics strain bag is high-density low pressure polyethylene plastics in the described step 4), and specification is wide * and length is: 16cm * 55cm or 20cm * 35cm back cover bag.
5. adopt the method for the described cultivation of glossy ganoderma cultivating in bag of claim 1 glossy ganoderma, comprise the steps:
1) with the cultivation of glossy ganoderma Bag Material, through the HTHP moist heat sterilization, Ganderma lucidum strain is inserted in the cooling back;
2) went out sesame in mycelium culture 35-50 days;
3) continuation was cultivated 30 days, went out to obtain sophisticated Ganoderma sporophore after sesame is managed.
6. the method for cultivating ganoderma as claimed in claim 5 is characterized in that: the culture bag sterilization is the normal-pressure boiler sterilization in the described step 1), and temperature rises to 100 ℃, and maintenance is more than 20 hours; Then pressure rises to 0.15 MPa, keeps 2.5-3.0 hour; Temperature is reduced to about 60-70 ℃ in the said culture bag heap, raises coverture and is carried in the indoor or booth after deinsectization, the sterilization in the pocket such as discharging temperature while hot and reduces to and begin below 30 ℃ to inoculate.
7. the method for cultivating ganoderma as claimed in claim 5 is characterized in that: described step 2), and 26-28 ℃ of first week control; Room temperature is reduced to about 22-25 ℃ afterwards; In the bacteria developing period later stage, cultivate indoor air temperature and be reduced to about 20 ℃; Mycelium culture after 3 days every day ventilate once, each 0.5h is to 1h.
8. the method for cultivating ganoderma as claimed in claim 5 is characterized in that: described step 3) goes out sesame management temperature and is controlled between 22 ~ 28 ℃; AT should remain on 90 ~ 95%.
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| CN103704018A (en) * | 2013-12-20 | 2014-04-09 | 河北大学 | Method for preparing pleurotus salmoneostramineus liquid spawns with yellow serofluid as principal raw materials |
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| CN104082033A (en) * | 2014-06-10 | 2014-10-08 | 信芝（厦门）生物科技有限公司 | Ganoderma lucidum planting method |
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| CN103936512B (en) * | 2014-04-21 | 2016-02-03 | 山东省农业科学院农业资源与环境研究所 | Ganodermataceae Dook culture material and making method |
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| CN106852496A (en) * | 2015-12-09 | 2017-06-16 | 黄建钧 | The method of the triterpenes synthesis contained by induction mushroom gill fungus |
| CN106472106A (en) * | 2016-09-30 | 2017-03-08 | 桂林淮安天然保健品开发有限公司 | A kind of ganoderma lucidum cultivation method improving ganoderma polyoses content |
| CN107814597A (en) * | 2017-11-13 | 2018-03-20 | 湖北工业大学 | The preparation method of purple sesame culture medium |
| CN108061720A (en) * | 2017-12-26 | 2018-05-22 | 广州广电计量检测股份有限公司 | A kind of method that UV-absorptivity method measures ganodenic acid content |
| CN108887089A (en) * | 2018-10-09 | 2018-11-27 | 湖北悟芝堂生物科技有限公司 | A kind of dedicated culture material of cultivating in bag ganoderma lucidum and preparation method thereof |
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