CN102421791A - 稳定化逆转录酶融合蛋白 - Google Patents

稳定化逆转录酶融合蛋白 Download PDF

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Publication number
CN102421791A
CN102421791A CN2010800201231A CN201080020123A CN102421791A CN 102421791 A CN102421791 A CN 102421791A CN 2010800201231 A CN2010800201231 A CN 2010800201231A CN 201080020123 A CN201080020123 A CN 201080020123A CN 102421791 A CN102421791 A CN 102421791A
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China
Prior art keywords
reverse transcriptase
seq
fusion protein
protein
stabilization
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Pending
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CN2010800201231A
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English (en)
Chinese (zh)
Inventor
A·M·拉姆博维茨
S·摩尔
G·摩尔
E·加尼姆
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University of Texas System
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University of Texas System
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Publication of CN102421791A publication Critical patent/CN102421791A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/24—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a MBP (maltose binding protein)-tag

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN2010800201231A 2009-03-04 2010-03-04 稳定化逆转录酶融合蛋白 Pending CN102421791A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US15733209P 2009-03-04 2009-03-04
US61/157332 2009-03-04
PCT/US2010/026165 WO2010102085A2 (en) 2009-03-04 2010-03-04 Stabilized reverse transcriptase fusion proteins

Publications (1)

Publication Number Publication Date
CN102421791A true CN102421791A (zh) 2012-04-18

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CN2010800201231A Pending CN102421791A (zh) 2009-03-04 2010-03-04 稳定化逆转录酶融合蛋白

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US (4) US10113156B2 (enExample)
EP (2) EP2403941B1 (enExample)
JP (2) JP5882064B2 (enExample)
CN (1) CN102421791A (enExample)
AU (1) AU2010221284B2 (enExample)
BR (1) BRPI1009221A2 (enExample)
CA (1) CA2754476A1 (enExample)
IL (1) IL214913A (enExample)
MX (1) MX2011009205A (enExample)
WO (1) WO2010102085A2 (enExample)
ZA (1) ZA201107207B (enExample)

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US9012183B2 (en) 2011-02-23 2015-04-21 Board Of Regents, The University Of Texas System Use of template switching for DNA synthesis
US9340799B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College MRNA-sensing switchable gRNAs
US12043852B2 (en) 2015-10-23 2024-07-23 President And Fellows Of Harvard College Evolved Cas9 proteins for gene editing
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KR20250103795A (ko) 2016-08-03 2025-07-07 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 아데노신 핵염기 편집제 및 그의 용도
CN109804066A (zh) 2016-08-09 2019-05-24 哈佛大学的校长及成员们 可编程cas9-重组酶融合蛋白及其用途
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
AU2017342543B2 (en) 2016-10-14 2024-06-27 President And Fellows Of Harvard College AAV delivery of nucleobase editors
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
EP3592381A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Cancer vaccine
EP3592853A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression of pain by gene editing
KR20190127797A (ko) 2017-03-10 2019-11-13 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 시토신에서 구아닌으로의 염기 편집제
CA3057192A1 (en) 2017-03-23 2018-09-27 President And Fellows Of Harvard College Nucleobase editors comprising nucleic acid programmable dna binding proteins
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
CN111801345A (zh) 2017-07-28 2020-10-20 哈佛大学的校长及成员们 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物
WO2019139645A2 (en) 2017-08-30 2019-07-18 President And Fellows Of Harvard College High efficiency base editors comprising gam
US11352611B2 (en) 2017-10-03 2022-06-07 Board Of Regents, The University Of Texas System Non-LTR-retroelement reverse transcriptase and uses thereof
CA3082251A1 (en) 2017-10-16 2019-04-25 The Broad Institute, Inc. Uses of adenosine base editors
EP3724214A4 (en) 2017-12-15 2021-09-01 The Broad Institute Inc. SYSTEMS AND METHODS FOR PREDICTING REPAIR RESULTS IN GENETIC ENGINEERING
WO2019226953A1 (en) 2018-05-23 2019-11-28 The Broad Institute, Inc. Base editors and uses thereof
US12281338B2 (en) 2018-10-29 2025-04-22 The Broad Institute, Inc. Nucleobase editors comprising GeoCas9 and uses thereof
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
DE112020001306T5 (de) 2019-03-19 2022-01-27 Massachusetts Institute Of Technology Verfahren und zusammensetzungen zur editierung von nukleotidsequenzen
US12473543B2 (en) 2019-04-17 2025-11-18 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
CN114258398A (zh) 2019-06-13 2022-03-29 总医院公司 工程化的人内源性病毒样颗粒及使用其递送至细胞的方法
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
WO2021165273A1 (en) * 2020-02-17 2021-08-26 Leadxpro Ag Pro-macrobodies for the enhancement of structure research
AU2021230546A1 (en) 2020-03-04 2022-10-13 Flagship Pioneering Innovations Vi, Llc Methods and compositions for modulating a genome
JP2023525304A (ja) 2020-05-08 2023-06-15 ザ ブロード インスティテュート，インコーポレーテッド 標的二本鎖ヌクレオチド配列の両鎖同時編集のための方法および組成物
BR112023001272A2 (pt) 2020-07-24 2023-04-04 Massachusetts Gen Hospital Partículas semelhantes a vírus aprimoradas e métodos de uso das mesmas para entrega às células
CA3193099A1 (en) 2020-09-24 2022-03-31 David R. Liu Prime editing guide rnas, compositions thereof, and methods of using the same
CA3203876A1 (en) 2021-01-11 2022-07-14 David R. Liu Prime editor variants, constructs, and methods for enhancing prime editing efficiency and precision
WO2023015309A2 (en) 2021-08-06 2023-02-09 The Broad Institute, Inc. Improved prime editors and methods of use
CA3231677A1 (en) 2021-09-08 2023-03-16 Flagship Pioneering Innovations Vi, Llc Methods and compositions for modulating a genome
US20240417719A1 (en) 2021-10-25 2024-12-19 The Broad Institute, Inc. Methods and compositions for editing a genome with prime editing and a recombinase
IL313146A (en) 2021-12-03 2024-07-01 Harvard College Self-assembling virus-like particles for delivery of prime editors and methods of making and using same
WO2023205687A1 (en) 2022-04-20 2023-10-26 The Broad Institute, Inc. Improved prime editing methods and compositions
WO2023240137A1 (en) 2022-06-08 2023-12-14 The Board Institute, Inc. Evolved cas14a1 variants, compositions, and methods of making and using same in genome editing
WO2024077267A1 (en) 2022-10-07 2024-04-11 The Broad Institute, Inc. Prime editing methods and compositions for treating triplet repeat disorders
EP4619515A1 (en) 2022-11-17 2025-09-24 The Broad Institute, Inc. Prime editor delivery by aav
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WO2024206125A1 (en) 2023-03-24 2024-10-03 The Broad Institute, Inc. Use of prime editing for treating sickle cell disease
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US20080118949A1 (en) * 2004-08-13 2008-05-22 New England Biolabs, Inc. Intracellular Production Of A Nuclease

Also Published As

Publication number Publication date
US10150955B2 (en) 2018-12-11
JP2012519489A (ja) 2012-08-30
JP2015193666A (ja) 2015-11-05
AU2010221284B2 (en) 2015-10-01
EP2403941A2 (en) 2012-01-11
US20180340158A1 (en) 2018-11-29
EP2403941B1 (en) 2018-10-03
WO2010102085A4 (en) 2011-01-06
IL214913A0 (en) 2011-11-30
MX2011009205A (es) 2011-09-30
AU2010221284A1 (en) 2011-10-27
ZA201107207B (en) 2012-12-27
WO2010102085A3 (en) 2010-11-18
WO2010102085A2 (en) 2010-09-10
US10113156B2 (en) 2018-10-30
US20180346890A1 (en) 2018-12-06
JP5882064B2 (ja) 2016-03-09
US10858636B2 (en) 2020-12-08
EP3415621A1 (en) 2018-12-19
IL214913A (en) 2017-06-29
EP2403941A4 (en) 2012-09-05
US20120009630A1 (en) 2012-01-12
US20160289652A1 (en) 2016-10-06
CA2754476A1 (en) 2010-09-10
BRPI1009221A2 (pt) 2016-03-15

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