CN102421791A - 稳定化逆转录酶融合蛋白 - Google Patents

稳定化逆转录酶融合蛋白 Download PDF

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Publication number
CN102421791A
CN102421791A CN2010800201231A CN201080020123A CN102421791A CN 102421791 A CN102421791 A CN 102421791A CN 2010800201231 A CN2010800201231 A CN 2010800201231A CN 201080020123 A CN201080020123 A CN 201080020123A CN 102421791 A CN102421791 A CN 102421791A
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China
Prior art keywords
reverse transcriptase
seq
fusion protein
protein
stabilization
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Pending
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CN2010800201231A
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English (en)
Chinese (zh)
Inventor
A·M·拉姆博维茨
S·摩尔
G·摩尔
E·加尼姆
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The University of Texas System
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The University of Texas System
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Publication of CN102421791A publication Critical patent/CN102421791A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/24—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a MBP (maltose binding protein)-tag

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN2010800201231A 2009-03-04 2010-03-04 稳定化逆转录酶融合蛋白 Pending CN102421791A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US15733209P 2009-03-04 2009-03-04
US61/157332 2009-03-04
PCT/US2010/026165 WO2010102085A2 (en) 2009-03-04 2010-03-04 Stabilized reverse transcriptase fusion proteins

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CN102421791A true CN102421791A (zh) 2012-04-18

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CN2010800201231A Pending CN102421791A (zh) 2009-03-04 2010-03-04 稳定化逆转录酶融合蛋白

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US (4) US10113156B2 (enExample)
EP (2) EP3415621A1 (enExample)
JP (2) JP5882064B2 (enExample)
CN (1) CN102421791A (enExample)
AU (1) AU2010221284B2 (enExample)
BR (1) BRPI1009221A2 (enExample)
CA (1) CA2754476A1 (enExample)
IL (1) IL214913A (enExample)
MX (1) MX2011009205A (enExample)
WO (1) WO2010102085A2 (enExample)
ZA (1) ZA201107207B (enExample)

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CN116694740A (zh) * 2018-08-08 2023-09-05 加利福尼亚大学董事会 用于跨非连续模板的有序和连续的互补DNA（cDNA）合成的组合物和方法
CN116814581A (zh) * 2022-10-13 2023-09-29 苏州拜科生物科技有限公司 一种热稳定性反转录酶突变体基因、制备方法和应用

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KR101911969B1 (ko) * 2011-02-23 2018-10-25 보드 오브 리전츠, 더 유니버시티 오브 텍사스 시스템 Ｄｎａ 합성을 위한 주형 스위칭의 용도
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US9322037B2 (en) 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
WO2017070632A2 (en) 2015-10-23 2017-04-27 President And Fellows Of Harvard College Nucleobase editors and uses thereof
WO2017100177A1 (en) 2015-12-07 2017-06-15 Bio-Rad Laboratories, Inc. Dimeric reverse transcriptase
WO2018027078A1 (en) 2016-08-03 2018-02-08 President And Fellows Of Harard College Adenosine nucleobase editors and uses thereof
AU2017308889B2 (en) 2016-08-09 2023-11-09 President And Fellows Of Harvard College Programmable Cas9-recombinase fusion proteins and uses thereof
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
KR20240007715A (ko) 2016-10-14 2024-01-16 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵염기 에디터의 aav 전달
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
KR20190123328A (ko) 2017-03-09 2019-10-31 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 암 백신
WO2018165504A1 (en) 2017-03-09 2018-09-13 President And Fellows Of Harvard College Suppression of pain by gene editing
CN110914310A (zh) 2017-03-10 2020-03-24 哈佛大学的校长及成员们 胞嘧啶至鸟嘌呤碱基编辑器
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US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
EP3658573A1 (en) 2017-07-28 2020-06-03 President and Fellows of Harvard College Methods and compositions for evolving base editors using phage-assisted continuous evolution (pace)
EP3676376B1 (en) 2017-08-30 2025-01-15 President and Fellows of Harvard College High efficiency base editors comprising gam
WO2019070843A1 (en) * 2017-10-03 2019-04-11 Board Of Regents, The University Of Texas System INVERSE TRANSCRIPTASE WITH LTR-FREE BACKLIGHTS AND USES THEREOF
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US12406749B2 (en) 2017-12-15 2025-09-02 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
WO2019226953A1 (en) 2018-05-23 2019-11-28 The Broad Institute, Inc. Base editors and uses thereof
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WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
WO2020191241A1 (en) 2019-03-19 2020-09-24 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
WO2020214842A1 (en) 2019-04-17 2020-10-22 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
US20220259617A1 (en) 2019-06-13 2022-08-18 The General Hospital Corporation Engineered human-endogenous virus-like particles and methods of use thereof for delivery to cells
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JP7839094B2 (ja) * 2020-02-17 2026-04-01 リーデクスプロ アーゲー 構造研究の促進のためのｐｒｏ－マクロボディ
BR112022017715A2 (pt) 2020-03-04 2022-11-16 Flagship Pioneering Innovations Vi Llc Métodos e composições para modular um genoma
CA3177481A1 (en) 2020-05-08 2021-11-11 David R. Liu Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence
BR112023001272A2 (pt) 2020-07-24 2023-04-04 Massachusetts Gen Hospital Partículas semelhantes a vírus aprimoradas e métodos de uso das mesmas para entrega às células
CA3193099A1 (en) 2020-09-24 2022-03-31 David R. Liu Prime editing guide rnas, compositions thereof, and methods of using the same
AU2022206476A1 (en) 2021-01-11 2023-07-20 President And Fellows Of Harvard College Prime editor variants, constructs, and methods for enhancing prime editing efficiency and precision
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US20230141630A1 (en) * 2021-08-17 2023-05-11 New England Biolabs, Inc. Rolling Circle Reverse Transcription of Circular RNA
KR20260037664A (ko) 2021-09-08 2026-03-18 플래그쉽 파이어니어링 이노베이션스 브이아이, 엘엘씨 게놈을 조절하기 위한 방법 및 조성물
US20240417719A1 (en) 2021-10-25 2024-12-19 The Broad Institute, Inc. Methods and compositions for editing a genome with prime editing and a recombinase
IL313146A (en) 2021-12-03 2024-07-01 Harvard College Self-assembling virus-like particles for delivery of prime editors and methods of making and using same
WO2023205687A1 (en) 2022-04-20 2023-10-26 The Broad Institute, Inc. Improved prime editing methods and compositions
WO2023240137A1 (en) 2022-06-08 2023-12-14 The Board Institute, Inc. Evolved cas14a1 variants, compositions, and methods of making and using same in genome editing
WO2024077267A1 (en) 2022-10-07 2024-04-11 The Broad Institute, Inc. Prime editing methods and compositions for treating triplet repeat disorders
WO2024108092A1 (en) 2022-11-17 2024-05-23 The Broad Institute, Inc. Prime editor delivery by aav
EP4662306A2 (en) 2023-02-09 2025-12-17 The Broad Institute Inc. Evolved recombinases for editing a genome in combination with prime editing
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CN121086027B (zh) * 2025-09-03 2026-07-28 湖南艾科瑞生物工程有限公司 一种mmlv逆转录酶的多肽配体及其应用

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CN116694740A (zh) * 2018-08-08 2023-09-05 加利福尼亚大学董事会 用于跨非连续模板的有序和连续的互补DNA（cDNA）合成的组合物和方法
CN116814581A (zh) * 2022-10-13 2023-09-29 苏州拜科生物科技有限公司 一种热稳定性反转录酶突变体基因、制备方法和应用

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AU2010221284B2 (en) 2015-10-01
IL214913A (en) 2017-06-29
WO2010102085A4 (en) 2011-01-06
US20120009630A1 (en) 2012-01-12
WO2010102085A2 (en) 2010-09-10
JP5882064B2 (ja) 2016-03-09
US20180346890A1 (en) 2018-12-06
US20160289652A1 (en) 2016-10-06
EP2403941B1 (en) 2018-10-03
US10113156B2 (en) 2018-10-30
CA2754476A1 (en) 2010-09-10
MX2011009205A (es) 2011-09-30
US20180340158A1 (en) 2018-11-29
EP3415621A1 (en) 2018-12-19
US10150955B2 (en) 2018-12-11
ZA201107207B (en) 2012-12-27
BRPI1009221A2 (pt) 2016-03-15
WO2010102085A3 (en) 2010-11-18
JP2015193666A (ja) 2015-11-05
EP2403941A2 (en) 2012-01-11
US10858636B2 (en) 2020-12-08
AU2010221284A1 (en) 2011-10-27
IL214913A0 (en) 2011-11-30
EP2403941A4 (en) 2012-09-05
JP2012519489A (ja) 2012-08-30

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