JP5882064B2 - 安定化された逆転写酵素融合タンパク質 - Google Patents

安定化された逆転写酵素融合タンパク質 Download PDF

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Publication number
JP5882064B2
JP5882064B2 JP2011553101A JP2011553101A JP5882064B2 JP 5882064 B2 JP5882064 B2 JP 5882064B2 JP 2011553101 A JP2011553101 A JP 2011553101A JP 2011553101 A JP2011553101 A JP 2011553101A JP 5882064 B2 JP5882064 B2 JP 5882064B2
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seq
reverse transcriptase
protein
fusion protein
stabilized
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JP2011553101A
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Japanese (ja)
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JP2012519489A5 (enExample
JP2012519489A (ja
Inventor
アラン エム． ランボウィッツ，
アラン エム． ランボウィッツ，
サビーン モア，
サビーン モア，
ジョージ モア，
ジョージ モア，
エマン グハネム，
エマン グハネム，
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The University of Texas System
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The University of Texas System
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/24—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a MBP (maltose binding protein)-tag

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2011553101A 2009-03-04 2010-03-04 安定化された逆転写酵素融合タンパク質 Active JP5882064B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US15733209P 2009-03-04 2009-03-04
US61/157,332 2009-03-04
PCT/US2010/026165 WO2010102085A2 (en) 2009-03-04 2010-03-04 Stabilized reverse transcriptase fusion proteins

Related Child Applications (1)

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JP2015153175A Division JP2015193666A (ja) 2009-03-04 2015-08-03 安定化された逆転写酵素融合タンパク質

Publications (3)

Publication Number Publication Date
JP2012519489A JP2012519489A (ja) 2012-08-30
JP2012519489A5 JP2012519489A5 (enExample) 2013-03-14
JP5882064B2 true JP5882064B2 (ja) 2016-03-09

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JP2011553101A Active JP5882064B2 (ja) 2009-03-04 2010-03-04 安定化された逆転写酵素融合タンパク質
JP2015153175A Withdrawn JP2015193666A (ja) 2009-03-04 2015-08-03 安定化された逆転写酵素融合タンパク質

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JP2015153175A Withdrawn JP2015193666A (ja) 2009-03-04 2015-08-03 安定化された逆転写酵素融合タンパク質

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US (4) US10113156B2 (enExample)
EP (2) EP3415621A1 (enExample)
JP (2) JP5882064B2 (enExample)
CN (1) CN102421791A (enExample)
AU (1) AU2010221284B2 (enExample)
BR (1) BRPI1009221A2 (enExample)
CA (1) CA2754476A1 (enExample)
IL (1) IL214913A (enExample)
MX (1) MX2011009205A (enExample)
WO (1) WO2010102085A2 (enExample)
ZA (1) ZA201107207B (enExample)

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WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
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JP2021533751A (ja) * 2018-08-08 2021-12-09 ザ リージェンツ オブ ザ ユニヴァーシティ オブ カリフォルニアThe Regents of the University of California 不連続な複数の鋳型から相補的ＤＮＡ（ｃＤＮＡ）を順序だてて連続的に合成するための組成物およびその方法
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Publication number Publication date
AU2010221284B2 (en) 2015-10-01
IL214913A (en) 2017-06-29
WO2010102085A4 (en) 2011-01-06
CN102421791A (zh) 2012-04-18
US20120009630A1 (en) 2012-01-12
WO2010102085A2 (en) 2010-09-10
US20180346890A1 (en) 2018-12-06
US20160289652A1 (en) 2016-10-06
EP2403941B1 (en) 2018-10-03
US10113156B2 (en) 2018-10-30
CA2754476A1 (en) 2010-09-10
MX2011009205A (es) 2011-09-30
US20180340158A1 (en) 2018-11-29
EP3415621A1 (en) 2018-12-19
US10150955B2 (en) 2018-12-11
ZA201107207B (en) 2012-12-27
BRPI1009221A2 (pt) 2016-03-15
WO2010102085A3 (en) 2010-11-18
JP2015193666A (ja) 2015-11-05
EP2403941A2 (en) 2012-01-11
US10858636B2 (en) 2020-12-08
AU2010221284A1 (en) 2011-10-27
IL214913A0 (en) 2011-11-30
EP2403941A4 (en) 2012-09-05
JP2012519489A (ja) 2012-08-30

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