JP5882064B2 - 安定化された逆転写酵素融合タンパク質 - Google Patents

安定化された逆転写酵素融合タンパク質 Download PDF

Info

Publication number
JP5882064B2
JP5882064B2 JP2011553101A JP2011553101A JP5882064B2 JP 5882064 B2 JP5882064 B2 JP 5882064B2 JP 2011553101 A JP2011553101 A JP 2011553101A JP 2011553101 A JP2011553101 A JP 2011553101A JP 5882064 B2 JP5882064 B2 JP 5882064B2
Authority
JP
Japan
Prior art keywords
seq
reverse transcriptase
protein
fusion protein
stabilized
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2011553101A
Other languages
English (en)
Japanese (ja)
Other versions
JP2012519489A (ja
JP2012519489A5 (enExample
Inventor
アラン エム． ランボウィッツ，
アラン エム． ランボウィッツ，
サビーン モア，
サビーン モア，
ジョージ モア，
ジョージ モア，
エマン グハネム，
エマン グハネム，
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Texas System
Original Assignee
University of Texas System
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Texas System filed Critical University of Texas System
Publication of JP2012519489A publication Critical patent/JP2012519489A/ja
Publication of JP2012519489A5 publication Critical patent/JP2012519489A5/ja
Application granted granted Critical
Publication of JP5882064B2 publication Critical patent/JP5882064B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/24—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a MBP (maltose binding protein)-tag

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2011553101A 2009-03-04 2010-03-04 安定化された逆転写酵素融合タンパク質 Active JP5882064B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US15733209P 2009-03-04 2009-03-04
US61/157,332 2009-03-04
PCT/US2010/026165 WO2010102085A2 (en) 2009-03-04 2010-03-04 Stabilized reverse transcriptase fusion proteins

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2015153175A Division JP2015193666A (ja) 2009-03-04 2015-08-03 安定化された逆転写酵素融合タンパク質

Publications (3)

Publication Number Publication Date
JP2012519489A JP2012519489A (ja) 2012-08-30
JP2012519489A5 JP2012519489A5 (enExample) 2013-03-14
JP5882064B2 true JP5882064B2 (ja) 2016-03-09

Family

ID=42710216

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2011553101A Active JP5882064B2 (ja) 2009-03-04 2010-03-04 安定化された逆転写酵素融合タンパク質
JP2015153175A Withdrawn JP2015193666A (ja) 2009-03-04 2015-08-03 安定化された逆転写酵素融合タンパク質

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2015153175A Withdrawn JP2015193666A (ja) 2009-03-04 2015-08-03 安定化された逆転写酵素融合タンパク質

Country Status (11)

Country Link
US (4) US10113156B2 (enExample)
EP (2) EP2403941B1 (enExample)
JP (2) JP5882064B2 (enExample)
CN (1) CN102421791A (enExample)
AU (1) AU2010221284B2 (enExample)
BR (1) BRPI1009221A2 (enExample)
CA (1) CA2754476A1 (enExample)
IL (1) IL214913A (enExample)
MX (1) MX2011009205A (enExample)
WO (1) WO2010102085A2 (enExample)
ZA (1) ZA201107207B (enExample)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101818126B1 (ko) * 2011-02-09 2018-01-15 (주)바이오니아 열안정성이 증가된 역전사효소
US9012183B2 (en) 2011-02-23 2015-04-21 Board Of Regents, The University Of Texas System Use of template switching for DNA synthesis
US9340799B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College MRNA-sensing switchable gRNAs
US12043852B2 (en) 2015-10-23 2024-07-23 President And Fellows Of Harvard College Evolved Cas9 proteins for gene editing
EP3387002A4 (en) 2015-12-07 2019-04-24 Bio-Rad Laboratories, Inc. DIM REVERSE TRANSCRIPTASE
KR20250103795A (ko) 2016-08-03 2025-07-07 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 아데노신 핵염기 편집제 및 그의 용도
CN109804066A (zh) 2016-08-09 2019-05-24 哈佛大学的校长及成员们 可编程cas9-重组酶融合蛋白及其用途
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
AU2017342543B2 (en) 2016-10-14 2024-06-27 President And Fellows Of Harvard College AAV delivery of nucleobase editors
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
EP3592381A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Cancer vaccine
EP3592853A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression of pain by gene editing
KR20190127797A (ko) 2017-03-10 2019-11-13 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 시토신에서 구아닌으로의 염기 편집제
CA3057192A1 (en) 2017-03-23 2018-09-27 President And Fellows Of Harvard College Nucleobase editors comprising nucleic acid programmable dna binding proteins
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
CN111801345A (zh) 2017-07-28 2020-10-20 哈佛大学的校长及成员们 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物
WO2019139645A2 (en) 2017-08-30 2019-07-18 President And Fellows Of Harvard College High efficiency base editors comprising gam
US11352611B2 (en) 2017-10-03 2022-06-07 Board Of Regents, The University Of Texas System Non-LTR-retroelement reverse transcriptase and uses thereof
CA3082251A1 (en) 2017-10-16 2019-04-25 The Broad Institute, Inc. Uses of adenosine base editors
EP3724214A4 (en) 2017-12-15 2021-09-01 The Broad Institute Inc. SYSTEMS AND METHODS FOR PREDICTING REPAIR RESULTS IN GENETIC ENGINEERING
WO2019226953A1 (en) 2018-05-23 2019-11-28 The Broad Institute, Inc. Base editors and uses thereof
US12281338B2 (en) 2018-10-29 2025-04-22 The Broad Institute, Inc. Nucleobase editors comprising GeoCas9 and uses thereof
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
DE112020001306T5 (de) 2019-03-19 2022-01-27 Massachusetts Institute Of Technology Verfahren und zusammensetzungen zur editierung von nukleotidsequenzen
US12473543B2 (en) 2019-04-17 2025-11-18 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
CN114258398A (zh) 2019-06-13 2022-03-29 总医院公司 工程化的人内源性病毒样颗粒及使用其递送至细胞的方法
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
WO2021165273A1 (en) * 2020-02-17 2021-08-26 Leadxpro Ag Pro-macrobodies for the enhancement of structure research
AU2021230546A1 (en) 2020-03-04 2022-10-13 Flagship Pioneering Innovations Vi, Llc Methods and compositions for modulating a genome
JP2023525304A (ja) 2020-05-08 2023-06-15 ザ ブロード インスティテュート，インコーポレーテッド 標的二本鎖ヌクレオチド配列の両鎖同時編集のための方法および組成物
BR112023001272A2 (pt) 2020-07-24 2023-04-04 Massachusetts Gen Hospital Partículas semelhantes a vírus aprimoradas e métodos de uso das mesmas para entrega às células
CA3193099A1 (en) 2020-09-24 2022-03-31 David R. Liu Prime editing guide rnas, compositions thereof, and methods of using the same
CA3203876A1 (en) 2021-01-11 2022-07-14 David R. Liu Prime editor variants, constructs, and methods for enhancing prime editing efficiency and precision
WO2023015309A2 (en) 2021-08-06 2023-02-09 The Broad Institute, Inc. Improved prime editors and methods of use
CA3231677A1 (en) 2021-09-08 2023-03-16 Flagship Pioneering Innovations Vi, Llc Methods and compositions for modulating a genome
US20240417719A1 (en) 2021-10-25 2024-12-19 The Broad Institute, Inc. Methods and compositions for editing a genome with prime editing and a recombinase
IL313146A (en) 2021-12-03 2024-07-01 Harvard College Self-assembling virus-like particles for delivery of prime editors and methods of making and using same
WO2023205687A1 (en) 2022-04-20 2023-10-26 The Broad Institute, Inc. Improved prime editing methods and compositions
WO2023240137A1 (en) 2022-06-08 2023-12-14 The Board Institute, Inc. Evolved cas14a1 variants, compositions, and methods of making and using same in genome editing
WO2024077267A1 (en) 2022-10-07 2024-04-11 The Broad Institute, Inc. Prime editing methods and compositions for treating triplet repeat disorders
EP4619515A1 (en) 2022-11-17 2025-09-24 The Broad Institute, Inc. Prime editor delivery by aav
EP4662306A2 (en) 2023-02-09 2025-12-17 The Broad Institute Inc. Evolved recombinases for editing a genome in combination with prime editing
WO2024206125A1 (en) 2023-03-24 2024-10-03 The Broad Institute, Inc. Use of prime editing for treating sickle cell disease
WO2025081042A1 (en) 2023-10-12 2025-04-17 Renagade Therapeutics Management Inc. Nickase-retron template-based precision editing system and methods of use

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5322770A (en) 1989-12-22 1994-06-21 Hoffman-Laroche Inc. Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
US5643758A (en) 1987-03-10 1997-07-01 New England Biolabs, Inc. Production and purification of a protein fused to a binding protein
US5654176A (en) 1987-05-28 1997-08-05 Amrad Corporation Limited Fusion proteins containing glutathione-s-transferase
US5736373A (en) * 1996-09-23 1998-04-07 Becton, Dickinson And Company Thermostable DNA polymerase from Bacillus pallidus
US7001745B1 (en) 1998-09-30 2006-02-21 New England Biolabs, Inc. Intein mediated peptide ligation
US6436677B1 (en) * 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
US6627424B1 (en) * 2000-05-26 2003-09-30 Mj Bioworks, Inc. Nucleic acid modifying enzymes
WO2003008454A2 (en) 2001-07-18 2003-01-30 Merck Patent Gmbh Glycoprotein vi fusion proteins
US7670807B2 (en) * 2004-03-10 2010-03-02 East Tennessee State Univ. Research Foundation RNA-dependent DNA polymerase from Geobacillus stearothermophilus
WO2006020868A2 (en) * 2004-08-13 2006-02-23 New England Biolabs, Inc. Intracellular production of a nuclease

Also Published As

Publication number Publication date
US10150955B2 (en) 2018-12-11
JP2012519489A (ja) 2012-08-30
JP2015193666A (ja) 2015-11-05
AU2010221284B2 (en) 2015-10-01
EP2403941A2 (en) 2012-01-11
US20180340158A1 (en) 2018-11-29
EP2403941B1 (en) 2018-10-03
WO2010102085A4 (en) 2011-01-06
IL214913A0 (en) 2011-11-30
MX2011009205A (es) 2011-09-30
AU2010221284A1 (en) 2011-10-27
ZA201107207B (en) 2012-12-27
WO2010102085A3 (en) 2010-11-18
WO2010102085A2 (en) 2010-09-10
US10113156B2 (en) 2018-10-30
US20180346890A1 (en) 2018-12-06
CN102421791A (zh) 2012-04-18
US10858636B2 (en) 2020-12-08
EP3415621A1 (en) 2018-12-19
IL214913A (en) 2017-06-29
EP2403941A4 (en) 2012-09-05
US20120009630A1 (en) 2012-01-12
US20160289652A1 (en) 2016-10-06
CA2754476A1 (en) 2010-09-10
BRPI1009221A2 (pt) 2016-03-15

Similar Documents

Publication Publication Date Title
JP5882064B2 (ja) 安定化された逆転写酵素融合タンパク質
US7670807B2 (en) RNA-dependent DNA polymerase from Geobacillus stearothermophilus
JP4585996B2 (ja) 弱化３’−５’エキソヌクレアーゼ活性を有する熱安定性又は熱活性ｄｎａポリメラーゼ
EP3556854A1 (en) Heat-resistant reverse transcriptase mutant
KR102880295B1 (ko) Rna로부터의 핵산 증폭반응에 적합한 dna 폴리머라아제 돌연변이체
JP5308027B2 (ja) 変異型ｐｃｎａ
JP7363063B2 (ja) 変異型ｄｎａポリメラーゼ
JP6720632B2 (ja) 融合タンパク質
JP2018161129A (ja) 核酸増幅方法
HK1169130A (en) Stabilized reverse transcriptase fusion proteins
JP6493209B2 (ja) 核酸増幅法
WO2007046556A1 (ja) 新規dnaポリメラーゼ
CN119923465A (zh) 工程化dna聚合酶变体
JP2006197947A (ja) エキソヌクレアーゼ活性が低減された耐熱性ｄｎａポリメラーゼおよびその用途
JP2010183842A (ja) 新規耐熱性ｄｎａポリメラーゼ

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20130128

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20130128

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20140523

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20140616

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20140912

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20140922

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20141016

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20141016

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20150402

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20150803

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20150917

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20151201

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20151225

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20160125

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20160203

R150 Certificate of patent or registration of utility model

Ref document number: 5882064

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250