MX2011009205A - Proteinas de fusion de transcriptasa inversa estabilizada. - Google Patents

Proteinas de fusion de transcriptasa inversa estabilizada.

Info

Publication number
MX2011009205A
MX2011009205A MX2011009205A MX2011009205A MX2011009205A MX 2011009205 A MX2011009205 A MX 2011009205A MX 2011009205 A MX2011009205 A MX 2011009205A MX 2011009205 A MX2011009205 A MX 2011009205A MX 2011009205 A MX2011009205 A MX 2011009205A
Authority
MX
Mexico
Prior art keywords
reverse transcriptase
fusion proteins
protein
stabilized
stabilized reverse
Prior art date
Application number
MX2011009205A
Other languages
English (en)
Inventor
Alan M Lambowitz
Sabine Mohr
Georg Mohr
Eman Ghanem
Original Assignee
Univ Texas
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Texas filed Critical Univ Texas
Publication of MX2011009205A publication Critical patent/MX2011009205A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/24—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a MBP (maltose binding protein)-tag

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Se describen proteínas de fusión de transcriptasa inversa estabilizada incluyendo una transcriptasa inversa termoestable conectada a una proteína estabilizadora. Fijar la proteína estabilizadora a la transcriptasa inversa termoestable estabiliza la proteína de fusión y puede ayudar en su purificación, proveer solubilidad aumentada, permitir almacenamiento más largo, o permitir a la proteína de fusión usarse bajo condiciones más rigurosas tal como temperatura más alta. La proteína de fusión de transcriptasa inversa estabilizada también puede incluir un enlazador entre la proteína estabilizadora y la transcriptasa inversa termoestable. Las proteínas de fusión de transcriptasa inversa estabilizada son adecuadas para usarse en métodos de amplificación de ácido nucleico tal como reacción en cadena de polimerasa de transcripción inversa y otras aplicaciones que involucran síntesis de cADN.
MX2011009205A 2009-03-04 2010-03-04 Proteinas de fusion de transcriptasa inversa estabilizada. MX2011009205A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15733209P 2009-03-04 2009-03-04
PCT/US2010/026165 WO2010102085A2 (en) 2009-03-04 2010-03-04 Stabilized reverse transcriptase fusion proteins

Publications (1)

Publication Number Publication Date
MX2011009205A true MX2011009205A (es) 2011-09-30

Family

ID=42710216

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2011009205A MX2011009205A (es) 2009-03-04 2010-03-04 Proteinas de fusion de transcriptasa inversa estabilizada.

Country Status (11)

Country Link
US (4) US10113156B2 (es)
EP (2) EP3415621A1 (es)
JP (2) JP5882064B2 (es)
CN (1) CN102421791A (es)
AU (1) AU2010221284B2 (es)
BR (1) BRPI1009221A2 (es)
CA (1) CA2754476A1 (es)
IL (1) IL214913A (es)
MX (1) MX2011009205A (es)
WO (1) WO2010102085A2 (es)
ZA (1) ZA201107207B (es)

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KR101818126B1 (ko) * 2011-02-09 2018-01-15 (주)바이오니아 열안정성이 증가된 역전사효소
WO2012116146A1 (en) * 2011-02-23 2012-08-30 Board Of Regents Use of template switching for dna synthesis
IL310721A (en) 2015-10-23 2024-04-01 Harvard College Nucleobase editors and their uses
WO2017100177A1 (en) * 2015-12-07 2017-06-15 Bio-Rad Laboratories, Inc. Dimeric reverse transcriptase
CA3032699A1 (en) 2016-08-03 2018-02-08 President And Fellows Of Harvard College Adenosine nucleobase editors and uses thereof
AU2017308889B2 (en) 2016-08-09 2023-11-09 President And Fellows Of Harvard College Programmable Cas9-recombinase fusion proteins and uses thereof
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
CN110214180A (zh) 2016-10-14 2019-09-06 哈佛大学的校长及成员们 核碱基编辑器的aav递送
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
EP3592853A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression of pain by gene editing
US11542496B2 (en) 2017-03-10 2023-01-03 President And Fellows Of Harvard College Cytosine to guanine base editor
CN110914426A (zh) 2017-03-23 2020-03-24 哈佛大学的校长及成员们 包含核酸可编程dna结合蛋白的核碱基编辑器
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
CN111801345A (zh) 2017-07-28 2020-10-20 哈佛大学的校长及成员们 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物
US11319532B2 (en) 2017-08-30 2022-05-03 President And Fellows Of Harvard College High efficiency base editors comprising Gam
WO2019070843A1 (en) * 2017-10-03 2019-04-11 Board Of Regents, The University Of Texas System INVERSE TRANSCRIPTASE WITH LTR-FREE BACKLIGHTS AND USES THEREOF
AU2018352592A1 (en) 2017-10-16 2020-06-04 Beam Therapeutics, Inc. Uses of adenosine base editors
AU2020242032A1 (en) 2019-03-19 2021-10-07 Massachusetts Institute Of Technology Methods and compositions for editing nucleotide sequences
CA3177481A1 (en) 2020-05-08 2021-11-11 David R. Liu Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence
JP2023543803A (ja) 2020-09-24 2023-10-18 ザ ブロード インスティテュート，インコーポレーテッド プライム編集ガイドｒｎａ、その組成物、及びその使用方法
WO2022150790A2 (en) 2021-01-11 2022-07-14 The Broad Institute, Inc. Prime editor variants, constructs, and methods for enhancing prime editing efficiency and precision
WO2023015309A2 (en) 2021-08-06 2023-02-09 The Broad Institute, Inc. Improved prime editors and methods of use
WO2023076898A1 (en) 2021-10-25 2023-05-04 The Broad Institute, Inc. Methods and compositions for editing a genome with prime editing and a recombinase
WO2023102538A1 (en) 2021-12-03 2023-06-08 The Broad Institute, Inc. Self-assembling virus-like particles for delivery of prime editors and methods of making and using same
WO2023205687A1 (en) 2022-04-20 2023-10-26 The Broad Institute, Inc. Improved prime editing methods and compositions
WO2023240137A1 (en) 2022-06-08 2023-12-14 The Board Institute, Inc. Evolved cas14a1 variants, compositions, and methods of making and using same in genome editing
WO2024077267A1 (en) 2022-10-07 2024-04-11 The Broad Institute, Inc. Prime editing methods and compositions for treating triplet repeat disorders
WO2024108092A1 (en) 2022-11-17 2024-05-23 The Broad Institute, Inc. Prime editor delivery by aav

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US5322770A (en) 1989-12-22 1994-06-21 Hoffman-Laroche Inc. Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
US5643758A (en) 1987-03-10 1997-07-01 New England Biolabs, Inc. Production and purification of a protein fused to a binding protein
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Also Published As

Publication number Publication date
US20120009630A1 (en) 2012-01-12
EP2403941B1 (en) 2018-10-03
JP5882064B2 (ja) 2016-03-09
JP2015193666A (ja) 2015-11-05
US20180340158A1 (en) 2018-11-29
US20160289652A1 (en) 2016-10-06
EP2403941A4 (en) 2012-09-05
CN102421791A (zh) 2012-04-18
BRPI1009221A2 (pt) 2016-03-15
JP2012519489A (ja) 2012-08-30
CA2754476A1 (en) 2010-09-10
AU2010221284B2 (en) 2015-10-01
WO2010102085A4 (en) 2011-01-06
US10113156B2 (en) 2018-10-30
US10150955B2 (en) 2018-12-11
IL214913A0 (en) 2011-11-30
IL214913A (en) 2017-06-29
ZA201107207B (en) 2012-12-27
WO2010102085A2 (en) 2010-09-10
US20180346890A1 (en) 2018-12-06
WO2010102085A3 (en) 2010-11-18
AU2010221284A1 (en) 2011-10-27
EP2403941A2 (en) 2012-01-11
EP3415621A1 (en) 2018-12-19
US10858636B2 (en) 2020-12-08

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