JP2012519489A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2012519489A5 JP2012519489A5 JP2011553101A JP2011553101A JP2012519489A5 JP 2012519489 A5 JP2012519489 A5 JP 2012519489A5 JP 2011553101 A JP2011553101 A JP 2011553101A JP 2011553101 A JP2011553101 A JP 2011553101A JP 2012519489 A5 JP2012519489 A5 JP 2012519489A5
- Authority
- JP
- Japan
- Prior art keywords
- intron
- group
- tag
- protein
- constructs
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108090000623 proteins and genes Proteins 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 108091029499 Group II intron Proteins 0.000 description 3
- 210000004899 c-terminal region Anatomy 0.000 description 2
- 239000013612 plasmid Substances 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 229920000856 Amylose Polymers 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
- 108091092195 Intron Proteins 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 108020001507 fusion proteins Proteins 0.000 description 1
- 102000037865 fusion proteins Human genes 0.000 description 1
- 229960002897 heparin Drugs 0.000 description 1
- 229920000669 heparin Polymers 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US15733209P | 2009-03-04 | 2009-03-04 | |
| US61/157,332 | 2009-03-04 | ||
| PCT/US2010/026165 WO2010102085A2 (en) | 2009-03-04 | 2010-03-04 | Stabilized reverse transcriptase fusion proteins |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015153175A Division JP2015193666A (ja) | 2009-03-04 | 2015-08-03 | 安定化された逆転写酵素融合タンパク質 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2012519489A JP2012519489A (ja) | 2012-08-30 |
| JP2012519489A5 true JP2012519489A5 (enExample) | 2013-03-14 |
| JP5882064B2 JP5882064B2 (ja) | 2016-03-09 |
Family
ID=42710216
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011553101A Active JP5882064B2 (ja) | 2009-03-04 | 2010-03-04 | 安定化された逆転写酵素融合タンパク質 |
| JP2015153175A Withdrawn JP2015193666A (ja) | 2009-03-04 | 2015-08-03 | 安定化された逆転写酵素融合タンパク質 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015153175A Withdrawn JP2015193666A (ja) | 2009-03-04 | 2015-08-03 | 安定化された逆転写酵素融合タンパク質 |
Country Status (11)
| Country | Link |
|---|---|
| US (4) | US10113156B2 (enExample) |
| EP (2) | EP2403941B1 (enExample) |
| JP (2) | JP5882064B2 (enExample) |
| CN (1) | CN102421791A (enExample) |
| AU (1) | AU2010221284B2 (enExample) |
| BR (1) | BRPI1009221A2 (enExample) |
| CA (1) | CA2754476A1 (enExample) |
| IL (1) | IL214913A (enExample) |
| MX (1) | MX2011009205A (enExample) |
| WO (1) | WO2010102085A2 (enExample) |
| ZA (1) | ZA201107207B (enExample) |
Families Citing this family (44)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101818126B1 (ko) * | 2011-02-09 | 2018-01-15 | (주)바이오니아 | 열안정성이 증가된 역전사효소 |
| US9012183B2 (en) | 2011-02-23 | 2015-04-21 | Board Of Regents, The University Of Texas System | Use of template switching for DNA synthesis |
| US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
| US12043852B2 (en) | 2015-10-23 | 2024-07-23 | President And Fellows Of Harvard College | Evolved Cas9 proteins for gene editing |
| EP3387002A4 (en) | 2015-12-07 | 2019-04-24 | Bio-Rad Laboratories, Inc. | DIM REVERSE TRANSCRIPTASE |
| KR20250103795A (ko) | 2016-08-03 | 2025-07-07 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 아데노신 핵염기 편집제 및 그의 용도 |
| CN109804066A (zh) | 2016-08-09 | 2019-05-24 | 哈佛大学的校长及成员们 | 可编程cas9-重组酶融合蛋白及其用途 |
| US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| AU2017342543B2 (en) | 2016-10-14 | 2024-06-27 | President And Fellows Of Harvard College | AAV delivery of nucleobase editors |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| EP3592381A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Cancer vaccine |
| EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
| KR20190127797A (ko) | 2017-03-10 | 2019-11-13 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 시토신에서 구아닌으로의 염기 편집제 |
| CA3057192A1 (en) | 2017-03-23 | 2018-09-27 | President And Fellows Of Harvard College | Nucleobase editors comprising nucleic acid programmable dna binding proteins |
| WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
| CN111801345A (zh) | 2017-07-28 | 2020-10-20 | 哈佛大学的校长及成员们 | 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物 |
| WO2019139645A2 (en) | 2017-08-30 | 2019-07-18 | President And Fellows Of Harvard College | High efficiency base editors comprising gam |
| US11352611B2 (en) | 2017-10-03 | 2022-06-07 | Board Of Regents, The University Of Texas System | Non-LTR-retroelement reverse transcriptase and uses thereof |
| CA3082251A1 (en) | 2017-10-16 | 2019-04-25 | The Broad Institute, Inc. | Uses of adenosine base editors |
| EP3724214A4 (en) | 2017-12-15 | 2021-09-01 | The Broad Institute Inc. | SYSTEMS AND METHODS FOR PREDICTING REPAIR RESULTS IN GENETIC ENGINEERING |
| WO2019226953A1 (en) | 2018-05-23 | 2019-11-28 | The Broad Institute, Inc. | Base editors and uses thereof |
| US12281338B2 (en) | 2018-10-29 | 2025-04-22 | The Broad Institute, Inc. | Nucleobase editors comprising GeoCas9 and uses thereof |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| DE112020001306T5 (de) | 2019-03-19 | 2022-01-27 | Massachusetts Institute Of Technology | Verfahren und zusammensetzungen zur editierung von nukleotidsequenzen |
| US12473543B2 (en) | 2019-04-17 | 2025-11-18 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| CN114258398A (zh) | 2019-06-13 | 2022-03-29 | 总医院公司 | 工程化的人内源性病毒样颗粒及使用其递送至细胞的方法 |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| WO2021165273A1 (en) * | 2020-02-17 | 2021-08-26 | Leadxpro Ag | Pro-macrobodies for the enhancement of structure research |
| AU2021230546A1 (en) | 2020-03-04 | 2022-10-13 | Flagship Pioneering Innovations Vi, Llc | Methods and compositions for modulating a genome |
| JP2023525304A (ja) | 2020-05-08 | 2023-06-15 | ザ ブロード インスティテュート，インコーポレーテッド | 標的二本鎖ヌクレオチド配列の両鎖同時編集のための方法および組成物 |
| BR112023001272A2 (pt) | 2020-07-24 | 2023-04-04 | Massachusetts Gen Hospital | Partículas semelhantes a vírus aprimoradas e métodos de uso das mesmas para entrega às células |
| CA3193099A1 (en) | 2020-09-24 | 2022-03-31 | David R. Liu | Prime editing guide rnas, compositions thereof, and methods of using the same |
| CA3203876A1 (en) | 2021-01-11 | 2022-07-14 | David R. Liu | Prime editor variants, constructs, and methods for enhancing prime editing efficiency and precision |
| WO2023015309A2 (en) | 2021-08-06 | 2023-02-09 | The Broad Institute, Inc. | Improved prime editors and methods of use |
| CA3231677A1 (en) | 2021-09-08 | 2023-03-16 | Flagship Pioneering Innovations Vi, Llc | Methods and compositions for modulating a genome |
| US20240417719A1 (en) | 2021-10-25 | 2024-12-19 | The Broad Institute, Inc. | Methods and compositions for editing a genome with prime editing and a recombinase |
| IL313146A (en) | 2021-12-03 | 2024-07-01 | Harvard College | Self-assembling virus-like particles for delivery of prime editors and methods of making and using same |
| WO2023205687A1 (en) | 2022-04-20 | 2023-10-26 | The Broad Institute, Inc. | Improved prime editing methods and compositions |
| WO2023240137A1 (en) | 2022-06-08 | 2023-12-14 | The Board Institute, Inc. | Evolved cas14a1 variants, compositions, and methods of making and using same in genome editing |
| WO2024077267A1 (en) | 2022-10-07 | 2024-04-11 | The Broad Institute, Inc. | Prime editing methods and compositions for treating triplet repeat disorders |
| EP4619515A1 (en) | 2022-11-17 | 2025-09-24 | The Broad Institute, Inc. | Prime editor delivery by aav |
| EP4662306A2 (en) | 2023-02-09 | 2025-12-17 | The Broad Institute Inc. | Evolved recombinases for editing a genome in combination with prime editing |
| WO2024206125A1 (en) | 2023-03-24 | 2024-10-03 | The Broad Institute, Inc. | Use of prime editing for treating sickle cell disease |
| WO2025081042A1 (en) | 2023-10-12 | 2025-04-17 | Renagade Therapeutics Management Inc. | Nickase-retron template-based precision editing system and methods of use |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5322770A (en) | 1989-12-22 | 1994-06-21 | Hoffman-Laroche Inc. | Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription |
| US5643758A (en) | 1987-03-10 | 1997-07-01 | New England Biolabs, Inc. | Production and purification of a protein fused to a binding protein |
| US5654176A (en) | 1987-05-28 | 1997-08-05 | Amrad Corporation Limited | Fusion proteins containing glutathione-s-transferase |
| US5736373A (en) * | 1996-09-23 | 1998-04-07 | Becton, Dickinson And Company | Thermostable DNA polymerase from Bacillus pallidus |
| US7001745B1 (en) | 1998-09-30 | 2006-02-21 | New England Biolabs, Inc. | Intein mediated peptide ligation |
| US6436677B1 (en) * | 2000-03-02 | 2002-08-20 | Promega Corporation | Method of reverse transcription |
| US6627424B1 (en) * | 2000-05-26 | 2003-09-30 | Mj Bioworks, Inc. | Nucleic acid modifying enzymes |
| WO2003008454A2 (en) | 2001-07-18 | 2003-01-30 | Merck Patent Gmbh | Glycoprotein vi fusion proteins |
| US7670807B2 (en) * | 2004-03-10 | 2010-03-02 | East Tennessee State Univ. Research Foundation | RNA-dependent DNA polymerase from Geobacillus stearothermophilus |
| WO2006020868A2 (en) * | 2004-08-13 | 2006-02-23 | New England Biolabs, Inc. | Intracellular production of a nuclease |
-
2010
- 2010-03-04 MX MX2011009205A patent/MX2011009205A/es not_active Application Discontinuation
- 2010-03-04 AU AU2010221284A patent/AU2010221284B2/en not_active Ceased
- 2010-03-04 CN CN2010800201231A patent/CN102421791A/zh active Pending
- 2010-03-04 CA CA2754476A patent/CA2754476A1/en not_active Abandoned
- 2010-03-04 US US13/254,223 patent/US10113156B2/en active Active
- 2010-03-04 BR BRPI1009221A patent/BRPI1009221A2/pt not_active IP Right Cessation
- 2010-03-04 EP EP10749312.4A patent/EP2403941B1/en active Active
- 2010-03-04 WO PCT/US2010/026165 patent/WO2010102085A2/en not_active Ceased
- 2010-03-04 EP EP18184036.4A patent/EP3415621A1/en not_active Withdrawn
- 2010-03-04 JP JP2011553101A patent/JP5882064B2/ja active Active
-
2011
- 2011-09-01 IL IL214913A patent/IL214913A/en active IP Right Grant
- 2011-10-03 ZA ZA2011/07207A patent/ZA201107207B/en unknown
-
2015
- 2015-08-03 JP JP2015153175A patent/JP2015193666A/ja not_active Withdrawn
-
2016
- 2016-05-25 US US15/164,208 patent/US10150955B2/en active Active
-
2018
- 2018-08-01 US US16/051,549 patent/US10858636B2/en active Active
- 2018-08-01 US US16/051,544 patent/US20180340158A1/en not_active Abandoned
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2012519489A5 (enExample) | ||
| US11472837B2 (en) | Process of manufacture of annexin V | |
| JP7094103B2 (ja) | インフルエンザウイルスワクチンおよびその使用 | |
| JP2022116236A (ja) | Ｂｕｒｋｈｏｌｄｅｒｉａ感染の処置のための組成物および方法 | |
| US20230340031A1 (en) | Self-assembling protein nanoparticles with built-in six-helix bundle proteins | |
| JP2010273685A5 (enExample) | ||
| JP7385680B2 (ja) | 変異型ｒｓｖ ｆタンパク質及びその利用 | |
| JP2010068806A5 (enExample) | ||
| JP2014517835A5 (enExample) | ||
| WO2011157713A4 (en) | Cell-penetrating peptides and uses therof | |
| TW201520221A (zh) | 穩定化可溶性融合前ｒｓｖ ｆ多肽 | |
| JP2015212284A5 (enExample) | ||
| JP2015509707A5 (enExample) | ||
| CN103214563B (zh) | 一种性能改进的重组金黄色葡萄球菌蛋白a亲和配基及其构建方法 | |
| JP7096239B2 (ja) | タンパク質の発現および送達のための組成物および方法 | |
| ES2375863T3 (es) | Prote�?na de fusión quimérica con actividades de chaperón y de plegamiento superiores. | |
| JP2016513471A5 (enExample) | ||
| CN104805104B (zh) | 一种海藻糖合酶及其编码基因与应用 | |
| CN107226842B (zh) | 靶向pd-1的多肽及其应用 | |
| JP2013516991A5 (enExample) | ||
| JPWO2020027010A5 (enExample) | ||
| Orrapin et al. | Recombinant expression of novel protegrin-1 dimer and LL-37-linker–histatin-5 hybrid peptide mediated biotin carboxyl carrier protein fusion partner | |
| JP2009536821A5 (enExample) | ||
| Chhetri et al. | Recombinant expression, purification and preliminary characterization of the mRNA export factor MEX67 of Saccharomyces cerevisiae | |
| CN105542003B (zh) | 一种针对rsv粘附g蛋白表面抗原的全人源单克隆抗体 |