JP2012519489A5 - - Google Patents
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- Publication number
- JP2012519489A5 JP2012519489A5 JP2011553101A JP2011553101A JP2012519489A5 JP 2012519489 A5 JP2012519489 A5 JP 2012519489A5 JP 2011553101 A JP2011553101 A JP 2011553101A JP 2011553101 A JP2011553101 A JP 2011553101A JP 2012519489 A5 JP2012519489 A5 JP 2012519489A5
- Authority
- JP
- Japan
- Prior art keywords
- intron
- group
- tag
- protein
- constructs
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108090000623 proteins and genes Proteins 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 108091029499 Group II intron Proteins 0.000 description 3
- 210000004899 c-terminal region Anatomy 0.000 description 2
- 239000013612 plasmid Substances 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 229920000856 Amylose Polymers 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
- 108091092195 Intron Proteins 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 108020001507 fusion proteins Proteins 0.000 description 1
- 102000037865 fusion proteins Human genes 0.000 description 1
- 229960002897 heparin Drugs 0.000 description 1
- 229920000669 heparin Polymers 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US15733209P | 2009-03-04 | 2009-03-04 | |
| US61/157,332 | 2009-03-04 | ||
| PCT/US2010/026165 WO2010102085A2 (en) | 2009-03-04 | 2010-03-04 | Stabilized reverse transcriptase fusion proteins |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015153175A Division JP2015193666A (ja) | 2009-03-04 | 2015-08-03 | 安定化された逆転写酵素融合タンパク質 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2012519489A JP2012519489A (ja) | 2012-08-30 |
| JP2012519489A5 true JP2012519489A5 (enExample) | 2013-03-14 |
| JP5882064B2 JP5882064B2 (ja) | 2016-03-09 |
Family
ID=42710216
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011553101A Active JP5882064B2 (ja) | 2009-03-04 | 2010-03-04 | 安定化された逆転写酵素融合タンパク質 |
| JP2015153175A Withdrawn JP2015193666A (ja) | 2009-03-04 | 2015-08-03 | 安定化された逆転写酵素融合タンパク質 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015153175A Withdrawn JP2015193666A (ja) | 2009-03-04 | 2015-08-03 | 安定化された逆転写酵素融合タンパク質 |
Country Status (11)
| Country | Link |
|---|---|
| US (4) | US10113156B2 (enExample) |
| EP (2) | EP3415621A1 (enExample) |
| JP (2) | JP5882064B2 (enExample) |
| CN (1) | CN102421791A (enExample) |
| AU (1) | AU2010221284B2 (enExample) |
| BR (1) | BRPI1009221A2 (enExample) |
| CA (1) | CA2754476A1 (enExample) |
| IL (1) | IL214913A (enExample) |
| MX (1) | MX2011009205A (enExample) |
| WO (1) | WO2010102085A2 (enExample) |
| ZA (1) | ZA201107207B (enExample) |
Families Citing this family (50)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101818126B1 (ko) * | 2011-02-09 | 2018-01-15 | (주)바이오니아 | 열안정성이 증가된 역전사효소 |
| KR101911969B1 (ko) * | 2011-02-23 | 2018-10-25 | 보드 오브 리전츠, 더 유니버시티 오브 텍사스 시스템 | Ｄｎａ 합성을 위한 주형 스위칭의 용도 |
| US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| WO2017070632A2 (en) | 2015-10-23 | 2017-04-27 | President And Fellows Of Harvard College | Nucleobase editors and uses thereof |
| WO2017100177A1 (en) | 2015-12-07 | 2017-06-15 | Bio-Rad Laboratories, Inc. | Dimeric reverse transcriptase |
| WO2018027078A1 (en) | 2016-08-03 | 2018-02-08 | President And Fellows Of Harard College | Adenosine nucleobase editors and uses thereof |
| AU2017308889B2 (en) | 2016-08-09 | 2023-11-09 | President And Fellows Of Harvard College | Programmable Cas9-recombinase fusion proteins and uses thereof |
| WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| KR20240007715A (ko) | 2016-10-14 | 2024-01-16 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵염기 에디터의 aav 전달 |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| KR20190123328A (ko) | 2017-03-09 | 2019-10-31 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 암 백신 |
| WO2018165504A1 (en) | 2017-03-09 | 2018-09-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
| CN110914310A (zh) | 2017-03-10 | 2020-03-24 | 哈佛大学的校长及成员们 | 胞嘧啶至鸟嘌呤碱基编辑器 |
| IL306092A (en) | 2017-03-23 | 2023-11-01 | Harvard College | Nucleic base editors that include nucleic acid programmable DNA binding proteins |
| US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
| EP3658573A1 (en) | 2017-07-28 | 2020-06-03 | President and Fellows of Harvard College | Methods and compositions for evolving base editors using phage-assisted continuous evolution (pace) |
| EP3676376B1 (en) | 2017-08-30 | 2025-01-15 | President and Fellows of Harvard College | High efficiency base editors comprising gam |
| WO2019070843A1 (en) * | 2017-10-03 | 2019-04-11 | Board Of Regents, The University Of Texas System | INVERSE TRANSCRIPTASE WITH LTR-FREE BACKLIGHTS AND USES THEREOF |
| CA3082251A1 (en) | 2017-10-16 | 2019-04-25 | The Broad Institute, Inc. | Uses of adenosine base editors |
| US12406749B2 (en) | 2017-12-15 | 2025-09-02 | The Broad Institute, Inc. | Systems and methods for predicting repair outcomes in genetic engineering |
| WO2019226953A1 (en) | 2018-05-23 | 2019-11-28 | The Broad Institute, Inc. | Base editors and uses thereof |
| EP3820495A4 (en) | 2018-07-09 | 2022-07-20 | The Broad Institute Inc. | RNA-PROGRAMMABLE EPIGENETIC RNA MODIFIERS AND THEIR USES |
| JP2021533751A (ja) * | 2018-08-08 | 2021-12-09 | ザ リージェンツ オブ ザ ユニヴァーシティ オブ カリフォルニアThe Regents of the University of California | 不連続な複数の鋳型から相補的ＤＮＡ（ｃＤＮＡ）を順序だてて連続的に合成するための組成物およびその方法 |
| WO2020092453A1 (en) | 2018-10-29 | 2020-05-07 | The Broad Institute, Inc. | Nucleobase editors comprising geocas9 and uses thereof |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| WO2020191241A1 (en) | 2019-03-19 | 2020-09-24 | The Broad Institute, Inc. | Methods and compositions for editing nucleotide sequences |
| WO2020214842A1 (en) | 2019-04-17 | 2020-10-22 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| US20220259617A1 (en) | 2019-06-13 | 2022-08-18 | The General Hospital Corporation | Engineered human-endogenous virus-like particles and methods of use thereof for delivery to cells |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| JP7839094B2 (ja) * | 2020-02-17 | 2026-04-01 | リーデクスプロ アーゲー | 構造研究の促進のためのｐｒｏ－マクロボディ |
| BR112022017715A2 (pt) | 2020-03-04 | 2022-11-16 | Flagship Pioneering Innovations Vi Llc | Métodos e composições para modular um genoma |
| CA3177481A1 (en) | 2020-05-08 | 2021-11-11 | David R. Liu | Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence |
| BR112023001272A2 (pt) | 2020-07-24 | 2023-04-04 | Massachusetts Gen Hospital | Partículas semelhantes a vírus aprimoradas e métodos de uso das mesmas para entrega às células |
| CA3193099A1 (en) | 2020-09-24 | 2022-03-31 | David R. Liu | Prime editing guide rnas, compositions thereof, and methods of using the same |
| AU2022206476A1 (en) | 2021-01-11 | 2023-07-20 | President And Fellows Of Harvard College | Prime editor variants, constructs, and methods for enhancing prime editing efficiency and precision |
| AU2022325166A1 (en) | 2021-08-06 | 2024-02-08 | President And Fellows Of Harvard College | Improved prime editors and methods of use |
| US20230141630A1 (en) * | 2021-08-17 | 2023-05-11 | New England Biolabs, Inc. | Rolling Circle Reverse Transcription of Circular RNA |
| KR20260037664A (ko) | 2021-09-08 | 2026-03-18 | 플래그쉽 파이어니어링 이노베이션스 브이아이, 엘엘씨 | 게놈을 조절하기 위한 방법 및 조성물 |
| US20240417719A1 (en) | 2021-10-25 | 2024-12-19 | The Broad Institute, Inc. | Methods and compositions for editing a genome with prime editing and a recombinase |
| IL313146A (en) | 2021-12-03 | 2024-07-01 | Harvard College | Self-assembling virus-like particles for delivery of prime editors and methods of making and using same |
| WO2023205687A1 (en) | 2022-04-20 | 2023-10-26 | The Broad Institute, Inc. | Improved prime editing methods and compositions |
| WO2023240137A1 (en) | 2022-06-08 | 2023-12-14 | The Board Institute, Inc. | Evolved cas14a1 variants, compositions, and methods of making and using same in genome editing |
| WO2024077267A1 (en) | 2022-10-07 | 2024-04-11 | The Broad Institute, Inc. | Prime editing methods and compositions for treating triplet repeat disorders |
| CN116814581A (zh) * | 2022-10-13 | 2023-09-29 | 苏州拜科生物科技有限公司 | 一种热稳定性反转录酶突变体基因、制备方法和应用 |
| WO2024108092A1 (en) | 2022-11-17 | 2024-05-23 | The Broad Institute, Inc. | Prime editor delivery by aav |
| EP4662306A2 (en) | 2023-02-09 | 2025-12-17 | The Broad Institute Inc. | Evolved recombinases for editing a genome in combination with prime editing |
| WO2024206125A1 (en) | 2023-03-24 | 2024-10-03 | The Broad Institute, Inc. | Use of prime editing for treating sickle cell disease |
| WO2025081042A1 (en) | 2023-10-12 | 2025-04-17 | Renagade Therapeutics Management Inc. | Nickase-retron template-based precision editing system and methods of use |
| CN121086027B (zh) * | 2025-09-03 | 2026-07-28 | 湖南艾科瑞生物工程有限公司 | 一种mmlv逆转录酶的多肽配体及其应用 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5322770A (en) | 1989-12-22 | 1994-06-21 | Hoffman-Laroche Inc. | Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription |
| US5643758A (en) | 1987-03-10 | 1997-07-01 | New England Biolabs, Inc. | Production and purification of a protein fused to a binding protein |
| US5654176A (en) | 1987-05-28 | 1997-08-05 | Amrad Corporation Limited | Fusion proteins containing glutathione-s-transferase |
| US5736373A (en) * | 1996-09-23 | 1998-04-07 | Becton, Dickinson And Company | Thermostable DNA polymerase from Bacillus pallidus |
| US7001745B1 (en) | 1998-09-30 | 2006-02-21 | New England Biolabs, Inc. | Intein mediated peptide ligation |
| US6436677B1 (en) | 2000-03-02 | 2002-08-20 | Promega Corporation | Method of reverse transcription |
| US6627424B1 (en) * | 2000-05-26 | 2003-09-30 | Mj Bioworks, Inc. | Nucleic acid modifying enzymes |
| WO2003008454A2 (en) * | 2001-07-18 | 2003-01-30 | Merck Patent Gmbh | Glycoprotein vi fusion proteins |
| US7670807B2 (en) * | 2004-03-10 | 2010-03-02 | East Tennessee State Univ. Research Foundation | RNA-dependent DNA polymerase from Geobacillus stearothermophilus |
| US7935509B2 (en) * | 2004-08-13 | 2011-05-03 | New England Biolabs, Inc. | Intracellular production of a nuclease |
-
2010
- 2010-03-04 JP JP2011553101A patent/JP5882064B2/ja active Active
- 2010-03-04 EP EP18184036.4A patent/EP3415621A1/en not_active Withdrawn
- 2010-03-04 BR BRPI1009221A patent/BRPI1009221A2/pt not_active IP Right Cessation
- 2010-03-04 MX MX2011009205A patent/MX2011009205A/es not_active Application Discontinuation
- 2010-03-04 CA CA2754476A patent/CA2754476A1/en not_active Abandoned
- 2010-03-04 EP EP10749312.4A patent/EP2403941B1/en active Active
- 2010-03-04 AU AU2010221284A patent/AU2010221284B2/en not_active Ceased
- 2010-03-04 CN CN2010800201231A patent/CN102421791A/zh active Pending
- 2010-03-04 US US13/254,223 patent/US10113156B2/en active Active
- 2010-03-04 WO PCT/US2010/026165 patent/WO2010102085A2/en not_active Ceased
-
2011
- 2011-09-01 IL IL214913A patent/IL214913A/en active IP Right Grant
- 2011-10-03 ZA ZA2011/07207A patent/ZA201107207B/en unknown
-
2015
- 2015-08-03 JP JP2015153175A patent/JP2015193666A/ja not_active Withdrawn
-
2016
- 2016-05-25 US US15/164,208 patent/US10150955B2/en active Active
-
2018
- 2018-08-01 US US16/051,544 patent/US20180340158A1/en not_active Abandoned
- 2018-08-01 US US16/051,549 patent/US10858636B2/en active Active
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