CA2754476A1 - Stabilized reverse transcriptase fusion proteins - Google Patents
Stabilized reverse transcriptase fusion proteins Download PDFInfo
- Publication number
- CA2754476A1 CA2754476A1 CA2754476A CA2754476A CA2754476A1 CA 2754476 A1 CA2754476 A1 CA 2754476A1 CA 2754476 A CA2754476 A CA 2754476A CA 2754476 A CA2754476 A CA 2754476A CA 2754476 A1 CA2754476 A1 CA 2754476A1
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- Prior art keywords
- reverse transcriptase
- seq
- protein
- fusion protein
- stabilized
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/24—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a MBP (maltose binding protein)-tag
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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| Application Number | Priority Date | Filing Date | Title |
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| US15733209P | 2009-03-04 | 2009-03-04 | |
| US61/157,332 | 2009-03-04 | ||
| PCT/US2010/026165 WO2010102085A2 (en) | 2009-03-04 | 2010-03-04 | Stabilized reverse transcriptase fusion proteins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2754476A1 true CA2754476A1 (en) | 2010-09-10 |
Family
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|---|---|---|---|
| CA2754476A Abandoned CA2754476A1 (en) | 2009-03-04 | 2010-03-04 | Stabilized reverse transcriptase fusion proteins |
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|---|---|
| US (4) | US10113156B2 (enExample) |
| EP (2) | EP2403941B1 (enExample) |
| JP (2) | JP5882064B2 (enExample) |
| CN (1) | CN102421791A (enExample) |
| AU (1) | AU2010221284B2 (enExample) |
| BR (1) | BRPI1009221A2 (enExample) |
| CA (1) | CA2754476A1 (enExample) |
| IL (1) | IL214913A (enExample) |
| MX (1) | MX2011009205A (enExample) |
| WO (1) | WO2010102085A2 (enExample) |
| ZA (1) | ZA201107207B (enExample) |
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| KR101818126B1 (ko) * | 2011-02-09 | 2018-01-15 | (주)바이오니아 | 열안정성이 증가된 역전사효소 |
| US9012183B2 (en) | 2011-02-23 | 2015-04-21 | Board Of Regents, The University Of Texas System | Use of template switching for DNA synthesis |
| US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
| US12043852B2 (en) | 2015-10-23 | 2024-07-23 | President And Fellows Of Harvard College | Evolved Cas9 proteins for gene editing |
| EP3387002A4 (en) | 2015-12-07 | 2019-04-24 | Bio-Rad Laboratories, Inc. | DIM REVERSE TRANSCRIPTASE |
| KR20250103795A (ko) | 2016-08-03 | 2025-07-07 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 아데노신 핵염기 편집제 및 그의 용도 |
| CN109804066A (zh) | 2016-08-09 | 2019-05-24 | 哈佛大学的校长及成员们 | 可编程cas9-重组酶融合蛋白及其用途 |
| US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| AU2017342543B2 (en) | 2016-10-14 | 2024-06-27 | President And Fellows Of Harvard College | AAV delivery of nucleobase editors |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| EP3592381A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Cancer vaccine |
| EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
| KR20190127797A (ko) | 2017-03-10 | 2019-11-13 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 시토신에서 구아닌으로의 염기 편집제 |
| CA3057192A1 (en) | 2017-03-23 | 2018-09-27 | President And Fellows Of Harvard College | Nucleobase editors comprising nucleic acid programmable dna binding proteins |
| WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
| CN111801345A (zh) | 2017-07-28 | 2020-10-20 | 哈佛大学的校长及成员们 | 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物 |
| WO2019139645A2 (en) | 2017-08-30 | 2019-07-18 | President And Fellows Of Harvard College | High efficiency base editors comprising gam |
| US11352611B2 (en) | 2017-10-03 | 2022-06-07 | Board Of Regents, The University Of Texas System | Non-LTR-retroelement reverse transcriptase and uses thereof |
| CA3082251A1 (en) | 2017-10-16 | 2019-04-25 | The Broad Institute, Inc. | Uses of adenosine base editors |
| EP3724214A4 (en) | 2017-12-15 | 2021-09-01 | The Broad Institute Inc. | SYSTEMS AND METHODS FOR PREDICTING REPAIR RESULTS IN GENETIC ENGINEERING |
| WO2019226953A1 (en) | 2018-05-23 | 2019-11-28 | The Broad Institute, Inc. | Base editors and uses thereof |
| US12281338B2 (en) | 2018-10-29 | 2025-04-22 | The Broad Institute, Inc. | Nucleobase editors comprising GeoCas9 and uses thereof |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| DE112020001306T5 (de) | 2019-03-19 | 2022-01-27 | Massachusetts Institute Of Technology | Verfahren und zusammensetzungen zur editierung von nukleotidsequenzen |
| US12473543B2 (en) | 2019-04-17 | 2025-11-18 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| CN114258398A (zh) | 2019-06-13 | 2022-03-29 | 总医院公司 | 工程化的人内源性病毒样颗粒及使用其递送至细胞的方法 |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| WO2021165273A1 (en) * | 2020-02-17 | 2021-08-26 | Leadxpro Ag | Pro-macrobodies for the enhancement of structure research |
| AU2021230546A1 (en) | 2020-03-04 | 2022-10-13 | Flagship Pioneering Innovations Vi, Llc | Methods and compositions for modulating a genome |
| JP2023525304A (ja) | 2020-05-08 | 2023-06-15 | ザ ブロード インスティテュート，インコーポレーテッド | 標的二本鎖ヌクレオチド配列の両鎖同時編集のための方法および組成物 |
| BR112023001272A2 (pt) | 2020-07-24 | 2023-04-04 | Massachusetts Gen Hospital | Partículas semelhantes a vírus aprimoradas e métodos de uso das mesmas para entrega às células |
| CA3193099A1 (en) | 2020-09-24 | 2022-03-31 | David R. Liu | Prime editing guide rnas, compositions thereof, and methods of using the same |
| CA3203876A1 (en) | 2021-01-11 | 2022-07-14 | David R. Liu | Prime editor variants, constructs, and methods for enhancing prime editing efficiency and precision |
| WO2023015309A2 (en) | 2021-08-06 | 2023-02-09 | The Broad Institute, Inc. | Improved prime editors and methods of use |
| CA3231677A1 (en) | 2021-09-08 | 2023-03-16 | Flagship Pioneering Innovations Vi, Llc | Methods and compositions for modulating a genome |
| US20240417719A1 (en) | 2021-10-25 | 2024-12-19 | The Broad Institute, Inc. | Methods and compositions for editing a genome with prime editing and a recombinase |
| IL313146A (en) | 2021-12-03 | 2024-07-01 | Harvard College | Self-assembling virus-like particles for delivery of prime editors and methods of making and using same |
| WO2023205687A1 (en) | 2022-04-20 | 2023-10-26 | The Broad Institute, Inc. | Improved prime editing methods and compositions |
| WO2023240137A1 (en) | 2022-06-08 | 2023-12-14 | The Board Institute, Inc. | Evolved cas14a1 variants, compositions, and methods of making and using same in genome editing |
| WO2024077267A1 (en) | 2022-10-07 | 2024-04-11 | The Broad Institute, Inc. | Prime editing methods and compositions for treating triplet repeat disorders |
| EP4619515A1 (en) | 2022-11-17 | 2025-09-24 | The Broad Institute, Inc. | Prime editor delivery by aav |
| EP4662306A2 (en) | 2023-02-09 | 2025-12-17 | The Broad Institute Inc. | Evolved recombinases for editing a genome in combination with prime editing |
| WO2024206125A1 (en) | 2023-03-24 | 2024-10-03 | The Broad Institute, Inc. | Use of prime editing for treating sickle cell disease |
| WO2025081042A1 (en) | 2023-10-12 | 2025-04-17 | Renagade Therapeutics Management Inc. | Nickase-retron template-based precision editing system and methods of use |
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| US5322770A (en) | 1989-12-22 | 1994-06-21 | Hoffman-Laroche Inc. | Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription |
| US5643758A (en) | 1987-03-10 | 1997-07-01 | New England Biolabs, Inc. | Production and purification of a protein fused to a binding protein |
| US5654176A (en) | 1987-05-28 | 1997-08-05 | Amrad Corporation Limited | Fusion proteins containing glutathione-s-transferase |
| US5736373A (en) * | 1996-09-23 | 1998-04-07 | Becton, Dickinson And Company | Thermostable DNA polymerase from Bacillus pallidus |
| US7001745B1 (en) | 1998-09-30 | 2006-02-21 | New England Biolabs, Inc. | Intein mediated peptide ligation |
| US6436677B1 (en) * | 2000-03-02 | 2002-08-20 | Promega Corporation | Method of reverse transcription |
| US6627424B1 (en) * | 2000-05-26 | 2003-09-30 | Mj Bioworks, Inc. | Nucleic acid modifying enzymes |
| WO2003008454A2 (en) | 2001-07-18 | 2003-01-30 | Merck Patent Gmbh | Glycoprotein vi fusion proteins |
| US7670807B2 (en) * | 2004-03-10 | 2010-03-02 | East Tennessee State Univ. Research Foundation | RNA-dependent DNA polymerase from Geobacillus stearothermophilus |
| WO2006020868A2 (en) * | 2004-08-13 | 2006-02-23 | New England Biolabs, Inc. | Intracellular production of a nuclease |
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2010
- 2010-03-04 MX MX2011009205A patent/MX2011009205A/es not_active Application Discontinuation
- 2010-03-04 AU AU2010221284A patent/AU2010221284B2/en not_active Ceased
- 2010-03-04 CN CN2010800201231A patent/CN102421791A/zh active Pending
- 2010-03-04 CA CA2754476A patent/CA2754476A1/en not_active Abandoned
- 2010-03-04 US US13/254,223 patent/US10113156B2/en active Active
- 2010-03-04 BR BRPI1009221A patent/BRPI1009221A2/pt not_active IP Right Cessation
- 2010-03-04 EP EP10749312.4A patent/EP2403941B1/en active Active
- 2010-03-04 WO PCT/US2010/026165 patent/WO2010102085A2/en not_active Ceased
- 2010-03-04 EP EP18184036.4A patent/EP3415621A1/en not_active Withdrawn
- 2010-03-04 JP JP2011553101A patent/JP5882064B2/ja active Active
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2011
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- 2011-10-03 ZA ZA2011/07207A patent/ZA201107207B/en unknown
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2015
- 2015-08-03 JP JP2015153175A patent/JP2015193666A/ja not_active Withdrawn
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2016
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2018
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- 2018-08-01 US US16/051,544 patent/US20180340158A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US10150955B2 (en) | 2018-12-11 |
| JP2012519489A (ja) | 2012-08-30 |
| JP2015193666A (ja) | 2015-11-05 |
| AU2010221284B2 (en) | 2015-10-01 |
| EP2403941A2 (en) | 2012-01-11 |
| US20180340158A1 (en) | 2018-11-29 |
| EP2403941B1 (en) | 2018-10-03 |
| WO2010102085A4 (en) | 2011-01-06 |
| IL214913A0 (en) | 2011-11-30 |
| MX2011009205A (es) | 2011-09-30 |
| AU2010221284A1 (en) | 2011-10-27 |
| ZA201107207B (en) | 2012-12-27 |
| WO2010102085A3 (en) | 2010-11-18 |
| WO2010102085A2 (en) | 2010-09-10 |
| US10113156B2 (en) | 2018-10-30 |
| US20180346890A1 (en) | 2018-12-06 |
| JP5882064B2 (ja) | 2016-03-09 |
| CN102421791A (zh) | 2012-04-18 |
| US10858636B2 (en) | 2020-12-08 |
| EP3415621A1 (en) | 2018-12-19 |
| IL214913A (en) | 2017-06-29 |
| EP2403941A4 (en) | 2012-09-05 |
| US20120009630A1 (en) | 2012-01-12 |
| US20160289652A1 (en) | 2016-10-06 |
| BRPI1009221A2 (pt) | 2016-03-15 |
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Legal Events
| Date | Code | Title | Description |
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| EEER | Examination request |
Effective date: 20150220 |
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| FZDE | Discontinued |
Effective date: 20201216 |