AU2010221284B2 - Stabilized reverse transcriptase fusion proteins - Google Patents

Stabilized reverse transcriptase fusion proteins Download PDF

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Publication number
AU2010221284B2
AU2010221284B2 AU2010221284A AU2010221284A AU2010221284B2 AU 2010221284 B2 AU2010221284 B2 AU 2010221284B2 AU 2010221284 A AU2010221284 A AU 2010221284A AU 2010221284 A AU2010221284 A AU 2010221284A AU 2010221284 B2 AU2010221284 B2 AU 2010221284B2
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AU
Australia
Prior art keywords
reverse transcriptase
protein
seq
fusion protein
stabilized
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU2010221284A
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English (en)
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AU2010221284A1 (en
Inventor
Eman Ghanem
Alan M. Lambowitz
Georg Mohr
Sabine Mohr
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
The University of Texas System
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The University of Texas System
The University of Texas at Austin
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Publication of AU2010221284A1 publication Critical patent/AU2010221284A1/en
Application granted granted Critical
Publication of AU2010221284B2 publication Critical patent/AU2010221284B2/en
Ceased legal-status Critical Current
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/24—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a MBP (maltose binding protein)-tag

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2010221284A 2009-03-04 2010-03-04 Stabilized reverse transcriptase fusion proteins Ceased AU2010221284B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US15733209P 2009-03-04 2009-03-04
US61/157,332 2009-03-04
PCT/US2010/026165 WO2010102085A2 (en) 2009-03-04 2010-03-04 Stabilized reverse transcriptase fusion proteins

Publications (2)

Publication Number Publication Date
AU2010221284A1 AU2010221284A1 (en) 2011-10-27
AU2010221284B2 true AU2010221284B2 (en) 2015-10-01

Family

ID=42710216

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2010221284A Ceased AU2010221284B2 (en) 2009-03-04 2010-03-04 Stabilized reverse transcriptase fusion proteins

Country Status (11)

Country Link
US (4) US10113156B2 (enExample)
EP (2) EP3415621A1 (enExample)
JP (2) JP5882064B2 (enExample)
CN (1) CN102421791A (enExample)
AU (1) AU2010221284B2 (enExample)
BR (1) BRPI1009221A2 (enExample)
CA (1) CA2754476A1 (enExample)
IL (1) IL214913A (enExample)
MX (1) MX2011009205A (enExample)
WO (1) WO2010102085A2 (enExample)
ZA (1) ZA201107207B (enExample)

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WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
KR20240007715A (ko) 2016-10-14 2024-01-16 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵염기 에디터의 aav 전달
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WO2018165504A1 (en) 2017-03-09 2018-09-13 President And Fellows Of Harvard College Suppression of pain by gene editing
CN110914310A (zh) 2017-03-10 2020-03-24 哈佛大学的校长及成员们 胞嘧啶至鸟嘌呤碱基编辑器
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US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
EP3658573A1 (en) 2017-07-28 2020-06-03 President and Fellows of Harvard College Methods and compositions for evolving base editors using phage-assisted continuous evolution (pace)
EP3676376B1 (en) 2017-08-30 2025-01-15 President and Fellows of Harvard College High efficiency base editors comprising gam
WO2019070843A1 (en) * 2017-10-03 2019-04-11 Board Of Regents, The University Of Texas System INVERSE TRANSCRIPTASE WITH LTR-FREE BACKLIGHTS AND USES THEREOF
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US12406749B2 (en) 2017-12-15 2025-09-02 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
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WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
WO2020191241A1 (en) 2019-03-19 2020-09-24 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
WO2020214842A1 (en) 2019-04-17 2020-10-22 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
US20220259617A1 (en) 2019-06-13 2022-08-18 The General Hospital Corporation Engineered human-endogenous virus-like particles and methods of use thereof for delivery to cells
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
JP7839094B2 (ja) * 2020-02-17 2026-04-01 リーデクスプロ アーゲー 構造研究の促進のためのｐｒｏ－マクロボディ
BR112022017715A2 (pt) 2020-03-04 2022-11-16 Flagship Pioneering Innovations Vi Llc Métodos e composições para modular um genoma
CA3177481A1 (en) 2020-05-08 2021-11-11 David R. Liu Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence
BR112023001272A2 (pt) 2020-07-24 2023-04-04 Massachusetts Gen Hospital Partículas semelhantes a vírus aprimoradas e métodos de uso das mesmas para entrega às células
CA3193099A1 (en) 2020-09-24 2022-03-31 David R. Liu Prime editing guide rnas, compositions thereof, and methods of using the same
AU2022206476A1 (en) 2021-01-11 2023-07-20 President And Fellows Of Harvard College Prime editor variants, constructs, and methods for enhancing prime editing efficiency and precision
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US20230141630A1 (en) * 2021-08-17 2023-05-11 New England Biolabs, Inc. Rolling Circle Reverse Transcription of Circular RNA
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WO2023205687A1 (en) 2022-04-20 2023-10-26 The Broad Institute, Inc. Improved prime editing methods and compositions
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Also Published As

Publication number Publication date
IL214913A (en) 2017-06-29
WO2010102085A4 (en) 2011-01-06
CN102421791A (zh) 2012-04-18
US20120009630A1 (en) 2012-01-12
WO2010102085A2 (en) 2010-09-10
JP5882064B2 (ja) 2016-03-09
US20180346890A1 (en) 2018-12-06
US20160289652A1 (en) 2016-10-06
EP2403941B1 (en) 2018-10-03
US10113156B2 (en) 2018-10-30
CA2754476A1 (en) 2010-09-10
MX2011009205A (es) 2011-09-30
US20180340158A1 (en) 2018-11-29
EP3415621A1 (en) 2018-12-19
US10150955B2 (en) 2018-12-11
ZA201107207B (en) 2012-12-27
BRPI1009221A2 (pt) 2016-03-15
WO2010102085A3 (en) 2010-11-18
JP2015193666A (ja) 2015-11-05
EP2403941A2 (en) 2012-01-11
US10858636B2 (en) 2020-12-08
AU2010221284A1 (en) 2011-10-27
IL214913A0 (en) 2011-11-30
EP2403941A4 (en) 2012-09-05
JP2012519489A (ja) 2012-08-30

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