CN102164945B - 蛋白分离应用中的温度响应型聚合物颗粒 - Google Patents
蛋白分离应用中的温度响应型聚合物颗粒 Download PDFInfo
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- CN102164945B CN102164945B CN200980136852.0A CN200980136852A CN102164945B CN 102164945 B CN102164945 B CN 102164945B CN 200980136852 A CN200980136852 A CN 200980136852A CN 102164945 B CN102164945 B CN 102164945B
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- C—CHEMISTRY; METALLURGY
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- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L51/00—Compositions of graft polymers in which the grafted component is obtained by reactions only involving carbon-to-carbon unsaturated bonds; Compositions of derivatives of such polymers
- C08L51/02—Compositions of graft polymers in which the grafted component is obtained by reactions only involving carbon-to-carbon unsaturated bonds; Compositions of derivatives of such polymers grafted on to polysaccharides
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
- B01D15/361—Ion-exchange
- B01D15/362—Cation-exchange
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
- B01D15/3861—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography using an external stimulus
- B01D15/3876—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography using an external stimulus modifying the temperature
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
- B01J20/26—Synthetic macromolecular compounds
- B01J20/264—Synthetic macromolecular compounds derived from different types of monomers, e.g. linear or branched copolymers, block copolymers, graft copolymers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
- B01J20/26—Synthetic macromolecular compounds
- B01J20/265—Synthetic macromolecular compounds modified or post-treated polymers
- B01J20/267—Cross-linked polymers
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- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
- B01J20/282—Porous sorbents
- B01J20/285—Porous sorbents based on polymers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J39/00—Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
- B01J39/08—Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
- B01J39/16—Organic material
- B01J39/18—Macromolecular compounds
- B01J39/19—Macromolecular compounds obtained otherwise than by reactions only involving unsaturated carbon-to-carbon bonds
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- B01J39/00—Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
- B01J39/26—Cation exchangers for chromatographic processes
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F2/00—Processes of polymerisation
- C08F2/44—Polymerisation in the presence of compounding ingredients, e.g. plasticisers, dyestuffs, fillers
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F20/00—Homopolymers and copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride, ester, amide, imide or nitrile thereof
- C08F20/02—Monocarboxylic acids having less than ten carbon atoms, Derivatives thereof
- C08F20/52—Amides or imides
- C08F20/54—Amides, e.g. N,N-dimethylacrylamide or N-isopropylacrylamide
- C08F20/56—Acrylamide; Methacrylamide
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F220/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride ester, amide, imide or nitrile thereof
- C08F220/02—Monocarboxylic acids having less than ten carbon atoms; Derivatives thereof
- C08F220/52—Amides or imides
- C08F220/54—Amides, e.g. N,N-dimethylacrylamide or N-isopropylacrylamide
- C08F220/56—Acrylamide; Methacrylamide
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F226/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a single or double bond to nitrogen or by a heterocyclic ring containing nitrogen
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F26/00—Homopolymers and copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a single or double bond to nitrogen or by a heterocyclic ring containing nitrogen
- C08F26/06—Homopolymers and copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a single or double bond to nitrogen or by a heterocyclic ring containing nitrogen by a heterocyclic ring containing nitrogen
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08H—DERIVATIVES OF NATURAL MACROMOLECULAR COMPOUNDS
- C08H1/00—Macromolecular products derived from proteins
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J3/00—Processes of treating or compounding macromolecular substances
- C08J3/12—Powdering or granulating
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- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
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- C08J3/24—Crosslinking, e.g. vulcanising, of macromolecules
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L1/00—Compositions of cellulose, modified cellulose or cellulose derivatives
- C08L1/08—Cellulose derivatives
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
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- C08L29/02—Homopolymers or copolymers of unsaturated alcohols
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L51/00—Compositions of graft polymers in which the grafted component is obtained by reactions only involving carbon-to-carbon unsaturated bonds; Compositions of derivatives of such polymers
- C08L51/003—Compositions of graft polymers in which the grafted component is obtained by reactions only involving carbon-to-carbon unsaturated bonds; Compositions of derivatives of such polymers grafted on to macromolecular compounds obtained by reactions only involving unsaturated carbon-to-carbon bonds
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- C—CHEMISTRY; METALLURGY
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- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
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- C08F220/02—Monocarboxylic acids having less than ten carbon atoms; Derivatives thereof
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- C08F220/06—Acrylic acid; Methacrylic acid; Metal salts or ammonium salts thereof
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F222/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a carboxyl radical and containing at least one other carboxyl radical in the molecule; Salts, anhydrides, esters, amides, imides, or nitriles thereof
- C08F222/36—Amides or imides
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- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2333/00—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides, or nitriles thereof; Derivatives of such polymers
- C08J2333/24—Homopolymers or copolymers of amides or imides
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- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
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- C08L2555/40—Mixtures based upon bitumen or asphalt containing functional additives
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- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Analytical Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Materials Engineering (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Peptides Or Proteins (AREA)
- Processes Of Treating Macromolecular Substances (AREA)
- Medicinal Preparation (AREA)
- Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2008221604A AU2008221604B2 (en) | 2008-09-22 | 2008-09-22 | Temperature-responsive polymer particles in protein separation applications |
| AU2008221604 | 2008-09-22 | ||
| PCT/AU2009/001253 WO2010031144A1 (en) | 2008-09-22 | 2009-09-22 | Temperature-responsive polymer particles in protein separation applications |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102164945A CN102164945A (zh) | 2011-08-24 |
| CN102164945B true CN102164945B (zh) | 2015-11-25 |
Family
ID=42039031
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200980136852.0A Expired - Fee Related CN102164945B (zh) | 2008-09-22 | 2009-09-22 | 蛋白分离应用中的温度响应型聚合物颗粒 |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US8877477B2 (cg-RX-API-DMAC7.html) |
| EP (1) | EP2344517B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JP5715953B2 (cg-RX-API-DMAC7.html) |
| CN (1) | CN102164945B (cg-RX-API-DMAC7.html) |
| AU (1) | AU2008221604B2 (cg-RX-API-DMAC7.html) |
| CA (1) | CA2737404C (cg-RX-API-DMAC7.html) |
| DK (1) | DK2344517T3 (cg-RX-API-DMAC7.html) |
| ES (1) | ES2498822T3 (cg-RX-API-DMAC7.html) |
| NZ (1) | NZ591656A (cg-RX-API-DMAC7.html) |
| WO (1) | WO2010031144A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2601157A1 (en) | 2005-03-16 | 2006-09-28 | Osi Pharmaceuticals, Inc. | Biological markers predictive of anti-cancer response to epidermal growth factor receptor kinase inhibitors |
| WO2008079302A2 (en) | 2006-12-21 | 2008-07-03 | Millipore Corporation | Purification of proteins |
| US8569464B2 (en) | 2006-12-21 | 2013-10-29 | Emd Millipore Corporation | Purification of proteins |
| US20100190963A1 (en) | 2008-12-16 | 2010-07-29 | Millipore Corporation | Stirred Tank Reactor And Method |
| ES2754210T3 (es) | 2010-05-17 | 2020-04-16 | Emd Millipore Corp | Polímeros sensibles a estímulos para la purificación de biomoléculas |
| WO2012081727A1 (ja) | 2010-12-17 | 2012-06-21 | 旭化成メディカル株式会社 | 強カチオン交換基を有する温度応答性吸着剤、及びその製造方法 |
| JP6203635B2 (ja) * | 2011-08-19 | 2017-09-27 | 国立大学法人九州大学 | イオン濃度勾配発生システム、装置、方法、及び、温度応答性電解質材料 |
| SG10201701224UA (en) * | 2012-03-12 | 2017-04-27 | Merck Patent Gmbh | Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode |
| EP2961762B1 (en) * | 2013-02-26 | 2022-04-06 | Merck Millipore Ltd. | Mixed-mode chromatography membranes |
| JP2014161833A (ja) * | 2013-02-27 | 2014-09-08 | Mitsubishi Chemicals Corp | タンパク質用アフィニティ分離剤 |
| US10400007B2 (en) | 2013-04-16 | 2019-09-03 | Asahi Kasei Medical Co., Ltd. | Method for purifying antibody protein |
| CN105980047B (zh) | 2013-12-12 | 2019-01-04 | Emd密理博公司 | 使用含丙烯酰胺的过滤器分离蛋白 |
| US11092574B2 (en) * | 2013-12-24 | 2021-08-17 | Waters Technologies Corporation | Materials for hydrophilic interaction chromatography and processes for preparation and use thereof for analysis of glycoproteins and glycopeptides |
| US10119944B2 (en) | 2013-12-24 | 2018-11-06 | Waters Technologies Corporation | Materials for hydrophilic interaction chromatography and processes for preparation and use thereof for analysis of glycoproteins and glycopeptides |
| CN103965375A (zh) * | 2014-05-07 | 2014-08-06 | 集美大学 | 一种琼脂糖改性衍生产品的制备方法 |
| US10064244B2 (en) | 2015-09-10 | 2018-08-28 | Brava Home, Inc. | Variable peak wavelength cooking instrument with support tray |
| JP6836260B2 (ja) * | 2016-08-23 | 2021-02-24 | 国立大学法人信州大学 | 高分子粒子およびその製造方法 |
| US11944941B2 (en) | 2018-04-30 | 2024-04-02 | Trustees Of Tufts College | Chemoselective nanoporous membranes |
| AU2019391307B2 (en) * | 2018-12-03 | 2025-10-30 | Centre National De La Recherche Scientifique | Amphipols |
| JP2020157190A (ja) * | 2019-03-25 | 2020-10-01 | 株式会社日立ハイテクサイエンス | 生体成分分離用高分子ファイバー、生体成分分離用カラム、計測装置及び生体成分分離装置 |
| JP7228236B2 (ja) * | 2019-03-25 | 2023-02-24 | 株式会社日立ハイテクサイエンス | 遠心分離カラム及び分析対象物質の分析方法 |
| KR102245836B1 (ko) * | 2019-10-28 | 2021-04-28 | 광주과학기술원 | 온도 자극반응성 공중합체, 이를 포함하는 나노섬유 구조체 및 나노섬유 구조체 제조방법 |
| CN113648984A (zh) * | 2021-09-24 | 2021-11-16 | 长春工业大学 | 一种温度-pH响应分子印迹纤维膜及其制备方法 |
| WO2025177962A1 (ja) * | 2024-02-22 | 2025-08-28 | 三菱ケミカル株式会社 | イオン交換樹脂の製造方法、化合物の分離方法、及び陽イオン交換樹脂 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999057134A1 (en) * | 1998-05-06 | 1999-11-11 | Genentech, Inc. | Protein purification by ion exchange chromatography |
| CN1658902A (zh) * | 2002-06-01 | 2005-08-24 | 麦格纳麦迪克斯有限公司 | 用于生化分析、诊断和治疗的具有可变物理结构的热敏聚合物载体 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE437270B (sv) * | 1983-07-19 | 1985-02-18 | Pharmacia Ab | Separationsmaterial av tverbunden agaros och dess framstellning separationsmaterial av tverbunden agaros och dess framstellning |
| US6641735B1 (en) * | 2000-03-23 | 2003-11-04 | Japan Chemical Innovation Institute | Separatory material with the use of stimulus-responsive polymer and separation method by using the separatory material |
| WO2001074482A1 (en) * | 2000-04-05 | 2001-10-11 | Japan Chemical Innovation Institute | Novel material for use in separation and separating method using the same |
-
2008
- 2008-09-22 AU AU2008221604A patent/AU2008221604B2/en not_active Ceased
-
2009
- 2009-09-22 EP EP09813911.6A patent/EP2344517B1/en not_active Not-in-force
- 2009-09-22 WO PCT/AU2009/001253 patent/WO2010031144A1/en not_active Ceased
- 2009-09-22 NZ NZ591656A patent/NZ591656A/xx not_active IP Right Cessation
- 2009-09-22 DK DK09813911.6T patent/DK2344517T3/da active
- 2009-09-22 US US13/062,960 patent/US8877477B2/en not_active Expired - Fee Related
- 2009-09-22 JP JP2011527158A patent/JP5715953B2/ja not_active Expired - Fee Related
- 2009-09-22 CN CN200980136852.0A patent/CN102164945B/zh not_active Expired - Fee Related
- 2009-09-22 ES ES09813911.6T patent/ES2498822T3/es active Active
- 2009-09-22 CA CA2737404A patent/CA2737404C/en not_active Expired - Fee Related
-
2014
- 2014-09-29 US US14/499,653 patent/US9944784B2/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999057134A1 (en) * | 1998-05-06 | 1999-11-11 | Genentech, Inc. | Protein purification by ion exchange chromatography |
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Also Published As
| Publication number | Publication date |
|---|---|
| JP5715953B2 (ja) | 2015-05-13 |
| EP2344517A4 (en) | 2013-03-27 |
| AU2008221604A1 (en) | 2010-04-08 |
| NZ591656A (en) | 2012-11-30 |
| ES2498822T3 (es) | 2014-09-25 |
| EP2344517A1 (en) | 2011-07-20 |
| EP2344517B1 (en) | 2014-06-04 |
| CN102164945A (zh) | 2011-08-24 |
| WO2010031144A1 (en) | 2010-03-25 |
| DK2344517T3 (da) | 2014-09-01 |
| US20120052550A1 (en) | 2012-03-01 |
| AU2008221604B2 (en) | 2010-04-22 |
| CA2737404C (en) | 2018-04-10 |
| US8877477B2 (en) | 2014-11-04 |
| US20150018440A1 (en) | 2015-01-15 |
| US9944784B2 (en) | 2018-04-17 |
| CA2737404A1 (en) | 2010-03-25 |
| JP2012502924A (ja) | 2012-02-02 |
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