CN102164945B - 蛋白分离应用中的温度响应型聚合物颗粒 - Google Patents

蛋白分离应用中的温度响应型聚合物颗粒 Download PDF

Info

Publication number
CN102164945B
CN102164945B CN200980136852.0A CN200980136852A CN102164945B CN 102164945 B CN102164945 B CN 102164945B CN 200980136852 A CN200980136852 A CN 200980136852A CN 102164945 B CN102164945 B CN 102164945B
Authority
CN
China
Prior art keywords
unit
temperature
units
solution
linked
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN200980136852.0A
Other languages
English (en)
Chinese (zh)
Other versions
CN102164945A (zh
Inventor
B·W·伍恩顿
M·T·W·赫恩
P·马哈詹
K·德西尔瓦
W·R·杰克森
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Commonwealth Scientific and Industrial Research Organisation
Monash University
Original Assignee
Commonwealth Scientific and Industrial Research Organisation
Monash University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Commonwealth Scientific and Industrial Research Organisation, Monash University filed Critical Commonwealth Scientific and Industrial Research Organisation
Publication of CN102164945A publication Critical patent/CN102164945A/zh
Application granted granted Critical
Publication of CN102164945B publication Critical patent/CN102164945B/zh
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L51/00—Compositions of graft polymers in which the grafted component is obtained by reactions only involving carbon-to-carbon unsaturated bonds; Compositions of derivatives of such polymers
    • C08L51/02—Compositions of graft polymers in which the grafted component is obtained by reactions only involving carbon-to-carbon unsaturated bonds; Compositions of derivatives of such polymers grafted on to polysaccharides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3861—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography using an external stimulus
    • B01D15/3876—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography using an external stimulus modifying the temperature
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
    • B01J20/26—Synthetic macromolecular compounds
    • B01J20/264—Synthetic macromolecular compounds derived from different types of monomers, e.g. linear or branched copolymers, block copolymers, graft copolymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
    • B01J20/26—Synthetic macromolecular compounds
    • B01J20/265—Synthetic macromolecular compounds modified or post-treated polymers
    • B01J20/267—Cross-linked polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/282—Porous sorbents
    • B01J20/285—Porous sorbents based on polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J39/00—Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
    • B01J39/08—Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
    • B01J39/16—Organic material
    • B01J39/18—Macromolecular compounds
    • B01J39/19—Macromolecular compounds obtained otherwise than by reactions only involving unsaturated carbon-to-carbon bonds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J39/00—Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
    • B01J39/26—Cation exchangers for chromatographic processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F2/00—Processes of polymerisation
    • C08F2/44—Polymerisation in the presence of compounding ingredients, e.g. plasticisers, dyestuffs, fillers
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F20/00—Homopolymers and copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride, ester, amide, imide or nitrile thereof
    • C08F20/02—Monocarboxylic acids having less than ten carbon atoms, Derivatives thereof
    • C08F20/52—Amides or imides
    • C08F20/54—Amides, e.g. N,N-dimethylacrylamide or N-isopropylacrylamide
    • C08F20/56—Acrylamide; Methacrylamide
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F220/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride ester, amide, imide or nitrile thereof
    • C08F220/02—Monocarboxylic acids having less than ten carbon atoms; Derivatives thereof
    • C08F220/52—Amides or imides
    • C08F220/54—Amides, e.g. N,N-dimethylacrylamide or N-isopropylacrylamide
    • C08F220/56—Acrylamide; Methacrylamide
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F226/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a single or double bond to nitrogen or by a heterocyclic ring containing nitrogen
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F26/00—Homopolymers and copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a single or double bond to nitrogen or by a heterocyclic ring containing nitrogen
    • C08F26/06—Homopolymers and copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a single or double bond to nitrogen or by a heterocyclic ring containing nitrogen by a heterocyclic ring containing nitrogen
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08H—DERIVATIVES OF NATURAL MACROMOLECULAR COMPOUNDS
    • C08H1/00—Macromolecular products derived from proteins
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J3/00—Processes of treating or compounding macromolecular substances
    • C08J3/12—Powdering or granulating
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J3/00—Processes of treating or compounding macromolecular substances
    • C08J3/24—Crosslinking, e.g. vulcanising, of macromolecules
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L1/00—Compositions of cellulose, modified cellulose or cellulose derivatives
    • C08L1/08—Cellulose derivatives
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L29/00—Compositions of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by an alcohol, ether, aldehydo, ketonic, acetal or ketal radical; Compositions of hydrolysed polymers of esters of unsaturated alcohols with saturated carboxylic acids; Compositions of derivatives of such polymers
    • C08L29/02—Homopolymers or copolymers of unsaturated alcohols
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L5/00—Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L51/00—Compositions of graft polymers in which the grafted component is obtained by reactions only involving carbon-to-carbon unsaturated bonds; Compositions of derivatives of such polymers
    • C08L51/003—Compositions of graft polymers in which the grafted component is obtained by reactions only involving carbon-to-carbon unsaturated bonds; Compositions of derivatives of such polymers grafted on to macromolecular compounds obtained by reactions only involving unsaturated carbon-to-carbon bonds
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F220/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride ester, amide, imide or nitrile thereof
    • C08F220/02—Monocarboxylic acids having less than ten carbon atoms; Derivatives thereof
    • C08F220/04—Acids; Metal salts or ammonium salts thereof
    • C08F220/06—Acrylic acid; Methacrylic acid; Metal salts or ammonium salts thereof
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F222/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a carboxyl radical and containing at least one other carboxyl radical in the molecule; Salts, anhydrides, esters, amides, imides, or nitriles thereof
    • C08F222/36—Amides or imides
    • C08F222/38—Amides
    • C08F222/385—Monomers containing two or more (meth)acrylamide groups, e.g. N,N'-methylenebisacrylamide
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2333/00—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides, or nitriles thereof; Derivatives of such polymers
    • C08J2333/24—Homopolymers or copolymers of amides or imides
    • C08J2333/26—Homopolymers or copolymers of acrylamide or methacrylamide
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L2555/00—Characteristics of bituminous mixtures
    • C08L2555/40—Mixtures based upon bitumen or asphalt containing functional additives
    • C08L2555/80—Macromolecular constituents

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Analytical Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Materials Engineering (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Peptides Or Proteins (AREA)
  • Processes Of Treating Macromolecular Substances (AREA)
  • Medicinal Preparation (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
CN200980136852.0A 2008-09-22 2009-09-22 蛋白分离应用中的温度响应型聚合物颗粒 Expired - Fee Related CN102164945B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AU2008221604A AU2008221604B2 (en) 2008-09-22 2008-09-22 Temperature-responsive polymer particles in protein separation applications
AU2008221604 2008-09-22
PCT/AU2009/001253 WO2010031144A1 (en) 2008-09-22 2009-09-22 Temperature-responsive polymer particles in protein separation applications

Publications (2)

Publication Number Publication Date
CN102164945A CN102164945A (zh) 2011-08-24
CN102164945B true CN102164945B (zh) 2015-11-25

Family

ID=42039031

Family Applications (1)

Application Number Title Priority Date Filing Date
CN200980136852.0A Expired - Fee Related CN102164945B (zh) 2008-09-22 2009-09-22 蛋白分离应用中的温度响应型聚合物颗粒

Country Status (10)

Country Link
US (2) US8877477B2 (cg-RX-API-DMAC10.html)
EP (1) EP2344517B1 (cg-RX-API-DMAC10.html)
JP (1) JP5715953B2 (cg-RX-API-DMAC10.html)
CN (1) CN102164945B (cg-RX-API-DMAC10.html)
AU (1) AU2008221604B2 (cg-RX-API-DMAC10.html)
CA (1) CA2737404C (cg-RX-API-DMAC10.html)
DK (1) DK2344517T3 (cg-RX-API-DMAC10.html)
ES (1) ES2498822T3 (cg-RX-API-DMAC10.html)
NZ (1) NZ591656A (cg-RX-API-DMAC10.html)
WO (1) WO2010031144A1 (cg-RX-API-DMAC10.html)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006101925A2 (en) 2005-03-16 2006-09-28 Osi Pharmaceuticals, Inc. Biological markers predictive of anti-cancer response to epidermal growth factor receptor kinase inhibitors
US8163886B2 (en) 2006-12-21 2012-04-24 Emd Millipore Corporation Purification of proteins
US8569464B2 (en) 2006-12-21 2013-10-29 Emd Millipore Corporation Purification of proteins
DK2370561T3 (da) 2008-12-16 2019-10-21 Emd Millipore Corp Omrøringstankreaktor og fremgangsmåde
CN102892791B (zh) 2010-05-17 2017-05-17 Emd密理博公司 用于纯化生物分子的刺激响应性聚合物
JP5981133B2 (ja) * 2010-12-17 2016-08-31 旭化成メディカル株式会社 強カチオン交換基を有する温度応答性吸着剤、及びその製造方法
EP3795242A1 (en) * 2011-08-19 2021-03-24 Kyushu University, National University Corporation Membrane for recovering co2
SG10201701224UA (en) * 2012-03-12 2017-04-27 Merck Patent Gmbh Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode
JP6639236B2 (ja) * 2013-02-26 2020-02-05 ナトリックス セパレイションズ インコーポレーテッド 混合モードクロマトグラフィー膜
JP2014161833A (ja) * 2013-02-27 2014-09-08 Mitsubishi Chemicals Corp タンパク質用アフィニティ分離剤
JP6163541B2 (ja) * 2013-04-16 2017-07-12 旭化成メディカル株式会社 抗体タンパク質の精製方法
US9951101B2 (en) 2013-12-12 2018-04-24 Emd Millipore Corporation Protein separations using an acrylamide containing filter
US11092574B2 (en) 2013-12-24 2021-08-17 Waters Technologies Corporation Materials for hydrophilic interaction chromatography and processes for preparation and use thereof for analysis of glycoproteins and glycopeptides
US10119944B2 (en) 2013-12-24 2018-11-06 Waters Technologies Corporation Materials for hydrophilic interaction chromatography and processes for preparation and use thereof for analysis of glycoproteins and glycopeptides
CN103965375A (zh) * 2014-05-07 2014-08-06 集美大学 一种琼脂糖改性衍生产品的制备方法
US10064244B2 (en) 2015-09-10 2018-08-28 Brava Home, Inc. Variable peak wavelength cooking instrument with support tray
JP6836260B2 (ja) * 2016-08-23 2021-02-24 国立大学法人信州大学 高分子粒子およびその製造方法
WO2019212889A1 (en) * 2018-04-30 2019-11-07 Trustees Of Tufts College Chemoselective nanoporous membranes
EP3891197A2 (fr) * 2018-12-03 2021-10-13 Centre national de la recherche scientifique Amphipols
JP7228236B2 (ja) * 2019-03-25 2023-02-24 株式会社日立ハイテクサイエンス 遠心分離カラム及び分析対象物質の分析方法
JP2020157190A (ja) * 2019-03-25 2020-10-01 株式会社日立ハイテクサイエンス 生体成分分離用高分子ファイバー、生体成分分離用カラム、計測装置及び生体成分分離装置
KR102245836B1 (ko) * 2019-10-28 2021-04-28 광주과학기술원 온도 자극반응성 공중합체, 이를 포함하는 나노섬유 구조체 및 나노섬유 구조체 제조방법
CN113648984A (zh) * 2021-09-24 2021-11-16 长春工业大学 一种温度-pH响应分子印迹纤维膜及其制备方法
CN116891551A (zh) * 2023-08-08 2023-10-17 北京理工大学 一种星型聚合物及其制备方法和应用
WO2025177962A1 (ja) * 2024-02-22 2025-08-28 三菱ケミカル株式会社 イオン交換樹脂の製造方法、化合物の分離方法、及び陽イオン交換樹脂

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999057134A1 (en) * 1998-05-06 1999-11-11 Genentech, Inc. Protein purification by ion exchange chromatography
CN1658902A (zh) * 2002-06-01 2005-08-24 麦格纳麦迪克斯有限公司 用于生化分析、诊断和治疗的具有可变物理结构的热敏聚合物载体

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE437270B (sv) * 1983-07-19 1985-02-18 Pharmacia Ab Separationsmaterial av tverbunden agaros och dess framstellning separationsmaterial av tverbunden agaros och dess framstellning
US6641735B1 (en) * 2000-03-23 2003-11-04 Japan Chemical Innovation Institute Separatory material with the use of stimulus-responsive polymer and separation method by using the separatory material
AU2001246843A1 (en) * 2000-04-05 2001-10-15 Japan Chemical Innovation Institute Novel material for use in separation and separating method using the same

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999057134A1 (en) * 1998-05-06 1999-11-11 Genentech, Inc. Protein purification by ion exchange chromatography
CN1658902A (zh) * 2002-06-01 2005-08-24 麦格纳麦迪克斯有限公司 用于生化分析、诊断和治疗的具有可变物理结构的热敏聚合物载体

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
Modified release of hydrophilic, hydrophobic and peptide agents from ionized amphiphilic gel networks;Hua Yu等;《Journal of Controlled Release》;19950531;第34卷(第2期);第117-127页 *
Novel chromatographic separation — The potential of smart polymers;Pankaj Maharjan等;《Innovative Food Science and Emerging Technologies》;20080430;第9卷(第2期);第232-242页,尤其是第238页5.2、图9 *
N-异丙基丙烯酰胺共聚物的温度敏感性研究;陈瑜等;《胶体与聚合物》;20010930;第19卷(第3期);第5-7页 *
Preparation and characterization of N-isopropylacrylamide/acrylic acid copolymer core–shell microgel particles;Aslam Khan;《Journal of Colloid and Interface Science》;20070516;第313卷(第2期);第697-704页 *
Preparation and characterization of pH- and temperature-sensitive pullulan microspheres for controlled release of drugs;Gheorghe Fundueanu等;《Biomaterials》;20080408;第29卷(第18期);第2767-2775页 *
Preparation and Swelling Properties of Poly(NIPAM"Minigel"Particles Prepared by Inverse Suspension Polymerization;Peter John Dowding等;《Journal of Colloid and Interface Science》;20000115;第221卷(第2期);第268-272页 *
Preparation of thermoresponsive adsorption chromatography matrices and application to separation of blood proteins with them;Nagase et al;《Proceedings of symposium on macromolecules》;20080909;第57卷(第2期);全文 *

Also Published As

Publication number Publication date
EP2344517A4 (en) 2013-03-27
NZ591656A (en) 2012-11-30
ES2498822T3 (es) 2014-09-25
US8877477B2 (en) 2014-11-04
US20120052550A1 (en) 2012-03-01
US20150018440A1 (en) 2015-01-15
CN102164945A (zh) 2011-08-24
JP5715953B2 (ja) 2015-05-13
AU2008221604B2 (en) 2010-04-22
DK2344517T3 (da) 2014-09-01
AU2008221604A1 (en) 2010-04-08
US9944784B2 (en) 2018-04-17
EP2344517A1 (en) 2011-07-20
CA2737404A1 (en) 2010-03-25
CA2737404C (en) 2018-04-10
JP2012502924A (ja) 2012-02-02
EP2344517B1 (en) 2014-06-04
WO2010031144A1 (en) 2010-03-25

Similar Documents

Publication Publication Date Title
US9944784B2 (en) Temperature-responsive polymer particles in protein separation applications
CN104245078B (zh) 分离方法和分离基质
CN1946479A (zh) 一种制备分离基质的方法
JP5474881B2 (ja) 向上したクロマトグラフィー媒体の製造方法および使用方法
Nagase et al. Mixed polymer brush as a functional ligand of silica beads for temperature-modulated hydrophobic and electrostatic interactions
US20130225701A1 (en) Grafting method to improve chromatography media performance
JP5981133B2 (ja) 強カチオン交換基を有する温度応答性吸着剤、及びその製造方法
JP6063529B2 (ja) 物質分離用前処理カートリッジ及びそれを利用した前処理方法
WO2001074482A1 (en) Novel material for use in separation and separating method using the same
US7119145B2 (en) Surface graft modified resins and formation thereof
WO2009043191A2 (en) Method for producing macro-porous materials
JP5631271B2 (ja) 改良されたクロマトグラフィー媒体の製造方法および使用方法
Tan et al. Batch binding studies with thermo-responsive polymer grafted sepharose 6 fast flow sorbents under different temperature and protein loading conditions
JP5635725B2 (ja) 生理活性高分子物質の分離方法
US20030162853A1 (en) Use of adsorbent polymer particles in DNA separation
JP2003524680A (ja) 刺激応答性高分子を用いた親和力制御型材料および該材料を用いた分離精製方法
US7015281B2 (en) Alkali-stable hydrophilic sorbents for ion-exchange chromatography
CN119060256B (zh) 一种耐盐型强阳离子交换层析介质的制备方法
JPH05133947A (ja) 液体クロマトグラフイー用担体およびそれを用いた液体クロマトグラフイー法
Masini Smart Porous Monoliths for Chromatographic Separations
JP2004339383A (ja) 機能性ピペットチップ及びチューブ

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20151125

Termination date: 20180922