CN101999307A - Natural reproduction method for sowing dendrobium on boards and barks - Google Patents
Natural reproduction method for sowing dendrobium on boards and barks Download PDFInfo
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- CN101999307A CN101999307A CN2010105143535A CN201010514353A CN101999307A CN 101999307 A CN101999307 A CN 101999307A CN 2010105143535 A CN2010105143535 A CN 2010105143535A CN 201010514353 A CN201010514353 A CN 201010514353A CN 101999307 A CN101999307 A CN 101999307A
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Abstract
The invention discloses a natural reproduction method for sowing dendrobium on boards and barks. The method comprises the following steps of: 1, mixing seeds into water to be made into seed liquid; 2, spraying the seed liquid on a plant material plate seedbed constructed in advance, wherein the roughness Rz of the seedbed is at least 0.06mm; and 3, managing water and fertilizer in later stage. The seedbed made of barks, boards or fiberboards and the method of spray seeding are adopted for seeding, and therefore the defects that the rooting difficulty is increased as dendrobium seed embryo is small, undergrown and ablastous, and dendrobium seeds are easily washed away by water are solved. Dendrobium seedlings grow uniformly, and the operation is simple, thus the method can be suitable for large-scale dendrobium industrialization planting.
Description
Technical field
The present invention relates to a kind of stem of noble dendrobium seedling breeding method, relate to a kind of natural propagation method of stem of noble dendrobium plank bark sowing in particular.
Technical background
The eighties in 20th century, the how tame scientific research institution of China begins the stem of noble dendrobium is carried out quick reproduction technique and planting technology research, and quick reproduction technique mainly concentrates in the research of tissue culture technique.At present, the transplanting survival rate of tissue cultivating seedling has had and has increased substantially, and stem of noble dendrobium quick reproduction technique mode is based on tissue-culturing rapid propagation, and plant division, cuttage and the breeding of high bud are auxilliary.But, tissue culture technology cost height, have high input, demand strict technology, seedling costs an arm and a leg, peasant household is difficult to accept, explores one simple to operate, transplanting survival rate is high, cultivate the low quick reproduction technique approach of cost, is the key of promotion stem of noble dendrobium industry fast development.
Stem of noble dendrobium seed is tiny, be Powdered, and each capsule contains and tens thousand ofly directly utilizes seed the outdoor by artificial seeding of carrying out to hundreds thousand of grains, realizes that seed natural propagation is that the scientific worker who is engaged in the stem of noble dendrobium and even orchid research dreams of.At present, stem of noble dendrobium tissue-culturing rapid propagation has been realized batch production production, and its breeding approach is still based on the embryo aseptic seeding, and how making the embryo sowing is the key of technical research from vitro developing outside test tube.The outdoor seeding technique research of relevant orchid embryo started from for 20 beginnings of the century.Bernard is in the relation of reported first fungi in 1903 and orchid kind embryo germination, he infected embryo with the mycorhiza of purifying afterwards, it is good to plant embryo germination, and formation seedling, confirmed that fungi promotes the effect of kind of embryo germination, and think and have only the orchid embryo that exists under field conditions (factors) by suitable fungal infection and can successfully sprout.Now the symbiotrophy relation of orchid and mycorrhizal fungi, selectivity etc. have been carried out deep research, and thought that the orchid family originated from fungus is extensive, mainly be present in soil, dry branches and fallen leaves and biological residual body, also may be present in attached Your Majesty or the air.At present, the symbiotic germination technology is applied at partly living Lan Shangyi such as rhizoma Gastrodiaes, but does not make a breakthrough yet on epiphytic orchids such as the stem of noble dendrobium.
Summary of the invention
For addressing the above problem, the invention provides a kind of natural propagation method of stem of noble dendrobium plank bark sowing, this method may further comprise the steps:
1) seed is sneaked into makes seed liquor in the water;
2) seed liquor is sprayed at the roughness R that builds in advance
zOn the dull and stereotyped seedbed of the vegetable material of ≧ 0.06mm;
3) later stage water and fertilizer management.
The described preparation seed liquor of step 1) is: the seed in the stem of noble dendrobium mellow fruit is sneaked into 2000~4000ml contain in the aqueous solution of 30~35% thin rice gruel, placed 2~3 hours, stir before using.
In step 2) described in the sprinkling seed liquor before, also on dull and stereotyped seedbed, sprayed 5~10% cow dungs or pig manure suspension.
Step 2) the roughness R that builds described in
zThe dull and stereotyped seedbed of the vegetable material of ≧ 0.06mm is: terrain clearance 50~100cm, wide 50~150cm, long 3~10m, dull and stereotyped seedbed horizontal tilt degree ﹤ 30 degree.Dull and stereotyped seedbed matsurface upwards.Top, dull and stereotyped seedbed is provided with the shed of 50~70cm, and the shed top is provided with jet pipe and shower nozzle.
The material in the dull and stereotyped seedbed of described vegetable material is: a kind of in plank, bark or the fiber board.
The described final-period management of step 3) is: then 2~March per 15 days atomizing water sprays 1 time to the May 1 after planting; After May 1, every day in dry season, the atomizing water spray was 1~2 time, and per 3 days of rainy season, the atomizing water spray was 1 time; Enrich nutrient solution after July 1, the atomizing water spray was 1 time in per 7 days, stopped to use nutrient solution after October 1.
Useful technique effect of the present invention is: the seedbed of adopting bark, plank or fiber board to make, adopt the method sowing of spraying sowing, solved that stem of noble dendrobium seed embryo is tiny, hypoplasia and do not have the plumule difficulty of taking root and strengthen, and easy washed away shortcoming, stem of noble dendrobium seedling early growth is even, simple to operate, applicable to large-scale stem of noble dendrobium industrialization plantation.
Embodiment
The preparation in embodiment 1 dull and stereotyped seedbed
Preparation plank seedbed is sawn into the long plank of width greater than 10cm with timber, allows the plank surface slightly release souls from purgatory R with sawtooth gap bigger band saw or wheel saw as far as possible when saw system plank
z≧ 0.06mm if the timber that is adopted is wooden fine and smooth, then needs to make the plank surface slightly release souls from purgatory R plank table hacking second
z≧ 0.06mm; The plank for preparing is made the plank seedbed with rope or wooden peg, can not produce the slit between each plank, can be for the length user in seedbed according to the selection voluntarily in above-mentioned length and width scope of place needs.
Preparation bark seedbed selects area greater than 100cm
2Shape is the bark of rule, and there is width on the bark surface greater than the inner face of the selection bark of the crackle of the 1mm surface as the seedbed; Adopt bark inner face R
zDuring ﹤ 0.03mm, answer hacking bark inner face; With bark below add the lead-in nail and make the flat board that does not have the slit between each sheet bark, the flat board for preparing is made the bark seedbed with rope or wooden peg, can not produce the slit between each plank, can be for the length user in seedbed according to the selection voluntarily in above-mentioned length and width scope of place needs.
Prepare the fiber board seedbed, vegetable fiber is suppressed into slightly releases souls from purgatory R
zThe flat board of ≧ 0.06mm can become the fiber board seedbed.
All seedbed terrain clearance 50~100cm, wide 50~150cm, long 3~10m, dull and stereotyped seedbed horizontal tilt degree ﹤ 30 degree.Dull and stereotyped seedbed matsurface upwards.Top, dull and stereotyped seedbed is provided with the shed of 50~70cm, and the shed top is provided with jet pipe and shower nozzle.
The cultivation of embodiment 2 stems of noble dendrobium
The seed of stem of noble dendrobium fruit of choosing completely filled fruit, 100% maturation, natural cracking is as stem of noble dendrobium seed.
Get 3000ml and sneak into dry matter, 1 stem of noble dendrobium fruit of choosing is cut open, all stem of noble dendrobium seeds in the fruit are added in big flood, left standstill 2~3 hours, stir before using stem of noble dendrobium seed is fully suspended, obtain seed liquor greater than 5% 30~35% thin rice gruel aqueous solution.
The suspension of 5~10% cow dungs or pig manure evenly is sprayed at surface, embodiment 1 described seedbed with sprayer, and the fiber to cow dung or the pig manure evenly covers till the seedbed; When treating that the surface, seedbed does not have open fire, seed liquor is evenly sprayed on the surface, seedbed; The consumption of seed sprays 100~200m for the seed of each stem of noble dendrobium fruit
2The seedbed area.
Water of spray in per 15 days to the May 1 then after planting; After May 1, spray 1~2 fine every day, water of per 3 days of rainy day spray; Enrich nutrient solution after July 1, spray in per 7 days 1 time stops to use nutrient solution after October 1;
Wherein, nutrient solution is that the nutrient solution of desaccharification prescription of full-valence nutrition liquor, 1/2MS or the MS of soilless culture sprays, and the spraying method of nutrient solution and water is the atomizing mode.
Claims (6)
1. the natural propagation method of stem of noble dendrobium plank bark sowing, it is characterized in that: this method may further comprise the steps:
1) seed is sneaked into makes seed liquor in the water;
2) seed liquor is sprayed at the roughness R that builds in advance
zOn the dull and stereotyped seedbed of the vegetable material of ≧ 0.06mm;
3) later stage water and fertilizer management.
2. the natural propagation method of stem of noble dendrobium plank bark sowing according to claim 1, it is characterized in that: the described preparation seed liquor of step 1) is: the seed in the stem of noble dendrobium mellow fruit is sneaked into 2000~4000ml contain in the aqueous solution of 30~35% thin rice gruel, placed 2~3 hours, and stirred before using.
3. the natural propagation method of stem of noble dendrobium plank bark according to claim 1 sowing is characterized in that: in step 2) described in the sprinkling seed liquor before, also on dull and stereotyped seedbed, sprayed 5~10% cow dungs or pig manure suspension.
4. the natural propagation method of stem of noble dendrobium plank bark according to claim 1 sowing is characterized in that: step 2) described in the roughness R that builds
zThe dull and stereotyped seedbed of the vegetable material of ≧ 0.06mm is: terrain clearance 50~100cm, wide 50~150cm, long 3~10m, dull and stereotyped seedbed horizontal tilt degree ﹤ 30 degree; Dull and stereotyped seedbed matsurface upwards; Top, dull and stereotyped seedbed is provided with the shed of 50~70cm, and the shed top is provided with jet pipe and shower nozzle.
5. according to the natural propagation method of claim 1, the sowing of 3 or 4 described stem of noble dendrobium plank barks, it is characterized in that: the material in the dull and stereotyped seedbed of described vegetable material is: a kind of in plank, bark or the fiber board.
6. the natural propagation method of stem of noble dendrobium plank bark according to claim 1 sowing, it is characterized in that: the described final-period management of step 3) is: then 2~March per 15 days atomizing water sprays 1 time to the May 1 after planting; After May 1, every day in dry season, the atomizing water spray was 1~2 time, and per 3 days of rainy season, the atomizing water spray was 1 time; Enrich nutrient solution after July 1, the atomizing water spray was 1 time in per 7 days, stopped to use nutrient solution after October 1.
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CN2010105143535A CN101999307B (en) | 2010-10-21 | 2010-10-21 | Natural reproduction method for sowing dendrobium |
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CN2010105143535A CN101999307B (en) | 2010-10-21 | 2010-10-21 | Natural reproduction method for sowing dendrobium |
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Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102440130A (en) * | 2011-09-22 | 2012-05-09 | 澄思源生物科技(上海)有限公司 | Method for natural propagation of dendrobium officinale seeds |
CN103004445A (en) * | 2013-01-06 | 2013-04-03 | 戴亚峰 | Method for directly seedling dendrobe seedlings by using plastic greenhouse |
CN103168667A (en) * | 2011-12-23 | 2013-06-26 | 何寒 | Rapid propagation technology of Dendrobium officinale |
CN103931410A (en) * | 2014-05-06 | 2014-07-23 | 龙陵县云河石斛开发有限责任公司 | Open forest seedling cultivation method for Den. devonianum Paxt |
CN106577232A (en) * | 2016-12-13 | 2017-04-26 | 重庆市中药研究院 | Natural propagation method for sowing seeds of dendrobium flexicaule on palisades |
CN108782199A (en) * | 2018-05-19 | 2018-11-13 | 安徽省霍山县霍鼎仙石斛开发有限公司 | A kind of implantation methods of growing nonparasitically upon another plant of the stem of noble dendrobium |
CN111616038A (en) * | 2020-05-20 | 2020-09-04 | 龙陵县石斛研究所 | Dendrobium devonianum seedling growing method |
CN112021168A (en) * | 2020-09-25 | 2020-12-04 | 广东永生源生物科技有限公司 | Method for planting dendrobium officinale by using straw ropes to simulate wild trees |
CN115005073A (en) * | 2022-06-22 | 2022-09-06 | 中国热带农业科学院热带作物品种资源研究所 | Dendrobium autumn open seeding method based on napkin |
Citations (3)
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CN101336605A (en) * | 2007-07-07 | 2009-01-07 | 赤水市金斛产业开发有限公司 | Dendrobium nobi tissue culturing and planting method |
CN101444181A (en) * | 2008-12-19 | 2009-06-03 | 查应洪 | Method for growing dendrobium |
CN101695249A (en) * | 2009-10-26 | 2010-04-21 | 重庆市中药研究院 | Non-tube rapid propagation method for dendrobium devonianum |
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2010
- 2010-10-21 CN CN2010105143535A patent/CN101999307B/en not_active Expired - Fee Related
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN101336605A (en) * | 2007-07-07 | 2009-01-07 | 赤水市金斛产业开发有限公司 | Dendrobium nobi tissue culturing and planting method |
CN101444181A (en) * | 2008-12-19 | 2009-06-03 | 查应洪 | Method for growing dendrobium |
CN101695249A (en) * | 2009-10-26 | 2010-04-21 | 重庆市中药研究院 | Non-tube rapid propagation method for dendrobium devonianum |
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102440130A (en) * | 2011-09-22 | 2012-05-09 | 澄思源生物科技(上海)有限公司 | Method for natural propagation of dendrobium officinale seeds |
CN103168667A (en) * | 2011-12-23 | 2013-06-26 | 何寒 | Rapid propagation technology of Dendrobium officinale |
CN103004445A (en) * | 2013-01-06 | 2013-04-03 | 戴亚峰 | Method for directly seedling dendrobe seedlings by using plastic greenhouse |
CN103931410A (en) * | 2014-05-06 | 2014-07-23 | 龙陵县云河石斛开发有限责任公司 | Open forest seedling cultivation method for Den. devonianum Paxt |
CN106577232A (en) * | 2016-12-13 | 2017-04-26 | 重庆市中药研究院 | Natural propagation method for sowing seeds of dendrobium flexicaule on palisades |
CN106577232B (en) * | 2016-12-13 | 2020-07-24 | 重庆市中药研究院 | Natural propagation method for rock wall sowing of dendrobium aspergillum seeds |
CN108782199A (en) * | 2018-05-19 | 2018-11-13 | 安徽省霍山县霍鼎仙石斛开发有限公司 | A kind of implantation methods of growing nonparasitically upon another plant of the stem of noble dendrobium |
CN111616038A (en) * | 2020-05-20 | 2020-09-04 | 龙陵县石斛研究所 | Dendrobium devonianum seedling growing method |
CN112021168A (en) * | 2020-09-25 | 2020-12-04 | 广东永生源生物科技有限公司 | Method for planting dendrobium officinale by using straw ropes to simulate wild trees |
CN115005073A (en) * | 2022-06-22 | 2022-09-06 | 中国热带农业科学院热带作物品种资源研究所 | Dendrobium autumn open seeding method based on napkin |
CN115005073B (en) * | 2022-06-22 | 2024-01-09 | 中国热带农业科学院热带作物品种资源研究所 | Open autumn dendrobium stem sowing method based on dining paper towel |
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