CN103168667A - Rapid propagation technology of Dendrobium officinale - Google Patents
Rapid propagation technology of Dendrobium officinale Download PDFInfo
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- CN103168667A CN103168667A CN201110440089XA CN201110440089A CN103168667A CN 103168667 A CN103168667 A CN 103168667A CN 201110440089X A CN201110440089X A CN 201110440089XA CN 201110440089 A CN201110440089 A CN 201110440089A CN 103168667 A CN103168667 A CN 103168667A
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- dendrobium candidum
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Abstract
The invention relates to the technical field of plant vegetative propagation, in particular to rapid propagation technology of Dendrobium officinale. The rapid propagation technology of the Dendrobium officinale mainly comprises establishing a seedbed with sawdust serving as a substrate of the seedbed, collecting, manufacturing and processing separation materials of the Dendrobium officinale, a selecting method, a preparing method and a using method of a hormone and a management method of the seedbed in the process of seed cultivation. The substrate selected by the rapid propagation technology is good in air permeability, good in moisture retention, good in affinity with the Dendrobium officinale, wide in raw material resources and low in cost. In the process of production management, an automation degree is high, designed humidity control is scientific and reasonable, a control range is accurate and an atomization effect is good and humidity is even in the process of water spraying. The rapid propagation technology of the Dendrobium officinale is simple in process, easy to operate, short in seed cultivation period and high in survival rate.
Description
Technical field
The present invention relates to the plant asexual multiplication technology field, be specifically related to a kind of fast breeding technique of dendrobium candidum.
Technical background
Dendrobium candidum claims again ribbed hedyotis herb, belongs to the orchid family Dendrobium, is a kind of Wild Medicinal of preciousness.Its active ingredient is mainly dendrobium polysaccharide, dendrobine, amino acid etc., and wherein dendrobium polysaccharide content up to 21.7%, can significantly improve body's immunity, has nourishing the stomach and goes out Tianjin, kidney tonifying benefit power, nourishing Yin and clearing heat, anti-anoxic, antifatigue, the effect such as antitumor.Modern pharmacology research discovery, the stem of noble dendrobium also has anti-ageing and effect hemangiectasis.
The environment that the dendrobium candidum happiness is shady and cool, moistening often is attached in the interior trunk of high height above sea level overhanging cliff or woods and rock crevice.High due to its medical value, growth conditions is special and distribution is limited to, and through long-term people for excavating, wild resource is endangered, thereby the domestic market supply shortage, international market per kilogram dry product valency has reached more than 1000 dollars.
Dendrobium candidum natural propagation rate is extremely low, mainly cultivates by tissue the problem that the amount reproduction candidum tissue culturing seedling solves seedling at present.But plant tissue culture technique is that technical very strong work, an infrastructure have high input, and aseptic condition requires high, and the hardening cycle is long, process is loaded down with trivial details.After the group training transplantation of seedlings of simultaneously, cultivating out by tissue is transplanted, survival rate only has at most 60%.
Application number is 200810021790.6 " a kind of cottage breeding method of dendrobium candidum ", and this invention comprises the steps: 1. to prepare the cuttage material: with pseudobulb from the foundation of parent cut off, as the cuttage material; 2. cuttage material segmentation: pseudobulb is cut into plugged ear, 2-3 joint is arranged on each plugged ear: the blade of internode is cut; After being soaked in liquor potassic permanganate, plugged ear dries; 3. cuttage plugged ear: plugged ear is inserted in ready cutting medium, water once permeable, the nonwoven that upper cover one deck is wet; 4. manage plugged ear: make culture environment be in half the moon, dampness, temperature is 5 ℃ of-35 ℃ of scopes, and illumination is in the 1500-5000lux scope; 5. transplant: cuttage 3～4 months afterwards, with the scissors of sterilizing, new plant is sheared off, transplant to transplanting medium.Utilize the plant of this invention bred can preserve completely the fine quality of parent, can not morph, cultivate achievement and can predict, reduced the risk of cultivating.This invention has been cast aside technical very strong, plant tissue culture technique that infrastructure has high input, the hardening cycle is long and has been realized that the amplification of dendrobium candidum breeds, but this invention automation for production management degree is low, the cycle that the dendrobium candidum seedling is cultivated needed 3～4 months, the long survival rate that has increased virtually the production management cost and can reduce product of cycle.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art and the fast breeding technique of brand-new a kind of dendrobium candidum is provided, specifically comprise the foundation in seedbed, do medium of seedling bed with wooden chaff, the collection of dendrobium candidum To body material, making and processing, hormone is selected, preparation and using method, the management method in seedbed when growing seedlings.
The present invention is achieved through the following technical solutions:
1, a kind of fast breeding technique of dendrobium candidum, it comprises the foundation in seedbed, does medium of seedling bed with wooden chaff, the collection of dendrobium candidum To body material, making and processing, hormone is selected, preparation and using method, the management method in seedbed when growing seedlings.
2, a kind of fast breeding technique of dendrobium candidum, the method for building up in its seedbed is as follows:
(1) selective light is according to sufficient, and the seedbed is built on draining good ground, and the seedbed specification is built furrow by long 10m, wide 1～1.2m, builds the edge of bed of high 15～20cm with brick on the furrow limits, slightly stays the gap in order to the discharge of water between brick and brick, stays the aisle of 40～50cm between the seedbed.
(2) wherein a kind of such as the wooden chaff of the wooden chaff of medium of seedling bed choice for use pine, China fir, the wooden chaff of various weedtrees, the matrix laying depth is 15～20cm.
(3) micro-nozzle road on frame above seedbed center, micro-nozzle road are 60～70cm far from bed surface, at interval of 50cm, an atomizer are installed on pipeline, and the direction that shower nozzle is installed is vertically upward.
(4) the micro-nozzle road is connecting water pump, during work is to be supplied water by water pump, fills the air at air and spray and form the water atomization shape from atomizer by pipeline under the pressure that water produces when water pump supplies water, thereby reaches needed humidity requirement in seedling raising environment.
(5) water pump be by the time relay or intermittently time controller control, the time relay or intermittently the effect of time controller be when to spray water for setting by the people, after the data of spray water how long, the time relay or intermittently time controller will be that the parameter that sets is controlled water pump and carried out the work water spray according to the people.
(6) establish tank at the head of a bed in seedbed, water pump is placed in tank.
3, a kind of fast breeding technique of dendrobium candidum, the collection of dendrobium candidum To body material, making and processing method are as follows:
(1) To body material collection: select growth vigorous, without the dendrobium candidum of damage by disease and insect as female parent.
(2) making of To body material: adopt sharp pruning shear, grafting knife or blade, dendrobium candidum is cut (cutting) becomes that two buds are above, four buds are with interior branch section.
(3) process: with the dendrobium candidum To body material with base portion down, the classification of transplanting a cutting is by size got well and lashes, and neat concentrated being placed in plastic basin poured clear water in basin into to flooding the To body material base portion, soaked 3～4 days, and can effectively remove the obstruction material of taking root; During this time, changed one time clear water in every 12 hours.
4, a kind of fast breeding technique of dendrobium candidum, hormone is selected, preparation and using method as follows:
(1) selecting of hormone: select the ABT1 root-inducing powder.
(2) preparation of hormone: 1 gram ABT1 root-inducing powder is first after 75% medicinal alcohol comes dilution with 500ml content, then adds clear water and be dissolved to 10000ml.
(3) using method: will soak the dendrobium candidum To body material of clear water with base portion down, neat concentrated being placed in plastic basin poured the hormone for preparing in basin into to flooding the To body material base portion, and soak time is 3～4 hours.
5, a kind of fast breeding technique of dendrobium candidum, the dendrobium candidum To body material is as follows in the management method in seedbed:
(1) field planting: the dendrobium candidum To body material is evenly inserted the seedbed by the spacing of 2～5cm between strain and strain.
(2) water spray management: the mist frequency of the To body material of dendrobium candidum spraying before callus forms is every 2 hours water sprays 10～15 seconds, the frequency of spraying after the former base generation of the To body material root of dendrobium candidum changes every 6 hours into and sprayed water 8～10 seconds, and the longer rear spraying frequency of the coring of dendrobium candidum To body material changes every 12 hours into and sprayed water 8～10 seconds; To evening or overcast and rainyly can cut off the control water system.
(3) foliar application: after the generation of the former base of To body material root of dendrobium candidum, spraying concentration is 2～3% organic foliar fertilizers, sprays once in every 7 days, until the To body material seedling of dendrobium candidum and transplanting.
(4) transplant: after 50～60 days, the root system of the To body material of dendrobium candidum is grown, and can transplant to nutrition cup (bag).
Compared with prior art, the existing following advantage of the present invention:
1, selected matrix good permeability, the performance of keeping humidity of the present invention good, with dendrobium candidum, good compatibility is arranged, raw material sources are wide, cheap.
2, automaticity is high during production management of the present invention, has not only improved thus operating efficiency, can also therefrom reduce the labour.
3, the designed humidity of the present invention is controlled scientific and reasonablely, and control range is accurate, and during water spray, atomizing effect is good, humidity is balanced.
4, the present invention is simple for process, breeds that the seedling cycle is short, survival rate is high.
5, technique of the present invention is simple, and effect is remarkable, easily penetration and promotion.
Embodiment
Below in conjunction with embodiment, method of the present invention is further illustrated.
1, a kind of fast breeding technique of dendrobium candidum, it comprises the foundation in seedbed, does medium of seedling bed with wooden chaff, the collection of dendrobium candidum To body material, making and processing, hormone is selected, preparation and using method, the management method in seedbed when growing seedlings.
2, a kind of fast breeding technique of dendrobium candidum, the method for building up in its seedbed is as follows:
(1) selective light is according to sufficient, and the seedbed is built on draining good ground, and the seedbed specification is built furrow by long 10m, wide 1～1.2m, builds the edge of bed of high 15～20cm with brick on the furrow limits, slightly stays the gap in order to the discharge of water between brick and brick, stays the aisle of 40～50cm between the seedbed.
(2) wherein a kind of such as the wooden chaff of the wooden chaff of medium of seedling bed choice for use pine, China fir, the wooden chaff of various weedtrees, the matrix laying depth is 15～20cm.
(3) micro-nozzle road on frame above seedbed center, micro-nozzle road are 60～70cm far from bed surface, at interval of 50cm, an atomizer are installed on pipeline, and the direction that shower nozzle is installed is vertically upward.
(4) the micro-nozzle road is connecting water pump, during work is to be supplied water by water pump, fills the air at air and spray and form the water atomization shape from atomizer by pipeline under the pressure that water produces when water pump supplies water, thereby reaches needed humidity requirement in seedling raising environment.
(5) water pump be by the time relay or intermittently time controller control, the time relay or intermittently the effect of time controller be when to spray water for setting by the people, after the data of spray water how long, the time relay or intermittently time controller will be that the parameter that sets is controlled water pump and carried out the work water spray according to the people.
(6) establish tank at the head of a bed in seedbed, water pump is placed in tank.
3, a kind of fast breeding technique of dendrobium candidum, the collection of dendrobium candidum To body material, making and processing method are as follows:
(1) To body material collection: select growth vigorous, without the dendrobium candidum of damage by disease and insect as female parent.
(2) making of To body material: adopt sharp pruning shear, grafting knife or blade, dendrobium candidum is cut (cutting) becomes that two buds are above, four buds are with interior branch section.
(3) process: with the dendrobium candidum To body material with base portion down, the classification of transplanting a cutting is by size got well and lashes, and neat concentrated being placed in plastic basin poured clear water in basin into to flooding the To body material base portion, soaked 3～4 days, and can effectively remove the obstruction material of taking root; During this time, changed one time clear water in every 12 hours.
4, a kind of fast breeding technique of dendrobium candidum, hormone is selected, preparation and using method as follows:
(1) selecting of hormone: select the ABT1 root-inducing powder.
(2) preparation of hormone: 1 gram ABT1 root-inducing powder is first after 75% medicinal alcohol comes dilution with 500ml content, then adds clear water and be dissolved to 10000ml.
(3) using method: will soak the dendrobium candidum To body material of clear water with base portion down, neat concentrated being placed in plastic basin poured the hormone for preparing in basin into to flooding the To body material base portion, and soak time is 3～4 hours.
5, a kind of fast breeding technique of dendrobium candidum, the dendrobium candidum To body material is as follows in the management method in seedbed:
(1) field planting: the dendrobium candidum To body material is evenly inserted the seedbed by the spacing of 2～5cm between strain and strain.
(2) water spray management: the mist frequency of the To body material of dendrobium candidum spraying before callus forms is every 2 hours water sprays 10～15 seconds, the frequency of spraying after the former base generation of the To body material root of dendrobium candidum changes every 6 hours into and sprayed water 8～10 seconds, and the longer rear spraying frequency of the coring of dendrobium candidum To body material changes every 12 hours into and sprayed water 8～10 seconds; To evening or overcast and rainyly can cut off the control water system.
(3) foliar application: after the generation of the former base of To body material root of dendrobium candidum, spraying concentration is 2～3% organic foliar fertilizers, sprays once in every 7 days, until the To body material seedling of dendrobium candidum and transplanting.
(4) transplant: after 50～60 days, the root system of the To body material of dendrobium candidum is grown, and can transplant to nutrition cup (bag).
Claims (5)
1. the fast breeding technique of a dendrobium candidum, its feature comprises: the foundation in seedbed, do medium of seedling bed with wooden chaff, the collection of dendrobium candidum To body material, making and processing, hormone is selected, preparation and using method, the management method in seedbed when growing seedlings.
2. the fast breeding technique of a kind of dendrobium candidum described according to claim 1 is characterized in that the method for building up in seedbed is as follows:
(1) selective light is according to sufficient, and the seedbed is built on draining good ground, and the seedbed specification is built furrow by long 10m, wide 1～1.2m, builds the edge of bed of high 15～20cm with brick on the furrow limits, slightly stays the gap in order to the discharge of water between brick and brick, stays the aisle of 40～50cm between the seedbed.
(2) wherein a kind of such as the wooden chaff of the wooden chaff of medium of seedling bed choice for use pine, China fir, the wooden chaff of various weedtrees, the matrix laying depth is 15～20cm.
(3) micro-nozzle road on frame above seedbed center, micro-nozzle road are 60～70cm far from bed surface, at interval of 50cm, an atomizer are installed on pipeline, and the direction that shower nozzle is installed is vertically upward.
(4) the micro-nozzle road is connecting water pump, during work is to be supplied water by water pump, fills the air at air and spray and form the water atomization shape from atomizer by pipeline under the pressure that water produces when water pump supplies water, thereby reaches needed humidity requirement in seedling raising environment.
(5) water pump be by the time relay or intermittently time controller control, the time relay or intermittently the effect of time controller be when to spray water for setting by the people, after the data of spray water how long, the time relay or intermittently time controller will be that the parameter that sets is controlled water pump and carried out the work water spray according to the people.
(6) establish tank at the head of a bed in seedbed, water pump is placed in tank.
3. the fast breeding technique of a kind of dendrobium candidum described according to claim 1 is characterized in that the collection of dendrobium candidum To body material, making and processing method are as follows:
(1) To body material collection: select growth vigorous, without the dendrobium candidum of damage by disease and insect as female parent.
(2) making of To body material: adopt sharp pruning shear, grafting knife or blade, dendrobium candidum is cut (cutting) becomes that two buds are above, four buds are with interior branch section.
(3) process: with the dendrobium candidum To body material with base portion down, the classification of transplanting a cutting is by size got well and lashes, and neat concentrated being placed in plastic basin poured clear water in basin into to flooding the To body material base portion, soaked 3～4 days, and can effectively remove the obstruction material of taking root; During this time, changed one time clear water in every 12 hours.
4. the fast breeding technique of a kind of dendrobium candidum described according to claim 1, it is characterized in that hormone is selected, preparation and using method as follows:
(1) selecting of hormone: select the ABT1 root-inducing powder.
(2) preparation of hormone: 1 gram ABT1 root-inducing powder is first after 75% medicinal alcohol comes dilution with 500ml content, then adds clear water and be dissolved to 10000ml.
(3) using method: will soak the dendrobium candidum To body material of clear water with base portion down, neat concentrated being placed in plastic basin poured the hormone for preparing in basin into to flooding the To body material base portion, and soak time is 3～4 hours.
5. the fast breeding technique of a kind of dendrobium candidum described according to claim 1 is characterized in that the dendrobium candidum To body material is as follows in the management method in seedbed:
(1) field planting: the dendrobium candidum To body material is evenly inserted the seedbed by the spacing of 2～5cm between strain and strain.
(2) water spray management: the mist frequency of the To body material of dendrobium candidum spraying before callus forms is every 2 hours water sprays 10～15 seconds, the frequency of spraying after the former base generation of the To body material root of dendrobium candidum changes every 6 hours into and sprayed water 8～10 seconds, and the longer rear spraying frequency of the coring of dendrobium candidum To body material changes every 12 hours into and sprayed water 8～10 seconds; To evening or overcast and rainyly can cut off the control water system.
(3) foliar application: after the generation of the former base of To body material root of dendrobium candidum, spraying concentration is 2～3% organic foliar fertilizers, sprays once in every 7 days, until the To body material seedling of dendrobium candidum and transplanting.
(4) transplant: after 50～60 days, the root system of the To body material of dendrobium candidum is grown, and can transplant to nutrition cup (bag).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110440089XA CN103168667A (en) | 2011-12-23 | 2011-12-23 | Rapid propagation technology of Dendrobium officinale |
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| CN201110440089XA CN103168667A (en) | 2011-12-23 | 2011-12-23 | Rapid propagation technology of Dendrobium officinale |
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| CN103168667A true CN103168667A (en) | 2013-06-26 |
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| CN201110440089XA Pending CN103168667A (en) | 2011-12-23 | 2011-12-23 | Rapid propagation technology of Dendrobium officinale |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101999307A (en) * | 2010-10-21 | 2011-04-06 | 重庆市中药研究院 | Natural reproduction method for sowing dendrobium on boards and barks |
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- 2011-12-23 CN CN201110440089XA patent/CN103168667A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101999307A (en) * | 2010-10-21 | 2011-04-06 | 重庆市中药研究院 | Natural reproduction method for sowing dendrobium on boards and barks |
Non-Patent Citations (1)
| Title |
|---|
| 罗太洪: "石斛快速简便扦插繁育新方法", 《农技服务》, no. 1, 31 January 2007 (2007-01-31), pages 21 * |
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Application publication date: 20130626 |