CN101868536B - 从人脐带分离和保存前体细胞的优化且确定的方法 - Google Patents

从人脐带分离和保存前体细胞的优化且确定的方法 Download PDF

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Publication number
CN101868536B
CN101868536B CN200880114557.0A CN200880114557A CN101868536B CN 101868536 B CN101868536 B CN 101868536B CN 200880114557 A CN200880114557 A CN 200880114557A CN 101868536 B CN101868536 B CN 101868536B
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cell
cells
umbilical cord
digestion
days
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CN101868536A (zh
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R·I·冈查斯索雷斯
M·C·巴普提斯塔克尔豪
J·M·希尔瓦桑图斯
J·P·玛丁斯
V·A·巴斯图
P·埃斯提利塔蒙提罗达克鲁兹
H·J·索雷斯达克鲁兹
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MEDINFAR PRODUTOS FARMACEUTICO
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/44—Vessels; Vascular smooth muscle cells; Endothelial cells; Endothelial progenitor cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0604—Whole embryos; Culture medium therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0668—Mesenchymal stem cells from other natural sources
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Gynecology & Obstetrics (AREA)
  • Cell Biology (AREA)
  • Reproductive Health (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Rheumatology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Vascular Medicine (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Epidemiology (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
  • Cosmetics (AREA)
CN200880114557.0A 2007-10-04 2008-10-03 从人脐带分离和保存前体细胞的优化且确定的方法 Expired - Fee Related CN101868536B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
PT103843A PT103843B (pt) 2007-10-04 2007-10-04 Método de isolamento de células precursoras a partir do cordão umbilical humano
PT103843 2007-10-04
PCT/IB2008/054067 WO2009044379A2 (en) 2007-10-04 2008-10-03 Optimised and defined method for isolation and preservation of precursor cells from human umbilical cord

Publications (2)

Publication Number Publication Date
CN101868536A CN101868536A (zh) 2010-10-20
CN101868536B true CN101868536B (zh) 2015-03-25

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CN200880114557.0A Expired - Fee Related CN101868536B (zh) 2007-10-04 2008-10-03 从人脐带分离和保存前体细胞的优化且确定的方法

Country Status (15)

Country Link
US (1) US9546353B2 (enExample)
EP (1) EP2203555B1 (enExample)
JP (1) JP5785394B2 (enExample)
KR (1) KR20100087705A (enExample)
CN (1) CN101868536B (enExample)
AU (1) AU2008306442B2 (enExample)
BR (1) BRPI0817522A2 (enExample)
CA (1) CA2701907A1 (enExample)
EA (1) EA025532B1 (enExample)
ES (1) ES2582369T3 (enExample)
IL (1) IL204788A (enExample)
MX (1) MX2010003511A (enExample)
PT (1) PT103843B (enExample)
WO (1) WO2009044379A2 (enExample)
ZA (1) ZA201002260B (enExample)

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Publication number Priority date Publication date Assignee Title
ES2634545T3 (es) 2010-06-01 2017-09-28 Auxocell Laboratories, Inc. Células madre nativas de la gelatina de Wharton y su purificación
US9234177B2 (en) 2010-10-18 2016-01-12 Sunshine Life Science & Technology Corp. Human multipotent embryonic stem cell-like progenitor cells
TWI535377B (zh) * 2011-09-01 2016-06-01 Storage, culture and application of umbilical cord tissue and its derived cells
ES2890126T3 (es) * 2011-12-30 2022-01-17 Amit Patel Métodos y composiciones para la obtención clínica de una célula alogénica y usos terapéuticos
CN102550543B (zh) * 2012-02-10 2014-04-16 四川新生命干细胞科技股份有限公司 用于保存脐带组织的液体
CN103461322B (zh) * 2012-06-07 2016-08-03 西比曼生物科技（上海）有限公司 用于冻存复苏后的细胞的保存液
EP2756754B1 (de) 2013-01-17 2017-01-04 Vita 34 Ag Verfahren zur Behandlung von Nabelschnurgewebe, insbesondere im Zusammenhang mit der Konservierung des Gewebes
WO2015181831A2 (en) * 2014-05-24 2015-12-03 Venkataramanaa Neelamkrishnan A method for targettingglioblastoma with wharton jelly-mesenchymal stem cells (wj-msc) derived from human umbilical cord
JP2017533917A (ja) * 2014-11-05 2017-11-16 ティッシュテック，インク． 神経成長および再生を促進するための組成物および方法
WO2017004460A1 (en) * 2015-06-30 2017-01-05 Surgenex, LLC Compositions and methods for flowable allograft tissue
WO2017096611A1 (zh) * 2015-12-11 2017-06-15 郭镭 一种从脐带华通氏胶组织中分离培养脐带间充质干细胞的方法
WO2019175773A1 (en) 2018-03-12 2019-09-19 Universidade Do Porto Compositions for use in the treatment of musculoskeletal conditions and methods for producing the same leveraging the synergistic activity of two different types of mesenchymal stromal/stem cells
CN112342191A (zh) * 2019-08-07 2021-02-09 路春光 hMSC生产试剂盒开发和质量管理标准优化和体系建立
CN111893092A (zh) * 2020-06-28 2020-11-06 国大生命科学产业集团(深圳)有限公司 一种人脐带来源的间充质干细胞及其制备方法
CN113025567A (zh) * 2021-03-31 2021-06-25 中国人民解放军陆军特色医学中心 一种椎间盘单细胞的分离方法
DE102021116694B4 (de) * 2021-06-29 2023-03-09 Promocell Gmbh Wässrige Lösung zur Zellkonservierung

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AT402890B (de) * 1994-03-16 1997-09-25 Berbi Gmbh Verwendung von notoginsenosid r1 zur herstellung von arzneimitteln
JPWO2003080822A1 (ja) * 2002-03-27 2005-07-28 ニプロ株式会社 胎盤由来の間葉系細胞およびその医学的用途
CA2633980A1 (en) * 2005-12-29 2007-07-12 Anthrogenesis Corporation Improved composition for collecting and preserving placental stem cells and methods of using the composition
ES2524443T3 (es) * 2006-11-13 2014-12-09 DePuy Synthes Products, LLC Expansión in vitro de células postparto usando microportadores

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
"Human Umbilical Cord Matrix Stem Cells: Preliminary Characterization and Effect of Transplantation in a Rodent Model of Parkinson’s Disease";MARK L. WEISS. et al.;《Stem Cell》;20060331;第24卷(第3期);第781页摘要 *
ALP CAN et al.."Concise Review: Human Umbilical Cord Stroma with Regard to the Source of Fetus-Derived Stem Cells".《Stem Cell》.2007,第25卷(第11期),第2892页右栏第3段-第2893页右栏第2段. *
Lu-Lu Lu et al.."Isolation and characterization of human umbilical cord mesenchymal stem cells with hematopoiesis-supportive function and other potentials".《Hematopoietic Stem Cells》.2006,第91卷(第8期),第1024页左栏最后一段-右栏第1段，第1025页左栏最后一段-右栏第1段. *

Also Published As

Publication number Publication date
MX2010003511A (es) 2010-09-10
JP2010539970A (ja) 2010-12-24
JP5785394B2 (ja) 2015-09-30
AU2008306442B2 (en) 2014-12-04
EP2203555A2 (en) 2010-07-07
ZA201002260B (en) 2011-06-29
ES2582369T3 (es) 2016-09-12
EA201000425A1 (ru) 2010-10-29
KR20100087705A (ko) 2010-08-05
IL204788A (en) 2013-07-31
EP2203555B1 (en) 2016-04-13
US20100216237A1 (en) 2010-08-26
PT103843A (pt) 2008-01-09
PT103843B (pt) 2008-08-12
CA2701907A1 (en) 2009-04-09
US9546353B2 (en) 2017-01-17
WO2009044379A2 (en) 2009-04-09
EA025532B1 (ru) 2017-01-30
WO2009044379A3 (en) 2009-05-22
CN101868536A (zh) 2010-10-20
IL204788A0 (en) 2010-12-30
AU2008306442A1 (en) 2009-04-09
BRPI0817522A2 (pt) 2014-10-14

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