EA201000425A1 - Оптимизированный и определенный способ выделения и консервации клеток-предшественников из человеческой пуповины - Google Patents

Оптимизированный и определенный способ выделения и консервации клеток-предшественников из человеческой пуповины

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Publication number
EA201000425A1
EA201000425A1 EA201000425A EA201000425A EA201000425A1 EA 201000425 A1 EA201000425 A1 EA 201000425A1 EA 201000425 A EA201000425 A EA 201000425A EA 201000425 A EA201000425 A EA 201000425A EA 201000425 A1 EA201000425 A1 EA 201000425A1
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EA
Eurasian Patent Office
Prior art keywords
cells
human
umbilical cord
optimized
isolation
Prior art date
Application number
EA201000425A
Other languages
English (en)
Other versions
EA025532B1 (ru
Inventor
Рита Изабел Ганшаш Соарес
Мария Констанса Баптиста Коэльо
Жоржи Мигел Силва Сантош
Жозе Пауло Мартинш
Вера Александра Басто
Педро Эстилита Монтейро Да Круз
Эльдер Жуаким Соареш Да Круз
Original Assignee
Лабораторио Мединфар-Продутос Фармасеутикос, С.А.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Лабораторио Мединфар-Продутос Фармасеутикос, С.А. filed Critical Лабораторио Мединфар-Продутос Фармасеутикос, С.А.
Publication of EA201000425A1 publication Critical patent/EA201000425A1/ru
Publication of EA025532B1 publication Critical patent/EA025532B1/ru

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0668—Mesenchymal stem cells from other natural sources
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Rheumatology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
  • Cosmetics (AREA)

Abstract

Настоящее изобретение относится к оптимизированному и упрощенному способу выделения и консервации клеток-предшественников из человеческой пуповины. Помимо воспроизводимости и 100%-ной надежности в показателях числа переработанных проб данный способ дает в результате большое и определенное число клеток-предшественников, являющихся в своем большинстве клетками, полученными после одной фазы прикрепления и роста/размножения ex vivo (таким образом дающей фенотип клетки), за более короткий период, чем это было описано ранее в предшествующем уровне техники. При помощи этого способа за 9 суток после непосредственного замораживания фракции клеток и после одной фазы роста/размножения большинства клеток ех vivo (конец Р0) можно получить примерно 8,6(±0,1)×10клеток/г переработанной пуповины. В свою очередь, характеристики клеток позволяют, например, через 35 суток получить в среднем 7,7×10клеток с фенотипом предшественников из 100% переработанных проб пуповины. Данный способ, поскольку он является простым, функциональным и на 100% надежным, можно осуществлять согласно правилам организации производства и контроля качества лекарственных средств (GMP) в лабораториях, специализирующихся на клеточной терапии человека. Кроме того, данный способ имеет применения в фармацевтических, косметических и биотехнологических областях.
EA201000425A 2007-10-04 2008-10-03 Способ выделения клеток-предшественников из человеческой пуповины EA025532B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PT103843A PT103843B (pt) 2007-10-04 2007-10-04 Método de isolamento de células precursoras a partir do cordão umbilical humano
PCT/IB2008/054067 WO2009044379A2 (en) 2007-10-04 2008-10-03 Optimised and defined method for isolation and preservation of precursor cells from human umbilical cord

Publications (2)

Publication Number Publication Date
EA201000425A1 true EA201000425A1 (ru) 2010-10-29
EA025532B1 EA025532B1 (ru) 2017-01-30

Family

ID=40456372

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201000425A EA025532B1 (ru) 2007-10-04 2008-10-03 Способ выделения клеток-предшественников из человеческой пуповины

Country Status (15)

Country Link
US (1) US9546353B2 (ru)
EP (1) EP2203555B1 (ru)
JP (1) JP5785394B2 (ru)
KR (1) KR20100087705A (ru)
CN (1) CN101868536B (ru)
AU (1) AU2008306442B2 (ru)
BR (1) BRPI0817522A2 (ru)
CA (1) CA2701907A1 (ru)
EA (1) EA025532B1 (ru)
ES (1) ES2582369T3 (ru)
IL (1) IL204788A (ru)
MX (1) MX2010003511A (ru)
PT (1) PT103843B (ru)
WO (1) WO2009044379A2 (ru)
ZA (1) ZA201002260B (ru)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL2576768T3 (pl) 2010-06-01 2017-10-31 Auxocell Laboratories Inc Oczyszczanie niehodowanych komórek macierzystych galarety Whartona
WO2012054501A1 (en) * 2010-10-18 2012-04-26 Sunshine Biotech Inc. Human multipotent embryonic stem cell-like progenitor cells
TWI535377B (zh) * 2011-09-01 2016-06-01 Storage, culture and application of umbilical cord tissue and its derived cells
ES2656614T3 (es) * 2011-12-30 2018-02-27 Amit Patel Métodos y composiciones para la derivación clínica de células alogénicas y sus usos terapéuticos
CN102550543B (zh) * 2012-02-10 2014-04-16 四川新生命干细胞科技股份有限公司 用于保存脐带组织的液体
CN103461322B (zh) * 2012-06-07 2016-08-03 西比曼生物科技（上海）有限公司 用于冻存复苏后的细胞的保存液
EP2756754B1 (de) 2013-01-17 2017-01-04 Vita 34 Ag Verfahren zur Behandlung von Nabelschnurgewebe, insbesondere im Zusammenhang mit der Konservierung des Gewebes
EP3149157A4 (en) * 2014-05-24 2018-06-13 Neelamkrishnan Venkataramanaa A method for targettingglioblastoma with wharton jelly-mesenchymal stem cells (wj-msc) derived from human umbilical cord
US20160120912A1 (en) * 2014-11-05 2016-05-05 Tissuetech, Inc. Compositions and method for promoting nerve growth and regeneration
US10006003B2 (en) * 2015-06-30 2018-06-26 Surgenex, LLC Compositions and methods for flowable allograft amniotic tissue
EP3388512B1 (en) * 2015-12-11 2023-08-23 Lei Guo Method for separating and culturing mesenchymal stem cells from wharton's jelly tissue of umbilical cord
WO2019175773A1 (en) 2018-03-12 2019-09-19 Universidade Do Porto Compositions for use in the treatment of musculoskeletal conditions and methods for producing the same leveraging the synergistic activity of two different types of mesenchymal stromal/stem cells
CN112342191A (zh) * 2019-08-07 2021-02-09 路春光 hMSC生产试剂盒开发和质量管理标准优化和体系建立
CN111893092A (zh) * 2020-06-28 2020-11-06 国大生命科学产业集团(深圳)有限公司 一种人脐带来源的间充质干细胞及其制备方法
CN113025567A (zh) * 2021-03-31 2021-06-25 中国人民解放军陆军特色医学中心 一种椎间盘单细胞的分离方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT402890B (de) * 1994-03-16 1997-09-25 Berbi Gmbh Verwendung von notoginsenosid r1 zur herstellung von arzneimitteln
WO2003080822A1 (fr) * 2002-03-27 2003-10-02 Nipro Corporation Cellules mesenchymales placentaires et leur utilisation medicale
JP2009521931A (ja) * 2005-12-29 2009-06-11 アントフロゲネシス コーポレーション 胎盤幹細胞を収集及び保存するための改善された組成物、及び該組成物の使用方法
US9102915B2 (en) * 2006-11-13 2015-08-11 DePuy Synthes Products, Inc. In vitro expansion of postpartum-derived cells using microcarriers

Also Published As

Publication number Publication date
US20100216237A1 (en) 2010-08-26
PT103843A (pt) 2008-01-09
EA025532B1 (ru) 2017-01-30
CN101868536A (zh) 2010-10-20
ES2582369T3 (es) 2016-09-12
CN101868536B (zh) 2015-03-25
US9546353B2 (en) 2017-01-17
WO2009044379A3 (en) 2009-05-22
IL204788A0 (en) 2010-12-30
IL204788A (en) 2013-07-31
EP2203555A2 (en) 2010-07-07
BRPI0817522A2 (pt) 2014-10-14
WO2009044379A2 (en) 2009-04-09
EP2203555B1 (en) 2016-04-13
AU2008306442A1 (en) 2009-04-09
JP5785394B2 (ja) 2015-09-30
AU2008306442B2 (en) 2014-12-04
MX2010003511A (es) 2010-09-10
CA2701907A1 (en) 2009-04-09
ZA201002260B (en) 2011-06-29
PT103843B (pt) 2008-08-12
KR20100087705A (ko) 2010-08-05
JP2010539970A (ja) 2010-12-24

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Designated state(s): AM AZ BY KZ KG MD TJ TM RU