CA2701907A1 - Optimized and defined method for isolation and preservation of precursor cells from human umbilical cord - Google Patents

Optimized and defined method for isolation and preservation of precursor cells from human umbilical cord Download PDF

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Publication number
CA2701907A1
CA2701907A1 CA2701907A CA2701907A CA2701907A1 CA 2701907 A1 CA2701907 A1 CA 2701907A1 CA 2701907 A CA2701907 A CA 2701907A CA 2701907 A CA2701907 A CA 2701907A CA 2701907 A1 CA2701907 A1 CA 2701907A1
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CA
Canada
Prior art keywords
cells
cell
umbilical cord
digestion
optimized
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2701907A
Other languages
English (en)
French (fr)
Inventor
Rita Isabel Ganchas Soares
Maria Constanca Baptista Coelho
Jorge Miguel Silva Santos
Jose Paulo Martins
Vera Alexandra Basto
Pedro Estilita Monteiro Da Cruz
Helder Joaquim Soares Da Cruz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
LABORATORIO MEDINFAR-PRODUTOS FARMACEUTICOS SA
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2701907A1 publication Critical patent/CA2701907A1/en
Abandoned legal-status Critical Current

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Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10Preservation of living parts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0603Embryonic cells ; Embryoid bodies
    • C12N5/0605Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/44Vessels; Vascular smooth muscle cells; Endothelial cells; Endothelial progenitor cells
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0603Embryonic cells ; Embryoid bodies
    • C12N5/0604Whole embryos; Culture medium therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0603Embryonic cells ; Embryoid bodies
    • C12N5/0606Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0652Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662Stem cells
    • C12N5/0668Mesenchymal stem cells from other natural sources
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00Methods for the dissociation of cells, e.g. specific use of enzymes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Gynecology & Obstetrics (AREA)
  • Cell Biology (AREA)
  • Reproductive Health (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Rheumatology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Vascular Medicine (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Epidemiology (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
  • Cosmetics (AREA)
CA2701907A 2007-10-04 2008-10-03 Optimized and defined method for isolation and preservation of precursor cells from human umbilical cord Abandoned CA2701907A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
PT103843A PT103843B (pt) 2007-10-04 2007-10-04 Método de isolamento de células precursoras a partir do cordão umbilical humano
PT103843 2007-10-04
PCT/IB2008/054067 WO2009044379A2 (en) 2007-10-04 2008-10-03 Optimised and defined method for isolation and preservation of precursor cells from human umbilical cord

Publications (1)

Publication Number Publication Date
CA2701907A1 true CA2701907A1 (en) 2009-04-09

Family

ID=40456372

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2701907A Abandoned CA2701907A1 (en) 2007-10-04 2008-10-03 Optimized and defined method for isolation and preservation of precursor cells from human umbilical cord

Country Status (15)

Country Link
US (1) US9546353B2 (enExample)
EP (1) EP2203555B1 (enExample)
JP (1) JP5785394B2 (enExample)
KR (1) KR20100087705A (enExample)
CN (1) CN101868536B (enExample)
AU (1) AU2008306442B2 (enExample)
BR (1) BRPI0817522A2 (enExample)
CA (1) CA2701907A1 (enExample)
EA (1) EA025532B1 (enExample)
ES (1) ES2582369T3 (enExample)
IL (1) IL204788A (enExample)
MX (1) MX2010003511A (enExample)
PT (1) PT103843B (enExample)
WO (1) WO2009044379A2 (enExample)
ZA (1) ZA201002260B (enExample)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2634545T3 (es) 2010-06-01 2017-09-28 Auxocell Laboratories, Inc. Células madre nativas de la gelatina de Wharton y su purificación
US9234177B2 (en) 2010-10-18 2016-01-12 Sunshine Life Science & Technology Corp. Human multipotent embryonic stem cell-like progenitor cells
TWI535377B (zh) * 2011-09-01 2016-06-01 Storage, culture and application of umbilical cord tissue and its derived cells
ES2890126T3 (es) * 2011-12-30 2022-01-17 Amit Patel Métodos y composiciones para la obtención clínica de una célula alogénica y usos terapéuticos
CN102550543B (zh) * 2012-02-10 2014-04-16 四川新生命干细胞科技股份有限公司 用于保存脐带组织的液体
CN103461322B (zh) * 2012-06-07 2016-08-03 西比曼生物科技(上海)有限公司 用于冻存复苏后的细胞的保存液
EP2756754B1 (de) 2013-01-17 2017-01-04 Vita 34 Ag Verfahren zur Behandlung von Nabelschnurgewebe, insbesondere im Zusammenhang mit der Konservierung des Gewebes
WO2015181831A2 (en) * 2014-05-24 2015-12-03 Venkataramanaa Neelamkrishnan A method for targettingglioblastoma with wharton jelly-mesenchymal stem cells (wj-msc) derived from human umbilical cord
JP2017533917A (ja) * 2014-11-05 2017-11-16 ティッシュテック,インク. 神経成長および再生を促進するための組成物および方法
WO2017004460A1 (en) * 2015-06-30 2017-01-05 Surgenex, LLC Compositions and methods for flowable allograft tissue
WO2017096611A1 (zh) * 2015-12-11 2017-06-15 郭镭 一种从脐带华通氏胶组织中分离培养脐带间充质干细胞的方法
WO2019175773A1 (en) 2018-03-12 2019-09-19 Universidade Do Porto Compositions for use in the treatment of musculoskeletal conditions and methods for producing the same leveraging the synergistic activity of two different types of mesenchymal stromal/stem cells
CN112342191A (zh) * 2019-08-07 2021-02-09 路春光 hMSC生产试剂盒开发和质量管理标准优化和体系建立
CN111893092A (zh) * 2020-06-28 2020-11-06 国大生命科学产业集团(深圳)有限公司 一种人脐带来源的间充质干细胞及其制备方法
CN113025567A (zh) * 2021-03-31 2021-06-25 中国人民解放军陆军特色医学中心 一种椎间盘单细胞的分离方法
DE102021116694B4 (de) * 2021-06-29 2023-03-09 Promocell Gmbh Wässrige Lösung zur Zellkonservierung

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT402890B (de) * 1994-03-16 1997-09-25 Berbi Gmbh Verwendung von notoginsenosid r1 zur herstellung von arzneimitteln
JPWO2003080822A1 (ja) * 2002-03-27 2005-07-28 ニプロ株式会社 胎盤由来の間葉系細胞およびその医学的用途
CA2633980A1 (en) * 2005-12-29 2007-07-12 Anthrogenesis Corporation Improved composition for collecting and preserving placental stem cells and methods of using the composition
ES2524443T3 (es) * 2006-11-13 2014-12-09 DePuy Synthes Products, LLC Expansión in vitro de células postparto usando microportadores

Also Published As

Publication number Publication date
MX2010003511A (es) 2010-09-10
JP2010539970A (ja) 2010-12-24
JP5785394B2 (ja) 2015-09-30
AU2008306442B2 (en) 2014-12-04
EP2203555A2 (en) 2010-07-07
ZA201002260B (en) 2011-06-29
ES2582369T3 (es) 2016-09-12
EA201000425A1 (ru) 2010-10-29
KR20100087705A (ko) 2010-08-05
IL204788A (en) 2013-07-31
EP2203555B1 (en) 2016-04-13
US20100216237A1 (en) 2010-08-26
CN101868536B (zh) 2015-03-25
PT103843A (pt) 2008-01-09
PT103843B (pt) 2008-08-12
US9546353B2 (en) 2017-01-17
WO2009044379A2 (en) 2009-04-09
EA025532B1 (ru) 2017-01-30
WO2009044379A3 (en) 2009-05-22
CN101868536A (zh) 2010-10-20
IL204788A0 (en) 2010-12-30
AU2008306442A1 (en) 2009-04-09
BRPI0817522A2 (pt) 2014-10-14

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EEER Examination request

Effective date: 20130919

FZDE Discontinued

Effective date: 20181003