CN101816251B - Tetraploid locust cuttage breeding method - Google Patents
Tetraploid locust cuttage breeding method Download PDFInfo
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- CN101816251B CN101816251B CN2010101607954A CN201010160795A CN101816251B CN 101816251 B CN101816251 B CN 101816251B CN 2010101607954 A CN2010101607954 A CN 2010101607954A CN 201010160795 A CN201010160795 A CN 201010160795A CN 101816251 B CN101816251 B CN 101816251B
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Abstract
The invention discloses a tetraploid locust cuttage breeding method, which belongs to the field of ornamental plants. The method comprises the following steps of: soaking tetraploid locust hardwood branch segments in a plant growth regulator to perform sand storage in winter; performing yellowing pregermination on the sand-burying branch segments; dipping the germinated twigs in the plant growth regulator to perform cottage and rooting cultivation; and breeding the tetraploid locust nursery stocks. The tetraploid locust bred by the method has high cuttage and rooting efficiency; the cutting survival rate is obviously improved; and the method solves the technical problem of the cuttage propagation of the tetraploid locust hardwood branches.
Description
Technical field
The present invention relates to the asexual reproduction method of a kind of deciduous tree plant, particularly a kind of locust cuttage breeding method belongs to the ornamental plant field.
Background technology
Locust tree (Robinia pseudoacaia L) is a deciduous tree, and bark is thick, dead color, and line splits many; Flower is for white, and is savory, spike; Fruit is a pod, in each fruit pod 4-10 grain seed is arranged.This seeds happiness light, happiness warm and moist weather, not tight to the soil requirement, adaptability is very strong, likes deep, fertile, loose, the moistening silty soil of soil layer, sandy loam and loam most.Locust tree originates in the North America, and 17th century is just introduced Europe, and China is introduced at the 19th-century end.At present, spreaded all over North China, northwest, THE SOUTH OF NORTHEAST CHINA, cultivation has all been arranged in a plurality of areas of 23 °～46 ° of north latitude, 124 °～86 ° of east longitudes.
Locust tree is of many uses, has economic worth.Its tree crown is tall and big, and the leaf look bud green, sets each other off in vain whenever season of flowers is green, and is plain and fragrant, can be used as the pioneer tree species of street tree, shade tree, industrial and mining area greening and the greening of waste mountain waste; Its well developed root system, nodule nitrogen fixation can be used as the water and soil conservation seeds of checking winds and fixing drifting sand, improving the soil; Locust tree timber is hard, and is corrosion-resistant, and burning is slow, and calorific value is high, can supply ore pillar, sleeper, vehicle, agricultural to use material; Leaf contains crude protein, is the good feed of many domestic animals; Flower is good nectariferous plant, and the Pagoda Flower nectarine is white and transparent, and is very popular; Tender leaf is spent edible, has become city dweller's green vegetables at present; The seed oil expression supplies to do soap and paint raw materials.
Tetraploid locust is the locust tree choiceness kind that China introduces from Korea S, and this kind is an appliable plant ploidy breeding technology, through induced mutations, with locust tree somatic double gained.Because the huge effect of polyploid makes this kind show growth rapidly with respect to common locust tree, blade is loose, and biomass is big, the high characteristics of nutrition content such as protein, fat in the cauline leaf.The tetraploid locust growth is rapid, and well developed root system is flourishing; Low temperature resistant, arid, barren; Anti-saline and alkaline, disease and insect resistance has been generalized to more than 20 provinces in the whole nation at present; Be widely used in afforested projects such as feedstuff woods, the deserted mountain desert ecological forest and conceding the land to forestry, obtained good ecological benefits, economic benefit and social benefit.
After tetraploid locust is introduced China, there is the low problem of cuttage rooting rate and survival rate always.For solving the low difficult problem of cuttage survival rate, general at present employing tissue culture and grafting method carry out the vegetative propagation of tetraploid locust, and not only the propagation technique step is numerous and diverse for tissue culture and grafting method, and survival rate is still lower.For example: the tetraploid locust tissue culture needs the aseptic condition that is used for medium preparation, sterilization, inoculation, cultivation and is used for scalable temperature and the full-automatic greenhouse of relative moisture and the operating technology of specialty of acclimatization and transplants; Have high input, survival rate is low, production cost is high, has restricted the popularization of forest improved seeds; Grafting method need be carried and cultivate rootstock seedling the previous year, and growing-seedling period is long, and the nursery stock of cultivating causes windbreak and form not straight because the growth rate difference of stock and scion forms " little pedopathy " easily after the transplanting.
Research to the cuttage of tetraploid locust is more; For example publication number is that the application for a patent for invention of CN 101637084A discloses a kind of tetraploid locust own-rooted tree that obtains through the cuttage approach; Adopt the root garden with own-rooted tree foundation;, be used plant growth regulator and realize the vegetative production method of tetraploid locust scale as propagating materials through the improved seeds root bar gathered.The method is grown seedlings cuttage and is combined with root segment, improved reproductive efficiency to a certain extent, but this inventive method utilizes cuttage acquisition own-rooted tree to adopt the root garden; Taking root segment is the cuttage material and since tetraploid locust a little less than the root ability, it is limited that can adopt root every year; The yield of cuttage material root segment is low; Particularly take the root segment material and have a strong impact on the growth of adopting the root maternal plant, even cause adopting the death of root maternal plant, and time-consuming; Seedling cost is high, has still limited a large amount of breedings and the cultivation of tetraploid locust.
Cuttage root-taking is the conventional method for propagation that adopts, but some are difficult to cuttage root-taking one-tenth plant alive except handling with growth hormone and some drugs, also usually adopts yellow to handle and inserts fringe, carries out plant propagation.Yellow is handled, and is a kind of measure that promotes cuttage rooting, and this processings is wrapped up in cover with the base portion of branch, cuts cuttage after covering the light certain hour, make most originally not the kinds of easy-to-use cottage propagation can both take root into alive after treatment.Branch is after yellow is handled, and chlorophyll disappears, organizes that yellow, cortex thicken, parenchyma cell increases, the differentiation of favourable original hase and taking root, and its reason is that the yellow position has increased Endogenous IAA, has reduced the material that is considered to suppress rooting effect and has produced.
The patent No. be ZL 200410009201.4 disclosure of the Invention a kind of Juglans trees softwood cutting propagation technology, graft technology and cuttage technique are organically combined, through multiple children technological with the scion grafting on stock, cultivate into grafting; Bury then to do and handle, grafting is covered sapin bury, promote the yellow of spray base portion with yellow; Again the spray base portion of yellow is carried out in growth regulator after speed dips in processing, cuttage in slotting machine, through the greenhouse transplant and field-transplanting after cuttage seeding is moved into the land for growing field crops, realized the breeding of Juglans trees.But this propagation method need combine grafting and cuttage technique, and propagation method is numerous and diverse, and seedling raise period is long; Scion is connected on the stock need carry out the cultivation in 1 year; Still there is the long defective of growing-seedling period, also increased seedling cost, bury the yellow of branch section with sand and handle the vernalization compared with techniques; Bury and do yellow and handle that vernalization is irregular, bud ratio is lower, be difficult to concentrate cuttage.
Publication number is the method that the patent of invention of CN 101243752A discloses forcing germination epicormic branch cuttage of Catalpa bungei root segment; After the root segment lamination that will take is handled, put into the vernalization furrow and carry out the vernalization processing, treat that the root segment tiller bears true leaf; When reaching semi-lignified, with carrying out cuttage after the growth regulator processing.This invention is carried out common lamination processing with root segment, though kept moisture to a certain extent, ventilative not enough; The material of gathering goes mouldy easily, and in addition, root segment is not easy to obtain; The complicated operation of uprooting, time-consuming, root segment quantity is wanted much less than branch; It is serious to the stock damage to gather root segment, influences sustainable growing seedlings, even causes the death of stock.
Summary of the invention
The objective of the invention is to the low technical problem of cuttage survival rate that exists in the above prior art tetraploid locust nursery stock that a kind of tetraploid locust cuttage breeding method is provided and obtains by this method.The tetraploid locust of the inventive method breeding is inserted fringe cuttage root-taking rate height, inserts the fringe survival rate and significantly improves, and has solved the technical barrier of tetraploid locust cuttage breeding.
For realizing above-mentioned purpose, one aspect of the present invention provides a kind of tetraploid locust cuttage breeding method, comprises as follows step in sequence:
1) chooses and prunes hard lopwood bar
Choose annual healthy and strong branch annual 10 the end of month, and the side shoot that prunes away is cut into the branch section, obtains hard lopwood section;
2) winter, husky the Tibetan handled
At first, hard lopwood section is soaked in the plant growth regulator, carries out the plant growth regulator processing first time, then the branch section is placed in the Sha Zangchi preservation of surviving the winter;
3) vernalization is handled
In early March, second will pass through the hard lopwood section that winter, handled husky Tibetan and lie in the germination accelerating pond, and cover wet rough sand above that, carry out vernalization and handle, and a section vernalization grows spray;
4) cuttage and culture of rootage
At first, the germination epicormic branch base portion dipped in rapidly gets plant growth regulator, carry out second time plant growth regulator and handle, will dip in then get growth regulator epicormic branch cuttage in cutting medium, carry out culture of rootage.
Wherein, the diameter of hard lopwood section is 10-20mm described in the step 1), and the branch segment length is 10-30cm.
Particularly, hard lopwood section diameter >=15mm, it is 10-20cm that the branch shed repair is cut into length; Hard lopwood section diameter is 10-15mm, and it is 20-30cm that the branch shed repair is cut into length.
Especially, the lower end of hard lopwood section is trimmed to shape of a hoof scarf, and the upper end is a side grain, and upper and lower otch is apart from bud 0.5cm.
Wherein, step 2) plant growth regulator described in is selected one or more in indolebutyric acid, methyl, the root-inducing powder, is preferably indolebutyric acid.
Particularly, step 2) described in for the first time the plant growth regulator immersion treatment time be 3-5min, be preferably 5min; The concentration of plant growth regulator is 100-200mg/kg.
Wherein, step 2) temperature of preservation of surviving the winter in is 0-7 ℃, and relative moisture is 70%-80%.
Particularly, said winter huskyly hide the time of handling and be meant early March from 10 the end of month to the second in every year year.
Wherein, a branch section vertical pendulum is placed among the Sha Zangchi in the processing procedure of branch section sand in winter Tibetan, carries out said husky Tibetan processing.
Particularly, the upper end that will pass through the hard lopwood section of tetraploid locust that plant growth regulator for the first time handles up, the lower end down, vertically be put among the Sha Zangchi preservation of surviving the winter.
Wherein, described Sha Zang pond is that the bottom is covered with crushed stone and wet rough sand, and the top covers with wet rough sand, and the heatable adobe sleeping platform of plastic film is covered in the superiors.
Particularly, described crushed stone, wet rough sand adopt carbendazim solution to carry out sterilization.
Wherein, the ratio of water and the quality of carbendazim is 2000: 1 in the carbendazim solution; Carbendazim solution is 1 with the ratio of the quality of crushed stone: 9-11; Carbendazim solution is 1 with the ratio of the quality of wet rough sand: 9-11.
Wherein, the thickness of wet rough sand is 1-7cm described in the step 3), is preferably 2-4cm; The moisture content of wet rough sand is 70%-80%, particle diameter >=2mm; Yellow vernalization treatment temperature is 10-30 ℃, is preferably 12-30 ℃; Relative moisture is 70%-90%, is preferably 75%-80%.
Particularly, will pass through the hard lopwood section that winter, handled husky Tibetan and lie in the germination accelerating pond, adjacent two hard lopwood spacer segment 1-3cm behind the wet rough sand of covering, carry out described yellow vernalization and handle.
Wherein, the growth regulator in the step 4) is selected one or more in indolebutyric acid, methyl, the root-inducing powder, is preferably indolebutyric acid.
Particularly, the concentration of said growth regulator is 1500-2000mg/kg, is preferably 2000mg/kg; Dipping in the time of getting growth regulator is preferably 10-30s, is preferably 30s; The germination epicormic branch base portion dips in to be got the plant growth regulator height and is >=2cm.
Wherein, the length of said germination epicormic branch is 12-15cm, diameter >=5mm.
Particularly, prune away the blade that removes said germination epicormic branch lower end, keep the blade on top, the blade amount of removal accounts for 1/2 of the total leaf amount of spray.
Wherein, the cuttage root-taking cultivation temperature is 15-30 ℃ in the step 4), and relative moisture is 70%-90%.
Particularly, carry out described adjustment through adding a cover the sunshade net; Regulate the relative moisture that cuttage root-taking is cultivated through automatic sprayer.
Also be included in described cuttage and culture of rootage and handle after 15 days, adopt measure refining seedlings such as ventilating, reduce injection flow rate, enhancing intensity of illumination.
Particularly, the matrix of cutting bed is cow dung and the thin sand and soil mixture of becoming thoroughly decomposed, and the cow dung of having become thoroughly decomposed is 2: 1 with the ratio of the volume of thin sand and soil.
Wherein, the said cow dung of having become thoroughly decomposed, thin sand and soil are sterilized with carbendazim solution respectively, mix then, and carbendazim solution is 1: 10 with the ratio of the quality of the cow dung of having become thoroughly decomposed, thin sand and soil.
Particularly, the ratio of water and the quality of carbendazim is 2000: 1 in the said carbendazim solution; Granularity >=the 1mm of said thin sand and soil, moisture content are 70%-80%.
The present invention provides a kind of tetraploid locust nursery stock that obtains according to above-mentioned arbitrary method breeding on the other hand.
The hard branch of tetraploid locust of the present invention propagation method has following advantage:
1, propagation method employing plant growth regulator of the present invention is handled the hard lopwood section of tetraploid locust, and the husky processing of hiding that combines to survive the winter, and has improved the moisture content of branch section; Promoted conversion sugared, nitrogenous compound; Help eliminating the inhibiting substances of taking root in the segment body, improve respiratory intensity, improve the differentiation capability of cell; Promote the formation of callus, the branch section is to the utilization of absorption of water and idiotrophic when helping vernalization.
2, the present invention covers the hard lopwood section of locust tree in the sand; Branch Duan Sha buries vernalization and handles; The spray blade is light yellow, tissue and the yellow of spray base portion, cortex thicken, parenchyma cell increases, and is beneficial to the differentiation of root original hase and takes root, and has improved the tetraploid locust cuttage reproductive efficiency, take root number, the length of taking root, rooting rate and survival rate; Traditional vernalization quantity of doing the vernalization processing of burying improves more than 2 times, and reproduction coefficient improves more than 2 times.
3, propagation method of the present invention adopts the high concentration plant growth regulator to handle yellow spray base portion, has improved the quantity of taking root of spray, the length of taking root and rooting rate, and survival rate reaches more than 90.0%; Do not compare with carrying out the direct cuttage of growth regulator processing spray, the quantity of taking root of the present invention has improved more than 1.2 times, and the length of taking root has increased more than 61.9%, and rooting rate has improved more than 1.9 times.
4, the present invention adopts growth regulator to soak the branch section to handle the husky rooting efficiency of handling of hiding that combines to survive the winter and be far superior to common husky the Tibetan and handle; Compare rooting rate than common husky Tibetan processing and improved 43.1%; The quantity of taking root has improved 65%, and the length of taking root has increased by 49.5%.Rooting efficiency is better than traditional sand and hides the lamination processing method.
5, adopt sand to bury the processing of branch section in the cottage method of the present invention and carry out vernalization; Cover the length of taking root of husky germination epicormic branch cuttage, the quantity of taking root, rooting rate and be significantly higher than the not spray of yellow of base portion; Than base portion not the rooting rate handled of yellow epicormic branch cuttage improved 18.9%; The quantity of taking root has improved 59.7%, and the length of taking root has increased by 37.4%.
6, locust tree propagation method of the present invention helps inserting taking root and surviving of fringe, and the cuttage root-taking rate reaches 81.1-90.6%, and the number of taking root reaches the 7.0-9.9 bar, and the length of taking root reaches 10.3-13.6cm.Propagation method of the present invention has tangible promotion and raising effect to the cuttage radication capability that promotes tetraploid locust, and rooting efficiency is good, and seedling raise period is fast, and reproductive efficiency is high, and seedling quality is high, can realize the production of growing seedlings on a large scale.
7, propagation method of the present invention adopts husky Tibetan processing in winter to keep the moisture of branch section; Keep the sprouting performance of branch section, simultaneously, the maize straw bundle among the Sha Zangchi can be ventilated with the external world; Preventing in the Duan Shazang process that heating goes rotten, is a kind of simple, cheap, storage practice efficiently.
Description of drawings
Fig. 1 is that sand buries the vernalization of branch section and handles not yellow spray aspect graph of base portion yellow spray and base portion.
The specific embodiment mode
Further describe the present invention below in conjunction with specific embodiment, advantage of the present invention and characteristics will be more clear along with description.But these embodiment only are exemplary, scope of the present invention are not constituted any restriction.It will be understood by those skilled in the art that and down can make amendment with form or replace without departing from the spirit and scope of the present invention, but these modifications and replacing all fall in protection scope of the present invention the details of technical scheme of the present invention.
The nursery, Lv Cun forest farm, Luoning County, Henan Province that experimental field is selected in of the present invention, be located in east longitude 111 ° 40 ', 34 ° 23 of north latitude ', belong to the continental monsoon climate in warm temperate zone, 13.7 ℃ of average temperatures of the whole year, 42.1 ℃ of the maximum air temperatures, the extreme minimum air temperature-21.3 ℃; Mean annual precipitation 606mm focused mostly in 6-8 month; Year evaporation discharge 1562.8mm, 216 days frost-free seasons.Climatic characteristic is that spring drought is windy, and summer heat is rainy, and refreshing sunshine in autumn is long, and long cold snow short of rain of winter makes a clear distinction between the four seasons, rain same season of heat.This nursery physical features is smooth, and soil is cinnamon soil, soil thickness 63cm, and permeability is good, middle fertility, the content of organic matter 0.85%, pH value 7.2, it is convenient to irrigate.
Test ground temperature canopy is divided into vernalization temperature canopy and cuttage temperature canopy.Vernalization temperature canopy is the cement skeleton, and one-way slope is to the south, builds germination accelerating pond in the canopy, lays automatic sprayer; Cuttage temperature canopy is a mao bamboon skeleton hut, lays automatic sprayer in the canopy, and the spray cooling facility is set up at the outer top of canopy.
The hard branch of the tetraploid locust that the present invention uses picks up from the tetraploid locust cutting orchard in above-mentioned nursery, is the annual stalwartness of tetraploid locust, no damage by disease and insect branch, and the diameter of branch is 10-20mm.
The wet rough sand of sterilization: the moisture content of wet rough sand is 70%-80%, particle diameter >=2mm; Wet rough sand adopts 2000 times of carbendazim (wherein; The ratio of water and carbendazim quality is 2000: 1) after solution evenly sprays; Stir, 2000 times of carbendazim solutions are 1 with the ratio of the quality of wet rough sand: 9-11, in case the husky branch section of hiding of various infection process causes living mycocriny mashed.
Sterilization crushed stone: adopt 2000 times of carbendazim (wherein; The ratio of water and carbendazim quality is 2000: 1) after solution evenly sprays; Stir, 2000 times of carbendazim solutions are 1 with the ratio of the quality of crushed stone: 9-11, in case the husky branch section of hiding of various infection process causes living mycocriny mashed.
Sterilization maize straw: adopt 2000 times of (ratio of water and carbendazim quality is 2000: 1) carbendazim evenly to spray the maize straw surface treatment, till the maize straw surface evenly covers carbendazim solution.
No. 1, the root-inducing powder (ABT) of the adoptable homemade indolebutyric acid of plant growth regulating substance (IBA), methyl (NAA) and the Chinese Academy of Forestry.
Embodiment 1
One, the collection of the hard branch of locust tree and growth regulator processing for the first time
1) 10 the end of month then, treat that tetraploid locust stops growing, falls leaves after, the annual stalwartness of clip, no damage by disease and insect branch; The side shoot of branch is removed in pruning, and the branch diameter is 10-20mm, wherein; With branch section diameter >=15mm be trimmed to 10-20cm branch section, branch section diameter is trimmed to 20-30cm branch section 10-15mm's, and the lower end of branch section is trimmed to shape of a hoof scarf; The upper end is a side grain, and upper and lower otch is apart from bud 0.5cm, and the diameter of branch section is 10-20mm;
2) per 30 of slotting fringe after will pruning is tied up into a bundle, forms branch section bundle, and wherein, the lower end of branch section separate with the upper end is strict in the branch section bundle, and promptly the lower end of all sections is positioned at an end of branch section bundle, and the upper end of all sections is positioned at a branch section other end of tying;
3) the branch section is tied be soaked in fully in growth regulator indolebutyric acid (IBA) solution, carry out first time growth regulator and handle, take out after the branch section is soaked 5min, the concentration of growth regulating agent solution is 200mg/kg.
Two, winter, sand was hidden
1, prepare Sha Zangchi:
Outside greenhouse experimental field, select the leeward place that faces south, the hole of cutting the earth is as husky Zang Chi, the dark 1m of Sha Zang Chi Chi wherein, area decide according to branch hop count amount, and the orlop shop thickness of Sha Zangchi is the crushed stone of sterilizing through carbendazim of 5-10cm; On metalling, repave a layer thickness then and be the wet rough sand of the sterilization through the carbendazim processing of 10cm, subsequent use;
2, the husky Tibetan handled:
To pass through branch section that growth regulator for the first time handles puts into Sha Zangchi rapidly and carries out sand and hide processing; Wherein branch section upper end up, the lower end vertically be put in down Sha Zangchi wet rough sand above; On four angles of Sha Zangchi with the vertical respectively maize straw of placing a branch of bacterium of going out of the central authorities of Sha Zangchi; The branch section of burying can be ventilated with the external world, prevent that the heating of branch section from causing mildew and rot;
The wet rough sand of sterilization that covering one deck is handled through carbendazim on the branch section, compacting is patted with spade in wet rough sand surface, does not interspace; The thickness of wet rough sand layer is 20cm; Then at the superiors' covered with plastic film of Sha Zangchi, with heat and moisture preserving, wherein; The temperature of Sha Zangchi remains 0-7 ℃, and relative moisture is 70%-80%.
Three, vernalization is handled
1, prepares germination accelerating pond
Dig the germination accelerating pond of wide 2m, dark 0.4m on the ground in experimental field vernalization temperature canopy, the length of germination accelerating pond is decided according to branch hop count amount;
Automatic sprayer is laid on germination accelerating pond top, and the crushed stone of the thick carbendazim sterilization of 5-10cm is spread in the bottom of germination accelerating pond, the wet rough sand of the carbendazim sterilization that shop 10cm is thick on crushed stone;
2, branch section sterilization treatment
At in early March, second; Take out the husky hard lopwood section of handling of hiding in winter; Evenly spray the branch section with 2000 times of carbendazim (water and carbendazim mass ratio are 2000: 1) solution, carry out branch section sterilization treatment, repeat to spray till the branch section surface evenly covers carbendazim solution;
3, sand buries the vernalization of branch section
The branch section of at first will sterilizing horizontal positioned evenly is placed on the wet rough sand of germination accelerating pond, interval 1-3cm between each section; Then on the branch section, cover the thick wet rough sand of carbendazim sterilization of 2cm, water spray keeps the relative moisture in the germination accelerating pond in good time, carries out vernalization and handles, and wherein, in the vernalization processing procedure, the temperature of vernalization temperature canopy is between 12-30 ℃, and relative moisture is 75%-80%.
Four, cuttage is handled
1, prepares cutting medium
Use 2000 times of carbendazim (water and carbendazim mass ratio are 2000: 1) solution evenly to spray respectively the cow dung of having become thoroughly decomposed, thin sand and soil; Stir; Hide the branch section and cause living mycocriny mashed in case various infection processes are husky, wherein, 2000 times of carbendazim solutions are 1: 10 with the ratio of the quality of the cow dung of having become thoroughly decomposed, thin sand and soil; Thin sand and soil granularity >=1mm, moisture content are 70%-80%;
The cow dung of having become thoroughly decomposed and the thin sand and soil of carbendazim sterilization are mixed, as cutting medium, in the nutrient cup of packing into; Nutrient cup closely is placed in the cuttage temperature canopy experimental field, and is subsequent use, wherein; The specification of nutrient cup is 8cm * 8cm, and the cow dung of having become thoroughly decomposed is 2: 1 with the ratio of the volume of thin sand and soil.
2, spray is handled
The early April in 1 year; The spray of treating the hard lopwood section germinating in the germination accelerating pond grows to 12-15cm, during diameter >=5mm, spray band bastem is cut or breaks off with the fingers and thumb down from branch section attachment region; Prune away the blade that removes the spray lower end; Keep the blade on top, the blade amount of removal accounts for 1/2 of the total leaf amount of spray, and it is subsequent use to obtain germination epicormic branch;
The germination epicormic branch base portion dipped in rapidly get plant growth regulator indolebutyric acid (IBA), wherein the concentration of IBA solution-treated is 2000mg/kg, and dipping in the time of getting is 30s, and the germination epicormic branch base portion dips in the degree of depth 2cm that gets.
3, cuttage is handled
In nutrient cup, the slotting fringe of 1 base portion yellow of cuttage in each nutrient cup is inserted fringe and in nutrient cup, is carried out culture of rootage with the shoot cutting fringe cuttage after handling;
On cuttage temperature canopy, cover the sunshade net after the cuttage and shelter from heat or light, utilize the automatic sprayer of cuttage temperature canopy to carry out atomized water spray simultaneously, to keep the blade face moistening; (spray once about 10 minutes in diligent spray at noon; About each spray 10s), morning, afternoon spray less, stop spray evening; Temperature in the cuttage temperature canopy remains in 15-30 ℃, and relative moisture is 70%-90%;
After the cuttage 11 days, reduce the water spray number of times gradually, cuttage is after 15 days, mainly is to adopt to ventilate, reduce injection flow rate, strengthen measures such as intensity of illumination and begin to refine seedling, measures the length of taking root, the quantity of taking root of inserting fringe simultaneously, calculates rooting rate, and testing result is seen table 1.
Embodiment 2
Except the husky branch section vernalization step of burying in the vernalization processing procedure covers the thick wet rough sand of 4cm on the branch section, all the other are identical with embodiment 1.
After the cuttage 15 days, mainly be to adopt measures such as ventilating, reduce injection flow rate, enhancing intensity of illumination to begin to refine seedling, measure the length of taking root, the quantity of taking root of inserting fringe simultaneously, calculate rooting rate, testing result is seen table 1.
Embodiment 3
Except adopting concentration in the growth regulator processing procedure first time is the IBA solution soaking branch section of 100mg/kg, and soak time is 3min; The husky branch section vernalization step of burying covers the thick wet rough sand of 4cm in the vernalization processing procedure on the branch section; Processing in the cuttage processing procedure is inserted the IBA solution-treated that adopts 1500mg/kg in the fringe step and is inserted the fringe base portion, and dipping in the time of getting is outside the 20s, and all the other are identical with embodiment 1.
After the cuttage 15 days, mainly be to adopt measures such as ventilating, reduce injection flow rate, enhancing intensity of illumination to begin to refine seedling, measure the length of taking root, the quantity of taking root of inserting fringe simultaneously, calculate rooting rate, testing result is seen table 1.
Embodiment 4
Except adopting concentration in the growth regulator processing procedure first time is the NAA solution soaking branch section of 200mg/kg, and soak time is 3min; The husky branch section vernalization step of burying covers the thick wet rough sand of 2cm in the vernalization processing procedure on the branch section, and the processing in the cuttage processing procedure is inserted the NAA solution-treated that adopts 2000mg/kg in the fringe step and inserted outside the fringe base portion, and all the other are identical with embodiment 1.
After the cuttage 15 days, mainly be to adopt measures such as ventilating, reduce injection flow rate, enhancing intensity of illumination to begin to refine seedling, measure the length of taking root, the quantity of taking root of inserting fringe simultaneously, calculate rooting rate, testing result is seen table 1.
Embodiment 5
Except adopting concentration in the growth regulator processing procedure first time is the NAA solution soaking branch section of 100mg/kg; The husky branch section vernalization step of burying covers the thick wet rough sand of 2cm in the vernalization processing procedure on the branch section; Processing in the cuttage processing procedure is inserted the NAA solution-treated that adopts 1500mg/kg in the fringe step and is inserted outside the fringe base portion, and all the other are identical with embodiment 1.
After the cuttage 15 days, mainly be to adopt measures such as ventilating, reduce injection flow rate, enhancing intensity of illumination to begin to refine seedling, measure the length of taking root, the quantity of taking root of inserting fringe simultaneously, calculate rooting rate, testing result is seen table 1.
Embodiment 6
Except adopting concentration in the growth regulator processing procedure first time is the ABT solution soaking branch section of 200mg/kg, and soak time is 3min; The husky branch section vernalization step of burying covers the thick wet rough sand of 3cm in the vernalization processing procedure on the branch section; Processing in the cuttage processing procedure is inserted the ABT solution-treated that adopts 2000mg/kg in the fringe step and is inserted outside the fringe base portion, and all the other are identical with embodiment 1.
After the cuttage 15 days, mainly be to adopt measures such as ventilating, reduce injection flow rate, enhancing intensity of illumination to begin to refine seedling, measure the length of taking root, the quantity of taking root of inserting fringe simultaneously, calculate rooting rate, testing result is seen table 1.
Embodiment 7
Except adopting concentration in the first time growth regulator processing procedure is that the ABT of 100mg/kg soaks the branch section; The husky branch section vernalization step of burying covers the thick wet rough sand of 2cm in the vernalization processing procedure on the branch section; Processing in the cuttage processing procedure is inserted the ABT solution-treated that adopts 1500mg/kg in the fringe step and is inserted outside the fringe base portion, and all the other are identical with embodiment 1.
After the cuttage 15 days, mainly be to adopt measures such as ventilating, reduce injection flow rate, enhancing intensity of illumination to begin to refine seedling, measure the length of taking root, the quantity of taking root of inserting fringe simultaneously, calculate rooting rate, testing result is seen table 1.
Reference examples 1
Do not cover wet rough sand except sand in the vernalization processing procedure buries on the branch section vernalization step branch section, sand buries the vernalization of branch section and obtains base portion not outside the spray of yellow, and all the other are identical with embodiment 1.
After the cuttage 15 days, mainly be to adopt measures such as ventilating, reduce injection flow rate, enhancing intensity of illumination to begin to refine seedling, measure the length of taking root, the quantity of taking root of inserting fringe simultaneously, calculate rooting rate, testing result is seen table 1.
Reference examples 2
Before handle the sand Tibetan, do not adopt growth regulator to soak the processing of branch section, other is all identical with embodiment 1.
After the cuttage 15 days, mainly be to adopt measures such as ventilating, reduce injection flow rate, enhancing intensity of illumination to begin to refine seedling, measure the length of taking root, the quantity of taking root of inserting fringe simultaneously, calculate rooting rate, testing result is seen table 1.
Reference examples 3
Germination epicormic branch does not dip in and gets the growth regulator in the cuttage processing procedure, and other is all identical with embodiment 1.
After the cuttage 20 days, mainly be to adopt measures such as ventilating, reduce injection flow rate, enhancing intensity of illumination to begin to refine seedling, measure the length of taking root, the quantity of taking root of inserting fringe simultaneously, calculate rooting rate, testing result is seen table 1.
Table 1 is inserted the multiple comparison sheet of cuttage root-taking effect of fringe
| Rooting rate (%) | Take root and count (bar) | The length of taking root (cm) | |
| Embodiment 1 | 89.4a | 9.2a | 13.6a |
| Embodiment 2 | 88.5a | 8.9a | 13.0a |
| Embodiment 3 | 82.1ab | 7.5b | 11.3b |
| Embodiment 4 | 85.6a | 8.3b | 11.2b |
| Embodiment 5 | 81.1ab | 7.0b | 10.3bc |
| Embodiment 6 | 90.6a | 9.9a | 13.3a |
| Embodiment 7 | 85.1a | 8.0b | 11.9ab |
| Reference examples 1 | 76.2bc | 6.2c | 9.9c |
| Reference examples 2 | 63.3c | 6.0c | 9.1c |
| Reference examples 3 | 31.5d | 4.5d | 8.4c |
Annotate: it is not remarkable that the same column letter is all difference mutually, is not all significant difference (P＜0.05)
Testing result shows:
Cuttage root-taking rate, the quantity of taking root and the length of taking root of the hard branch of tetraploid locust of the present invention yellow cottage method significantly improve, and explain that cottage method of the present invention more helps inserting taking root and surviving of fringe.The cuttage root-taking rate of the inventive method reaches 81.1-90.6%; The number of taking root reaches the 7.0-9.9 bar; The length of taking root reaches 10.3-13.6cm, compares with reference examples 1 (being not yellow epicormic branch cuttage of base portion), and rooting rate has improved 18.9%; The quantity of taking root has improved 59.7%, and the length of taking root has increased by 37.4%; Compare with reference examples 2 (the branch section of promptly not carrying out the growth regulator processing is directly carried out the processing of sand Tibetan), rooting rate has improved 43.1%, and the quantity of taking root has improved 65.0%, and the length of taking root has increased by 49.5%; Compare with reference examples 3 (being that germination epicormic branch does not carry out the growth regulator processing), rooting rate has improved more than 1.9 times, and the quantity of taking root has improved more than 1.2 times, and the length of taking root has increased more than 61.9%.
Claims (4)
1. tetraploid locust cuttage breeding method comprises as follows step in sequence:
1) chooses and prunes hard branch
Choose annual healthy and strong branch annual 10 the end of month, and the side shoot that prunes away is cut into the branch section, obtains hard lopwood section;
2) winter, husky the Tibetan handled
At first; Hard lopwood section is soaked in the plant growth regulator, carries out the plant growth regulator processing first time, then the branch section is placed in the Sha Zangchi; The preservation of surviving the winter; Wherein, it is in the indolebutyric acid, methyl, root-inducing powder of 100-200mg/kg one or more that plant growth regulator is selected concentration, and the plant growth regulator processing time is 3-5min for the first time;
3) vernalization is handled
In early March, second will pass through the hard lopwood section that winter, handled husky Tibetan and lie in the germination accelerating pond, and cover wet rough sand above that, carry out vernalization and handle, and a section vernalization grows spray;
4) cuttage and culture of rootage
At first; The germination epicormic branch base portion dipped in gets plant growth regulator, carry out second time plant growth regulator and handle, will dip in then get growth regulator epicormic branch cuttage in cutting medium; Carry out culture of rootage; Wherein, it is in the indolebutyric acid, methyl, root-inducing powder of 1500-2000mg/kg one or more that plant growth regulator is selected concentration, and plant growth regulator time of handling is 10-30s for the second time.
2. the method for claim 1 is characterized in that step 2) described in the temperature of the preservation of surviving the winter be 0-7 ℃, relative moisture is 70%-80%.
3. according to claim 1 or claim 2 method, the thickness that it is characterized in that the wet rough sand described in the step 3) is 1-7cm.
4. according to claim 1 or claim 2 method is characterized in that regulating temperature in the step 3) is 10-30 ℃, and adjusting relative moisture is 70%-90%; Regulating temperature in the step 4) is 15-30 ℃, and adjusting relative moisture is 70%-90%.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101637084A (en) * | 2009-09-03 | 2010-02-03 | 西北农林科技大学 | Method for breeding tetraploid robinia pseudoacacia seedlings by combining hardwood cutting and root seedling raising |
-
2010
- 2010-04-26 CN CN2010101607954A patent/CN101816251B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101637084A (en) * | 2009-09-03 | 2010-02-03 | 西北农林科技大学 | Method for breeding tetraploid robinia pseudoacacia seedlings by combining hardwood cutting and root seedling raising |
Non-Patent Citations (2)
| Title |
|---|
| 杨兴芳 等.四倍体刺槐硬枝扦插技术研究.《山东林业科技》.2007,(第2期),全文. * |
| 郝朝晖等.四倍体刺槐的繁育与推广.《林业科技开发》.2005,第19卷(第3期),全文. * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106577227A (en) * | 2016-12-02 | 2017-04-26 | 四川中科水务科技有限公司 | Quick cutting propagation method of lythrum salicaria |
| CN108055932A (en) * | 2017-12-19 | 2018-05-22 | 安徽泓森高科林业股份有限公司 | Locust tree radicula section seedling-raising technique |
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