CN101760469A - 转化破囊壶菌目微生物的产物和方法 - Google Patents

转化破囊壶菌目微生物的产物和方法 Download PDF

Info

Publication number
CN101760469A
CN101760469A CN200910141898A CN200910141898A CN101760469A CN 101760469 A CN101760469 A CN 101760469A CN 200910141898 A CN200910141898 A CN 200910141898A CN 200910141898 A CN200910141898 A CN 200910141898A CN 101760469 A CN101760469 A CN 101760469A
Authority
CN
China
Prior art keywords
seq
nucleic acid
sequence
ala
val
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN200910141898A
Other languages
English (en)
Chinese (zh)
Inventor
保罗·G·罗斯勒
T·戴夫·马修斯
汤姆·M·拉姆西尔
詹姆斯·G·梅茨
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DSM IP Assets BV
Original Assignee
Martek Biosciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Martek Biosciences Corp filed Critical Martek Biosciences Corp
Publication of CN101760469A publication Critical patent/CN101760469A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y202/00—Transferases transferring aldehyde or ketonic groups (2.2)
    • C12Y202/01—Transketolases and transaldolases (2.2.1)
    • C12Y202/01006—Acetolactate synthase (2.2.1.6)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Mushroom Cultivation (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
CN200910141898A 2001-04-16 2002-04-16 转化破囊壶菌目微生物的产物和方法 Pending CN101760469A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US28411601P 2001-04-16 2001-04-16
US60/284,116 2001-04-16

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN028120590A Division CN1556850B (zh) 2001-04-16 2002-04-16 转化破囊壶菌目微生物的产物和方法

Publications (1)

Publication Number Publication Date
CN101760469A true CN101760469A (zh) 2010-06-30

Family

ID=23088908

Family Applications (3)

Application Number Title Priority Date Filing Date
CN200910141898A Pending CN101760469A (zh) 2001-04-16 2002-04-16 转化破囊壶菌目微生物的产物和方法
CN028120590A Expired - Fee Related CN1556850B (zh) 2001-04-16 2002-04-16 转化破囊壶菌目微生物的产物和方法
CNB2006100842683A Expired - Fee Related CN100513564C (zh) 2001-04-16 2002-04-16 转化破囊壶菌目微生物的产物和方法

Family Applications After (2)

Application Number Title Priority Date Filing Date
CN028120590A Expired - Fee Related CN1556850B (zh) 2001-04-16 2002-04-16 转化破囊壶菌目微生物的产物和方法
CNB2006100842683A Expired - Fee Related CN100513564C (zh) 2001-04-16 2002-04-16 转化破囊壶菌目微生物的产物和方法

Country Status (10)

Country Link
US (5) US7001772B2 (cg-RX-API-DMAC10.html)
EP (3) EP2325319B1 (cg-RX-API-DMAC10.html)
JP (4) JP4536324B2 (cg-RX-API-DMAC10.html)
CN (3) CN101760469A (cg-RX-API-DMAC10.html)
AT (1) ATE533844T1 (cg-RX-API-DMAC10.html)
AU (1) AU2002250592C1 (cg-RX-API-DMAC10.html)
CA (1) CA2443908C (cg-RX-API-DMAC10.html)
ES (3) ES2698473T3 (cg-RX-API-DMAC10.html)
TW (3) TWI377253B (cg-RX-API-DMAC10.html)
WO (1) WO2002083869A2 (cg-RX-API-DMAC10.html)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109477079A (zh) * 2016-05-12 2019-03-15 帝斯曼知识产权资产管理有限公司 增加微藻中ω-3多不饱和脂肪酸产量的方法

Families Citing this family (79)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6566583B1 (en) * 1997-06-04 2003-05-20 Daniel Facciotti Schizochytrium PKS genes
US20070244192A1 (en) * 1999-01-14 2007-10-18 Martek Biosciences Corporation Plant seed oils containing polyunsaturated fatty acids
US7217856B2 (en) * 1999-01-14 2007-05-15 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
US7247461B2 (en) * 1999-01-14 2007-07-24 Martek Biosciences Corporation Nucleic acid molecule encoding ORFA of a PUFA polyketide synthase system and uses thereof
US7211418B2 (en) 1999-01-14 2007-05-01 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
US8003772B2 (en) 1999-01-14 2011-08-23 Martek Biosciences Corporation Chimeric PUFA polyketide synthase systems and uses thereof
TWI377253B (en) * 2001-04-16 2012-11-21 Martek Biosciences Corp Product and process for transformation of thraustochytriales microorganisms
US7211656B2 (en) † 2002-01-30 2007-05-01 Abbott Laboratories Desaturase genes, enzymes encoded thereby, and uses thereof
DK1572994T3 (da) * 2002-12-20 2007-05-29 Chromagenics Bv Midler og fremgangsmåder til fremstilling af en protein gennem chromatinåbnere, som kan göre chromatin mere tilgængeligt for transkriptionsfaktorer
US7208590B2 (en) * 2003-07-15 2007-04-24 Abbott Laboratories Genes involved in polyketide synthase pathways and uses thereof
ES2529572T3 (es) 2004-04-22 2015-02-23 Commonwealth Scientific And Industrial Research Organisation Síntesis de ácidos grasos poliinsaturados de cadena larga por células recombinantes
CA3056110C (en) 2004-04-22 2020-07-14 Surinder Pal Singh Synthesis of long-chain polyunsaturated fatty acids by recombinant cells
US8999667B2 (en) * 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20060195935A1 (en) 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
BRPI0517380A (pt) * 2004-11-08 2008-10-07 Chromagenics Bv molécula de dna, cassete de expressão, célula hospedeira, e, métodos para gerar uma célula hospedeira que expressa um polipeptìdeo de interesse e para produzir um polipeptìdeo de interesse
US20100136616A1 (en) * 2004-11-08 2010-06-03 Chromagenics B.V. Selection of Host Cells Expressing Protein at High Levels
NZ553700A (en) * 2004-11-08 2010-09-30 Chromagenics Bv Selection of host cells expressing protein at high levels using a selectable marker with a non-optimal start codon
JP4796786B2 (ja) 2005-04-28 2011-10-19 富士フイルム株式会社 ラビリンチュラ類を形質転換可能なベクター
JP4796787B2 (ja) * 2005-04-28 2011-10-19 富士フイルム株式会社 ラビリンチュラ類への遺伝子導入法
ES2909600T3 (es) 2005-06-07 2022-05-09 Dsm Nutritional Products Ag Microorganismos eucarióticos para producir lípidos y antioxidantes
CA2624661C (en) 2005-11-23 2015-06-30 E.I. Du Pont De Nemours And Company Delta-9 elongases and their use in making polyunsaturated fatty acids
EP1818411A1 (en) * 2006-02-13 2007-08-15 Lonza AG Process for the preparation of optically active chiral amines
WO2007106903A2 (en) 2006-03-15 2007-09-20 Martek Biosciences Corporation Polyunsaturated fatty acid production in heterologous organisms using pufa polyketide synthase systems
CA2649118A1 (en) * 2006-04-11 2007-10-25 Martek Biosciences Corporation Food products comprising long chain polyunsaturated fatty acids and methods for preparing the same
PL2034973T5 (pl) 2006-06-22 2023-02-20 Dsm Ip Assets B.V. Kapsułkowane kompozycje nietrwałych związków i sposoby ich wytwarzania
US9023616B2 (en) * 2006-08-01 2015-05-05 Dsm Nutritional Products Ag Oil producing microbes and method of modification thereof
EP2415360A3 (en) 2006-08-29 2012-02-29 Martek Biosciences Corporation Use of DPA(n-6) oils in infant formula
RU2009111266A (ru) 2006-08-29 2010-10-10 Коммонвелт Сайентифик энд Индастриал Рисерч Организейшн (AU) Синтез жирных кислот
KR100860111B1 (ko) * 2006-10-02 2008-09-25 인제대학교 산학협력단 신규 미생물 트라우스토카이트리움 에스피.케이제이에스-1, 바실러스 폴리퍼멘티쿠스 케이제이에스-2 및 이들과 바실러스 리케니포르미스를 포함하고 있는 사료첨가제 및 사료
UA102059C2 (uk) 2006-10-23 2013-06-10 Е.І. Дю Пон Де Немур Енд Компані Дельта-8 десатураза та її застосування для одержання поліненасичених жирних кислот
US7790156B2 (en) 2007-04-10 2010-09-07 E. I. Du Pont De Nemours And Company Δ-8 desaturases and their use in making polyunsaturated fatty acids
US7794701B2 (en) 2007-04-16 2010-09-14 E.I. Du Pont De Nemours And Company Δ-9 elongases and their use in making polyunsaturated fatty acids
US8957280B2 (en) 2007-05-03 2015-02-17 E. I. Du Pont De Nemours And Company Delta-5 desaturases and their use in making polyunsaturated fatty acids
US20090023808A1 (en) * 2007-06-29 2009-01-22 Martek Biosciences Corporation Production and Purification of Esters of Polyunsaturated Fatty Acids
EA019387B1 (ru) 2007-09-12 2014-03-31 Мартек Биосайнсиз Корпорейшн Способ получения биологического масла и его использование в способе получения биодизеля
US20110076379A1 (en) * 2008-06-13 2011-03-31 Means Michael M High omega saturated fat free meat products
DK2358882T3 (da) 2008-11-18 2017-11-06 Commw Scient Ind Res Org Enzymer og fremgangsmåder til fremstilling af omega-3 fedtsyrer
EP2358862B1 (en) 2008-12-18 2017-08-02 E. I. du Pont de Nemours and Company Reducing byproduction of malonates in a fermentation process
JP5415896B2 (ja) 2009-01-29 2014-02-12 Ｊｆｅスチール株式会社 インダイレクトスポット溶接方法
CN102648207A (zh) 2009-03-16 2012-08-22 马太克生物科学公司 在网粘菌门微生物中产生蛋白质
ES2717102T3 (es) 2009-03-19 2019-06-19 Dsm Ip Assets Bv Moléculas de ácido nucleico de ácido graso poliinsaturado sintasas, y polipéptidos, composiciones y métodos de preparación y usos de los mismos
US8207363B2 (en) 2009-03-19 2012-06-26 Martek Biosciences Corporation Thraustochytrids, fatty acid compositions, and methods of making and uses thereof
JP5931721B2 (ja) 2009-06-16 2016-06-08 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ アシルＣｏＡリゾリン脂質アシルトランスフェラーゼの発現による長鎖オメガ−３及びオメガ−６多価不飽和脂肪酸生合成の改良
EP2966169A1 (en) * 2009-09-24 2016-01-13 Kyushu University Method for transforming stramenopiles
EA030095B1 (ru) * 2009-12-28 2018-06-29 ДиЭсЭм АйПи АССЕТС Б.В. Получение белка гемагглютинин-нейраминидазы в микроводорослях
EP3213766A1 (en) 2009-12-28 2017-09-06 Merial Ltd. Recombinant ndv antigen and uses thereof
CA2785971C (en) * 2009-12-28 2019-02-12 Dsm Ip Assets B.V. Production of heterologous polypeptides in microalgae, microalgal extracellular bodies, compositions, and methods of making and uses thereof
CN102906270B (zh) * 2009-12-28 2016-06-22 Dsmip资产公司 在木糖上生长的重组破囊壶菌和其组合物、制备方法及用途
EP2519625B1 (en) * 2009-12-28 2018-09-26 DSM IP Assets B.V. Recombinant thraustochytrids that grow on sucrose, and compositions, methods of making, and uses thereof
CA2993690C (en) 2010-01-19 2022-03-22 Dsm Ip Assets B.V. Eicosapentaenoic acid-producing microorganisms, fatty acid compositions, and methods of making and uses thereof
WO2011112945A2 (en) 2010-03-12 2011-09-15 Merial Limited Foot and mouth disease virus recombinant vaccines and uses thereof
JP2013535988A (ja) 2010-08-26 2013-09-19 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 変異ｈｐｇｇモチーフおよびｈｄａｓｈモチーフδ５デサチュラーゼおよび多価不飽和脂肪酸の製造におけるそれらの使用
JP5963749B2 (ja) 2010-08-26 2016-08-03 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ 変異δ９エロンガーゼおよび多価不飽和脂肪酸の製造におけるそれらの使用
US9133463B2 (en) * 2011-03-07 2015-09-15 Dsm Nutritional Products Ag Engineering microorganisms
MX342216B (es) 2011-07-21 2016-09-20 Dsm Ip Assets Bv Composiciones de acidos grasos.
US8950312B2 (en) * 2011-08-17 2015-02-10 Lwrc International Llc Bolt carrier and bolt for gas operated firearms
US8844424B2 (en) * 2011-08-17 2014-09-30 Lwrc International Llc Bolt carrier and bolt for gas operated firearms
US8946460B2 (en) 2012-06-15 2015-02-03 Commonwealth Scientific And Industrial Research Organisation Process for producing polyunsaturated fatty acids in an esterified form
WO2013192002A1 (en) 2012-06-19 2013-12-27 E. I. Du Pont De Nemours And Company IMPROVED PRODUCTION OF POLYUNSATURATED FATTY ACIDS BY COEXPRESSION OF ACYL-CoA:LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASES AND PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASES
AU2014229307B2 (en) 2013-03-13 2017-08-31 Dsm Nutritional Products Ag Engineering microorganisms
WO2015196250A1 (en) 2014-06-27 2015-12-30 Commonwealth Scientific And Industrial Research Organisation Lipid comprising docosapentaenoic acid
KR102386838B1 (ko) 2013-12-18 2022-04-15 커먼웰쓰 사이언티픽 앤 인더스트리알 리서치 오거니제이션 장쇄 다중불포화 지방산을 포함하는 지질
CN105567715B (zh) * 2014-10-17 2021-06-29 丰益(上海)生物技术研发中心有限公司 裂殖壶菌α-微管蛋白相关序列及其应用
EP3265567B1 (en) 2015-03-02 2020-07-29 Conagen Inc. Regulatory elements from labyrinthulomycetes microorganisms
AR104042A1 (es) 2015-03-26 2017-06-21 Mara Renewables Corp Producción de alta densidad de biomasa y aceite utilizando glicerol en bruto
EP3302032B1 (en) 2015-06-06 2021-12-15 DSM IP Assets B.V. Production of polyunsaturated fatty acids (pufas) using a novel modular docosahexaenoic acid (dha) synthase
US10542961B2 (en) 2015-06-15 2020-01-28 The Research Foundation For The State University Of New York System and method for infrasonic cardiac monitoring
CN122256151A (zh) * 2015-07-13 2026-06-23 玛拉可再生能源公司 增强木糖的微藻代谢
IL241462A0 (en) 2015-09-10 2015-11-30 Yeda Res & Dev Heterologous engineering of betalain pigments in plants
AU2017235543B2 (en) 2016-03-16 2020-05-14 Conagen, Inc. Production of proteins in labyrinthulomycetes
US10851395B2 (en) 2016-06-10 2020-12-01 MARA Renewables Corporation Method of making lipids with improved cold flow properties
WO2018013821A1 (en) 2016-07-13 2018-01-18 Dsm Ip Assets B.V. A crispr-cas system for an algal host cell
IL247752A0 (en) 2016-09-11 2016-11-30 Yeda Res & Dev Compositions and methods for modulating gene expression for site-directed mutagenesis
US10633454B2 (en) 2016-11-01 2020-04-28 Conagen Inc. Expression of modified glycoproteins and glycopeptides
WO2019078359A1 (ja) 2017-10-19 2019-04-25 味の素株式会社 ステロールの製造法
WO2019173226A1 (en) * 2018-03-05 2019-09-12 Synthetic Genomics, Inc. Organisms and methods for producing glycomolecules with low sulfation
EP3788164A4 (en) * 2018-05-01 2022-01-26 Conagen Inc. RECOMBINANT ORGANISMS AND METHODS FOR PRODUCTION OF HIGH GLYCAN OCCUPANCY GLYCOMOLECULES
WO2020032261A1 (ja) * 2018-08-10 2020-02-13 協和発酵バイオ株式会社 エイコサペンタエン酸を生産する微生物及びエイコサペンタエン酸の製造法
CN109777815B (zh) * 2019-03-28 2021-10-29 昆明理工大学 HMG-CoA合成酶基因RKHMGCS及其应用

Family Cites Families (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BE545283A (cg-RX-API-DMAC10.html)
LU35839A1 (cg-RX-API-DMAC10.html)
US3223456A (en) * 1963-11-14 1965-12-14 Siemens Ag Conveying apparatus for fine-granular material
US4381897A (en) * 1980-10-06 1983-05-03 Krupp Polysius Ag Installation for transporting fine-grained material
US4626505A (en) * 1984-02-24 1986-12-02 E. I. Du Pont De Nemours And Company Selectable markers for yeast transformation
US5605011A (en) * 1986-08-26 1997-02-25 E. I. Du Pont De Nemours And Company Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase
IL83348A (en) * 1986-08-26 1995-12-08 Du Pont Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase
US5130242A (en) * 1988-09-07 1992-07-14 Phycotech, Inc. Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids
US5340742A (en) 1988-09-07 1994-08-23 Omegatech Inc. Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids
US5426040A (en) 1989-08-17 1995-06-20 Northeastern University Methods for producing improved strains of seaweed by fusion of spore-protoplasts, and resultant seaweeds and phycocolloids
IL100908A0 (en) 1991-02-22 1992-11-15 Salk Inst Biotech Ind Invertase genes and their use
US5804408A (en) * 1991-03-13 1998-09-08 Yoshihide Hagiwara Expression of human SOD in blue green algae
US5661017A (en) * 1993-09-14 1997-08-26 Dunahay; Terri Goodman Method to transform algae, materials therefor, and products produced thereby
IL113375A (en) * 1995-04-13 1997-09-30 Fortress U & T Ltd Internationally regulated system for one to one cryptographic communications with national sovereignty without key escrow
DK0821729T3 (da) * 1995-04-20 2007-02-05 Basf Ag Strukturbaserede, udformede herbicidresistente produkter
US5853973A (en) * 1995-04-20 1998-12-29 American Cyanamid Company Structure based designed herbicide resistant products
DE19529475A1 (de) 1995-08-11 1997-02-13 Marcus Weis Siebkonstruktion zum Abtrennen des Faseranteils aus der Shredderleichtfraktion
WO1997020854A1 (en) 1995-12-05 1997-06-12 University Of Massachusetts Recombinant gas vesicles and uses thereof
WO1997039106A1 (en) * 1996-04-12 1997-10-23 Martek Biosciences Corporation Methods and tools for transformation of eukaryotic algae
US6194167B1 (en) * 1997-02-18 2001-02-27 Washington State University Research Foundation ω-3 fatty acid desaturase
US5968809A (en) * 1997-04-11 1999-10-19 Abbot Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids
ES2125195B1 (es) 1997-04-18 1999-10-01 Antibioticos Sa Procedimiento de inactivacion de genes que codifican para enzimas del catabolismo del fenilacetato, plasmidos que intervienen y cepas transformadas con los mismos.
US8003772B2 (en) 1999-01-14 2011-08-23 Martek Biosciences Corporation Chimeric PUFA polyketide synthase systems and uses thereof
US7247461B2 (en) 1999-01-14 2007-07-24 Martek Biosciences Corporation Nucleic acid molecule encoding ORFA of a PUFA polyketide synthase system and uses thereof
FR2791699B1 (fr) * 1999-03-29 2004-10-29 Meristem Therapeutics Promoteurs chimeriques d'expression, cassettes d'expression, vecteurs, plantes et semences transgeniques les contenant, et methodes de leur obtention
KR20000075076A (ko) * 1999-05-28 2000-12-15 최태진 형질전환된 미세조류를 이용하여 외래 단백질을 생산하는 방법
US20060029604A1 (en) 2000-06-28 2006-02-09 Gerngross Tillman U Immunoglobulins comprising predominantly a GlcNAc2Man3GlcNAc2 glycoform
US7029872B2 (en) 2000-06-28 2006-04-18 Glycofi, Inc Methods for producing modified glycoproteins
US20060257399A1 (en) 2000-06-28 2006-11-16 Glycofi, Inc. Immunoglobulins comprising predominantly a Man5GIcNAc2 glycoform
US7625756B2 (en) 2000-06-28 2009-12-01 GycoFi, Inc. Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells
US7795002B2 (en) 2000-06-28 2010-09-14 Glycofi, Inc. Production of galactosylated glycoproteins in lower eukaryotes
DK1911837T3 (da) 2000-09-28 2011-08-29 Bioriginal Food & Science Corp FAD5-2 fedtsyredesaturasefamiliemedlem og anvendelser deraf
US6419425B1 (en) * 2001-02-28 2002-07-16 Neu Transf'air Granular material distributing apparatus comprising at least two transfer vessels that operate in alternation
TWI377253B (en) * 2001-04-16 2012-11-21 Martek Biosciences Corp Product and process for transformation of thraustochytriales microorganisms
US7332299B2 (en) 2003-02-20 2008-02-19 Glycofi, Inc. Endomannosidases in the modification of glycoproteins in eukaryotes
US7541038B2 (en) 2003-06-05 2009-06-02 Wyeth Holdings Corporation Fusogenic, self-propagating blebs as immunogenic compositions
JP4796786B2 (ja) 2005-04-28 2011-10-19 富士フイルム株式会社 ラビリンチュラ類を形質転換可能なベクター
JP4796787B2 (ja) 2005-04-28 2011-10-19 富士フイルム株式会社 ラビリンチュラ類への遺伝子導入法
PT103331A (pt) 2005-08-05 2007-02-28 Fundacao Da Faculdade De Cienc Expressão dum transportador activo de xilose em saccharomyces cerevisiae modificada geneticamente
WO2007106903A2 (en) 2006-03-15 2007-09-20 Martek Biosciences Corporation Polyunsaturated fatty acid production in heterologous organisms using pufa polyketide synthase systems
US9023616B2 (en) 2006-08-01 2015-05-05 Dsm Nutritional Products Ag Oil producing microbes and method of modification thereof
CA2615372A1 (en) 2007-07-13 2009-01-13 Marc-Andre D'aoust Influenza virus-like particles (vlps) comprising hemagglutinin
EA019387B1 (ru) 2007-09-12 2014-03-31 Мартек Биосайнсиз Корпорейшн Способ получения биологического масла и его использование в способе получения биодизеля
EP2090648A1 (en) 2008-02-12 2009-08-19 Institut Francais de Recherche pour l'Exploitation de la Mere(Ifremer) Production of glycosylated polypeptides in microalgae
MX338039B (es) 2008-04-09 2016-03-30 Solazyme Inc Modificacion quimica directa de biomasa bacteriana y aceites microbianos.
CN102906270B (zh) 2009-12-28 2016-06-22 Dsmip资产公司 在木糖上生长的重组破囊壶菌和其组合物、制备方法及用途
EP2519625B1 (en) 2009-12-28 2018-09-26 DSM IP Assets B.V. Recombinant thraustochytrids that grow on sucrose, and compositions, methods of making, and uses thereof
EA030095B1 (ru) 2009-12-28 2018-06-29 ДиЭсЭм АйПи АССЕТС Б.В. Получение белка гемагглютинин-нейраминидазы в микроводорослях
CA2785971C (en) 2009-12-28 2019-02-12 Dsm Ip Assets B.V. Production of heterologous polypeptides in microalgae, microalgal extracellular bodies, compositions, and methods of making and uses thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109477079A (zh) * 2016-05-12 2019-03-15 帝斯曼知识产权资产管理有限公司 增加微藻中ω-3多不饱和脂肪酸产量的方法

Also Published As

Publication number Publication date
US8206984B2 (en) 2012-06-26
ES2698473T3 (es) 2019-02-04
JP2010142240A (ja) 2010-07-01
JP4536324B2 (ja) 2010-09-01
WO2002083869A3 (en) 2004-02-26
ES2688953T3 (es) 2018-11-07
EP2325319A3 (en) 2016-04-13
EP1414941A4 (en) 2005-06-01
US20090192304A1 (en) 2009-07-30
AU2002250592B2 (en) 2007-09-20
EP1414941B1 (en) 2011-11-16
CA2443908C (en) 2016-01-12
WO2002083869A9 (en) 2004-05-21
ES2377557T3 (es) 2012-03-28
ATE533844T1 (de) 2011-12-15
TW201002822A (en) 2010-01-16
JP6211577B2 (ja) 2017-10-11
TWI377253B (en) 2012-11-21
EP2314706B1 (en) 2018-09-12
US20050112719A1 (en) 2005-05-26
TWI350854B (en) 2011-10-21
CN1861795A (zh) 2006-11-15
TWI324181B (en) 2010-05-01
US20130295607A1 (en) 2013-11-07
HK1094711A1 (zh) 2007-04-04
JP2013005817A (ja) 2013-01-10
EP2325319A2 (en) 2011-05-25
EP2325319B1 (en) 2018-08-01
WO2002083869A2 (en) 2002-10-24
JP2005503125A (ja) 2005-02-03
CN1556850A (zh) 2004-12-22
CN1556850B (zh) 2012-09-26
EP2314706A2 (en) 2011-04-27
CN100513564C (zh) 2009-07-15
AU2002250592C1 (en) 2008-04-03
CA2443908A1 (en) 2002-10-24
EP2314706A3 (en) 2016-07-27
HK1065819A1 (en) 2005-03-04
US8945875B2 (en) 2015-02-03
US7851191B2 (en) 2010-12-14
US7001772B2 (en) 2006-02-21
TW201127960A (en) 2011-08-16
US20030166207A1 (en) 2003-09-04
JP5248536B2 (ja) 2013-07-31
JP2016039831A (ja) 2016-03-24
EP1414941A2 (en) 2004-05-06
US20110086390A1 (en) 2011-04-14

Similar Documents

Publication Publication Date Title
CA2443908C (en) Product and process for transformation of thraustochytriales microorganisms
DK2670846T3 (en) METHODS FOR THE DEVELOPMENT OF TERPEN SYNTHASE VARIETIES
KR102622910B1 (ko) Pd-1 호밍 엔도뉴클레아제 변이체, 조성물 및 사용 방법
AU2016349822B2 (en) Algal mutants with increased lipid productivity
CA2834053C (en) Yeast strains engineered to produce ethanol from glycerol
KR20210149060A (ko) Tn7-유사 트랜스포존을 사용한 rna-유도된 dna 통합
BRPI0806354A2 (pt) plantas oleaginosas transgências, sementes, óleos, produtos alimentìcios ou análogos a alimento, produtos alimentìcios medicinais ou análogos alimentìcios medicinais, produtos farmacêuticos, bebidas fórmulas para bebês, suplementos nutricionais, rações para animais domésticos, alimentos para aquacultura, rações animais, produtos de sementes inteiras, produtos de óleos misturados, produtos, subprodutos e subprodutos parcialmente processados
US20020132349A1 (en) Double transposition methods for manipulating nucleic acids
KR20150014953A (ko) 케톨-산 리덕토아이소머라아제 효소 및 사용 방법
KR20140146616A (ko) 부타놀로겐용 배지의 아세테이트 보충물
KR20140099251A (ko) 아세트산 및 글리세롤로부터 에탄올을 생성하도록 합성된 이스트 스트레인
CN108026504B (zh) 脂质产率增加的微生物
CN114480474B (zh) 一种海洋微拟球藻转录激活CRISPRa系统的构建及其应用
AU2016249402B2 (en) Algal chloroplastic SRP54 mutants
CN111041038B (zh) 高效生物合成虾青素的集胞藻6803基因工程菌及构建方法及应用
KR102527339B1 (ko) 일산화탄소 탈수소효소 및 포름산 탈수소효소를 이용한 개미산의 제조 방법
KR102292446B1 (ko) 형질전환 생물체 선별용 마커 조성물, 형질전환 생물체 및 형질전환 방법
HK1145338A (en) Product and process for transformation of thraustochytriales microorganisms
CN111433220A (zh) 通过基因修饰含trp结构域的蛋白来提高藻类脂质生产力
KR20140043890A (ko) 조절된 유전자 발현 시스템 및 그의 작제물
CN112384619A (zh) 具有增加的脂质产生的微生物和组合物及其制备和使用方法
KR20160090906A (ko) 에레모테시움의 발효를 통한 리보플라빈의 더 높은 생성을 위한 베타-산화 경로의 효소를 엔코딩하는 유전자 및 지방산 수송체 유전자의 과-발현
CN110241099B (zh) 酿脓链球菌的CRISPR核酸酶SpCas9 的截短变异体及其应用
CN117693592A (zh) 用于治疗遗传性疾患的体内核酸酶介导的基因靶向的组合物和方法
CN115247186B (zh) 一种构建af双基因突变的动脉粥样硬化模型猪核移植供体细胞的基因编辑系统及其应用

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 1145338

Country of ref document: HK

ASS Succession or assignment of patent right

Owner name: DSM IP ASSET LLP

Free format text: FORMER OWNER: MARTEK BIOSCIENCES BOULDER CORP.

Effective date: 20121115

C41 Transfer of patent application or patent right or utility model
TA01 Transfer of patent application right

Effective date of registration: 20121115

Address after: Holland Heerlen

Applicant after: DSM IP Asset Company

Address before: American Maryland

Applicant before: Martek Biosciences Corp.

RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20100630