KR102292446B1 - 형질전환 생물체 선별용 마커 조성물, 형질전환 생물체 및 형질전환 방법 - Google Patents
형질전환 생물체 선별용 마커 조성물, 형질전환 생물체 및 형질전환 방법 Download PDFInfo
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Abstract
본 발명은 항생제 및 항생제 저항성 유전자 없이 형질 전환 및 목적 산물의 생산이 가능하도록 하는 형질전환 생물체 선별용 마커 조성물, 형질전환 생물체, 형질전환 방법 및 목적 산물의 생산 방법에 관한 것으로, 항생제 및 항생제 저항성 유전자 사용에 기인한 문제를 원천적으로 예방하고, 목적 산물을 높은 수율로 생산할 수 있는 형질전환 생물체 선별용 마커 조성물, 형질전환 생물체, 형질전환 방법 및 목적 산물의 생산 방법에 관한 것이다.
Description
본 발명은 형질전환 생물체 선별용 마커 조성물, 형질전환 생물체 및 형질전환 방법에 관한 것이다.
유용 물질들의 산업적인 생산은 최근에는 대부분 LMO를 이용하고 있다. 이 경우에 대사경로 및 목적산물 생합성 경로의 발현 강화를 위해서 관련 유전자들을 다양한 발현 벡터(플라스미드)들 이용해서 발현을 유도하게 된다. 이 경우에 벡터들은 항생제 저항성 유전자를 선택 마커로 갖고 있으며 배양 과정 동안 숙주 세포 안에서 플라스미드의 안정한 유지를 위해서 배양액에 항생제를 첨가하게 된다. 그러나 배양 공정에서 항생제를 사용하게 되면 고가의 항생제 사용으로 인한 생산비용 상승, 항생제 유출로 인한 환경오염 및 자연계에 항생제 저항성 돌연연이 생물체 발생위험, 최종 산물에 항생제가 잔류하여 추가 분리 정제 공정의 필요, 항생제 저항성 유전자 마커 함유 균주 사용 및 허가 취득의 어려움 등의 문제가 있다.
아울러 항생제 사용 배양의 경우에 배양시간이 길어지면 항생제의 분해 및 변형으로 인해서 항생제 저항성 유전자를 선택마커로 갖고 있는 플라스미드의 소실이 발생하고 배양 후반기의 생산성의 급격한 감소를 초래한다. 항생제의 분해 및 변형은 항생제 마커 유전자에서 발현된 효소에 의한 것과 항생제의 불안정성에 의한 자연발생적인 것으로 2차 산물 생산 등의 장시간 발효를 요하는 배양공정에서 심각한 문제를 유발한다.
이런 문제를 해결하기 위해서 목적산물 생산에 필요한 외래 유전자들을 숙주 생물체의 염색체에 삽입하는 방법도 있지만, 복수의 유전자 카피를 갖는 플라스미드 도입에 비해 유전자량의 감소에 따른 단백질 발현량 감소, 염색체에의 다수의 유전자 도입 및 발현의 어려움 등의 문제가 있다.
상기의 이유들로 항생제 마커프리(antibiotic marker-free) 생물체를 개발하는 것이 최근 생물공정 산업계에서는 이슈가 되고 있다. 그러나 현재까지는 산업계에서 안정적으로 유용하게 사용할 수 있는 항생제 마커프리 시스템은 없거나 극히 제한적이다. 영양 요구성 변이주에서 영양요구성 마커(auxotrophic selection marker)를 항생제 마커 대신에 사용할 수도 있지만 이 경우에는 일반적으로 많이 사용되는 산업용 배지인 복합배지를 사용할 수 없다는 단점이 있다.
또 다른 예로 Delphi Genetics사가 개발한 StabyExpress™이 있다(http://www.genengnews.com/gen-articles/protein-bioproduction-without-antibiotics/3891). 이것은 세균에 존재하는 antidote/poison 시스템인 ccd operon (ccdA and ccdB)을 이용하는 것이다. 그러나 이것은 일부 세균에서만 작동을 하고, ccdA / ccdB의 발현 비율이 정확하게 안 맞으면 작동을 안 하는 경우가 빈번하다.
본 발명은 항생제 및 항생제 저항성 마커를 대체할 수 있는 형질전환 생물체 선별용 마커 조성물을 제공하는 것을 목적으로 한다.
본 발명은 항생제 및 항생제 저항성 마커 사용을 요하지 않는 형질전환 방법 및 형질전환 생물체를 제공하는 것을 목적으로 한다.
1. 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로의 효소를 코딩하는 유전자 중 적어도 하나가 도입된 플라스미드를 포함하는 형질전환 생물체 선별용 마커 조성물.
2. 위 1에 있어서, 상기 생물체는 내재적으로 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로를 갖는 것인 형질전환 생물체 선별용 마커 조성물.
3. 위 1에 있어서, 상기 합성 경로는 MEP 경로 또는 MVA 경로인 형질전환 생물체 선별용 마커 조성물.
4. 위 1에 있어서, 상기 합성 경로의 효소를 코딩하는 유전자는 1-디옥시-D-자이룰로스-5-포스페이트(DXP) 신타아제, DXP 리덕토이소머라아제, 2-C-메틸-D-에리스리톨-4-포스페이트(MEP) 시티딜트랜스퍼라아제, 4-디포스포시티딜-2-C-메틸-D-에리스리톨 키나아제, 2-C-메틸-D-에리스리톨-2,4-시클로디포스페이트(MEcPP) 신타아제, 4-히드록시-3-메틸-2-부테닐 디포스페이트 (HMBPP) 신타아제, HMBPP 리덕타아제, 아세토아세틸-CoA 신타아제, 3-히드록실-3-메틸글루타리-CoA (HMG-CoA) 신타아제, HMG-CoA 리덕타아제, 메발로네이트 키나아제, 메발로네이트-5-포스페이트 키나아제, 메발로네이트-5-디포스페이트 디카르복실라아제 및 IPP 아이소머라아제로 이루어진 군에서 선택된 1종 이상의 효소를 코딩하는 유전자인 형질전환 생물체 선별용 마커 조성물.
5. 위 1에 있어서, 상기 조성물은 상기와 동일 효소를 코딩하는 유전자 또는 이의 보완 유전자가 감쇄 또는 결손된 생물체에 형질전환 되는 것인 형질전환 생물체 선별용 마커 조성물.
6. 위 5에 있어서, 상기 유전자는 MEP 경로의 효소를 코딩하는 유전자이고, 상기 보완 유전자는 MVA 경로의 효소 중 적어도 하나를 코딩하는 유전자인 형질전환 생물체 선별용 마커 조성물.
7. 위 6에 있어서, 상기 보완 유전자는 아세토아세틸-CoA 신타아제, 3-히드록실-3-메틸글루타리-CoA (HMG-CoA) 신타아제, HMG-CoA 리덕타아제, 메발로네이트 키나아제, 메발로네이트-5-포스페이트 키나아제, 메발로네이트-5-디포스페이트 디카르복실라아제 및 IPP 아이소머라아제를 코딩하는 유전자인 형질전환 생물체 선별용 마커 조성물.
8. 위 5에 있어서, 상기 유전자는 MVA 경로의 효소를 코딩하는 유전자이고, 상기 보완 유전자는 MEP 경로의 효소 중 적어도 하나를 코딩하는 유전자인 형질전환 생물체 선별용 마커 조성물.
9. 위 1에 있어서, 상기 유전자 중 적어도 2개를 포함하고, 이들은 별개의 플라스미드에 각각 도입된 것인 형질전환 생물체 선별용 마커 조성물.
10. 위 1에 있어서, 상기 플라스미드는 이소프레노이드, 산탈렌, 비사볼롤 및 레티놀 합성 경로 이루어진 군에서 선택된 경로의 효소를 코딩하는 유전자가 더 도입된 것인 형질전환 생물체 선별용 마커 조성물.
11. 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로의 효소를 코딩하는 유전자 중 적어도 하나가 감쇄 또는 결손되고, 상기 감쇄 또는 결손된 유전자와 동일 효소를 코딩하는 유전자 또는 그 보완 유전자가 도입된 플라스미드로 형질전환된 생물체.
12. 위 11에 있어서, 상기 생물체는 내재적으로 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로를 갖는 것인 생물체.
13. 위 11에 있어서, 상기 합성 경로는 MEP 경로 또는 MVA 경로인 생물체.
14. 위 11에 있어서, 상기 합성 경로의 효소를 코딩하는 유전자는 1-디옥시-D-자이룰로스-5-포스페이트(DXP) 신타아제, DXP 리덕토이소머라아제, 2-C-메틸-D-에리스리톨-4-포스페이트(MEP) 시티딜트랜스퍼라아제, 4-디포스포시티딜-2-C-메틸-D-에리스리톨 키나아제, 2-C-메틸-D-에리스리톨-2,4-시클로디포스페이트(MEcPP) 신타아제, 4-히드록시-3-메틸-2-부테닐 디포스페이트 (HMBPP) 신타아제, HMBPP 리덕타아제, 아세토아세틸-CoA 신타아제, 3-히드록실-3-메틸글루타리-CoA (HMG-CoA) 신타아제, HMG-CoA 리덕타아제, 메발로네이트 키나아제, 메발로네이트-5-포스페이트 키나아제, 메발로네이트-5-디포스페이트 디카르복실라아제 및 IPP 아이소머라아제로 이루어진 군에서 선택된 1종 이상의 효소를 코딩하는 유전자인 생물체.
15. 위 11에 있어서, 상기 감쇄 또는 결손되는 유전자는 MEP 경로 상의 효소를 코딩하는 유전자인 생물체.
16. 위 15에 있어서, 상기 유전자는 DXP 신타아제 및 DXP 리덕토이소머라아제 중 적어도 하나를 코딩하는 유전자인 생물체.
17. 위 11에 있어서, 상기 감쇄 또는 결손되는 유전자는 MEP 경로 상의 효소를 코딩하는 유전자이고, 상기 보완 유전자는 MVA 경로 상의 효소 중 적어도 하나를 코딩하는 유전자인 생물체.
18. 위 17에 있어서, 상기 보완 유전자는 아세토아세틸-CoA 신타아제, 3-히드록실-3-메틸글루타리-CoA (HMG-CoA) 신타아제, HMG-CoA 리덕타아제, 메발로네이트 키나아제, 메발로네이트-5-포스페이트 키나아제, 메발로네이트-5-디포스페이트 디카르복실라아제 및 IPP 아이소머라아제를 코딩하는 유전자인 생물체.
19. 위 11에 있어서, 상기 감쇄 또는 결손되는 유전자는 MVA 경로 상의 효소를 코딩하는 유전자인 생물체.
20. 위 11에 있어서, 상기 플라스미드는 이소프레노이드, 산탈렌, 비사볼롤 및 레티놀 합성 경로 이루어진 군에서 선택된 경로의 효소를 코딩하는 유전자가 더 도입된 것인 생물체.
21. 형질전환 대상 균주의 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로의 효소를 코딩하는 유전자 중 적어도 하나를 감쇄 또는 결손시키는 단계; 및
상기 균주를 감쇄 또는 결손된 유전자와 동일 효소를 코딩하는 유전자 또는 이의 보완 유전자가 도입된 재조합 플라스미드로 형질전환시키는 단계;를 포함하는 생물체의 형질 전환 방법.
22. 위 21에 있어서, 상기 생물체는 내재적으로 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로를 갖는 것인 생물체의 형질 전환 방법.
23. 위 21에 있어서, 상기 형질 전환은 항생제 없이 수행되는 것인 생물체의 형질 전환 방법.
24. 위 21에 있어서, 상기 합성 경로는 MEP 경로 또는 MVA 경로인 생물체의 형질 전환 방법.
25. 위 21에 있어서, 상기 합성 경로의 효소를 코딩하는 유전자는 1-디옥시-D-자이룰로스-5-포스페이트(DXP) 신타아제, DXP 리덕토이소머라아제, 2-C-메틸-D-에리스리톨-4-포스페이트(MEP) 시티딜트랜스퍼라아제, 4-디포스포시티딜-2-C-메틸-D-에리스리톨 키나아제(IspE), 2-C-메틸-D-에리스리톨-2,4-시클로디포스페이트(MEcPP) 신타아제, 4-히드록시-3-메틸-2-부테닐 디포스페이트 (HMBPP) 신타아제, HMBPP 리덕타아제, 아세토아세틸-CoA 신타아제, 3-히드록실-3-메틸글루타리-CoA (HMG-CoA) 신타아제, HMG-CoA 리덕타아제, 메발로네이트 키나아제, 메발로네이트-5-포스페이트 키나아제, 메발로네이트-5-디포스페이트 디카르복실라아제 및 IPP 아이소머라아제 로 이루어진 군에서 선택된 1종 이상의 효소를 코딩하는 유전자인 생물체의 형질 전환 방법.
26. 위 21에 있어서, 상기 감쇄 또는 결손되는 유전자는 MEP 경로 상의 효소를 코딩하는 유전자인 생물체의 형질 전환 방법.
27. 위 26에 있어서, 상기 유전자는 DXP 신타아제 및 DXP 리덕토이소머라아제 중 적어도 하나를 코딩하는 유전자인 생물체의 형질 전환 방법.
28. 위 21에 있어서, 상기 감쇄 또는 결손되는 유전자는 MEP 경로 상의 효소를 코딩하는 유전자이고, 상기 보완 유전자는 MVA 경로 상의 효소 중 적어도 하나를 코딩하는 유전자인 생물체의 형질 전환 방법.
29. 위 28에 있어서, 상기 보완 유전자는 아세토아세틸-CoA 신타아제, 3-히드록실-3-메틸글루타리-CoA (HMG-CoA) 신타아제, HMG-CoA 리덕타아제, 메발로네이트 키나아제, 메발로네이트-5-포스페이트 키나아제, 메발로네이트-5-디포스페이트 디카르복실라아제 및 IPP 아이소머라아제를 코딩하는 유전자인 생물체의 형질 전환 방법.
30. 위 21에 있어서, 상기 감쇄 또는 결손되는 유전자는 MVA 경로 상의 효소 중 적어도 하나를 코딩하는 유전자인 생물체의 형질 전환 방법.
31. 위 21에 있어서, 상기 플라스미드는 이소프레노이드, 산탈렌, 비사볼롤 및 레티놀 합성 경로 이루어진 군에서 선택된 경로의 효소를 코딩하는 유전자를 더 포함하는 생물체의 형질 전환 방법.
32. 위 21에 있어서, 적어도 2개의 유전자를 감쇄 또는 결손시키고, 상기 균주를 이와 동일한 효소를 코딩하는 유전자를 각각 포함하는 적어도 2개의 플라스미드로 형질전환시키는 생물체의 형질 전환 방법.
33. 위 11 내지 20 중 어느 한 항의 생물체를 기질이 포함된 배지 상에서 배양하는 단계를 포함하는 목적 산물의 생산 방법.
34. 위 33에 있어서, 상기 배지는 항생제를 포함하지 않는 것인 목적 산물의 생산 방법.
본 발명의 형질전환 생물체 선별용 마커 조성물은 항생제 저항성 유전자를 사용하지 않는다. 이에, 항생제 및 항생제 저항성 유전자의 사용 없이도 형질 전환이 가능하여, 항생제 및 항생제 저항성 유전자 사용에 기인하는 많은 문제들을 원천적으로 방지할 수 있다.
본 발명의 형질전환 생물체 선별용 마커 조성물은 생물체의 장시간 배양에도 소실이 적다.
본 발명의 생물체는 항생제, 항생제 저항성 유전자 없이 형질전환 및 목적 산물의 생산이 가능하여, 항생제 및 항생제 저항성 유전자 사용에 기인하는 많은 문제들을 원천적으로 방지할 수 있다.
본 발명의 형질전환 방법은 항생제, 항생제 저항성 유전자 없이 형질전환이 가능하다.
본 발명의 목적 산물의 생산 방법은 높은 수율로 목적 산물을 생산할 수 있다.
도 1은 IPP와 DMAPP 생합성 경로이다.
도 2는 레티노이드의 HPLC 분석 프로파일(Profile)이다.
도 3은 레티노이드 정량분석을 위한 표준곡선이다.
도 4는 산탄렌의 GC 분석 프로파일이다.
도 5는 비사볼롤의 GC 분석 프로파일이다.
도 6은 비사볼롤 정량분석을 위한 표준곡선
도 7은 발효 부산물 생성 경로이다.
도 8은 MEP 경로 결손주에서 외래 MVA 경로를 보완하여 구축한 항생제 불필요 산탈렌 생산 균주의 항생제 유무에 따른 배양 결과이다.
도 9은 MEP 경로 결손주에서 외래 MVA 경로를 두 플라스미드로 나누어 구축한 항생제가 불필요한 레티노이드 생산 균주의 항생제 유무에 따른 배양 결과이다.
도 10은 항생제 마커가 제거된 레티노이드 생산 균주의 항생제 유무에 따른 배양 결과이다.
도 11은 결손된 MEP 경로 유전자를 선별마커로 이용한 산탈렌 생산 균주의 항생제 유무에 따른 배양 결과이다.
도 12는 결손된 MEP 경로 유전자를 복수 플라스미드에 나누어 구축하여 항생제가 불필요한 이소프레노이드(산탈렌, 비사볼롤) 생산 균주의 배양 결과이다.
도 13은 결손된 MEP 경로 유전자와 함께 외래 MVA 경로가 추가 도입된 항생제 마커가 제거된 레티노이드 생산 균주의 배양 결과이다.
도 14는 결손된 MEP 경로 유전자를 선별마커로 하여 항생제가 불필요한 균주의 형광 단백질 발현 결과이다.
도 15는 pCIN-mvaA-EGFP 재조합 플라스미드 모식도이다.
도 16은 결손된 MVA 경로 유전자를 선별마커로하여 항생제가 불필요한 균주의 형광 단백질 발현 결과이다.
도 2는 레티노이드의 HPLC 분석 프로파일(Profile)이다.
도 3은 레티노이드 정량분석을 위한 표준곡선이다.
도 4는 산탄렌의 GC 분석 프로파일이다.
도 5는 비사볼롤의 GC 분석 프로파일이다.
도 6은 비사볼롤 정량분석을 위한 표준곡선
도 7은 발효 부산물 생성 경로이다.
도 8은 MEP 경로 결손주에서 외래 MVA 경로를 보완하여 구축한 항생제 불필요 산탈렌 생산 균주의 항생제 유무에 따른 배양 결과이다.
도 9은 MEP 경로 결손주에서 외래 MVA 경로를 두 플라스미드로 나누어 구축한 항생제가 불필요한 레티노이드 생산 균주의 항생제 유무에 따른 배양 결과이다.
도 10은 항생제 마커가 제거된 레티노이드 생산 균주의 항생제 유무에 따른 배양 결과이다.
도 11은 결손된 MEP 경로 유전자를 선별마커로 이용한 산탈렌 생산 균주의 항생제 유무에 따른 배양 결과이다.
도 12는 결손된 MEP 경로 유전자를 복수 플라스미드에 나누어 구축하여 항생제가 불필요한 이소프레노이드(산탈렌, 비사볼롤) 생산 균주의 배양 결과이다.
도 13은 결손된 MEP 경로 유전자와 함께 외래 MVA 경로가 추가 도입된 항생제 마커가 제거된 레티노이드 생산 균주의 배양 결과이다.
도 14는 결손된 MEP 경로 유전자를 선별마커로 하여 항생제가 불필요한 균주의 형광 단백질 발현 결과이다.
도 15는 pCIN-mvaA-EGFP 재조합 플라스미드 모식도이다.
도 16은 결손된 MVA 경로 유전자를 선별마커로하여 항생제가 불필요한 균주의 형광 단백질 발현 결과이다.
이하 본 발명을 상세히 설명한다.
본 발명은 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로의 효소를 코딩하는 유전자 중 적어도 하나가 도입된 플라스미드를 포함하는 형질전환 생물체 선별용 마커 조성물을 제공한다.
이소펜테닐 디포스페이트(IPP) 또는 디메틸알릴 디포스페이트(DMAPP) 합성 경로는 모든 생명체가 필수적으로 갖고 있는 생합성 경로로서, IPP, DMAPP는 세포 내 필수 대사물질로 결핍이 되면 세포는 생장을 할 수 없다. 또한, 이들 물질은 강력한 음의 전하를 갖는 이인산화 물질로 배지 내에 존재해도 세포 내로 유입될 수가 없어서 반드시 세포 내에서 만들어져야 한다.
이에, 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로의 효소를 코딩하는 유전자 중 적어도 하나가 도입된 플라스미드를 포함하는 경우에 이를 형질전환 생물체 선별용 마커 조성물로 사용될 수 있다.
본 발명에 따른 생물체는 상기 이소펜테닐 디포스페이트(IPP) 또는 디메틸알릴 디포스페이트(DMAPP) 합성 경로를 내재적으로 가지는 것이라면 그 종류는 제한되지 않으며, 동물, 식물, 미생물을 포함하는 것이며, 구체적으로는 미생물일 수 있다.
이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로는 구체적으로 도 1에 도시된 MEP 경로 또는 MVA 경로일 수 있다.
이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로의 효소를 코딩하는 유전자는 예를 들면 MEP 경로의 1-디옥시-D-자이룰로스-5-포스페이트(DXP) 신타아제(1-deoxy-D-xylulose-5-phosphate synthase), DXP 리덕토이소머라아제(DXP reductoisomerase), 2-C-메틸-D-에리스리톨-4-포스페이트(MEP) 시티딜트랜스퍼라아제(2-C-methyl-D-erythritol-4-phosphate (MEP) cytidylyltransferase), 4-디포스포시티딜-2-C-메틸-D-에리스리톨 키나아제(4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, IspE), 2-C-메틸-D-에리스리톨-2,4-시클로디포스페이트(MEcPP) 신타아제(2-C-methyl-D-erythritol-2,4-cyclodiphosphate synthase), 4-히드록시-3-메틸-2-부테닐 디포스페이트 (HMBPP) 신타아제(4-hydroxy-3-methyl-2-butenyl diphosphate synthase), HMBPP 리덕타아제(HMBPP reductase), MVA 경로의 아세토아세틸-CoA 신타아제(acetoacetyl-CoA synthase), 3-히드록실-3-메틸글루타리-CoA (HMG-CoA) 신타아제(3-hydroxyl-3-methylglutary-CoA synthase), HMG-CoA 리덕타아제(HMG-CoA reductase), 메발로네이트 키나아제(mevalonate kinase), 메발로네이트-5-포스페이트 키나아제(mevalonate-5-phosphate kinase), 메발로네이트-5-디포스페이트 디카르복실라아제(mevalonate-5-diphosphate decarboxylase) 및 IPP 아이소머라아제(IPP isomerase) 등의 유전자를 들 수 있다.
본 발명의 형질전환 생물체 선별용 마커 조성물은 상기와 동일 효소를 코딩하는 유전자 또는 이의 보완 유전자가 감쇄 또는 결손된 생물체에 형질전환 되는 것일 수 있다.
전술한 바와 같이, 이소펜테닐 디포스페이트 및 디메틸알릴 디포스페이트 합성 경로가 불활성화되면 세포가 생존할 수 없게 되는데, 본 발명의 마커 조성물은 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로의 효소를 코딩하는 유전자 중 적어도 하나가 도입된 플라스미드를 포함하여, 이로 형질전환된 생물체는 결손된 IPP/DMAPP 경로가 다시 활성화되어 생존 가능하므로, 생물체의 생존 여부로 형질 전환 생물체를 확인할 수 있다.
형질전환 대상 생물체는 마커 조성물의 유전자와 동일 효소를 코딩하는 유전자 또는 이의 보완 유전자가 감쇄 또는 결손된 것일 수 있다.
동일 효소를 코딩하는 유전자인 경우 감쇄 또는 결손된 유전자와 동일한 유전자일 수도 있고, 다른 종 유래의 동일 효소를 코딩하는 유전자일 수도 있다.
구체적인 예를 들자면, 형질전환 대상 생물체는 DXP 리덕토이소머라아제를 코딩하는 유전자가 감쇄 또는 결손된 것일 수 있고, 본 발명에 따른 플라스미드는 그와 동일한 유전자 또는 다른 종 유래(유전자는 상이)의 동일한 효소를 코딩하는 유전자가 도입된 것일 수 있다.
보완 유전자는 유전자의 감쇄 또는 결손에 의해 불활성화된 경로 이외의 경로를 활성화시켜 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트를 생산할 수 있도록 하는 유전자를 의미한다. 예를 들면 생물체가 MEP 경로의 유전자가 감쇄 또는 결손된 경우에 마커 조성물의 플라스미드는 MVA 경로 전체의 효소를 코딩하는 유전자가 도입된 것일 수 있고, 생물체가 MVA 경로의 유전자가 감쇄 또는 결손된 경우에 마커 조성물의 플라스미드는 MEP 경로 전체의 효소를 코딩하는 유전자가 도입된 것일 수 있다.
본 발명의 조성물이 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로의 효소를 코딩하는 유전자 중 적어도 2개 이상이 도입된 플라스미드를 포함하는 경우에, 하나의 플라스미드에 2개 이상의 유전자가 도입된 것일 수도 있고, 별개의 플라스미드에 각각 유전자가 도입된 것일 수도 있다.
별개의 플라스미드에 각각 유전자가 도입된 경우에는 생물체가 모든 플라스미드로 형질전환되어야 생존할 수 있으므로, 생존하는 생물체를 모든 플라스미드로 형질전환된 생물체로서 선별할 수 있다.
구체적인 예를 들자면, 형질전환 대상 생물체는 DXP 신타아제를 코딩하는 유전자 및 DXP 리덕토이소머라아제를 코딩하는 유전자 중 적어도 하나가 감쇄 또는 결손된 것일 수 있고, 본 발명의 조성물은 그와 동일한 유전자 또는 다른 종 유래(유전자는 상이)의 동일한 효소를 코딩하는 유전자가 각각 도입된 2개의 플라스미드를 포함할 수 있다.
통상 생물체의 형질전환은 항생제가 존재하는 배양액 상에서 수행되어, 항생제 저항성 유전자가 선택 마커로서 사용되나, 그러한 경우에 고가의 항생제 사용에 의한 생산 비용 상승, 항생제 유출에 따른 환경 오염, 항생제 저항성 돌연변이 생물체 발생 위험 등의 문제가 있다.
또한, 항생제 저항성 유전자가 숙주 세포를 완벽하게 보호하는 것은 아니므로, 항생제에 의한 숙주 세포의 손상이 발생할 수 있고, 이를 최소화하는 항생제 사용 최적 농도를 설정하고 이를 유지하는 것은 산업계에서 매우 어려운 일로 인식되며, 이는 숙주 세포의 변경마다 달라질 수 있다.
더욱이, 항생제 사용 배양의 경우에 배양 시간이 길어지면 항생제의 분해 및 변형으로 인해서 항생제 저항성 유전자를 선택마커로 갖고 있는 플라스미드의 소실이 발생하고 배양 후반기의 생산성의 급격한 감소를 초래할 수 있다. 항생제의 분해 및 변형은 항생제 마커 유전자에서 발현된 효소에 의한 것과 항생제의 불안정성에 의한 자연발생적인 것으로 2차 산물 생산 등의 장시간 발효를 요하는 배양공정에서 심각한 문제를 유발한다.
그러나, 본 발명의 상기 형질전환 생물체 선별용 마커 조성물을 사용함으로써 상기 문제점들의 발생을 원천적으로 방지하고, 항생제를 사용하지 않고도 플라스미드를 숙주 생물체 내에서 안전하게 유지하고, 형질전환 생물체를 선별할 수 있다.
또한, 그러한 플라스미드에 목적 산물 생산을 위한 경로의 효소를 코딩하는 유전자를 추가로 도입하여, 목적 산물을 숙주 생물체에서 안정적으로 대량 생산하는 데에 활용할 수도 있다.
또한, 본 발명은 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로의 효소를 코딩하는 유전자 중 적어도 하나가 감쇄 또는 결손되고, 상기 감쇄 또는 결손된 유전자와 동일한 효소를 코딩하는 유전자 또는 그 보완 유전자가 도입된 플라스미드로 형질전환된 생물체를 제공한다.
이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로는 MEP 경로 또는 MVA 경로일 수 있고, 이들 효소는 전술한 바와 같다.
본 발명의 생물체에서 감쇄 또는 결손되는 유전자는 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로의 효소를 코딩하는 유전자 중 적어도 하나, 구체적으로 MEP 경로 또는 MVA 경로 상의 효소를 코딩하는 유전자 중 적어도 하나일 수 있다.
본 발명의 생물체는 감쇄 또는 결손된 유전자와 동일한 유전자 또는 다른 종 유래의 동일한 효소를 코딩하는 유전자가 도입된 플라스미드로 형질전환된 것일 수 있다.
구체적인 예를 들자면, DXP 신타아제 및 DXP 리덕토이소머라아제를 코딩하는 유전자 중 적어도 하나가 감쇄 또는 결손된 것일 수 있고, 그와 동일한 유전자 또는 다른 종 유래(유전자는 상이)의 동일한 효소를 코딩하는 유전자가 도입된 플라스미드로 형질전환된 것일 수 있다.
또한, 본 발명의 생물체는 감쇄 또는 결손된 유전자의 보완 유전자가 도입된 플라스미드로 형질전환된 것일 수 있다.
보완 유전자는 유전자의 감쇄 또는 결손에 의해 불활성화된 경로 이외의 경로를 활성화시켜 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트를 생산할 수 있도록 하는 유전자를 의미한다. 예를 들면 생물체가 MEP 경로의 유전자가 감쇄 또는 결손된 경우에 MVP 경로 전체의 효소를 코딩하는 유전자가 도입된 플라스미드로 형질전환된 것일 수 있고, 생물체가 MVA 경로의 유전자가 감쇄 또는 결손된 경우에 MEP 경로 전체의 효소를 코딩하는 유전자가 도입된 플라스미드로 형질전환된 것일 수 있다.
본 발명의 생물체는 목적 산물 생산을 위한 목적 산물 합성 경로의 효소를 코딩하는 유전자로 더 형질전환된 것일 수 있다.
그 유전자는 목적하는 산물에 따라 다양하게 선택될 수 있으며, 예를 들면 이소프레노이드 합성 경로, 산탈렌 합성 경로, 레티놀 합성 경로, 비사볼롤 합성 경로 등을 들 수 있으나, 이에 제한되는 것은 아니다. 상기 각 합성 경로는 모두 공지된 경로로서, 공지된 경로의 효소를 코딩하는 유전자로 형질전환될 수 있다.
또한, 본 발명의 생물체는 상기 목적 산물 합성 경로에서 목적 산물의 부산물 생성 경로의 효소를 코딩하는 유전자가 감쇄 또는 결실된 것일 수 있다. 이에 의해 목적 산물 생산 수율이 더욱 향상될 수 있다. 예를 들면 MVA 경로의 출발 물질인 acetyl-CoA를 발효부산물인 acetate, lactate, ethanol로 전환시키는 효소들을 들 수 있고, 구체적으로 아세트알데히드 디하이드로게나아제(adhE), 파루베이트 옥시다아제(PoxB), 락테이트 디하이드로게나아제(ldhA), 아세틸-COA:아세토아세틸-CoA 신타아제(atoDA) 등을 들 수 있으나, 이에 제한되는 것은 아니다(도 7 참조).
본 발명의 생물체는 항생제 저항성 유전자를 포함하지 않을 수 있다. 항생제 저항성 유전자를 마커로 사용하지 않으므로 이를 포함하는 것이 불필요하다. 물론, 전술한 형질전환 이후에 추후 항생제 저항성 유전자가 도입된 플라스미드로 추가로 형질전환되어 항생제 저항성 유전자를 포함할 수도 있다.
본 발명은 형질전환 대상 생물체의 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로의 효소를 코딩하는 유전자 중 적어도 하나를 감쇄 또는 결손시키는 단계; 및 상기 생물체를 감쇄 또는 결손된 유전자와 동일한 효소를 코딩하는 유전자 또는 그 보완 유전자가 도입된 재조합 플라스미드로 형질전환시키는 단계;를 포함하는 생물체의 형질 전환 방법을 제공한다.
이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로의 효소를 코딩하는 유전자는 전술한 범위 내의 유전자일 수 있다.
전술한 바와 같이 통상 생물체의 형질 전환은 항생제를 포함한 배양액 상에서 수행되는 것이지만, 본 발명의 형질 전환 방법은 항생세 저항성 마커를 사용하지 않으므로, 본 발명의 형질 전환은 항생제 없이 수행될 수 있다.
상기 감쇄 또는 결손되는 유전자는 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로의 효소를 코딩하는 유전자 중 적어도 하나일 수 있다. 구체적으로, MEP 경로 또는 MVA 경로 상의 효소를 코딩하는 유전자일 수 있다.
형질전환시에는 감쇄 또는 결손된 유전자와 동일한 유전자 또는 다른 종 유래의 동일한 효소를 코딩하는 유전자가 도입된 플라스미드로 형질전환시킬 수 있다.
구체적인 예를 들자면, DXP 신타아제 및 DXP 리덕토이소머라아제 중 적어도 하나를 코딩하는 유전자가 감쇄 또는 결손될 수 있고, 그와 동일한 유전자 또는 다른 종 유래(유전자는 상이)의 동일한 효소를 코딩하는 유전자가 도입된 플라스미드로 형질전환될 수 있다.
또한, 감쇄 또는 결손된 유전자의 보완 유전자가 도입된 플라스미드로 형질전환될 수 있다.
보완 유전자는 유전자의 감쇄 또는 결손에 의해 불활성화된 경로 이외의 경로를 활성화시켜 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트를 생산할 수 있도록 하는 유전자를 의미한다. 예를 들면 생물체가 MEP 경로의 유전자가 감쇄 또는 결손된 경우에 MVP 경로 전체의 효소를 코딩하는 유전자가 도입된 플라스미드로 형질전환 될 수 있고, 생물체가 MVA 경로의 유전자가 감쇄 또는 결손된 경우에 MEP 경로 전체의 효소를 코딩하는 유전자가 도입된 플라스미드로 형질전환 될 수 있다.
2개 이상의 유전자가 도입되는 경우에 이들은 단일 플라스미드에 도입될 수도 있고, 복수개의 플라스미드에 각각 도입될 수도 있다.
비제한적인 보다 구체적인 결손 및 형질전환 방법의 예시를 들자면, 전술한 바와 같이 IPP, DMAPP가 없으면 생물체가 생존할 수 없으므로, MEP 경로의 dxs 또는 ispC의 결손시에 배지에 2-C-메틸-D-에리스리톨을 첨가하여 ispC의 대사산물인 2-C-메틸-D-에리스리톨 4-포스페이트(MEP)를 생성하여 생물체의 생육을 유지시킨 상태에서 상기 생물체를 이와 동일한 효소를 코딩하는 유전자 또는 그 보완 유전자로 형질전환시킬 수 있다. 또한, 먼저 MVA 하부 경로 유전자를 도입하고, 메바론산이 첨가된 배지에서 MEP 경로 유전자의 감쇄 또는 결손을 수행할 수도 있다.
MVA 경로 유전자의 결손시에는 상부 경로, 하부 경로 감쇄 또는 결손에 따라 구체적인 방법이 달라질 수 있다.
상부 경로 결손의 구체적인 예를 들자면 배지에 메바론산을 첨가하여 하부 경로만으로 생육을 유지시킨 상태에서, 상부 경로 유전자를 감쇄 또는 결손시키고 이와 동일한 효소를 코딩하는 유전자 또는 그 보완 유전자로 형질전환시킬 수 있다.
하부 경로 결손의 구체적인 예를 들자면, MEP 경로 유전자 모두를 도입하여 생육을 유지시킨 상태에서, 상부 경로 유전자를 감쇄 또는 결손시키고 이와 동일한 효소를 코딩하는 유전자 또는 그 보완 유전자로 형질전환시킬 수 있다.
본 발명에 따른 플라스미드는 목적 산물 생산을 위한 목적 산물 생산 경로의 효소를 코딩하는 유전자를 더 포함할 수 있다.
그 유전자는 목적하는 산물에 따라 다양하게 선택될 수 있으며, 예를 들면 이소프레노이드 합성 경로, 산탈렌 합성 경로, 레티놀 합성 경로, 비사볼롤 합성 경로 등을 들 수 있으나, 이에 제한되는 것은 아니다. 상기 각 합성 경로는 모두 공지된 경로로서, 공지된 경로의 효소를 코딩하는 유전자가 더 도입될 수 있다.
또한, 본 발명에 따른 생물체는 상기 목적 산물 합성 경로에서 목적 산물의 부산물 생성 경로의 효소를 코딩하는 유전자가 감쇄 또는 결실된 것일 수 있다. 이에 의해 목적 산물 생산 수율이 더욱 향상될 수 있다. 예를 들면 MVA 경로의 출발 물질인 acetyl-CoA를 발효부산물인 acetate, lactate, ethanol로 전환시키는 효소들을 들 수 있고, 구체적으로 아세트알데히드 디하이드로게나아제(adhE), 파루베이트 옥시다아제(PoxB), 락테이트 디하이드로게나아제(ldhA), 아세틸-COA:아세토아세틸-CoA 신타아제(atoDA) 등을 들 수 있으나, 이에 제한되는 것은 아니다(도 7 참조).
또한, 본 발명은 상기 생물체를 이용한, 또는 상기 형질전환 방법을 포함하는 목적 산물의 생산 방법을 제공한다.
본 발명의 목적 산물의 생산 방법은 상기 생물체를 목적 산물 생산 경로의 효소를 코딩하는 유전자로 형질전환시켜 목적 산물을 생산한다.
목적 산물 생산 경로의 효소를 코딩하는 유전자는 전술한 플라스미드에 도입되어 생물체에 형질전환될 수 있다.
목적 산물은 상기 생물체를 기질을 포함하는 배지 중에서 배양하여 생산할 수 있고, 배양은 항생제를 포함하지 않는 배양 조건에서 수행될 수 있다.
본 발명의 방법은 항생제 및 항생제 저항성 유전자 없이도 형질전환 및 목적 산물의 생산이 가능하여, 항생제 사용에 의한 문제를 원천적으로 방지할 수 있고, 항생제의 분해 및 변형에 의한 플라스미드의 소실 문제가 발생하지 않으므로, 보다 높은 수율로 목적 산물을 생산할 수 있다.
이하, 본 발명을 구체적으로 설명하기 위해 실시예를 들어 상세하게 설명하기로 한다.
실시예
1. 재료 및 방법
1) 실험균주 및 재료
실험에 이용된 미생물은 ATCC (American Type Culture Collection), KCTC (Korea Collection for Type Cultures), 및 KCCM (Korea Culture Center of Microorganisms)에서 구입하였으며, 표 1에 정리하였다.
| 실험균주 | 설명 | 정보 또는 참고문헌 |
| DH5a | F-, λ-, endA1, glnV44, thi-1, recA1, relA1, gyrA96, deoR, φ80, dlacZ △15, △lacZYA-argF) U169, hsdR17(rK - mK +), supE44 |
NEB C2987 |
| MG1655(DE3) | F- λ- ilvG rfb-50 rph-1 (DE3) | KCCM 41310 |
| MG1655(DE3) DadhE ::MVA bottom | DadhE (::PcTrc-SN12Didi-ter) | - |
| MG1655(DE3) DatoDA::MVA bottom | DatoDA (::PcTrc-SN12Didi-ter) | - |
| MG1655(DE3) DldhA::MVA bottom | DldhA (::PcTrc-SN12Didi-ter) | - |
| MG1655(DE3) DpoxB::MVA bottom | DpoxB (::PcTrc-SN12Didi-ter) | - |
| MG1655(DE3) Δdxr DadhE ::MVA bottom |
Δdxr DadhE (::PcTrc-SN12Didi-ter) | - |
| MG1655(DE3) Δdxr/s DadhE ::MVA bottom |
Δdxr, Δdxs, DadhE (::PcTrc-SN12Didi-ter) | - |
| E. coli EC1000 | RepA+ MC1000, KmR , carrying a single copy of the pWV01 repA gene in the glgB gene; host for pOR128-based plasmids | Leenhouts et al Mol Gen Genet. 1996 Nov 27;253(1-2):217-24 |
| L. lactis subsp. cremoris MG1363 | Plasmid-free derivative of L. lactis subsp. Cremoris NCDO712 | Plasmid complement of Streptococcus lactis NCDO712 and other lactic streptococci after protoplast-induced curing. J. Bacteriol.154:1-9. |
| L. lactis subsp. cremoris MG1363 ΔmvaA | ΔmvaA | - |
유전자 클로닝에는 DH5α(F-f80dlacZDM15D(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK-, mK+) phoA supE44λ- thi-1 gyrA96 relA1)를 이용하였고, 목적물질 생산에는 MG1655(DE3)(F- λ- ilvG rfb-50 rph-1(DE3))를 사용하였다. 발현벡터로는 pTrc99A, pSTV28을 사용하였다(표 2). 제한효소 및 기타 효소는 New England Biolabs (미국) 제품을 사용하였다. 또한 PCR을 수행하기 위해서는 Pfu-X DNA polymerase는 Solgent (한국), Phusion DNA polymerase는 Thermo Scientific (미국) 제품을 사용하였다. DNA size maker는 Invitrogen (미국) 제품을 사용하였다. IPTG는 Promega(미국), L(+)-arabinose, glucose, lactose는 Sigma (미국), acetone은 Merck (미국), glycerol은 Amresco (미국) 제품을 사용하였다. 그 외 시약들은 Sigma (미국)의 제품을 사용하였다.
미생물 배양을 위한 배지의 제조는 각 균주 분양기관의 권장배지조성 및 Difco manual (11th edition, Difco; BD Science, US)에 따랐다. 배지 제조에 사용된 시약은 BD Science (미국) 및 Sigma (미국)로부터 구입하였다. 배양 중의 균체량은 spectrophotometer (Shimadzu UV-1601, Japan)로 600 nm에서 OD (optical density)를 측정한 결과로 나타내었으며, pH는 pH meter B-212 (HORIBA, Japan)로 측정하였다.
유전자 클로닝에는 플라스미드 유지를 위한 항생제로 ampicillin, kanamycin을 사용하였으며, 각각 100 ㎍/mL, 50 ㎍/mL 농도로 사용하였다.
2) 레티노이드의 추출 및 분석
레티노이드는 다음의 방법으로 분석하였다. 배양액 50 ~ 100 ㎕를 취해서 14,000 rpm에서 40초 동안 원심분리하여 균체를 회수하였다. 회수된 균체에 아세톤 400 ㎕을 첨가하여 균체를 재현탁시킨 다음 어두운 곳에서 55℃에서 15분 동안 추출하고, 이에 다시 600 ㎕의 아세톤을 첨가하여 55℃에서 15분 동안 추출하였다. 추출물은 14,000 rpm에서 10분 동안 원심분리 한 후 상층액만을 취하여 HPLC 정량 분석을 실시하였다. 그리고 레티노이드 배양액에 heavy mineral oil 층을 첨가한 경우 heavy mineral oil 층만을 14,000 rpm 에서 10분 동안 원심분리한 후 레티노이드를 함유하고 있는 heavy mineral oil 층 5 ~ 50 ㎕를 1 mL 아세톤에 재현탁하여 추출하였으며, 상온에서 15분간 방치하며 5분 간격으로 vortexing하였다. 추출물은 14,000 rpm에서 10분 동안 원심분리 한 후 acetone 층만을 취하여 HPLC 정량 분석을 실시하였다.
레티노이드의 분석 시스템은 SHIMADZU LC-20A series with UV/Vis detector (Shimadzu, Kyoto, Japan)을 이용하였으며 분석칼럼은 Symmetry C18 (250x4.6,5 ㎛) with Symmetry guard C18 (15x4.6, 5 ㎛)을 사용하였다. 이동상은 메탄올: 아세토니트릴 혼합용액(95:5, v/v)으로 총 15분간 분석하였다. 유속은 1.5 mL/min으로 하였으며 검출 파장은 각각 레티날은 370 nm, 레티놀과 레티닐 아세테이트는 340 nm에서 측정하였고, 시료 주입량은 20 ㎕, 오븐 온도는 40℃로 분석하였다. 레티노이드 표준시료는 에탄올에 녹여 사용하였다. 표준시간의 피크 머무름 시간은 레티놀 약 3.2분, 레티날 약 3.4분, 레티닐 아세테이트는 약 4.0분이며(도 2), 분석결과는 분석된 피크의 면적을 표준시료의 피크 면적으로 계산한 검량선에 대입하고 희석배수를 환산하여 계산하였다(도 3).
3) 산탈렌의 추출 및 분석
배지에 데케인을 도포하는 2상 배양에서 생산된 산탈렌 분석을 위해서 데케인 층의 gas chromatography (GC) 와 gas chromatography-mass spectrometry (GC-MS) 분석을 실시하였다(도 4). GC 분석은 19091N-133 HP-Innowax column(30 m; internal diameter, 0.25 mm; film thickness, 250 nm)이 장착된 GC/FID (Agilent Technologies7890A)를 이용하였다. 컬럼 오븐 온도는 초기 80℃에서 1 min, 그리고 10℃/min의 속도로 250℃까지 올리고 1분 동안 유지하였다. 이동상 기체로 질소를 39 psi의 압력으로 흘려주고, detector temperature는 260℃로 유지하였다. GC-MS 분석은 GCMS-QP2010 Ultra (SHIMADU, Tokyo, Japan)를 사용하였고 이동상 기체로는 helium을 이용하였다.
4) 비사볼롤의 추출 및 분석
배양 종료 후 데칸층을 원심분리(14,000 rpm, 10 min)를 통해 회수하여 gas chromatography (GC) 와 gas chromatography-mass spectrometry (GC-MS)로 분석하였다(도 5). GC 분석은 19091N-133 HP-Innowax column (30 m; internal diameter, 0.25 mm; film thickness, 250 nm)이 장착된 GC/FID (Agilent Technologies 7890A)를 이용하였다. 컬럼 오븐 온도는 초기 50℃에서 20℃/min의 속도로 증가시켰으며, 90℃ 도달 이후 15℃/min의 속도로 증가시켰다. 온도가 150℃에 도달한 후 20℃/min 속도로 190℃까지 온도를 증가 시킨 후 10℃/min의 속도로 260℃에 도달하게 한 후 2분 동안 유지하게 하였다. 이동상 기체로 질소를 30 psi의 압력으로 공급하였고, 검출기 온도는 280℃로 유지하였다. GC-MS 분석은 GCMS-QP2010 Ultra (SHIMADU, Japan)를 사용하였고 이동상 기체로는 헬륨을 이용하였다. 측정된 비사볼롤의 피크 면적 값은 미리 작성한 검량선(도 6)에 따라 다음과 같이 계산하였다.
[수학식 1]
α-Bisabolol (mg/L) = 0.8116 × GC Peak area × 희석배수
2. MEP 경로 결손 균주 구축 과정
- MEP 경로 결손 균주 구축 시 MVA 하부경로 유전자를 염색체에 도입하고 구축 작업은 메바론산 첨가 배지에서 진행한 예시
- MEP 상부경로 유전자 결손
1) 대장균 염색체 내 외래 MVA 하부경로 삽입
대장균 염색체 내 외래경로 삽입을 위한 방법은 λ-Red recombinase를 이용한 PCR 기반의 상동성 재조합 방법과 P1 형질도입 방법으로 크게 두 가지가 있다. 이번 실험에서는 λ-Red recombinase를 이용한 PCR 기반의 상동성 재조합 방법을 이용하여 외래 MVA 하부경로를 대장균 MG1655(DE3) 균주에 삽입해주었다. MVA 경로에서 mevalonate 이후부터 DMAPP까지의 경로를 하부 경로로 하였다(도 1 참조). 현재 MVA 하부경로는 Streptococcus pneumoniae의 mvaK1, mvaK2, mvaD와 E. coli의 idi 유전자로 이루어져 있으며, 서열번호 1과 서열번호 2의 프라이머를 이용해 상기의 유전자를 증폭해 pTFCC(DPB) 벡터에 클로닝하여 pTFCC(DPB)-SN12Didi를 구축하였다. MVA 하부경로 삽입위치는 adhE, poxB, ldhA, atoDA 네 가지 유전자에 각각 삽입하였다. 이는 MVA 경로의 출발물질인 acetyl-CoA를 발효부산물인 acetate, lactate, ethanol로 전환시키는 유전자들로 이를 차단하여 발효생산성 및 탄소원 이용효율을 향상시킬 수 있다(도 7).
실험방법은 다음과 같다. 먼저 대장균 염색체 내에 삽입할 경로가 균체 내에서 발현할 수 있도록 promoter, multi-cloning site, terminator, FRT site, 항생제 마커를 포함한 벡터를 구축하였으며, 이는 표 2에 나타내었다.
| 플라스미드 | 설명 | Genbank 허가번호 | 제품 | 서열 번호 |
| pSTV28 | p15A origin, lac promoter, lacZ, and cat | M22744 | Takara Korea (한국) | 71 |
| pTrc99A | pMB1 origin, trc promoter, lacI q , and bla | U13872.1 | Pharmacia (US) | 72 |
| pTFKC(DPB) | ColE1 origin, trc promoter without operator region, kanamycin cassette with FRT, and bla | - | 73 | |
| pTFCC(DPB) | ColE1 origin, trc promoter without operator region, chloramphenicol cassette with FRT, and bla | - | 74 | |
| pCP20 | cI857λts, FLP, cat, and bla | - | 75 | |
| pKD46 | repA101ts, oriR101, exo, bet, gam, tL3, PBAD, araC, and bla | AY048746 | 76 | |
| pT-ispA-STS | pTrc99A 벡터; lacIq; Ampr; E. coli유래 FPP Synthase인 ispA; Clausena lansium유래 santalene synthase 인 STS를 포함하는 Ptrc 발현벡터 | - | 77 | |
| pTAS-NA | pTrc99A 벡터; lacIq; Ampr; E. coli유래 FPP Synthase인 ispA 및 idi; Clausena lansium유래 santalene synthase 인 STS; E. faecalis 유래 mvaE 및 mvaS; S.pneumonia 유래 mvaK1, mvaK2 및 mvaD 포함하는 Ptrc 발현벡터 | - | In this study | 78 |
| pSNAK | pSTV28 벡터; lacZ; Kmr; E. faecalis 유래 mvaE 및 mvaS; S.pneumonia 유래 mvaK1, mvaK2 및 mvaD; E. coli 유래 idi를포함하는 Plac 발현 벡터 | - | 79 | |
| pSNAK(-E) | pSTV28 벡터; lacZ; Kmr; E. faecalis 유래 mvaS; S.pneumonia 유래 mvaK1, mvaK2 및 mvaD; E. coli 유래 idi를 포함하는 Plac 발현 벡터 | - | In this study | 80 |
| pSNA(-E)free | pSTV28 벡터; lacZ; E. faecalis 유래 mvaS; S.pneumonia 유래 mvaK1, mvaK2 및 mvaD; E. coli 유래 idi를 포함하는 항생제 마커가 없은 벡터 | - | In this study | 81 |
| pTEFAmvaE | pTrc99A 벡터; lacIq; Ampr; E. faecalis 유래 mvaE를 포함하는 Ptrc 발현벡터 | - | 82 | |
| pT-DHBSRYbbO | pTrc99A 벡터; lacIq; Ampr; P.agglomerans 유래 crtE, crtB 및 crtI; P.ananatis 유래의 crtY; 코돈최적화 된 uncultured marine bacterium 66A03 유래 SR; E.coli의 dxs 및 YbbO | - | 83 | |
| pT-HBSRYbbO | pTrc99A 벡터; lacIq; Ampr; P.agglomerans 유래 crtE, crtB 및 crtI; P.ananatis 유래의 crtY; 코돈최적화 된 uncultured marine bacterium 66A03 유래 SR; E.coli의 YbbO | - | In this study | 84 |
| pT-HBSREYbbO | pTrc99A 벡터; lacIq; Ampr; P.agglomerans 유래 crtE, crtB 및 crtI; P.ananatis 유래의 crtY; 코돈최적화 된 uncultured marine bacterium 66A03 유래 SR; E.coli의 YbbO; E. faecalis 유래 mvaE | - | In this study | 85 |
| pT-HBSREYbbOfree | pTrc99A 벡터; lacIq; P.agglomerans 유래 crtE, crtB 및 crtI; P.ananatis 유래의 crtY; 코돈최적화 된 uncultured marine bacterium 66A03 유래 SR; E.coli의 YbbO; E. faecalis 유래 mvaE를 포함하는 항생제 마커가 없은 벡터 | - | In this study | 86 |
| pT-dxr | pTrc99A 벡터; lacIq; Ampr; E.coli 유래의 dxr를 포함하는 Ptrc 발현벡터 | - | 87 | |
| pTAS-dxr | pTrc99A 벡터; lacIq; Ampr; E. coli유래 FPP Synthase인 ispA 및 dxr; Clausena lansium유래 santalene synthase 인 STS 포함하는 Ptrc 발현벡터 | - | In this study | 88 |
| pT-dxr/s | pTrc99A 벡터; lacIq; Ampr; E.coli 유래의 dxr 및 dxs를 포함하는 Ptrc 발현벡터 | - | 89 | |
| pT-ispA-MrBBS | pTrc99A 벡터; lacIq; Ampr; E. coli유래 FPP Synthase인 ispA; 코돈최적화 된 Matricaria recutita유래의 α-bisabolol synthase 인 MrBBS를 포함하는 Ptrc 발현벡터 | - | In this study | 90 |
| pTAS-dxs | pTrc99A 벡터; lacIq; Ampr; E. coli유래 FPP Synthase인 ispA 및 dxs; Clausena lansium유래 santalene synthase 인 STS 포함하는 Ptrc 발현벡터 | - | In this study | 91 |
| pTAB-idi-dxr | pTrc99A 벡터; lacIq; Ampr; E. coli유래 FPP Synthase인 ispA 및 dxr; 코돈최적화 된 Matricaria recutita유래의 α-bisabolol synthase 인 MrBBS를 포함하는 Ptrc 발현벡터 | - | In this study | 92 |
| pSNAK(-E)-dxs | pSTV28 벡터; lacZ; Kmr; E. faecalis 유래 mvaS; S.pneumonia 유래 mvaK1, mvaK2 및 mvaD; E. coli 유래 idi 및 dxs를 포함하는 Plac 발현 벡터 | - | In this study | 93 |
| pSNA(-E)-dxsfree | pSTV28 벡터; lacZ; E. faecalis 유래 mvaS; S.pneumonia 유래 mvaK1, mvaK2 및 mvaD; E. coli 유래 idi 및 dxs를 포함하는 항생제 마커가 없은 벡터 | - | In this study | 94 |
| pT-HBSREYbbOdxrfree | pTrc99A 벡터; lacIq; P.agglomerans 유래 crtE, crtB 및 crtI; P.ananatis 유래의 crtY; 코돈최적화 된 uncultured marine bacterium 66A03 유래 SR; E.coli의 YbbO 및 dxr; E. faecalis 유래 mvaE를 포함하는 항생제 마커가 없은 벡터 | - | In this study | 95 |
| pEGFP | pUC origin, lac promoter; EGFP, and AmpR | - | Clontech (US) | 96 |
| pEGFP-dxr | pUC origin, lac promoter; EGFP, E.coli의 dxr, and AmpR | - | In this study | 97 |
| pORI19 | Emr Ori+ RepA- lacZ' derivative of pORI28 | A System To Generate Chromosomal Mutations in Lactococcus lactis Which Allows Fast Analysis of Targeted Genes | - | 98 |
| pORI19-mvaA | pORI19-mvaA | - | In this study | 99 |
| pCI372 | E. coli/L. lactis shuttle vector, CamR | Identification of the minimal replicon of lactococcus lactis subsp. lactis UC317 plasmid pCI305 | - | 100 |
| pCIN | pCI372-FP promoter, MCS, rrnp terminator | - | In this study | 101 |
| pCIN-mvaA | pCIN-mvaA | - | In this study | 102 |
| pCIN-mvaA-EGFP | pCIN-mvaA,EGFP | - | In this study | 103 |
구축된 벡터는 모두 trc promoter를 사용하거나, trc promoter를 항시 발현할 수 있도록 변형하여 사용하였다. 따라서 구축된 pBFKC의 경우 trc promoter를 가지며, pTFKC(DPB)와 pTFCC(DPB)의 경우 항시 발현할 수 있도록 변형된 trc promoter를 가진다. 구축된 벡터의 multi-cloning site를 이용하여 원하는 경로를 삽입하였으며, 각각의 구축된 plasmid를 주형으로 대장균 삽입할 부분과 50 bp의 상동성을 가지는 프라이머를 이용하여 PCR을 수행하였다. 각 프라이머 정보는 표 4에 서열번호 3부터 서열번호 10으로 나타내었다. 얻어진 PCR 산물은 정제하여 pKD46을 포함한 대장균 MG1655(DE3) 수용성 세포(competent cell)에 1mm 간격의 큐벳을 통해 1.8 kV로 electro-transformation하고, 곧바로 SOC medium (2% Bacto Tryptone, 0.5% yeast extract, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl2, 10 mM MgSO4, 20 mM glucose) 1mL을 첨가하여, 37℃에서 1시간 동안 진탕 배양 한 후, 항생제를 첨가한 평판배지에 도말하여 37℃에서 배양 후 단일 콜로니를 얻었다. 여기서 얻은 단일 콜로니들을 소량의 증류수에 현탁하여 10분간 가열하고, 이를 원심 분리한 후 상층액을 주형으로 하여 서열번호 11부터 서열번호 18까지의 각 프라이머로 PCR을 수행하여 경로삽입 여부를 확인하였다. 항생제 마커 제거를 위해서는 외래경로 삽입이 확인된 균주에 pCP20 플라스미드를 형질전환하여 30℃에서 배양 후 단일 콜로니를 얻었으며, 여기서 얻은 단일 콜로니들을 주형으로 하여 상기 확인용 프라이머를 통해 PCR을 수행하고 항생제 마커가 제거된 것을 확인하였다. 확인된 콜로니는 43℃에 배양하여 온도민감성 플라스미드인 pCP20 플라스드를 제거하여 완성된 외래경로 삽입균주를 얻을 수 있었다.
2) MG1655(DE3) ΔadhE::MVAbottom 균주에서
dxr
,
dxs
유전자 결실
유전자 결실 또한 Datsenko와 Wanner의 방법을 이용하여 대장균 염색체 내 dxr 유전자를 결손하였다.
dxr 유전자를 상동성 재조합에 의하여 선별 마커 유전자인 카나마이신 유전자로 대체하기 위하여, pTFKC(DPB) 플라스미드의 카나마이신 유전자에 결합하면서 dxr 유전자의 상류 말단과 하류 말단의 염기 서열을 갖는 PCR 프라이머를 제조하였다. 정방향 프라이머는 dxr 유전자의 상류 말단의 50bp 염기서열 다음에, 카나마이신 유전자 결합 염기 서열 15bp로 구성되어 있으며, 역방향 프라이머는 dxr 유전자의 하류 말단의 50bp 염기서열 다음에, 카나마이신 유전자 결합 염기 서열 20bp로 구성되어 있다. 사용한 프라이머는 하기 표 7에 표시하였다. 서열번호 19과 서열번호 20의 올리고뉴클레오티드를 프라이머로 하고, pTFKC(DPB)을 주형으로 PCR 반응을 수행하였다.
상기의 정제된 PCR 반응산물은 pKD46 (Datsenko KA and Wanner BL, 2000 Proc Natl Acad Sci U.S.A., 97(12):6640-6645)을 포함한 MG1655(DE3)ΔadhE::MVAbottom 수용성 세포 (competent cell)로 형질전환하였다. 3.3mM mevalonate와 카나마이신을 포함한 평판 배지에서 콜로니를 얻고, 이 콜로니는 PCR을 통하여 카나마이신 유전자가 상동성 재조합에 의해서 dxr 유전자를 대체했는지를 확인할 수 있다. 또한 mevalonate가 없는 평판 배지에 도말하여 콜로니가 자라지 못하는 것으로 dxr 유전자의 결손에 따른 MEP 경로가 차단된 것을 확인할 수 있다. 콜로니 PCR에 사용된 프라이머는 대장균 MG1655(DE3) 염색체 상에서 dxr 유전자 바로 인접한 영역에 결합하도록 제조하였다. 사용한 확인용 프라이머는 서열번호 21, 22, 23의 올리고뉴클레오티드의 프라이머이다.
염색체 상의 항생제 마커 제거를 위해서는 외래경로 삽입이 확인된 균주에 pCP20 플라스미드를 형질전환하여 30℃에서 배양 후 단일 콜로니를 얻었으며, 여기서 얻은 단일 콜로니들을 주형으로 하여 확인용 프라이머를 통해 PCR을 수행하고 항생제 마커가 제거된 것을 확인하였다. 확인된 콜로니는 43℃에 배양하여 온도민감성 플라스미드인 pCP20 플라스미드를 제거하여 완성된 MG1655(DE3) Δdxr ΔadhE::MVAbottom 균주를 얻었다.
상기 방법과 동일한 방법으로 MG1655(DE3) Δdxr ΔadhE::MVAbottom 균주에서 dxs 결손을 진행하여 MG1655(DE3) Δdxr/s ΔadhE::MVAbottom 균주를 얻을 수 있다. 상동성 재조합을 위해 서열번호 24과 서열번호 25의 프라이머를 이용하여, pTFKC(DPB)을 주형으로 PCR 반응을 수행하였다. 이후 확인용 프라이머는 서열번호 26과 서열번호 27, 그리고 서열번호 28의 올리고뉴클레오티드를 이용하였다.
3. 플라스미드 구축 및 배양방법(MEP 경로 결손주)
- 염색체 내 외래 MVA 하부경로 삽입하고 MEP 상부경로 유전자를 결손시킨 균주 이용
- 상기 MEP 경로 결손주에 사용되는 플라스미드의 선별마커는 결손된 MEP 경로를 보완할 수 있는 활성화된 MVA 경로 또는 염색체의 결손된 MEP 경로 유전자가 될 수 있다. 상기 결손주에 도입할 수 있는 플라스미드의 수는 단일 또는 복수가 될 수 있으며, 단일 플라스미드 방식에서는 도입하는 단일 플라스미드에 의해 숙주의 MEP 경로 결손이 보완될 수 있어야 하고, 복수 플라스미드 방식에서는 도입하는 복수의 플라스미드가 모두 존재할 때에만 숙주의 MEP 경로 결손이 보완될 수 있도록 하는 것이다
1) 외래 MVA 경로 기반의 이소프레노이드 생합성 플라스미드
A. 단일 플라스미드 방식 (산탈렌 생산 예시)
- 숙주의 MEP 경로 결손을 보완하는 활성화된 MVA 경로가 단일 플라스미드에 모두 존재하는 방식으로 이 플라스미드에는 MVA 경로와 목적산물의 생합성 경로가 함께 존재하도록 구축한다.
i. 플라스미드 구축
상기 MEP 경로 결손주에서 외래 MVA 경로를 항생제 마커를 대체하는 선별마커로 사용하기 위하여, 산탈렌을 생산하는 플라스미드에 MVA 경로를 도입하여 재조합 플라스미드를 구축한다. 상기 결손주는 재조합 플라스미드가 도입될 때 생육이 가능하고 산탈렌이 생산된다.
산탈렌 생산 플라스미드인 pT-ispA-STS는 pTrc99A 벡터에 대장균(Escherichia coli)의 FPP synthase인 ispA 유전자와 Clausena lansium의 santalene synthase 유전자인 STS를 도입한 것이다. 상기 플라스미드에서 STS 유전자 뒤에 존재하는 제한효소부위 BglII와 SbfI을 이용해서 pSNAK 플라스미드에 있는 외래 MVA 경로 오페론을 클로닝하였다. pSNAK 플라스미드는 스트렙토코커스 뉴모니아(Streptococcus pneumoniae)의 mvaK1, mvaD 그리고 mvaK2와 엔테로코커스 패칼리스(Enterococcus faecalis)의 mvaE와 mvaS, 그리고 대장균(Escherichia coli)의 idi 유전자를 도입한 것이다. MVA 오페론은 양말단의 제한효소 BamHI과 SbfI으로 절단하여 pT-ispA-STS 벡터에 도입하였다. 제한효소 BamHI과 BglII는 동일한 외가닥 말단(cohesive end)을 지닌다. 최종적으로 산탈렌 생산 오페론과 MVA 경로 오페론이 함께 도입된 pTAS-NA를 구축하였다.
상기 플라스미드의 클로닝 과정에서 형질전환체의 선별은 대장균 DH5α 또는 대장균 MG1655(DE3) Δdxr ΔadhE::MVAbottom를 이용할 수 있다. 대장균 DH5α를 이용하는 경우에는 상기 구축하는 플라스미드에 존재하는 항생제 마커를 이용해서 앰피실린 플레이트에서 선별할 수 있다. 대장균 MG1655(DE3) Δdxr ΔadhE::MVAbottom를 이용하는 경우에는 숙주 대장균의 MEP 경로가 불활성화 되어서 상기에 구축하는 MVA 경로가 활성화 된 플라스미드가 도입되면 생육이 가능하기 때문에 항생제 없이도 형질전환체의 선별이 가능하다.
ii. 플라스미드 도입
상기 결손주는 재조합 플라스미드가 도입될 때 생육이 가능하고 산탈렌이 생산된다. 상기 구축된 플라스미드 pTAS-NA를 MEP 경로가 결손된 MG1655(DE3) Δdxr ΔadhE::MVAbottom 균주에 도입하여 mevalonate가 없는 플레이트에서 형질전환체를 얻음으로써, 플라스미드의 외래 MVA경로를 이용하는 재조합 균주를 선별할 수 있다. 이후 무항생제의 배지에서 배양하여 산탈렌의 생산성을 확인함으로써, MVA 경로 기반의 선별마커를 지니는 플라스미드의 유지 및 활성 능력을 확인 할 수 있다.
상기 항생제 프리 산탈렌 생산 관련 플라스미드를 갖는 재조합 균주를 무항생제 조건에서 종균배양하고, 생산배지인 2% (v/v) 글리세롤 및 0.2mM IPTG를 함유한 2YT배지(리터 당 16g 트립톤, 10g 이스트 추출물, 및 5g NaCl) 4mL와 데칸 1mL의 혼합 배지 중에서 배양한다. 배양은 길이 15 cm, 지름 25 mm의 홈이 있는 시험관에 상기 혼합 배지를 넣고 각 균주를 접종한 후, 진탕 배양기 (shaking incubator)에 약 250 rpm으로 교반하면서 30℃에서 배양한다.
iii.
배양 결과
야생형 대장균 MG155(DE3) 균주를 대조군으로 하여 새롭게 구축한 재조합 균주인 MG1655(DE3) Δdxr ΔadhE::MVAbottom에서의 산탈렌 생산량을 비교하였다. 배양 결과는 도 8과 표 3에 나타내었다.
상세히 설명하자면, MG1655(DE3)와 MG1655(DE3) ΔadhE::MVAbottom 균주에서는 항생제를 첨가하여 배양하였으며, 항생제 프리 시스템을 적용할 수 있는 MG1655(DE3) Δdxr ΔadhE::MVAbottom균주는 항생제 첨가 유무에 변화를 주어 배양을 진행하였다. 48시간의 배양 후 데칸층을 회수하여 GC를 통하여 산탈렌 생산량을 분석하였다. 그 결과, 79.8mg/L의 산탈렌을 생산한 MG1655(DE3)균주에 비하여 신규 재조합 균주인 MG1655(DE3) ΔadhE::MVAbottom 균주에서 5배 이상 높은 산탈렌 생산성(420.2mg/L)을 나타내었다. 또한, 무항생제 배양을 한 경우는 항생제를 첨가하였을 때 보다 더 높은 산탈렌 생산성(646.7mg/L)을 보였다. 이러한 산탈렌 생산량의 증가가 염색체에 추가로 도입된 MVA 하부경로에서 기인하였을 가능성을 확인하기 위하여 MG1655(DE3) ΔadhE::MVAbottom균주에서 같은 실험을 진행하였지만, 이 경우 오히려 산텔렌을 생산하지 못하는 양상을 나타내었다.
| 균주 (48h) | MG1655(DE3) | MG1655(DE3) ΔadhE::MVAbottom | MG1655(DE3) Δdxr  |
|
| pTAS-NA | pTAS-NA | pTAS-NA | ||
| 항생제 첨가 | + | + | + | - |
| 균체 성장량 (OD600nm) | 10.9 ± 0.5 | 9.1 ± 1.0 | 10.7 ± 0.3 | 12.7 ± 0.3 |
| 산탈렌 (mg/L) | 79.8 ± 0.9 | 2.9 ± 1.3 | 420.2 ± 69.3 | 646.7 ± 6.6 |
B. 복수 플라스미드 방식 (레티노이드 생산 예시)
- 숙주의 MEP 경로 결손을 보완하는 MVA 경로가 복수의 플라스미드들에 분산되어 존재하는 방식으로 이들 복수의 플라스미드가 모두 존재하는 경우에만 MVA 경로가 활성화 될 수 있도록 하는 것이다. 즉 이들 플라스미드들에는 MVA 경로와 목적산물의 생합성 경로가 각각의 플라스미드의 크기와 구성 유전자들의 필요한 발현량을 고려해서 효율적으로 분산 배치되도록 구축한다.
i. 플라스미드 구축
MVA 하부경로가 염색체에 도입된 결손주에서 사용할 수 있는 외래 MVA경로 기반의 플라스미드 선별마커는 MVA 상부경로 유전자인 mvaE 유전자와 mvaS 유전자가 있다. mvaE 유전자는 atoB 유전자와 mvaA 유전자가 융합된 유전자로 필요 시 상기 두 유전자로 나누어 선별마커로 사용할 수 있다. 이번 실험에서는 외래 MVA 경로를 발현하는 pSNAK 플라스미드와 레티노이드를 생산하는 pT-DHBSRYbbO를 이용하여 두 플라스미드를 무항생제 조건에서 유지하는 플라스미드 시스템을 구축한다. 선별마커인 mvaE 유전자와 mvaS 유전자를 두 플라스미드에 분산 배치하여 두 플라스미드가 함께 도입되어야 숙주의 MEP 경로 결손이 보완되도록 한다. MVA 경로 선별마커 유전자 하나를 비교적 낮은 복제개수(copy number)와 lac 프로모터를 지니는 pSNAK 플라스미드에서 높은 복제개수와 trc 프로모터를 이용하는 pT-DHBSRYbbO 플라스미드로 옮겨야 하므로, 더 높은 발현량을 요구하는 mvaE 유전자를 옮겼다. 즉 mvaS 유전자가 mvaE를 제외한 MVA 경로를 발현하는 플라스미드의 선별마커로 사용되고, mvaE 유전자가 레티노이드 생산 플라스미드의 선별마커로 작용한다.
pSNAK 플라스미드의 제한효소부위 HpaI을 이용하여 mvaE 유전자를 절단 후 self-ligation으로 mvaE 유전자가 제거된 pSNAK(-E) 플라스미드를 구축했다. pSNAK(-E) 플라스미드에서 5’말단에 인산기를 지니는 서열번호 29와 서열번호 30의 프라이머를 이용한 PCR을 통해 Kanamycin 항생제 마커 유전자를 제거한 후 self-ligation으로 pSNA(-E)free를 구축했다.
pT-DHBSRYbbO는 pTrc99A 벡터에 판토에아 아글루메란스(Pantoea agglomerans) 유래의 crtE, crtB 및 crtI, 판토에아 아나나티스(Pantoea ananatis) 유래의 crtY, 대장균의 dxs 및 YbbO, 그리고 코돈최적화 된 uncultured marine bacterium 66A03 유래의 SR 유전자가 도입된 플라스미드이다. 상기 플라스미드에서 5’말단에 인산기를 지니는 서열번호 31과 32의 프라이머를 이용한 PCR을 통해 dxs 유전자를 제거하여 pT-HBSRYbbO를 구축했다.
pTEFAmvaE 플라스미드는 pTrc99A 벡터에 엔테로코커스 패칼리스(Enterococcus faecalis)의 mvaE 유전자를 도입하여 제작된 플라스미드로 이를 주형으로 하여, 서열번호 33와 34의 프라이머을 이용한 PCR을 통해 trc 프로모터와 mvaE 유전자를 함께 증폭한다. 이를 제한효소 NotI과 SalI으로 절단하여 pT-HBSRYbbO 벡터의 동일 제한효소 부위로 삽입해서, idi 유전자(ipiHP1)와 crtY 유전자 사이에 위치시킨 플라스미드 pT-HBSREYbbO를 제작했다. pT-HBSREYbbO에서 서열번호 35와 36, 그리고 서열번호 37과 38의 프라이머를 이용한 두 번의 PCR을 통해 암피실린 항생제 마커를 제거하면서 HindⅢ 제한효소 사이트를 도입한 pT-HBSREYbbOfree를 구축했다.
ii.
플라스미드 도입 및 배양 결과
① 항생제 마커를 제거하지 않은 두개의 신규 플라스미드 pSNAK(-E)와 pT-HBSREYbbO를 MG1655(DE3) Δdxr ΔadhE::MVAbottom 균주에 함께 도입하여, 무항생제 배지에서 플라스미드를 유지할 수 있는 신규 재조합 균주를 구축하였다. 기존의 pSNAK와 pT-DHBSRYbbO 플라스미드를 지니는 MG1655(DE3) 균주를 대조군으로 하여 신규 재조합 균주를 항생제 첨가 유무에 따른 레티놀 생산성을 확인하였다.
배양 결과는 표 4와 도 9에 나타내었다. 상세히 설명하면, 기존의 MG1655(DE3) 균주보다 신규 재조합 균주인 MG1655(DE3) Δdxr ΔadhE::MVAbottom에서 레티노이드의 생산성이 증가하였음을 알 수 있다. 신규 균주의 항생제 유무에 따른 배양 결과를 비교해보면, 48시간의 배양시간 동안 무항생제 조건에서 균체 생장량이 항생제 조건에 비해서 약간 낮았지만 레티노이드의 생산성은 더 높았다. 이것으로 항생제의 선택압력 없이 플라스미드가 소실되지 않고 잘 유지 및 작동을 하고 있다는 것을 알 수 있으며, 대사흐름도 균체 생장에서 레티노이드 생산 쪽으로 더 많이 유도되고 있음을 알 수 있다. 또한 항생제 유무에 따른 레티노이드의 생산량 차이는 플라스미드에서 발현되는 항생제 저항성 유전자가 항생제의 독성을 완전히 보완하지 못한다는 것을 알 수 있다. 이번 배양에서 사용한 항생제는 암피실린과 카나마이신으로 암피실린은 세포벽의 합성을 저해하는 작용을 하고, 카나마이신은 리보솜의 30s 소단위체를 저해하여 전반적인 단백질 생산을 감소시키는 역할을 한다. 따라서 무항생제 조건의 배양에서는 상기 세포에 악영향을 미치는 인자의 부재로 단백질 합성이 보다 잘 되어 생산량이 증가 하였다고 판단된다.
| 균주 (48h) | MG1655(DE3) | MG1655(DE3) Δdxr ΔadhE::MVAbottom | |
| pSANK / pT-DHBSRYbbO | pSNAK(-E) / pT-HBSREYbbO | ||
| 항생제 첨가 | + | + | - |
| 균체 성장량 (OD600nm) | 18.3 ± 0.5 | 11.4 ± 0.1 | 11.5 ± 0.2 |
| 총 레티노이드 (mg/L) | 75.4 ± 0.1 | 83.7 ± 2 | 108 ± 1 |
② 또한 항생제 저항성 유전자들이 제거된 플라스미드들인 pSNA(-E)free와 pT-HBSREYbbOfree를 함께 MG1655(DE3) Δdxr ΔadhE::MVAbottom 균주에 형질 전환하여 무항생제 조건에서 배양을 진행하였다. 배양은 상기 레티노이드 배양조건으로 진행하였으며, 대장균의 테스트 튜브 배양 최대 시간인 72시간까지 진행하였다.
배양 결과는 표 5와 도 10에 나타내었다. 상세히 설명하면, 항생제 마커를 완전히 제거한 플라스미드가 도입된 재조합 균주에서의 레티노이드 생산속도가 가장 빠르고, 48시간에 최대 생산성을 보였으며 72시간에는 탄소원 고갈의 영향으로 약간 감소하는 것을 볼 수 있다. 따라서 플라스미드에서 항생제 마커를 제거함으로써 숙주세포의 대사적인 부담이 감소하는 것으로 추정된다.
| 균주 (72h) | MG1655(DE3) | MG1655(DE3) Δdxr ΔadhE::MVAbottom | |
| pSANK / pT-DHBSRYbbO | pSNAK(-E) / pT-HBSREYbbO | pSNA(-E)free / pT-HBSREYbbOfree | |
| 항생제 첨가 | + | - | - |
| 균체 성장량 (OD600nm) | 22.1 ± 0.03 | 12.9 ± 0.08 | 13.0 ± 0.4 |
| 총 레티노이드 (mg/L) | 173.3 ± 6 | 218.1 ± 1 | 204.2 ± 3 |
2) 염색체의 결손된 MEP 경로 유전자를 선별 마커로 한 MEP 경로 기반의 이소프레노이드 생합성 플라스미드
A. 단일 플라스미드 방식 (산탈렌 생산 예시)
- 염색체의 결손된 MEP 경로 유전자를 선별 마커로 갖는 단일 플라스미드를 구축하고 숙주에 도입하는 방식으로, 이 경우에 숙주의 MEP 경로 결손은 도입된 플라스미드에 의해 보완되게 된다.
i. 플라스미드 구축
산탈렌 생산 플라스미드인 pT-ispA-STS의 산탈렌 생합성 오페론 뒤에 상기 결손주에서 결손된 MEP 경로 유전자인 dxr을 도입하여 pTAS-dxr를 구축하였다. 상세히 설명하자면, pTrc99A 플라스미드에 대장균의 dxr 유전자가 도입되어 있는 pT-dxr 플라스미드를 주형으로 하여 서열번호 39과 서열번호 40의 프라이머를 이용한 PCR을 통해 dxr 유전자의 양말단에 제한효소부위 BglII과 XhoI를 도입하면서 증폭하였다. 이를 pT-ispA-STS벡터의 STS 유전자 뒤에 존재하는 동일 제한효소부위 BglII와 SalI을 이용한 클로닝을 통해 결손된 dxr 유전자를 지니는 산탈렌 생산 플라스미드 pTAS-dxr을 구축하였다. 제한효소 SalI과 XhoI은 동일한 외가닥 말단(cohesive end)을 지닌다.
상기 플라스미드의 클로닝 과정에서 형질전환체의 선별은 대장균 DH5α 또는 대장균 MG1655(DE3) Δdxr ΔadhE::MVAbottom를 이용할 수 있다. 대장균 DH5α를 이용하는 경우에는 상기 구축하는 플라스미드에 존재하는 항생제 마커를 이용해서 앰피실린 플레이트에서 선별할 수 있다. 대장균 MG1655(DE3) Δdxr ΔadhE::MVAbottom를 이용하는 경우에는 숙주 대장균의 MEP 경로가 불활성화 되어서 상기에 구축하는 dxr 유전자가 도입된 플라스미드가 도입되면 생육이 가능하기 때문에 항생제 없이도 형질전환체의 선별이 가능하다.
ii. 플라스미드 도입
상기 구축된 플라스미드 pTAS-dxr을 MEP 경로가 차단되어 있는 MG1655(DE3) Δdxr ΔadhE::MVAbottom 균주에 도입하여 Mevalonate가 없는 플레이트에서 균주를 얻음으로써, 플라스미드의 dxr 유전자를 이용하여 내재 MEP 경로를 이용하는 균주를 선별할 수 있다. 이후 무항생제의 배지에서 배양을 진행하여 산탈렌을 생산성을 확인함으로써, 결손된 MEP 경로 유전자를 이용한 플라스미드의 유지 및 활성을 확인 할 수 있다.
상기 항생제 불필요 산탈렌 생산 플라스미드를 갖는 재조합 균주를 무항생제 조건에서 종균배양하고, 생산배지인 2% (v/v) 글리세롤 및 0.2mM IPTG를 함유한 2YT 배지(리터 당 16g 트립톤, 10g 이스트 추출물, 및 5g NaCl) 4mL와 데칸 1mL의 혼합 배지 중에서 배양한다. 배양은 길이 15 cm, 지름 25 mm의 홈이 있는 시험관에 상기 혼합 배지를 넣고 각 균주를 접종한 후, 진탕 배양기 (shaking incubator)에 약 250 rpm으로 교반하면서 30℃에서 배양한다.
iii.
배양 결과
야생형 대장균 MG155(DE3) 균주를 대조군으로 하여 신규 규축 재조합 균주인 MG1655(DE3) Δdxr ΔadhE::MVAbottom에서의 산탈렌 생산량을 비교하였다. 배양 결과는 도 11과 표 6에 나타내었다. 상세히 설명하자면, MG1655(DE3)와 MG1655(DE3) 
adhE::MVAbottom 균주에서는 항생제를 첨가하여 배양하였으며, 항생제 프리 시스템을 적용할 수 있는 MG1655(DE3) Δdxr ΔadhE::MVAbottom균주는 항생제 첨가 유무에 변화를 주어 배양을 진행하였다. 48시간의 배양 후 데칸층을 회수하여 GC를 통하여 산탈렌 생산량을 분석하였다. 그 결과 MG1655(DE3)의 경우 0.6mg/L의 산탈렌을 생산한 반면, 신규구축한 MG1655(DE3) Δdxr ΔadhE::MVAbottom균주는 4.5mg/L의 산탈렌을 생산하였다. MG1655(DE3) ΔadhE::MVAbottom균주에서의 산탈렌 생산량 또한 3.4mg/L로 야생형 균주에 비하여 많은 양의 산탈렌을 생산하였다. 증가한 산탈렌의 양이 MVA 하부경로의 추가도입에서 기인하였을 가능성도 있지만, 메발로네이트를 추가 도입해주지 않았기 때문에 MVA 하부경로를 통한 IPP와 DMAPP의 증가로 보기 힘들다. adhE가 결손되면서 Acetyl-CoA가 부산물인 에탄올을 생산하지 않으면서 축적되게 되고, 이로 인해 Glucose의 해당과정의 흐름이 변하여 MEP경로의 전구물질인 G3P와 Pyruvate의 양이 증가하였을 수 있다. 따라서 산탈렌의 생산량이 증가하였을 가능성이 있다.
| 균주 (48h) | MG1655(DE3) | MG1655(DE3) ΔadhE::MVAbottom | MG1655(DE3) Δdxr ΔadhE::MVAbottom | |
| pTAS-dxr | pTAS-dxr | pTAS-dxr | ||
| 항생제 첨가 | + | + | + | - |
| 균체 성장량 (OD600nm) | 4.9 ± 0.1 | 5.5 ± 0.2 | 5.0 ± 0.2 | 4.8 ± 0.2 |
| 산탈렌 (mg/L) | 0.6 ± 0.0 | 3.4 ± 0.1 | 4.5 ± 0.2 | 3.9 ± 0.1 |
B. 복수 플라스미드 방식 (산탈렌 + 비사볼롤 생산 예시)
- 염색체의 결손된 MEP 경로 유전자들을 선별 마커들로 갖는 복수의 플라스미드를 구축하고 숙주에 도입하는 방식으로, 이 경우에 숙주의 MEP 경로 결손은 복수의 플라스미드가 모두 존재하는 경우에만 보완되도록 결손된 MEP 경로 유전자들이 각각의 플라스미드에 고르게 분산되도록 한다. 특히 숙주로 사용하는 결손주에서 염색체의 MEP 경로 유전자들은 도입하는 플라스미드의 숫자 이상으로 결손이 되어야 한다. 즉, 2개의 플라스미드를 사용하는 경우에는 최소한 2개 이상의 염색체의 MEP 경로 유전자들이 결손되어야 하고 이들 유전자들은 선별마커로 각각의 플라스미드에 분산배치 되게 된다.
i. 플라스미드 구축
산탈렌 생산 플라스미드인 pT-ispA-STS의 산탈렌 생합성 오페론 뒤에 dxs 유전자를 도입하여 pTAS-dxs를 구축하였다. 상세히 설명하자면, pTrc99A 벡터에 대장균의 dxs 유전자와 dxr 유전자가 도입된 pT-dxs/r 플라스미드를 주형으로 하여 서열번호 41과 서열번호 42의 프라이머를 이용한 PCR을 통해 dxs 유전자의 양말단에 제한효소부위 BglII와 XhoI을 도입하면서 증폭하였다. 상기 PCR 산물과 pT-ispA-STS벡터의 STS 유전자 뒤에 존재하는 동일 제한효소부위 BglII와 SalI을 이용한 클로닝을 통해 dxs 유전자를 지니는 산탈렌 생산 플라스미드 pTAS-dxs를 구축하였다. 제한효소 SalI과 XhoI은 동일한 외가닥 말단(cohesive end)을 지닌다.
마찬가지로 pTrc99A 벡터에 대장균(Escherichia coli)의 FPP Synthase인 ispA 유전자와 Matricaria recutita 의 α-bisabolol synthase 유전자인 MrBBS로 구성된 비사볼롤 생산 플라스미드인 pT-ispA-MrBBS의 비사볼롤 생합성 오페론 뒤에 dxr 유전자와 idi 유전자(b2889)를 도입하여 pTAB-idi-dxr를 구축하였다. idi 유전자는 IPP 이소머라제로 상기 구축한 플라스미드에 의하여 MEP경로가 강화되었을때, IPP와 DMAPP의 균형을 맞추어 주어 이소프레노이드의 생산성을 향상시킬 수 있다. 구축과정을 상세히 설명하자면 pT-ispA-MrBBS벡터의 MrBBS 유전자 뒤에 존재하는 제한효소부위 BglII와 SalI을 이용하여, 서열번호 43과 서열번호 44의 프라이머를 이용한 PCR을 통해 제한효소부위 BamHI과 SalI을 도입하면서 증폭한 idi 유전자와 서열번호 45과 서열번호 46의 프라이머를 이용한 PCR을 통해 제한효소부위 SalI과 HindIII를 도입하면서 증폭한 dxr 유전자를 비사볼롤 생산 플라스미드에 도입하여 pTAB-idi-dxr를 구축하였다.
상기 플라스미드의 클로닝 과정에서 형질전환체의 선별은 대장균 MG1655(DE3) Δdxr/s ΔadhE::MVAbottom를 이용할 수 있다. 대장균 MG1655(DE3) Δdxr/s ΔadhE::MVAbottom를 이용하는 경우에는 숙주 대장균의 MEP 경로가 불활성화 되어서 상기에 구축하는 dxr 유전자와 dxs 유전자가 각각 도입된 두 플라스미드가 함께 도입될 때 생육이 가능하기 때문에 항생제 없이도 형질전환체의 선별이 가능하다.
ii. 플라스미드 도입
상기 구축한 dxs 유전자를 선별마커로 지니는 플라스미드 pTAS-dxs와 dxr 유전자를 선별마커로 지니는 플라스미드 pTAB-idi-dxr를 dxr와 dxs유전자가 모두 결손되어 있는 MG1655(DE3) Δdxr/s ΔadhE::MVAbottom 균주에 함께 형질 전환하여 재조합 균주를 구축한다. 상기 재조합 균주를 무항생제 배지환경에서 배양하여 산탈렌과 비사볼롤의 생산을 확인함으로써 결손된 MEP 유전자들 각각이 복수의 플라스미드들의 선별 마커로 작동함을 확인 할 수 있다.
상기 복수 플라스미드를 갖는 재조합 균주를 무항생제 조건에서 종균배양하고, 생산배지인 2% (v/v) 글리세롤 및 0.2mM IPTG를 함유한 2YT 배지(리터 당 16g 트립톤, 10g 이스트 추출물, 및 5g NaCl) 4mL와 데칸 1mL의 혼합 배지 중에서 배양한다. 배양은 길이 15 cm, 지름 25 mm의 홈이 있는 시험관에 상기 혼합 배지를 넣고 각 균주를 접종한 후, 진탕 배양기 (shaking incubator)에 약 250 rpm으로 교반하면서 30℃에서 배양한다.
iii.
배양 결과
상기 구축한 재조합 균주를 항생제 첨가 유무에 따른 배지를 이용하여 배양을 진행하였다. 58시간의 배양 후 데칸층을 회수하여 GC를 통하여 산탈렌과 비사볼롤 생산량을 분석하였다. 배양 결과는 도 12와 표 7에 나타내었다. 상세히 설명하자면 항생제를 첨가하지 않은 배지에서도 산탈렌과 비사볼롤이 각 7.7mg/L, 40.7mg/L로 생산되었다. 이를 통해 산탈렌 생합성 유전자와 비사볼롤 생합성 유전자를 각각 지니고 있는 두 플라스미드가 모두 잘 유지되고 유전자를 발현하고 있다는 것을 알 수 있다.
| 균주 (58h) | MG1655(DE3) Δdxs/r ΔadhE::MVAbottom | |
| pTAS-dxs / pTAB-idi-dxr | ||
| 항생제 첨가 | + | - |
| 균체 성장량 (OD600nm) | 10.1 ± 0.8 | 10.7 ± 0.7 |
| 산탈렌 (mg/L) | 14.3 ± 1.6 | 7.7 ± 2.5 |
| 비사볼롤 (mg/L) | 49.7 ± 5.0 | 40.7 ± 7.1 |
C. 외래 MVA 경로 추가 방식 (레티노이드 생산 예시)
- 이소프레노이드 생산에 외래 MVA 경로를 도입하는 것이 유리하다는 선행결과를 통해서 외래 MVA 경로를 플라스미드에 도입하면서도 MEP 경로 결손 유전자를 선별마커로 사용하는 방법을 제공한다. 이 경우에 숙주 균주는 MEP 경로와 MVA 경로가 모두 활성화 되기 때문에 높은 이소프레노이드 생산성을 기대할 수 있다. 다만 복수의 플라스미드를 사용하는 경우에 추가로 도입되는 외래 MVA 경로가 어느 한쪽에 집중되지 않고 사용하는 플라스미드들에 고르게 분산되도록 해서 모든 플라스미드들이 존재할 때에만 숙주의 결손된 MEP 경로가 보완이 되도록 한다.
i.
플라스미드 구축
상기 구축한 MVA 경로 유전자를 두 플라스미드에 나누어 발현하는 무항생제 레티노이드 생산 균주를 이용하여 MEP 경로의 결손 유전자를 선별마커로 사용하면서 외래 MVA경로도 함께 이용하는 재조합 플라스미드를 구축한다. 플라스미드의 사이즈가 큰 상기 mvaE 유전자가 도입된 레티노이드 생산 플라스미드 pT-HBSREYbbOfree에는 비교적 사이즈가 작은 dxr 유전자를 도입하고, 사이즈에 여유가 있는 mvaE 유전자를 제외한 MVA 경로를 발현하는 플라스미드 pSNAK(-E)에는 dxs 유전자를 도입한다.
pT-dxs/r 플라스미드를 주형으로 하여 서열번호 47과 서열번호 48의 프라이머를 이용한 PCR을 통해 MEP 상부경로 유전자인 dxs를 양 말단에 BglII와 XhoI을 도입하면서 증폭한다. pSNAK(-E)를 주형으로 하여 서열번호 49와 서열번호 50의 프라이머를 이용한 PCR로 양 말단에 BglII와 XhoI 제한효소서열을 도입하면서 벡터를 증폭한다. 두 PCR 산물을 제한효소 BglII와 XhoI으로 절단하여 벡터의 idi 유전자(b2889)와 mvaS 유전자 사이에 dxs 유전자를 삽입하여 pSNAK(-E)-dxs 구축한다. 이후 항생제 제거 프라이머를 이용한 PCR을 통해 카나마이신 항생제 저항성 유전자를 제거하여 pSNA(-E)-dxsfree를 구축한다.
pT-dxr 플라스미드를 주형으로 하여 서열번호 51과 서열번호 52의 프라이머를 이용한 PCR을 통해 MEP 상부경로 유전자인 dxr의 양 말단에 제한효소 NheI과 ScaI을 도입하면서 증폭하고, pT-HBSREYbbOfree 플라스미드를 서열번호 36과 서열번호 53, 그리고 서열번호 37과 서열번호 54의 프라이머를 이용한 두 번의 PCR을 통해 제한효소 XhoI 부위를 기점으로 각 7.1kb, 5.6kb의 두 조각으로 증폭한다. 두 조각은 xhoI으로 다시 연결하고, dxr 유전자는 제한효소 ScaI과 NheI으로 절단하여 레티노이드 오페론의 말단에 삽입하여 pT-HBSREYbbOdxrfree를 구축한다.
ii.
플라스미드 도입
MEP 경로의 dxr 유전자와 MVA 경로의 mvaE 유전자를 지니는 pT-HBSREYbbOdxrfree 플라스미드와 MEP 경로의 dxs 유전자와 mvaE 유전자를 제외한 모든 MVA 경로 유전자를 지니는 pSNA(-E)-dxsfree를 대장균 MG1655(DE3) Δdxr/s ΔadhE::MVAbottom에 함께 도입하여 MVA경로와 MEP경로를 동시에 이용하는 무항생제 레티놀 생산 재조합 균주의 레티놀 생산성을 확인한다.
iii.
배양 결과
MEP 경로 결손주에서 외래 MVA 경로만 이용하는 무항생제 레티놀 생산 재조합 균주와 함께 상기 구축된 MVA 경로와 MEP 경로를 동시에 이용하는 무항생제 레티놀 생산 재조합 균주를 무항생제 조건에서 배양하여 레티놀 생산성을 비교하였다. 배양 결과는 표 8과 도 13에 나타내었다. 상세히 설명하면, 외래 MVA 경로만으로 MEP 경로 결손을 보완하였던 레티노이드 생산 균규와 비교하여, 상기 구죽한 MVA 경로와 MEP 경로를 동시에 이용하는 무항생제 레티놀 생산 재조합 균주가 항생제 저항성 유전자를 제거 하였을 때, 보다 높은 레티놀 생산성을 나타내었다. 이를 통해 외래 MVA 경로를 추가적으로 보완하면서, 결손된 MEP 경로 유전자를 선별마커로 이용할 수 있다는 것을 확인하였다.
| 균주 (72h) | MG1655(DE3) Δdxr ΔadhE::MVAbottom | MG1655(DE3) Δdxs/r ΔadhE::MVAbottom | |
| pSNA(-E)free / pT-HBSREYbbOfree | pSNAdxs(-A) / pT-HBSRAYbbOdxr | pSNAdxs(-A)free / pT-HBSRAYbbOdxrfree | |
| 항생제 첨가 | - | - | - |
| 균체 성장량 (OD600nm) | 12.7 ± 0.24 | 12.6 ± 0.28 | 12.1 ± 0.12 |
| 총 레티노이드 (mg/L) | 153.2 ± 2 | 124.0 ± 3 | 207.2 ± 1 |
3) 염색체의 결손된 MEP 경로 유전자를 선별 마커로 한 단백질 발현용 플라스미드
- GFP단백질을 이용하여 단백질 발현의 안정성 확인
- 단일 단백질 발현에만 국한 되는 것이 아니라 다양한 대사산물 생합성 경로 발현에도 이용할 수 있다.
i. 플라스미드 구축
녹색 형광을 발현하는 벡터 pEGFP의 EGFP 유전자 뒤에 결손주의 결손된 MEP 경로 유전자인 dxr을 도입하여 pEGFP-dxr을 구축하였다. 상세히 설명하자면, 상기 벡터의 EGFP 유전자 뒤의 제한효소부위 StuI과 SpeI 사이트를 이용하여, 서열번호 51과 서열번호 52의 프라이머를 이용한 PCR을 통해 증폭한 dxr 유전자를 도입해 주었다. 증폭된 PCR 산물은 양 말단에 제한효소부위 ScaI과 NheI이 도입되었다. 제한효소 StuI과 제한효소 ScaI은 평활말단(blunt end)를 지니며, 제한효소 SpeI과 NheI은 동일한 외가닥 말단(cohesive end)를 지닌다. 최종적으로 dxr 유전자를 지니며 녹색형광을 발현하는 플라스미드인 pEGFP-dxr을 구축한다.
ii. 플라스미드 도입
상기 구축된 pEGFP-dxr 플라스미드를 MG1655(DE3) Δdxr ΔadhE::MVAbottom 균주에 형질전환한다. 이를 항생제를 포함하지 않은 LB 평판배지에 도말하여 형광을 관찰한다.
상기 구축된 재조합 균주의 목표 단백질의 발현을 확실히 하기 위하여, 유도물질인 IPTG를 1mM 넣어주어 배양을 진행한다. 상세히 설명하자면 균주를 무항생제 조건에서 종균배양하고, 생산배지인 LB 배지 5ml에 OD600nm 0.1이 되도록 접종하여 배양한다. 배양 후 OD600nm 0.6이 되었을 시점에 1mM IPTG를 첨가해준다. 배양은 길이 15 cm, 지름 25 mm의 홈이 있는 시험관에 상기 혼합 배지를 넣고 각 균주를 접종한 후, 진탕 배양기 (shaking incubator)에 약 250 rpm으로 교반하면서 30℃에서 배양한다. 이 배양액을 채취하여 무항생제 배지 조건에서 플라스미드에 함유된 유전자의 발현의 안정성을 SDS-PAGE와 형광측정을 통하여 확인한다.
iii.
배양 결과
SDS-PAGE와 형광발현 결과는 도 14에 나타내었다. 상세히 설명하자면, 형광현미경을 통하여 녹색 형광을 관찰한 결과 항생제를 선별마커로 지니는 대조군보다 결손된 MEP경로 유전자를 플라스미드의 선별마커로 사용한 경우, 보다 선명한 형광을 나타내는 것을 확인하였다. 또한 SDS-PAGE를 통한 단백질 발현량을 비교해 보아도 대조군에 비하여 새롭게 구축한 균주의 무항생제 배양에서 강한 단백질 발현양을 나타내었다. IPTG와 같은 유도제를 처리 하지 않아도, 균주의 결손된 MEP경로 유전자의 선택압력에 의해서 플라스미드를 충분히 유지하고 유전자를 발현하고 있는 것을 확인하였다.
4. MVA 경로 변이 균주 구축 방법
1) 유산균 HMG-CoA reductase를 코딩하는 유전자(mvaA)의 불활성화
유산균의 내재하는 MVA 경로에서 율속제한효소(rate-limiting enzyme)인 Hydroxymethylglutaryl-CoA reductase (mvaA) 유전자를 Homologous recombination system을 이용하여 불활성화 하였다. Lactococcus lactis MG1363의 mvaA유전자 변이를 위해 우선 MG1363에서 mvaA 유전자 앞 1Kb 부분을 서열번호 55과 서열번호 56의 프라이머를 이용하여 중합효소연쇄반응(PCR)을 통해 증폭하고 mvaA유전자 뒤 1Kb 부분을 서열번호 57과 서열번호 58의 프라이머를 이용하여 PCR을 통해 증폭한 후 두 PCR 산물을 서열번호 59과 서열번호 60의 프라이머를 이용하여 Splicing by overhang extension(SOE) PCR을 통해 증폭하였다. 또한 pORI19 플라스미드를 서열번호 61과 서열번호 62의 프라이머를 이용하여 PCR을 통해 증폭한 후 두 PCR산물을 Ligation한 후 EC1000 수용성 세포(competent cell) 100ul에 2mm 간격의 큐벳을 통해 25 ㎌, 200Ω, 2,500V 조건에서 electroporation을 수행하였다. Electroporation한 후에 LB 배지 1ml을 첨가하고 37℃에서 30분간 배양 후 300ug/ml 에리트로마이신 항생제가 첨가된 LB 고체 평판배지에 100㎕ 도말하고 37℃에서 12시간 배양하여 pORI19-mvaA 플라스미드를 얻었다(표 2).
유산균의 수용성 세포(competent cell)를 제작하기위해 플라스미드 pVE6007를 함유한 L. lactis MG1363(pVE6007) 균주를 0.5% (v/v) glucose와 5ug/ml 클로람페니콜 항생제가 첨가된 5ml의 M17배지(1리터당 5.0g Pancreatic Digest of Casein, 5.0g Soy peptone, 5.0g Beef Extract, 2.5g Yeast Extract, 0.5g Ascorbic Acid, 0.25g Magnesium Sulfate, 10.0g Disodium-β-glycerophosphate)에 접종하고, 온도 30℃에서 16-24시간 종균배양한다. 0.5% (v/v) glucose, 0.5M sucrose, 1.5% (w/v) glycine, 5ug/ml 클로람페니콜이 첨가된 9ml의 M17 배지에 종균배양액 1ml을 접종 후 30℃에서 16-24시간 배양한다. 상기배양액 5ml을 동일한 새 배지 35ml에 접종하고 다시 30℃에서 OD600nm, 0.25까지 배양한다. 수용성 세포를 만들기 위해 상기 배양액을 얼음에서 5분간 냉각한 후에 4℃, 5,000rpm에서 15분간 원심분리해서 상층액을 제거하고, 배양액과 동일한 양의 40ml 세척완충액(0.5M sucrose, 10% glycerol)으로 2회 세척한다. 세척한 균체를 0.4ml의 세척완충액에 현탁해서 수용성 세포를 얻었다.
상기에서 얻은 수용성 세포 100ul에 pORI19-mvaA 플라스미드 3-5㎍을 2mm간격의 큐벳을 통해 25 ㎌, 200Ω, 2,500V 조건에서 electroporation을 수행한다. Electroporation한 후에 5㎍/ml 클로람페니콜이 첨가된 GM17배지 1ml을 첨가하고 30℃에서 2시간 배양 후 5㎍/ml 클로람페니콜과 5㎍/ml 에리트로마이신이 첨가된 GM17 고체 평판배지에 100㎕ 도말하여 MG1363(pORI19-mvaA, pVE6007)균주를 얻었다.
pVE6007 플라스미드 제거를 위해 상기에서 얻은 균주를 5㎍/ml 클로람페니콜과 5㎍/ml 에리트로마이신이 첨가된 5ml의 GM17 배지에 접종하여 30℃에서 16-24시간 배양 후 배양액 1ml을 cell down하여 GM17 배지 1ml로 세척 2번 진행한 후 5㎍/ml 에리트로마이신이 첨가된 10ml의 GM17 배지에 0.1% (v/v)으로 접종하여 30℃에서 16-24시간 배양한다. 동일한 배지에 0.1% (v/v)으로 접종하여 37℃에서 12시간 간격으로 계대배양을 3회 실시한 후 5ug/ml 에리트로마이신이 첨가된 GM17 고체 평판배지에 105-7 희석해 도말하여 30℃에서 16-24시간 배양한다.
Single cross over(SCO)가 일어난 균주를 PCR을 통해서 선별하였다. 200개의 콜로니를 서열번호 63와 서열번호 64의 프라이머와 함께 upstream 확인을 위한 서열번호 65 프라이머와 downstream 확인을 위한 서열번호 66 프라이머를 추가로 각각 넣어주어 총 3개의 프라이머를 이용하여 PCR을 진행하였다. Upstream에서 SCO가 일어난 경우 서열번호 63과 서열번호 65의 프라이머에 의해 확인이 가능하며 downstream에서 SCO가 일어난 경우 서열번호 64과 서열번호 66의 프라이머에 의해 확인이 가능하다. 그리고 SCO가 일어나지 않은 wild type의 경우 서열번호 63와 서열번호 64의 프라이머에 의해 확인이 가능하다. SCO가 일어난 균주를 5㎍/ml 에리트로마이신이 첨가된 5ml의 GM17 배지에 접종하여 30℃에서 16-24시간 배양 후 배양액 1ml을 cell down하여 GM17 배지 1ml로 세척 2번 진행한 후 3.3mM mevalonate가 첨가된 10ml의 GM17 배지에 0.1% (v/v)으로 접종하여 30℃에서 16-24시간 배양한다. 유전자 mvaA가 변이된 변이주의 생육을 위해서는 배지에 mevalonate를 첨가한다. 동일한 배지에 0.1% (v/v)으로 접종하여 30℃에서 12시간 간격으로 계대배양을 3회 이상 실시한 후 3.3mM mevalonate가 첨가된 GM17 고체 평판배지에 105-7 희석해 도말하여 30℃에서 16-24시간 배양한다.
Double cross over(DCO)가 일어난 균주를 PCR을 통해서 선별하였다. 400개의 콜로니를 서열번호 63와 서열번호 64의 프라이머를 이용하여 PCR을 진행하여 Lactococcus lactis MG1363ΔmvaA 균주를 구축하였다.
5. 플라스미드 구축 및 배양방법 (MVA 경로 변이주)
- 숙주의 염색체 내 MVA 상부경로 유전자를 변이시킨 균주 이용
1) 대장균-유산균 재조합 셔틀 벡터 구축
기존 대장균-유산균 셔틀벡터 pCI372를 제한효소 AgeI과 NheI으로 절단하고, promoter, multi-cloning site, terminator를 도입한 pCIN벡터를 구축하였다(표 2).
2) 염색체의 변이된 MVA 경로 유전자를 선별 마커로 한 단백질 발현용 플라스미드
i. 플라스미드 구축
선별마커로 이용하기 위한 mvaA 유전자를 MG1363 균주로부터 서열번호 67과 서열번호 68의 프라이머를 이용하여 중합효소연쇄반응(PCR)을 통해 증폭하고 제한효소 BamHI과 XbaI를 절단한 후에 pCIN벡터의 동일한 제한효소부위에 도입해서 pCIN-mvaA벡터를 구축하였다. 녹색 형광 단백질 발현확인을 위한 EGFP 유전자를 pEGFP벡터로부터 서열번호 69과 서열번호 70의 프라이머를 이용하여 중합효소연쇄반응(PCR)을 통해 증폭하고 제한효소 SalI과 SphI으로 절단한 후에 pCIN-mvaA벡터의 동일한 제한효소부위에 도입해서 pCIN-mvaA-EGFP를 구축하였다(도 10). 상기 구축한 벡터들은 표 2에 나타내었다.
ii. 플라스미드 도입
상기 pCIN-mvaA-EGFP 재조합 플라스미드를 L. lactis MG1363ΔmvaA 균주에 형질전환하여 mvaA를 선별마커로 갖는 유산균 형질전환체를 제작한다.
pCIN-mvaA-EGFP 재조합 셔틀벡터를 L. lactis MG1363ΔmvaA 균주에 형질전환하기 위하여 수용성 세포(competent cell)를 제작한다. MG1363ΔmvaA 균주를 0.5% (v/v) glucose와 3.3mM mevalonate가 첨가된 5ml의 M17 배지에 접종하고, 온도 30℃에서 16-24시간 종균배양한다. 0.5% (v/v) glucose, 0.5M sucrose, 1.5% (w/v) glycine, 3.3mM mevalonate가 첨가된 9ml M17 배지에 종균배양액 1ml을 접종 후 30℃에서 16-24시간 배양한다. 상기 배양액 5ml을 동일한 새 배지 35ml에 접종하고 다시 30℃에서 OD600, 0.25까지 배양한다. 수용성 세포를 만들기 위해 상기 배양액을 얼음에서 5분간 냉각한 후에 온도 4℃, 5000rpm에서 15분간 원심분리해서 상층액을 제거하고, 배양액과 동일한 양의 40ml의 세척완충액 (0.5M sucrose, 10% glycerol)으로 2회 세척한다. 세척한 균체를 0.4ml의 세척완충액에 현탁해서 수용성 세포를 얻는다.
상기 얻어진 수용성 세포 100ul에 pCIN-mvaA-EGFP 플라스미드를 5ul 넣고 섞은 뒤 혼합액을 electroporation cuvette (2mm 간격)에 넣고, 25 ㎌, 200Ω, 2,500V 조건에서 electroporation을 수행한다. Electroporation 한 후에 0.5% (v/v) glucose가 첨가된 M17 배지를 1ml 첨가하고 30℃에서 1시간 배양한다. Electroporation으로 pCIN-mvaA-EGFP 플라스미드를 도입한 세포 시료를 0.5% (v/v) glucose를 첨가한 M17 고체 평판배지에 101-103 희석하여 도말하여 MG1363ΔmvaA(pCIN-mvaA-EGFP) 형질전환체를 얻는다.
iii. GFP 단백질을 이용하여 단백질 발현의 안정성 확인
상기 구축된 MG1363ΔmvaA(pCIN-mvaA-EGFP) 균주를 항생제를 포함하지 않은 0.5% (v/v) glucose를 첨가한 M17 고체 평판배지에 도말하여 형광을 관찰한다.
상기 구축된 재조합 균주의 목표 단백질의 발현을 확실히 하기 위하여 배양을 진행한다. 상세히 설명하자면 균주를 무항생제 조건에서 종균배양하고, 생산배지인 0.5% (v/v) glucose가 첨가된 M17 배지 5ml에 OD600nm 0.1이 되도록 접종하여 30℃에서 배양한다. 이 배양액을 채취하여 무항생제 배지 조건에서 플라스미드에 함유된 유전자의 발현의 안정성을 형광측정을 통하여 확인한다.
| 서열번호 | 프라이머 이름 | 서열번호 | 프라이머 이름 |
| 1 | SN12Didi-F | 36 | RET-R |
| 2 | SN12Didi-R | 37 | RET-F |
| 3 | IS poxB-Ptrc-F | 38 | dAmp(RET)-R |
| 4 | KO poxB -R | 39 | dxr_1-F |
| 5 | IS ldhA-Ptrc-F | 40 | dxr_1-R |
| 6 | KO ldhA-R | 41 | dxs_1-F |
| 7 | IS adhE-Ptrc-F | 42 | dxs_1-R |
| 8 | KO adhE-R | 43 | idi-F |
| 9 | IS atoDA-Ptrc-F | 44 | idi-R |
| 10 | KO atoDA-R | 45 | dxr_3-F |
| 11 | KOpoxBCF-F | 46 | dxr_3-R |
| 12 | KOpoxBCF-R | 47 | dxs_2-F |
| 13 | KOldhACF-F | 48 | dxs_2-R |
| 14 | KOldhACF-R | 49 | dmvaE-F |
| 15 | KOadhECF-F | 50 | dmvaE-R |
| 16 | KOadhECF-R | 51 | dxr_2-F |
| 17 | KOatoDACF-F | 52 | dxr_2-R |
| 18 | KOatoDACF-R | 53 | RET_2-F |
| 19 | KO dxr-F | 54 | RET_2-R |
| 20 | KO dxr-R | 55 | mvaA u/s-Fwd |
| 21 | KOdxrCF-F | 56 | mvaA u/s-Rev |
| 22 | KOdxrCF-R | 57 | mvaA d/s-Fwd |
| 23 | dxrCF-R | 58 | mvaA d/s-Rev |
| 24 | KO dxs-F | 59 | mvaA ex-Fwd |
| 25 | KO dxs-R | 60 | mvaA ex-Rev |
| 26 | KOdxsCF-F | 61 | pORI19-Rev |
| 27 | KOdxsCF-R | 62 | pORI19-Fwd |
| 28 | KmCF-F | 63 | DCO-Fwd |
| 29 | dCmp(NA)-F | 64 | DCO-Rev |
| 30 | dCmp(NA)-R | 65 | SCO u/s-Rev |
| 31 | ddxs(RET)-F | 66 | SCO u/s-Fwd |
| 32 | ddxs(RET)-R | 67 | mvaA-Fwd |
| 33 | P1mvaE-F | 68 | mvaA-Rev |
| 34 | P1mvaE-R | 69 | EGFP-Fwd |
| 35 | dAmp(RET)-F | 70 | EGFP-Rev |
| 유전자명 | 효소 | 유래 | Genbank 허가번호 | 염기서열 | 아미노산서열 |
| 서열번호 | 서열번호 | ||||
| mvaE | 아세틸-CoA 아세틸트란스퍼라제/하이드록시메틸글루타릴 (HMG)-CoA 리덕타제 | 엔테로코커스 패칼리스(Enterococcus faecalis) | AF290092 | 104 | 124 |
| mvaS | HMG-CoA 신타제 | 엔테로코커스 패칼리스(Enterococcus faecalis) | AF290092 | 105 | 125 |
| mvaK1 | 메발로네이트 키나제 | 스트렙토코커스 뉴모니아(Streptococcus pneumoniae) | AF290099 | 106 | 126 |
| mvaK2 | 포스포메발로네이트 키나제 | 스트렙토코커스 뉴모니아 (Streptococcus pneumoniae) | AF290099 | 107 | 127 |
| mvaD | 메발로네이트 디포스페이트 데카르복실라제 | 스트렙토코커스 뉴모니아 (Streptococcus pneumoniae) | AF290099 | 108 | 128 |
| Idi | IPP 이소머라제 | 대장균(Escherichia coli) | U00096 | 109 | 129 |
| ipiHp1 | IPP 이소포머레제 | 헤마토코커스 플루비알리스 (Haematococcuspluvialis) | AF082325 | 110 | 130 |
| crtE | 제라닐제라닐 피로포스페이트 (GGPP) 신타제 | 판토에아 아글루메란스 (pantoea agglomerans) | M87280 | 111 | 131 |
| crtB | 피토엔 신타제 | 판토에아 아글루메란스 (pantoea agglomerans) | M87280 | 112 | 132 |
| crtI | 피토엔 데히드로게나 제 |
판토에아 아글루메란스 (pantoea agglomerans) | M87280 | 113 | 133 |
| crtY | 라이코펜-β-시클라제 | 판토에아 아나나티스 (pantoea ananatis) | D90087 | 114 | 134 |
| SR | β-카로틴모노옥시게나제 | 배양되지 않은 해양 박테리아 (unculturedmarine bacterium) 66A03 | blh의 대장균 코돈최적화 서열 | 115 | 135 |
| YbbO | 산화환원효소 | 야생형 대장균(Escherichia coli MG1655; taxid 511145) |
1786701 | 116 | 136 |
| dxs | 1-데옥시자일룰로즈-5-포스페이트 (DXP) 신타제 |
대장균(Escherichia coli) | AF035440.1 | 117 | 137 |
| dxr | 1-데옥시-D-자일룰로스-50포스페이트 리덕토이소머레제(1-deoxy-D-xylulose 5-phosphate reductoisomerase) | 대장균(Escherichia coli) | AB013300.1 | 118 | 138 |
| ispA | 파네실 파이로포스페이트 신타제 | 대장균(Escherichia coli) | - | 119 | 139 |
| STS | 산탈렌 신타제 | Clausena lansium | - | 120 | 140 |
| MrBBS | α-비사볼롤 신타제 | Matricaria recutita | KM259907.1의 대장균 코돈최적화 서열 | 121 | 141 |
| EGFP | enhanced green fluorescent protein | Synthetic construct | KX130867.1 | 122 | 142 |
| mvaA | Hydroxymethylglutaryl-CoA reductase | Lactococcus lactis subsp. cremoris MG1363 | (WP_011834877.1) | 123 | 143 |
<110> GYEONGSANG NATIONAL UNIVERSITY OFFICE OF ACADEMY AND INDUSTRY COLLABORATION
<120> MARKER COMPOSITION FOR TRANSFORMED MICROORGANISM, TRANSFORMED
MICROORGANISM AND METHOD FOR TRANSFORMATION
<130> 17P04058
<150> KR 10-2017-0058829
<151> 2017-05-11
<160> 143
<170> KopatentIn 2.0
<210> 1
<211> 46
<212> DNA
<213> Artificial Sequence
<220>
<223> SN12Didi-F
<400> 1
gcgaattcag gaggtaataa tatgacaaaa aaagttggtg tcggtc 46
<210> 2
<211> 39
<212> DNA
<213> Artificial Sequence
<220>
<223> SN12Didi-R
<400> 2
cgcctgcagg ttatttaagc tgggtaaatg cagataatc 39
<210> 3
<211> 74
<212> DNA
<213> Artificial Sequence
<220>
<223> IS poxB-Ptrc-F
<400> 3
gatgaactaa acttgttacc gttatcacat tcaggagatg gagaaccatg gtttgacagc 60
ttatcatcga ctgc 74
<210> 4
<211> 70
<212> DNA
<213> Artificial Sequence
<220>
<223> KO poxB -R
<400> 4
ccttattatg acgggaaatg ccaccctttt taccttagcc agtttgtttt tgtaggctgg 60
agctgcttcg 70
<210> 5
<211> 74
<212> DNA
<213> Artificial Sequence
<220>
<223> IS ldhA-Ptrc-F
<400> 5
atttttagta gcttaaatgt gattcaacat cactggagaa agtcttatga gtttgacagc 60
ttatcatcga ctgc 74
<210> 6
<211> 70
<212> DNA
<213> Artificial Sequence
<220>
<223> KO ldhA-R
<400> 6
ctcccctgga atgcagggga gcggcaagat taaaccagtt cgttcgggca tgtaggctgg 60
agctgcttcg 70
<210> 7
<211> 74
<212> DNA
<213> Artificial Sequence
<220>
<223> IS adhE-Ptrc-F
<400> 7
cgagcagatg atttactaaa aaagtttaac attatcagga gagcattatg gtttgacagc 60
ttatcatcga ctgc 74
<210> 8
<211> 70
<212> DNA
<213> Artificial Sequence
<220>
<223> KO adhE-R
<400> 8
ccgtttatgt tgccagacag cgctactgat taagcggatt ttttcgcttt tgtaggctgg 60
agctgcttcg 70
<210> 9
<211> 74
<212> DNA
<213> Artificial Sequence
<220>
<223> IS atoDA-Ptrc-F
<400> 9
ctattgcctg actgtaccca caacggtgta tgcaagaggg ataaaaaatg gtttgacagc 60
ttatcatcga ctgc 74
<210> 10
<211> 70
<212> DNA
<213> Artificial Sequence
<220>
<223> KO atoDA-R
<400> 10
acgcgtcata aaacgcgata tgcgaccaat cataaatcac cccgttgcgt tgtaggctgg 60
agctgcttcg 70
<210> 11
<211> 22
<212> DNA
<213> Artificial Sequence
<220>
<223> KOpoxBCF-F
<400> 11
ttacgtactg gcctgctcct gc 22
<210> 12
<211> 22
<212> DNA
<213> Artificial Sequence
<220>
<223> KOpoxBCF-R
<400> 12
gtcgggtaac ggtatcactg cg 22
<210> 13
<211> 20
<212> DNA
<213> Artificial Sequence
<220>
<223> KOldhACF-F
<400> 13
tcatcagcag cgtcaacggc 20
<210> 14
<211> 20
<212> DNA
<213> Artificial Sequence
<220>
<223> KOldhACF-R
<400> 14
cgctggtcac gggcttaccg 20
<210> 15
<211> 20
<212> DNA
<213> Artificial Sequence
<220>
<223> KOadhECF-F
<400> 15
ccgcactgac tatactctcg 20
<210> 16
<211> 20
<212> DNA
<213> Artificial Sequence
<220>
<223> KOadhECF-R
<400> 16
tgatcggcat tgcccagaag 20
<210> 17
<211> 22
<212> DNA
<213> Artificial Sequence
<220>
<223> KOatoDACF-F
<400> 17
ctggcgaggt aaaaacagcc cc 22
<210> 18
<211> 22
<212> DNA
<213> Artificial Sequence
<220>
<223> KOatoDACF-R
<400> 18
aagcgcgatc acgaatgtta gc 22
<210> 19
<211> 65
<212> DNA
<213> Artificial Sequence
<220>
<223> KO dxr-F
<400> 19
ggctggcggc gttttgcttt ttattctgtc tcaactctgg atgtttcatg ggggatccgt 60
cgacc 65
<210> 20
<211> 70
<212> DNA
<213> Artificial Sequence
<220>
<223> KO dxr-R
<400> 20
tagcgcgact ctctgtagcc ggattatcct cagcttgcga gacgcatcac tgtaggctgg 60
agctgcttcg 70
<210> 21
<211> 24
<212> DNA
<213> Artificial Sequence
<220>
<223> KOdxrCF-F
<400> 21
ctgtgtgtga ctgtctggtc tgac 24
<210> 22
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> KOdxrCF-R
<400> 22
catctgtaaa gtaaagttcg gcatagg 27
<210> 23
<211> 20
<212> DNA
<213> Artificial Sequence
<220>
<223> dxrCF-R
<400> 23
ccattttgct caaggtcagc 20
<210> 24
<211> 70
<212> DNA
<213> Artificial Sequence
<220>
<223> KO dxs-F
<400> 24
tttcttaagc atagcaggag tggagtaggg attatgccag ccaggccttg caattcgatg 60
gggatccgtc 70
<210> 25
<211> 70
<212> DNA
<213> Artificial Sequence
<220>
<223> KO dxs-R
<400> 25
gtattaatag gcccctgatg agttttgata ttgccaaata cccgaccctg tgtaggctgg 60
agctgcttcg 70
<210> 26
<211> 17
<212> DNA
<213> Artificial Sequence
<220>
<223> KOdxsCF-F
<400> 26
ggaaacgcga aggtcgg 17
<210> 27
<211> 20
<212> DNA
<213> Artificial Sequence
<220>
<223> KOdxsCF-R
<400> 27
gcggactaca tcatccagcg 20
<210> 28
<211> 20
<212> DNA
<213> Artificial Sequence
<220>
<223> KmCF-F
<400> 28
ccttgctcct gccgagaaag 20
<210> 29
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> dCmp(NA)-F
<400> 29
tttttttaag gcagttattg gtg 23
<210> 30
<211> 20
<212> DNA
<213> Artificial Sequence
<220>
<223> dCmp(NA)-R
<400> 30
agcttcctta gctcctgaaa 20
<210> 31
<211> 20
<212> DNA
<213> Artificial Sequence
<220>
<223> ddxs(RET)-F
<400> 31
gtacccagct tggctgtttt 20
<210> 32
<211> 20
<212> DNA
<213> Artificial Sequence
<220>
<223> ddxs(RET)-R
<400> 32
catggtgaat tcctcctgct 20
<210> 33
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> P1mvaE-F
<400> 33
ttgcggccgc gctgttgaca attaatcatc cg 32
<210> 34
<211> 30
<212> DNA
<213> Artificial Sequence
<220>
<223> P1mvaE-R
<400> 34
ttctcgagcc tgaaacggct acctaatgtg 30
<210> 35
<211> 29
<212> DNA
<213> Artificial Sequence
<220>
<223> dAmp(RET)-F
<400> 35
cccaagcttc gtcagacccc gtagaaaag 29
<210> 36
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> RET-R
<400> 36
cctcgagtta tcaggcgatt ttc 23
<210> 37
<211> 21
<212> DNA
<213> Artificial Sequence
<220>
<223> RET-F
<400> 37
gcctgataac tcgaggaggt a 21
<210> 38
<211> 31
<212> DNA
<213> Artificial Sequence
<220>
<223> dAmp(RET)-R
<400> 38
cccaagcttt gagcggatac atatttgaat g 31
<210> 39
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> dxr_1-F
<400> 39
aaaagatctg tgtggaattg tgagcggata ac 32
<210> 40
<211> 30
<212> DNA
<213> Artificial Sequence
<220>
<223> dxr_1-R
<400> 40
aaaactcgag tagatcagct tgcgagacgc 30
<210> 41
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> dxs_1-F
<400> 41
aaaagatctg tgtggaattg tgagcggata ac 32
<210> 42
<211> 29
<212> DNA
<213> Artificial Sequence
<220>
<223> dxs_1-R
<400> 42
aaaactcgag ttatgccagc caggccttg 29
<210> 43
<211> 46
<212> DNA
<213> Artificial Sequence
<220>
<223> idi-f
<400> 43
acggatcctg aggaggtaac gtatgcaaac ggaacacgtc atttta 46
<210> 44
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> idi-r
<400> 44
tatcgtcgac tctaagatct tatttaagct gggtaaatgc ag 42
<210> 45
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> dxr_3-F
<400> 45
aaagtcgacg tgtggaattg tgagcggata ac 32
<210> 46
<211> 30
<212> DNA
<213> Artificial Sequence
<220>
<223> dxr_3-R
<400> 46
ttaaaagctt tagatcagct tgcgagacgc 30
<210> 47
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> dxs_2-F
<400> 47
aaaactcgag tgtggaattg tgagcggata ac 32
<210> 48
<211> 28
<212> DNA
<213> Artificial Sequence
<220>
<223> dxs_2-R
<400> 48
aaaagatctt tatgccagcc aggccttg 28
<210> 49
<211> 30
<212> DNA
<213> Artificial Sequence
<220>
<223> dmvaE-F
<400> 49
aaaagatctc attaggtagc cgtttcaggc 30
<210> 50
<211> 38
<212> DNA
<213> Artificial Sequence
<220>
<223> dmvaE-R
<400> 50
aaaagatctt tctcgagact ccatggtctg tttcctcg 38
<210> 51
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> dxr_2-F
<400> 51
cccagtactg tgtggaattg tgagcggata ac 32
<210> 52
<211> 29
<212> DNA
<213> Artificial Sequence
<220>
<223> dxr_2-R
<400> 52
cccgctagct agatcagctt gcgagacgc 29
<210> 53
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> RET_2-F
<400> 53
cccgctagca cagaatttgc ctggcgg 27
<210> 54
<211> 31
<212> DNA
<213> Artificial Sequence
<220>
<223> RET_2-R
<400> 54
cccagtactc atgcctgcag ttaacgatga g 31
<210> 55
<211> 22
<212> DNA
<213> Artificial Sequence
<220>
<223> mvaA u/s-Fwd
<400> 55
ccagcttcaa ccattaatga ag 22
<210> 56
<211> 53
<212> DNA
<213> Artificial Sequence
<220>
<223> mvaA u/s-Rev
<400> 56
caggctatta ttttctcaaa ttttttagtt aaaatttttt tctcatattt ccc 53
<210> 57
<211> 51
<212> DNA
<213> Artificial Sequence
<220>
<223> mvaA d/s-Fwd
<400> 57
gggaaatatg agaaaaaaat tttaactaaa aaatttgaga aaataatagc c 51
<210> 58
<211> 19
<212> DNA
<213> Artificial Sequence
<220>
<223> mvaA d/s-Rev
<400> 58
gaatgacgag tggatcacc 19
<210> 59
<211> 24
<212> DNA
<213> Artificial Sequence
<220>
<223> mvaA ex-Fwd
<400> 59
ccattaatga agtttgtggt tcag 24
<210> 60
<211> 21
<212> DNA
<213> Artificial Sequence
<220>
<223> mvaA ex-Rev
<400> 60
cccttacatc ctctgtctca c 21
<210> 61
<211> 21
<212> DNA
<213> Artificial Sequence
<220>
<223> pORI19-Rev
<400> 61
taccgagctc gaattcactg g 21
<210> 62
<211> 20
<212> DNA
<213> Artificial Sequence
<220>
<223> pORI19-Fwd
<400> 62
atcctctaga gtcgacctgc 20
<210> 63
<211> 21
<212> DNA
<213> Artificial Sequence
<220>
<223> DCO-Fwd
<400> 63
cgccttaaga acaccagtcg g 21
<210> 64
<211> 19
<212> DNA
<213> Artificial Sequence
<220>
<223> DCO-Rev
<400> 64
gctctccgta ggtcatggc 19
<210> 65
<211> 22
<212> DNA
<213> Artificial Sequence
<220>
<223> SCO u/s-Rev
<400> 65
acaatttcac acaggaaaca gc 22
<210> 66
<211> 21
<212> DNA
<213> Artificial Sequence
<220>
<223> SCO u/s-Fwd
<400> 66
cccccattaa gtgccgagtg c 21
<210> 67
<211> 52
<212> DNA
<213> Artificial Sequence
<220>
<223> mvaA-Fwd
<400> 67
cgcggatcca ggaggtaata aatatgagaa aaaaatttta tcaaatgtcg cc 52
<210> 68
<211> 39
<212> DNA
<213> Artificial Sequence
<220>
<223> mvaA-Rev
<400> 68
gtctagatta ttttctcaaa ttttttagta aattttggg 39
<210> 69
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> EGFP-Fwd
<400> 69
cgcgtcgaca ggaggtaata aatatggtga gcaagggcga gg 42
<210> 70
<211> 31
<212> DNA
<213> Artificial Sequence
<220>
<223> EGFP-Rev
<400> 70
ggcatgctta cttgtacagc tcgtccatgc c 31
<210> 71
<211> 2999
<212> DNA
<213> Artificial Sequence
<220>
<223> pSTV28
<400> 71
cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc 60
gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc 120
cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat 180
ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc 240
accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg 300
ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat 360
gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat 420
gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt 480
gctacgcctg aataagtgat aataagcgga tgaatggcag aaattcgaaa gcaaattcga 540
cccggtcgtc ggttcagggc agggtcgtta aatagccgct tatgtctatt gctggtttac 600
cggtttattg actaccggaa gcagtgtgac cgtgtgcttc tcaaatgcct gaggccagtt 660
tgctcaggct ctccccgtgg aggtaataat tgacgatatg atcatttatt ctgcctccca 720
gagcctgata aaaacggtta gcgcttcgtt aatacagatg taggtgttcc acagggtagc 780
cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgcttg tttcggcgtg 840
ggtatggtgg caggccccgt ggccggggga ctgttgggcg ctgccggcac ctgtcctacg 900
agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac 960
cggaaggagc taccggacag cggtgcggac tgttgtaact cagaataaga aatgaggccg 1020
ctcatggcgt tccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca 1080
gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga 1140
gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt 1200
gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgaat 1260
tcgagctcgg tacccgggga tcctctagag tcgacctgca ggcatgcaag cttggcactg 1320
gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt 1380
gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct 1440
tcccaacagt tgcgcagcct gaatggcgaa tgagcttatc gatgataagc tgtcaaacat 1500
gagaattaca acttatatcg tatggggctg acttcaggtg ctacatttga agagataaat 1560
tgcactgaaa tctagaaata ttttatctga ttaataagat gatcttcttg agatcgtttt 1620
ggtctgcgcg taatctcttg ctctgaaaac gaaaaaaccg ccttgcaggg cggtttttcg 1680
aaggttctct gagctaccaa ctctttgaac cgaggtaact ggcttggagg agcgcagtca 1740
ccaaaacttg tcctttcagt ttagccttaa ccggcgcatg acttcaagac taactcctct 1800
aaatcaatta ccagtggctg ctgccagtgg tgcttttgca tgtctttccg ggttggactc 1860
aagacgatag ttaccggata aggcgcagcg gtcggactga acggggggtt cgtgcataca 1920
gtccagcttg gagcgaactg cctacccgga actgagtgtc aggcgtggaa tgagacaaac 1980
gcggccataa cagcggaatg acaccggtaa accgaaaggc aggaacagga gagcgcacga 2040
gggagccgcc aggggaaacg cctggtatct ttatagtcct gtcgggtttc gccaccactg 2100
atttgagcgt cagatttcgt gatgcttgtc aggggggcgg agcctatgga aaaacggctt 2160
tgccgcggcc ctctcacttc cctgttaagt atcttcctgg catcttccag gaaatctccg 2220
ccccgttcgt aagccatttc cgctcgccgc agtcgaacga ccgagcgtag cgagtcagtg 2280
agcgaggaag cggaatatat cctgtatcac atattctgct gacgcaccgg tgcagccttt 2340
tttctcctgc cacatgaagc acttcactga caccctcatc agtgccaaca tagtaagcca 2400
gtatacactc cgctagcgct gatgtccggc ggtgcttttg ccgttacgca ccaccccgtc 2460
agtagctgaa caggagggac agctgataga aacagaagcc actggagcac ctcaaaaaca 2520
ccatcataca ctaaatcagt aagttggcag catcacccga cgcactttgc gccgaataaa 2580
tacctgtgac ggaagatcac ttcgcagaat aaataaatcc tggtgtccct gttgataccg 2640
ggaagccctg ggccaacttt tggcgaaaat gagacgttga tcggcacgta agaggttcca 2700
actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt 2760
caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat 2820
cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata 2880
accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca 2940
agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattt 2999
<210> 72
<211> 4176
<212> DNA
<213> Artificial Sequence
<220>
<223> pTrc99A
<400> 72
gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc 60
ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc 120
gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc 180
tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga 240
taacaatttc acacaggaaa cagaccatgg aattcgagct cggtacccgg ggatcctcta 300
gagtcgacct gcaggcatgc aagcttggct gttttggcgg atgagagaag attttcagcc 360
tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca 420
gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg 480
atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga 540
aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 600
ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg 660
tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg 720
acggatggcc tttttgcgtt tctacaaact ctttttgttt atttttctaa atacattcaa 780
atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 840
agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 900
ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 960
gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 1020
gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 1080
tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 1140
acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 1200
aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 1260
cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 1320
gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 1380
cgatgcctac agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 1440
tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 1500
tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 1560
ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 1620
tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 1680
gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 1740
ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 1800
tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 1860
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 1920
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 1980
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 2040
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 2100
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 2160
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 2220
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 2280
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 2340
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 2400
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 2460
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 2520
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 2580
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 2640
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 2700
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 2760
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 2820
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 2880
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agcagatcaa 2940
ttcgcgcgcg aaggcgaagc ggcatgcatt tacgttgaca ccatcgaatg gtgcaaaacc 3000
tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa 3060
ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc 3120
gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg 3180
gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg 3240
ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg 3300
attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc 3360
ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg 3420
atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat 3480
gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc 3540
catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc 3600
gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat 3660
aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc 3720
atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg 3780
ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg 3840
cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat 3900
atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac 3960
cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca 4020
ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg 4080
gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg 4140
caacgcaatt aatgtgagtt agcgcgaatt gatctg 4176
<210> 73
<211> 4620
<212> DNA
<213> Artificial Sequence
<220>
<223> pTFKC(DPB)
<400> 73
atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 60
gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 120
atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 180
tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 240
cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 300
ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 360
actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta 420
gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 480
ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 540
gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 600
acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 660
tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 720
gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 780
cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 840
cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 900
ccttttgctc acatgtgttt gacagcttat catcgactgc acggtgcacc aatgcttctg 960
gcgtcaggca gccatcggaa gctgtggtat ggctgtgcag gtcgtaaatc actgcataat 1020
tcgtgtcgct caaggcgcac tcccgttctg gataatgttt tttgcgccga catcataacg 1080
gttctggcaa atattctgaa atgagctgtt gacaattaat catccggctc gtataatgtg 1140
tggtcacaca ggaaacagac catggaattc gagctcggta cccggggatc ctctagagtc 1200
gacctgcagg catgcaagct tggctgtttt ggcggatgag agaagatttt cagcctgata 1260
cagattaaat cagaacgcag aagcggtctg ataaaacaga atttgcctgg cggcagtagc 1320
gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt 1380
agtgtggggt ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc 1440
tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag 1500
taggacaaat ccgccgggag cggatttgaa cgttgcgaag caacggcccg gagggtggcg 1560
ggcaggacgc ccgccataaa ctgccaggca tcaaattaag cagaaggcca tcctgacgga 1620
tggccttttt gcgtttctac aaactctttt tgtttatttt tctaaataca ttcaaatatg 1680
tatccgctca tgagacaata acccgataat tcgatgggga tccgtcgacc tgcagttcga 1740
agttcctatt ctctagaaag tataggaact tcagagcgct tttgaagctc acgctgccgc 1800
aagcactcag ggcgcaaggg ctgctaaagg aagcggaaca cgtagaaagc cagtccgcag 1860
aaacggtgct gaccccggat gaatgtcagc tactgggcta tctggacaag ggaaaacgca 1920
agcgcaaaga gaaagcaggt agcttgcagt gggcttacat ggcgatagct agactgggcg 1980
gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg taaggttggg 2040
aagccctgca aagtaaactg gatggctttc ttgccgccaa ggatctgatg gcgcagggga 2100
tcaagatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg 2160
cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag 2220
acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt 2280
tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta 2340
tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg 2400
ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt 2460
gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat 2520
ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg 2580
atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca 2640
gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc 2700
catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc 2760
gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat 2820
attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc 2880
gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctaataaggg 2940
gatcttgaag ttcctattcc gaagttccta ttctctagaa agtataggaa cttcgaagca 3000
gctccagcct acaaggaggt aataaatatg atcaagctta tcgataccgt cgacctcgag 3060
ggggggcccg gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt 3120
cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc 3180
acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca 3240
acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc 3300
gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc 3360
tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa 3420
tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact 3480
tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt 3540
gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa 3600
ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt 3660
aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac 3720
aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 3780
atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 3840
tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 3900
gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 3960
gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 4020
gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 4080
ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 4140
tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 4200
acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 4260
cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 4320
catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 4380
cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 4440
ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 4500
ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 4560
ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 4620
4620
<210> 74
<211> 4335
<212> DNA
<213> Artificial Sequence
<220>
<223> pTFCC(DPB)
<400> 74
gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 60
caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 120
ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 180
gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 240
tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 300
ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 360
tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 420
atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 480
gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 540
caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 600
ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 660
ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 720
ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 780
ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 840
gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 900
ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 960
taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 1020
agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 1080
atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 1140
aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 1200
caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 1260
ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 1320
cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 1380
tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 1440
gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 1500
ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 1560
gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 1620
caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 1680
ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 1740
tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 1800
ctcacatgtg tttgacagct tatcatcgac tgcacggtgc accaatgctt ctggcgtcag 1860
gcagccatcg gaagctgtgg tatggctgtg caggtcgtaa atcactgcat aattcgtgtc 1920
gctcaaggcg cactcccgtt ctggataatg ttttttgcgc cgacatcata acggttctgg 1980
caaatattct gaaatgagct gttgacaatt aatcatccgg ctcgtataat gtgtggtcac 2040
acaggaaaca gaccatggaa ttcgagctcg gtacccgggg atcctctaga gtcgacctgc 2100
aggcatgcaa gcttggctgt tttggcggat gagagaagat tttcagcctg atacagatta 2160
aatcagaacg cagaagcggt ctgataaaac agaatttgcc tggcggcagt agcgcggtgg 2220
tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg 2280
ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg 2340
aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca 2400
aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg gcgggcagga 2460
cgcccgccat aaactgccag gcatcaaatt aagcagaagg ccatcctgac ggatggcctt 2520
tttgcgtttc tacaaactct ttttgtttat ttttctaaat acattcaaat atgtatccgc 2580
tcatgagaca ataacccgat aattcgatca tatgaatatc ctccttagtt cctattccga 2640
agttcctatt ctctagaaag tataggaact tcggcgcgcc tacctgtgac ggaagatcac 2700
ttcgcagaat aaataaatcc tggtgtccct gttgataccg ggaagccctg ggccaacttt 2760
tggcgaaaat gagacgttga tcggcacgta agaggttcca actttcacca taatgaaata 2820
agatcactac cgggcgtatt ttttgagttg tcgagatttt caggagctaa ggaagctaaa 2880
atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa 2940
cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat 3000
attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt 3060
cacattcttg cccgcctgat gaatgctcat ccggaattac gtatggcaat gaaagacggt 3120
gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa 3180
acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat 3240
tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag 3300
aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg 3360
gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc 3420
gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat 3480
gtcggcagat gcttaatgaa tacaacagta ctgcgatgag tggcagggcg gggcgtaagg 3540
cgcgccattt aaatgaagtt cctattccga agttcctatt ctctagaaag tataggaact 3600
tcgaagcagc tccagcctac aaggaggtaa taaatatgat caagcttatc gataccgtcg 3660
acctcgaggg ggggcccggt acccaattcg ccctatagtg agtcgtatta cgcgcgctca 3720
ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc 3780
cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc 3840
ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta 3900
agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 3960
cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 4020
gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 4080
aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 4140
cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 4200
acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc 4260
tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta 4320
acgcttacaa tttag 4335
<210> 75
<211> 9497
<212> DNA
<213> Artificial Sequence
<220>
<223> pCP20
<400> 75
gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt 60
gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt 120
ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga 180
tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga 240
aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt 300
ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc 360
ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat 420
ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt 480
gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg 540
acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgcctgtag tgccatttac 600
ccccattcac tgccagagcc gtgagcgcag cgaactgaat gtcacgaaaa agacagcgac 660
tcaggtgcct gatggtcgga gacaaaagga atattcagcg atttgcccga gcttgcgagg 720
gtgctactta agcctttagg gttttaaggt ctgttttgta gaggagcaaa cagcgtttgc 780
gacatccttt tgtaatactg cggaactgac taaagtagtg agttatacac agggctggga 840
tctattcttt ttatcttttt ttattctttc tttattctat aaattataac cacttgaata 900
taaacaaaaa aaacacacaa aggtctagcg gaatttacag agggtctagc agaatttaca 960
agttttccag caaaggtcta gcagaattta cagataccca caactcaaag gaaaaggact 1020
agtaattatc attgactagc ccatctcaat tggtatagtg attaaaatca cctagaccaa 1080
ttgagatgta tgtctgaatt agttgttttc aaagcaaatg aactagcgat tagtcgctat 1140
gacttaacgg agcatgaaac caagctaatt ttatgctgtg tggcactact caaccccacg 1200
attgaaaacc ctacaaggaa agaacggacg gtatcgttca cttataacca atacgttcag 1260
atgatgaaca tcagtaggga aaatgcttat ggtgtattag ctaaagcaac cagagagctg 1320
atgacgagaa ctgtggaaat caggaatcct ttggttaaag gctttgagat tttccagtgg 1380
acaaactatg ccaagttctc aagcgaaaaa ttagaattag tttttagtga agagatattg 1440
ccttatcttt tccagttaaa aaaattcata aaatataatc tggaacatgt taagtctttt 1500
gaaaacaaat actctatgag gatttatgag tggttattaa aagaactaac acaaaagaaa 1560
actcacaagg caaatataga gattagcctt gatgaattta agttcatgtt aatgcttgaa 1620
aataactacc atgagtttaa aaggcttaac caatgggttt tgaaaccaat aagtaaagat 1680
ttaaacactt acagcaatat gaaattggtg gttgataagc gaggccgccc gactgatacg 1740
ttgattttcc aagttgaact agatagacaa atggatctcg taaccgaact tgagaacaac 1800
cagataaaaa tgaatggtga caaaatacca acaaccatta catcagattc ctacctacgt 1860
aacggactaa gaaaaacact acacgatgct ttaactgcaa aaattcagct caccagtttt 1920
gaggcaaaat ttttgagtga catgcaaagt aagcatgatc tcaatggttc gttctcatgg 1980
ctcacgcaaa aacaacgaac cacactagag aacatactgg ctaaatacgg aaggatctga 2040
ggttcttatg gctcttgtat ctatcagtga agcatcaaga ctaacaaaca aaagtagaac 2100
aactgttcac cgttagatat caaagggaaa actgtccata tgcacagatg aaaacggtgt 2160
aaaaaagata gatacatcag agcttttacg agtttttggt gcatttaaag ctgttcacca 2220
tgaacagatc gacaatgtaa cagatgaaca gcatgtaaca cctaatagaa caggtgaaac 2280
cagtaaaaca aagcaactag aacatgaaat tgaacacctg agacaacttg ttacagctca 2340
acagtcacac atagacagcc tgaaacaggc gatgctgctt atcgaatcaa agctgccgac 2400
aacacgggag ccagtgacgc ctcccgtggg gaaaaaatca tggcaattct ggaagaaaat 2460
agcgcctgtt tcgtttcagg caggttatca gggagtgtca gcgtcctgcg gttctccggg 2520
gcgttcgggt catgcagccc gtaatggtga tttaacagcg tctgccaagc atcaattcta 2580
ggcctgtctg cgcggtcgta gtacggctgg aggcgttttc cggtctgtag ctccatgttc 2640
ggaatgacaa aattcagctc aagccgtccc ttgtcctggt gctccaccca caggatgctg 2700
tactgatttt tttcgagacc gggcatcagt acacgctcaa agctcgccat cactttttca 2760
cgtcctcccg gcggcagctc cttctccgcg aacgacagaa caccggacgt gtatttcttc 2820
gcaaatggcg tggcatcgat gagttcccgg acttcttccg gattaccctg aagcaccgtt 2880
gcgccttcgc ggttacgctc cctccccagc aggtaatcaa ccggaccact gccaccacct 2940
tttcccctgg catgaaattt aactatcatc ccgcgccccc tgttccctga cagccagacg 3000
cagccggcgc agctcatccc cgatggccat cagtgcggcc accacctgaa cccggtcacc 3060
ggaagaccac tgcccgctgt tcaccttacg ggctgtctga ttcaggttat ttccgatggc 3120
ggccagctga cgcagtaacg gcggtgccag tgtcggcagt tttccggaac gggcaaccgg 3180
ctcccccagg cagacccgcc gcatccatac cgccagttgt ttaccctcac agcgttcaag 3240
taaccgggca tgttcatcat cagtaacccg tattgtgagc atcctctcgc gtttcatcgg 3300
tatcattacc ccatgaacag aaatccccct tacacggagg catcagtgac taaacggggt 3360
ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 3420
ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 3480
atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 3540
tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 3600
gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 3660
ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 3720
caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 3780
cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct 3840
cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 3900
cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 3960
agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 4020
tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 4080
agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac 4140
atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 4200
ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 4260
cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 4320
caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 4380
attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 4440
agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct 4500
aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc 4560
gtcttcaaga attttataaa ccgtggagcg ggcaatactg agctgatgag caatttccgt 4620
tgcaccagtg cccttctgat gaagcgtcag cacgacgttc ctgtccacgg tacgcctgcg 4680
gccaaatttg attcctttca gctttgcttc ctgtcggccc tcattcgtgc gctctaggat 4740
cctctacgcc ggacgcatcg tggccggcat caccggcgct gaggtctgcc tcgtgaagaa 4800
ggtgttgctg actcatacca ggcctgaatc gccccatcat ccagccagaa agtgagggag 4860
ccacggttga tgagagcttt gttgtaggtg gaccagttgg tgattttgaa cttttgcttt 4920
gccacggaac ggtctgcgtt gtcgggaaga tgcgtgatct gatccttcaa ctcagcaaaa 4980
gttcgattta ttcaacaaag ccgccgtccc gtcaagtcag cgtaatgctc tgccagtgtt 5040
acaaccaatt aaccaattct gattagaaaa actcatcgag catcaaatga aactgcaatt 5100
tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag 5160
aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga 5220
ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg 5280
agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagcttc tagaggatcc 5340
ccctagagaa taggaacttc ggaataggaa cttcaaagcg tttccgaaaa cgagcgcttc 5400
cgaaaatgca acgcgagctg cgcacataca gctcactgtt cacgtcgcac ctatatctgc 5460
gtgttgcctg tatatatata tacatgagaa gaacggcata gtgcgtgttt atgcttaaat 5520
gcgtacttat atgcgtctat ttatgtagga tgaaaggtag tctagtacct cctgtgatat 5580
tatcccattc catgcggggt atcgtatgct tccttcagca ctacccttta gctgttctat 5640
atgctgccac tcctcaattg gattagtctc atccttcaat gctatcattt cctttgatat 5700
tggatcatat gcatagtacc gagaaactag tgcgaagtag tgatcaggta ttgctgttat 5760
ctgatgagta tacgttgtcc tggccacggc agaagcacgc ttatcgctcc aatttcccac 5820
aacattagtc aactccgtta ggcccttcat tgaaagaaat gaggtcatca aatgtcttcc 5880
aatgtgagat tttgggccat tttttatagc aaagattgaa taaggcgcat ttttcttcaa 5940
agctttattg tacgatctga ctaagttatc ttttaataat tggtattcct gtttattgct 6000
tgaagaattg ccggtcctat ttactcgttt taggactggt tcagaattcc tcaaaaattc 6060
atccaaatat acaagtggat cgatcctacc ccttgcgcta aagaagtata tgtgcctact 6120
aacgcttgtc tttgtctctg tcactaaaca ctggattatt actcccagat acttattttg 6180
gactaattta aatgatttcg gatcaacgtt cttaatatcg ctgaatcttc cacaattgat 6240
gaaagtagct aggaagagga attggtataa agtttttgtt tttgtaaatc tcgaagtata 6300
ctcaaacgaa tttagtattt tctcagtgat ctcccagatg ctttcaccct cacttagaag 6360
tgctttaagc atttttttac tgtggctatt tcccttatct gcttcttccg atgattcgaa 6420
ctgtaattgc aaactactta caatatcagt gatatcagat tgatgttttt gtccatagta 6480
aggaataatt gtaaattccc aagcaggaat caatttcttt aatgaggctt ccagaattgt 6540
tgctttttgc gtcttgtatt taaactggag tgatttattg acaatatcga aactcagcga 6600
attgcttatg atagtattat agctcatgaa tgtggctctc ttgattgctg ttccgttatg 6660
tgtaatcatc caacataaat aggttagttc agcagcacat aatgctattt tctcacctga 6720
aggtctttca aacctttcca caaactgacg aacaagcacc ttaggtggtg ttttacataa 6780
tataccaaat tgtggcatac aacctcctta gtacatgcaa ccattatcac cgccagaggt 6840
aaaatagtca acacgcacgg tgttagatat ttatcccttg cggtgataga tttaacgtat 6900
gagcacaaaa aagaaaccat taacacaaga gcagcttgag gacgcacgtc gccttaaagc 6960
aatttatgaa aaaaagaaaa atgaacttgg cttatcccag gaatctgtcg cagacaagat 7020
ggggatgggg cagtcaggcg ttggtgcttt atttaatggc atcaatgcat taaatgctta 7080
taacgccgca ttgcttacaa aaattctcaa agttagcgtt gaagaattta gcccttcaat 7140
cgccagagaa atctacgaga tgtatgaagc ggttagtatg cagccgtcac ttagaagtga 7200
gtatgagtac cctgtttttt ctcatgttca ggcagggatg ttctcaccta agcttagaac 7260
ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc aaaaaagcca gtgattctgc 7320
attctggctt gaggttgaag gtaattccat gaccgcacca acaggctcca agccaagctt 7380
tcctgacgga atgttaattc tcgttgaccc tgagcaggct gttgagccag gtgatttctg 7440
catagccaga cttgggggtg atgagtttac cttcaagaaa ctgatcaggg atagcggtca 7500
ggtgttttta caaccactaa acccacagta cccaatgatc ccatgcaatg agagttgttc 7560
cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag acgtttggct gatcggcaag 7620
gtgttctggt cggcgcatag ctgataacaa ttgagcaaga atcttcatcg aattagggga 7680
attttcactc ccctcagaac ataacatagt aaatggattg aattatgaag aatggttttt 7740
atgcgactta ccgcagcaaa aataaaggga aagataagcg ctcaataaac ctgtctgttt 7800
tccttaattc tctgctggct gataatcatc acctgcaggt tggctccaat tatttgtata 7860
ttcataaaat cgagcttatg catttctttc cagacttgtt caacaggcca gccattacgc 7920
tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg 7980
agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg 8040
cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat 8100
acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta 8160
cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc 8220
atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc 8280
gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga 8340
gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg cctcgagcaa 8400
gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac 8460
agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga 8520
gacacaacgt ggctttgttg aataaatcga acttttgctg agttgaagga tcagatcacg 8580
catcttcccg acaacgcaga ccgttccgtg gcaaagcaaa agttcaaaat caccaactgg 8640
tccacctaca acaaagctct catcaaccgt ggctccctca ctttctggct ggatgatggg 8700
gcgattcagg cctggtatga gtcagcaaca ccttcttcac gaggcagacc tcagcgctaa 8760
ccgtttttat caggctctgg gaggcagaat aaatgatcat atcgtcaatt attacctcca 8820
cggggagagc ctgagcaaac tggcctcagg catttgagaa gcacacggtc acactgcttc 8880
cggtagtcaa taaaccggta aaccagcaat agacataagc ggctatttaa cgaccctgcc 8940
ctgaaccgac gaccgggtcg aatttgcttt cgaatttctg ccattcatcc gcttattatc 9000
acttattcag gcgtagcacc aggcgtttaa gggcaccaat aactgcctta aaaaaattac 9060
gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg 9120
aagccatcac agacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct 9180
tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg 9240
tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca 9300
ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat 9360
atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca 9420
gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg 9480
tctttcattg ccatacg 9497
<210> 76
<211> 6329
<212> DNA
<213> Artificial Sequence
<220>
<223> pKD46
<400> 76
catcgattta ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac 60
ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat 120
cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca 180
gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct 240
ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga 300
tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat 360
tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct 420
caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga 480
tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg 540
tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt 600
aaacccactg gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc 660
ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca 720
ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt 780
cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg 840
cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac 900
tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg 960
tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt 1020
aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca 1080
gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat 1140
ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat 1200
acccgttttt ttgggaattc gagctctaag gaggttataa aaaatggata ttaatactga 1260
aactgagatc aagcaaaagc attcactaac cccctttcct gttttcctaa tcagcccggc 1320
atttcgcggg cgatattttc acagctattt caggagttca gccatgaacg cttattacat 1380
tcaggatcgt cttgaggctc agagctgggc gcgtcactac cagcagctcg cccgtgaaga 1440
gaaagaggca gaactggcag acgacatgga aaaaggcctg ccccagcacc tgtttgaatc 1500
gctatgcatc gatcatttgc aacgccacgg ggccagcaaa aaatccatta cccgtgcgtt 1560
tgatgacgat gttgagtttc aggagcgcat ggcagaacac atccggtaca tggttgaaac 1620
cattgctcac caccaggttg atattgattc agaggtataa aacgaatgag tactgcactc 1680
gcaacgctgg ctgggaagct ggctgaacgt gtcggcatgg attctgtcga cccacaggaa 1740
ctgatcacca ctcttcgcca gacggcattt aaaggtgatg ccagcgatgc gcagttcatc 1800
gcattactga tcgttgccaa ccagtacggc cttaatccgt ggacgaaaga aatttacgcc 1860
tttcctgata agcagaatgg catcgttccg gtggtgggcg ttgatggctg gtcccgcatc 1920
atcaatgaaa accagcagtt tgatggcatg gactttgagc aggacaatga atcctgtaca 1980
tgccggattt accgcaagga ccgtaatcat ccgatctgcg ttaccgaatg gatggatgaa 2040
tgccgccgcg aaccattcaa aactcgcgaa ggcagagaaa tcacggggcc gtggcagtcg 2100
catcccaaac ggatgttacg tcataaagcc atgattcagt gtgcccgtct ggccttcgga 2160
tttgctggta tctatgacaa ggatgaagcc gagcgcattg tcgaaaatac tgcatacact 2220
gcagaacgtc agccggaacg cgacatcact ccggttaacg atgaaaccat gcaggagatt 2280
aacactctgc tgatcgccct ggataaaaca tgggatgacg acttattgcc gctctgttcc 2340
cagatatttc gccgcgacat tcgtgcatcg tcagaactga cacaggccga agcagtaaaa 2400
gctcttggat tcctgaaaca gaaagccgca gagcagaagg tggcagcatg acaccggaca 2460
ttatcctgca gcgtaccggg atcgatgtga gagctgtcga acagggggat gatgcgtggc 2520
acaaattacg gctcggcgtc atcaccgctt cagaagttca caacgtgata gcaaaacccc 2580
gctccggaaa gaagtggcct gacatgaaaa tgtcctactt ccacaccctg cttgctgagg 2640
tttgcaccgg tgtggctccg gaagttaacg ctaaagcact ggcctgggga aaacagtacg 2700
agaacgacgc cagaaccctg tttgaattca cttccggcgt gaatgttact gaatccccga 2760
tcatctatcg cgacgaaagt atgcgtaccg cctgctctcc cgatggttta tgcagtgacg 2820
gcaacggcct tgaactgaaa tgcccgttta cctcccggga tttcatgaag ttccggctcg 2880
gtggtttcga ggccataaag tcagcttaca tggcccaggt gcagtacagc atgtgggtga 2940
cgcgaaaaaa tgcctggtac tttgccaact atgacccgcg tatgaagcgt gaaggcctgc 3000
attatgtcgt gattgagcgg gatgaaaagt acatggcgag ttttgacgag atcgtgccgg 3060
agttcatcga aaaaatggac gaggcactgg ctgaaattgg ttttgtattt ggggagcaat 3120
ggcgatgacg catcctcacg ataatatccg ggtaggcgca atcactttcg tctactccgt 3180
tacaaagcga ggctgggtat ttcccggcct ttctgttatc cgaaatccac tgaaagcaca 3240
gcggctggct gaggagataa ataataaacg aggggctgta tgcacaaagc atcttctgtt 3300
gagttaagaa cgagtatcga gatggcacat agccttgctc aaattggaat caggtttgtg 3360
ccaataccag tagaaacaga cgaagaatcc atgggtatgg acagttttcc ctttgatatg 3420
taacggtgaa cagttgttct acttttgttt gttagtcttg atgcttcact gatagataca 3480
agagccataa gaacctcaga tccttccgta tttagccagt atgttctcta gtgtggttcg 3540
ttgtttttgc gtgagccatg agaacgaacc attgagatca tacttacttt gcatgtcact 3600
caaaaatttt gcctcaaaac tggtgagctg aatttttgca gttaaagcat cgtgtagtgt 3660
ttttcttagt ccgttacgta ggtaggaatc tgatgtaatg gttgttggta ttttgtcacc 3720
attcattttt atctggttgt tctcaagttc ggttacgaga tccatttgtc tatctagttc 3780
aacttggaaa atcaacgtat cagtcgggcg gcctcgctta tcaaccacca atttcatatt 3840
gctgtaagtg tttaaatctt tacttattgg tttcaaaacc cattggttaa gccttttaaa 3900
ctcatggtag ttattttcaa gcattaacat gaacttaaat tcatcaaggc taatctctat 3960
atttgccttg tgagttttct tttgtgttag ttcttttaat aaccactcat aaatcctcat 4020
agagtatttg ttttcaaaag acttaacatg ttccagatta tattttatga atttttttaa 4080
ctggaaaaga taaggcaata tctcttcact aaaaactaat tctaattttt cgcttgagaa 4140
cttggcatag tttgtccact ggaaaatctc aaagccttta accaaaggat tcctgatttc 4200
cacagttctc gtcatcagct ctctggttgc tttagctaat acaccataag cattttccct 4260
actgatgttc atcatctgag cgtattggtt ataagtgaac gataccgtcc gttctttcct 4320
tgtagggttt tcaatcgtgg ggttgagtag tgccacacag cataaaatta gcttggtttc 4380
atgctccgtt aagtcatagc gactaatcgc tagttcattt gctttgaaaa caactaattc 4440
agacatacat ctcaattggt ctaggtgatt ttaatcacta taccaattga gatgggctag 4500
tcaatgataa ttactagtcc ttttcctttg agttgtgggt atctgtaaat tctgctagac 4560
ctttgctgga aaacttgtaa attctgctag accctctgta aattccgcta gacctttgtg 4620
tgtttttttt gtttatattc aagtggttat aatttataga ataaagaaag aataaaaaaa 4680
gataaaaaga atagatccca gccctgtgta taactcacta ctttagtcag ttccgcagta 4740
ttacaaaagg atgtcgcaaa cgctgtttgc tcctctacaa aacagacctt aaaaccctaa 4800
aggcttaagt agcaccctcg caagctcggt tgcggccgca atcgggcaaa tcgctgaata 4860
ttccttttgt ctccgaccat caggcacctg agtcgctgtc tttttcgtga cattcagttc 4920
gctgcgctca cggctctggc agtgaatggg ggtaaatggc actacaggcg ccttttatgg 4980
attcatgcaa ggaaactacc cataatacaa gaaaagcccg tcacgggctt ctcagggcgt 5040
tttatggcgg gtctgctatg tggtgctatc tgactttttg ctgttcagca gttcctgccc 5100
tctgattttc cagtctgacc acttcggatt atcccgtgac aggtcattca gactggctaa 5160
tgcacccagt aaggcagcgg tatcatcaac ggggtctgac gctcagtgga acgaaaactc 5220
acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 5280
ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 5340
ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 5400
tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 5460
tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 5520
gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 5580
tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 5640
tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 5700
ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 5760
tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 5820
ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 5880
gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 5940
ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 6000
cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 6060
ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 6120
ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 6180
gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 6240
ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 6300
gcgcacattt ccccgaaaag tgccacctg 6329
<210> 77
<211> 6738
<212> DNA
<213> Artificial Sequence
<220>
<223> pT-ispA-STS
<400> 77
gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc 60
ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc 120
gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc 180
tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga 240
taacaatttc acacaggagg taataaatat ggaattcatg gactttccgc agcaactcga 300
agcctgcgtt aagcaggcca accaggcgct gagccgtttt atcgccccac tgccctttca 360
gaacactccc gtggtcgaaa ccatgcagta tggcgcatta ttaggtggta agcgcctgcg 420
acctttcctg gtttatgcca ccggtcatat gttcggcgtt agcacaaaca cgctggacgc 480
acccgctgcc gccgttgagt gtatccacgc ttactcatta attcatgatg atttaccggc 540
aatggatgat gacgatctgc gtcgcggttt gccaacctgc catgtgaagt ttggcgaagc 600
aaacgcgatt ctcgctggcg acgctttaca aacgctggcg ttctcgattt taagcgatgc 660
cgatatgccg gaagtgtcgg accgcgacag aatttcgatg atttctgaac tggcgagcgc 720
cagtggtatt gccggaatgt gcggtggtca ggcattagat ttagacgcgg aaggcaaaca 780
cgtacctctg gacgcgcttg agcgtattca tcgtcataaa accggcgcat tgattcgcgc 840
cgccgttcgc cttggtgcat taagcgccgg agataaagga cgtcgtgctc tgccggtact 900
cgacaagtat gcagagagca tcggccttgc cttccaggtt caggatgaca tcctggatgt 960
ggtgggagat actgcaacgt tgggaaaacg ccagggtgcc gaccagcaac ttggtaaaag 1020
tacctaccct gcacttctgg gtcttgagca agcccggaag aaagcccggg atctgatcga 1080
cgatgcccgt cagtcgctga aacaactggc tgaacagtca ctcgatacct cggcactgga 1140
agcgctagcg gactacatca tccagcgtaa taaataagga tccaaaaagg aggtaataaa 1200
ccatgtcaac tcaacaagtt tcatcagaga acattgttcg taacgctgcg aatttccatc 1260
ctaatatatg gggaaaccat ttcctcacat gtccttctca gacgattgat agttggactc 1320
aacagcacca caaagaactg aaagaagagg tgaggaaaat gatggtgtct gatgcaaata 1380
aacctgccca gagattgcgc ttgattgata ctgtccaaag gctaggtgtg gcttaccact 1440
ttgaaaagga gattgatgat gcattggaga aaataggtca tgaccctttt gatgataaag 1500
atgatctcta cattgtctct ctttgttttc gattgctgag gcagcatgga attaagatat 1560
catgtgatgt gtttgagaag tttaaagatg acgatggaaa attcaaggca tcattgatga 1620
atgatgttca aggcatgcta agtttatatg aggcagcaca cctagccatt cacggagaag 1680
atattttaga tgaagcaatt gttttcacga ccactcacct taagtcaacg gtatctaatt 1740
ctcctgtaaa ctctactttt gctgaacaaa tacgtcattc tctcagagtt cctctccgta 1800
aagctgtacc taggttagag tcgaggtatt tcttggatat ctattcaaga gatgatttgc 1860
acgataaaac tttgctcaat ttcgcaaagt tagactttaa tatactacaa gcaatgcacc 1920
agaaggaagc aagtgagatg accaggtggt ggagagattt tgacttcctt aaaaagctgc 1980
cttatataag agacagagtc gtggagctat atttttggat tctggtggga gtgtcttatc 2040
agcccaaatt cagcactggt agaatttttt tgtccaaaat aatatgcctt gagaccctcg 2100
tagatgatac atttgacgcc tacggtactt ttgacgagct cgcaatcttt actgaagcag 2160
ttacaagatg ggaccttggc cacagagatg cactaccaga atacatgaaa ttcattttca 2220
agacactcat tgatgtctac agtgaagctg agcaagaact ggcaaaggaa gggagatcat 2280
acagcataca ctatgcaata cgatcgttcc aagaactagt tatgaagtac ttctgcgaag 2340
ccaagtggtt aaataaaggt tatgttccga gcctggacga ttataaatca gtttcattaa 2400
gaagtatcgg ttttttaccg atagcggtag cttccttcgt tttcatgggt gatattgcaa 2460
ctaaggaggt ctttgaatgg gaaatgaata accctaagat cataatagcc gcagaaacga 2520
ttttcagatt cctggatgac atagcaggcc ataggtttga gcaaaagaga gaacatagtc 2580
catcagctat tgaatgctac aagaatcaac atggagtgtc tgaggaagag gcagttaaag 2640
cgttgtcgtt agaagttgct aatagttgga aagatataaa tgaggagctg cttctcaacc 2700
caatggctat tcctttacct ctgcttcagg tgattcttga tctctcacgt tcggccgatt 2760
ttatgtacgg taatgctcaa gatcgcttca cgcattcaac gatgatgaaa gaccaagttg 2820
atttggtgct gaaggacccc gttaagcttg acgattaaag atctgtcgac ctgcaggcat 2880
gcaagcttgg ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag 2940
aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac 3000
ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc 3060
cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac 3120
tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg 3180
ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg 3240
ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg 3300
tttctacaaa ctctttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 3360
gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 3420
atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 3480
cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 3540
tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 3600
caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg 3660
ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 3720
cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 3780
taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 3840
agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 3900
cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct acagcaatgg 3960
caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 4020
taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 4080
ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 4140
cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 4200
aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 4260
attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 4320
tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 4380
aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 4440
gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 4500
cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 4560
gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca 4620
agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 4680
ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 4740
cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 4800
acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 4860
gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 4920
ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 4980
agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 5040
cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 5100
tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 5160
gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc 5220
ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta 5280
caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg 5340
ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct 5400
gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag 5460
gttttcaccg tcatcaccga aacgcgcgag gcagcagatc aattcgcgcg cgaaggcgaa 5520
gcggcatgca tttacgttga caccatcgaa tggtgcaaaa cctttcgcgg tatggcatga 5580
tagcgcccgg aagagagtca attcagggtg gtgaatgtga aaccagtaac gttatacgat 5640
gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc 5700
cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt 5760
cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc 5820
tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat 5880
caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa 5940
gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg 6000
gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt 6060
gatgtctctg accagacacc catcaacagt attattttct cccatgaaga cggtacgcga 6120
ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca 6180
ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat 6240
caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa 6300
accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag 6360
atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc 6420
tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc 6480
atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct 6540
cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc 6600
accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 6660
ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag 6720
ttagcgcgaa ttgatctg 6738
<210> 78
<211> 13899
<212> DNA
<213> Artificial Sequence
<220>
<223> pTAS-NA
<400> 78
gatcctacct gacgcttttt atcgcaactc tctactgttt ctccataccc gtttttttgg 60
gctagcagga ggaattcacc atggtaccaa tgacaaaaaa agttggtgtc ggtcaggcac 120
atagtaagat aattttaata ggggaacatg cggtcgttta cggttatcct gccatttccc 180
tgcctctttt ggaggtggag gtgacctgta aggtagttcc tgcagagagt ccttggcgcc 240
tttatgagga ggataccttg tccatggcgg tttatgcctc actggagtat ttgaatatca 300
cagaagcctg cattcgttgt gagattgact cggctatccc tgagaaacgg gggatgggtt 360
cgtcagcggc tatcagcata gcggccattc gtgcagtatt tgactactat caggctgatc 420
tgcctcatga tgtactagaa atcttggtca atcgagctga aatgattgcc catatgaatc 480
ctagtggttt ggatgctaag acctgtctta gtgaccaacc tattcgcttt atcaagaacg 540
taggatttac agaacttgag atggatttat ccgcctattt ggtgattgcc gatacgggtg 600
tttatggtca tactcgtgaa gccatccaag tggttcaaaa taagggcaag gatgccctac 660
cgtttttgca tgccttggga gaattaaccc agcaagcaga agttgcgatt tcacaaaaag 720
atgctgaagg actgggacaa atcctcagtc aagcgcattt acatttaaaa gaaattggag 780
tcagtagccc tgaggcagac tttttggttg aaacgactct tagccatggt gctctgggtg 840
ccaagatgag cggtggtggg ctaggaggtt gtatcatagc cttggtaacc aatttgacac 900
acgcacaaga actagcagaa agattagaag agaaaggagc tgttcagaca tggatagaga 960
gcctgtaaca gtacgttcct acgcaaatat tgctattatc aaatattggg gaaagaaaaa 1020
agaaaaagag atggtgcctg ctactagcag tatttctcta actttggaaa atatgtatac 1080
agagacgacc ttgtcgcctt taccagccaa tgtaacagct gacgaatttt acatcaatgg 1140
tcagctacaa aatgaggtcg agcatgccaa gatgagtaag attattgacc gttatcgtcc 1200
agctggtgag ggctttgtcc gtatcgatac tcaaaacaat atgcctacgg cagcgggcct 1260
gtcctcaagt tctagtggtt tgtccgccct ggtcaaggct tgtaatgctt atttcaagct 1320
tggattggat agaagtcagt tggcacagga agccaaattt gcctcaggct cttcttctcg 1380
gagtttttat ggaccactag gagcctggga taaggatagt ggagaaattt accctgtaga 1440
gacagacttg aaactagcta tgattatgtt ggtgctagag gacaagaaaa aaccaatctc 1500
tagccgtgac gggatgaaac tttgtgtgga aacctcgacg acttttgacg actgggttcg 1560
tcagtctgag aaggactatc aggatatgct gatttatctc aaggaaaatg attttgccaa 1620
gattggagaa ttaacggaga aaaatgctct ggctatgcat gctacgacaa agactgctag 1680
tccagccttt tcttatctga cggatgcctc ttatgaggct atggcctttg ttcgccagct 1740
tcgtgagaaa ggagaggcct gctactttac catggatgct ggtcccaatg ttaaggtctt 1800
ctgtcaggag aaagacttgg agcatttgtc agaaattttc ggtcagcgtt atcgcttgat 1860
tgtgtcaaaa acaaaggatt tgagtcaaga tgattgctgt taaaacttgc ggaaaactct 1920
attgggcagg tgaatatgct attttagagc cagggcagtt agctttgata aaggatattc 1980
ccatctatat gagggctgag attgcttttt ctgacagcta ccgtatctat tcagatatgt 2040
ttgatttcgc agtggactta aggcccaatc ctgactacag cttgattcaa gaaacgattg 2100
ctttgatggg agacttcctc gctgttcgcg gtcagaattt aagacctttt tccctaaaaa 2160
tctgtggcaa aatggaacga gaagggaaaa agtttggtct aggttctagt ggcagcgtcg 2220
ttgtcttggt tgtcaaggct ttactggctc tctataatct ttcggttgat cagaatctct 2280
tgttcaagct gactagcgct gtcttgctca agcgaggaga caatggttcc atgggcgacc 2340
ttgcctgtat tgtggcagag gatttggttc tttaccagtc atttgatcgc cagaaggcgg 2400
ctgcttggtt agaagaagaa aacttggcga cagttctgga gcgtgattgg ggatttttta 2460
tctcacaagt gaaaccaact ttagaatgtg atttcttagt gggatggacc aaggaagtgg 2520
ctgtatcgag tcacatggtc cagcaaatca agcaaaatat caatcaaaat tttttaagtt 2580
cctcaaaaga aacggtggtt tctttggtcg aagccttgga gcaggggaaa gccgaaaaag 2640
ttatcgagca agtagaagta gccagcaagc ttttagaagg cttgagtaca gatatttaca 2700
cgcctttgct tagacagttg aaagaagcca gtcaagattt gcaggccgtt gccaagagta 2760
gtggtgctgg tggtggtgac tgtggcatcg ccctgagttt tgatgcgcaa tcttctcgaa 2820
acactttaaa aaatcgttgg gccgatctgg ggattgagct cttatatcaa gaaaggatag 2880
gacatgacga caaatcgtaa tctagccccg ggaggagaga aattatgcaa acggaacacg 2940
tcattttatt gaatgcacag ggagttccca cgggtacgct ggaaaagtat gccgcacaca 3000
cggcagacac ccgcttacat ctcgcgttct ccagttggct gtttaatgcc aaaggacaat 3060
tattagttac ccgccgcgca ctgagcaaaa aagcatggcc tggcgtgtgg actaactcgg 3120
tttgtgggca cccacaactg ggagaaagca acgaagacgc agtgatccgc cgttgccgtt 3180
atgagcttgg cgtggaaatt acgcctcctg aatctatcta tcctgacttt cgctaccgcg 3240
ccaccgatcc gagtggcatt gtggaaaatg aagtgtgtcc ggtatttgcc gcacgcacca 3300
ctagtgcgtt acagatcaat gatgatgaag tgatggatta tcaatggtgt gatttagcag 3360
atgtattaca cggtattgat gccacgccgt gggcgttcag tccgtggatg gtgatgcagg 3420
cgacaaatcg cgaagccaga aaacgattat ctgcatttac ccagcttaaa taagcatgca 3480
ctagagtcga ggaaacagac catggagttg aaaacagtag ttattattga tgcattacga 3540
acaccaattg gaaaatataa aggcagctta agtcaagtaa gtgccgtaga cttaggaaca 3600
catgttacaa cacaactttt aaaaagacat tccactattt ctgaagaaat tgatcaagta 3660
atctttggaa atgttttaca agctggaaat ggccaaaatc ccgcacgaca aatagcaata 3720
aacagcggtt tatctcatga aattcccgca atgacagtta atgaggtctg cggatcagga 3780
atgaaggccg ttattttggc gaaacaattg attcaattag gagaagcgga agttttaatt 3840
gctggcggga ttgagaatat gtcccaagca cctaaattac aacgatttaa ttacgaaaca 3900
gaaagctatg atgcgccttt ttctagtatg atgtacgatg ggttaacgga tgcctttagt 3960
ggtcaagcaa tgggcttaac tgctgaaaat gtggccgaaa agtatcatgt aactagagaa 4020
gagcaagatc aattttctgt acattcacaa ttaaaagcag ctcaagcaca agcagaaggg 4080
atattcgctg acgaaatagc cccattagaa gtatcaggaa cgcttgtgga gaaagatgaa 4140
gggattcgcc ctaattcgag cgttgagaag ctaggaacgc ttaaaacagt ttttaaagaa 4200
gacggtactg taacagcagg gaatgcatca accattaatg atggggcttc tgctttgatt 4260
attgcttcac aagaatatgc cgaagcacac ggtcttcctt atttagctat tattcgagac 4320
agtgtggaag tcggtattga tccagcctat atgggaattt cgccgattaa agccattcaa 4380
aaactgttag cgcgcaatca acttactacg gaagaaattg atctgtatga aatcaacgaa 4440
gcatttgcag caacttcaat cgtggtccaa agagaactgg ctttaccaga ggaaaaggtc 4500
aacatttatg gtggcggtat ttcattaggt catgcgattg gtgccacagg tgctcgttta 4560
ttaacgagtt taagttatca attaaatcaa aaagaaaaga aatatggagt ggcttcttta 4620
tgtatcggcg gtggcttagg actcgctatg ctactagaga gacctcagca aaaaaaaaac 4680
agccgatttt atcaaatgag tcctgaggaa cgcctggctt ctcttcttaa tgaaggccag 4740
atttctgctg atacaaaaaa agaatttgaa aatacggctt tatcttcgca gattgccaat 4800
catatgattg aaaatcaaat cagtgaaaca gaagtgccga tgggcgttgg cttacattta 4860
acagtggacg aaactgatta tttggtacca atggcgacag aagagccctc agtgattgcg 4920
gctttgagta atggtgcaaa aatagcacaa ggatttaaaa cagtgaatca acaacgttta 4980
atgcgtggac aaatcgtttt ttacgatgtt gcagacgccg agtcattgat tgatgaacta 5040
caagtaagag aaacggaaat ttttcaacaa gcagagttaa gttatccatc tatcgttaaa 5100
cgcggcggcg gcttaagaga tttgcaatat cgtgcttttg atgaatcatt tgtatctgtc 5160
gactttttag tagatgttaa ggatgcaatg ggggcaaata tcgttaacgc tatgttggaa 5220
ggtgtggccg agttgttccg tgaatggttt gcggagcaaa agattttatt cagtatttta 5280
agtaattatg ccacggagtc ggttgttacg atgaaaacgg ctattccagt ttcacgttta 5340
agtaagggga gcaatggccg ggaaattgct gaaaaaattg ttttagcttc acgctatgct 5400
tcattagatc cttatcgggc agtcacgcat aacaaaggga tcatgaatgg cattgaagct 5460
gtcgttttag ctacaggaaa tgatacacgc gctgttagcg cttcttgtca tgcttttgcg 5520
gtgaaggaag gtcgctacca aggtttgact agttggacgc tggatggcga acaactaatt 5580
ggtgaaattt cagttccgct tgcgttagcc acggttggcg gtgccacaaa agtcttacct 5640
aaatctcaag cagctgctga tttgttagca gtgacggatg caaaagaact aagtcgagta 5700
gtagcggctg ttggtttggc acaaaattta gcggcgttac gggccttagt ctctgaagga 5760
attcaaaaag gacacatggc tctacaagca cgttctttag cgatgacggt cggagctact 5820
ggtaaagaag ttgaggcagt cgctcaacaa ttaaaacgtc aaaaaacgat gaaccaagac 5880
cgagccttgg ctattttaaa tgatttaaga aaacaataaa aaaacagttc agcagaaatt 5940
attctgctga actgtttttt ttcacattag gtagccgttt caggccacga gctcaggagt 6000
taaagaaatg acaattggga ttgataaaat tagttttttt gtgccccctt attatattga 6060
tatgacggca ctggctgaag ccagaaatgt agaccctgga aaatttcata ttggtattgg 6120
gcaagaccaa atggcggtga acccaatcag ccaagatatt gtgacatttg cagccaatgc 6180
cgcagaagcg atcttgacca aagaagataa agaggccatt gatatggtga ttgtcgggac 6240
tgagtccagt atcgatgagt caaaagcggc cgcagttgtc ttacatcgtt taatggggat 6300
tcaacctttc gctcgctctt tcgaaatcaa ggaagcttgt tacggagcaa cagcaggctt 6360
acagttagct aagaatcacg tagccttaca tccagataaa aaagtcttgg ttgtagcagc 6420
agatattgca aaatatggat taaattctgg cggtgagcct acacaaggag ctggggcggt 6480
tgcaatgtta gttgctagtg aaccgcgcat cttggcttta aaagaggata atgtgatgct 6540
gacgcaagat atctatgact tttggcgtcc aacaggccat ccgtatccta tggtcgatgg 6600
tcctttgtca aacgaaacct acatccaatc ttttgcccaa gtctgggatg aacataaaaa 6660
aagaaccggt cttgattttg cagattatga tgctttagcg ttccatattc cttacacaaa 6720
aatgggcaaa aaagccttat tagcaaaaat ctccgaccaa actgaagcag aacaggaacg 6780
aattttagcc cgttatgaag aaagcatcat ctatagtcgt cgcgtaggaa acttgtatac 6840
gggttcactt tatctgggac tcatttccct tttagaaaat gcaacgactt taaccgcagg 6900
caatcaaatt gggttattca gttatggttc tggtgctgtc gctgaatttt tcactggtga 6960
attagtagct ggttatcaaa atcatttaca aaaagaaact catttagcac tgctagataa 7020
tcggacagaa ctttctatcg ctgaatatga agccatgttt gcagaaactt tagacacaga 7080
tattgatcaa acgttagaag atgaattaaa atatagtatt tctgctatta ataataccgt 7140
tcgctcttat cgaaactaac tgcagcctcg acctgcaggc atgcaagctt ggctgttttg 7200
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 7260
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 7320
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 7380
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 7440
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 7500
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 7560
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttt 7620
gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 7680
tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta 7740
ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag 7800
taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca 7860
gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta 7920
aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc 7980
gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc 8040
ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca 8100
ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc 8160
acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca 8220
taccaaacga cgagcgtgac accacgatgc ctacagcaat ggcaacaacg ttgcgcaaac 8280
tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg 8340
cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg 8400
ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg 8460
gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac 8520
gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc 8580
aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct 8640
aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 8700
actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 8760
gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 8820
atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 8880
atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 8940
ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 9000
gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 9060
cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 9120
tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 9180
cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 9240
ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 9300
gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 9360
tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg 9420
ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc 9480
gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc 9540
atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg 9600
catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg 9660
acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta 9720
cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc 9780
gaaacgcgcg aggcagcaga tcaattcgcg cgcgaaggcg aagcggcatg catttacgtt 9840
gacaccatcg aatggtgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt 9900
caattcaggg tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt 9960
gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg 10020
cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa 10080
caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac 10140
gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg 10200
gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt 10260
ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt 10320
gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca 10380
cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg 10440
gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg 10500
cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg 10560
gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat 10620
gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg 10680
cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac 10740
gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc 10800
ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag 10860
ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg 10920
caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc 10980
cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagcgcg aattgatctg 11040
gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc 11100
ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc 11160
gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc 11220
tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga 11280
taacaatttc acacaggagg taataaatat ggaattcatg gactttccgc agcaactcga 11340
agcctgcgtt aagcaggcca accaggcgct gagccgtttt atcgccccac tgccctttca 11400
gaacactccc gtggtcgaaa ccatgcagta tggcgcatta ttaggtggta agcgcctgcg 11460
acctttcctg gtttatgcca ccggtcatat gttcggcgtt agcacaaaca cgctggacgc 11520
acccgctgcc gccgttgagt gtatccacgc ttactcatta attcatgatg atttaccggc 11580
aatggatgat gacgatctgc gtcgcggttt gccaacctgc catgtgaagt ttggcgaagc 11640
aaacgcgatt ctcgctggcg acgctttaca aacgctggcg ttctcgattt taagcgatgc 11700
cgatatgccg gaagtgtcgg accgcgacag aatttcgatg atttctgaac tggcgagcgc 11760
cagtggtatt gccggaatgt gcggtggtca ggcattagat ttagacgcgg aaggcaaaca 11820
cgtacctctg gacgcgcttg agcgtattca tcgtcataaa accggcgcat tgattcgcgc 11880
cgccgttcgc cttggtgcat taagcgccgg agataaagga cgtcgtgctc tgccggtact 11940
cgacaagtat gcagagagca tcggccttgc cttccaggtt caggatgaca tcctggatgt 12000
ggtgggagat actgcaacgt tgggaaaacg ccagggtgcc gaccagcaac ttggtaaaag 12060
tacctaccct gcacttctgg gtcttgagca agcccggaag aaagcccggg atctgatcga 12120
cgatgcccgt cagtcgctga aacaactggc tgaacagtca ctcgatacct cggcactgga 12180
agcgctagcg gactacatca tccagcgtaa taaataagga tccaaaaagg aggtaataaa 12240
ccatgtcaac tcaacaagtt tcatcagaga acattgttcg taacgctgcg aatttccatc 12300
ctaatatatg gggaaaccat ttcctcacat gtccttctca gacgattgat agttggactc 12360
aacagcacca caaagaactg aaagaagagg tgaggaaaat gatggtgtct gatgcaaata 12420
aacctgccca gagattgcgc ttgattgata ctgtccaaag gctaggtgtg gcttaccact 12480
ttgaaaagga gattgatgat gcattggaga aaataggtca tgaccctttt gatgataaag 12540
atgatctcta cattgtctct ctttgttttc gattgctgag gcagcatgga attaagatat 12600
catgtgatgt gtttgagaag tttaaagatg acgatggaaa attcaaggca tcattgatga 12660
atgatgttca aggcatgcta agtttatatg aggcagcaca cctagccatt cacggagaag 12720
atattttaga tgaagcaatt gttttcacga ccactcacct taagtcaacg gtatctaatt 12780
ctcctgtaaa ctctactttt gctgaacaaa tacgtcattc tctcagagtt cctctccgta 12840
aagctgtacc taggttagag tcgaggtatt tcttggatat ctattcaaga gatgatttgc 12900
acgataaaac tttgctcaat ttcgcaaagt tagactttaa tatactacaa gcaatgcacc 12960
agaaggaagc aagtgagatg accaggtggt ggagagattt tgacttcctt aaaaagctgc 13020
cttatataag agacagagtc gtggagctat atttttggat tctggtggga gtgtcttatc 13080
agcccaaatt cagcactggt agaatttttt tgtccaaaat aatatgcctt gagaccctcg 13140
tagatgatac atttgacgcc tacggtactt ttgacgagct cgcaatcttt actgaagcag 13200
ttacaagatg ggaccttggc cacagagatg cactaccaga atacatgaaa ttcattttca 13260
agacactcat tgatgtctac agtgaagctg agcaagaact ggcaaaggaa gggagatcat 13320
acagcataca ctatgcaata cgatcgttcc aagaactagt tatgaagtac ttctgcgaag 13380
ccaagtggtt aaataaaggt tatgttccga gcctggacga ttataaatca gtttcattaa 13440
gaagtatcgg ttttttaccg atagcggtag cttccttcgt tttcatgggt gatattgcaa 13500
ctaaggaggt ctttgaatgg gaaatgaata accctaagat cataatagcc gcagaaacga 13560
ttttcagatt cctggatgac atagcaggcc ataggtttga gcaaaagaga gaacatagtc 13620
catcagctat tgaatgctac aagaatcaac atggagtgtc tgaggaagag gcagttaaag 13680
cgttgtcgtt agaagttgct aatagttgga aagatataaa tgaggagctg cttctcaacc 13740
caatggctat tcctttacct ctgcttcagg tgattcttga tctctcacgt tcggccgatt 13800
ttatgtacgg taatgctcaa gatcgcttca cgcattcaac gatgatgaaa gaccaagttg 13860
atttggtgct gaaggacccc gttaagcttg acgattaaa 13899
<210> 79
<211> 10342
<212> DNA
<213> Artificial Sequence
<220>
<223> pSNAK
<400> 79
gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat 60
gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag 120
ctatgaccat gattacgaat tcgagctcgg tacccgggga tcctacctga cgctttttat 180
cgcaactctc tactgtttct ccatacccgt ttttttgggc tagcaggagg aattcaccat 240
ggtaccaatg acaaaaaaag ttggtgtcgg tcaggcacat agtaagataa ttttaatagg 300
ggaacatgcg gtcgtttacg gttatcctgc catttccctg cctcttttgg aggtggaggt 360
gacctgtaag gtagttcctg cagagagtcc ttggcgcctt tatgaggagg ataccttgtc 420
catggcggtt tatgcctcac tggagtattt gaatatcaca gaagcctgca ttcgttgtga 480
gattgactcg gctatccctg agaaacgggg gatgggttcg tcagcggcta tcagcatagc 540
ggccattcgt gcagtatttg actactatca ggctgatctg cctcatgatg tactagaaat 600
cttggtcaat cgagctgaaa tgattgccca tatgaatcct agtggtttgg atgctaagac 660
ctgtcttagt gaccaaccta ttcgctttat caagaacgta ggatttacag aacttgagat 720
ggatttatcc gcctatttgg tgattgccga tacgggtgtt tatggtcata ctcgtgaagc 780
catccaagtg gttcaaaata agggcaagga tgccctaccg tttttgcatg ccttgggaga 840
attaacccag caagcagaag ttgcgatttc acaaaaagat gctgaaggac tgggacaaat 900
cctcagtcaa gcgcatttac atttaaaaga aattggagtc agtagccctg aggcagactt 960
tttggttgaa acgactctta gccatggtgc tctgggtgcc aagatgagcg gtggtgggct 1020
aggaggttgt atcatagcct tggtaaccaa tttgacacac gcacaagaac tagcagaaag 1080
attagaagag aaaggagctg ttcagacatg gatagagagc ctgtaacagt acgttcctac 1140
gcaaatattg ctattatcaa atattgggga aagaaaaaag aaaaagagat ggtgcctgct 1200
actagcagta tttctctaac tttggaaaat atgtatacag agacgacctt gtcgccttta 1260
ccagccaatg taacagctga cgaattttac atcaatggtc agctacaaaa tgaggtcgag 1320
catgccaaga tgagtaagat tattgaccgt tatcgtccag ctggtgaggg ctttgtccgt 1380
atcgatactc aaaacaatat gcctacggca gcgggcctgt cctcaagttc tagtggtttg 1440
tccgccctgg tcaaggcttg taatgcttat ttcaagcttg gattggatag aagtcagttg 1500
gcacaggaag ccaaatttgc ctcaggctct tcttctcgga gtttttatgg accactagga 1560
gcctgggata aggatagtgg agaaatttac cctgtagaga cagacttgaa actagctatg 1620
attatgttgg tgctagagga caagaaaaaa ccaatctcta gccgtgacgg gatgaaactt 1680
tgtgtggaaa cctcgacgac ttttgacgac tgggttcgtc agtctgagaa ggactatcag 1740
gatatgctga tttatctcaa ggaaaatgat tttgccaaga ttggagaatt aacggagaaa 1800
aatgctctgg ctatgcatgc tacgacaaag actgctagtc cagccttttc ttatctgacg 1860
gatgcctctt atgaggctat ggcctttgtt cgccagcttc gtgagaaagg agaggcctgc 1920
tactttacca tggatgctgg tcccaatgtt aaggtcttct gtcaggagaa agacttggag 1980
catttgtcag aaattttcgg tcagcgttat cgcttgattg tgtcaaaaac aaaggatttg 2040
agtcaagatg attgctgtta aaacttgcgg aaaactctat tgggcaggtg aatatgctat 2100
tttagagcca gggcagttag ctttgataaa ggatattccc atctatatga gggctgagat 2160
tgctttttct gacagctacc gtatctattc agatatgttt gatttcgcag tggacttaag 2220
gcccaatcct gactacagct tgattcaaga aacgattgct ttgatgggag acttcctcgc 2280
tgttcgcggt cagaatttaa gacctttttc cctaaaaatc tgtggcaaaa tggaacgaga 2340
agggaaaaag tttggtctag gttctagtgg cagcgtcgtt gtcttggttg tcaaggcttt 2400
actggctctc tataatcttt cggttgatca gaatctcttg ttcaagctga ctagcgctgt 2460
cttgctcaag cgaggagaca atggttccat gggcgacctt gcctgtattg tggcagagga 2520
tttggttctt taccagtcat ttgatcgcca gaaggcggct gcttggttag aagaagaaaa 2580
cttggcgaca gttctggagc gtgattgggg attttttatc tcacaagtga aaccaacttt 2640
agaatgtgat ttcttagtgg gatggaccaa ggaagtggct gtatcgagtc acatggtcca 2700
gcaaatcaag caaaatatca atcaaaattt tttaagttcc tcaaaagaaa cggtggtttc 2760
tttggtcgaa gccttggagc aggggaaagc cgaaaaagtt atcgagcaag tagaagtagc 2820
cagcaagctt ttagaaggct tgagtacaga tatttacacg cctttgctta gacagttgaa 2880
agaagccagt caagatttgc aggccgttgc caagagtagt ggtgctggtg gtggtgactg 2940
tggcatcgcc ctgagttttg atgcgcaatc ttctcgaaac actttaaaaa atcgttgggc 3000
cgatctgggg attgagctct tatatcaaga aaggatagga catgacgaca aatcgtaatc 3060
tagccccggg aggagagaaa ttatgcaaac ggaacacgtc attttattga atgcacaggg 3120
agttcccacg ggtacgctgg aaaagtatgc cgcacacacg gcagacaccc gcttacatct 3180
cgcgttctcc agttggctgt ttaatgccaa aggacaatta ttagttaccc gccgcgcact 3240
gagcaaaaaa gcatggcctg gcgtgtggac taactcggtt tgtgggcacc cacaactggg 3300
agaaagcaac gaagacgcag tgatccgccg ttgccgttat gagcttggcg tggaaattac 3360
gcctcctgaa tctatctatc ctgactttcg ctaccgcgcc accgatccga gtggcattgt 3420
ggaaaatgaa gtgtgtccgg tatttgccgc acgcaccact agtgcgttac agatcaatga 3480
tgatgaagtg atggattatc aatggtgtga tttagcagat gtattacacg gtattgatgc 3540
cacgccgtgg gcgttcagtc cgtggatggt gatgcaggcg acaaatcgcg aagccagaaa 3600
acgattatct gcatttaccc agcttaaata agcatgcact agagtcgagg aaacagacca 3660
tggagttgaa aacagtagtt attattgatg cattacgaac accaattgga aaatataaag 3720
gcagcttaag tcaagtaagt gccgtagact taggaacaca tgttacaaca caacttttaa 3780
aaagacattc cactatttct gaagaaattg atcaagtaat ctttggaaat gttttacaag 3840
ctggaaatgg ccaaaatccc gcacgacaaa tagcaataaa cagcggttta tctcatgaaa 3900
ttcccgcaat gacagttaat gaggtctgcg gatcaggaat gaaggccgtt attttggcga 3960
aacaattgat tcaattagga gaagcggaag ttttaattgc tggcgggatt gagaatatgt 4020
cccaagcacc taaattacaa cgatttaatt acgaaacaga aagctatgat gcgccttttt 4080
ctagtatgat gtacgatggg ttaacggatg cctttagtgg tcaagcaatg ggcttaactg 4140
ctgaaaatgt ggccgaaaag tatcatgtaa ctagagaaga gcaagatcaa ttttctgtac 4200
attcacaatt aaaagcagct caagcacaag cagaagggat attcgctgac gaaatagccc 4260
cattagaagt atcaggaacg cttgtggaga aagatgaagg gattcgccct aattcgagcg 4320
ttgagaagct aggaacgctt aaaacagttt ttaaagaaga cggtactgta acagcaggga 4380
atgcatcaac cattaatgat ggggcttctg ctttgattat tgcttcacaa gaatatgccg 4440
aagcacacgg tcttccttat ttagctatta ttcgagacag tgtggaagtc ggtattgatc 4500
cagcctatat gggaatttcg ccgattaaag ccattcaaaa actgttagcg cgcaatcaac 4560
ttactacgga agaaattgat ctgtatgaaa tcaacgaagc atttgcagca acttcaatcg 4620
tggtccaaag agaactggct ttaccagagg aaaaggtcaa catttatggt ggcggtattt 4680
cattaggtca tgcgattggt gccacaggtg ctcgtttatt aacgagttta agttatcaat 4740
taaatcaaaa agaaaagaaa tatggagtgg cttctttatg tatcggcggt ggcttaggac 4800
tcgctatgct actagagaga cctcagcaaa aaaaaaacag ccgattttat caaatgagtc 4860
ctgaggaacg cctggcttct cttcttaatg aaggccagat ttctgctgat acaaaaaaag 4920
aatttgaaaa tacggcttta tcttcgcaga ttgccaatca tatgattgaa aatcaaatca 4980
gtgaaacaga agtgccgatg ggcgttggct tacatttaac agtggacgaa actgattatt 5040
tggtaccaat ggcgacagaa gagccctcag tgattgcggc tttgagtaat ggtgcaaaaa 5100
tagcacaagg atttaaaaca gtgaatcaac aacgtttaat gcgtggacaa atcgtttttt 5160
acgatgttgc agacgccgag tcattgattg atgaactaca agtaagagaa acggaaattt 5220
ttcaacaagc agagttaagt tatccatcta tcgttaaacg cggcggcggc ttaagagatt 5280
tgcaatatcg tgcttttgat gaatcatttg tatctgtcga ctttttagta gatgttaagg 5340
atgcaatggg ggcaaatatc gttaacgcta tgttggaagg tgtggccgag ttgttccgtg 5400
aatggtttgc ggagcaaaag attttattca gtattttaag taattatgcc acggagtcgg 5460
ttgttacgat gaaaacggct attccagttt cacgtttaag taaggggagc aatggccggg 5520
aaattgctga aaaaattgtt ttagcttcac gctatgcttc attagatcct tatcgggcag 5580
tcacgcataa caaagggatc atgaatggca ttgaagctgt cgttttagct acaggaaatg 5640
atacacgcgc tgttagcgct tcttgtcatg cttttgcggt gaaggaaggt cgctaccaag 5700
gtttgactag ttggacgctg gatggcgaac aactaattgg tgaaatttca gttccgcttg 5760
cgttagccac ggttggcggt gccacaaaag tcttacctaa atctcaagca gctgctgatt 5820
tgttagcagt gacggatgca aaagaactaa gtcgagtagt agcggctgtt ggtttggcac 5880
aaaatttagc ggcgttacgg gccttagtct ctgaaggaat tcaaaaagga cacatggctc 5940
tacaagcacg ttctttagcg atgacggtcg gagctactgg taaagaagtt gaggcagtcg 6000
ctcaacaatt aaaacgtcaa aaaacgatga accaagaccg agccttggct attttaaatg 6060
atttaagaaa acaataaaaa aacagttcag cagaaattat tctgctgaac tgtttttttt 6120
cacattaggt agccgtttca ggccacgagc tcaggagtta aagaaatgac aattgggatt 6180
gataaaatta gtttttttgt gcccccttat tatattgata tgacggcact ggctgaagcc 6240
agaaatgtag accctggaaa atttcatatt ggtattgggc aagaccaaat ggcggtgaac 6300
ccaatcagcc aagatattgt gacatttgca gccaatgccg cagaagcgat cttgaccaaa 6360
gaagataaag aggccattga tatggtgatt gtcgggactg agtccagtat cgatgagtca 6420
aaagcggccg cagttgtctt acatcgttta atggggattc aacctttcgc tcgctctttc 6480
gaaatcaagg aagcttgtta cggagcaaca gcaggcttac agttagctaa gaatcacgta 6540
gccttacatc cagataaaaa agtcttggtt gtagcagcag atattgcaaa atatggatta 6600
aattctggcg gtgagcctac acaaggagct ggggcggttg caatgttagt tgctagtgaa 6660
ccgcgcatct tggctttaaa agaggataat gtgatgctga cgcaagatat ctatgacttt 6720
tggcgtccaa caggccatcc gtatcctatg gtcgatggtc ctttgtcaaa cgaaacctac 6780
atccaatctt ttgcccaagt ctgggatgaa cataaaaaaa gaaccggtct tgattttgca 6840
gattatgatg ctttagcgtt ccatattcct tacacaaaaa tgggcaaaaa agccttatta 6900
gcaaaaatct ccgaccaaac tgaagcagaa caggaacgaa ttttagcccg ttatgaagaa 6960
agcatcatct atagtcgtcg cgtaggaaac ttgtatacgg gttcacttta tctgggactc 7020
atttcccttt tagaaaatgc aacgacttta accgcaggca atcaaattgg gttattcagt 7080
tatggttctg gtgctgtcgc tgaatttttc actggtgaat tagtagctgg ttatcaaaat 7140
catttacaaa aagaaactca tttagcactg ctagataatc ggacagaact ttctatcgct 7200
gaatatgaag ccatgtttgc agaaacttta gacacagata ttgatcaaac gttagaagat 7260
gaattaaaat atagtatttc tgctattaat aataccgttc gctcttatcg aaactaactg 7320
cagcctcgac ctgcaggcat gcaagcttgg cactggccgt cgttttacaa cgtcgtgact 7380
gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct 7440
ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg 7500
gcgaatgagc ttatcgatga taagctgtca aacatgagaa ttacaactta tatcgtatgg 7560
ggctgacttc aggtgctaca tttgaagaga taaattgcac tgaaatctag aaatatttta 7620
tctgattaat aagatgatct tcttgagatc gttttggtct gcgcgtaatc tcttgctctg 7680
aaaacgaaaa aaccgccttg cagggcggtt tttcgaaggt tctctgagct accaactctt 7740
tgaaccgagg taactggctt ggaggagcgc agtcaccaaa acttgtcctt tcagtttagc 7800
cttaaccggc gcatgacttc aagactaact cctctaaatc aattaccagt ggctgctgcc 7860
agtggtgctt ttgcatgtct ttccgggttg gactcaagac gatagttacc ggataaggcg 7920
cagcggtcgg actgaacggg gggttcgtgc atacagtcca gcttggagcg aactgcctac 7980
ccggaactga gtgtcaggcg tggaatgaga caaacgcggc cataacagcg gaatgacacc 8040
ggtaaaccga aaggcaggaa caggagagcg cacgagggag ccgccagggg aaacgcctgg 8100
tatctttata gtcctgtcgg gtttcgccac cactgatttg agcgtcagat ttcgtgatgc 8160
ttgtcagggg ggcggagcct atggaaaaac ggctttgccg cggccctctc acttccctgt 8220
taagtatctt cctggcatct tccaggaaat ctccgccccg ttcgtaagcc atttccgctc 8280
gccgcagtcg aacgaccgag cgtagcgagt cagtgagcga ggaagcggaa tatatcctgt 8340
atcacatatt ctgctgacgc accggtgcag ccttttttct cctgccacat gaagcacttc 8400
actgacaccc tcatcagtgc caacatagta agccagtata cactccgcta gcgctgatgt 8460
ccggcggtgc ttttgccgtt acgcaccacc ccgtcagtag ctgaacagga gggacagctg 8520
atagaaacag aagccactgg agcacctcaa aaacaccatc atacactaaa tcagtaagtt 8580
ggcagcatca cccgacgcac tttgcgccga ataaatacct gtgacggaag atcacttcgc 8640
agaataaata aatcctggtg tccctgttga taccgggaag ccctgggcca acttttggcg 8700
aaaatgagac gttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc 8760
actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgag 8820
ccatattcaa cgggaaacgt cttgctctag gccgcgatta aattccaaca tggatgctga 8880
tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg 8940
attgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc 9000
caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc 9060
gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc 9120
cgggaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga 9180
tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa 9240
cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga 9300
tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat 9360
gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga 9420
taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat 9480
cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc 9540
attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca 9600
gtttcatttg atgctcgatg agtttttcta aagtactgcg atgagtggca gggcggggcg 9660
taattttttt aaggcagtta ttggtgccct taaacgcctg gtgctacgcc tgaataagtg 9720
ataataagcg gatgaatggc agaaattcga aagcaaattc gacccggtcg tcggttcagg 9780
gcagggtcgt taaatagccg cttatgtcta ttgctggttt accggtttat tgactaccgg 9840
aagcagtgtg accgtgtgct tctcaaatgc ctgaggccag tttgctcagg ctctccccgt 9900
ggaggtaata attgacgata tgatcattta ttctgcctcc cagagcctga taaaaacggt 9960
tagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg 10020
cagatccgga acataatggt gcagggcgct tgtttcggcg tgggtatggt ggcaggcccc 10080
gtggccgggg gactgttggg cgctgccggc acctgtccta cgagttgcat gataaagaag 10140
acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctaccggac 10200
agcggtgcgg actgttgtaa ctcagaataa gaaatgaggc cgctcatggc gttccaatac 10260
gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc 10320
ccgactggaa agcgggcagt ga 10342
<210> 80
<211> 9081
<212> DNA
<213> Artificial Sequence
<220>
<223> pSNAK(-E)
<400> 80
aacgctatgt tggaaggtgt ggccgagttg ttccgtgaat ggtttgcgga gcaaaagatt 60
ttattcagta ttttaagtaa ttatgccacg gagtcggttg ttacgatgaa aacggctatt 120
ccagtttcac gtttaagtaa ggggagcaat ggccgggaaa ttgctgaaaa aattgtttta 180
gcttcacgct atgcttcatt agatccttat cgggcagtca cgcataacaa agggatcatg 240
aatggcattg aagctgtcgt tttagctaca ggaaatgata cacgcgctgt tagcgcttct 300
tgtcatgctt ttgcggtgaa ggaaggtcgc taccaaggtt tgactagttg gacgctggat 360
ggcgaacaac taattggtga aatttcagtt ccgcttgcgt tagccacggt tggcggtgcc 420
acaaaagtct tacctaaatc tcaagcagct gctgatttgt tagcagtgac ggatgcaaaa 480
gaactaagtc gagtagtagc ggctgttggt ttggcacaaa atttagcggc gttacgggcc 540
ttagtctctg aaggaattca aaaaggacac atggctctac aagcacgttc tttagcgatg 600
acggtcggag ctactggtaa agaagttgag gcagtcgctc aacaattaaa acgtcaaaaa 660
acgatgaacc aagaccgagc cttggctatt ttaaatgatt taagaaaaca ataaaaaaac 720
agttcagcag aaattattct gctgaactgt tttttttcac attaggtagc cgtttcaggc 780
cacgagctca ggagttaaag aaatgacaat tgggattgat aaaattagtt tttttgtgcc 840
cccttattat attgatatga cggcactggc tgaagccaga aatgtagacc ctggaaaatt 900
tcatattggt attgggcaag accaaatggc ggtgaaccca atcagccaag atattgtgac 960
atttgcagcc aatgccgcag aagcgatctt gaccaaagaa gataaagagg ccattgatat 1020
ggtgattgtc gggactgagt ccagtatcga tgagtcaaaa gcggccgcag ttgtcttaca 1080
tcgtttaatg gggattcaac ctttcgctcg ctctttcgaa atcaaggaag cttgttacgg 1140
agcaacagca ggcttacagt tagctaagaa tcacgtagcc ttacatccag ataaaaaagt 1200
cttggttgta gcagcagata ttgcaaaata tggattaaat tctggcggtg agcctacaca 1260
aggagctggg gcggttgcaa tgttagttgc tagtgaaccg cgcatcttgg ctttaaaaga 1320
ggataatgtg atgctgacgc aagatatcta tgacttttgg cgtccaacag gccatccgta 1380
tcctatggtc gatggtcctt tgtcaaacga aacctacatc caatcttttg cccaagtctg 1440
ggatgaacat aaaaaaagaa ccggtcttga ttttgcagat tatgatgctt tagcgttcca 1500
tattccttac acaaaaatgg gcaaaaaagc cttattagca aaaatctccg accaaactga 1560
agcagaacag gaacgaattt tagcccgtta tgaagaaagc atcatctata gtcgtcgcgt 1620
aggaaacttg tatacgggtt cactttatct gggactcatt tcccttttag aaaatgcaac 1680
gactttaacc gcaggcaatc aaattgggtt attcagttat ggttctggtg ctgtcgctga 1740
atttttcact ggtgaattag tagctggtta tcaaaatcat ttacaaaaag aaactcattt 1800
agcactgcta gataatcgga cagaactttc tatcgctgaa tatgaagcca tgtttgcaga 1860
aactttagac acagatattg atcaaacgtt agaagatgaa ttaaaatata gtatttctgc 1920
tattaataat accgttcgct cttatcgaaa ctaactgcag cctcgacctg caggcatgca 1980
agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa 2040
cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc 2100
accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgagctta tcgatgataa 2160
gctgtcaaac atgagaatta caacttatat cgtatggggc tgacttcagg tgctacattt 2220
gaagagataa attgcactga aatctagaaa tattttatct gattaataag atgatcttct 2280
tgagatcgtt ttggtctgcg cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag 2340
ggcggttttt cgaaggttct ctgagctacc aactctttga accgaggtaa ctggcttgga 2400
ggagcgcagt caccaaaact tgtcctttca gtttagcctt aaccggcgca tgacttcaag 2460
actaactcct ctaaatcaat taccagtggc tgctgccagt ggtgcttttg catgtctttc 2520
cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcggact gaacgggggg 2580
ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg 2640
aatgagacaa acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag 2700
gagagcgcac gagggagccg ccaggggaaa cgcctggtat ctttatagtc ctgtcgggtt 2760
tcgccaccac tgatttgagc gtcagatttc gtgatgcttg tcaggggggc ggagcctatg 2820
gaaaaacggc tttgccgcgg ccctctcact tccctgttaa gtatcttcct ggcatcttcc 2880
aggaaatctc cgccccgttc gtaagccatt tccgctcgcc gcagtcgaac gaccgagcgt 2940
agcgagtcag tgagcgagga agcggaatat atcctgtatc acatattctg ctgacgcacc 3000
ggtgcagcct tttttctcct gccacatgaa gcacttcact gacaccctca tcagtgccaa 3060
catagtaagc cagtatacac tccgctagcg ctgatgtccg gcggtgcttt tgccgttacg 3120
caccaccccg tcagtagctg aacaggaggg acagctgata gaaacagaag ccactggagc 3180
acctcaaaaa caccatcata cactaaatca gtaagttggc agcatcaccc gacgcacttt 3240
gcgccgaata aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc 3300
ctgttgatac cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg 3360
taagaggttc caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt 3420
tatcgagatt ttcaggagct aaggaagcta aaatgagcca tattcaacgg gaaacgtctt 3480
gctctaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc 3540
gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc 3600
cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg 3660
tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta 3720
ctcctgatga tgcatggtta ctcaccactg cgatccccgg gaaaacagca ttccaggtat 3780
tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc 3840
ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg 3900
ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc 3960
gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac 4020
cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga 4080
aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg 4140
ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa 4200
aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt 4260
ttttctaaag tactgcgatg agtggcaggg cggggcgtaa tttttttaag gcagttattg 4320
gtgcccttaa acgcctggtg ctacgcctga ataagtgata ataagcggat gaatggcaga 4380
aattcgaaag caaattcgac ccggtcgtcg gttcagggca gggtcgttaa atagccgctt 4440
atgtctattg ctggtttacc ggtttattga ctaccggaag cagtgtgacc gtgtgcttct 4500
caaatgcctg aggccagttt gctcaggctc tccccgtgga ggtaataatt gacgatatga 4560
tcatttattc tgcctcccag agcctgataa aaacggttag cgcttcgtta atacagatgt 4620
aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca 4680
gggcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc 4740
tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat 4800
agtcatgccc cgcgcccacc ggaaggagct accggacagc ggtgcggact gttgtaactc 4860
agaataagaa atgaggccgc tcatggcgtt ccaatacgca aaccgcctct ccccgcgcgt 4920
tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag 4980
cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg 5040
cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc 5100
tatgaccatg attacgaatt cgagctcggt acccggggat cctacctgac gctttttatc 5160
gcaactctct actgtttctc catacccgtt tttttgggct agcaggagga attcaccatg 5220
gtaccaatga caaaaaaagt tggtgtcggt caggcacata gtaagataat tttaataggg 5280
gaacatgcgg tcgtttacgg ttatcctgcc atttccctgc ctcttttgga ggtggaggtg 5340
acctgtaagg tagttcctgc agagagtcct tggcgccttt atgaggagga taccttgtcc 5400
atggcggttt atgcctcact ggagtatttg aatatcacag aagcctgcat tcgttgtgag 5460
attgactcgg ctatccctga gaaacggggg atgggttcgt cagcggctat cagcatagcg 5520
gccattcgtg cagtatttga ctactatcag gctgatctgc ctcatgatgt actagaaatc 5580
ttggtcaatc gagctgaaat gattgcccat atgaatccta gtggtttgga tgctaagacc 5640
tgtcttagtg accaacctat tcgctttatc aagaacgtag gatttacaga acttgagatg 5700
gatttatccg cctatttggt gattgccgat acgggtgttt atggtcatac tcgtgaagcc 5760
atccaagtgg ttcaaaataa gggcaaggat gccctaccgt ttttgcatgc cttgggagaa 5820
ttaacccagc aagcagaagt tgcgatttca caaaaagatg ctgaaggact gggacaaatc 5880
ctcagtcaag cgcatttaca tttaaaagaa attggagtca gtagccctga ggcagacttt 5940
ttggttgaaa cgactcttag ccatggtgct ctgggtgcca agatgagcgg tggtgggcta 6000
ggaggttgta tcatagcctt ggtaaccaat ttgacacacg cacaagaact agcagaaaga 6060
ttagaagaga aaggagctgt tcagacatgg atagagagcc tgtaacagta cgttcctacg 6120
caaatattgc tattatcaaa tattggggaa agaaaaaaga aaaagagatg gtgcctgcta 6180
ctagcagtat ttctctaact ttggaaaata tgtatacaga gacgaccttg tcgcctttac 6240
cagccaatgt aacagctgac gaattttaca tcaatggtca gctacaaaat gaggtcgagc 6300
atgccaagat gagtaagatt attgaccgtt atcgtccagc tggtgagggc tttgtccgta 6360
tcgatactca aaacaatatg cctacggcag cgggcctgtc ctcaagttct agtggtttgt 6420
ccgccctggt caaggcttgt aatgcttatt tcaagcttgg attggataga agtcagttgg 6480
cacaggaagc caaatttgcc tcaggctctt cttctcggag tttttatgga ccactaggag 6540
cctgggataa ggatagtgga gaaatttacc ctgtagagac agacttgaaa ctagctatga 6600
ttatgttggt gctagaggac aagaaaaaac caatctctag ccgtgacggg atgaaacttt 6660
gtgtggaaac ctcgacgact tttgacgact gggttcgtca gtctgagaag gactatcagg 6720
atatgctgat ttatctcaag gaaaatgatt ttgccaagat tggagaatta acggagaaaa 6780
atgctctggc tatgcatgct acgacaaaga ctgctagtcc agccttttct tatctgacgg 6840
atgcctctta tgaggctatg gcctttgttc gccagcttcg tgagaaagga gaggcctgct 6900
actttaccat ggatgctggt cccaatgtta aggtcttctg tcaggagaaa gacttggagc 6960
atttgtcaga aattttcggt cagcgttatc gcttgattgt gtcaaaaaca aaggatttga 7020
gtcaagatga ttgctgttaa aacttgcgga aaactctatt gggcaggtga atatgctatt 7080
ttagagccag ggcagttagc tttgataaag gatattccca tctatatgag ggctgagatt 7140
gctttttctg acagctaccg tatctattca gatatgtttg atttcgcagt ggacttaagg 7200
cccaatcctg actacagctt gattcaagaa acgattgctt tgatgggaga cttcctcgct 7260
gttcgcggtc agaatttaag acctttttcc ctaaaaatct gtggcaaaat ggaacgagaa 7320
gggaaaaagt ttggtctagg ttctagtggc agcgtcgttg tcttggttgt caaggcttta 7380
ctggctctct ataatctttc ggttgatcag aatctcttgt tcaagctgac tagcgctgtc 7440
ttgctcaagc gaggagacaa tggttccatg ggcgaccttg cctgtattgt ggcagaggat 7500
ttggttcttt accagtcatt tgatcgccag aaggcggctg cttggttaga agaagaaaac 7560
ttggcgacag ttctggagcg tgattgggga ttttttatct cacaagtgaa accaacttta 7620
gaatgtgatt tcttagtggg atggaccaag gaagtggctg tatcgagtca catggtccag 7680
caaatcaagc aaaatatcaa tcaaaatttt ttaagttcct caaaagaaac ggtggtttct 7740
ttggtcgaag ccttggagca ggggaaagcc gaaaaagtta tcgagcaagt agaagtagcc 7800
agcaagcttt tagaaggctt gagtacagat atttacacgc ctttgcttag acagttgaaa 7860
gaagccagtc aagatttgca ggccgttgcc aagagtagtg gtgctggtgg tggtgactgt 7920
ggcatcgccc tgagttttga tgcgcaatct tctcgaaaca ctttaaaaaa tcgttgggcc 7980
gatctgggga ttgagctctt atatcaagaa aggataggac atgacgacaa atcgtaatct 8040
agccccggga ggagagaaat tatgcaaacg gaacacgtca ttttattgaa tgcacaggga 8100
gttcccacgg gtacgctgga aaagtatgcc gcacacacgg cagacacccg cttacatctc 8160
gcgttctcca gttggctgtt taatgccaaa ggacaattat tagttacccg ccgcgcactg 8220
agcaaaaaag catggcctgg cgtgtggact aactcggttt gtgggcaccc acaactggga 8280
gaaagcaacg aagacgcagt gatccgccgt tgccgttatg agcttggcgt ggaaattacg 8340
cctcctgaat ctatctatcc tgactttcgc taccgcgcca ccgatccgag tggcattgtg 8400
gaaaatgaag tgtgtccggt atttgccgca cgcaccacta gtgcgttaca gatcaatgat 8460
gatgaagtga tggattatca atggtgtgat ttagcagatg tattacacgg tattgatgcc 8520
acgccgtggg cgttcagtcc gtggatggtg atgcaggcga caaatcgcga agccagaaaa 8580
cgattatctg catttaccca gcttaaataa gcatgcacta gagtcgagga aacagaccat 8640
ggagttgaaa acagtagtta ttattgatgc attacgaaca ccaattggaa aatataaagg 8700
cagcttaagt caagtaagtg ccgtagactt aggaacacat gttacaacac aacttttaaa 8760
aagacattcc actatttctg aagaaattga tcaagtaatc tttggaaatg ttttacaagc 8820
tggaaatggc caaaatcccg cacgacaaat agcaataaac agcggtttat ctcatgaaat 8880
tcccgcaatg acagttaatg aggtctgcgg atcaggaatg aaggccgtta ttttggcgaa 8940
acaattgatt caattaggag aagcggaagt tttaattgct ggcgggattg agaatatgtc 9000
ccaagcacct aaattacaac gatttaatta cgaaacagaa agctatgatg cgcctttttc 9060
tagtatgatg tacgatgggt t 9081
<210> 81
<211> 8230
<212> DNA
<213> Artificial Sequence
<220>
<223> pSNA(-E)free
<400> 81
tttttttaag gcagttattg gtgcccttaa acgcctggtg ctacgcctga ataagtgata 60
ataagcggat gaatggcaga aattcgaaag caaattcgac ccggtcgtcg gttcagggca 120
gggtcgttaa atagccgctt atgtctattg ctggtttacc ggtttattga ctaccggaag 180
cagtgtgacc gtgtgcttct caaatgcctg aggccagttt gctcaggctc tccccgtgga 240
ggtaataatt gacgatatga tcatttattc tgcctcccag agcctgataa aaacggttag 300
cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag 360
atccggaaca taatggtgca gggcgcttgt ttcggcgtgg gtatggtggc aggccccgtg 420
gccgggggac tgttgggcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca 480
gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct accggacagc 540
ggtgcggact gttgtaactc agaataagaa atgaggccgc tcatggcgtt ccaatacgca 600
aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg 660
actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac 720
cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac 780
aatttcacac aggaaacagc tatgaccatg attacgaatt cgagctcggt acccggggat 840
cctacctgac gctttttatc gcaactctct actgtttctc catacccgtt tttttgggct 900
agcaggagga attcaccatg gtaccaatga caaaaaaagt tggtgtcggt caggcacata 960
gtaagataat tttaataggg gaacatgcgg tcgtttacgg ttatcctgcc atttccctgc 1020
ctcttttgga ggtggaggtg acctgtaagg tagttcctgc agagagtcct tggcgccttt 1080
atgaggagga taccttgtcc atggcggttt atgcctcact ggagtatttg aatatcacag 1140
aagcctgcat tcgttgtgag attgactcgg ctatccctga gaaacggggg atgggttcgt 1200
cagcggctat cagcatagcg gccattcgtg cagtatttga ctactatcag gctgatctgc 1260
ctcatgatgt actagaaatc ttggtcaatc gagctgaaat gattgcccat atgaatccta 1320
gtggtttgga tgctaagacc tgtcttagtg accaacctat tcgctttatc aagaacgtag 1380
gatttacaga acttgagatg gatttatccg cctatttggt gattgccgat acgggtgttt 1440
atggtcatac tcgtgaagcc atccaagtgg ttcaaaataa gggcaaggat gccctaccgt 1500
ttttgcatgc cttgggagaa ttaacccagc aagcagaagt tgcgatttca caaaaagatg 1560
ctgaaggact gggacaaatc ctcagtcaag cgcatttaca tttaaaagaa attggagtca 1620
gtagccctga ggcagacttt ttggttgaaa cgactcttag ccatggtgct ctgggtgcca 1680
agatgagcgg tggtgggcta ggaggttgta tcatagcctt ggtaaccaat ttgacacacg 1740
cacaagaact agcagaaaga ttagaagaga aaggagctgt tcagacatgg atagagagcc 1800
tgtaacagta cgttcctacg caaatattgc tattatcaaa tattggggaa agaaaaaaga 1860
aaaagagatg gtgcctgcta ctagcagtat ttctctaact ttggaaaata tgtatacaga 1920
gacgaccttg tcgcctttac cagccaatgt aacagctgac gaattttaca tcaatggtca 1980
gctacaaaat gaggtcgagc atgccaagat gagtaagatt attgaccgtt atcgtccagc 2040
tggtgagggc tttgtccgta tcgatactca aaacaatatg cctacggcag cgggcctgtc 2100
ctcaagttct agtggtttgt ccgccctggt caaggcttgt aatgcttatt tcaagcttgg 2160
attggataga agtcagttgg cacaggaagc caaatttgcc tcaggctctt cttctcggag 2220
tttttatgga ccactaggag cctgggataa ggatagtgga gaaatttacc ctgtagagac 2280
agacttgaaa ctagctatga ttatgttggt gctagaggac aagaaaaaac caatctctag 2340
ccgtgacggg atgaaacttt gtgtggaaac ctcgacgact tttgacgact gggttcgtca 2400
gtctgagaag gactatcagg atatgctgat ttatctcaag gaaaatgatt ttgccaagat 2460
tggagaatta acggagaaaa atgctctggc tatgcatgct acgacaaaga ctgctagtcc 2520
agccttttct tatctgacgg atgcctctta tgaggctatg gcctttgttc gccagcttcg 2580
tgagaaagga gaggcctgct actttaccat ggatgctggt cccaatgtta aggtcttctg 2640
tcaggagaaa gacttggagc atttgtcaga aattttcggt cagcgttatc gcttgattgt 2700
gtcaaaaaca aaggatttga gtcaagatga ttgctgttaa aacttgcgga aaactctatt 2760
gggcaggtga atatgctatt ttagagccag ggcagttagc tttgataaag gatattccca 2820
tctatatgag ggctgagatt gctttttctg acagctaccg tatctattca gatatgtttg 2880
atttcgcagt ggacttaagg cccaatcctg actacagctt gattcaagaa acgattgctt 2940
tgatgggaga cttcctcgct gttcgcggtc agaatttaag acctttttcc ctaaaaatct 3000
gtggcaaaat ggaacgagaa gggaaaaagt ttggtctagg ttctagtggc agcgtcgttg 3060
tcttggttgt caaggcttta ctggctctct ataatctttc ggttgatcag aatctcttgt 3120
tcaagctgac tagcgctgtc ttgctcaagc gaggagacaa tggttccatg ggcgaccttg 3180
cctgtattgt ggcagaggat ttggttcttt accagtcatt tgatcgccag aaggcggctg 3240
cttggttaga agaagaaaac ttggcgacag ttctggagcg tgattgggga ttttttatct 3300
cacaagtgaa accaacttta gaatgtgatt tcttagtggg atggaccaag gaagtggctg 3360
tatcgagtca catggtccag caaatcaagc aaaatatcaa tcaaaatttt ttaagttcct 3420
caaaagaaac ggtggtttct ttggtcgaag ccttggagca ggggaaagcc gaaaaagtta 3480
tcgagcaagt agaagtagcc agcaagcttt tagaaggctt gagtacagat atttacacgc 3540
ctttgcttag acagttgaaa gaagccagtc aagatttgca ggccgttgcc aagagtagtg 3600
gtgctggtgg tggtgactgt ggcatcgccc tgagttttga tgcgcaatct tctcgaaaca 3660
ctttaaaaaa tcgttgggcc gatctgggga ttgagctctt atatcaagaa aggataggac 3720
atgacgacaa atcgtaatct agccccggga ggagagaaat tatgcaaacg gaacacgtca 3780
ttttattgaa tgcacaggga gttcccacgg gtacgctgga aaagtatgcc gcacacacgg 3840
cagacacccg cttacatctc gcgttctcca gttggctgtt taatgccaaa ggacaattat 3900
tagttacccg ccgcgcactg agcaaaaaag catggcctgg cgtgtggact aactcggttt 3960
gtgggcaccc acaactggga gaaagcaacg aagacgcagt gatccgccgt tgccgttatg 4020
agcttggcgt ggaaattacg cctcctgaat ctatctatcc tgactttcgc taccgcgcca 4080
ccgatccgag tggcattgtg gaaaatgaag tgtgtccggt atttgccgca cgcaccacta 4140
gtgcgttaca gatcaatgat gatgaagtga tggattatca atggtgtgat ttagcagatg 4200
tattacacgg tattgatgcc acgccgtggg cgttcagtcc gtggatggtg atgcaggcga 4260
caaatcgcga agccagaaaa cgattatctg catttaccca gcttaaataa gcatgcacta 4320
gagtcgagga aacagaccat ggagttgaaa acagtagtta ttattgatgc attacgaaca 4380
ccaattggaa aatataaagg cagcttaagt caagtaagtg ccgtagactt aggaacacat 4440
gttacaacac aacttttaaa aagacattcc actatttctg aagaaattga tcaagtaatc 4500
tttggaaatg ttttacaagc tggaaatggc caaaatcccg cacgacaaat agcaataaac 4560
agcggtttat ctcatgaaat tcccgcaatg acagttaatg aggtctgcgg atcaggaatg 4620
aaggccgtta ttttggcgaa acaattgatt caattaggag aagcggaagt tttaattgct 4680
ggcgggattg agaatatgtc ccaagcacct aaattacaac gatttaatta cgaaacagaa 4740
agctatgatg cgcctttttc tagtatgatg tacgatgggt taacgctatg ttggaaggtg 4800
tggccgagtt gttccgtgaa tggtttgcgg agcaaaagat tttattcagt attttaagta 4860
attatgccac ggagtcggtt gttacgatga aaacggctat tccagtttca cgtttaagta 4920
aggggagcaa tggccgggaa attgctgaaa aaattgtttt agcttcacgc tatgcttcat 4980
tagatcctta tcgggcagtc acgcataaca aagggatcat gaatggcatt gaagctgtcg 5040
ttttagctac aggaaatgat acacgcgctg ttagcgcttc ttgtcatgct tttgcggtga 5100
aggaaggtcg ctaccaaggt ttgactagtt ggacgctgga tggcgaacaa ctaattggtg 5160
aaatttcagt tccgcttgcg ttagccacgg ttggcggtgc cacaaaagtc ttacctaaat 5220
ctcaagcagc tgctgatttg ttagcagtga cggatgcaaa agaactaagt cgagtagtag 5280
cggctgttgg tttggcacaa aatttagcgg cgttacgggc cttagtctct gaaggaattc 5340
aaaaaggaca catggctcta caagcacgtt ctttagcgat gacggtcgga gctactggta 5400
aagaagttga ggcagtcgct caacaattaa aacgtcaaaa aacgatgaac caagaccgag 5460
ccttggctat tttaaatgat ttaagaaaac aataaaaaaa cagttcagca gaaattattc 5520
tgctgaactg ttttttttca cattaggtag ccgtttcagg ccacgagctc aggagttaaa 5580
gaaatgacaa ttgggattga taaaattagt ttttttgtgc ccccttatta tattgatatg 5640
acggcactgg ctgaagccag aaatgtagac cctggaaaat ttcatattgg tattgggcaa 5700
gaccaaatgg cggtgaaccc aatcagccaa gatattgtga catttgcagc caatgccgca 5760
gaagcgatct tgaccaaaga agataaagag gccattgata tggtgattgt cgggactgag 5820
tccagtatcg atgagtcaaa agcggccgca gttgtcttac atcgtttaat ggggattcaa 5880
cctttcgctc gctctttcga aatcaaggaa gcttgttacg gagcaacagc aggcttacag 5940
ttagctaaga atcacgtagc cttacatcca gataaaaaag tcttggttgt agcagcagat 6000
attgcaaaat atggattaaa ttctggcggt gagcctacac aaggagctgg ggcggttgca 6060
atgttagttg ctagtgaacc gcgcatcttg gctttaaaag aggataatgt gatgctgacg 6120
caagatatct atgacttttg gcgtccaaca ggccatccgt atcctatggt cgatggtcct 6180
ttgtcaaacg aaacctacat ccaatctttt gcccaagtct gggatgaaca taaaaaaaga 6240
accggtcttg attttgcaga ttatgatgct ttagcgttcc atattcctta cacaaaaatg 6300
ggcaaaaaag ccttattagc aaaaatctcc gaccaaactg aagcagaaca ggaacgaatt 6360
ttagcccgtt atgaagaaag catcatctat agtcgtcgcg taggaaactt gtatacgggt 6420
tcactttatc tgggactcat ttccctttta gaaaatgcaa cgactttaac cgcaggcaat 6480
caaattgggt tattcagtta tggttctggt gctgtcgctg aatttttcac tggtgaatta 6540
gtagctggtt atcaaaatca tttacaaaaa gaaactcatt tagcactgct agataatcgg 6600
acagaacttt ctatcgctga atatgaagcc atgtttgcag aaactttaga cacagatatt 6660
gatcaaacgt tagaagatga attaaaatat agtatttctg ctattaataa taccgttcgc 6720
tcttatcgaa actaactgca gcctcgacct gcaggcatgc aagcttggca ctggccgtcg 6780
ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac 6840
atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac 6900
agttgcgcag cctgaatggc gaatgagctt atcgatgata agctgtcaaa catgagaatt 6960
acaacttata tcgtatgggg ctgacttcag gtgctacatt tgaagagata aattgcactg 7020
aaatctagaa atattttatc tgattaataa gatgatcttc ttgagatcgt tttggtctgc 7080
gcgtaatctc ttgctctgaa aacgaaaaaa ccgccttgca gggcggtttt tcgaaggttc 7140
tctgagctac caactctttg aaccgaggta actggcttgg aggagcgcag tcaccaaaac 7200
ttgtcctttc agtttagcct taaccggcgc atgacttcaa gactaactcc tctaaatcaa 7260
ttaccagtgg ctgctgccag tggtgctttt gcatgtcttt ccgggttgga ctcaagacga 7320
tagttaccgg ataaggcgca gcggtcggac tgaacggggg gttcgtgcat acagtccagc 7380
ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg gaatgagaca aacgcggcca 7440
taacagcgga atgacaccgg taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc 7500
gccaggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacca ctgatttgag 7560
cgtcagattt cgtgatgctt gtcagggggg cggagcctat ggaaaaacgg ctttgccgcg 7620
gccctctcac ttccctgtta agtatcttcc tggcatcttc caggaaatct ccgccccgtt 7680
cgtaagccat ttccgctcgc cgcagtcgaa cgaccgagcg tagcgagtca gtgagcgagg 7740
aagcggaata tatcctgtat cacatattct gctgacgcac cggtgcagcc ttttttctcc 7800
tgccacatga agcacttcac tgacaccctc atcagtgcca acatagtaag ccagtataca 7860
ctccgctagc gctgatgtcc ggcggtgctt ttgccgttac gcaccacccc gtcagtagct 7920
gaacaggagg gacagctgat agaaacagaa gccactggag cacctcaaaa acaccatcat 7980
acactaaatc agtaagttgg cagcatcacc cgacgcactt tgcgccgaat aaatacctgt 8040
gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata ccgggaagcc 8100
ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt ccaactttca 8160
ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat tttcaggagc 8220
taaggaagct 8230
<210> 82
<211> 6678
<212> DNA
<213> Artificial Sequence
<220>
<223> pTEFAmvaE
<400> 82
gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc 60
ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc 120
gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc 180
tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga 240
taacaatttc acacaggaaa cagaccatgg ggctgcagga attcgatgcc atggagttga 300
aaacagtagt tattattgat gcattacgaa caccaattgg aaaatataaa ggcagcttaa 360
gtcaagtaag tgccgtagac ttaggaacac atgttacaac acaactttta aaaagacatt 420
ccactatttc tgaagaaatt gatcaagtaa tctttggaaa tgttttacaa gctggaaatg 480
gccaaaatcc cgcacgacaa atagcaataa acagcggttt gtctcatgaa attcccgcaa 540
tgacggttaa tgaggtctgc ggatcaggaa tgaaggccgt tattttggcg aaacaattga 600
ttcaattagg agaagcggaa gttttaattg ctggcgggat tgagaatatg tcccaagcac 660
ctaaattaca acgttttaat tacgaaacag aaagctacga tgcgcctttt tctagtatga 720
tgtatgatgg attaacggat gcctttagtg gtcaggcaat gggcttaact gctgaaaatg 780
tggccgaaaa gtatcatgta actagagaag agcaagatca attttctgta cattcacaat 840
taaaagcagc tcaagcacaa gcagaaggga tattcgctga cgaaatagcc ccattagaag 900
tatcaggaac gcttgtggag aaagatgaag ggattcgccc taattcgagc gttgagaagc 960
taggaacgct taaaacagtt tttaaagaag acggtactgt aacagcaggg aatgcatcaa 1020
ccattaatga tggggcttct gctttgatta ttgcttcaca agaatatgcc gaagcacacg 1080
gtcttcctta tttagctatt attcgagaca gtgtggaagt cggtattgat ccagcctata 1140
tgggaatttc gccgattaaa gccattcaaa aactgttagc gcgcaatcaa cttactacgg 1200
aagaaattga tctgtatgaa atcaacgaag catttgcagc aacttcaatc gtggtccaaa 1260
gagaactggc tttaccagag gaaaaggtca acatttatgg tggcggtatt tcattaggtc 1320
atgcgattgg tgccacaggt gctcgtttat taacgagttt aagttatcaa ttaaatcaaa 1380
aagaaaagaa atatggagtg gcttctttat gtatcggcgg tggcttagga ctcgctatgc 1440
tactagagag acctcagcaa aaaaaaaaca gccgatttta tcaaatgagt cctgaggaac 1500
gcctggcttc tcttcttaat gaaggccaga tttctgctga tacaaaaaaa gaatttgaaa 1560
atacggcttt atcttcgcag attgccaatc atatgattga aaatcaaatc agtgaaacag 1620
aagtgccgat gggcgttggc ttacatttaa cagtggacga aactgattat ttggtaccaa 1680
tggcgacaga agagccctca gttattgcgg ctttgagtaa tggtgcaaaa atagcacaag 1740
gatttaaaac agtgaatcaa caacgcttaa tgcgtggaca aatcgttttt tacgatgttg 1800
cagatcccga gtcattgatt gataaactac aagtaagaga agcggaagtt tttcaacaag 1860
cagagttaag ttatccatct atcgttaaac ggggcggcgg cttaagagat ttgcaatatc 1920
gtacttttga tgaatcattt gtatctgtcg actttttagt agatgttaag gatgcaatgg 1980
gggcaaatat cgttaacgct atgttggaag gtgtggccga gttgttccgt gaatggtttg 2040
cggagcaaaa gattttattc agtattttaa gtaattatgc cacggagtcg gttgttacga 2100
tgaaaacggc tattccagtt tcacgtttaa gtaaggggag caatggccgg gaaattgctg 2160
aaaaaattgt tttagcttca cgctatgctt cattagatcc ttatcgggca gtcacgcata 2220
acaaaggaat catgaatggc attgaagctg tagttttagc tacaggaaat gatacacgcg 2280
ctgttagcgc ttcttgtcat gcttttgcgg tgaaggaagg tcgctaccaa ggcttgacta 2340
gttggacgct ggatggcgaa caactaattg gtgaaatttc agttccgctt gctttagcca 2400
cggttggcgg tgccacaaaa gtcttaccta aatctcaagc agctgctgat ttgttagcag 2460
tgacggatgc aaaagaacta agtcgagtag tagcggctgt tggtttggca caaaatttag 2520
cggcgttacg ggccttagtc tctgaaggaa ttcaaaaagg acacatggct ctacaagcac 2580
gttctttagc gatgacggtc ggagctactg gtaaagaagt tgaggcagtc gctcaacaat 2640
taaaacgtca aaaaacgatg aaccaagacc gagccatggc tattttaaat gatttaagaa 2700
aacaataaaa aaacagttca gcagaaatta ttctgctgaa ctattttttt tcacattagg 2760
tagccgtttc aggccacgag ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat 2820
gcaagcttgg ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag 2880
aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac 2940
ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc 3000
cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac 3060
tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg 3120
ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg 3180
ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg 3240
tttctacaaa ctctttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 3300
gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 3360
atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 3420
cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 3480
tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 3540
caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg 3600
ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 3660
cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 3720
taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 3780
agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 3840
cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct acagcaatgg 3900
caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 3960
taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 4020
ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 4080
cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 4140
aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 4200
attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 4260
tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 4320
aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 4380
gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 4440
cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 4500
gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca 4560
agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 4620
ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 4680
cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 4740
acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 4800
gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 4860
ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 4920
agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 4980
cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 5040
tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 5100
gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc 5160
ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta 5220
caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg 5280
ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct 5340
gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag 5400
gttttcaccg tcatcaccga aacgcgcgag gcagcagatc aattcgcgcg cgaaggcgaa 5460
gcggcatgca tttacgttga caccatcgaa tggtgcaaaa cctttcgcgg tatggcatga 5520
tagcgcccgg aagagagtca attcagggtg gtgaatgtga aaccagtaac gttatacgat 5580
gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc 5640
cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt 5700
cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc 5760
tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat 5820
caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa 5880
gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg 5940
gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt 6000
gatgtctctg accagacacc catcaacagt attattttct cccatgaaga cggtacgcga 6060
ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca 6120
ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat 6180
caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa 6240
accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag 6300
atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc 6360
tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc 6420
atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct 6480
cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc 6540
accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 6600
ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag 6660
ttagcgcgaa ttgatctg 6678
<210> 83
<211> 13288
<212> DNA
<213> Artificial Sequence
<220>
<223> pT-DHBSRYbbO
<400> 83
gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc 60
ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc 120
gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc 180
tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga 240
taacaatttc acacaggaaa cagaccatgg aattctacgt actagtgaat tcaggaggta 300
ataaatatgg gtctgatgct gattgattgg tgtgcactgg ctctggttgt tttcattggc 360
ctgccgcacg gcgcgctgga tgctgccatt tctttttcta tgatctcttc tgcaaaacgc 420
attgctcgtc tggctggtat tctgctgatc tatctgctgc tggcgaccgc gttcttcctg 480
atctggtatc agctgccagc gtttagcctg ctgatcttcc tgctgatctc cattatccac 540
tttggtatgg cagacttcaa cgcgtcccca agcaaactga aatggccgca tatcatcgcc 600
cacggcggtg ttgttactgt ttggctgccg ctgatccaga aaaacgaagt aactaaactg 660
tttagcatcc tgactaacgg tccgactccg atcctgtggg acatcctgct gattttcttc 720
ctgtgttggt ctattggcgt gtgtctgcac acgtacgaaa ccctgcgctc taaacattac 780
aacatcgcct ttgaactgat cggtctgatt ttcctggcgt ggtatgcgcc gcctctggtt 840
acgtttgcca cttacttctg cttcattcat tcccgtcgcc acttctcctt tgtgtggaag 900
cagctgcaac acatgtcttc caaaaagatg atgattggca gcgcgattat cctgtcctgt 960
acctcttggc tgatcggcgg tggtatctat ttcttcctga actccaaaat gatcgcctct 1020
gaggctgcgc tgcagactgt gttcatcggt ctggcggcac tgaccgtgcc gcacatgatt 1080
ctgatcgact tcatcttccg tccgcactct tcccgtatca aaatcaaaaa ctaattaatt 1140
aaaggaggta ataatatgac tcataaagca acggagatcc tgacaggtaa agttatgcaa 1200
aaatcggtct taattaccgg atgttccagt ggaattggcc tggaaagcgc gctcgaatta 1260
aaacgccagg gttttcatgt gctggcaggt tgccggaaac cggatgatgt tgagcgcatg 1320
aacagcatgg gatttaccgg cgtgttgatc gatctggatt caccagaaag tgttgatcgc 1380
gcagccgacg aggtgatcgc cctgaccgat aattgtctgt atgggatctt taacaatgcc 1440
ggattcggca tgtatggccc cctttccacc atcagccgtg cgcagatgga acagcagttt 1500
tccgccaact ttttcggcgc acaccagctc accatgcgcc tgttacccgc gatgttaccg 1560
cacggtgaag ggcgtattgt gatgacatca tcggtgatgg gattaatctc cacgccgggt 1620
cgtggcgctt acgcggccag taaatatgcg ctggaggcgt ggtcagatgc actgcgcatg 1680
gagctgcgcc acagcggaat taaagtcagc ctgatcgaac ccggtcccat tcgtactcgc 1740
ttcaccgaca acgtcaacca gacgcaaagt gataaaccag tcgaaaatcc cggcatcgcc 1800
gcccgcttta cgttgggacc ggaagcggtg gtggacaaag tacgccatgc ttttattagc 1860
gagaagccga agatgcgcta tccggtgacg ctggtgacct gggcggtaat ggtgcttaag 1920
cgcctgctgc cggggcgcgt gatggacaaa atattgcagg ggtaactagt aggaggtaat 1980
aaatatggtg agtggcagta aagcgggcgt ttcgcctcat cgcgaaatag aagtaatgag 2040
acaatccatt gacgatcacc tggctggcct gttacctgaa accgacagcc aggatatcgt 2100
cagccttgcg atgcgtgaag gcgtcatggc acccggtaaa cggatccgtc cgctgctgat 2160
gctgctggcc gcccgcgacc tccgctacca gggcagtatg cctacgctgc tcgatctcgc 2220
ctgcgccgtt gaactgaccc ataccgcgtc gctgatgctc gacgacatgc cctgcatgga 2280
caacgccgag ctgcgccgcg gtcagcccac tacccacaaa aaatttggtg agagcgtggc 2340
gatccttgcc tccgttgggc tgctctctaa agcctttggt ctgatcgccg ccaccggcga 2400
tctgccgggg gagaggcgtg cccaggcggt caacgagctc tctaccgccg tgggcgtgca 2460
gggcctggta ctggggcagt ttcgcgatct taacgatgcc gccctcgacc gtacccctga 2520
cgctatcctc agcaccaacc acctcaagac cggcattctg ttcagcgcga tgctgcagat 2580
cgtcgccatt gcttccgcct cgtcgccgag cacgcgagag acgctgcacg ccttcgccct 2640
cgacttcggc caggcgtttc aactgctgga cgatctgcgt gacgatcacc cggaaaccgg 2700
taaagatcgc aataaggacg cgggaaaatc gacgctggtc aaccggctgg gcgcagacgc 2760
ggcccggcaa aagctgcgcg agcatattga ttccgccgac aaacacctca cttttgcctg 2820
tccgcagggc ggcgccatcc gacagtttat gcatctgtgg tttggccatc accttgccga 2880
ctggtcaccg gtcatgaaaa tcgcctgata actcgaggag gtataaagga tgaaaaaaac 2940
cgttgtgatt ggcgcaggct ttggtggcct ggcgctggcg attcgcctgc aggcggcagg 3000
gatcccaacc gtactgctgg agcagcggga caagcccggc ggtcgggcct acgtctggca 3060
tgaccagggc tttacctttg acgccgggcc gacggtgatc accgatccta ccgcgcttga 3120
ggcgctgttc accctggccg gcaggcgcat ggaggattac gtcaggctgc tgccggtaaa 3180
acccttctac cgactctgct gggagtccgg gaagaccctc gactatgcta acgacagcgc 3240
cgagcttgag gcgcagatta cccagttcaa cccccgcgac gtcgagggct accggcgctt 3300
tctggcttac tcccaggcgg tattccagga gggatatttg cgcctcggca gcgtgccgtt 3360
cctctctttt cgcgacatgc tgcgcgccgg gccgcagctg cttaagctcc aggcgtggca 3420
gagcgtctac cagtcggttt cgcgctttat tgaggatgag catctgcggc aggccttctc 3480
gttccactcc ctgctggtag gcggcaaccc cttcaccacc tcgtccatct acaccctgat 3540
ccacgccctt gagcgggagt ggggggtctg gttccctgag ggcggcaccg gggcgctggt 3600
gaacggcatg gtgaagctgt ttaccgatct gggcggggag atcgaactca acgcccgggt 3660
cgaagagctg gtggtggccg ataaccgcgt aagccaggtc cggctggcgg atggtcggat 3720
ctttgacacc gacgccgtag cctcgaacgc tgacgtggtg aacacctata aaaagctgct 3780
cggccaccat ccggtggggc agaagcgggc ggcagcgctg gagcgcaaga gcatgagcaa 3840
ctcgctgttt gtgctctact tcggcctgaa ccagcctcat tcccagctgg cgcaccatac 3900
catctgtttt ggtccccgct accgggagct gatcgacgag atctttaccg gcagcgcgct 3960
ggcggatgac ttctcgctct acctgcactc gccctgcgtg accgatccct cgctcgcgcc 4020
tcccggctgc gccagcttct acgtgctggc cccggtgccg catcttggca acgcgccgct 4080
ggactgggcg caggaggggc cgaagctgcg cgaccgcatc tttgactacc ttgaagagcg 4140
ctatatgccc ggcctgcgta gccagctggt gacccagcgg atctttaccc cggcagactt 4200
ccacgacacg ctggatgcgc atctgggatc ggccttctcc atcgagccgc tgctgaccca 4260
aagcgcctgg ttccgcccgc acaaccgcga cagcgacatt gccaacctct acctggtggg 4320
cgcaggtact caccctgggg cgggcattcc tggcgtagtg gcctcggcga aagccaccgc 4380
cagcctgatg attgaggatc tgcaatgagc caaccgccgc tgcttgacca cgccacgcag 4440
accatggcca acggctcgaa aagttttgcc accgctgcga agctgttcga cccggccacc 4500
cgccgtagcg tgctgatgct ctacacctgg tgccgccact gcgatgacgt cattgacgac 4560
cagacccacg gcttcgccag cgaggccgcg gcggaggagg aggccaccca gcgcctggcc 4620
cggctgcgca cgctgaccct ggcggcgttt gaaggggccg agatgcagga tccggccttc 4680
gctgcctttc aggaggtggc gctgacccac ggtattacgc cccgcatggc gctcgatcac 4740
ctcgacggct ttgcgatgga cgtggctcag acccgctatg tcacctttga ggatacgctg 4800
cgctactgct atcacgtggc gggcgtggtg ggtctgatga tggccagggt gatgggcgtg 4860
cgggatgagc gggtgctgga tcgcgcctgc gatctggggc tggccttcca gctgacgaat 4920
atcgcccggg atattattga cgatgcggct attgaccgct gctatctgcc cgccgagtgg 4980
ctgcaggatg ccgggctgac cccggagaac tatgccgcgc gggagaatcg ggccgcgctg 5040
gcgcgggtgg cggagcggct tattgatgcc gcagagccgt actacatctc ctcccaggcc 5100
gggctacacg atctgccgcc gcgctgcgcc tgggcgatcg ccaccgcccg cagcgtctac 5160
cgggagatcg gtattaaggt aaaagcggcg ggaggcagcg cctgggatcg ccgccagcac 5220
accagcaaag gtgaaaaaat tgccatgctg atggcggcac cggggcaggt tattcgggcg 5280
aagacgacga gggtgacgcc gcgtccggcc ggtctttggc agcgtcccgt ttagtaatct 5340
agaggaggta ataaaatatg cttcgttcgt tgctcagagg cctcacgcat atcccccgcg 5400
tgaactccgc ccagcagccc agctgtgcac acgcgcgact ccagtttaag ctcaggagca 5460
tgcagatgac gctcatgcag cccagcatct cagccaatct gtcgcgcgcc gaggaccgca 5520
cagaccacat gaggggtgca agcacctggg caggcgggca gtcgcaggat gagctgatgc 5580
tgaaggacga gtgcatcttg gtggatgttg aggacaacat cacaggccat gccagcaagc 5640
tggagtgtca caagttccta ccacatcagc ctgcaggcct gctgcaccgg gccttctctg 5700
tgttcctgtt tgacgatcag gggcgactgc tgctgcaaca gcgtgcacgc tcaaaaatca 5760
ccttcccaag tgtgtggacg aacacctgct gcagccaccc tttacatggg cagaccccag 5820
atgaggtgga ccaactaagc caggtggccg acggaacagt acctggcgca aaggctgctg 5880
ccatccgcaa gttggagcac gagctgggga taccagcgca ccagctgccg gcaagcgcgt 5940
ttcgcttcct cacgcgtttg cactactgtg ccgcggacgt gcagccagct gcgacacaat 6000
cagcgctctg gggcgagcac gaaatggact acatcttgtt catccgggcc aacgtcacct 6060
tggcgcccaa ccctgacgag gtggacgaag tcaggtacgt gacgcaagag gagctgcggc 6120
agatgatgca gccggacaac gggctgcaat ggtcgccgtg gtttcgcatc atcgccgcgc 6180
gcttccttga gcgttggtgg gctgacctgg acgcggccct aaacactgac aaacacgagg 6240
attggggaac ggtgcatcac atcaacgaag cgtgataagc ggccgcgtcg acaggaggag 6300
cggctatgca accgcattat gatctgattc tcgtgggggc tggactcgcg aatggcctta 6360
tcgccctgcg tcttcagcag cagcaacctg atatgcgtat tttgcttatc gacgccgcac 6420
cccaggcggg cgggaatcat acgtggtcat ttcaccacga tgatttgact gagagccaac 6480
atcgttggat agctccgctg gtggttcatc actggcccga ctatcaggta cgctttccca 6540
cacgccgtcg taagctgaac agcggctact tttgtattac ttctcagcgt ttcgctgagg 6600
ttttacagcg acagtttggc ccgcacttgt ggatggatac cgcggtcgca gaggttaatg 6660
cggaatctgt tcggttgaaa aagggtcagg ttatcggtgc ccgcgcggtg attgacgggc 6720
ggggttatgc ggcaaattca gcactgagcg tgggcttcca ggcgtttatt ggccaggaat 6780
ggcgattgag ccacccgcat ggtttatcgt ctcccattat catggatgcc acggtcgatc 6840
agcaaaatgg ttatcgcttc gtgtacagcc tgccgctctc gccgaccaga ttgttaattg 6900
aagacacgca ctatattgat aatgcgacat tagatcctga atgcgcgcgg caaaatattt 6960
gcgactatgc cgcgcaacag ggttggcagc ttcagacact gctgcgagaa gaacagggcg 7020
ccttacccat tactctgtcg ggcaatgccg acgcattctg gcagcagcgc cccctggcct 7080
gtagtggatt acgtgccggt ctgttccatc ctaccaccgg ctattcactg ccgctggcgg 7140
ttgccgtggc cgaccgcctg agtgcacttg atgtctttac gtcggcctca attcaccatg 7200
ccattacgca ttttgcccgc gagcgctggc agcagcaggg ctttttccgc atgctgaatc 7260
gcatgctgtt tttagccgga cccgccgatt cacgctggcg ggttatgcag cgtttttatg 7320
gtttacctga agatttaatt gcccgttttt atgcgggaaa actcacgctg accgatcggc 7380
tacgtattct gagcggcaag ccgcctgttc cggtattagc agcattgcaa gccattatga 7440
cgactcatcg ttaactgcag gcatgcaagc tggggatcct acctgacgct ttttatcgca 7500
actctctact gtttctccat acccgttttt ttgggctagc aggaggaatt caccatggaa 7560
ttcatgagtt ttgatattgc caaatacccg accctggcac tggtcgactc cacccaggag 7620
ttacgactgt tgccgaaaga gagtttaccg aaactctgcg acgaactgcg ccgctattta 7680
ctcgacagcg tgagccgttc cagcgggcac ttcgcctccg ggctgggcac ggtcgaactg 7740
accgtggcgc tgcactatgt ctacaacacc ccgtttgacc aattgatttg ggatgtgggg 7800
catcaggctt atccgcataa aattttgacc ggacgccgcg acaaaatcgg caccatccgt 7860
cagaaaggcg gtctgcaccc gttcccgtgg cgcggcgaaa gcgaatatga cgtattaagc 7920
gtcgggcatt catcaacctc catcagtgcc ggaattggta ttgcggttgc tgccgaaaaa 7980
gaaggcaaaa atcgccgcac cgtctgtgtc attggcgatg gcgcgattac cgcaggcatg 8040
gcgtttgaag cgatgaatca cgcgggcgat atccgtcctg atatgctggt gattctcaac 8100
gacaatgaaa tgtcgatttc cgaaaatgtc ggcgcgctca acaaccatct ggcacagctg 8160
ctttccggta agctttactc ttcactgcgc gaaggcggga aaaaagtttt ctctggcgtg 8220
ccgccaatta aagagctgct caaacgcacc gaagaacata ttaaaggcat ggtagtgcct 8280
ggcacgttgt ttgaagagct gggctttaac tacatcggcc cggtggacgg tcacgatgtg 8340
ctggggctta tcaccacgct aaagaacatg cgcgacctga aaggcccgca gttcctgcat 8400
atcatgacca aaaaaggtcg tggttatgaa ccggcagaaa aagacccgat cactttccac 8460
gccgtgccta aatttgatcc ctccagcggt tgtttgccga aaagtagcgg cggtttgccg 8520
agctattcaa aaatctttgg cgactggttg tgcgaaacgg cagcgaaaga caacaagctg 8580
atggcgatta ctccggcgat gcgtgaaggt tccggcatgg tcgagttttc acgtaaattc 8640
ccggatcgct acttcgacgt ggcaattgcc gagcaacacg cggtgacctt tgctgcgggt 8700
ctggcgattg gtgggtacaa acccattgtc gcgatttact ccactttcct gcaacgcgcc 8760
tatgatcagg tgctgcatga cgtggcgatt caaaagcttc cggtcctgtt cgccatcgac 8820
cgcgcgggca ttgttggtgc tgacggtcaa acccatcagg gtgcttttga tctctcttac 8880
ctgcgctgca taccggaaat ggtcattatg accccgagcg atgaaaacga atgtcgccag 8940
atgctctata ccggctatca ctataacgat ggcccgtcag cggtgcgcta cccgcgtggc 9000
aacgcggtcg gcgtggaact gacgccgctg gaaaaactac caattggcaa aggcattgtg 9060
aagcgtcgtg gcgagaaact ggcgatcctt aactttggta cgctgatgcc agaagcggcg 9120
aaagtcgccg aatcgctgaa cgccacgctg gtcgatatgc gttttgtgaa accgcttgat 9180
gaagcgttaa ttctggaaat ggccgccagc catgaagcgc tggtcaccgt agaagaaaac 9240
gccattatgg gcggcgcagg cagcggcgtg aacgaagtgc tgatggccca tcgtaaacca 9300
gtacccgtgc tgaacattgg cctgccggac ttctttattc cgcaaggaac tcaggaagaa 9360
atgcgcgccg aactcggcct cgatgccgct ggtatggaag ccaaaatcaa ggcctggctg 9420
gcataaggta cccagcttgg ctgttttggc ggatgagaga agattttcag cctgatacag 9480
attaaatcag aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg 9540
gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt 9600
gtggggtctc cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca 9660
gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag 9720
gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc 9780
aggacgcccg ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg 9840
cctttttgcg tttctacaaa ctctttttgt ttatttttct aaatacattc aaatatgtat 9900
ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg 9960
agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt 10020
tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga 10080
gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa 10140
gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt 10200
gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt 10260
gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc 10320
agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga 10380
ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat 10440
cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct 10500
acagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc 10560
cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg 10620
gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc 10680
ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg 10740
acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca 10800
ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta 10860
aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 10920
aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 10980
ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 11040
ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 11100
actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc 11160
caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 11220
gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 11280
ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 11340
cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 11400
cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 11460
acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 11520
ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 11580
gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 11640
tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat 11700
accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag 11760
cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc 11820
actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc 11880
tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac 11940
gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca 12000
tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagcagatc aattcgcgcg 12060
cgaaggcgaa gcggcatgca tttacgttga caccatcgaa tggtgcaaaa cctttcgcgg 12120
tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatgtga aaccagtaac 12180
gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa 12240
ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct 12300
gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg 12360
cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc 12420
tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga 12480
agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa 12540
ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc 12600
gttatttctt gatgtctctg accagacacc catcaacagt attattttct cccatgaaga 12660
cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt 12720
agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct 12780
cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg 12840
ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc 12900
caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg 12960
tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc 13020
gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct 13080
gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa 13140
aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 13200
attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 13260
ttaatgtgag ttagcgcgaa ttgatctg 13288
<210> 84
<211> 11378
<212> DNA
<213> Artificial Sequence
<220>
<223> pT-HBSRYbbO
<400> 84
gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc 60
ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc 120
gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc 180
tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga 240
taacaatttc acacaggaaa cagaccatgg aattctacgt actagtgaat tcaggaggta 300
ataaatatgg gtctgatgct gattgattgg tgtgcactgg ctctggttgt tttcattggc 360
ctgccgcacg gcgcgctgga tgctgccatt tctttttcta tgatctcttc tgcaaaacgc 420
attgctcgtc tggctggtat tctgctgatc tatctgctgc tggcgaccgc gttcttcctg 480
atctggtatc agctgccagc gtttagcctg ctgatcttcc tgctgatctc cattatccac 540
tttggtatgg cagacttcaa cgcgtcccca agcaaactga aatggccgca tatcatcgcc 600
cacggcggtg ttgttactgt ttggctgccg ctgatccaga aaaacgaagt aactaaactg 660
tttagcatcc tgactaacgg tccgactccg atcctgtggg acatcctgct gattttcttc 720
ctgtgttggt ctattggcgt gtgtctgcac acgtacgaaa ccctgcgctc taaacattac 780
aacatcgcct ttgaactgat cggtctgatt ttcctggcgt ggtatgcgcc gcctctggtt 840
acgtttgcca cttacttctg cttcattcat tcccgtcgcc acttctcctt tgtgtggaag 900
cagctgcaac acatgtcttc caaaaagatg atgattggca gcgcgattat cctgtcctgt 960
acctcttggc tgatcggcgg tggtatctat ttcttcctga actccaaaat gatcgcctct 1020
gaggctgcgc tgcagactgt gttcatcggt ctggcggcac tgaccgtgcc gcacatgatt 1080
ctgatcgact tcatcttccg tccgcactct tcccgtatca aaatcaaaaa ctaattaatt 1140
aaaggaggta ataatatgac tcataaagca acggagatcc tgacaggtaa agttatgcaa 1200
aaatcggtct taattaccgg atgttccagt ggaattggcc tggaaagcgc gctcgaatta 1260
aaacgccagg gttttcatgt gctggcaggt tgccggaaac cggatgatgt tgagcgcatg 1320
aacagcatgg gatttaccgg cgtgttgatc gatctggatt caccagaaag tgttgatcgc 1380
gcagccgacg aggtgatcgc cctgaccgat aattgtctgt atgggatctt taacaatgcc 1440
ggattcggca tgtatggccc cctttccacc atcagccgtg cgcagatgga acagcagttt 1500
tccgccaact ttttcggcgc acaccagctc accatgcgcc tgttacccgc gatgttaccg 1560
cacggtgaag ggcgtattgt gatgacatca tcggtgatgg gattaatctc cacgccgggt 1620
cgtggcgctt acgcggccag taaatatgcg ctggaggcgt ggtcagatgc actgcgcatg 1680
gagctgcgcc acagcggaat taaagtcagc ctgatcgaac ccggtcccat tcgtactcgc 1740
ttcaccgaca acgtcaacca gacgcaaagt gataaaccag tcgaaaatcc cggcatcgcc 1800
gcccgcttta cgttgggacc ggaagcggtg gtggacaaag tacgccatgc ttttattagc 1860
gagaagccga agatgcgcta tccggtgacg ctggtgacct gggcggtaat ggtgcttaag 1920
cgcctgctgc cggggcgcgt gatggacaaa atattgcagg ggtaactagt aggaggtaat 1980
aaatatggtg agtggcagta aagcgggcgt ttcgcctcat cgcgaaatag aagtaatgag 2040
acaatccatt gacgatcacc tggctggcct gttacctgaa accgacagcc aggatatcgt 2100
cagccttgcg atgcgtgaag gcgtcatggc acccggtaaa cggatccgtc cgctgctgat 2160
gctgctggcc gcccgcgacc tccgctacca gggcagtatg cctacgctgc tcgatctcgc 2220
ctgcgccgtt gaactgaccc ataccgcgtc gctgatgctc gacgacatgc cctgcatgga 2280
caacgccgag ctgcgccgcg gtcagcccac tacccacaaa aaatttggtg agagcgtggc 2340
gatccttgcc tccgttgggc tgctctctaa agcctttggt ctgatcgccg ccaccggcga 2400
tctgccgggg gagaggcgtg cccaggcggt caacgagctc tctaccgccg tgggcgtgca 2460
gggcctggta ctggggcagt ttcgcgatct taacgatgcc gccctcgacc gtacccctga 2520
cgctatcctc agcaccaacc acctcaagac cggcattctg ttcagcgcga tgctgcagat 2580
cgtcgccatt gcttccgcct cgtcgccgag cacgcgagag acgctgcacg ccttcgccct 2640
cgacttcggc caggcgtttc aactgctgga cgatctgcgt gacgatcacc cggaaaccgg 2700
taaagatcgc aataaggacg cgggaaaatc gacgctggtc aaccggctgg gcgcagacgc 2760
ggcccggcaa aagctgcgcg agcatattga ttccgccgac aaacacctca cttttgcctg 2820
tccgcagggc ggcgccatcc gacagtttat gcatctgtgg tttggccatc accttgccga 2880
ctggtcaccg gtcatgaaaa tcgcctgata actcgaggag gtataaagga tgaaaaaaac 2940
cgttgtgatt ggcgcaggct ttggtggcct ggcgctggcg attcgcctgc aggcggcagg 3000
gatcccaacc gtactgctgg agcagcggga caagcccggc ggtcgggcct acgtctggca 3060
tgaccagggc tttacctttg acgccgggcc gacggtgatc accgatccta ccgcgcttga 3120
ggcgctgttc accctggccg gcaggcgcat ggaggattac gtcaggctgc tgccggtaaa 3180
acccttctac cgactctgct gggagtccgg gaagaccctc gactatgcta acgacagcgc 3240
cgagcttgag gcgcagatta cccagttcaa cccccgcgac gtcgagggct accggcgctt 3300
tctggcttac tcccaggcgg tattccagga gggatatttg cgcctcggca gcgtgccgtt 3360
cctctctttt cgcgacatgc tgcgcgccgg gccgcagctg cttaagctcc aggcgtggca 3420
gagcgtctac cagtcggttt cgcgctttat tgaggatgag catctgcggc aggccttctc 3480
gttccactcc ctgctggtag gcggcaaccc cttcaccacc tcgtccatct acaccctgat 3540
ccacgccctt gagcgggagt ggggggtctg gttccctgag ggcggcaccg gggcgctggt 3600
gaacggcatg gtgaagctgt ttaccgatct gggcggggag atcgaactca acgcccgggt 3660
cgaagagctg gtggtggccg ataaccgcgt aagccaggtc cggctggcgg atggtcggat 3720
ctttgacacc gacgccgtag cctcgaacgc tgacgtggtg aacacctata aaaagctgct 3780
cggccaccat ccggtggggc agaagcgggc ggcagcgctg gagcgcaaga gcatgagcaa 3840
ctcgctgttt gtgctctact tcggcctgaa ccagcctcat tcccagctgg cgcaccatac 3900
catctgtttt ggtccccgct accgggagct gatcgacgag atctttaccg gcagcgcgct 3960
ggcggatgac ttctcgctct acctgcactc gccctgcgtg accgatccct cgctcgcgcc 4020
tcccggctgc gccagcttct acgtgctggc cccggtgccg catcttggca acgcgccgct 4080
ggactgggcg caggaggggc cgaagctgcg cgaccgcatc tttgactacc ttgaagagcg 4140
ctatatgccc ggcctgcgta gccagctggt gacccagcgg atctttaccc cggcagactt 4200
ccacgacacg ctggatgcgc atctgggatc ggccttctcc atcgagccgc tgctgaccca 4260
aagcgcctgg ttccgcccgc acaaccgcga cagcgacatt gccaacctct acctggtggg 4320
cgcaggtact caccctgggg cgggcattcc tggcgtagtg gcctcggcga aagccaccgc 4380
cagcctgatg attgaggatc tgcaatgagc caaccgccgc tgcttgacca cgccacgcag 4440
accatggcca acggctcgaa aagttttgcc accgctgcga agctgttcga cccggccacc 4500
cgccgtagcg tgctgatgct ctacacctgg tgccgccact gcgatgacgt cattgacgac 4560
cagacccacg gcttcgccag cgaggccgcg gcggaggagg aggccaccca gcgcctggcc 4620
cggctgcgca cgctgaccct ggcggcgttt gaaggggccg agatgcagga tccggccttc 4680
gctgcctttc aggaggtggc gctgacccac ggtattacgc cccgcatggc gctcgatcac 4740
ctcgacggct ttgcgatgga cgtggctcag acccgctatg tcacctttga ggatacgctg 4800
cgctactgct atcacgtggc gggcgtggtg ggtctgatga tggccagggt gatgggcgtg 4860
cgggatgagc gggtgctgga tcgcgcctgc gatctggggc tggccttcca gctgacgaat 4920
atcgcccggg atattattga cgatgcggct attgaccgct gctatctgcc cgccgagtgg 4980
ctgcaggatg ccgggctgac cccggagaac tatgccgcgc gggagaatcg ggccgcgctg 5040
gcgcgggtgg cggagcggct tattgatgcc gcagagccgt actacatctc ctcccaggcc 5100
gggctacacg atctgccgcc gcgctgcgcc tgggcgatcg ccaccgcccg cagcgtctac 5160
cgggagatcg gtattaaggt aaaagcggcg ggaggcagcg cctgggatcg ccgccagcac 5220
accagcaaag gtgaaaaaat tgccatgctg atggcggcac cggggcaggt tattcgggcg 5280
aagacgacga gggtgacgcc gcgtccggcc ggtctttggc agcgtcccgt ttagtaatct 5340
agaggaggta ataaaatatg cttcgttcgt tgctcagagg cctcacgcat atcccccgcg 5400
tgaactccgc ccagcagccc agctgtgcac acgcgcgact ccagtttaag ctcaggagca 5460
tgcagatgac gctcatgcag cccagcatct cagccaatct gtcgcgcgcc gaggaccgca 5520
cagaccacat gaggggtgca agcacctggg caggcgggca gtcgcaggat gagctgatgc 5580
tgaaggacga gtgcatcttg gtggatgttg aggacaacat cacaggccat gccagcaagc 5640
tggagtgtca caagttccta ccacatcagc ctgcaggcct gctgcaccgg gccttctctg 5700
tgttcctgtt tgacgatcag gggcgactgc tgctgcaaca gcgtgcacgc tcaaaaatca 5760
ccttcccaag tgtgtggacg aacacctgct gcagccaccc tttacatggg cagaccccag 5820
atgaggtgga ccaactaagc caggtggccg acggaacagt acctggcgca aaggctgctg 5880
ccatccgcaa gttggagcac gagctgggga taccagcgca ccagctgccg gcaagcgcgt 5940
ttcgcttcct cacgcgtttg cactactgtg ccgcggacgt gcagccagct gcgacacaat 6000
cagcgctctg gggcgagcac gaaatggact acatcttgtt catccgggcc aacgtcacct 6060
tggcgcccaa ccctgacgag gtggacgaag tcaggtacgt gacgcaagag gagctgcggc 6120
agatgatgca gccggacaac gggctgcaat ggtcgccgtg gtttcgcatc atcgccgcgc 6180
gcttccttga gcgttggtgg gctgacctgg acgcggccct aaacactgac aaacacgagg 6240
attggggaac ggtgcatcac atcaacgaag cgtgataagc ggccgcgtcg acaggaggag 6300
cggctatgca accgcattat gatctgattc tcgtgggggc tggactcgcg aatggcctta 6360
tcgccctgcg tcttcagcag cagcaacctg atatgcgtat tttgcttatc gacgccgcac 6420
cccaggcggg cgggaatcat acgtggtcat ttcaccacga tgatttgact gagagccaac 6480
atcgttggat agctccgctg gtggttcatc actggcccga ctatcaggta cgctttccca 6540
cacgccgtcg taagctgaac agcggctact tttgtattac ttctcagcgt ttcgctgagg 6600
ttttacagcg acagtttggc ccgcacttgt ggatggatac cgcggtcgca gaggttaatg 6660
cggaatctgt tcggttgaaa aagggtcagg ttatcggtgc ccgcgcggtg attgacgggc 6720
ggggttatgc ggcaaattca gcactgagcg tgggcttcca ggcgtttatt ggccaggaat 6780
ggcgattgag ccacccgcat ggtttatcgt ctcccattat catggatgcc acggtcgatc 6840
agcaaaatgg ttatcgcttc gtgtacagcc tgccgctctc gccgaccaga ttgttaattg 6900
aagacacgca ctatattgat aatgcgacat tagatcctga atgcgcgcgg caaaatattt 6960
gcgactatgc cgcgcaacag ggttggcagc ttcagacact gctgcgagaa gaacagggcg 7020
ccttacccat tactctgtcg ggcaatgccg acgcattctg gcagcagcgc cccctggcct 7080
gtagtggatt acgtgccggt ctgttccatc ctaccaccgg ctattcactg ccgctggcgg 7140
ttgccgtggc cgaccgcctg agtgcacttg atgtctttac gtcggcctca attcaccatg 7200
ccattacgca ttttgcccgc gagcgctggc agcagcaggg ctttttccgc atgctgaatc 7260
gcatgctgtt tttagccgga cccgccgatt cacgctggcg ggttatgcag cgtttttatg 7320
gtttacctga agatttaatt gcccgttttt atgcgggaaa actcacgctg accgatcggc 7380
tacgtattct gagcggcaag ccgcctgttc cggtattagc agcattgcaa gccattatga 7440
cgactcatcg ttaactgcag gcatgcaagc tggggatcct acctgacgct ttttatcgca 7500
actctctact gtttctccat acccgttttt ttgggctggc ggatgagaga agattttcag 7560
cctgatacag attaaatcag aacgcagaag cggtctgata aaacagaatt tgcctggcgg 7620
cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc 7680
cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat caaataaaac 7740
gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc 7800
tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag 7860
ggtggcgggc aggacgcccg ccataaactg ccaggcatca aattaagcag aaggccatcc 7920
tgacggatgg cctttttgcg tttctacaaa ctctttttgt ttatttttct aaatacattc 7980
aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag 8040
gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg 8100
ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt 8160
gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt 8220
tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt 8280
attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa 8340
tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag 8400
agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac 8460
aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac 8520
tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac 8580
cacgatgcct acagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac 8640
tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact 8700
tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg 8760
tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt 8820
tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat 8880
aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta 8940
gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa 9000
tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 9060
aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 9120
aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 9180
tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc 9240
gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 9300
cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 9360
acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 9420
cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 9480
cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 9540
aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 9600
gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 9660
atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc 9720
tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga 9780
gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga 9840
agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg 9900
catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact 9960
ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac 10020
gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc 10080
gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagcagatc 10140
aattcgcgcg cgaaggcgaa gcggcatgca tttacgttga caccatcgaa tggtgcaaaa 10200
cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatgtga 10260
aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc 10320
gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga 10380
tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt 10440
tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg 10500
cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa 10560
gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc 10620
tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta 10680
atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt attattttct 10740
cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa 10800
tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc 10860
ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg 10920
ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga 10980
tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc 11040
tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt 11100
atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg 11160
accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct 11220
cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt 11280
tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag 11340
cgcaacgcaa ttaatgtgag ttagcgcgaa ttgatctg 11378
<210> 85
<211> 13938
<212> DNA
<213> Artificial Sequence
<220>
<223> pT-HBSREYbbO
<400> 85
tcgacaggag gagcggctat gcaaccgcat tatgatctga ttctcgtggg ggctggactc 60
gcgaatggcc ttatcgccct gcgtcttcag cagcagcaac ctgatatgcg tattttgctt 120
atcgacgccg caccccaggc gggcgggaat catacgtggt catttcacca cgatgatttg 180
actgagagcc aacatcgttg gatagctccg ctggtggttc atcactggcc cgactatcag 240
gtacgctttc ccacacgccg tcgtaagctg aacagcggct acttttgtat tacttctcag 300
cgtttcgctg aggttttaca gcgacagttt ggcccgcact tgtggatgga taccgcggtc 360
gcagaggtta atgcggaatc tgttcggttg aaaaagggtc aggttatcgg tgcccgcgcg 420
gtgattgacg ggcggggtta tgcggcaaat tcagcactga gcgtgggctt ccaggcgttt 480
attggccagg aatggcgatt gagccacccg catggtttat cgtctcccat tatcatggat 540
gccacggtcg atcagcaaaa tggttatcgc ttcgtgtaca gcctgccgct ctcgccgacc 600
agattgttaa ttgaagacac gcactatatt gataatgcga cattagatcc tgaatgcgcg 660
cggcaaaata tttgcgacta tgccgcgcaa cagggttggc agcttcagac actgctgcga 720
gaagaacagg gcgccttacc cattactctg tcgggcaatg ccgacgcatt ctggcagcag 780
cgccccctgg cctgtagtgg attacgtgcc ggtctgttcc atcctaccac cggctattca 840
ctgccgctgg cggttgccgt ggccgaccgc ctgagtgcac ttgatgtctt tacgtcggcc 900
tcaattcacc atgccattac gcattttgcc cgcgagcgct ggcagcagca gggctttttc 960
cgcatgctga atcgcatgct gtttttagcc ggacccgccg attcacgctg gcgggttatg 1020
cagcgttttt atggtttacc tgaagattta attgcccgtt tttatgcggg aaaactcacg 1080
ctgaccgatc ggctacgtat tctgagcggc aagccgcctg ttccggtatt agcagcattg 1140
caagccatta tgacgactca tcgttaactg caggcatgca agctggggat cctacctgac 1200
gctttttatc gcaactctct actgtttctc catacccgtt tttttgggct ggcggatgag 1260
agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga 1320
atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga 1380
aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg 1440
catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 1500
tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag 1560
caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaattaag 1620
cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactctttt tgtttatttt 1680
tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 1740
aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 1800
ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 1860
ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 1920
tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 1980
tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac 2040
actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 2100
gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 2160
acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 2220
gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 2280
acgagcgtga caccacgatg cctacagcaa tggcaacaac gttgcgcaaa ctattaactg 2340
gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 2400
ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 2460
gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 2520
cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 2580
agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 2640
catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 2700
tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 2760
cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 2820
gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 2880
taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc 2940
ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 3000
tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 3060
ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 3120
cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 3180
agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 3240
gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 3300
atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 3360
gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 3420
gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 3480
ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 3540
cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg 3600
gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa 3660
gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc 3720
aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc 3780
tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc 3840
gaggcagcag atcaattcgc gcgcgaaggc gaagcggcat gcatttacgt tgacaccatc 3900
gaatggtgca aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg 3960
gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat 4020
cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa 4080
gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg 4140
ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg 4200
caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg 4260
atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa 4320
cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa 4380
gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac 4440
agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg 4500
ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt 4560
ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa 4620
ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc 4680
gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt 4740
accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa 4800
gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg 4860
caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag 4920
ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc 4980
tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa 5040
agcgggcagt gagcgcaacg caattaatgt gagttagcgc gaattgatct ggtttgacag 5100
cttatcatcg actgcacggt gcaccaatgc ttctggcgtc aggcagccat cggaagctgt 5160
ggtatggctg tgcaggtcgt aaatcactgc ataattcgtg tcgctcaagg cgcactcccg 5220
ttctggataa tgttttttgc gccgacatca taacggttct ggcaaatatt ctgaaatgag 5280
ctgttgacaa ttaatcatcc ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt 5340
cacacaggaa acagaccatg gaattctacg tactagtgaa ttcaggaggt aataaatatg 5400
ggtctgatgc tgattgattg gtgtgcactg gctctggttg ttttcattgg cctgccgcac 5460
ggcgcgctgg atgctgccat ttctttttct atgatctctt ctgcaaaacg cattgctcgt 5520
ctggctggta ttctgctgat ctatctgctg ctggcgaccg cgttcttcct gatctggtat 5580
cagctgccag cgtttagcct gctgatcttc ctgctgatct ccattatcca ctttggtatg 5640
gcagacttca acgcgtcccc aagcaaactg aaatggccgc atatcatcgc ccacggcggt 5700
gttgttactg tttggctgcc gctgatccag aaaaacgaag taactaaact gtttagcatc 5760
ctgactaacg gtccgactcc gatcctgtgg gacatcctgc tgattttctt cctgtgttgg 5820
tctattggcg tgtgtctgca cacgtacgaa accctgcgct ctaaacatta caacatcgcc 5880
tttgaactga tcggtctgat tttcctggcg tggtatgcgc cgcctctggt tacgtttgcc 5940
acttacttct gcttcattca ttcccgtcgc cacttctcct ttgtgtggaa gcagctgcaa 6000
cacatgtctt ccaaaaagat gatgattggc agcgcgatta tcctgtcctg tacctcttgg 6060
ctgatcggcg gtggtatcta tttcttcctg aactccaaaa tgatcgcctc tgaggctgcg 6120
ctgcagactg tgttcatcgg tctggcggca ctgaccgtgc cgcacatgat tctgatcgac 6180
ttcatcttcc gtccgcactc ttcccgtatc aaaatcaaaa actaattaat taaaggaggt 6240
aataatatga ctcataaagc aacggagatc ctgacaggta aagttatgca aaaatcggtc 6300
ttaattaccg gatgttccag tggaattggc ctggaaagcg cgctcgaatt aaaacgccag 6360
ggttttcatg tgctggcagg ttgccggaaa ccggatgatg ttgagcgcat gaacagcatg 6420
ggatttaccg gcgtgttgat cgatctggat tcaccagaaa gtgttgatcg cgcagccgac 6480
gaggtgatcg ccctgaccga taattgtctg tatgggatct ttaacaatgc cggattcggc 6540
atgtatggcc ccctttccac catcagccgt gcgcagatgg aacagcagtt ttccgccaac 6600
tttttcggcg cacaccagct caccatgcgc ctgttacccg cgatgttacc gcacggtgaa 6660
gggcgtattg tgatgacatc atcggtgatg ggattaatct ccacgccggg tcgtggcgct 6720
tacgcggcca gtaaatatgc gctggaggcg tggtcagatg cactgcgcat ggagctgcgc 6780
cacagcggaa ttaaagtcag cctgatcgaa cccggtccca ttcgtactcg cttcaccgac 6840
aacgtcaacc agacgcaaag tgataaacca gtcgaaaatc ccggcatcgc cgcccgcttt 6900
acgttgggac cggaagcggt ggtggacaaa gtacgccatg cttttattag cgagaagccg 6960
aagatgcgct atccggtgac gctggtgacc tgggcggtaa tggtgcttaa gcgcctgctg 7020
ccggggcgcg tgatggacaa aatattgcag gggtaactag taggaggtaa taaatatggt 7080
gagtggcagt aaagcgggcg tttcgcctca tcgcgaaata gaagtaatga gacaatccat 7140
tgacgatcac ctggctggcc tgttacctga aaccgacagc caggatatcg tcagccttgc 7200
gatgcgtgaa ggcgtcatgg cacccggtaa acggatccgt ccgctgctga tgctgctggc 7260
cgcccgcgac ctccgctacc agggcagtat gcctacgctg ctcgatctcg cctgcgccgt 7320
tgaactgacc cataccgcgt cgctgatgct cgacgacatg ccctgcatgg acaacgccga 7380
gctgcgccgc ggtcagccca ctacccacaa aaaatttggt gagagcgtgg cgatccttgc 7440
ctccgttggg ctgctctcta aagcctttgg tctgatcgcc gccaccggcg atctgccggg 7500
ggagaggcgt gcccaggcgg tcaacgagct ctctaccgcc gtgggcgtgc agggcctggt 7560
actggggcag tttcgcgatc ttaacgatgc cgccctcgac cgtacccctg acgctatcct 7620
cagcaccaac cacctcaaga ccggcattct gttcagcgcg atgctgcaga tcgtcgccat 7680
tgcttccgcc tcgtcgccga gcacgcgaga gacgctgcac gccttcgccc tcgacttcgg 7740
ccaggcgttt caactgctgg acgatctgcg tgacgatcac ccggaaaccg gtaaagatcg 7800
caataaggac gcgggaaaat cgacgctggt caaccggctg ggcgcagacg cggcccggca 7860
aaagctgcgc gagcatattg attccgccga caaacacctc acttttgcct gtccgcaggg 7920
cggcgccatc cgacagttta tgcatctgtg gtttggccat caccttgccg actggtcacc 7980
ggtcatgaaa atcgcctgat aactcgagga ggtataaagg atgaaaaaaa ccgttgtgat 8040
tggcgcaggc tttggtggcc tggcgctggc gattcgcctg caggcggcag ggatcccaac 8100
cgtactgctg gagcagcggg acaagcccgg cggtcgggcc tacgtctggc atgaccaggg 8160
ctttaccttt gacgccgggc cgacggtgat caccgatcct accgcgcttg aggcgctgtt 8220
caccctggcc ggcaggcgca tggaggatta cgtcaggctg ctgccggtaa aacccttcta 8280
ccgactctgc tgggagtccg ggaagaccct cgactatgct aacgacagcg ccgagcttga 8340
ggcgcagatt acccagttca acccccgcga cgtcgagggc taccggcgct ttctggctta 8400
ctcccaggcg gtattccagg agggatattt gcgcctcggc agcgtgccgt tcctctcttt 8460
tcgcgacatg ctgcgcgccg ggccgcagct gcttaagctc caggcgtggc agagcgtcta 8520
ccagtcggtt tcgcgcttta ttgaggatga gcatctgcgg caggccttct cgttccactc 8580
cctgctggta ggcggcaacc ccttcaccac ctcgtccatc tacaccctga tccacgccct 8640
tgagcgggag tggggggtct ggttccctga gggcggcacc ggggcgctgg tgaacggcat 8700
ggtgaagctg tttaccgatc tgggcgggga gatcgaactc aacgcccggg tcgaagagct 8760
ggtggtggcc gataaccgcg taagccaggt ccggctggcg gatggtcgga tctttgacac 8820
cgacgccgta gcctcgaacg ctgacgtggt gaacacctat aaaaagctgc tcggccacca 8880
tccggtgggg cagaagcggg cggcagcgct ggagcgcaag agcatgagca actcgctgtt 8940
tgtgctctac ttcggcctga accagcctca ttcccagctg gcgcaccata ccatctgttt 9000
tggtccccgc taccgggagc tgatcgacga gatctttacc ggcagcgcgc tggcggatga 9060
cttctcgctc tacctgcact cgccctgcgt gaccgatccc tcgctcgcgc ctcccggctg 9120
cgccagcttc tacgtgctgg ccccggtgcc gcatcttggc aacgcgccgc tggactgggc 9180
gcaggagggg ccgaagctgc gcgaccgcat ctttgactac cttgaagagc gctatatgcc 9240
cggcctgcgt agccagctgg tgacccagcg gatctttacc ccggcagact tccacgacac 9300
gctggatgcg catctgggat cggccttctc catcgagccg ctgctgaccc aaagcgcctg 9360
gttccgcccg cacaaccgcg acagcgacat tgccaacctc tacctggtgg gcgcaggtac 9420
tcaccctggg gcgggcattc ctggcgtagt ggcctcggcg aaagccaccg ccagcctgat 9480
gattgaggat ctgcaatgag ccaaccgccg ctgcttgacc acgccacgca gaccatggcc 9540
aacggctcga aaagttttgc caccgctgcg aagctgttcg acccggccac ccgccgtagc 9600
gtgctgatgc tctacacctg gtgccgccac tgcgatgacg tcattgacga ccagacccac 9660
ggcttcgcca gcgaggccgc ggcggaggag gaggccaccc agcgcctggc ccggctgcgc 9720
acgctgaccc tggcggcgtt tgaaggggcc gagatgcagg atccggcctt cgctgccttt 9780
caggaggtgg cgctgaccca cggtattacg ccccgcatgg cgctcgatca cctcgacggc 9840
tttgcgatgg acgtggctca gacccgctat gtcacctttg aggatacgct gcgctactgc 9900
tatcacgtgg cgggcgtggt gggtctgatg atggccaggg tgatgggcgt gcgggatgag 9960
cgggtgctgg atcgcgcctg cgatctgggg ctggccttcc agctgacgaa tatcgcccgg 10020
gatattattg acgatgcggc tattgaccgc tgctatctgc ccgccgagtg gctgcaggat 10080
gccgggctga ccccggagaa ctatgccgcg cgggagaatc gggccgcgct ggcgcgggtg 10140
gcggagcggc ttattgatgc cgcagagccg tactacatct cctcccaggc cgggctacac 10200
gatctgccgc cgcgctgcgc ctgggcgatc gccaccgccc gcagcgtcta ccgggagatc 10260
ggtattaagg taaaagcggc gggaggcagc gcctgggatc gccgccagca caccagcaaa 10320
ggtgaaaaaa ttgccatgct gatggcggca ccggggcagg ttattcgggc gaagacgacg 10380
agggtgacgc cgcgtccggc cggtctttgg cagcgtcccg tttagtaatc tagaggaggt 10440
aataaaatat gcttcgttcg ttgctcagag gcctcacgca tatcccccgc gtgaactccg 10500
cccagcagcc cagctgtgca cacgcgcgac tccagtttaa gctcaggagc atgcagatga 10560
cgctcatgca gcccagcatc tcagccaatc tgtcgcgcgc cgaggaccgc acagaccaca 10620
tgaggggtgc aagcacctgg gcaggcgggc agtcgcagga tgagctgatg ctgaaggacg 10680
agtgcatctt ggtggatgtt gaggacaaca tcacaggcca tgccagcaag ctggagtgtc 10740
acaagttcct accacatcag cctgcaggcc tgctgcaccg ggccttctct gtgttcctgt 10800
ttgacgatca ggggcgactg ctgctgcaac agcgtgcacg ctcaaaaatc accttcccaa 10860
gtgtgtggac gaacacctgc tgcagccacc ctttacatgg gcagacccca gatgaggtgg 10920
accaactaag ccaggtggcc gacggaacag tacctggcgc aaaggctgct gccatccgca 10980
agttggagca cgagctgggg ataccagcgc accagctgcc ggcaagcgcg tttcgcttcc 11040
tcacgcgttt gcactactgt gccgcggacg tgcagccagc tgcgacacaa tcagcgctct 11100
ggggcgagca cgaaatggac tacatcttgt tcatccgggc caacgtcacc ttggcgccca 11160
accctgacga ggtggacgaa gtcaggtacg tgacgcaaga ggagctgcgg cagatgatgc 11220
agccggacaa cgggctgcaa tggtcgccgt ggtttcgcat catcgccgcg cgcttccttg 11280
agcgttggtg ggctgacctg gacgcggccc taaacactga caaacacgag gattggggaa 11340
cggtgcatca catcaacgaa gcgtgataag cggccgcgct gttgacaatt aatcatccgg 11400
ctcgtataat gtgtggaatt gtgagcggat aacaatttca cacaggaaac agaccatgga 11460
gttgaaaaca gtagttatta ttgatgcatt acgaacacca attggaaaat ataaaggcag 11520
cttaagtcaa gtaagtgccg tagacttagg aacacatgtt acaacacaac ttttaaaaag 11580
acattccact atttctgaag aaattgatca agtaatcttt ggaaatgttt tacaagctgg 11640
aaatggccaa aatcccgcac gacaaatagc aataaacagc ggtttatctc atgaaattcc 11700
cgcaatgaca gttaatgagg tctgcggatc aggaatgaag gccgttattt tggcgaaaca 11760
attgattcaa ttaggagaag cggaagtttt aattgctggc gggattgaga atatgtccca 11820
agcacctaaa ttacaacgat ttaattacga aacagaaagc tatgatgcgc ctttttctag 11880
tatgatgtac gatgggttaa cggatgcctt tagtggtcaa gcaatgggct taactgctga 11940
aaatgtggcc gaaaagtatc atgtaactag agaagagcaa gatcaatttt ctgtacattc 12000
acaattaaaa gcagctcaag cacaagcaga agggatattc gctgacgaaa tagccccatt 12060
agaagtatca ggaacgcttg tggagaaaga tgaagggatt cgccctaatt cgagcgttga 12120
gaagctagga acgcttaaaa cagtttttaa agaagacggt actgtaacag cagggaatgc 12180
atcaaccatt aatgatgggg cttctgcttt gattattgct tcacaagaat atgccgaagc 12240
acacggtctt ccttatttag ctattattcg agacagtgtg gaagtcggta ttgatccagc 12300
ctatatggga atttcgccga ttaaagccat tcaaaaactg ttagcgcgca atcaacttac 12360
tacggaagaa attgatctgt atgaaatcaa cgaagcattt gcagcaactt caatcgtggt 12420
ccaaagagaa ctggctttac cagaggaaaa ggtcaacatt tatggtggcg gtatttcatt 12480
aggtcatgcg attggtgcca caggtgctcg tttattaacg agtttaagtt atcaattaaa 12540
tcaaaaagaa aagaaatatg gagtggcttc tttatgtatc ggcggtggct taggactcgc 12600
tatgctacta gagagacctc agcaaaaaaa aaacagccga ttttatcaaa tgagtcctga 12660
ggaacgcctg gcttctcttc ttaatgaagg ccagatttct gctgatacaa aaaaagaatt 12720
tgaaaatacg gctttatctt cgcagattgc caatcatatg attgaaaatc aaatcagtga 12780
aacagaagtg ccgatgggcg ttggcttaca tttaacagtg gacgaaactg attatttggt 12840
accaatggcg acagaagagc cctcagtgat tgcggctttg agtaatggtg caaaaatagc 12900
acaaggattt aaaacagtga atcaacaacg tttaatgcgt ggacaaatcg ttttttacga 12960
tgttgcagac gccgagtcat tgattgatga actacaagta agagaaacgg aaatttttca 13020
acaagcagag ttaagttatc catctatcgt taaacgcggc ggcggcttaa gagatttgca 13080
atatcgtgct tttgatgaat catttgtatc tgtcgacttt ttagtagatg ttaaggatgc 13140
aatgggggca aatatcgtta acgctatgtt ggaaggtgtg gccgagttgt tccgtgaatg 13200
gtttgcggag caaaagattt tattcagtat tttaagtaat tatgccacgg agtcggttgt 13260
tacgatgaaa acggctattc cagtttcacg tttaagtaag gggagcaatg gccgggaaat 13320
tgctgaaaaa attgttttag cttcacgcta tgcttcatta gatccttatc gggcagtcac 13380
gcataacaaa gggatcatga atggcattga agctgtcgtt ttagctacag gaaatgatac 13440
acgcgctgtt agcgcttctt gtcatgcttt tgcggtgaag gaaggtcgct accaaggttt 13500
gactagttgg acgctggatg gcgaacaact aattggtgaa atttcagttc cgcttgcgtt 13560
agccacggtt ggcggtgcca caaaagtctt acctaaatct caagcagctg ctgatttgtt 13620
agcagtgacg gatgcaaaag aactaagtcg agtagtagcg gctgttggtt tggcacaaaa 13680
tttagcggcg ttacgggcct tagtctctga aggaattcaa aaaggacaca tggctctaca 13740
agcacgttct ttagcgatga cggtcggagc tactggtaaa gaagttgagg cagtcgctca 13800
acaattaaaa cgtcaaaaaa cgatgaacca agaccgagcc ttggctattt taaatgattt 13860
aagaaaacaa taaaaaaaca gttcagcaga aattattctg ctgaactgtt ttttttcaca 13920
ttaggtagcc gtttcagc 13938
<210> 86
<211> 12898
<212> DNA
<213> Artificial Sequence
<220>
<223> pT-HBSREYbbOfree
<400> 86
agcttgcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 60
gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 120
atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 180
atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 240
ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 300
gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 360
cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 420
tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 480
cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 540
ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 600
gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 660
tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg 720
ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc 780
gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc 840
atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg 900
catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg 960
acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta 1020
cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc 1080
gaaacgcgcg aggcagcaga tcaattcgcg cgcgaaggcg aagcggcatg catttacgtt 1140
gacaccatcg aatggtgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt 1200
caattcaggg tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt 1260
gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg 1320
cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa 1380
caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac 1440
gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg 1500
gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt 1560
ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt 1620
gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca 1680
cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg 1740
gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg 1800
cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg 1860
gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat 1920
gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg 1980
cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac 2040
gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc 2100
ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag 2160
ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg 2220
caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc 2280
cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagcgcg aattgatctg 2340
gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc 2400
ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc 2460
gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc 2520
tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga 2580
taacaatttc acacaggaaa cagaccatgg aattctacgt actagtgaat tcaggaggta 2640
ataaatatgg gtctgatgct gattgattgg tgtgcactgg ctctggttgt tttcattggc 2700
ctgccgcacg gcgcgctgga tgctgccatt tctttttcta tgatctcttc tgcaaaacgc 2760
attgctcgtc tggctggtat tctgctgatc tatctgctgc tggcgaccgc gttcttcctg 2820
atctggtatc agctgccagc gtttagcctg ctgatcttcc tgctgatctc cattatccac 2880
tttggtatgg cagacttcaa cgcgtcccca agcaaactga aatggccgca tatcatcgcc 2940
cacggcggtg ttgttactgt ttggctgccg ctgatccaga aaaacgaagt aactaaactg 3000
tttagcatcc tgactaacgg tccgactccg atcctgtggg acatcctgct gattttcttc 3060
ctgtgttggt ctattggcgt gtgtctgcac acgtacgaaa ccctgcgctc taaacattac 3120
aacatcgcct ttgaactgat cggtctgatt ttcctggcgt ggtatgcgcc gcctctggtt 3180
acgtttgcca cttacttctg cttcattcat tcccgtcgcc acttctcctt tgtgtggaag 3240
cagctgcaac acatgtcttc caaaaagatg atgattggca gcgcgattat cctgtcctgt 3300
acctcttggc tgatcggcgg tggtatctat ttcttcctga actccaaaat gatcgcctct 3360
gaggctgcgc tgcagactgt gttcatcggt ctggcggcac tgaccgtgcc gcacatgatt 3420
ctgatcgact tcatcttccg tccgcactct tcccgtatca aaatcaaaaa ctaattaatt 3480
aaaggaggta ataatatgac tcataaagca acggagatcc tgacaggtaa agttatgcaa 3540
aaatcggtct taattaccgg atgttccagt ggaattggcc tggaaagcgc gctcgaatta 3600
aaacgccagg gttttcatgt gctggcaggt tgccggaaac cggatgatgt tgagcgcatg 3660
aacagcatgg gatttaccgg cgtgttgatc gatctggatt caccagaaag tgttgatcgc 3720
gcagccgacg aggtgatcgc cctgaccgat aattgtctgt atgggatctt taacaatgcc 3780
ggattcggca tgtatggccc cctttccacc atcagccgtg cgcagatgga acagcagttt 3840
tccgccaact ttttcggcgc acaccagctc accatgcgcc tgttacccgc gatgttaccg 3900
cacggtgaag ggcgtattgt gatgacatca tcggtgatgg gattaatctc cacgccgggt 3960
cgtggcgctt acgcggccag taaatatgcg ctggaggcgt ggtcagatgc actgcgcatg 4020
gagctgcgcc acagcggaat taaagtcagc ctgatcgaac ccggtcccat tcgtactcgc 4080
ttcaccgaca acgtcaacca gacgcaaagt gataaaccag tcgaaaatcc cggcatcgcc 4140
gcccgcttta cgttgggacc ggaagcggtg gtggacaaag tacgccatgc ttttattagc 4200
gagaagccga agatgcgcta tccggtgacg ctggtgacct gggcggtaat ggtgcttaag 4260
cgcctgctgc cggggcgcgt gatggacaaa atattgcagg ggtaactagt aggaggtaat 4320
aaatatggtg agtggcagta aagcgggcgt ttcgcctcat cgcgaaatag aagtaatgag 4380
acaatccatt gacgatcacc tggctggcct gttacctgaa accgacagcc aggatatcgt 4440
cagccttgcg atgcgtgaag gcgtcatggc acccggtaaa cggatccgtc cgctgctgat 4500
gctgctggcc gcccgcgacc tccgctacca gggcagtatg cctacgctgc tcgatctcgc 4560
ctgcgccgtt gaactgaccc ataccgcgtc gctgatgctc gacgacatgc cctgcatgga 4620
caacgccgag ctgcgccgcg gtcagcccac tacccacaaa aaatttggtg agagcgtggc 4680
gatccttgcc tccgttgggc tgctctctaa agcctttggt ctgatcgccg ccaccggcga 4740
tctgccgggg gagaggcgtg cccaggcggt caacgagctc tctaccgccg tgggcgtgca 4800
gggcctggta ctggggcagt ttcgcgatct taacgatgcc gccctcgacc gtacccctga 4860
cgctatcctc agcaccaacc acctcaagac cggcattctg ttcagcgcga tgctgcagat 4920
cgtcgccatt gcttccgcct cgtcgccgag cacgcgagag acgctgcacg ccttcgccct 4980
cgacttcggc caggcgtttc aactgctgga cgatctgcgt gacgatcacc cggaaaccgg 5040
taaagatcgc aataaggacg cgggaaaatc gacgctggtc aaccggctgg gcgcagacgc 5100
ggcccggcaa aagctgcgcg agcatattga ttccgccgac aaacacctca cttttgcctg 5160
tccgcagggc ggcgccatcc gacagtttat gcatctgtgg tttggccatc accttgccga 5220
ctggtcaccg gtcatgaaaa tcgcctgata actcgaggag gtataaagga tgaaaaaaac 5280
cgttgtgatt ggcgcaggct ttggtggcct ggcgctggcg attcgcctgc aggcggcagg 5340
gatcccaacc gtactgctgg agcagcggga caagcccggc ggtcgggcct acgtctggca 5400
tgaccagggc tttacctttg acgccgggcc gacggtgatc accgatccta ccgcgcttga 5460
ggcgctgttc accctggccg gcaggcgcat ggaggattac gtcaggctgc tgccggtaaa 5520
acccttctac cgactctgct gggagtccgg gaagaccctc gactatgcta acgacagcgc 5580
cgagcttgag gcgcagatta cccagttcaa cccccgcgac gtcgagggct accggcgctt 5640
tctggcttac tcccaggcgg tattccagga gggatatttg cgcctcggca gcgtgccgtt 5700
cctctctttt cgcgacatgc tgcgcgccgg gccgcagctg cttaagctcc aggcgtggca 5760
gagcgtctac cagtcggttt cgcgctttat tgaggatgag catctgcggc aggccttctc 5820
gttccactcc ctgctggtag gcggcaaccc cttcaccacc tcgtccatct acaccctgat 5880
ccacgccctt gagcgggagt ggggggtctg gttccctgag ggcggcaccg gggcgctggt 5940
gaacggcatg gtgaagctgt ttaccgatct gggcggggag atcgaactca acgcccgggt 6000
cgaagagctg gtggtggccg ataaccgcgt aagccaggtc cggctggcgg atggtcggat 6060
ctttgacacc gacgccgtag cctcgaacgc tgacgtggtg aacacctata aaaagctgct 6120
cggccaccat ccggtggggc agaagcgggc ggcagcgctg gagcgcaaga gcatgagcaa 6180
ctcgctgttt gtgctctact tcggcctgaa ccagcctcat tcccagctgg cgcaccatac 6240
catctgtttt ggtccccgct accgggagct gatcgacgag atctttaccg gcagcgcgct 6300
ggcggatgac ttctcgctct acctgcactc gccctgcgtg accgatccct cgctcgcgcc 6360
tcccggctgc gccagcttct acgtgctggc cccggtgccg catcttggca acgcgccgct 6420
ggactgggcg caggaggggc cgaagctgcg cgaccgcatc tttgactacc ttgaagagcg 6480
ctatatgccc ggcctgcgta gccagctggt gacccagcgg atctttaccc cggcagactt 6540
ccacgacacg ctggatgcgc atctgggatc ggccttctcc atcgagccgc tgctgaccca 6600
aagcgcctgg ttccgcccgc acaaccgcga cagcgacatt gccaacctct acctggtggg 6660
cgcaggtact caccctgggg cgggcattcc tggcgtagtg gcctcggcga aagccaccgc 6720
cagcctgatg attgaggatc tgcaatgagc caaccgccgc tgcttgacca cgccacgcag 6780
accatggcca acggctcgaa aagttttgcc accgctgcga agctgttcga cccggccacc 6840
cgccgtagcg tgctgatgct ctacacctgg tgccgccact gcgatgacgt cattgacgac 6900
cagacccacg gcttcgccag cgaggccgcg gcggaggagg aggccaccca gcgcctggcc 6960
cggctgcgca cgctgaccct ggcggcgttt gaaggggccg agatgcagga tccggccttc 7020
gctgcctttc aggaggtggc gctgacccac ggtattacgc cccgcatggc gctcgatcac 7080
ctcgacggct ttgcgatgga cgtggctcag acccgctatg tcacctttga ggatacgctg 7140
cgctactgct atcacgtggc gggcgtggtg ggtctgatga tggccagggt gatgggcgtg 7200
cgggatgagc gggtgctgga tcgcgcctgc gatctggggc tggccttcca gctgacgaat 7260
atcgcccggg atattattga cgatgcggct attgaccgct gctatctgcc cgccgagtgg 7320
ctgcaggatg ccgggctgac cccggagaac tatgccgcgc gggagaatcg ggccgcgctg 7380
gcgcgggtgg cggagcggct tattgatgcc gcagagccgt actacatctc ctcccaggcc 7440
gggctacacg atctgccgcc gcgctgcgcc tgggcgatcg ccaccgcccg cagcgtctac 7500
cgggagatcg gtattaaggt aaaagcggcg ggaggcagcg cctgggatcg ccgccagcac 7560
accagcaaag gtgaaaaaat tgccatgctg atggcggcac cggggcaggt tattcgggcg 7620
aagacgacga gggtgacgcc gcgtccggcc ggtctttggc agcgtcccgt ttagtaatct 7680
agaggaggta ataaaatatg cttcgttcgt tgctcagagg cctcacgcat atcccccgcg 7740
tgaactccgc ccagcagccc agctgtgcac acgcgcgact ccagtttaag ctcaggagca 7800
tgcagatgac gctcatgcag cccagcatct cagccaatct gtcgcgcgcc gaggaccgca 7860
cagaccacat gaggggtgca agcacctggg caggcgggca gtcgcaggat gagctgatgc 7920
tgaaggacga gtgcatcttg gtggatgttg aggacaacat cacaggccat gccagcaagc 7980
tggagtgtca caagttccta ccacatcagc ctgcaggcct gctgcaccgg gccttctctg 8040
tgttcctgtt tgacgatcag gggcgactgc tgctgcaaca gcgtgcacgc tcaaaaatca 8100
ccttcccaag tgtgtggacg aacacctgct gcagccaccc tttacatggg cagaccccag 8160
atgaggtgga ccaactaagc caggtggccg acggaacagt acctggcgca aaggctgctg 8220
ccatccgcaa gttggagcac gagctgggga taccagcgca ccagctgccg gcaagcgcgt 8280
ttcgcttcct cacgcgtttg cactactgtg ccgcggacgt gcagccagct gcgacacaat 8340
cagcgctctg gggcgagcac gaaatggact acatcttgtt catccgggcc aacgtcacct 8400
tggcgcccaa ccctgacgag gtggacgaag tcaggtacgt gacgcaagag gagctgcggc 8460
agatgatgca gccggacaac gggctgcaat ggtcgccgtg gtttcgcatc atcgccgcgc 8520
gcttccttga gcgttggtgg gctgacctgg acgcggccct aaacactgac aaacacgagg 8580
attggggaac ggtgcatcac atcaacgaag cgtgataagc ggccgcgctg ttgacaatta 8640
atcatccggc tcgtataatg tgtggaattg tgagcggata acaatttcac acaggaaaca 8700
gaccatggag ttgaaaacag tagttattat tgatgcatta cgaacaccaa ttggaaaata 8760
taaaggcagc ttaagtcaag taagtgccgt agacttagga acacatgtta caacacaact 8820
tttaaaaaga cattccacta tttctgaaga aattgatcaa gtaatctttg gaaatgtttt 8880
acaagctgga aatggccaaa atcccgcacg acaaatagca ataaacagcg gtttatctca 8940
tgaaattccc gcaatgacag ttaatgaggt ctgcggatca ggaatgaagg ccgttatttt 9000
ggcgaaacaa ttgattcaat taggagaagc ggaagtttta attgctggcg ggattgagaa 9060
tatgtcccaa gcacctaaat tacaacgatt taattacgaa acagaaagct atgatgcgcc 9120
tttttctagt atgatgtacg atgggttaac ggatgccttt agtggtcaag caatgggctt 9180
aactgctgaa aatgtggccg aaaagtatca tgtaactaga gaagagcaag atcaattttc 9240
tgtacattca caattaaaag cagctcaagc acaagcagaa gggatattcg ctgacgaaat 9300
agccccatta gaagtatcag gaacgcttgt ggagaaagat gaagggattc gccctaattc 9360
gagcgttgag aagctaggaa cgcttaaaac agtttttaaa gaagacggta ctgtaacagc 9420
agggaatgca tcaaccatta atgatggggc ttctgctttg attattgctt cacaagaata 9480
tgccgaagca cacggtcttc cttatttagc tattattcga gacagtgtgg aagtcggtat 9540
tgatccagcc tatatgggaa tttcgccgat taaagccatt caaaaactgt tagcgcgcaa 9600
tcaacttact acggaagaaa ttgatctgta tgaaatcaac gaagcatttg cagcaacttc 9660
aatcgtggtc caaagagaac tggctttacc agaggaaaag gtcaacattt atggtggcgg 9720
tatttcatta ggtcatgcga ttggtgccac aggtgctcgt ttattaacga gtttaagtta 9780
tcaattaaat caaaaagaaa agaaatatgg agtggcttct ttatgtatcg gcggtggctt 9840
aggactcgct atgctactag agagacctca gcaaaaaaaa aacagccgat tttatcaaat 9900
gagtcctgag gaacgcctgg cttctcttct taatgaaggc cagatttctg ctgatacaaa 9960
aaaagaattt gaaaatacgg ctttatcttc gcagattgcc aatcatatga ttgaaaatca 10020
aatcagtgaa acagaagtgc cgatgggcgt tggcttacat ttaacagtgg acgaaactga 10080
ttatttggta ccaatggcga cagaagagcc ctcagtgatt gcggctttga gtaatggtgc 10140
aaaaatagca caaggattta aaacagtgaa tcaacaacgt ttaatgcgtg gacaaatcgt 10200
tttttacgat gttgcagacg ccgagtcatt gattgatgaa ctacaagtaa gagaaacgga 10260
aatttttcaa caagcagagt taagttatcc atctatcgtt aaacgcggcg gcggcttaag 10320
agatttgcaa tatcgtgctt ttgatgaatc atttgtatct gtcgactttt tagtagatgt 10380
taaggatgca atgggggcaa atatcgttaa cgctatgttg gaaggtgtgg ccgagttgtt 10440
ccgtgaatgg tttgcggagc aaaagatttt attcagtatt ttaagtaatt atgccacgga 10500
gtcggttgtt acgatgaaaa cggctattcc agtttcacgt ttaagtaagg ggagcaatgg 10560
ccgggaaatt gctgaaaaaa ttgttttagc ttcacgctat gcttcattag atccttatcg 10620
ggcagtcacg cataacaaag ggatcatgaa tggcattgaa gctgtcgttt tagctacagg 10680
aaatgataca cgcgctgtta gcgcttcttg tcatgctttt gcggtgaagg aaggtcgcta 10740
ccaaggtttg actagttgga cgctggatgg cgaacaacta attggtgaaa tttcagttcc 10800
gcttgcgtta gccacggttg gcggtgccac aaaagtctta cctaaatctc aagcagctgc 10860
tgatttgtta gcagtgacgg atgcaaaaga actaagtcga gtagtagcgg ctgttggttt 10920
ggcacaaaat ttagcggcgt tacgggcctt agtctctgaa ggaattcaaa aaggacacat 10980
ggctctacaa gcacgttctt tagcgatgac ggtcggagct actggtaaag aagttgaggc 11040
agtcgctcaa caattaaaac gtcaaaaaac gatgaaccaa gaccgagcct tggctatttt 11100
aaatgattta agaaaacaat aaaaaaacag ttcagcagaa attattctgc tgaactgttt 11160
tttttcacat taggtagccg tttcagctcg acaggaggag cggctatgca accgcattat 11220
gatctgattc tcgtgggggc tggactcgcg aatggcctta tcgccctgcg tcttcagcag 11280
cagcaacctg atatgcgtat tttgcttatc gacgccgcac cccaggcggg cgggaatcat 11340
acgtggtcat ttcaccacga tgatttgact gagagccaac atcgttggat agctccgctg 11400
gtggttcatc actggcccga ctatcaggta cgctttccca cacgccgtcg taagctgaac 11460
agcggctact tttgtattac ttctcagcgt ttcgctgagg ttttacagcg acagtttggc 11520
ccgcacttgt ggatggatac cgcggtcgca gaggttaatg cggaatctgt tcggttgaaa 11580
aagggtcagg ttatcggtgc ccgcgcggtg attgacgggc ggggttatgc ggcaaattca 11640
gcactgagcg tgggcttcca ggcgtttatt ggccaggaat ggcgattgag ccacccgcat 11700
ggtttatcgt ctcccattat catggatgcc acggtcgatc agcaaaatgg ttatcgcttc 11760
gtgtacagcc tgccgctctc gccgaccaga ttgttaattg aagacacgca ctatattgat 11820
aatgcgacat tagatcctga atgcgcgcgg caaaatattt gcgactatgc cgcgcaacag 11880
ggttggcagc ttcagacact gctgcgagaa gaacagggcg ccttacccat tactctgtcg 11940
ggcaatgccg acgcattctg gcagcagcgc cccctggcct gtagtggatt acgtgccggt 12000
ctgttccatc ctaccaccgg ctattcactg ccgctggcgg ttgccgtggc cgaccgcctg 12060
agtgcacttg atgtctttac gtcggcctca attcaccatg ccattacgca ttttgcccgc 12120
gagcgctggc agcagcaggg ctttttccgc atgctgaatc gcatgctgtt tttagccgga 12180
cccgccgatt cacgctggcg ggttatgcag cgtttttatg gtttacctga agatttaatt 12240
gcccgttttt atgcgggaaa actcacgctg accgatcggc tacgtattct gagcggcaag 12300
ccgcctgttc cggtattagc agcattgcaa gccattatga cgactcatcg ttaactgcag 12360
gcatgcaagc tggggatcct acctgacgct ttttatcgca actctctact gtttctccat 12420
acccgttttt ttgggctggc ggatgagaga agattttcag cctgatacag attaaatcag 12480
aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac 12540
ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc 12600
cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac 12660
tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg 12720
ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg 12780
ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg 12840
tttctacaaa ctctttttgt ttatttttct aaatacattc aaatatgtat ccgctcaa 12898
<210> 87
<211> 5381
<212> DNA
<213> Artificial Sequence
<220>
<223> pT-dxr
<400> 87
aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg 60
atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg 120
atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa 180
ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac 240
tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa 300
aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 360
tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat 420
gtgagttagc gcgaattgat ctggtttgac agcttatcat cgactgcacg gtgcaccaat 480
gcttctggcg tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact 540
gcataattcg tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat 600
cataacggtt ctggcaaata ttctgaaatg agctgttgac aattaatcat ccggctcgta 660
taatgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagacca tggaattcga 720
gctcatgaag caactcacca ttctgggctc gaccggctcg attggttgca gcacgctgga 780
cgtggtgcgc cataatcccg aacacttccg cgtagttgcg ctggtggcag gcaaaaatgt 840
cactcgcatg gtagaacagt gcctggaatt ctctccccgc tatgccgtaa tggacgatga 900
agcgagtgcg aaacttctta aaacgatgct acagcaacag ggtagccgca ccgaagtctt 960
aagtgggcaa caagccgctt gcgatatggc agcgcttgag gatgttgatc aggtgatggc 1020
agccattgtt ggcgctgctg ggctgttacc tacgcttgct gcgatccgcg cgggtaaaac 1080
cattttgctg gccaataaag aatcactggt tacctgcgga cgtctgttta tggacgccgt 1140
aaagcagagc aaagcgcaat tgttaccggt cgatagcgaa cataacgcca tttttcagag 1200
tttaccgcaa cctatccagc ataatctggg atacgctgac cttgagcaaa atggcgtggt 1260
gtccatttta cttaccgggt ctggtggccc tttccgtgag acgccattgc gcgatttggc 1320
aacaatgacg ccggatcaag cctgccgtca tccgaactgg tcgatggggc gtaaaatttc 1380
tgtcgattcg gctaccatga tgaacaaagg tctggaatac attgaagcgc gttggctgtt 1440
taacgccagc gccagccaga tggaagtgct gattcacccg cagtcagtga ttcactcaat 1500
ggtgcgctat caggacggca gtgttctggc gcagctgggg gaaccggata tgcgtacgcc 1560
aattgcccac accatggcat ggccgaatcg cgtgaactct ggcgtgaagc cgctcgattt 1620
ttgcaaacta agtgcgttga catttgccgc accggattat gatcgttatc catgcctgaa 1680
actggcgatg gaggcgttcg aacaaggcca ggcagcgacg acagcattga atgccgcaaa 1740
cgaaatcacc gttgctgctt ttcttgcgca acaaatccgc tttacggata tcgctgcgtt 1800
gaatttatcc gtactggaaa aaatggatat gcgcgaacca caatgtgtgg acgatgtgtt 1860
atctgttgat gcgaacgcgc gtgaagtcgc cagaaaagag gtgatgcgtc tcgcaagctg 1920
atctagagcg ggggatccac tagttctaga gtcgacctgc aggcatgcaa gcttggctgt 1980
tttggcggat gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt 2040
ctgataaaac agaatttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg 2100
aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta 2160
gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 2220
tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt 2280
gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag 2340
gcatcaaatt aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct 2400
ttttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga 2460
taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc 2520
cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg 2580
aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc 2640
aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact 2700
tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc 2760
ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag 2820
catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat 2880
aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt 2940
ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa 3000
gccataccaa acgacgagcg tgacaccacg atgcctacag caatggcaac aacgttgcgc 3060
aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg 3120
gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt 3180
gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca 3240
gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat 3300
gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca 3360
gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 3420
atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 3480
ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 3540
ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 3600
ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 3660
ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 3720
ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 3780
tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 3840
tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 3900
tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 3960
tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 4020
gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 4080
tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 4140
ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 4200
gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc 4260
gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt 4320
acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat 4380
gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc 4440
cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg 4500
cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat 4560
caccgaaacg cgcgaggcag cagatcaatt cgcgcgcgaa ggcgaagcgg catgcattta 4620
cgttgacacc atcgaatggt gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga 4680
gagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc 4740
cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa 4800
aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc 4860
acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct 4920
gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag 4980
cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa 5040
tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc 5100
cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca 5160
gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca 5220
tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc 5280
ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat 5340
agcggaacgg gaaggcgact ggagtgccat gtccggtttt c 5381
<210> 88
<211> 7999
<212> DNA
<213> Artificial Sequence
<220>
<223> pTAS-dxr
<400> 88
tcgacctgca ggcatgcaag cttggctgtt ttggcggatg agagaagatt ttcagcctga 60
tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct ggcggcagta 120
gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg 180
gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag 240
gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg 300
agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg 360
cgggcaggac gcccgccata aactgccagg catcaaatta agcagaaggc catcctgacg 420
gatggccttt ttgcgtttct acaaactctt tttgtttatt tttctaaata cattcaaata 480
tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga 540
gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc 600
ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg 660
cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc 720
ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat 780
cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact 840
tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat 900
tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga 960
tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc 1020
ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga 1080
tgcctacagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag 1140
cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc 1200
gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt 1260
ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct 1320
acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg 1380
cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg 1440
atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca 1500
tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga 1560
tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 1620
aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 1680
aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt 1740
taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 1800
taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 1860
agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 1920
tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 1980
cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 2040
agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 2100
gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 2160
aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 2220
tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag 2280
ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg 2340
aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat 2400
ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct 2460
atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc 2520
ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag 2580
ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc agatcaattc 2640
gcgcgcgaag gcgaagcggc atgcatttac gttgacacca tcgaatggtg caaaaccttt 2700
cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca 2760
gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg 2820
gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg 2880
gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg 2940
attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt 3000
aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc 3060
gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc 3120
attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt 3180
ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat 3240
gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg 3300
ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa 3360
tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg 3420
tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg 3480
gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc 3540
gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc 3600
ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc 3660
ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg 3720
gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc 3780
gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 3840
cgcaattaat gtgagttagc gcgaattgat ctggtttgac agcttatcat cgactgcacg 3900
gtgcaccaat gcttctggcg tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc 3960
gtaaatcact gcataattcg tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt 4020
gcgccgacat cataacggtt ctggcaaata ttctgaaatg agctgttgac aattaatcat 4080
ccggctcgta taatgtgtgg aattgtgagc ggataacaat ttcacacagg aggtaataaa 4140
tatggaattc atggactttc cgcagcaact cgaagcctgc gttaagcagg ccaaccaggc 4200
gctgagccgt tttatcgccc cactgccctt tcagaacact cccgtggtcg aaaccatgca 4260
gtatggcgca ttattaggtg gtaagcgcct gcgacctttc ctggtttatg ccaccggtca 4320
tatgttcggc gttagcacaa acacgctgga cgcacccgct gccgccgttg agtgtatcca 4380
cgcttactca ttaattcatg atgatttacc ggcaatggat gatgacgatc tgcgtcgcgg 4440
tttgccaacc tgccatgtga agtttggcga agcaaacgcg attctcgctg gcgacgcttt 4500
acaaacgctg gcgttctcga ttttaagcga tgccgatatg ccggaagtgt cggaccgcga 4560
cagaatttcg atgatttctg aactggcgag cgccagtggt attgccggaa tgtgcggtgg 4620
tcaggcatta gatttagacg cggaaggcaa acacgtacct ctggacgcgc ttgagcgtat 4680
tcatcgtcat aaaaccggcg cattgattcg cgccgccgtt cgccttggtg cattaagcgc 4740
cggagataaa ggacgtcgtg ctctgccggt actcgacaag tatgcagaga gcatcggcct 4800
tgccttccag gttcaggatg acatcctgga tgtggtggga gatactgcaa cgttgggaaa 4860
acgccagggt gccgaccagc aacttggtaa aagtacctac cctgcacttc tgggtcttga 4920
gcaagcccgg aagaaagccc gggatctgat cgacgatgcc cgtcagtcgc tgaaacaact 4980
ggctgaacag tcactcgata cctcggcact ggaagcgcta gcggactaca tcatccagcg 5040
taataaataa ggatccaaaa aggaggtaat aaaccatgtc aactcaacaa gtttcatcag 5100
agaacattgt tcgtaacgct gcgaatttcc atcctaatat atggggaaac catttcctca 5160
catgtccttc tcagacgatt gatagttgga ctcaacagca ccacaaagaa ctgaaagaag 5220
aggtgaggaa aatgatggtg tctgatgcaa ataaacctgc ccagagattg cgcttgattg 5280
atactgtcca aaggctaggt gtggcttacc actttgaaaa ggagattgat gatgcattgg 5340
agaaaatagg tcatgaccct tttgatgata aagatgatct ctacattgtc tctctttgtt 5400
ttcgattgct gaggcagcat ggaattaaga tatcatgtga tgtgtttgag aagtttaaag 5460
atgacgatgg aaaattcaag gcatcattga tgaatgatgt tcaaggcatg ctaagtttat 5520
atgaggcagc acacctagcc attcacggag aagatatttt agatgaagca attgttttca 5580
cgaccactca ccttaagtca acggtatcta attctcctgt aaactctact tttgctgaac 5640
aaatacgtca ttctctcaga gttcctctcc gtaaagctgt acctaggtta gagtcgaggt 5700
atttcttgga tatctattca agagatgatt tgcacgataa aactttgctc aatttcgcaa 5760
agttagactt taatatacta caagcaatgc accagaagga agcaagtgag atgaccaggt 5820
ggtggagaga ttttgacttc cttaaaaagc tgccttatat aagagacaga gtcgtggagc 5880
tatatttttg gattctggtg ggagtgtctt atcagcccaa attcagcact ggtagaattt 5940
ttttgtccaa aataatatgc cttgagaccc tcgtagatga tacatttgac gcctacggta 6000
cttttgacga gctcgcaatc tttactgaag cagttacaag atgggacctt ggccacagag 6060
atgcactacc agaatacatg aaattcattt tcaagacact cattgatgtc tacagtgaag 6120
ctgagcaaga actggcaaag gaagggagat catacagcat acactatgca atacgatcgt 6180
tccaagaact agttatgaag tacttctgcg aagccaagtg gttaaataaa ggttatgttc 6240
cgagcctgga cgattataaa tcagtttcat taagaagtat cggtttttta ccgatagcgg 6300
tagcttcctt cgttttcatg ggtgatattg caactaagga ggtctttgaa tgggaaatga 6360
ataaccctaa gatcataata gccgcagaaa cgattttcag attcctggat gacatagcag 6420
gccataggtt tgagcaaaag agagaacata gtccatcagc tattgaatgc tacaagaatc 6480
aacatggagt gtctgaggaa gaggcagtta aagcgttgtc gttagaagtt gctaatagtt 6540
ggaaagatat aaatgaggag ctgcttctca acccaatggc tattccttta cctctgcttc 6600
aggtgattct tgatctctca cgttcggccg attttatgta cggtaatgct caagatcgct 6660
tcacgcattc aacgatgatg aaagaccaag ttgatttggt gctgaaggac cccgttaagc 6720
ttgacgatta aagatctgtg tggaattgtg agcggataac aatttcacac aggaaacaga 6780
ccatggaatt cgagctcatg aagcaactca ccattctggg ctcgaccggc tcgattggtt 6840
gcagcacgct ggacgtggtg cgccataatc ccgaacactt ccgcgtagtt gcgctggtgg 6900
caggcaaaaa tgtcactcgc atggtagaac agtgcctgga attctctccc cgctatgccg 6960
taatggacga tgaagcgagt gcgaaacttc ttaaaacgat gctacagcaa cagggtagcc 7020
gcaccgaagt cttaagtggg caacaagccg cttgcgatat ggcagcgctt gaggatgttg 7080
atcaggtgat ggcagccatt gttggcgctg ctgggctgtt acctacgctt gctgcgatcc 7140
gcgcgggtaa aaccattttg ctggccaata aagaatcact ggttacctgc ggacgtctgt 7200
ttatggacgc cgtaaagcag agcaaagcgc aattgttacc ggtcgatagc gaacataacg 7260
ccatttttca gagtttaccg caacctatcc agcataatct gggatacgct gaccttgagc 7320
aaaatggcgt ggtgtccatt ttacttaccg ggtctggtgg ccctttccgt gagacgccat 7380
tgcgcgattt ggcaacaatg acgccggatc aagcctgccg tcatccgaac tggtcgatgg 7440
ggcgtaaaat ttctgtcgat tcggctacca tgatgaacaa aggtctggaa tacattgaag 7500
cgcgttggct gtttaacgcc agcgccagcc agatggaagt gctgattcac ccgcagtcag 7560
tgattcactc aatggtgcgc tatcaggacg gcagtgttct ggcgcagctg ggggaaccgg 7620
atatgcgtac gccaattgcc cacaccatgg catggccgaa tcgcgtgaac tctggcgtga 7680
agccgctcga tttttgcaaa ctaagtgcgt tgacatttgc cgcaccggat tatgatcgtt 7740
atccatgcct gaaactggcg atggaggcgt tcgaacaagg ccaggcagcg acgacagcat 7800
tgaatgccgc aaacgaaatc accgttgctg cttttcttgc gcaacaaatc cgctttacgg 7860
atatcgctgc gttgaattta tccgtactgg aaaaaatgga tatgcgcgaa ccacaatgtg 7920
tggacgatgt gttatctgtt gatgcgaacg cgcgtgaagt cgccagaaaa gaggtgatgc 7980
gtctcgcaag ctgatctac 7999
<210> 89
<211> 7275
<212> DNA
<213> Artificial Sequence
<220>
<223> pT-dxr/s
<400> 89
gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc 60
ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc 120
gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc 180
tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga 240
taacaatttc acacaggaaa cagaccatgg aattcatgag ttttgatatt gccaaatacc 300
cgaccctggc actggtcgac tccacccagg agttacgact gttgccgaaa gagagtttac 360
cgaaactctg cgacgaactg cgccgctatt tactcgacag cgtgagccgt tccagcgggc 420
acttcgcctc cgggctgggc acggtcgaac tgaccgtggc gctgcactat gtctacaaca 480
ccccgtttga ccaattgatt tgggatgtgg ggcatcaggc ttatccgcat aaaattttga 540
ccggacgccg cgacaaaatc ggcaccatcc gtcagaaagg cggtctgcac ccgttcccgt 600
ggcgcggcga aagcgaatat gacgtattaa gcgtcgggca ttcatcaacc tccatcagtg 660
ccggaattgg tattgcggtt gctgccgaaa aagaaggcaa aaatcgccgc accgtctgtg 720
tcattggcga tggcgcgatt accgcaggca tggcgtttga agcgatgaat cacgcgggcg 780
atatccgtcc tgatatgctg gtgattctca acgacaatga aatgtcgatt tccgaaaatg 840
tcggcgcgct caacaaccat ctggcacagc tgctttccgg taagctttac tcttcactgc 900
gcgaaggcgg gaaaaaagtt ttctctggcg tgccgccaat taaagagctg ctcaaacgca 960
ccgaagaaca tattaaaggc atggtagtgc ctggcacgtt gtttgaagag ctgggcttta 1020
actacatcgg cccggtggac ggtcacgatg tgctggggct tatcaccacg ctaaagaaca 1080
tgcgcgacct gaaaggcccg cagttcctgc atatcatgac caaaaaaggt cgtggttatg 1140
aaccggcaga aaaagacccg atcactttcc acgccgtgcc taaatttgat ccctccagcg 1200
gttgtttgcc gaaaagtagc ggcggtttgc cgagctattc aaaaatcttt ggcgactggt 1260
tgtgcgaaac ggcagcgaaa gacaacaagc tgatggcgat tactccggcg atgcgtgaag 1320
gttccggcat ggtcgagttt tcacgtaaat tcccggatcg ctacttcgac gtggcaattg 1380
ccgagcaaca cgcggtgacc tttgctgcgg gtctggcgat tggtgggtac aaacccattg 1440
tcgcgattta ctccactttc ctgcaacgcg cctatgatca ggtgctgcat gacgtggcga 1500
ttcaaaagct tccggtcctg ttcgccatcg accgcgcggg cattgttggt gctgacggtc 1560
aaacccatca gggtgctttt gatctctctt acctgcgctg cataccggaa atggtcatta 1620
tgaccccgag cgatgaaaac gaatgtcgcc agatgctcta taccggctat cactataacg 1680
atggcccgtc agcggtgcgc tacccgcgtg gcaacgcggt cggcgtggaa ctgacgccgc 1740
tggaaaaact accaattggc aaaggcattg tgaagcgtcg tggcgagaaa ctggcgatcc 1800
ttaactttgg tacgctgatg ccagaagcgg cgaaagtcgc cgaatcgctg aacgccacgc 1860
tggtcgatat gcgttttgtg aaaccgcttg atgaagcgtt aattctggaa atggccgcca 1920
gccatgaagc gctggtcacc gtagaagaaa acgccattat gggcggcgca ggcagcggcg 1980
tgaacgaagt gctgatggcc catcgtaaac cagtacccgt gctgaacatt ggcctgccgg 2040
acttctttat tccgcaagga actcaggaag aaatgcgcgc cgaactcggc ctcgatgccg 2100
ctggtatgga agccaaaatc aaggcctggc tggcataagg taccacacag gaaacagacc 2160
atggaattcg agctcatgaa gcaactcacc attctgggct cgaccggctc gattggttgc 2220
agcacgctgg acgtggtgcg ccataatccc gaacacttcc gcgtagttgc gctggtggca 2280
ggcaaaaatg tcactcgcat ggtagaacag tgcctggaat tctctccccg ctatgccgta 2340
atggacgatg aagcgagtgc gaaacttctt aaaacgatgc tacagcaaca gggtagccgc 2400
accgaagtct taagtgggca acaagccgct tgcgatatgg cagcgcttga ggatgttgat 2460
caggtgatgg cagccattgt tggcgctgct gggctgttac ctacgcttgc tgcgatccgc 2520
gcgggtaaaa ccattttgct ggccaataaa gaatcactgg ttacctgcgg acgtctgttt 2580
atggacgccg taaagcagag caaagcgcaa ttgttaccgg tcgatagcga acataacgcc 2640
atttttcaga gtttaccgca acctatccag cataatctgg gatacgctga ccttgagcaa 2700
aatggcgtgg tgtccatttt acttaccggg tctggtggcc ctttccgtga gacgccattg 2760
cgcgatttgg caacaatgac gccggatcaa gcctgccgtc atccgaactg gtcgatgggg 2820
cgtaaaattt ctgtcgattc ggctaccatg atgaacaaag gtctggaata cattgaagcg 2880
cgttggctgt ttaacgccag cgccagccag atggaagtgc tgattcaccc gcagtcagtg 2940
attcactcaa tggtgcgcta tcaggacggc agtgttctgg cgcagctggg ggaaccggat 3000
atgcgtacgc caattgccca caccatggca tggccgaatc gcgtgaactc tggcgtgaag 3060
ccgctcgatt tttgcaaact aagtgcgttg acatttgccg caccggatta tgatcgttat 3120
ccatgcctga aactggcgat ggaggcgttc gaacaaggcc aggcagcgac gacagcattg 3180
aatgccgcaa acgaaatcac cgttgctgct tttcttgcgc aacaaatccg ctttacggat 3240
atcgctgcgt tgaatttatc cgtactggaa aaaatggata tgcgcgaacc acaatgtgtg 3300
gacgatgtgt tatctgttga tgcgaacgcg cgtgaagtcg ccagaaaaga ggtgatgcgt 3360
ctcgcaagct gatctagagc gggggatcca ctagttctag agtcgacctg caggcatgca 3420
agcttggctg ttttggcgga tgagagaaga ttttcagcct gatacagatt aaatcagaac 3480
gcagaagcgg tctgataaaa cagaatttgc ctggcggcag tagcgcggtg gtcccacctg 3540
accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc 3600
atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 3660
gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg 3720
ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca 3780
taaactgcca ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt 3840
ctacaaactc tttttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac 3900
aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt 3960
tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag 4020
aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg 4080
aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa 4140
tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc 4200
aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag 4260
tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa 4320
ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc 4380
taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg 4440
agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctaca gcaatggcaa 4500
caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa 4560
tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg 4620
gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag 4680
cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg 4740
caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt 4800
ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt 4860
aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac 4920
gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 4980
atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 5040
tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 5100
gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga 5160
actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 5220
gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 5280
agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 5340
ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 5400
aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 5460
cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 5520
gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 5580
cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 5640
cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca 5700
gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt 5760
attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa 5820
tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt 5880
catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct 5940
cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt 6000
ttcaccgtca tcaccgaaac gcgcgaggca gcagatcaat tcgcgcgcga aggcgaagcg 6060
gcatgcattt acgttgacac catcgaatgg tgcaaaacct ttcgcggtat ggcatgatag 6120
cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc 6180
gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac 6240
gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc 6300
aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc 6360
agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa 6420
ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg 6480
gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat 6540
gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat 6600
gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg tacgcgactg 6660
ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta 6720
agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa 6780
attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc 6840
atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg 6900
gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggatatctcg 6960
gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt aaccaccatc 7020
aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag 7080
ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc 7140
ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg 7200
gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta 7260
gcgcgaattg atctg 7275
<210> 90
<211> 6798
<212> DNA
<213> Artificial Sequence
<220>
<223> pT-ispA-MrBBS
<400> 90
gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc 60
ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc 120
gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc 180
tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga 240
taacaatttc acacaggaaa cagaccatgg aattcatgga ctttccgcag caactcgaag 300
cctgcgttaa gcaggccaac caggcgctga gccgttttat cgccccactg ccctttcaga 360
acactcccgt ggtcgaaacc atgcagtatg gcgcattatt aggtggtaag cgcctgcgac 420
ctttcctggt ttatgccacc ggtcatatgt tcggcgttag cacaaacacg ctggacgcac 480
ccgctgccgc cgttgagtgt atccacgctt actcattaat tcatgatgat ttaccggcaa 540
tggatgatga cgatctgcgt cgcggtttgc caacctgcca tgtgaagttt ggcgaagcaa 600
acgcgattct cgctggcgac gctttacaaa cgctggcgtt ctcgatttta agcgatgccg 660
atatgccgga agtgtcggac cgcgacagaa tttcgatgat ttctgaactg gcgagcgcca 720
gtggtattgc cggaatgtgc ggtggtcagg cattagattt agacgcggaa ggcaaacacg 780
tacctctgga cgcgcttgag cgtattcatc gtcataaaac cggcgcattg attcgcgccg 840
ccgttcgcct tggtgcatta agcgccggag ataaaggacg tcgtgctctg ccggtactcg 900
acaagtatgc agagagcatc ggccttgcct tccaggttca ggatgacatc ctggatgtgg 960
tgggagatac tgcaacgttg ggaaaacgcc agggtgccga ccagcaactt ggtaaaagta 1020
cctaccctgc acttctgggt cttgagcaag cccggaagaa agcccgggat ctgatcgacg 1080
atgcccgtca gtcgctgaaa caactggctg aacagtcact cgatacctcg gcactggaag 1140
cgctagcgga ctacatcatc cagcgtaata aataaggatc caaggagata tatcaaatgt 1200
caaccctgtc agtctccacg ccgtccttct catcgtcgcc gctgtcctca gtgaacaaaa 1260
atagcacgaa acaacacgtt acgcgcaata gtgtgatctt tcatgattcc atttggggcg 1320
accagttcct ggaatacaaa gaaaaattca acgttgcaac cgaaaaacaa ctgattgaag 1380
aactgaaaga agaagtccgc aatgaactga tgatccgtgc gtgcaacgaa gccagtcgct 1440
atattaaact gatccagctg attgatgtgg ttgaacgtct gggcctggcc taccactttg 1500
aaaaagaaat cgaagaatcc ctgcaacata tttatgttac ctacggtcac aaatggacga 1560
actacaacaa catcgaaagc ctgtctctgt ggtttcgcct gctgcgtcag aacggtttta 1620
atgtcagctc tgatatcttc gaaaaccata ttgacgaaaa aggcaatttc caagaaagtc 1680
tgtgcaacga tccgcaaggc atgctggcac tgtatgaagc ggcctacatg cgcgtggaag 1740
gcgaaattat cctggacaaa gctctggaat ttaccaaact gcacctgggt attatcagca 1800
acgatccgtc ttgtgacagt tccctgcgta cggaaattaa acaggcgctg aaacagccgc 1860
tgcgtcgccg tctgccgcgt ctggaagcag ttcgttatat tgctatctac cagcaaaaag 1920
cgagtcattc cgaagtcctg ctgaaactgg ccaaactgga tttcaatgtg ctgcaggaaa 1980
tgcacaaaga cgaactgtca caaatttgta aatggtggaa agacctggac atccgtaaca 2040
aactgccgta tgttcgcgat cgtctgattg aaggctattt ttggattctg ggtatttact 2100
tcgaaccgca gcattcgcgc acccgtatgt ttctgatgaa aacgtgcatg tggctgatcg 2160
tcctggatga cacctttgat aattatggca cgtacgaaga actggaaatt ttcacccagg 2220
cggtggaacg ctggagcatc acgtgtctgg atgaactgcc ggaatacatg aaactgatct 2280
accatgaaca gttccgtgtg caccaagaaa tggaagaatc tctggaaaaa gaaggtaaag 2340
cataccagat ccattacatc aaagaaatgg ctaaagaagg cacccgctct ctgctgctgg 2400
aagcgaaatg gctgaaagaa ggttatatgc cgacgctgga tgaatacctg tcaaactcgc 2460
tggttacctg cggctatgca ctgatgacgg ctcgctcata cgttgcccgt gatgacggta 2520
ttgtcaccga agatgcgttt aaatgggtgg ccacgcaccc gccgatcgtt aaagcagctt 2580
gtaaaattct gcgtctgatg gatgacatcg cgacccataa agaagaacag gaacgcggcc 2640
acatcgcctc atcgattgaa tgctatcgta aagaaaccgg tgcatctgaa gaagaagcgt 2700
gtatggattt tctgaaacag gtcgaagacg gctggaaagt gatcaatcaa gaatcactga 2760
tgccgaccga tgtgccgttc ccgctgctga tcccggcgat taacctggcc cgcgtttcgg 2820
acacgctgta taaagataac gacggttaca atcatgctga taaagaagtg attggttaca 2880
ttaaaagtct gtttgtccac ccgatgattg tgtgaagatc ttaggtcgac ctgcaggcat 2940
gcaagcttgg ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag 3000
aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac 3060
ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc 3120
cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac 3180
tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg 3240
ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg 3300
ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg 3360
tttctacaaa ctctttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 3420
gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 3480
atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 3540
cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 3600
tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 3660
caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg 3720
ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 3780
cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 3840
taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 3900
agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 3960
cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct acagcaatgg 4020
caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 4080
taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 4140
ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 4200
cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 4260
aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 4320
attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 4380
tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 4440
aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 4500
gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 4560
cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 4620
gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca 4680
agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 4740
ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 4800
cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 4860
acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 4920
gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 4980
ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 5040
agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 5100
cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 5160
tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 5220
gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc 5280
ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta 5340
caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg 5400
ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct 5460
gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag 5520
gttttcaccg tcatcaccga aacgcgcgag gcagcagatc aattcgcgcg cgaaggcgaa 5580
gcggcatgca tttacgttga caccatcgaa tggtgcaaaa cctttcgcgg tatggcatga 5640
tagcgcccgg aagagagtca attcagggtg gtgaatgtga aaccagtaac gttatacgat 5700
gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc 5760
cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt 5820
cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc 5880
tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat 5940
caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa 6000
gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg 6060
gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt 6120
gatgtctctg accagacacc catcaacagt attattttct cccatgaaga cggtacgcga 6180
ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca 6240
ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat 6300
caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa 6360
accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag 6420
atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc 6480
tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc 6540
atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct 6600
cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc 6660
accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 6720
ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag 6780
ttagcgcgaa ttgatctg 6798
<210> 91
<211> 8655
<212> DNA
<213> Artificial Sequence
<220>
<223> pTAS-dxs
<400> 91
tcgacctgca ggcatgcaag cttggctgtt ttggcggatg agagaagatt ttcagcctga 60
tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct ggcggcagta 120
gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg 180
gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag 240
gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg 300
agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg 360
cgggcaggac gcccgccata aactgccagg catcaaatta agcagaaggc catcctgacg 420
gatggccttt ttgcgtttct acaaactctt tttgtttatt tttctaaata cattcaaata 480
tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga 540
gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc 600
ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg 660
cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc 720
ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat 780
cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact 840
tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat 900
tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga 960
tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc 1020
ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga 1080
tgcctacagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag 1140
cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc 1200
gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt 1260
ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct 1320
acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg 1380
cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg 1440
atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca 1500
tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga 1560
tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 1620
aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 1680
aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt 1740
taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 1800
taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 1860
agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 1920
tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 1980
cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 2040
agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 2100
gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 2160
aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 2220
tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag 2280
ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg 2340
aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat 2400
ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct 2460
atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc 2520
ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag 2580
ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc agatcaattc 2640
gcgcgcgaag gcgaagcggc atgcatttac gttgacacca tcgaatggtg caaaaccttt 2700
cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca 2760
gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg 2820
gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg 2880
gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg 2940
attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt 3000
aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc 3060
gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc 3120
attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt 3180
ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat 3240
gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg 3300
ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa 3360
tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg 3420
tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg 3480
gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc 3540
gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc 3600
ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc 3660
ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg 3720
gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc 3780
gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 3840
cgcaattaat gtgagttagc gcgaattgat ctggtttgac agcttatcat cgactgcacg 3900
gtgcaccaat gcttctggcg tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc 3960
gtaaatcact gcataattcg tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt 4020
gcgccgacat cataacggtt ctggcaaata ttctgaaatg agctgttgac aattaatcat 4080
ccggctcgta taatgtgtgg aattgtgagc ggataacaat ttcacacagg aggtaataaa 4140
tatggaattc atggactttc cgcagcaact cgaagcctgc gttaagcagg ccaaccaggc 4200
gctgagccgt tttatcgccc cactgccctt tcagaacact cccgtggtcg aaaccatgca 4260
gtatggcgca ttattaggtg gtaagcgcct gcgacctttc ctggtttatg ccaccggtca 4320
tatgttcggc gttagcacaa acacgctgga cgcacccgct gccgccgttg agtgtatcca 4380
cgcttactca ttaattcatg atgatttacc ggcaatggat gatgacgatc tgcgtcgcgg 4440
tttgccaacc tgccatgtga agtttggcga agcaaacgcg attctcgctg gcgacgcttt 4500
acaaacgctg gcgttctcga ttttaagcga tgccgatatg ccggaagtgt cggaccgcga 4560
cagaatttcg atgatttctg aactggcgag cgccagtggt attgccggaa tgtgcggtgg 4620
tcaggcatta gatttagacg cggaaggcaa acacgtacct ctggacgcgc ttgagcgtat 4680
tcatcgtcat aaaaccggcg cattgattcg cgccgccgtt cgccttggtg cattaagcgc 4740
cggagataaa ggacgtcgtg ctctgccggt actcgacaag tatgcagaga gcatcggcct 4800
tgccttccag gttcaggatg acatcctgga tgtggtggga gatactgcaa cgttgggaaa 4860
acgccagggt gccgaccagc aacttggtaa aagtacctac cctgcacttc tgggtcttga 4920
gcaagcccgg aagaaagccc gggatctgat cgacgatgcc cgtcagtcgc tgaaacaact 4980
ggctgaacag tcactcgata cctcggcact ggaagcgcta gcggactaca tcatccagcg 5040
taataaataa ggatccaaaa aggaggtaat aaaccatgtc aactcaacaa gtttcatcag 5100
agaacattgt tcgtaacgct gcgaatttcc atcctaatat atggggaaac catttcctca 5160
catgtccttc tcagacgatt gatagttgga ctcaacagca ccacaaagaa ctgaaagaag 5220
aggtgaggaa aatgatggtg tctgatgcaa ataaacctgc ccagagattg cgcttgattg 5280
atactgtcca aaggctaggt gtggcttacc actttgaaaa ggagattgat gatgcattgg 5340
agaaaatagg tcatgaccct tttgatgata aagatgatct ctacattgtc tctctttgtt 5400
ttcgattgct gaggcagcat ggaattaaga tatcatgtga tgtgtttgag aagtttaaag 5460
atgacgatgg aaaattcaag gcatcattga tgaatgatgt tcaaggcatg ctaagtttat 5520
atgaggcagc acacctagcc attcacggag aagatatttt agatgaagca attgttttca 5580
cgaccactca ccttaagtca acggtatcta attctcctgt aaactctact tttgctgaac 5640
aaatacgtca ttctctcaga gttcctctcc gtaaagctgt acctaggtta gagtcgaggt 5700
atttcttgga tatctattca agagatgatt tgcacgataa aactttgctc aatttcgcaa 5760
agttagactt taatatacta caagcaatgc accagaagga agcaagtgag atgaccaggt 5820
ggtggagaga ttttgacttc cttaaaaagc tgccttatat aagagacaga gtcgtggagc 5880
tatatttttg gattctggtg ggagtgtctt atcagcccaa attcagcact ggtagaattt 5940
ttttgtccaa aataatatgc cttgagaccc tcgtagatga tacatttgac gcctacggta 6000
cttttgacga gctcgcaatc tttactgaag cagttacaag atgggacctt ggccacagag 6060
atgcactacc agaatacatg aaattcattt tcaagacact cattgatgtc tacagtgaag 6120
ctgagcaaga actggcaaag gaagggagat catacagcat acactatgca atacgatcgt 6180
tccaagaact agttatgaag tacttctgcg aagccaagtg gttaaataaa ggttatgttc 6240
cgagcctgga cgattataaa tcagtttcat taagaagtat cggtttttta ccgatagcgg 6300
tagcttcctt cgttttcatg ggtgatattg caactaagga ggtctttgaa tgggaaatga 6360
ataaccctaa gatcataata gccgcagaaa cgattttcag attcctggat gacatagcag 6420
gccataggtt tgagcaaaag agagaacata gtccatcagc tattgaatgc tacaagaatc 6480
aacatggagt gtctgaggaa gaggcagtta aagcgttgtc gttagaagtt gctaatagtt 6540
ggaaagatat aaatgaggag ctgcttctca acccaatggc tattccttta cctctgcttc 6600
aggtgattct tgatctctca cgttcggccg attttatgta cggtaatgct caagatcgct 6660
tcacgcattc aacgatgatg aaagaccaag ttgatttggt gctgaaggac cccgttaagc 6720
ttgacgatta aagatctgtg tggaattgtg agcggataac aatttcacac aggaaacaga 6780
ccatggaatt catgagtttt gatattgcca aatacccgac cctggcactg gtcgactcca 6840
cccaggagtt acgactgttg ccgaaagaga gtttaccgaa actctgcgac gaactgcgcc 6900
gctatttact cgacagcgtg agccgttcca gcgggcactt cgcctccggg ctgggcacgg 6960
tcgaactgac cgtggcgctg cactatgtct acaacacccc gtttgaccaa ttgatttggg 7020
atgtggggca tcaggcttat ccgcataaaa ttttgaccgg acgccgcgac aaaatcggca 7080
ccatccgtca gaaaggcggt ctgcacccgt tcccgtggcg cggcgaaagc gaatatgacg 7140
tattaagcgt cgggcattca tcaacctcca tcagtgccgg aattggtatt gcggttgctg 7200
ccgaaaaaga aggcaaaaat cgccgcaccg tctgtgtcat tggcgatggc gcgattaccg 7260
caggcatggc gtttgaagcg atgaatcacg cgggcgatat ccgtcctgat atgctggtga 7320
ttctcaacga caatgaaatg tcgatttccg aaaatgtcgg cgcgctcaac aaccatctgg 7380
cacagctgct ttccggtaag ctttactctt cactgcgcga aggcgggaaa aaagttttct 7440
ctggcgtgcc gccaattaaa gagctgctca aacgcaccga agaacatatt aaaggcatgg 7500
tagtgcctgg cacgttgttt gaagagctgg gctttaacta catcggcccg gtggacggtc 7560
acgatgtgct ggggcttatc accacgctaa agaacatgcg cgacctgaaa ggcccgcagt 7620
tcctgcatat catgaccaaa aaaggtcgtg gttatgaacc ggcagaaaaa gacccgatca 7680
ctttccacgc cgtgcctaaa tttgatccct ccagcggttg tttgccgaaa agtagcggcg 7740
gtttgccgag ctattcaaaa atctttggcg actggttgtg cgaaacggca gcgaaagaca 7800
acaagctgat ggcgattact ccggcgatgc gtgaaggttc cggcatggtc gagttttcac 7860
gtaaattccc ggatcgctac ttcgacgtgg caattgccga gcaacacgcg gtgacctttg 7920
ctgcgggtct ggcgattggt gggtacaaac ccattgtcgc gatttactcc actttcctgc 7980
aacgcgccta tgatcaggtg ctgcatgacg tggcgattca aaagcttccg gtcctgttcg 8040
ccatcgaccg cgcgggcatt gttggtgctg acggtcaaac ccatcagggt gcttttgatc 8100
tctcttacct gcgctgcata ccggaaatgg tcattatgac cccgagcgat gaaaacgaat 8160
gtcgccagat gctctatacc ggctatcact ataacgatgg cccgtcagcg gtgcgctacc 8220
cgcgtggcaa cgcggtcggc gtggaactga cgccgctgga aaaactacca attggcaaag 8280
gcattgtgaa gcgtcgtggc gagaaactgg cgatccttaa ctttggtacg ctgatgccag 8340
aagcggcgaa agtcgccgaa tcgctgaacg ccacgctggt cgatatgcgt tttgtgaaac 8400
cgcttgatga agcgttaatt ctggaaatgg ccgccagcca tgaagcgctg gtcaccgtag 8460
aagaaaacgc cattatgggc ggcgcaggca gcggcgtgaa cgaagtgctg atggcccatc 8520
gtaaaccagt acccgtgctg aacattggcc tgccggactt ctttattccg caaggaactc 8580
aggaagaaat gcgcgccgaa ctcggcctcg atgccgctgg tatggaagcc aaaatcaagg 8640
cctggctggc ataac 8655
<210> 92
<211> 8656
<212> DNA
<213> Artificial Sequence
<220>
<223> pTAB-idi-dxr
<400> 92
gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc 60
ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc 120
gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc 180
tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga 240
taacaatttc acacaggaaa cagaccatgg aattcgagct cggtacccgg ggatccgatc 300
cgggaggttt tcagtaatgg actttccgca gcaactcgaa gcctgcgtta agcaggccaa 360
ccaggcgctg agccgtttta tcgccccact gccctttcag aacactcccg tggtcgaaac 420
catgcagtat ggcgcattat taggtggtaa gcgcctgcga cctttcctgg tttatgccac 480
cggtcatatg ttcggcgtta gcacaaacac gctggacgca cccgctgccg ccgttgagtg 540
tatccacgct tactcattaa ttcatgatga tttaccggca atggatgatg acgatctgcg 600
tcgcggtttg ccaacctgcc atgtgaagtt tggcgaagca aacgcgattc tcgctggcga 660
cgctttacaa acgctggcgt tctcgatttt aagcgatgcc gatatgccgg aagtgtcgga 720
ccgcgacaga atttcgatga tttctgaact ggcgagcgcc agtggtattg ccggaatgtg 780
cggtggtcag gcattagatt tagacgcgga aggcaaacac gtacctctgg acgcgcttga 840
gcgtattcat cgtcataaaa ccggcgcatt gattcgcgcc gccgttcgcc ttggtgcatt 900
aagcgccgga gataaaggac gtcgtgctct gccggtactc gacaagtatg cagagagcat 960
cggccttgcc ttccaggttc aggatgacat cctggatgtg gtgggagata ctgcaacgtt 1020
gggaaaacgc cagggtgccg accagcaact tggtaaaagt acctaccctg cacttctggg 1080
tcttgagcaa gcccggaaga aagcccggga tctgatcgac gatgcccgtc agtcgctgaa 1140
acaactggct gaacagtcac tcgatacctc ggcactggaa gcgctagcgg actacatcat 1200
ccagcgtaat aaataagatc cataaaggag gtaagaatgt caaccctgtc agtctccacg 1260
ccgtccttct catcgtcgcc gctgtcctca gtgaacaaaa atagcacgaa acaacacgtt 1320
acgcgcaata gtgtgatctt tcatgattcc atttggggcg accagttcct ggaatacaaa 1380
gaaaaattca acgttgcaac cgaaaaacaa ctgattgaag aactgaaaga agaagtccgc 1440
aatgaactga tgatccgtgc gtgcaacgaa gccagtcgct atattaaact gatccagctg 1500
attgatgtgg ttgaacgtct gggcctggcc taccactttg aaaaagaaat cgaagaatcc 1560
ctgcaacata tttatgttac ctacggtcac aaatggacga actacaacaa catcgaaagc 1620
ctgtctctgt ggtttcgcct gctgcgtcag aacggtttta atgtcagctc tgatatcttc 1680
gaaaaccata ttgacgaaaa aggcaatttc caagaaagtc tgtgcaacga tccgcaaggc 1740
atgctggcac tgtatgaagc ggcctacatg cgcgtggaag gcgaaattat cctggacaaa 1800
gctctggaat ttaccaaact gcacctgggt attatcagca acgatccgtc ttgtgacagt 1860
tccctgcgta cggaaattaa acaggcgctg aaacagccgc tgcgtcgccg tctgccgcgt 1920
ctggaagcag ttcgttatat tgctatctac cagcaaaaag cgagtcattc cgaagtcctg 1980
ctgaaactgg ccaaactgga tttcaatgtg ctgcaggaaa tgcacaaaga cgaactgtca 2040
caaatttgta aatggtggaa agacctggac atccgtaaca aactgccgta tgttcgcgat 2100
cgtctgattg aaggctattt ttggattctg ggtatttact tcgaaccgca gcattcgcgc 2160
acccgtatgt ttctgatgaa aacgtgcatg tggctgatcg tcctggatga cacctttgat 2220
aattatggca cgtacgaaga actggaaatt ttcacccagg cggtggaacg ctggagcatc 2280
acgtgtctgg atgaactgcc ggaatacatg aaactgatct accatgaaca gttccgtgtg 2340
caccaagaaa tggaagaatc tctggaaaaa gaaggtaaag cataccagat ccattacatc 2400
aaagaaatgg ctaaagaagg cacccgctct ctgctgctgg aagcgaaatg gctgaaagaa 2460
ggttatatgc cgacgctgga tgaatacctg tcaaactcgc tggttacctg cggctatgca 2520
ctgatgacgg ctcgctcata cgttgcccgt gatgacggta ttgtcaccga agatgcgttt 2580
aaatgggtgg ccacgcaccc gccgatcgtt aaagcagctt gtaaaattct gcgtctgatg 2640
gatgacatcg cgacccataa agaagaacag gaacgcggcc acatcgcctc atcgattgaa 2700
tgctatcgta aagaaaccgg tgcatctgaa gaagaagcgt gtatggattt tctgaaacag 2760
gtcgaagacg gctggaaagt gatcaatcaa gaatcactga tgccgaccga tgtgccgttc 2820
ccgctgctga tcccggcgat taacctggcc cgcgtttcgg acacgctgta taaagataac 2880
gacggttaca atcatgctga taaagaagtg attggttaca ttaaaagtct gtttgtccac 2940
ccgatgattg tgtgaagatc ctgaggaggt aacgtatgca aacggaacac gtcattttat 3000
tgaatgcaca gggagttccc acgggtacgc tggaaaagta tgccgcacac acggcagaca 3060
cccgcttaca tctcgcgttc tccagttggc tgtttaatgc caaaggacaa ttattagtta 3120
cccgccgcgc actgagcaaa aaagcatggc ctggcgtgtg gactaactcg gtttgtgggc 3180
acccacaact gggagaaagc aacgaagacg cagtgatccg ccgttgccgt tatgagcttg 3240
gcgtggaaat tacgcctcct gaatctatct atcctgactt tcgctaccgc gccaccgatc 3300
cgagtggcat tgtggaaaat gaagtgtgtc cggtatttgc cgcacgcacc actagtgcgt 3360
tacagatcaa tgatgatgaa gtgatggatt atcaatggtg tgatttagca gatgtattac 3420
acggtattga tgccacgccg tgggcgttca gtccgtggat ggtgatgcag gcgacaaatc 3480
gcgaagccag aaaacgatta tctgcattta cccagcttaa ataagatctt agagtcgacg 3540
tgtggaattg tgagcggata acaatttcac acaggaaaca gaccatggaa ttcgagctca 3600
tgaagcaact caccattctg ggctcgaccg gctcgattgg ttgcagcacg ctggacgtgg 3660
tgcgccataa tcccgaacac ttccgcgtag ttgcgctggt ggcaggcaaa aatgtcactc 3720
gcatggtaga acagtgcctg gaattctctc cccgctatgc cgtaatggac gatgaagcga 3780
gtgcgaaact tcttaaaacg atgctacagc aacagggtag ccgcaccgaa gtcttaagtg 3840
ggcaacaagc cgcttgcgat atggcagcgc ttgaggatgt tgatcaggtg atggcagcca 3900
ttgttggcgc tgctgggctg ttacctacgc ttgctgcgat ccgcgcgggt aaaaccattt 3960
tgctggccaa taaagaatca ctggttacct gcggacgtct gtttatggac gccgtaaagc 4020
agagcaaagc gcaattgtta ccggtcgata gcgaacataa cgccattttt cagagtttac 4080
cgcaacctat ccagcataat ctgggatacg ctgaccttga gcaaaatggc gtggtgtcca 4140
ttttacttac cgggtctggt ggccctttcc gtgagacgcc attgcgcgat ttggcaacaa 4200
tgacgccgga tcaagcctgc cgtcatccga actggtcgat ggggcgtaaa atttctgtcg 4260
attcggctac catgatgaac aaaggtctgg aatacattga agcgcgttgg ctgtttaacg 4320
ccagcgccag ccagatggaa gtgctgattc acccgcagtc agtgattcac tcaatggtgc 4380
gctatcagga cggcagtgtt ctggcgcagc tgggggaacc ggatatgcgt acgccaattg 4440
cccacaccat ggcatggccg aatcgcgtga actctggcgt gaagccgctc gatttttgca 4500
aactaagtgc gttgacattt gccgcaccgg attatgatcg ttatccatgc ctgaaactgg 4560
cgatggaggc gttcgaacaa ggccaggcag cgacgacagc attgaatgcc gcaaacgaaa 4620
tcaccgttgc tgcttttctt gcgcaacaaa tccgctttac ggatatcgct gcgttgaatt 4680
tatccgtact ggaaaaaatg gatatgcgcg aaccacaatg tgtggacgat gtgttatctg 4740
ttgatgcgaa cgcgcgtgaa gtcgccagaa aagaggtgat gcgtctcgca agctgatcta 4800
aagcttggct gttttggcgg atgagagaag attttcagcc tgatacagat taaatcagaa 4860
cgcagaagcg gtctgataaa acagaatttg cctggcggca gtagcgcggt ggtcccacct 4920
gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc 4980
catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg 5040
ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc 5100
gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc 5160
ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttgcgtt 5220
tctacaaact ctttttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga 5280
caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat 5340
ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca 5400
gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc 5460
gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca 5520
atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg 5580
caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca 5640
gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata 5700
accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag 5760
ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg 5820
gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctac agcaatggca 5880
acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta 5940
atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct 6000
ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca 6060
gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag 6120
gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat 6180
tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt 6240
taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 6300
cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 6360
gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 6420
gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 6480
agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 6540
aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 6600
agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 6660
cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 6720
accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 6780
aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 6840
ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 6900
cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 6960
gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 7020
tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 7080
agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg 7140
tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca 7200
atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg 7260
tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc 7320
tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt 7380
tttcaccgtc atcaccgaaa cgcgcgaggc agcagatcaa ttcgcgcgcg aaggcgaagc 7440
ggcatgcatt tacgttgaca ccatcgaatg gtgcaaaacc tttcgcggta tggcatgata 7500
gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt 7560
cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca 7620
cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc 7680
caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc 7740
cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca 7800
actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc 7860
ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga 7920
tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga 7980
tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact 8040
gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt 8100
aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca 8160
aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac 8220
catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat 8280
ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc 8340
ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat 8400
caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca 8460
gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac 8520
cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct 8580
ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt 8640
agcgcgaatt gatctg 8656
<210> 93
<211> 9789
<212> DNA
<213> Artificial Sequence
<220>
<223> pSNAK(-E)-dxs
<400> 93
gatctcatta ggtagccgtt tcaggcacaa ttgggattga taaaattagt ttttttgtgc 60
ccccttatta tattgatatg acggcactgg ctgaagccag aaatgtagac cctggaaaat 120
ttcatattgg tattgggcaa gaccaaatgg cggtgaaccc aatcagccaa gatattgtga 180
catttgcagc caatgccgca gaagcgatct tgaccaaaga agataaagag gccattgata 240
tggtgattgt cgggactgag tccagtatcg atgagtcaaa agcggccgca gttgtcttac 300
atcgtttaat ggggattcaa cctttcgctc gctctttcga aatcaaggaa gcttgttacg 360
gagcaacagc aggcttacag ttagctaaga atcacgtagc cttacatcca gataaaaaag 420
tcttggttgt agcagcagat attgcaaaat atggattaaa ttctggcggt gagcctacac 480
aaggagctgg ggcggttgca atgttagttg ctagtgaacc gcgcatcttg gctttaaaag 540
aggataatgt gatgctgacg caagatatct atgacttttg gcgtccaaca ggccatccgt 600
atcctatggt cgatggtcct ttgtcaaacg aaacctacat ccaatctttt gcccaagtct 660
gggatgaaca taaaaaaaga accggtcttg attttgcaga ttatgatgct ttagcgttcc 720
atattcctta cacaaaaatg ggcaaaaaag ccttattagc aaaaatctcc gaccaaactg 780
aagcagaaca ggaacgaatt ttagcccgtt atgaagaaag catcatctat agtcgtcgcg 840
taggaaactt gtatacgggt tcactttatc tgggactcat ttccctttta gaaaatgcaa 900
cgactttaac cgcaggcaat caaattgggt tattcagtta tggttctggt gctgtcgctg 960
aatttttcac tggtgaatta gtagctggtt atcaaaatca tttacaaaaa gaaactcatt 1020
tagcactgct agataatcgg acagaacttt ctatcgctga atatgaagcc atgtttgcag 1080
aaactttaga cacagatatt gatcaaacgt tagaagatga attaaaatat agtatttctg 1140
ctattaataa taccgttcgc tcttatcgaa actaactgca gcctcgacct gcaggcatgc 1200
aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca 1260
acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg 1320
caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgagctt atcgatgata 1380
agctgtcaaa catgagaatt acaacttata tcgtatgggg ctgacttcag gtgctacatt 1440
tgaagagata aattgcactg aaatctagaa atattttatc tgattaataa gatgatcttc 1500
ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa aacgaaaaaa ccgccttgca 1560
gggcggtttt tcgaaggttc tctgagctac caactctttg aaccgaggta actggcttgg 1620
aggagcgcag tcaccaaaac ttgtcctttc agtttagcct taaccggcgc atgacttcaa 1680
gactaactcc tctaaatcaa ttaccagtgg ctgctgccag tggtgctttt gcatgtcttt 1740
ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcggac tgaacggggg 1800
gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg 1860
gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca 1920
ggagagcgca cgagggagcc gccaggggaa acgcctggta tctttatagt cctgtcgggt 1980
ttcgccacca ctgatttgag cgtcagattt cgtgatgctt gtcagggggg cggagcctat 2040
ggaaaaacgg ctttgccgcg gccctctcac ttccctgtta agtatcttcc tggcatcttc 2100
caggaaatct ccgccccgtt cgtaagccat ttccgctcgc cgcagtcgaa cgaccgagcg 2160
tagcgagtca gtgagcgagg aagcggaata tatcctgtat cacatattct gctgacgcac 2220
cggtgcagcc ttttttctcc tgccacatga agcacttcac tgacaccctc atcagtgcca 2280
acatagtaag ccagtataca ctccgctagc gctgatgtcc ggcggtgctt ttgccgttac 2340
gcaccacccc gtcagtagct gaacaggagg gacagctgat agaaacagaa gccactggag 2400
cacctcaaaa acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt 2460
tgcgccgaat aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc 2520
cctgttgata ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac 2580
gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag 2640
ttatcgagat tttcaggagc taaggaagct aaaatgagcc atattcaacg ggaaacgtct 2700
tgctctaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct 2760
cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg 2820
ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg 2880
gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt 2940
actcctgatg atgcatggtt actcaccact gcgatccccg ggaaaacagc attccaggta 3000
ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc 3060
cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc 3120
gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag 3180
cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca 3240
ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg 3300
aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt 3360
gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa 3420
aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag 3480
tttttctaaa gtactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt 3540
ggtgccctta aacgcctggt gctacgcctg aataagtgat aataagcgga tgaatggcag 3600
aaattcgaaa gcaaattcga cccggtcgtc ggttcagggc agggtcgtta aatagccgct 3660
tatgtctatt gctggtttac cggtttattg actaccggaa gcagtgtgac cgtgtgcttc 3720
tcaaatgcct gaggccagtt tgctcaggct ctccccgtgg aggtaataat tgacgatatg 3780
atcatttatt ctgcctccca gagcctgata aaaacggtta gcgcttcgtt aatacagatg 3840
taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc 3900
agggcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg 3960
ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga 4020
tagtcatgcc ccgcgcccac cggaaggagc taccggacag cggtgcggac tgttgtaact 4080
cagaataaga aatgaggccg ctcatggcgt tccaatacgc aaaccgcctc tccccgcgcg 4140
ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga 4200
gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat 4260
gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag 4320
ctatgaccat gattacgaat tcgagctcgg tacccgggga tcctacctga cgctttttat 4380
cgcaactctc tactgtttct ccatacccgt ttttttgggc tagcaggagg aattcaccat 4440
ggtaccaatg acaaaaaaag ttggtgtcgg tcaggcacat agtaagataa ttttaatagg 4500
ggaacatgcg gtcgtttacg gttatcctgc catttccctg cctcttttgg aggtggaggt 4560
gacctgtaag gtagttcctg cagagagtcc ttggcgcctt tatgaggagg ataccttgtc 4620
catggcggtt tatgcctcac tggagtattt gaatatcaca gaagcctgca ttcgttgtga 4680
gattgactcg gctatccctg agaaacgggg gatgggttcg tcagcggcta tcagcatagc 4740
ggccattcgt gcagtatttg actactatca ggctgatctg cctcatgatg tactagaaat 4800
cttggtcaat cgagctgaaa tgattgccca tatgaatcct agtggtttgg atgctaagac 4860
ctgtcttagt gaccaaccta ttcgctttat caagaacgta ggatttacag aacttgagat 4920
ggatttatcc gcctatttgg tgattgccga tacgggtgtt tatggtcata ctcgtgaagc 4980
catccaagtg gttcaaaata agggcaagga tgccctaccg tttttgcatg ccttgggaga 5040
attaacccag caagcagaag ttgcgatttc acaaaaagat gctgaaggac tgggacaaat 5100
cctcagtcaa gcgcatttac atttaaaaga aattggagtc agtagccctg aggcagactt 5160
tttggttgaa acgactctta gccatggtgc tctgggtgcc aagatgagcg gtggtgggct 5220
aggaggttgt atcatagcct tggtaaccaa tttgacacac gcacaagaac tagcagaaag 5280
attagaagag aaaggagctg ttcagacatg gatagagagc ctgtaacagt acgttcctac 5340
gcaaatattg ctattatcaa atattgggga aagaaaaaag aaaaagagat ggtgcctgct 5400
actagcagta tttctctaac tttggaaaat atgtatacag agacgacctt gtcgccttta 5460
ccagccaatg taacagctga cgaattttac atcaatggtc agctacaaaa tgaggtcgag 5520
catgccaaga tgagtaagat tattgaccgt tatcgtccag ctggtgaggg ctttgtccgt 5580
atcgatactc aaaacaatat gcctacggca gcgggcctgt cctcaagttc tagtggtttg 5640
tccgccctgg tcaaggcttg taatgcttat ttcaagcttg gattggatag aagtcagttg 5700
gcacaggaag ccaaatttgc ctcaggctct tcttctcgga gtttttatgg accactagga 5760
gcctgggata aggatagtgg agaaatttac cctgtagaga cagacttgaa actagctatg 5820
attatgttgg tgctagagga caagaaaaaa ccaatctcta gccgtgacgg gatgaaactt 5880
tgtgtggaaa cctcgacgac ttttgacgac tgggttcgtc agtctgagaa ggactatcag 5940
gatatgctga tttatctcaa ggaaaatgat tttgccaaga ttggagaatt aacggagaaa 6000
aatgctctgg ctatgcatgc tacgacaaag actgctagtc cagccttttc ttatctgacg 6060
gatgcctctt atgaggctat ggcctttgtt cgccagcttc gtgagaaagg agaggcctgc 6120
tactttacca tggatgctgg tcccaatgtt aaggtcttct gtcaggagaa agacttggag 6180
catttgtcag aaattttcgg tcagcgttat cgcttgattg tgtcaaaaac aaaggatttg 6240
agtcaagatg attgctgtta aaacttgcgg aaaactctat tgggcaggtg aatatgctat 6300
tttagagcca gggcagttag ctttgataaa ggatattccc atctatatga gggctgagat 6360
tgctttttct gacagctacc gtatctattc agatatgttt gatttcgcag tggacttaag 6420
gcccaatcct gactacagct tgattcaaga aacgattgct ttgatgggag acttcctcgc 6480
tgttcgcggt cagaatttaa gacctttttc cctaaaaatc tgtggcaaaa tggaacgaga 6540
agggaaaaag tttggtctag gttctagtgg cagcgtcgtt gtcttggttg tcaaggcttt 6600
actggctctc tataatcttt cggttgatca gaatctcttg ttcaagctga ctagcgctgt 6660
cttgctcaag cgaggagaca atggttccat gggcgacctt gcctgtattg tggcagagga 6720
tttggttctt taccagtcat ttgatcgcca gaaggcggct gcttggttag aagaagaaaa 6780
cttggcgaca gttctggagc gtgattgggg attttttatc tcacaagtga aaccaacttt 6840
agaatgtgat ttcttagtgg gatggaccaa ggaagtggct gtatcgagtc acatggtcca 6900
gcaaatcaag caaaatatca atcaaaattt tttaagttcc tcaaaagaaa cggtggtttc 6960
tttggtcgaa gccttggagc aggggaaagc cgaaaaagtt atcgagcaag tagaagtagc 7020
cagcaagctt ttagaaggct tgagtacaga tatttacacg cctttgctta gacagttgaa 7080
agaagccagt caagatttgc aggccgttgc caagagtagt ggtgctggtg gtggtgactg 7140
tggcatcgcc ctgagttttg atgcgcaatc ttctcgaaac actttaaaaa atcgttgggc 7200
cgatctgggg attgagctct tatatcaaga aaggatagga catgacgaca aatcgtaatc 7260
tagccccggg aggagagaaa ttatgcaaac ggaacacgtc attttattga atgcacaggg 7320
agttcccacg ggtacgctgg aaaagtatgc cgcacacacg gcagacaccc gcttacatct 7380
cgcgttctcc agttggctgt ttaatgccaa aggacaatta ttagttaccc gccgcgcact 7440
gagcaaaaaa gcatggcctg gcgtgtggac taactcggtt tgtgggcacc cacaactggg 7500
agaaagcaac gaagacgcag tgatccgccg ttgccgttat gagcttggcg tggaaattac 7560
gcctcctgaa tctatctatc ctgactttcg ctaccgcgcc accgatccga gtggcattgt 7620
ggaaaatgaa gtgtgtccgg tatttgccgc acgcaccact agtgcgttac agatcaatga 7680
tgatgaagtg atggattatc aatggtgtga tttagcagat gtattacacg gtattgatgc 7740
cacgccgtgg gcgttcagtc cgtggatggt gatgcaggcg acaaatcgcg aagccagaaa 7800
acgattatct gcatttaccc agcttaaata agcatgcact agagtcgagg aaacagacca 7860
tggagtctcg agtgtggaat tgtgagcgga taacaatttc acacaggaaa cagaccatgg 7920
aattcatgag ttttgatatt gccaaatacc cgaccctggc actggtcgac tccacccagg 7980
agttacgact gttgccgaaa gagagtttac cgaaactctg cgacgaactg cgccgctatt 8040
tactcgacag cgtgagccgt tccagcgggc acttcgcctc cgggctgggc acggtcgaac 8100
tgaccgtggc gctgcactat gtctacaaca ccccgtttga ccaattgatt tgggatgtgg 8160
ggcatcaggc ttatccgcat aaaattttga ccggacgccg cgacaaaatc ggcaccatcc 8220
gtcagaaagg cggtctgcac ccgttcccgt ggcgcggcga aagcgaatat gacgtattaa 8280
gcgtcgggca ttcatcaacc tccatcagtg ccggaattgg tattgcggtt gctgccgaaa 8340
aagaaggcaa aaatcgccgc accgtctgtg tcattggcga tggcgcgatt accgcaggca 8400
tggcgtttga agcgatgaat cacgcgggcg atatccgtcc tgatatgctg gtgattctca 8460
acgacaatga aatgtcgatt tccgaaaatg tcggcgcgct caacaaccat ctggcacagc 8520
tgctttccgg taagctttac tcttcactgc gcgaaggcgg gaaaaaagtt ttctctggcg 8580
tgccgccaat taaagagctg ctcaaacgca ccgaagaaca tattaaaggc atggtagtgc 8640
ctggcacgtt gtttgaagag ctgggcttta actacatcgg cccggtggac ggtcacgatg 8700
tgctggggct tatcaccacg ctaaagaaca tgcgcgacct gaaaggcccg cagttcctgc 8760
atatcatgac caaaaaaggt cgtggttatg aaccggcaga aaaagacccg atcactttcc 8820
acgccgtgcc taaatttgat ccctccagcg gttgtttgcc gaaaagtagc ggcggtttgc 8880
cgagctattc aaaaatcttt ggcgactggt tgtgcgaaac ggcagcgaaa gacaacaagc 8940
tgatggcgat tactccggcg atgcgtgaag gttccggcat ggtcgagttt tcacgtaaat 9000
tcccggatcg ctacttcgac gtggcaattg ccgagcaaca cgcggtgacc tttgctgcgg 9060
gtctggcgat tggtgggtac aaacccattg tcgcgattta ctccactttc ctgcaacgcg 9120
cctatgatca ggtgctgcat gacgtggcga ttcaaaagct tccggtcctg ttcgccatcg 9180
accgcgcggg cattgttggt gctgacggtc aaacccatca gggtgctttt gatctctctt 9240
acctgcgctg cataccggaa atggtcatta tgaccccgag cgatgaaaac gaatgtcgcc 9300
agatgctcta taccggctat cactataacg atggcccgtc agcggtgcgc tacccgcgtg 9360
gcaacgcggt cggcgtggaa ctgacgccgc tggaaaaact accaattggc aaaggcattg 9420
tgaagcgtcg tggcgagaaa ctggcgatcc ttaactttgg tacgctgatg ccagaagcgg 9480
cgaaagtcgc cgaatcgctg aacgccacgc tggtcgatat gcgttttgtg aaaccgcttg 9540
atgaagcgtt aattctggaa atggccgcca gccatgaagc gctggtcacc gtagaagaaa 9600
acgccattat gggcggcgca ggcagcggcg tgaacgaagt gctgatggcc catcgtaaac 9660
cagtacccgt gctgaacatt ggcctgccgg acttctttat tccgcaagga actcaggaag 9720
aaatgcgcgc cgaactcggc ctcgatgccg ctggtatgga agccaaaatc aaggcctggc 9780
tggcataaa 9789
<210> 94
<211> 8938
<212> DNA
<213> Artificial Sequence
<220>
<223> pSNA(-E)-dxsfree
<400> 94
gatctcatta ggtagccgtt tcaggcacaa ttgggattga taaaattagt ttttttgtgc 60
ccccttatta tattgatatg acggcactgg ctgaagccag aaatgtagac cctggaaaat 120
ttcatattgg tattgggcaa gaccaaatgg cggtgaaccc aatcagccaa gatattgtga 180
catttgcagc caatgccgca gaagcgatct tgaccaaaga agataaagag gccattgata 240
tggtgattgt cgggactgag tccagtatcg atgagtcaaa agcggccgca gttgtcttac 300
atcgtttaat ggggattcaa cctttcgctc gctctttcga aatcaaggaa gcttgttacg 360
gagcaacagc aggcttacag ttagctaaga atcacgtagc cttacatcca gataaaaaag 420
tcttggttgt agcagcagat attgcaaaat atggattaaa ttctggcggt gagcctacac 480
aaggagctgg ggcggttgca atgttagttg ctagtgaacc gcgcatcttg gctttaaaag 540
aggataatgt gatgctgacg caagatatct atgacttttg gcgtccaaca ggccatccgt 600
atcctatggt cgatggtcct ttgtcaaacg aaacctacat ccaatctttt gcccaagtct 660
gggatgaaca taaaaaaaga accggtcttg attttgcaga ttatgatgct ttagcgttcc 720
atattcctta cacaaaaatg ggcaaaaaag ccttattagc aaaaatctcc gaccaaactg 780
aagcagaaca ggaacgaatt ttagcccgtt atgaagaaag catcatctat agtcgtcgcg 840
taggaaactt gtatacgggt tcactttatc tgggactcat ttccctttta gaaaatgcaa 900
cgactttaac cgcaggcaat caaattgggt tattcagtta tggttctggt gctgtcgctg 960
aatttttcac tggtgaatta gtagctggtt atcaaaatca tttacaaaaa gaaactcatt 1020
tagcactgct agataatcgg acagaacttt ctatcgctga atatgaagcc atgtttgcag 1080
aaactttaga cacagatatt gatcaaacgt tagaagatga attaaaatat agtatttctg 1140
ctattaataa taccgttcgc tcttatcgaa actaactgca gcctcgacct gcaggcatgc 1200
aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca 1260
acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg 1320
caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgagctt atcgatgata 1380
agctgtcaaa catgagaatt acaacttata tcgtatgggg ctgacttcag gtgctacatt 1440
tgaagagata aattgcactg aaatctagaa atattttatc tgattaataa gatgatcttc 1500
ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa aacgaaaaaa ccgccttgca 1560
gggcggtttt tcgaaggttc tctgagctac caactctttg aaccgaggta actggcttgg 1620
aggagcgcag tcaccaaaac ttgtcctttc agtttagcct taaccggcgc atgacttcaa 1680
gactaactcc tctaaatcaa ttaccagtgg ctgctgccag tggtgctttt gcatgtcttt 1740
ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcggac tgaacggggg 1800
gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg 1860
gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca 1920
ggagagcgca cgagggagcc gccaggggaa acgcctggta tctttatagt cctgtcgggt 1980
ttcgccacca ctgatttgag cgtcagattt cgtgatgctt gtcagggggg cggagcctat 2040
ggaaaaacgg ctttgccgcg gccctctcac ttccctgtta agtatcttcc tggcatcttc 2100
caggaaatct ccgccccgtt cgtaagccat ttccgctcgc cgcagtcgaa cgaccgagcg 2160
tagcgagtca gtgagcgagg aagcggaata tatcctgtat cacatattct gctgacgcac 2220
cggtgcagcc ttttttctcc tgccacatga agcacttcac tgacaccctc atcagtgcca 2280
acatagtaag ccagtataca ctccgctagc gctgatgtcc ggcggtgctt ttgccgttac 2340
gcaccacccc gtcagtagct gaacaggagg gacagctgat agaaacagaa gccactggag 2400
cacctcaaaa acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt 2460
tgcgccgaat aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc 2520
cctgttgata ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac 2580
gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag 2640
ttatcgagat tttcaggagc taaggaagct tttttttaag gcagttattg gtgcccttaa 2700
acgcctggtg ctacgcctga ataagtgata ataagcggat gaatggcaga aattcgaaag 2760
caaattcgac ccggtcgtcg gttcagggca gggtcgttaa atagccgctt atgtctattg 2820
ctggtttacc ggtttattga ctaccggaag cagtgtgacc gtgtgcttct caaatgcctg 2880
aggccagttt gctcaggctc tccccgtgga ggtaataatt gacgatatga tcatttattc 2940
tgcctcccag agcctgataa aaacggttag cgcttcgtta atacagatgt aggtgttcca 3000
cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgcttgt 3060
ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc tgccggcacc 3120
tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc 3180
cgcgcccacc ggaaggagct accggacagc ggtgcggact gttgtaactc agaataagaa 3240
atgaggccgc tcatggcgtt ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 3300
attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 3360
ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc 3420
gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg 3480
attacgaatt cgagctcggt acccggggat cctacctgac gctttttatc gcaactctct 3540
actgtttctc catacccgtt tttttgggct agcaggagga attcaccatg gtaccaatga 3600
caaaaaaagt tggtgtcggt caggcacata gtaagataat tttaataggg gaacatgcgg 3660
tcgtttacgg ttatcctgcc atttccctgc ctcttttgga ggtggaggtg acctgtaagg 3720
tagttcctgc agagagtcct tggcgccttt atgaggagga taccttgtcc atggcggttt 3780
atgcctcact ggagtatttg aatatcacag aagcctgcat tcgttgtgag attgactcgg 3840
ctatccctga gaaacggggg atgggttcgt cagcggctat cagcatagcg gccattcgtg 3900
cagtatttga ctactatcag gctgatctgc ctcatgatgt actagaaatc ttggtcaatc 3960
gagctgaaat gattgcccat atgaatccta gtggtttgga tgctaagacc tgtcttagtg 4020
accaacctat tcgctttatc aagaacgtag gatttacaga acttgagatg gatttatccg 4080
cctatttggt gattgccgat acgggtgttt atggtcatac tcgtgaagcc atccaagtgg 4140
ttcaaaataa gggcaaggat gccctaccgt ttttgcatgc cttgggagaa ttaacccagc 4200
aagcagaagt tgcgatttca caaaaagatg ctgaaggact gggacaaatc ctcagtcaag 4260
cgcatttaca tttaaaagaa attggagtca gtagccctga ggcagacttt ttggttgaaa 4320
cgactcttag ccatggtgct ctgggtgcca agatgagcgg tggtgggcta ggaggttgta 4380
tcatagcctt ggtaaccaat ttgacacacg cacaagaact agcagaaaga ttagaagaga 4440
aaggagctgt tcagacatgg atagagagcc tgtaacagta cgttcctacg caaatattgc 4500
tattatcaaa tattggggaa agaaaaaaga aaaagagatg gtgcctgcta ctagcagtat 4560
ttctctaact ttggaaaata tgtatacaga gacgaccttg tcgcctttac cagccaatgt 4620
aacagctgac gaattttaca tcaatggtca gctacaaaat gaggtcgagc atgccaagat 4680
gagtaagatt attgaccgtt atcgtccagc tggtgagggc tttgtccgta tcgatactca 4740
aaacaatatg cctacggcag cgggcctgtc ctcaagttct agtggtttgt ccgccctggt 4800
caaggcttgt aatgcttatt tcaagcttgg attggataga agtcagttgg cacaggaagc 4860
caaatttgcc tcaggctctt cttctcggag tttttatgga ccactaggag cctgggataa 4920
ggatagtgga gaaatttacc ctgtagagac agacttgaaa ctagctatga ttatgttggt 4980
gctagaggac aagaaaaaac caatctctag ccgtgacggg atgaaacttt gtgtggaaac 5040
ctcgacgact tttgacgact gggttcgtca gtctgagaag gactatcagg atatgctgat 5100
ttatctcaag gaaaatgatt ttgccaagat tggagaatta acggagaaaa atgctctggc 5160
tatgcatgct acgacaaaga ctgctagtcc agccttttct tatctgacgg atgcctctta 5220
tgaggctatg gcctttgttc gccagcttcg tgagaaagga gaggcctgct actttaccat 5280
ggatgctggt cccaatgtta aggtcttctg tcaggagaaa gacttggagc atttgtcaga 5340
aattttcggt cagcgttatc gcttgattgt gtcaaaaaca aaggatttga gtcaagatga 5400
ttgctgttaa aacttgcgga aaactctatt gggcaggtga atatgctatt ttagagccag 5460
ggcagttagc tttgataaag gatattccca tctatatgag ggctgagatt gctttttctg 5520
acagctaccg tatctattca gatatgtttg atttcgcagt ggacttaagg cccaatcctg 5580
actacagctt gattcaagaa acgattgctt tgatgggaga cttcctcgct gttcgcggtc 5640
agaatttaag acctttttcc ctaaaaatct gtggcaaaat ggaacgagaa gggaaaaagt 5700
ttggtctagg ttctagtggc agcgtcgttg tcttggttgt caaggcttta ctggctctct 5760
ataatctttc ggttgatcag aatctcttgt tcaagctgac tagcgctgtc ttgctcaagc 5820
gaggagacaa tggttccatg ggcgaccttg cctgtattgt ggcagaggat ttggttcttt 5880
accagtcatt tgatcgccag aaggcggctg cttggttaga agaagaaaac ttggcgacag 5940
ttctggagcg tgattgggga ttttttatct cacaagtgaa accaacttta gaatgtgatt 6000
tcttagtggg atggaccaag gaagtggctg tatcgagtca catggtccag caaatcaagc 6060
aaaatatcaa tcaaaatttt ttaagttcct caaaagaaac ggtggtttct ttggtcgaag 6120
ccttggagca ggggaaagcc gaaaaagtta tcgagcaagt agaagtagcc agcaagcttt 6180
tagaaggctt gagtacagat atttacacgc ctttgcttag acagttgaaa gaagccagtc 6240
aagatttgca ggccgttgcc aagagtagtg gtgctggtgg tggtgactgt ggcatcgccc 6300
tgagttttga tgcgcaatct tctcgaaaca ctttaaaaaa tcgttgggcc gatctgggga 6360
ttgagctctt atatcaagaa aggataggac atgacgacaa atcgtaatct agccccggga 6420
ggagagaaat tatgcaaacg gaacacgtca ttttattgaa tgcacaggga gttcccacgg 6480
gtacgctgga aaagtatgcc gcacacacgg cagacacccg cttacatctc gcgttctcca 6540
gttggctgtt taatgccaaa ggacaattat tagttacccg ccgcgcactg agcaaaaaag 6600
catggcctgg cgtgtggact aactcggttt gtgggcaccc acaactggga gaaagcaacg 6660
aagacgcagt gatccgccgt tgccgttatg agcttggcgt ggaaattacg cctcctgaat 6720
ctatctatcc tgactttcgc taccgcgcca ccgatccgag tggcattgtg gaaaatgaag 6780
tgtgtccggt atttgccgca cgcaccacta gtgcgttaca gatcaatgat gatgaagtga 6840
tggattatca atggtgtgat ttagcagatg tattacacgg tattgatgcc acgccgtggg 6900
cgttcagtcc gtggatggtg atgcaggcga caaatcgcga agccagaaaa cgattatctg 6960
catttaccca gcttaaataa gcatgcacta gagtcgagga aacagaccat ggagtctcga 7020
gtgtggaatt gtgagcggat aacaatttca cacaggaaac agaccatgga attcatgagt 7080
tttgatattg ccaaataccc gaccctggca ctggtcgact ccacccagga gttacgactg 7140
ttgccgaaag agagtttacc gaaactctgc gacgaactgc gccgctattt actcgacagc 7200
gtgagccgtt ccagcgggca cttcgcctcc gggctgggca cggtcgaact gaccgtggcg 7260
ctgcactatg tctacaacac cccgtttgac caattgattt gggatgtggg gcatcaggct 7320
tatccgcata aaattttgac cggacgccgc gacaaaatcg gcaccatccg tcagaaaggc 7380
ggtctgcacc cgttcccgtg gcgcggcgaa agcgaatatg acgtattaag cgtcgggcat 7440
tcatcaacct ccatcagtgc cggaattggt attgcggttg ctgccgaaaa agaaggcaaa 7500
aatcgccgca ccgtctgtgt cattggcgat ggcgcgatta ccgcaggcat ggcgtttgaa 7560
gcgatgaatc acgcgggcga tatccgtcct gatatgctgg tgattctcaa cgacaatgaa 7620
atgtcgattt ccgaaaatgt cggcgcgctc aacaaccatc tggcacagct gctttccggt 7680
aagctttact cttcactgcg cgaaggcggg aaaaaagttt tctctggcgt gccgccaatt 7740
aaagagctgc tcaaacgcac cgaagaacat attaaaggca tggtagtgcc tggcacgttg 7800
tttgaagagc tgggctttaa ctacatcggc ccggtggacg gtcacgatgt gctggggctt 7860
atcaccacgc taaagaacat gcgcgacctg aaaggcccgc agttcctgca tatcatgacc 7920
aaaaaaggtc gtggttatga accggcagaa aaagacccga tcactttcca cgccgtgcct 7980
aaatttgatc cctccagcgg ttgtttgccg aaaagtagcg gcggtttgcc gagctattca 8040
aaaatctttg gcgactggtt gtgcgaaacg gcagcgaaag acaacaagct gatggcgatt 8100
actccggcga tgcgtgaagg ttccggcatg gtcgagtttt cacgtaaatt cccggatcgc 8160
tacttcgacg tggcaattgc cgagcaacac gcggtgacct ttgctgcggg tctggcgatt 8220
ggtgggtaca aacccattgt cgcgatttac tccactttcc tgcaacgcgc ctatgatcag 8280
gtgctgcatg acgtggcgat tcaaaagctt ccggtcctgt tcgccatcga ccgcgcgggc 8340
attgttggtg ctgacggtca aacccatcag ggtgcttttg atctctctta cctgcgctgc 8400
ataccggaaa tggtcattat gaccccgagc gatgaaaacg aatgtcgcca gatgctctat 8460
accggctatc actataacga tggcccgtca gcggtgcgct acccgcgtgg caacgcggtc 8520
ggcgtggaac tgacgccgct ggaaaaacta ccaattggca aaggcattgt gaagcgtcgt 8580
ggcgagaaac tggcgatcct taactttggt acgctgatgc cagaagcggc gaaagtcgcc 8640
gaatcgctga acgccacgct ggtcgatatg cgttttgtga aaccgcttga tgaagcgtta 8700
attctggaaa tggccgccag ccatgaagcg ctggtcaccg tagaagaaaa cgccattatg 8760
ggcggcgcag gcagcggcgt gaacgaagtg ctgatggccc atcgtaaacc agtacccgtg 8820
ctgaacattg gcctgccgga cttctttatt ccgcaaggaa ctcaggaaga aatgcgcgcc 8880
gaactcggcc tcgatgccgc tggtatggaa gccaaaatca aggcctggct ggcataaa 8938
<210> 95
<211> 14034
<212> DNA
<213> Artificial Sequence
<220>
<223> pT-HBSREYbbOdxrfree
<400> 95
ctagcacaga atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac 60
tcagaagtga aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg 120
aactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 180
ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa 240
cgttgcgaag caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca 300
tcaaattaag cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactctttt 360
tgtttatttt tctaaataca ttcaaatatg tatccgctca aagcttgcgt cagaccccgt 420
agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 480
aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 540
ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta 600
gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 660
aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 720
aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 780
gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 840
aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 900
aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 960
cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 1020
cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 1080
tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt 1140
tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga 1200
ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca 1260
ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac 1320
actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct 1380
gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc 1440
tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagcag 1500
atcaattcgc gcgcgaaggc gaagcggcat gcatttacgt tgacaccatc gaatggtgca 1560
aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg gtggtgaatg 1620
tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt 1680
cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg 1740
cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt 1800
cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg 1860
cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac 1920
gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg 1980
ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca 2040
ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt 2100
tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc 2160
aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct 2220
ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga 2280
gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg 2340
cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg 2400
ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat 2460
gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg 2520
tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg 2580
tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg 2640
cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt 2700
gagcgcaacg caattaatgt gagttagcgc gaattgatct ggtttgacag cttatcatcg 2760
actgcacggt gcaccaatgc ttctggcgtc aggcagccat cggaagctgt ggtatggctg 2820
tgcaggtcgt aaatcactgc ataattcgtg tcgctcaagg cgcactcccg ttctggataa 2880
tgttttttgc gccgacatca taacggttct ggcaaatatt ctgaaatgag ctgttgacaa 2940
ttaatcatcc ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt cacacaggaa 3000
acagaccatg gaattctacg tactagtgaa ttcaggaggt aataaatatg ggtctgatgc 3060
tgattgattg gtgtgcactg gctctggttg ttttcattgg cctgccgcac ggcgcgctgg 3120
atgctgccat ttctttttct atgatctctt ctgcaaaacg cattgctcgt ctggctggta 3180
ttctgctgat ctatctgctg ctggcgaccg cgttcttcct gatctggtat cagctgccag 3240
cgtttagcct gctgatcttc ctgctgatct ccattatcca ctttggtatg gcagacttca 3300
acgcgtcccc aagcaaactg aaatggccgc atatcatcgc ccacggcggt gttgttactg 3360
tttggctgcc gctgatccag aaaaacgaag taactaaact gtttagcatc ctgactaacg 3420
gtccgactcc gatcctgtgg gacatcctgc tgattttctt cctgtgttgg tctattggcg 3480
tgtgtctgca cacgtacgaa accctgcgct ctaaacatta caacatcgcc tttgaactga 3540
tcggtctgat tttcctggcg tggtatgcgc cgcctctggt tacgtttgcc acttacttct 3600
gcttcattca ttcccgtcgc cacttctcct ttgtgtggaa gcagctgcaa cacatgtctt 3660
ccaaaaagat gatgattggc agcgcgatta tcctgtcctg tacctcttgg ctgatcggcg 3720
gtggtatcta tttcttcctg aactccaaaa tgatcgcctc tgaggctgcg ctgcagactg 3780
tgttcatcgg tctggcggca ctgaccgtgc cgcacatgat tctgatcgac ttcatcttcc 3840
gtccgcactc ttcccgtatc aaaatcaaaa actaattaat taaaggaggt aataatatga 3900
ctcataaagc aacggagatc ctgacaggta aagttatgca aaaatcggtc ttaattaccg 3960
gatgttccag tggaattggc ctggaaagcg cgctcgaatt aaaacgccag ggttttcatg 4020
tgctggcagg ttgccggaaa ccggatgatg ttgagcgcat gaacagcatg ggatttaccg 4080
gcgtgttgat cgatctggat tcaccagaaa gtgttgatcg cgcagccgac gaggtgatcg 4140
ccctgaccga taattgtctg tatgggatct ttaacaatgc cggattcggc atgtatggcc 4200
ccctttccac catcagccgt gcgcagatgg aacagcagtt ttccgccaac tttttcggcg 4260
cacaccagct caccatgcgc ctgttacccg cgatgttacc gcacggtgaa gggcgtattg 4320
tgatgacatc atcggtgatg ggattaatct ccacgccggg tcgtggcgct tacgcggcca 4380
gtaaatatgc gctggaggcg tggtcagatg cactgcgcat ggagctgcgc cacagcggaa 4440
ttaaagtcag cctgatcgaa cccggtccca ttcgtactcg cttcaccgac aacgtcaacc 4500
agacgcaaag tgataaacca gtcgaaaatc ccggcatcgc cgcccgcttt acgttgggac 4560
cggaagcggt ggtggacaaa gtacgccatg cttttattag cgagaagccg aagatgcgct 4620
atccggtgac gctggtgacc tgggcggtaa tggtgcttaa gcgcctgctg ccggggcgcg 4680
tgatggacaa aatattgcag gggtaactag taggaggtaa taaatatggt gagtggcagt 4740
aaagcgggcg tttcgcctca tcgcgaaata gaagtaatga gacaatccat tgacgatcac 4800
ctggctggcc tgttacctga aaccgacagc caggatatcg tcagccttgc gatgcgtgaa 4860
ggcgtcatgg cacccggtaa acggatccgt ccgctgctga tgctgctggc cgcccgcgac 4920
ctccgctacc agggcagtat gcctacgctg ctcgatctcg cctgcgccgt tgaactgacc 4980
cataccgcgt cgctgatgct cgacgacatg ccctgcatgg acaacgccga gctgcgccgc 5040
ggtcagccca ctacccacaa aaaatttggt gagagcgtgg cgatccttgc ctccgttggg 5100
ctgctctcta aagcctttgg tctgatcgcc gccaccggcg atctgccggg ggagaggcgt 5160
gcccaggcgg tcaacgagct ctctaccgcc gtgggcgtgc agggcctggt actggggcag 5220
tttcgcgatc ttaacgatgc cgccctcgac cgtacccctg acgctatcct cagcaccaac 5280
cacctcaaga ccggcattct gttcagcgcg atgctgcaga tcgtcgccat tgcttccgcc 5340
tcgtcgccga gcacgcgaga gacgctgcac gccttcgccc tcgacttcgg ccaggcgttt 5400
caactgctgg acgatctgcg tgacgatcac ccggaaaccg gtaaagatcg caataaggac 5460
gcgggaaaat cgacgctggt caaccggctg ggcgcagacg cggcccggca aaagctgcgc 5520
gagcatattg attccgccga caaacacctc acttttgcct gtccgcaggg cggcgccatc 5580
cgacagttta tgcatctgtg gtttggccat caccttgccg actggtcacc ggtcatgaaa 5640
atcgcctgat aactcgagga ggtataaagg atgaaaaaaa ccgttgtgat tggcgcaggc 5700
tttggtggcc tggcgctggc gattcgcctg caggcggcag ggatcccaac cgtactgctg 5760
gagcagcggg acaagcccgg cggtcgggcc tacgtctggc atgaccaggg ctttaccttt 5820
gacgccgggc cgacggtgat caccgatcct accgcgcttg aggcgctgtt caccctggcc 5880
ggcaggcgca tggaggatta cgtcaggctg ctgccggtaa aacccttcta ccgactctgc 5940
tgggagtccg ggaagaccct cgactatgct aacgacagcg ccgagcttga ggcgcagatt 6000
acccagttca acccccgcga cgtcgagggc taccggcgct ttctggctta ctcccaggcg 6060
gtattccagg agggatattt gcgcctcggc agcgtgccgt tcctctcttt tcgcgacatg 6120
ctgcgcgccg ggccgcagct gcttaagctc caggcgtggc agagcgtcta ccagtcggtt 6180
tcgcgcttta ttgaggatga gcatctgcgg caggccttct cgttccactc cctgctggta 6240
ggcggcaacc ccttcaccac ctcgtccatc tacaccctga tccacgccct tgagcgggag 6300
tggggggtct ggttccctga gggcggcacc ggggcgctgg tgaacggcat ggtgaagctg 6360
tttaccgatc tgggcgggga gatcgaactc aacgcccggg tcgaagagct ggtggtggcc 6420
gataaccgcg taagccaggt ccggctggcg gatggtcgga tctttgacac cgacgccgta 6480
gcctcgaacg ctgacgtggt gaacacctat aaaaagctgc tcggccacca tccggtgggg 6540
cagaagcggg cggcagcgct ggagcgcaag agcatgagca actcgctgtt tgtgctctac 6600
ttcggcctga accagcctca ttcccagctg gcgcaccata ccatctgttt tggtccccgc 6660
taccgggagc tgatcgacga gatctttacc ggcagcgcgc tggcggatga cttctcgctc 6720
tacctgcact cgccctgcgt gaccgatccc tcgctcgcgc ctcccggctg cgccagcttc 6780
tacgtgctgg ccccggtgcc gcatcttggc aacgcgccgc tggactgggc gcaggagggg 6840
ccgaagctgc gcgaccgcat ctttgactac cttgaagagc gctatatgcc cggcctgcgt 6900
agccagctgg tgacccagcg gatctttacc ccggcagact tccacgacac gctggatgcg 6960
catctgggat cggccttctc catcgagccg ctgctgaccc aaagcgcctg gttccgcccg 7020
cacaaccgcg acagcgacat tgccaacctc tacctggtgg gcgcaggtac tcaccctggg 7080
gcgggcattc ctggcgtagt ggcctcggcg aaagccaccg ccagcctgat gattgaggat 7140
ctgcaatgag ccaaccgccg ctgcttgacc acgccacgca gaccatggcc aacggctcga 7200
aaagttttgc caccgctgcg aagctgttcg acccggccac ccgccgtagc gtgctgatgc 7260
tctacacctg gtgccgccac tgcgatgacg tcattgacga ccagacccac ggcttcgcca 7320
gcgaggccgc ggcggaggag gaggccaccc agcgcctggc ccggctgcgc acgctgaccc 7380
tggcggcgtt tgaaggggcc gagatgcagg atccggcctt cgctgccttt caggaggtgg 7440
cgctgaccca cggtattacg ccccgcatgg cgctcgatca cctcgacggc tttgcgatgg 7500
acgtggctca gacccgctat gtcacctttg aggatacgct gcgctactgc tatcacgtgg 7560
cgggcgtggt gggtctgatg atggccaggg tgatgggcgt gcgggatgag cgggtgctgg 7620
atcgcgcctg cgatctgggg ctggccttcc agctgacgaa tatcgcccgg gatattattg 7680
acgatgcggc tattgaccgc tgctatctgc ccgccgagtg gctgcaggat gccgggctga 7740
ccccggagaa ctatgccgcg cgggagaatc gggccgcgct ggcgcgggtg gcggagcggc 7800
ttattgatgc cgcagagccg tactacatct cctcccaggc cgggctacac gatctgccgc 7860
cgcgctgcgc ctgggcgatc gccaccgccc gcagcgtcta ccgggagatc ggtattaagg 7920
taaaagcggc gggaggcagc gcctgggatc gccgccagca caccagcaaa ggtgaaaaaa 7980
ttgccatgct gatggcggca ccggggcagg ttattcgggc gaagacgacg agggtgacgc 8040
cgcgtccggc cggtctttgg cagcgtcccg tttagtaatc tagaggaggt aataaaatat 8100
gcttcgttcg ttgctcagag gcctcacgca tatcccccgc gtgaactccg cccagcagcc 8160
cagctgtgca cacgcgcgac tccagtttaa gctcaggagc atgcagatga cgctcatgca 8220
gcccagcatc tcagccaatc tgtcgcgcgc cgaggaccgc acagaccaca tgaggggtgc 8280
aagcacctgg gcaggcgggc agtcgcagga tgagctgatg ctgaaggacg agtgcatctt 8340
ggtggatgtt gaggacaaca tcacaggcca tgccagcaag ctggagtgtc acaagttcct 8400
accacatcag cctgcaggcc tgctgcaccg ggccttctct gtgttcctgt ttgacgatca 8460
ggggcgactg ctgctgcaac agcgtgcacg ctcaaaaatc accttcccaa gtgtgtggac 8520
gaacacctgc tgcagccacc ctttacatgg gcagacccca gatgaggtgg accaactaag 8580
ccaggtggcc gacggaacag tacctggcgc aaaggctgct gccatccgca agttggagca 8640
cgagctgggg ataccagcgc accagctgcc ggcaagcgcg tttcgcttcc tcacgcgttt 8700
gcactactgt gccgcggacg tgcagccagc tgcgacacaa tcagcgctct ggggcgagca 8760
cgaaatggac tacatcttgt tcatccgggc caacgtcacc ttggcgccca accctgacga 8820
ggtggacgaa gtcaggtacg tgacgcaaga ggagctgcgg cagatgatgc agccggacaa 8880
cgggctgcaa tggtcgccgt ggtttcgcat catcgccgcg cgcttccttg agcgttggtg 8940
ggctgacctg gacgcggccc taaacactga caaacacgag gattggggaa cggtgcatca 9000
catcaacgaa gcgtgataag cggccgcgct gttgacaatt aatcatccgg ctcgtataat 9060
gtgtggaatt gtgagcggat aacaatttca cacaggaaac agaccatgga gttgaaaaca 9120
gtagttatta ttgatgcatt acgaacacca attggaaaat ataaaggcag cttaagtcaa 9180
gtaagtgccg tagacttagg aacacatgtt acaacacaac ttttaaaaag acattccact 9240
atttctgaag aaattgatca agtaatcttt ggaaatgttt tacaagctgg aaatggccaa 9300
aatcccgcac gacaaatagc aataaacagc ggtttatctc atgaaattcc cgcaatgaca 9360
gttaatgagg tctgcggatc aggaatgaag gccgttattt tggcgaaaca attgattcaa 9420
ttaggagaag cggaagtttt aattgctggc gggattgaga atatgtccca agcacctaaa 9480
ttacaacgat ttaattacga aacagaaagc tatgatgcgc ctttttctag tatgatgtac 9540
gatgggttaa cggatgcctt tagtggtcaa gcaatgggct taactgctga aaatgtggcc 9600
gaaaagtatc atgtaactag agaagagcaa gatcaatttt ctgtacattc acaattaaaa 9660
gcagctcaag cacaagcaga agggatattc gctgacgaaa tagccccatt agaagtatca 9720
ggaacgcttg tggagaaaga tgaagggatt cgccctaatt cgagcgttga gaagctagga 9780
acgcttaaaa cagtttttaa agaagacggt actgtaacag cagggaatgc atcaaccatt 9840
aatgatgggg cttctgcttt gattattgct tcacaagaat atgccgaagc acacggtctt 9900
ccttatttag ctattattcg agacagtgtg gaagtcggta ttgatccagc ctatatggga 9960
atttcgccga ttaaagccat tcaaaaactg ttagcgcgca atcaacttac tacggaagaa 10020
attgatctgt atgaaatcaa cgaagcattt gcagcaactt caatcgtggt ccaaagagaa 10080
ctggctttac cagaggaaaa ggtcaacatt tatggtggcg gtatttcatt aggtcatgcg 10140
attggtgcca caggtgctcg tttattaacg agtttaagtt atcaattaaa tcaaaaagaa 10200
aagaaatatg gagtggcttc tttatgtatc ggcggtggct taggactcgc tatgctacta 10260
gagagacctc agcaaaaaaa aaacagccga ttttatcaaa tgagtcctga ggaacgcctg 10320
gcttctcttc ttaatgaagg ccagatttct gctgatacaa aaaaagaatt tgaaaatacg 10380
gctttatctt cgcagattgc caatcatatg attgaaaatc aaatcagtga aacagaagtg 10440
ccgatgggcg ttggcttaca tttaacagtg gacgaaactg attatttggt accaatggcg 10500
acagaagagc cctcagtgat tgcggctttg agtaatggtg caaaaatagc acaaggattt 10560
aaaacagtga atcaacaacg tttaatgcgt ggacaaatcg ttttttacga tgttgcagac 10620
gccgagtcat tgattgatga actacaagta agagaaacgg aaatttttca acaagcagag 10680
ttaagttatc catctatcgt taaacgcggc ggcggcttaa gagatttgca atatcgtgct 10740
tttgatgaat catttgtatc tgtcgacttt ttagtagatg ttaaggatgc aatgggggca 10800
aatatcgtta acgctatgtt ggaaggtgtg gccgagttgt tccgtgaatg gtttgcggag 10860
caaaagattt tattcagtat tttaagtaat tatgccacgg agtcggttgt tacgatgaaa 10920
acggctattc cagtttcacg tttaagtaag gggagcaatg gccgggaaat tgctgaaaaa 10980
attgttttag cttcacgcta tgcttcatta gatccttatc gggcagtcac gcataacaaa 11040
gggatcatga atggcattga agctgtcgtt ttagctacag gaaatgatac acgcgctgtt 11100
agcgcttctt gtcatgcttt tgcggtgaag gaaggtcgct accaaggttt gactagttgg 11160
acgctggatg gcgaacaact aattggtgaa atttcagttc cgcttgcgtt agccacggtt 11220
ggcggtgcca caaaagtctt acctaaatct caagcagctg ctgatttgtt agcagtgacg 11280
gatgcaaaag aactaagtcg agtagtagcg gctgttggtt tggcacaaaa tttagcggcg 11340
ttacgggcct tagtctctga aggaattcaa aaaggacaca tggctctaca agcacgttct 11400
ttagcgatga cggtcggagc tactggtaaa gaagttgagg cagtcgctca acaattaaaa 11460
cgtcaaaaaa cgatgaacca agaccgagcc ttggctattt taaatgattt aagaaaacaa 11520
taaaaaaaca gttcagcaga aattattctg ctgaactgtt ttttttcaca ttaggtagcc 11580
gtttcagctc gacaggagga gcggctatgc aaccgcatta tgatctgatt ctcgtggggg 11640
ctggactcgc gaatggcctt atcgccctgc gtcttcagca gcagcaacct gatatgcgta 11700
ttttgcttat cgacgccgca ccccaggcgg gcgggaatca tacgtggtca tttcaccacg 11760
atgatttgac tgagagccaa catcgttgga tagctccgct ggtggttcat cactggcccg 11820
actatcaggt acgctttccc acacgccgtc gtaagctgaa cagcggctac ttttgtatta 11880
cttctcagcg tttcgctgag gttttacagc gacagtttgg cccgcacttg tggatggata 11940
ccgcggtcgc agaggttaat gcggaatctg ttcggttgaa aaagggtcag gttatcggtg 12000
cccgcgcggt gattgacggg cggggttatg cggcaaattc agcactgagc gtgggcttcc 12060
aggcgtttat tggccaggaa tggcgattga gccacccgca tggtttatcg tctcccatta 12120
tcatggatgc cacggtcgat cagcaaaatg gttatcgctt cgtgtacagc ctgccgctct 12180
cgccgaccag attgttaatt gaagacacgc actatattga taatgcgaca ttagatcctg 12240
aatgcgcgcg gcaaaatatt tgcgactatg ccgcgcaaca gggttggcag cttcagacac 12300
tgctgcgaga agaacagggc gccttaccca ttactctgtc gggcaatgcc gacgcattct 12360
ggcagcagcg ccccctggcc tgtagtggat tacgtgccgg tctgttccat cctaccaccg 12420
gctattcact gccgctggcg gttgccgtgg ccgaccgcct gagtgcactt gatgtcttta 12480
cgtcggcctc aattcaccat gccattacgc attttgcccg cgagcgctgg cagcagcagg 12540
gctttttccg catgctgaat cgcatgctgt ttttagccgg acccgccgat tcacgctggc 12600
gggttatgca gcgtttttat ggtttacctg aagatttaat tgcccgtttt tatgcgggaa 12660
aactcacgct gaccgatcgg ctacgtattc tgagcggcaa gccgcctgtt ccggtattag 12720
cagcattgca agccattatg acgactcatc gttaactgca ggcatgagta ctgtgtggaa 12780
ttgtgagcgg ataacaattt cacacaggaa acagaccatg gaattcgagc tcatgaagca 12840
actcaccatt ctgggctcga ccggctcgat tggttgcagc acgctggacg tggtgcgcca 12900
taatcccgaa cacttccgcg tagttgcgct ggtggcaggc aaaaatgtca ctcgcatggt 12960
agaacagtgc ctggaattct ctccccgcta tgccgtaatg gacgatgaag cgagtgcgaa 13020
acttcttaaa acgatgctac agcaacaggg tagccgcacc gaagtcttaa gtgggcaaca 13080
agccgcttgc gatatggcag cgcttgagga tgttgatcag gtgatggcag ccattgttgg 13140
cgctgctggg ctgttaccta cgcttgctgc gatccgcgcg ggtaaaacca ttttgctggc 13200
caataaagaa tcactggtta cctgcggacg tctgtttatg gacgccgtaa agcagagcaa 13260
agcgcaattg ttaccggtcg atagcgaaca taacgccatt tttcagagtt taccgcaacc 13320
tatccagcat aatctgggat acgctgacct tgagcaaaat ggcgtggtgt ccattttact 13380
taccgggtct ggtggccctt tccgtgagac gccattgcgc gatttggcaa caatgacgcc 13440
ggatcaagcc tgccgtcatc cgaactggtc gatggggcgt aaaatttctg tcgattcggc 13500
taccatgatg aacaaaggtc tggaatacat tgaagcgcgt tggctgttta acgccagcgc 13560
cagccagatg gaagtgctga ttcacccgca gtcagtgatt cactcaatgg tgcgctatca 13620
ggacggcagt gttctggcgc agctggggga accggatatg cgtacgccaa ttgcccacac 13680
catggcatgg ccgaatcgcg tgaactctgg cgtgaagccg ctcgattttt gcaaactaag 13740
tgcgttgaca tttgccgcac cggattatga tcgttatcca tgcctgaaac tggcgatgga 13800
ggcgttcgaa caaggccagg cagcgacgac agcattgaat gccgcaaacg aaatcaccgt 13860
tgctgctttt cttgcgcaac aaatccgctt tacggatatc gctgcgttga atttatccgt 13920
actggaaaaa atggatatgc gcgaaccaca atgtgtggac gatgtgttat ctgttgatgc 13980
gaacgcgcgt gaagtcgcca gaaaagaggt gatgcgtctc gcaagctgat ctag 14034
<210> 96
<211> 3355
<212> DNA
<213> Artificial Sequence
<220>
<223> pEGFP
<400> 96
agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 60
acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 120
tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 180
ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg 240
catgcctgca ggtcgactct agaggatccc cgggtaccgg tcgccaccat ggtgagcaag 300
ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac 360
ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc 420
ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc 480
ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc 540
ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac 600
ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc 660
gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac 720
aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg 780
aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag 840
cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc 900
cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc 960
gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccgcgac 1020
tctagaattc caactgagcg ccggtcgcta ccattaccaa cttgtctggt gtcaaaaata 1080
ataggcctac tagtcggccg tacgggccct ttcgtctcgc gcgtttcggt gatgacggtg 1140
aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg 1200
ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta 1260
actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc 1320
acagatgcgt aaggagaaaa taccgcatca ggcggcctta agggcctcgt gatacgccta 1380
tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg 1440
ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 1500
ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 1560
attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 1620
gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 1680
ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 1740
cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 1800
gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 1860
tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 1920
gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 1980
ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 2040
tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 2100
gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 2160
caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 2220
cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 2280
atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 2340
gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 2400
attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 2460
cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 2520
atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 2580
tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 2640
ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 2700
ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac 2760
cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 2820
gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 2880
gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 2940
acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 3000
gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 3060
agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 3120
tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 3180
agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 3240
cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 3300
gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaag 3355
<210> 97
<211> 4616
<212> DNA
<213> Artificial Sequence
<220>
<223> pEGFP-dxr
<400> 97
ctagtcggcc gtacgggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct 60
gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac 120
aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg 180
catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg 240
taaggagaaa ataccgcatc aggcggcctt aagggcctcg tgatacgcct atttttatag 300
gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg 360
cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga 420
caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat 480
ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca 540
gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc 600
gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca 660
atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg 720
caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca 780
gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata 840
accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag 900
ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg 960
gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca 1020
acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta 1080
atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct 1140
ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca 1200
gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag 1260
gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat 1320
tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt 1380
taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 1440
cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 1500
gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 1560
gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 1620
agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 1680
aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 1740
agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 1800
cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 1860
accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 1920
aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 1980
ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 2040
cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 2100
gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 2160
tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 2220
agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc 2280
aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc 2340
gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca 2400
ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa 2460
caatttcaca caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc 2520
gactctagag gatccccggg taccggtcgc caccatggtg agcaagggcg aggagctgtt 2580
caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag 2640
cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg 2700
caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt 2760
gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat 2820
gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac 2880
ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat 2940
cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca 3000
caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg 3060
ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat 3120
cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag 3180
caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg 3240
gatcactctc ggcatggacg agctgtacaa gtaaagcggc cgcgactcta gaattccaac 3300
tgagcgccgg tcgctaccat taccaacttg tctggtgtca aaaataatag gactgtgtgg 3360
aattgtgagc ggataacaat ttcacacagg aaacagacca tggaattcga gctcatgaag 3420
caactcacca ttctgggctc gaccggctcg attggttgca gcacgctgga cgtggtgcgc 3480
cataatcccg aacacttccg cgtagttgcg ctggtggcag gcaaaaatgt cactcgcatg 3540
gtagaacagt gcctggaatt ctctccccgc tatgccgtaa tggacgatga agcgagtgcg 3600
aaacttctta aaacgatgct acagcaacag ggtagccgca ccgaagtctt aagtgggcaa 3660
caagccgctt gcgatatggc agcgcttgag gatgttgatc aggtgatggc agccattgtt 3720
ggcgctgctg ggctgttacc tacgcttgct gcgatccgcg cgggtaaaac cattttgctg 3780
gccaataaag aatcactggt tacctgcgga cgtctgttta tggacgccgt aaagcagagc 3840
aaagcgcaat tgttaccggt cgatagcgaa cataacgcca tttttcagag tttaccgcaa 3900
cctatccagc ataatctggg atacgctgac cttgagcaaa atggcgtggt gtccatttta 3960
cttaccgggt ctggtggccc tttccgtgag acgccattgc gcgatttggc aacaatgacg 4020
ccggatcaag cctgccgtca tccgaactgg tcgatggggc gtaaaatttc tgtcgattcg 4080
gctaccatga tgaacaaagg tctggaatac attgaagcgc gttggctgtt taacgccagc 4140
gccagccaga tggaagtgct gattcacccg cagtcagtga ttcactcaat ggtgcgctat 4200
caggacggca gtgttctggc gcagctgggg gaaccggata tgcgtacgcc aattgcccac 4260
accatggcat ggccgaatcg cgtgaactct ggcgtgaagc cgctcgattt ttgcaaacta 4320
agtgcgttga catttgccgc accggattat gatcgttatc catgcctgaa actggcgatg 4380
gaggcgttcg aacaaggcca ggcagcgacg acagcattga atgccgcaaa cgaaatcacc 4440
gttgctgctt ttcttgcgca acaaatccgc tttacggata tcgctgcgtt gaatttatcc 4500
gtactggaaa aaatggatat gcgcgaacca caatgtgtgg acgatgtgtt atctgttgat 4560
gcgaacgcgc gtgaagtcgc cagaaaagag gtgatgcgtc tcgcaagctg atctag 4616
<210> 98
<211> 2230
<212> DNA
<213> Artificial Sequence
<220>
<223> pORI19
<400> 98
ggaggctcaa gggagtatga gggaatgaaa ttccctcatg ggtttgattt taaaaattgc 60
ttgcaatttt gccgagcggt agcgctggaa aatttttgaa aaaaatttgg aatttggaaa 120
aaaatggggg gaaaggaagc gaattttgct tccgtactac gaccccccat taagtgccga 180
gtgccaattt ttgtgccaaa aacgctctat cccaactggc tcaagggttt aaggggtttt 240
tcaatcgcca acgaatcgcc aacgttttcg ccaacgtttt ttataaatct atatttaagt 300
agctttattg ttgtttttat gattacaaag tgatacacta actttataaa attatttgat 360
tggagttttt taaatggtga tttcagaatc gctagttcta gtgcaccata tgcggtgtga 420
aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct 480
gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 540
agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 600
ttgtaaaacg acggccagtg aattcgagct cggtacccgg ggatcctcta gagtcgacct 660
gcaggcatgc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 720
cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 780
aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 840
acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 900
ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 960
gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 1020
caggaaagaa catggatctc gacccgtgct ataattatac taattttata aggaggaaaa 1080
aatatgggca tttttagtat ttttgtaatc agcacagttc attatcaacc aaacaaaaaa 1140
taagtggtta taatgaatcg ttaataagca aaattcatat aaccaaatta aagagggtta 1200
taatgaacga gaaaaatata aaacacagtc aaaactttat tacttcaaaa cataatatag 1260
ataaaataat gacaaatata agattaaatg aacatgataa tatctttgaa atcggctcag 1320
gaaaaggcca ttttaccctt gaattagtaa agaggtgtaa tttcgtaact gccattgaaa 1380
tagaccataa attatgcaaa actacagaaa ataaacttgt tgatcacgat aatttccaag 1440
ttttaaacaa ggatatattg cagtttaaat ttcctaaaaa ccaatcctat aaaatatatg 1500
gtaatatacc ttataacata agtacggata taatacgcaa aattgttttt gatagtatag 1560
ctaatgagat ttatttaatc gtggaatacg ggtttgctaa aagattatta aatacaaaac 1620
gctcattggc attactttta atggcagaag ttgatatttc tatattaagt atggttccaa 1680
gagaatattt tcatcctaaa cctaaagtga atagctcact tatcagatta agtagaaaaa 1740
aatcaagaat atcacacaaa gataaacaaa agtataatta tttcgttatg aaatgggtta 1800
acaaagaata caagaaaata tttacaaaaa atcaatttaa caattcctta aaacatgcag 1860
gaattgacga tttaaacaat attagctttg aacaattctt atctcttttc aatagctata 1920
aattatttaa taagtaagtt aagggatgca taaactgcat cccttaactt gtttttcgtg 1980
tgcctatttt ttgtgaatcg ggtcgaggcc tcggactagc gattttttat taaaacgtct 2040
caaaatcgtt tctgagacgt tttagcgttt atttcgttta gttatcggca taatcgttaa 2100
aacaggcgtt atcgtagcgt aaaagccctt gagcgtagcg tggctttgca gcgaagatgt 2160
tgtctgttag attatgaaag ccgatgactg aatgaaataa taagcgcagc gcccttctat 2220
ttcggttgga 2230
<210> 99
<211> 4215
<212> DNA
<213> Artificial Sequence
<220>
<223> pORI19-mvaA
<400> 99
ggaggctcaa gggagtatga gggaatgaaa ttccctcatg ggtttgattt taaaaattgc 60
ttgcaatttt gccgagcggt agcgctggaa aatttttgaa aaaaatttgg aatttggaaa 120
aaaatggggg gaaaggaagc gaattttgct tccgtactac gaccccccat taagtgccga 180
gtgccaattt ttgtgccaaa aacgctctat cccaactggc tcaagggttt aaggggtttt 240
tcaatcgcca acgaatcgcc aacgttttcg ccaacgtttt ttataaatct atatttaagt 300
agctttattg ttgtttttat gattacaaag tgatacacta actttataaa attatttgat 360
tggagttttt taaatggtga tttcagaatc gctagttcta gtgcaccata tgcggtgtga 420
aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct 480
gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 540
agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 600
ttgtaaaacg acggccagtg aattcgagct cggtaccagc ttcaaccatt aatgaagttt 660
gtggttcagg gatgaaggca gtaattctag ctaagcaact tttacagttg ggcgaagcag 720
aagttgttat tgctggtgga acggaatcaa tgtctaatgc gcctattctt aaaaatcgta 780
aaactgaaga agaaacatta agcatgttaa gtgacggctt aattgatgct ttttctggca 840
tttcaatggg aattattgga gaaacaattg cggagcagtt tgatgttagt cgtgctgacc 900
aagacctatt cgcacaaaat tctcaagaga aggcagtagc agccagtcaa gcaggaattt 960
ttaatgacga gatcgtagct cttgggaaac ttactgttga tgaaactcct cgaccttcat 1020
ctagcctaga aaaattggct acattacgga cagcctttaa ggaaaatgga acagttacag 1080
ctgggaattc ttcaccagtt aatgacggag cttctgcttt aattttggcc actaaagagt 1140
atgctgaaaa acatggaatt tcttatttag ctgaattagt tgagagtgcg gaagttggta 1200
ttgaccctgc aattatggga gtatcaccga ttgatgcaat tcaaaaatta gttcaacgct 1260
caggcgttca gctttcagaa atagatcttt ttgaaattaa tgaggccttt gctgcatcaa 1320
gtattgctgt taatcgtgaa ttgggcttaa aagattctca agttaatatt tatggaggag 1380
cgattgctct tggacatgct attgggtcaa gtggtgccaa aatcctgaca actttgggat 1440
atgctttgaa acgagaacaa aagcgttatg gaattgctag tctttgtatt ggcggtggtc 1500
tagggcttgc tattttattg aaaaatcctg acttttaaaa attgatggag gaattgccca 1560
gtatttctgg gcatttttgg gaaatatgag aaaaaaattt taactaaaaa atttgagaaa 1620
ataatagcct gtcaagattt tttcttgaca gcatgaaatt agtctgctat aatattaaat 1680
ataataaaaa agaaatagga aatataaaat gtctgtaaaa attcgtttga ctcgtatggg 1740
ttctaagaaa aaacctttct accgtattaa tgttgctgat tcacgtgcac cacgtgatgg 1800
taaattcatc gaaacagttg gaacttacaa tccacttgta actgaaaacc aagtaactct 1860
taaagaagaa cgcgtattgg aatggttgag caacggagca caaccttcag atacagttcg 1920
taacctcctt tcaaaagcgg gcgttatgaa aaaattccac gaatcaaaac tttctaaata 1980
atttttgaga taagctgctt tttagcagct tttcgtgaat ataaaaatca cataaaatgg 2040
acttgaataa ctttcaagtt agagtagagg taagaaatgc aaaaagatgt gaaagaactc 2100
gttttaacta ttgtaaaacc tttggtgact cagcctgatg aagtttcttt ggaaattatt 2160
gaaggtgagg agtttatgga ataccatctc aaagttgctg acggagatat tggaagaata 2220
attggacgtc aaggtcgaat tattcaagcc attcgtacag ttgtatactt tgttcctgtt 2280
gaagggaaaa aagttcgact cctcgtggac caataataag taaaaataaa agaactcatc 2340
attgatgagt tcttttattt ttggaaaacg gaacgttctt ttgagaaata aaatagttat 2400
ttagaatttc cttttattgt ccccaaaaat atgctagaat gaaaaaatat tgattaataa 2460
ttgtagaaat aatcagtgat aaatataaat taactgattt ttatataggt ggataatgga 2520
taagaaaaaa ggaatcttat tagtagcttt agggacgcct cgttcttgtg agacagagga 2580
tgtaagggaa tatttaaaag aatttttggg tgatccactc gtcattcatc ctctagagtc 2640
gacctgcagg catgcaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 2700
ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 2760
tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 2820
gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 2880
gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 2940
gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 3000
taacgcagga aagaacatgg atctcgaccc gtgctataat tatactaatt ttataaggag 3060
gaaaaaatat gggcattttt agtatttttg taatcagcac agttcattat caaccaaaca 3120
aaaaataagt ggttataatg aatcgttaat aagcaaaatt catataacca aattaaagag 3180
ggttataatg aacgagaaaa atataaaaca cagtcaaaac tttattactt caaaacataa 3240
tatagataaa ataatgacaa atataagatt aaatgaacat gataatatct ttgaaatcgg 3300
ctcaggaaaa ggccatttta cccttgaatt agtaaagagg tgtaatttcg taactgccat 3360
tgaaatagac cataaattat gcaaaactac agaaaataaa cttgttgatc acgataattt 3420
ccaagtttta aacaaggata tattgcagtt taaatttcct aaaaaccaat cctataaaat 3480
atatggtaat ataccttata acataagtac ggatataata cgcaaaattg tttttgatag 3540
tatagctaat gagatttatt taatcgtgga atacgggttt gctaaaagat tattaaatac 3600
aaaacgctca ttggcattac ttttaatggc agaagttgat atttctatat taagtatggt 3660
tccaagagaa tattttcatc ctaaacctaa agtgaatagc tcacttatca gattaagtag 3720
aaaaaaatca agaatatcac acaaagataa acaaaagtat aattatttcg ttatgaaatg 3780
ggttaacaaa gaatacaaga aaatatttac aaaaaatcaa tttaacaatt ccttaaaaca 3840
tgcaggaatt gacgatttaa acaatattag ctttgaacaa ttcttatctc ttttcaatag 3900
ctataaatta tttaataagt aagttaaggg atgcataaac tgcatccctt aacttgtttt 3960
tcgtgtgcct attttttgtg aatcgggtcg aggcctcgga ctagcgattt tttattaaaa 4020
cgtctcaaaa tcgtttctga gacgttttag cgtttatttc gtttagttat cggcataatc 4080
gttaaaacag gcgttatcgt agcgtaaaag cccttgagcg tagcgtggct ttgcagcgaa 4140
gatgttgtct gttagattat gaaagccgat gactgaatga aataataagc gcagcgccct 4200
tctatttcgg ttgga 4215
<210> 100
<211> 5790
<212> DNA
<213> Artificial Sequence
<220>
<223> pCI372
<400> 100
gtaaatcgct cctttttagg tggcacaaat gtgaggcatt ttcgctcttt ccggcgaggc 60
tagttaccct taagttattg gtatgactgg ttttaagcgc aaaaaaagtt gctttttcgt 120
acctattaat gtatcgttag aaaaccgact gtaaaaagta cagtcggcat tatctcatat 180
tataaaagcc agtcattagg cctatctgac aattcctgaa tagagttcat aaacaatcct 240
gcatgataac catcacaaac agaatgatgt acctgtaaag atagcggtaa atatattgaa 300
ttacctttat taatgaattt tcctgctgta ataatgggta gaaggtaatt actattatta 360
ttgatattta agttaaaccc agtaaatgaa gtccatggaa taatagaaag agaaaaagca 420
ttttcaggta taggtgtttt gggaaacaat ttccccgaac cattatattt ctctacatca 480
gaaaggtata aatcataaaa ctctttgaag tcattcttta caggagtcca aataccagag 540
aatgttttag atacaccatc aaaaattgta taaagtggct ctaacttatc ccaataacct 600
aactctccgt cgctattgta accagttcta aaagctgtat ttgagtttat cacccttgtc 660
actaagaaaa taaatgcagg gtaaaattta tatccttctt gttttatgtt tcggtataaa 720
acactaatat caatttctgt ggttatacta aaagtcgttt gttggttcaa ataatgatta 780
aatatctctt ttctcttcca attgtctaaa tcaattttat taaagttcat ttgatatgcc 840
tcctaaattt ttatcttgct cttttgtcag agaaatcata actctttttt tcgattctga 900
aatcaccatt taaaaaactc caatcaaata attttataaa attagtgtat cactttgtaa 960
tcataaaaac aacaataaag ctacttaaat atagatttat aaaaaacgtt ggcgaaaacg 1020
ttggcgattc gttggcgatt gaaaaacccc tcaaaccctt gagccagttg ggatagagcg 1080
tttttggcac aaaaattggc actcggcact taatgggggg tcgtagtacg gaagcaaaat 1140
tcgcttcctt tccccccatt tttttccaaa ttccaaattt ttttcaaaaa ttttccagcg 1200
ctaccgctcg gcaaaattgc aagcaatttt taaaatcaaa cccatgaggg aatttcattc 1260
cctcaaactc ccttgagcct cctccaaccg aaatagaagg acgctgcgct tattatttca 1320
ttcagtcatc ggctttcata atctaacaga caacatcttc gctgcaaagc cacgctacgc 1380
tcaagggctt ttacgctacg ataacgcctg ttttaacgat tatgccgata actaaacgaa 1440
ataaacgcta aaacgtctca gaaacgattt tgagacgttt taataaaaaa tcgacttcgt 1500
tcttttttta cctctcggtt atgagttagt tcaaattcgt tctttttagg ttctaaatcg 1560
tgtttttctt ggaattgtgc tgttttatcc tttaccttgt ctacaaaccc cttaaaaacg 1620
tttttaaagg cttttaagcc gtctgtacgt tccttaagga attcgagctc ggtacccggg 1680
gatcctctag agtcgacctg cagcaatggc aacaacgttg cgcaaactat taactggcga 1740
actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc 1800
aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 1860
cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg 1920
tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat 1980
cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata 2040
tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 2100
ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 2160
ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 2220
cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 2280
aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct 2340
agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 2400
tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 2460
ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 2520
cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 2580
atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 2640
ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 2700
tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 2760
gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 2820
gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac 2880
cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt 2940
gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat 3000
ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca 3060
gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca 3120
cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg 3180
accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg 3240
cagatctttt gcccatttta tttttataaa atgggcaggt ggcgtttgtg taaagcaaat 3300
cgacacaatc caaaggggat aaaaggggaa agtgaaactt cccccttttc aagccacatt 3360
gtaatacaag aacgaagtgt tttgtattac aatgtgatag cttgcagtat ttatggtttt 3420
atatggtcta ttttgttgtg aggattgtaa ccgaataggg cgcaatgctt attacaaaat 3480
caatgacaaa gggcgattga ggaatgagcg ctgaggcatt ttatctttga gtaagttatt 3540
gatggatcag aaaaatgtat cacaaattga aacaaagact cactcattta agagaagcta 3600
ctatcatgaa attttgttgt tgtgataagc aacttctaat acacgatttt tagccattac 3660
atcactcgtt tttagagtga tgtgtaagtg cgcattgcac tcttttttta cgaaacaagc 3720
cgaccagcgt ttgaaacttt ttagtttttc atcattctat tttaaaacgt tctaaaactc 3780
gatttaagcg actttaattc gaaactgtct atttgttcaa agggagcatt aagaatgctt 3840
aaacgagctt ttaagggggt ttaaattgat tttgaattga atagcttgtt gtaagttgta 3900
aaaaaaacaa gttaaacaaa gtatcagttt tccatttaag ggttgttagg gcttgccctg 3960
accgtctgta agacgcttga ttgcatgata tgagtattta gctagtcaaa cagttaaaac 4020
agcttatatg agcaattaga gggaatccaa taaattccta aaagcggttt tgatcttttc 4080
ttttagcgag tgaacgctgc aagtaaaatg tgagcgttca ctcgctcact cctttttttg 4140
atgactttga cctttggttt taaatttttg aaaaaaataa aaaataggcg aagcctatta 4200
tatatttatc ttatatattt taatctttta ttcttttgcg tcaaaaaaaa tcaatatttt 4260
caaggcttta tagaattata taccaacaaa aaactgtgta tataccaaca aaaaactgtg 4320
catacaccaa caaaaaactg tgcatatacc aacttctttg tttgtttcgt tggtatataa 4380
tgatataata aaagcatgaa gaatctctct acgaaaagtg tttcttcatg cttatctaaa 4440
ctcactcaca aaggagcagt tttctatgtc tagtatatca aaaaatgaac ctaatcaaaa 4500
gcaggtgcaa accttgaacg aattgtcaaa acgaaaagta gtggaacata attctttaat 4560
taccagtatt gcgaaaatgg ataaaacgcc actgaaaatg tttgaattag ccgtgtcttg 4620
tattaatacc gaagaaccac ccaaagatca tacggtttat ctctcaaaag aagaattgtt 4680
tgcctttttt aaggtatctg ataatgacaa acatagtcgt tttaaacaag cagtagagaa 4740
tatgcaaaaa caagcctttt ttcaaattaa agaagaagta ggtaaaggat ttaaatttag 4800
gagtattgtt cccattccat atgtcgagtg gacagattat catgatgacg taaaaattga 4860
atttcatcgt gaaatcatgc cctacttaat taatctaaaa caaaatttca cgcaacatgc 4920
tttgtctgat attgcagagc tgaatagcaa atactctatt atcttgtacc gttggttatc 4980
catgaattat aaccaatacg agcattatag ttataagggc ggacggagag aagaacaagt 5040
ggaagcctac cgcaatccta ccatttcaat gcgagaatta cgagaaatga cggatacagt 5100
tgatgaatac ccccgctttg atagattaga acatagagtt ttaaaagaac caatagaaga 5160
aattaacgaa aacacctctt ttaacgtgac gtatgacaag ataaaaaaag gacgaagcat 5220
tgattctatt gtctttcata tcacgaaaaa acgtcgagca gatgataaca gctacaagtt 5280
agaagataaa gattatcaat ccgacaaaga ggaaaaatca agaaatgaag ctgacttatt 5340
aaaacaggca atggaaagca agtacacacg attattgatt gaaaactttc tcttatcccc 5400
tcttgaaatg acggacacgg cacttatggc aggtttgcaa aagaacgtct atccgttgta 5460
tgacgagtta aaggaattaa gaggattgaa tggggtcaaa gaccacttgt cttatatatc 5520
tagcaaaaaa gaagcctatt ctaaacgcaa tgtagcgaag tatctgaaaa aagcaatcga 5580
gcaatatcta cctacggtta aaaggcagga cttaaaccat gagtgagaac ttaaaaacga 5640
ttaaagagtt ggctgatgag ttgggcgtat caaaaaagaa aattcattat caagtatcta 5700
aattagatag tgatttgata caaaaattag acggcactat atatctagct gatttcactt 5760
tttgcattct acaaactgca taactcatat 5790
<210> 101
<211> 6350
<212> DNA
<213> Artificial Sequence
<220>
<223> pCIN
<400> 101
gtaaatcgct cctttttagg tggcacaaat gtgaggcatt ttcgctcttt ccggcgaggc 60
tagttaccct taagttattg gtatgactgg ttttaagcgc aaaaaaagtt gctttttcgt 120
acctattaat gtatcgttag aaaaccgact gtaaaaagta cagtcggcat tatctcatat 180
tataaaagcc agtcattagg cctatctgac aattcctgaa tagagttcat aaacaatcct 240
gcatgataac catcacaaac agaatgatgt acctgtaaag atagcggtaa atatattgaa 300
ttacctttat taatgaattt tcctgctgta ataatgggta gaaggtaatt actattatta 360
ttgatattta agttaaaccc agtaaatgaa gtccatggaa taatagaaag agaaaaagca 420
ttttcaggta taggtgtttt gggaaacaat ttccccgaac cattatattt ctctacatca 480
gaaaggtata aatcataaaa ctctttgaag tcattcttta caggagtcca aataccagag 540
aatgttttag atacaccatc aaaaattgta taaagtggct ctaacttatc ccaataacct 600
aactctccgt cgctattgta accagttcta aaagctgtat ttgagtttat cacccttgtc 660
actaagaaaa taaatgcagg gtaaaattta tatccttctt gttttatgtt tcggtataaa 720
acactaatat caatttctgt ggttatacta aaagtcgttt gttggttcaa ataatgatta 780
aatatctctt ttctcttcca attgtctaaa tcaattttat taaagttcat ttgatatgcc 840
tcctaaattt ttatcttgct cttttgtcag agaaatcata actctttttt tcgattctga 900
aatcaccatt taaaaaactc caatcaaata attttataaa attagtgtat cactttgtaa 960
tcataaaaac aacaataaag ctacttaaat atagatttat aaaaaacgtt ggcgaaaacg 1020
ttggcgattc gttggcgatt gaaaaacccc tcaaaccctt gagccagttg ggatagagcg 1080
tttttggcac aaaaattggc actcggcact taatgggggg tcgtagtacg gaagcaaaat 1140
tcgcttcctt tccccccatt tttttccaaa ttccaaattt ttttcaaaaa ttttccagcg 1200
ctaccgctcg gcaaaattgc aagcaatttt taaaatcaaa cccatgaggg aatttcattc 1260
cctcaaactc ccttgagcct cctccaaccg aaatagaagg acgctgcgct tattatttca 1320
ttcagtcatc ggctttcata atctaacaga caacatcttc gctgcaaagc cacgctacgc 1380
tcaagggctt ttacgctacg ataacgcctg ttttaacgat tatgccgata actaaacgaa 1440
ataaacgcta aaacgtctca gaaacgattt tgagacgttt taataaaaaa tcgacttcgt 1500
tcttttttta cctctcggtt atgagttagt tcaaattcgt tctttttagg ttctaaatcg 1560
tgtttttctt ggaattgtgc tgttttatcc tttaccttgt ctacaaaccc cttaaaaacg 1620
tttttaaagg cttttaagcc gtctgtacgt tccttaaggg ctagcataga ttagtttatt 1680
cttgacacta caagctaaat gtggtataat cccatagagt gtggaattgt gagcggataa 1740
caatttcaca caggaaacag accatggaat tcgagctcgg tacccgggga tcctctagag 1800
tcgacctgca ggcatgcaag cttggctgtt ttggcggatg agagaagatt ttcagcctga 1860
tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct ggcggcagta 1920
gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg 1980
gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag 2040
gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg 2100
agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg 2160
cgggcaggac gcccgccata aactgccagg catcaaatta agcagaaggc catcctgacg 2220
gatggccttt ttgcgtttct acaaactctt accggtcctg cagcaatggc aacaacgttg 2280
cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg 2340
atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt 2400
attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg 2460
ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg 2520
gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg 2580
tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa 2640
aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 2700
tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 2760
tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 2820
ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 2880
ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta 2940
gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 3000
aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 3060
ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 3120
agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 3180
aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 3240
aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 3300
ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 3360
cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 3420
tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg 3480
accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc 3540
cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct 3600
gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg 3660
cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat 3720
ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt 3780
catcaccgaa acgcgcgagg cagatctttt gcccatttta tttttataaa atgggcaggt 3840
ggcgtttgtg taaagcaaat cgacacaatc caaaggggat aaaaggggaa agtgaaactt 3900
cccccttttc aagccacatt gtaatacaag aacgaagtgt tttgtattac aatgtgatag 3960
cttgcagtat ttatggtttt atatggtcta ttttgttgtg aggattgtaa ccgaataggg 4020
cgcaatgctt attacaaaat caatgacaaa gggcgattga ggaatgagcg ctgaggcatt 4080
ttatctttga gtaagttatt gatggatcag aaaaatgtat cacaaattga aacaaagact 4140
cactcattta agagaagcta ctatcatgaa attttgttgt tgtgataagc aacttctaat 4200
acacgatttt tagccattac atcactcgtt tttagagtga tgtgtaagtg cgcattgcac 4260
tcttttttta cgaaacaagc cgaccagcgt ttgaaacttt ttagtttttc atcattctat 4320
tttaaaacgt tctaaaactc gatttaagcg actttaattc gaaactgtct atttgttcaa 4380
agggagcatt aagaatgctt aaacgagctt ttaagggggt ttaaattgat tttgaattga 4440
atagcttgtt gtaagttgta aaaaaaacaa gttaaacaaa gtatcagttt tccatttaag 4500
ggttgttagg gcttgccctg accgtctgta agacgcttga ttgcatgata tgagtattta 4560
gctagtcaaa cagttaaaac agcttatatg agcaattaga gggaatccaa taaattccta 4620
aaagcggttt tgatcttttc ttttagcgag tgaacgctgc aagtaaaatg tgagcgttca 4680
ctcgctcact cctttttttg atgactttga cctttggttt taaatttttg aaaaaaataa 4740
aaaataggcg aagcctatta tatatttatc ttatatattt taatctttta ttcttttgcg 4800
tcaaaaaaaa tcaatatttt caaggcttta tagaattata taccaacaaa aaactgtgta 4860
tataccaaca aaaaactgtg catacaccaa caaaaaactg tgcatatacc aacttctttg 4920
tttgtttcgt tggtatataa tgatataata aaagcatgaa gaatctctct acgaaaagtg 4980
tttcttcatg cttatctaaa ctcactcaca aaggagcagt tttctatgtc tagtatatca 5040
aaaaatgaac ctaatcaaaa gcaggtgcaa accttgaacg aattgtcaaa acgaaaagta 5100
gtggaacata attctttaat taccagtatt gcgaaaatgg ataaaacgcc actgaaaatg 5160
tttgaattag ccgtgtcttg tattaatacc gaagaaccac ccaaagatca tacggtttat 5220
ctctcaaaag aagaattgtt tgcctttttt aaggtatctg ataatgacaa acatagtcgt 5280
tttaaacaag cagtagagaa tatgcaaaaa caagcctttt ttcaaattaa agaagaagta 5340
ggtaaaggat ttaaatttag gagtattgtt cccattccat atgtcgagtg gacagattat 5400
catgatgacg taaaaattga atttcatcgt gaaatcatgc cctacttaat taatctaaaa 5460
caaaatttca cgcaacatgc tttgtctgat attgcagagc tgaatagcaa atactctatt 5520
atcttgtacc gttggttatc catgaattat aaccaatacg agcattatag ttataagggc 5580
ggacggagag aagaacaagt ggaagcctac cgcaatccta ccatttcaat gcgagaatta 5640
cgagaaatga cggatacagt tgatgaatac ccccgctttg atagattaga acatagagtt 5700
ttaaaagaac caatagaaga aattaacgaa aacacctctt ttaacgtgac gtatgacaag 5760
ataaaaaaag gacgaagcat tgattctatt gtctttcata tcacgaaaaa acgtcgagca 5820
gatgataaca gctacaagtt agaagataaa gattatcaat ccgacaaaga ggaaaaatca 5880
agaaatgaag ctgacttatt aaaacaggca atggaaagca agtacacacg attattgatt 5940
gaaaactttc tcttatcccc tcttgaaatg acggacacgg cacttatggc aggtttgcaa 6000
aagaacgtct atccgttgta tgacgagtta aaggaattaa gaggattgaa tggggtcaaa 6060
gaccacttgt cttatatatc tagcaaaaaa gaagcctatt ctaaacgcaa tgtagcgaag 6120
tatctgaaaa aagcaatcga gcaatatcta cctacggtta aaaggcagga cttaaaccat 6180
gagtgagaac ttaaaaacga ttaaagagtt ggctgatgag ttgggcgtat caaaaaagaa 6240
aattcattat caagtatcta aattagatag tgatttgata caaaaattag acggcactat 6300
atatctagct gatttcactt tttgcattct acaaactgca taactcatat 6350
<210> 102
<211> 7597
<212> DNA
<213> Artificial Sequence
<220>
<223> pCIN-mvaA
<400> 102
gtaaatcgct cctttttagg tggcacaaat gtgaggcatt ttcgctcttt ccggcgaggc 60
tagttaccct taagttattg gtatgactgg ttttaagcgc aaaaaaagtt gctttttcgt 120
acctattaat gtatcgttag aaaaccgact gtaaaaagta cagtcggcat tatctcatat 180
tataaaagcc agtcattagg cctatctgac aattcctgaa tagagttcat aaacaatcct 240
gcatgataac catcacaaac agaatgatgt acctgtaaag atagcggtaa atatattgaa 300
ttacctttat taatgaattt tcctgctgta ataatgggta gaaggtaatt actattatta 360
ttgatattta agttaaaccc agtaaatgaa gtccatggaa taatagaaag agaaaaagca 420
ttttcaggta taggtgtttt gggaaacaat ttccccgaac cattatattt ctctacatca 480
gaaaggtata aatcataaaa ctctttgaag tcattcttta caggagtcca aataccagag 540
aatgttttag atacaccatc aaaaattgta taaagtggct ctaacttatc ccaataacct 600
aactctccgt cgctattgta accagttcta aaagctgtat ttgagtttat cacccttgtc 660
actaagaaaa taaatgcagg gtaaaattta tatccttctt gttttatgtt tcggtataaa 720
acactaatat caatttctgt ggttatacta aaagtcgttt gttggttcaa ataatgatta 780
aatatctctt ttctcttcca attgtctaaa tcaattttat taaagttcat ttgatatgcc 840
tcctaaattt ttatcttgct cttttgtcag agaaatcata actctttttt tcgattctga 900
aatcaccatt taaaaaactc caatcaaata attttataaa attagtgtat cactttgtaa 960
tcataaaaac aacaataaag ctacttaaat atagatttat aaaaaacgtt ggcgaaaacg 1020
ttggcgattc gttggcgatt gaaaaacccc tcaaaccctt gagccagttg ggatagagcg 1080
tttttggcac aaaaattggc actcggcact taatgggggg tcgtagtacg gaagcaaaat 1140
tcgcttcctt tccccccatt tttttccaaa ttccaaattt ttttcaaaaa ttttccagcg 1200
ctaccgctcg gcaaaattgc aagcaatttt taaaatcaaa cccatgaggg aatttcattc 1260
cctcaaactc ccttgagcct cctccaaccg aaatagaagg acgctgcgct tattatttca 1320
ttcagtcatc ggctttcata atctaacaga caacatcttc gctgcaaagc cacgctacgc 1380
tcaagggctt ttacgctacg ataacgcctg ttttaacgat tatgccgata actaaacgaa 1440
ataaacgcta aaacgtctca gaaacgattt tgagacgttt taataaaaaa tcgacttcgt 1500
tcttttttta cctctcggtt atgagttagt tcaaattcgt tctttttagg ttctaaatcg 1560
tgtttttctt ggaattgtgc tgttttatcc tttaccttgt ctacaaaccc cttaaaaacg 1620
tttttaaagg cttttaagcc gtctgtacgt tccttaaggg ctagcataga ttagtttatt 1680
cttgacacta caagctaaat gtggtataat cccatagagt gtggaattgt gagcggataa 1740
caatttcaca caggaaacag accatggaat tcgagctcgg tacccgggga tccaggaggt 1800
aataaatatg agaaaaaaat tttatcaaat gtcgccacaa gagcgattga attcgttaaa 1860
cttatccgaa aagagtcaag aaattttaag tgagatggcc ctagatacaa ctattttgga 1920
caatctcata gaaaatcaaa tttctgaatt tgaattgcca atgggaattg cccaaaattt 1980
tgttattaat ggacaaagtt ttctaatacc aatggttaca gaggaaccgt cagtaattgc 2040
cgcagcaagt aatggagcaa aaatagctgg aaattttgta gctgaaatta aagaacgttt 2100
aatgcgtggt caaattgtat tttatgatgt taaaaattca gataagattg caaatgaaat 2160
acttgaaaag caagaaaaaa tctttgaaca agccgaactt tcttaccctt caatcgttaa 2220
acgaggtggc ggtttgagag aggtttctag tcggattttc tctagtcaaa agtttttatc 2280
tgttgatgtc aaggttgatg ttaaagacgc catgggtgca aatattatta attcaatctt 2340
agagggaatt gctgaactct ttcggaggtg gtttcctgat gaaaaaattc tgtttagtat 2400
tttatcaaat tatgctactg aatcactagt gaaagtgacc tgtgaaattc cggttgaacg 2460
cctttcaaaa aaagcagatg gatatgaaat tggtcaaaaa attatggctg cttcccaata 2520
ttcaaaaatt gatccttatc gtgctagcac ccataacaaa gggattatga acggtatcaa 2580
tgcggtcatt ttagctacag gaaatgatac tcgggccatc tcagctgcta ttcatgctta 2640
tgcggcaaaa gatggagcct accaagggtt agccaactgg gaacttcaag agaaaatgtt 2700
agttggcgaa ctcgaatttc ctttaccagt ggcaactgtc ggagggggag tcaaggtttt 2760
gccaaaagca caagcggcca tggagattct gggtatcagt gatgctaagg agctcgcaaa 2820
agttattgct gcagtgggac tagctcaaaa cttagccgct ttacgtgccc tagtttcaga 2880
aggaattcaa cagggacaca tgagtctaca agctcgttct ttggctttga gtgtaggagc 2940
acaagcggat gaaatcgcga tattaagtca acaattgcgc aaagaaaaag tgatgaatca 3000
agaagtagcc caaaatttac taaaaaattt gagaaaataa tctagagtcg acctgcaggc 3060
atgcaagctt ggctgttttg gcggatgaga gaagattttc agcctgatac agattaaatc 3120
agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg cggtggtccc 3180
acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggtc 3240
tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcgaaag 3300
actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc 3360
cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc 3420
cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat ggcctttttg 3480
cgtttctaca aactcttacc ggtcctgcag caatggcaac aacgttgcgc aaactattaa 3540
ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata 3600
aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat 3660
ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc 3720
cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata 3780
gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt 3840
actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga 3900
agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 3960
cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 4020
tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 4080
agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 4140
tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 4200
acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 4260
ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 4320
gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 4380
gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 4440
gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 4500
tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 4560
caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 4620
tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc 4680
gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg 4740
agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt 4800
gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt 4860
taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc 4920
gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca 4980
agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg 5040
cgcgaggcag atcttttgcc cattttattt ttataaaatg ggcaggtggc gtttgtgtaa 5100
agcaaatcga cacaatccaa aggggataaa aggggaaagt gaaacttccc ccttttcaag 5160
ccacattgta atacaagaac gaagtgtttt gtattacaat gtgatagctt gcagtattta 5220
tggttttata tggtctattt tgttgtgagg attgtaaccg aatagggcgc aatgcttatt 5280
acaaaatcaa tgacaaaggg cgattgagga atgagcgctg aggcatttta tctttgagta 5340
agttattgat ggatcagaaa aatgtatcac aaattgaaac aaagactcac tcatttaaga 5400
gaagctacta tcatgaaatt ttgttgttgt gataagcaac ttctaataca cgatttttag 5460
ccattacatc actcgttttt agagtgatgt gtaagtgcgc attgcactct ttttttacga 5520
aacaagccga ccagcgtttg aaacttttta gtttttcatc attctatttt aaaacgttct 5580
aaaactcgat ttaagcgact ttaattcgaa actgtctatt tgttcaaagg gagcattaag 5640
aatgcttaaa cgagctttta agggggttta aattgatttt gaattgaata gcttgttgta 5700
agttgtaaaa aaaacaagtt aaacaaagta tcagttttcc atttaagggt tgttagggct 5760
tgccctgacc gtctgtaaga cgcttgattg catgatatga gtatttagct agtcaaacag 5820
ttaaaacagc ttatatgagc aattagaggg aatccaataa attcctaaaa gcggttttga 5880
tcttttcttt tagcgagtga acgctgcaag taaaatgtga gcgttcactc gctcactcct 5940
ttttttgatg actttgacct ttggttttaa atttttgaaa aaaataaaaa ataggcgaag 6000
cctattatat atttatctta tatattttaa tcttttattc ttttgcgtca aaaaaaatca 6060
atattttcaa ggctttatag aattatatac caacaaaaaa ctgtgtatat accaacaaaa 6120
aactgtgcat acaccaacaa aaaactgtgc atataccaac ttctttgttt gtttcgttgg 6180
tatataatga tataataaaa gcatgaagaa tctctctacg aaaagtgttt cttcatgctt 6240
atctaaactc actcacaaag gagcagtttt ctatgtctag tatatcaaaa aatgaaccta 6300
atcaaaagca ggtgcaaacc ttgaacgaat tgtcaaaacg aaaagtagtg gaacataatt 6360
ctttaattac cagtattgcg aaaatggata aaacgccact gaaaatgttt gaattagccg 6420
tgtcttgtat taataccgaa gaaccaccca aagatcatac ggtttatctc tcaaaagaag 6480
aattgtttgc cttttttaag gtatctgata atgacaaaca tagtcgtttt aaacaagcag 6540
tagagaatat gcaaaaacaa gccttttttc aaattaaaga agaagtaggt aaaggattta 6600
aatttaggag tattgttccc attccatatg tcgagtggac agattatcat gatgacgtaa 6660
aaattgaatt tcatcgtgaa atcatgccct acttaattaa tctaaaacaa aatttcacgc 6720
aacatgcttt gtctgatatt gcagagctga atagcaaata ctctattatc ttgtaccgtt 6780
ggttatccat gaattataac caatacgagc attatagtta taagggcgga cggagagaag 6840
aacaagtgga agcctaccgc aatcctacca tttcaatgcg agaattacga gaaatgacgg 6900
atacagttga tgaatacccc cgctttgata gattagaaca tagagtttta aaagaaccaa 6960
tagaagaaat taacgaaaac acctctttta acgtgacgta tgacaagata aaaaaaggac 7020
gaagcattga ttctattgtc tttcatatca cgaaaaaacg tcgagcagat gataacagct 7080
acaagttaga agataaagat tatcaatccg acaaagagga aaaatcaaga aatgaagctg 7140
acttattaaa acaggcaatg gaaagcaagt acacacgatt attgattgaa aactttctct 7200
tatcccctct tgaaatgacg gacacggcac ttatggcagg tttgcaaaag aacgtctatc 7260
cgttgtatga cgagttaaag gaattaagag gattgaatgg ggtcaaagac cacttgtctt 7320
atatatctag caaaaaagaa gcctattcta aacgcaatgt agcgaagtat ctgaaaaaag 7380
caatcgagca atatctacct acggttaaaa ggcaggactt aaaccatgag tgagaactta 7440
aaaacgatta aagagttggc tgatgagttg ggcgtatcaa aaaagaaaat tcattatcaa 7500
gtatctaaat tagatagtga tttgatacaa aaattagacg gcactatata tctagctgat 7560
ttcacttttt gcattctaca aactgcataa ctcatat 7597
<210> 103
<211> 8327
<212> DNA
<213> Artificial Sequence
<220>
<223> pCIN-mvaA-EGFP
<400> 103
gtaaatcgct cctttttagg tggcacaaat gtgaggcatt ttcgctcttt ccggcgaggc 60
tagttaccct taagttattg gtatgactgg ttttaagcgc aaaaaaagtt gctttttcgt 120
acctattaat gtatcgttag aaaaccgact gtaaaaagta cagtcggcat tatctcatat 180
tataaaagcc agtcattagg cctatctgac aattcctgaa tagagttcat aaacaatcct 240
gcatgataac catcacaaac agaatgatgt acctgtaaag atagcggtaa atatattgaa 300
ttacctttat taatgaattt tcctgctgta ataatgggta gaaggtaatt actattatta 360
ttgatattta agttaaaccc agtaaatgaa gtccatggaa taatagaaag agaaaaagca 420
ttttcaggta taggtgtttt gggaaacaat ttccccgaac cattatattt ctctacatca 480
gaaaggtata aatcataaaa ctctttgaag tcattcttta caggagtcca aataccagag 540
aatgttttag atacaccatc aaaaattgta taaagtggct ctaacttatc ccaataacct 600
aactctccgt cgctattgta accagttcta aaagctgtat ttgagtttat cacccttgtc 660
actaagaaaa taaatgcagg gtaaaattta tatccttctt gttttatgtt tcggtataaa 720
acactaatat caatttctgt ggttatacta aaagtcgttt gttggttcaa ataatgatta 780
aatatctctt ttctcttcca attgtctaaa tcaattttat taaagttcat ttgatatgcc 840
tcctaaattt ttatcttgct cttttgtcag agaaatcata actctttttt tcgattctga 900
aatcaccatt taaaaaactc caatcaaata attttataaa attagtgtat cactttgtaa 960
tcataaaaac aacaataaag ctacttaaat atagatttat aaaaaacgtt ggcgaaaacg 1020
ttggcgattc gttggcgatt gaaaaacccc tcaaaccctt gagccagttg ggatagagcg 1080
tttttggcac aaaaattggc actcggcact taatgggggg tcgtagtacg gaagcaaaat 1140
tcgcttcctt tccccccatt tttttccaaa ttccaaattt ttttcaaaaa ttttccagcg 1200
ctaccgctcg gcaaaattgc aagcaatttt taaaatcaaa cccatgaggg aatttcattc 1260
cctcaaactc ccttgagcct cctccaaccg aaatagaagg acgctgcgct tattatttca 1320
ttcagtcatc ggctttcata atctaacaga caacatcttc gctgcaaagc cacgctacgc 1380
tcaagggctt ttacgctacg ataacgcctg ttttaacgat tatgccgata actaaacgaa 1440
ataaacgcta aaacgtctca gaaacgattt tgagacgttt taataaaaaa tcgacttcgt 1500
tcttttttta cctctcggtt atgagttagt tcaaattcgt tctttttagg ttctaaatcg 1560
tgtttttctt ggaattgtgc tgttttatcc tttaccttgt ctacaaaccc cttaaaaacg 1620
tttttaaagg cttttaagcc gtctgtacgt tccttaaggg ctagcataga ttagtttatt 1680
cttgacacta caagctaaat gtggtataat cccatagagt gtggaattgt gagcggataa 1740
caatttcaca caggaaacag accatggaat tcgagctcgg tacccgggga tccaggaggt 1800
aataaatatg agaaaaaaat tttatcaaat gtcgccacaa gagcgattga attcgttaaa 1860
cttatccgaa aagagtcaag aaattttaag tgagatggcc ctagatacaa ctattttgga 1920
caatctcata gaaaatcaaa tttctgaatt tgaattgcca atgggaattg cccaaaattt 1980
tgttattaat ggacaaagtt ttctaatacc aatggttaca gaggaaccgt cagtaattgc 2040
cgcagcaagt aatggagcaa aaatagctgg aaattttgta gctgaaatta aagaacgttt 2100
aatgcgtggt caaattgtat tttatgatgt taaaaattca gataagattg caaatgaaat 2160
acttgaaaag caagaaaaaa tctttgaaca agccgaactt tcttaccctt caatcgttaa 2220
acgaggtggc ggtttgagag aggtttctag tcggattttc tctagtcaaa agtttttatc 2280
tgttgatgtc aaggttgatg ttaaagacgc catgggtgca aatattatta attcaatctt 2340
agagggaatt gctgaactct ttcggaggtg gtttcctgat gaaaaaattc tgtttagtat 2400
tttatcaaat tatgctactg aatcactagt gaaagtgacc tgtgaaattc cggttgaacg 2460
cctttcaaaa aaagcagatg gatatgaaat tggtcaaaaa attatggctg cttcccaata 2520
ttcaaaaatt gatccttatc gtgctagcac ccataacaaa gggattatga acggtatcaa 2580
tgcggtcatt ttagctacag gaaatgatac tcgggccatc tcagctgcta ttcatgctta 2640
tgcggcaaaa gatggagcct accaagggtt agccaactgg gaacttcaag agaaaatgtt 2700
agttggcgaa ctcgaatttc ctttaccagt ggcaactgtc ggagggggag tcaaggtttt 2760
gccaaaagca caagcggcca tggagattct gggtatcagt gatgctaagg agctcgcaaa 2820
agttattgct gcagtgggac tagctcaaaa cttagccgct ttacgtgccc tagtttcaga 2880
aggaattcaa cagggacaca tgagtctaca agctcgttct ttggctttga gtgtaggagc 2940
acaagcggat gaaatcgcga tattaagtca acaattgcgc aaagaaaaag tgatgaatca 3000
agaagtagcc caaaatttac taaaaaattt gagaaaataa tctagagtcg acaggaggta 3060
ataaatatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag 3120
ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc 3180
acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg 3240
cccaccctcg tgaccaccct gacctggggc gtgcagtgct tcagccgcta ccccgaccac 3300
atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc 3360
atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac 3420
accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg 3480
gggcacaagc tggagtacaa ctacatcagc cacaacgtct atatcaccgc cgacaagcag 3540
aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag 3600
ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac 3660
aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac 3720
atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac 3780
aagtaagcat gcaagcttgg ctgttttggc ggatgagaga agattttcgg cctgatacag 3840
attaaatcag aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg 3900
gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt 3960
gtggggtctc ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc 4020
agtcgaaaga ctggggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt 4080
aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg 4140
gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat 4200
ggcctttttg cgtttctaca aactcttacc ggtcctgcag caatggcaac aacgttgcgc 4260
aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg 4320
gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt 4380
gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca 4440
gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat 4500
gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca 4560
gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 4620
atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 4680
ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 4740
ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 4800
ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 4860
ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 4920
ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 4980
tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 5040
tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 5100
tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 5160
tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 5220
gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 5280
tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 5340
ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 5400
gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc 5460
gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt 5520
acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat 5580
gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc 5640
cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg 5700
cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat 5760
caccgaaacg cgcgaggcag atcttttgcc cattttattt ttataaaatg ggcaggtggc 5820
gtttgtgtaa agcaaatcga cacaatccaa aggggataaa aggggaaagt gaaacttccc 5880
ccttttcaag ccacattgta atacaagaac gaagtgtttt gtattacaat gtgatagctt 5940
gcagtattta tggttttata tggtctattt tgttgtgagg attgtaaccg aatagggcgc 6000
aatgcttatt acaaaatcaa tgacaaaggg cgattgagga atgagcgctg aggcatttta 6060
tctttgagta agttattgat ggatcagaaa aatgtatcac aaattgaaac aaagactcac 6120
tcatttaaga gaagctacta tcatgaaatt ttgttgttgt gataagcaac ttctaataca 6180
cgatttttag ccattacatc actcgttttt agagtgatgt gtaagtgcgc attgcactct 6240
ttttttacga aacaagccga ccagcgtttg aaacttttta gtttttcatc attctatttt 6300
aaaacgttct aaaactcgat ttaagcgact ttaattcgaa actgtctatt tgttcaaagg 6360
gagcattaag aatgcttaaa cgagctttta agggggttta aattgatttt gaattgaata 6420
gcttgttgta agttgtaaaa aaaacaagtt aaacaaagta tcagttttcc atttaagggt 6480
tgttagggct tgccctgacc gtctgtaaga cgcttgattg catgatatga gtatttagct 6540
agtcaaacag ttaaaacagc ttatatgagc aattagaggg aatccaataa attcctaaaa 6600
gcggttttga tcttttcttt tagcgagtga acgctgcaag taaaatgtga gcgttcactc 6660
gctcactcct ttttttgatg actttgacct ttggttttaa atttttgaaa aaaataaaaa 6720
ataggcgaag cctattatat atttatctta tatattttaa tcttttattc ttttgcgtca 6780
aaaaaaatca atattttcaa ggctttatag aattatatac caacaaaaaa ctgtgtatat 6840
accaacaaaa aactgtgcat acaccaacaa aaaactgtgc atataccaac ttctttgttt 6900
gtttcgttgg tatataatga tataataaaa gcatgaagaa tctctctacg aaaagtgttt 6960
cttcatgctt atctaaactc actcacaaag gagcagtttt ctatgtctag tatatcaaaa 7020
aatgaaccta atcaaaagca ggtgcaaacc ttgaacgaat tgtcaaaacg aaaagtagtg 7080
gaacataatt ctttaattac cagtattgcg aaaatggata aaacgccact gaaaatgttt 7140
gaattagccg tgtcttgtat taataccgaa gaaccaccca aagatcatac ggtttatctc 7200
tcaaaagaag aattgtttgc cttttttaag gtatctgata atgacaaaca tagtcgtttt 7260
aaacaagcag tagagaatat gcaaaaacaa gccttttttc aaattaaaga agaagtaggt 7320
aaaggattta aatttaggag tattgttccc attccatatg tcgagtggac agattatcat 7380
gatgacgtaa aaattgaatt tcatcgtgaa atcatgccct acttaattaa tctaaaacaa 7440
aatttcacgc aacatgcttt gtctgatatt gcagagctga atagcaaata ctctattatc 7500
ttgtaccgtt ggttatccat gaattataac caatacgagc attatagtta taagggcgga 7560
cggagagaag aacaagtgga agcctaccgc aatcctacca tttcaatgcg agaattacga 7620
gaaatgacgg atacagttga tgaatacccc cgctttgata gattagaaca tagagtttta 7680
aaagaaccaa tagaagaaat taacgaaaac acctctttta acgtgacgta tgacaagata 7740
aaaaaaggac gaagcattga ttctattgtc tttcatatca cgaaaaaacg tcgagcagat 7800
gataacagct acaagttaga agataaagat tatcaatccg acaaagagga aaaatcaaga 7860
aatgaagctg acttattaaa acaggcaatg gaaagcaagt acacacgatt attgattgaa 7920
aactttctct tatcccctct tgaaatgacg gacacggcac ttatggcagg tttgcaaaag 7980
aacgtctatc cgttgtatga cgagttaaag gaattaagag gattgaatgg ggtcaaagac 8040
cacttgtctt atatatctag caaaaaagaa gcctattcta aacgcaatgt agcgaagtat 8100
ctgaaaaaag caatcgagca atatctacct acggttaaaa ggcaggactt aaaccatgag 8160
tgagaactta aaaacgatta aagagttggc tgatgagttg ggcgtatcaa aaaagaaaat 8220
tcattatcaa gtatctaaat tagatagtga tttgatacaa aaattagacg gcactatata 8280
tctagctgat ttcacttttt gcattctaca aactgcataa ctcatat 8327
<210> 104
<211> 2412
<212> DNA
<213> Enterococcus faecalis mvaE
<400> 104
ttgaaaacag tagttattat tgatgcatta cgaacaccaa ttggaaaata taaaggcagc 60
ttaagtcaag taagtgccgt agacttagga acacatgtta caacacaact tttaaaaaga 120
cattccacta tttctgaaga aattgatcaa gtaatctttg gaaatgtttt acaagctgga 180
aatggccaaa atcccgcacg acaaatagca ataaacagcg gtttatctca tgaaattccc 240
gcaatgacag ttaatgaggt ctgcggatca ggaatgaagg ccgttatttt ggcgaaacaa 300
ttgattcaat taggagaagc ggaagtttta attgctggcg ggattgagaa tatgtcccaa 360
gcacctaaat tacaacgatt taattacgaa acagaaagct atgatgcgcc tttttctagt 420
atgatgtacg atgggttaac ggatgccttt agtggtcaag caatgggctt aactgctgaa 480
aatgtggccg aaaagtatca tgtaactaga gaagagcaag atcaattttc tgtacattca 540
caattaaaag cagctcaagc acaagcagaa gggatattcg ctgacgaaat agccccatta 600
gaagtatcag gaacgcttgt ggagaaagat gaagggattc gccctaattc gagcgttgag 660
aagctaggaa cgcttaaaac agtttttaaa gaagacggta ctgtaacagc agggaatgca 720
tcaaccatta atgatggggc ttctgctttg attattgctt cacaagaata tgccgaagca 780
cacggtcttc cttatttagc tattattcga gacagtgtgg aagtcggtat tgatccagcc 840
tatatgggaa tttcgccgat taaagccatt caaaaactgt tagcgcgcaa tcaacttact 900
acggaagaaa ttgatctgta tgaaatcaac gaagcatttg cagcaacttc aatcgtggtc 960
caaagagaac tggctttacc agaggaaaag gtcaacattt atggtggcgg tatttcatta 1020
ggtcatgcga ttggtgccac aggtgctcgt ttattaacga gtttaagtta tcaattaaat 1080
caaaaagaaa agaaatatgg agtggcttct ttatgtatcg gcggtggctt aggactcgct 1140
atgctactag agagacctca gcaaaaaaaa aacagccgat tttatcaaat gagtcctgag 1200
gaacgcctgg cttctcttct taatgaaggc cagatttctg ctgatacaaa aaaagaattt 1260
gaaaatacgg ctttatcttc gcagattgcc aatcatatga ttgaaaatca aatcagtgaa 1320
acagaagtgc cgatgggcgt tggcttacat ttaacagtgg acgaaactga ttatttggta 1380
ccaatggcga cagaagagcc ctcagtgatt gcggctttga gtaatggtgc aaaaatagca 1440
caaggattta aaacagtgaa tcaacaacgt ttaatgcgtg gacaaatcgt tttttacgat 1500
gttgcagacg ccgagtcatt gattgatgaa ctacaagtaa gagaaacgga aatttttcaa 1560
caagcagagt taagttatcc atctatcgtt aaacgcggcg gcggcttaag agatttgcaa 1620
tatcgtgctt ttgatgaatc atttgtatct gtcgactttt tagtagatgt taaggatgca 1680
atgggggcaa atatcgttaa cgctatgttg gaaggtgtgg ccgagttgtt ccgtgaatgg 1740
tttgcggagc aaaagatttt attcagtatt ttaagtaatt atgccacgga gtcggttgtt 1800
acgatgaaaa cggctattcc agtttcacgt ttaagtaagg ggagcaatgg ccgggaaatt 1860
gctgaaaaaa ttgttttagc ttcacgctat gcttcattag atccttatcg ggcagtcacg 1920
cataacaaag ggatcatgaa tggcattgaa gctgtcgttt tagctacagg aaatgataca 1980
cgcgctgtta gcgcttcttg tcatgctttt gcggtgaagg aaggtcgcta ccaaggtttg 2040
actagttgga cgctggatgg cgaacaacta attggtgaaa tttcagttcc gcttgcgtta 2100
gccacggttg gcggtgccac aaaagtctta cctaaatctc aagcagctgc tgatttgtta 2160
gcagtgacgg atgcaaaaga actaagtcga gtagtagcgg ctgttggttt ggcacaaaat 2220
ttagcggcgt tacgggcctt agtctctgaa ggaattcaaa aaggacacat ggctctacaa 2280
gcacgttctt tagcgatgac ggtcggagct actggtaaag aagttgaggc agtcgctcaa 2340
caattaaaac gtcaaaaaac gatgaaccaa gaccgagcct tggctatttt aaatgattta 2400
agaaaacaat aa 2412
<210> 105
<211> 1149
<212> DNA
<213> Enterococcus faecalis mvaS
<400> 105
atgacaattg ggattgataa aattagtttt tttgtgcccc cttattatat tgatatgacg 60
gcactggctg aagccagaaa tgtagaccct ggaaaatttc atattggtat tgggcaagac 120
caaatggcgg tgaacccaat cagccaagat attgtgacat ttgcagccaa tgccgcagaa 180
gcgatcttga ccaaagaaga taaagaggcc attgatatgg tgattgtcgg gactgagtcc 240
agtatcgatg agtcaaaagc ggccgcagtt gtcttacatc gtttaatggg gattcaacct 300
ttcgctcgct ctttcgaaat caaggaagct tgttacggag caacagcagg cttacagtta 360
gctaagaatc acgtagcctt acatccagat aaaaaagtct tggttgtagc agcagatatt 420
gcaaaatatg gattaaattc tggcggtgag cctacacaag gagctggggc ggttgcaatg 480
ttagttgcta gtgaaccgcg catcttggct ttaaaagagg ataatgtgat gctgacgcaa 540
gatatctatg acttttggcg tccaacaggc catccgtatc ctatggtcga tggtcctttg 600
tcaaacgaaa cctacatcca atcttttgcc caagtctggg atgaacataa aaaaagaacc 660
ggtcttgatt ttgcagatta tgatgcttta gcgttccata ttccttacac aaaaatgggc 720
aaaaaagcct tattagcaaa aatctccgac caaactgaag cagaacagga acgaatttta 780
gcccgttatg aagaaagcat catctatagt cgtcgcgtag gaaacttgta tacgggttca 840
ctttatctgg gactcatttc ccttttagaa aatgcaacga ctttaaccgc aggcaatcaa 900
attgggttat tcagttatgg ttctggtgct gtcgctgaat ttttcactgg tgaattagta 960
gctggttatc aaaatcattt acaaaaagaa actcatttag cactgctaga taatcggaca 1020
gaactttcta tcgctgaata tgaagccatg tttgcagaaa ctttagacac agatattgat 1080
caaacgttag aagatgaatt aaaatatagt atttctgcta ttaataatac cgttcgctct 1140
tatcgaaac 1149
<210> 106
<211> 879
<212> DNA
<213> Streptococcus pneumoniae mvaK1
<400> 106
atgacaaaaa aagttggtgt cggtcaggca catagtaaga taattttaat aggggaacat 60
gcggtcgttt acggttatcc tgccatttcc ctgcctcttt tggaggtgga ggtgacctgt 120
aaggtagttc ctgcagagag tccttggcgc ctttatgagg aggatacctt gtccatggcg 180
gtttatgcct cactggagta tttgaatatc acagaagcct gcattcgttg tgagattgac 240
tcggctatcc ctgagaaacg ggggatgggt tcgtcagcgg ctatcagcat agcggccatt 300
cgtgcagtat ttgactacta tcaggctgat ctgcctcatg atgtactaga aatcttggtc 360
aatcgagctg aaatgattgc ccatatgaat cctagtggtt tggatgctaa gacctgtctt 420
agtgaccaac ctattcgctt tatcaagaac gtaggattta cagaacttga gatggattta 480
tccgcctatt tggtgattgc cgatacgggt gtttatggtc atactcgtga agccatccaa 540
gtggttcaaa ataagggcaa ggatgcccta ccgtttttgc atgccttggg agaattaacc 600
cagcaagcag aagttgcgat ttcacaaaaa gatgctgaag gactgggaca aatcctcagt 660
caagcgcatt tacatttaaa agaaattgga gtcagtagcc ctgaggcaga ctttttggtt 720
gaaacgactc ttagccatgg tgctctgggt gccaagatga gcggtggtgg gctaggaggt 780
tgtatcatag ccttggtaac caatttgaca cacgcacaag aactagcaga aagattagaa 840
gagaaaggag ctgttcagac atggatagag agcctgtaa 879
<210> 107
<211> 1011
<212> DNA
<213> Streptococcus pneumoniae mvaK2
<400> 107
atgattgctg ttaaaacttg cggaaaactc tattgggcag gtgaatatgc tattttagag 60
ccagggcagt tagctttgat aaaggatatt cccatctata tgagggctga gattgctttt 120
tctgacagct accgtatcta ttcagatatg tttgatttcg cagtggactt aaggcccaat 180
cctgactaca gcttgattca agaaacgatt gctttgatgg gagacttcct cgctgttcgc 240
ggtcagaatt taagaccttt ttccctaaaa atctgtggca aaatggaacg agaagggaaa 300
aagtttggtc taggttctag tggcagcgtc gttgtcttgg ttgtcaaggc tttactggct 360
ctctataatc tttcggttga tcagaatctc ttgttcaagc tgactagcgc tgtcttgctc 420
aagcgaggag acaatggttc catgggcgac cttgcctgta ttgtggcaga ggatttggtt 480
ctttaccagt catttgatcg ccagaaggcg gctgcttggt tagaagaaga aaacttggcg 540
acagttctgg agcgtgattg gggatttttt atctcacaag tgaaaccaac tttagaatgt 600
gatttcttag tgggatggac caaggaagtg gctgtatcga gtcacatggt ccagcaaatc 660
aagcaaaata tcaatcaaaa ttttttaagt tcctcaaaag aaacggtggt ttctttggtc 720
gaagccttgg agcaggggaa agccgaaaaa gttatcgagc aagtagaagt agccagcaag 780
cttttagaag gcttgagtac agatatttac acgcctttgc ttagacagtt gaaagaagcc 840
agtcaagatt tgcaggccgt tgccaagagt agtggtgctg gtggtggtga ctgtggcatc 900
gccctgagtt ttgatgcgca atcttctcga aacactttaa aaaatcgttg ggccgatctg 960
gggattgagc tcttatatca agaaaggata ggacatgacg acaaatcgta a 1011
<210> 108
<211> 954
<212> DNA
<213> Streptococcus pneumoniae mvaD
<400> 108
atggatagag agcctgtaac agtacgttcc tacgcaaata ttgctattat caaatattgg 60
ggaaagaaaa aagaaaaaga gatggtgcct gctactagca gtatttctct aactttggaa 120
aatatgtata cagagacgac cttgtcgcct ttaccagcca atgtaacagc tgacgaattt 180
tacatcaatg gtcagctaca aaatgaggtc gagcatgcca agatgagtaa gattattgac 240
cgttatcgtc cagctggtga gggctttgtc cgtatcgata ctcaaaacaa tatgcctacg 300
gcagcgggcc tgtcctcaag ttctagtggt ttgtccgccc tggtcaaggc ttgtaatgct 360
tatttcaagc ttggattgga tagaagtcag ttggcacagg aagccaaatt tgcctcaggc 420
tcttcttctc ggagttttta tggaccacta ggagcctggg ataaggatag tggagaaatt 480
taccctgtag agacagactt gaaactagct atgattatgt tggtgctaga ggacaagaaa 540
aaaccaatct ctagccgtga cgggatgaaa ctttgtgtgg aaacctcgac gacttttgac 600
gactgggttc gtcagtctga gaaggactat caggatatgc tgatttatct caaggaaaat 660
gattttgcca agattggaga attaacggag aaaaatgctc tggctatgca tgctacgaca 720
aagactgcta gtccagcctt ttcttatctg acggatgcct cttatgaggc tatggccttt 780
gttcgccagc ttcgtgagaa aggagaggcc tgctacttta ccatggatgc tggtcccaat 840
gttaaggtct tctgtcagga gaaagacttg gagcatttgt cagaaatttt cggtcagcgt 900
tatcgcttga ttgtgtcaaa aacaaaggat ttgagtcaag atgattgctg ttaa 954
<210> 109
<211> 549
<212> DNA
<213> Escherichia coli idi
<400> 109
atgcaaacgg aacacgtcat tttattgaat gcacagggag ttcccacggg tacgctggaa 60
aagtatgccg cacacacggc agacacccgc ttacatctcg cgttctccag ttggctgttt 120
aatgccaaag gacaattatt agttacccgc cgcgcactga gcaaaaaagc atggcctggc 180
gtgtggacta actcggtttg tgggcaccca caactgggag aaagcaacga agacgcagtg 240
atccgccgtt gccgttatga gcttggcgtg gaaattacgc ctcctgaatc tatctatcct 300
gactttcgct accgcgccac cgatccgagt ggcattgtgg aaaatgaagt gtgtccggta 360
tttgccgcac gcaccactag tgcgttacag atcaatgatg atgaagtgat ggattatcaa 420
tggtgtgatt tagcagatgt attacacggt attgatgcca cgccgtgggc gttcagtccg 480
tggatggtga tgcaggcgac aaatcgcgaa gccagaaaac gattatctgc atttacccag 540
cttaaataa 549
<210> 110
<211> 915
<212> DNA
<213> Haematococcus pluvialis ipiHp1
<400> 110
atgcttcgtt cgttgctcag aggcctcacg catatccccc gcgtgaactc cgcccagcag 60
cccagctgtg cacacgcgcg actccagttt aagctcagga gcatgcagat gacgctcatg 120
cagcccagca tctcagccaa tctgtcgcgc gccgaggacc gcacagacca catgaggggt 180
gcaagcacct gggcaggcgg gcagtcgcag gatgagctga tgctgaagga cgagtgcatc 240
ttggtggatg ttgaggacaa catcacaggc catgccagca agctggagtg tcacaagttc 300
ctaccacatc agcctgcagg cctgctgcac cgggccttct ctgtgttcct gtttgacgat 360
caggggcgac tgctgctgca acagcgtgca cgctcaaaaa tcaccttccc aagtgtgtgg 420
acgaacacct gctgcagcca ccctttacat gggcagaccc cagatgaggt ggaccaacta 480
agccaggtgg ccgacggaac agtacctggc gcaaaggctg ctgccatccg caagttggag 540
cacgagctgg ggataccagc gcaccagctg ccggcaagcg cgtttcgctt cctcacgcgt 600
ttgcactact gtgccgcgga cgtgcagcca gctgcgacac aatcagcgct ctggggcgag 660
cacgaaatgg actacatctt gttcatccgg gccaacgtca ccttggcgcc caaccctgac 720
gaggtggacg aagtcaggta cgtgacgcaa gaggagctgc ggcagatgat gcagccggac 780
aacgggctgc aatggtcgcc gtggtttcgc atcatcgccg cgcgcttcct tgagcgttgg 840
tgggctgacc tggacgcggc cctaaacact gacaaacacg aggattgggg aacggtgcat 900
cacatcaacg aagcg 915
<210> 111
<211> 924
<212> DNA
<213> pantoea agglomerans crtE
<400> 111
atggtgagtg gcagtaaagc gggcgtttcg cctcatcgcg aaatagaagt aatgagacaa 60
tccattgacg atcacctggc tggcctgtta cctgaaaccg acagccagga tatcgtcagc 120
cttgcgatgc gtgaaggcgt catggcaccc ggtaaacgga tccgtccgct gctgatgctg 180
ctggccgccc gcgacctccg ctaccagggc agtatgccta cgctgctcga tctcgcctgc 240
gccgttgaac tgacccatac cgcgtcgctg atgctcgacg acatgccctg catggacaac 300
gccgagctgc gccgcggtca gcccactacc cacaaaaaat ttggtgagag cgtggcgatc 360
cttgcctccg ttgggctgct ctctaaagcc tttggtctga tcgccgccac cggcgatctg 420
ccgggggaga ggcgtgccca ggcggtcaac gagctctcta ccgccgtggg cgtgcagggc 480
ctggtactgg ggcagtttcg cgatcttaac gatgccgccc tcgaccgtac ccctgacgct 540
atcctcagca ccaaccacct caagaccggc attctgttca gcgcgatgct gcagatcgtc 600
gccattgctt ccgcctcgtc gccgagcacg cgagagacgc tgcacgcctt cgccctcgac 660
ttcggccagg cgtttcaact gctggacgat ctgcgtgacg atcacccgga aaccggtaaa 720
gatcgcaata aggacgcggg aaaatcgacg ctggtcaacc ggctgggcgc agacgcggcc 780
cggcaaaagc tgcgcgagca tattgattcc gccgacaaac acctcacttt tgcctgtccg 840
cagggcggcg ccatccgaca gtttatgcat ctgtggtttg gccatcacct tgccgactgg 900
tcaccggtca tgaaaatcgc ctga 924
<210> 112
<211> 930
<212> DNA
<213> pantoea agglomerans crtB
<400> 112
atgagccaac cgccgctgct tgaccacgcc acgcagacca tggccaacgg ctcgaaaagt 60
tttgccaccg ctgcgaagct gttcgacccg gccacccgcc gtagcgtgct gatgctctac 120
acctggtgcc gccactgcga tgacgtcatt gacgaccaga cccacggctt cgccagcgag 180
gccgcggcgg aggaggaggc cacccagcgc ctggcccggc tgcgcacgct gaccctggcg 240
gcgtttgaag gggccgagat gcaggatccg gccttcgctg cctttcagga ggtggcgctg 300
acccacggta ttacgccccg catggcgctc gatcacctcg acggctttgc gatggacgtg 360
gctcagaccc gctatgtcac ctttgaggat acgctgcgct actgctatca cgtggcgggc 420
gtggtgggtc tgatgatggc cagggtgatg ggcgtgcggg atgagcgggt gctggatcgc 480
gcctgcgatc tggggctggc cttccagctg acgaatatcg cccgggatat tattgacgat 540
gcggctattg accgctgcta tctgcccgcc gagtggctgc aggatgccgg gctgaccccg 600
gagaactatg ccgcgcggga gaatcgggcc gcgctggcgc gggtggcgga gcggcttatt 660
gatgccgcag agccgtacta catctcctcc caggccgggc tacacgatct gccgccgcgc 720
tgcgcctggg cgatcgccac cgcccgcagc gtctaccggg agatcggtat taaggtaaaa 780
gcggcgggag gcagcgcctg ggatcgccgc cagcacacca gcaaaggtga aaaaattgcc 840
atgctgatgg cggcaccggg gcaggttatt cgggcgaaga cgacgagggt gacgccgcgt 900
ccggccggtc tttggcagcg tcccgtttag 930
<210> 113
<211> 1479
<212> DNA
<213> pantoea agglomerans crtI
<400> 113
atgaaaaaaa ccgttgtgat tggcgcaggc tttggtggcc tggcgctggc gattcgcctg 60
caggcggcag ggatcccaac cgtactgctg gagcagcggg acaagcccgg cggtcgggcc 120
tacgtctggc atgaccaggg ctttaccttt gacgccgggc cgacggtgat caccgatcct 180
accgcgcttg aggcgctgtt caccctggcc ggcaggcgca tggaggatta cgtcaggctg 240
ctgccggtaa aacccttcta ccgactctgc tgggagtccg ggaagaccct cgactatgct 300
aacgacagcg ccgagcttga ggcgcagatt acccagttca acccccgcga cgtcgagggc 360
taccggcgct ttctggctta ctcccaggcg gtattccagg agggatattt gcgcctcggc 420
agcgtgccgt tcctctcttt tcgcgacatg ctgcgcgccg ggccgcagct gcttaagctc 480
caggcgtggc agagcgtcta ccagtcggtt tcgcgcttta ttgaggatga gcatctgcgg 540
caggccttct cgttccactc cctgctggta ggcggcaacc ccttcaccac ctcgtccatc 600
tacaccctga tccacgccct tgagcgggag tggggggtct ggttccctga gggcggcacc 660
ggggcgctgg tgaacggcat ggtgaagctg tttaccgatc tgggcgggga gatcgaactc 720
aacgcccggg tcgaagagct ggtggtggcc gataaccgcg taagccaggt ccggctggcg 780
gatggtcgga tctttgacac cgacgccgta gcctcgaacg ctgacgtggt gaacacctat 840
aaaaagctgc tcggccacca tccggtgggg cagaagcggg cggcagcgct ggagcgcaag 900
agcatgagca actcgctgtt tgtgctctac ttcggcctga accagcctca ttcccagctg 960
gcgcaccata ccatctgttt tggtccccgc taccgggagc tgatcgacga gatctttacc 1020
ggcagcgcgc tggcggatga cttctcgctc tacctgcact cgccctgcgt gaccgatccc 1080
tcgctcgcgc ctcccggctg cgccagcttc tacgtgctgg ccccggtgcc gcatcttggc 1140
aacgcgccgc tggactgggc gcaggagggg ccgaagctgc gcgaccgcat ctttgactac 1200
cttgaagagc gctatatgcc cggcctgcgt agccagctgg tgacccagcg gatctttacc 1260
ccggcagact tccacgacac gctggatgcg catctgggat cggccttctc catcgagccg 1320
ctgctgaccc aaagcgcctg gttccgcccg cacaaccgcg acagcgacat tgccaacctc 1380
tacctggtgg gcgcaggtac tcaccctggg gcgggcattc ctggcgtagt ggcctcggcg 1440
aaagccaccg ccagcctgat gattgaggat ctgcaatga 1479
<210> 114
<211> 1146
<212> DNA
<213> pantoea ananatis crtY
<400> 114
atgcaaccgc attatgatct gattctcgtg ggggctggac tcgcgaatgg ccttatcgcc 60
ctgcgtcttc agcagcagca acctgatatg cgtattttgc ttatcgacgc cgcaccccag 120
gcgggcggga atcatacgtg gtcatttcac cacgatgatt tgactgagag ccaacatcgt 180
tggatagctc cgctggtggt tcatcactgg cccgactatc aggtacgctt tcccacacgc 240
cgtcgtaagc tgaacagcgg ctacttttgt attacttctc agcgtttcgc tgaggtttta 300
cagcgacagt ttggcccgca cttgtggatg gataccgcgg tcgcagaggt taatgcggaa 360
tctgttcggt tgaaaaaggg tcaggttatc ggtgcccgcg cggtgattga cgggcggggt 420
tatgcggcaa attcagcact gagcgtgggc ttccaggcgt ttattggcca ggaatggcga 480
ttgagccacc cgcatggttt atcgtctccc attatcatgg atgccacggt cgatcagcaa 540
aatggttatc gcttcgtgta cagcctgccg ctctcgccga ccagattgtt aattgaagac 600
acgcactata ttgataatgc gacattagat cctgaatgcg cgcggcaaaa tatttgcgac 660
tatgccgcgc aacagggttg gcagcttcag acactgctgc gagaagaaca gggcgcctta 720
cccattactc tgtcgggcaa tgccgacgca ttctggcagc agcgccccct ggcctgtagt 780
ggattacgtg ccggtctgtt ccatcctacc accggctatt cactgccgct ggcggttgcc 840
gtggccgacc gcctgagtgc acttgatgtc tttacgtcgg cctcaattca ccatgccatt 900
acgcattttg cccgcgagcg ctggcagcag cagggctttt tccgcatgct gaatcgcatg 960
ctgtttttag ccggacccgc cgattcacgc tggcgggtta tgcagcgttt ttatggttta 1020
cctgaagatt taattgcccg tttttatgcg ggaaaactca cgctgaccga tcggctacgt 1080
attctgagcg gcaagccgcc tgttccggta ttagcagcat tgcaagccat tatgacgact 1140
catcgt 1146
<210> 115
<211> 828
<212> DNA
<213> uncultured marine bacterium 66A03 SR
<400> 115
atgggtctga tgctgattga ttggtgtgca ctggctctgg ttgttttcat tggcctgccg 60
cacggcgcgc tggatgctgc catttctttt tctatgatct cttctgcaaa acgcattgct 120
cgtctggctg gtattctgct gatctatctg ctgctggcga ccgcgttctt cctgatctgg 180
tatcagctgc cagcgtttag cctgctgatc ttcctgctga tctccattat ccactttggt 240
atggcagact tcaacgcgtc cccaagcaaa ctgaaatggc cgcatatcat cgcccacggc 300
ggtgttgtta ctgtttggct gccgctgatc cagaaaaacg aagtaactaa actgtttagc 360
atcctgacta acggtccgac tccgatcctg tgggacatcc tgctgatttt cttcctgtgt 420
tggtctattg gcgtgtgtct gcacacgtac gaaaccctgc gctctaaaca ttacaacatc 480
gcctttgaac tgatcggtct gattttcctg gcgtggtatg cgccgcctct ggttacgttt 540
gccacttact tctgcttcat tcattcccgt cgccacttct cctttgtgtg gaagcagctg 600
caacacatgt cttccaaaaa gatgatgatt ggcagcgcga ttatcctgtc ctgtacctct 660
tggctgatcg gcggtggtat ctatttcttc ctgaactcca aaatgatcgc ctctgaggct 720
gcgctgcaga ctgtgttcat cggtctggcg gcactgaccg tgccgcacat gattctgatc 780
gacttcatct tccgtccgca ctcttcccgt atcaaaatca aaaactaa 828
<210> 116
<211> 810
<212> DNA
<213> Escherichia coli MG1655 YbbO
<400> 116
atgactcata aagcaacgga gatcctgaca ggtaaagtta tgcaaaaatc ggtcttaatt 60
accggatgtt ccagtggaat tggcctggaa agcgcgctcg aattaaaacg ccagggtttt 120
catgtgctgg caggttgccg gaaaccggat gatgttgagc gcatgaacag catgggattt 180
accggcgtgt tgatcgatct ggattcacca gaaagtgttg atcgcgcagc cgacgaggtg 240
atcgccctga ccgataattg tctgtatggg atctttaaca atgccggatt cggcatgtat 300
ggcccccttt ccaccatcag ccgtgcgcag atggaacagc agttttccgc caactttttc 360
ggcgcacacc agctcaccat gcgcctgtta cccgcgatgt taccgcacgg tgaagggcgt 420
attgtgatga catcatcggt gatgggatta atctccacgc cgggtcgtgg cgcttacgcg 480
gccagtaaat atgcgctgga ggcgtggtca gatgcactgc gcatggagct gcgccacagc 540
ggaattaaag tcagcctgat cgaacccggt cccattcgta ctcgcttcac cgacaacgtc 600
aaccagacgc aaagtgataa accagtcgaa aatcccggca tcgccgcccg ctttacgttg 660
ggaccggaag cggtggtgga caaagtacgc catgctttta ttagcgagaa gccgaagatg 720
cgctatccgg tgacgctggt gacctgggcg gtaatggtgc ttaagcgcct gctgccgggg 780
cgcgtgatgg acaaaatatt gcaggggtaa 810
<210> 117
<211> 1863
<212> DNA
<213> Escherichia coli dxs
<400> 117
atgagttttg atattgccaa atacccgacc ctggcactgg tcgactccac ccaggagtta 60
cgactgttgc cgaaagagag tttaccgaaa ctctgcgacg aactgcgccg ctatttactc 120
gacagcgtga gccgttccag cgggcacttc gcctccgggc tgggcacggt cgaactgacc 180
gtggcgctgc actatgtcta caacaccccg tttgaccaat tgatttggga tgtggggcat 240
caggcttatc cgcataaaat tttgaccgga cgccgcgaca aaatcggcac catccgtcag 300
aaaggcggtc tgcacccgtt cccgtggcgc ggcgaaagcg aatatgacgt attaagcgtc 360
gggcattcat caacctccat cagtgccgga attggtattg cggttgctgc cgaaaaagaa 420
ggcaaaaatc gccgcaccgt ctgtgtcatt ggcgatggcg cgattaccgc aggcatggcg 480
tttgaagcga tgaatcacgc gggcgatatc cgtcctgata tgctggtgat tctcaacgac 540
aatgaaatgt cgatttccga aaatgtcggc gcgctcaaca accatctggc acagctgctt 600
tccggtaagc tttactcttc actgcgcgaa ggcgggaaaa aagttttctc tggcgtgccg 660
ccaattaaag agctgctcaa acgcaccgaa gaacatatta aaggcatggt agtgcctggc 720
acgttgtttg aagagctggg ctttaactac atcggcccgg tggacggtca cgatgtgctg 780
gggcttatca ccacgctaaa gaacatgcgc gacctgaaag gcccgcagtt cctgcatatc 840
atgaccaaaa aaggtcgtgg ttatgaaccg gcagaaaaag acccgatcac tttccacgcc 900
gtgcctaaat ttgatccctc cagcggttgt ttgccgaaaa gtagcggcgg tttgccgagc 960
tattcaaaaa tctttggcga ctggttgtgc gaaacggcag cgaaagacaa caagctgatg 1020
gcgattactc cggcgatgcg tgaaggttcc ggcatggtcg agttttcacg taaattcccg 1080
gatcgctact tcgacgtggc aattgccgag caacacgcgg tgacctttgc tgcgggtctg 1140
gcgattggtg ggtacaaacc cattgtcgcg atttactcca ctttcctgca acgcgcctat 1200
gatcaggtgc tgcatgacgt ggcgattcaa aagcttccgg tcctgttcgc catcgaccgc 1260
gcgggcattg ttggtgctga cggtcaaacc catcagggtg cttttgatct ctcttacctg 1320
cgctgcatac cggaaatggt cattatgacc ccgagcgatg aaaacgaatg tcgccagatg 1380
ctctataccg gctatcacta taacgatggc ccgtcagcgg tgcgctaccc gcgtggcaac 1440
gcggtcggcg tggaactgac gccgctggaa aaactaccaa ttggcaaagg cattgtgaag 1500
cgtcgtggcg agaaactggc gatccttaac tttggtacgc tgatgccaga agcggcgaaa 1560
gtcgccgaat cgctgaacgc cacgctggtc gatatgcgtt ttgtgaaacc gcttgatgaa 1620
gcgttaattc tggaaatggc cgccagccat gaagcgctgg tcaccgtaga agaaaacgcc 1680
attatgggcg gcgcaggcag cggcgtgaac gaagtgctga tggcccatcg taaaccagta 1740
cccgtgctga acattggcct gccggacttc tttattccgc aaggaactca ggaagaaatg 1800
cgcgccgaac tcggcctcga tgccgctggt atggaagcca aaatcaaggc ctggctggca 1860
taa 1863
<210> 118
<211> 1197
<212> DNA
<213> Escherichia coli dxr
<400> 118
atgaagcaac tcaccattct gggctcgacc ggctcgattg gttgcagcac gctggacgtg 60
gtgcgccata atcccgaaca cttccgcgta gttgcgctgg tggcaggcaa aaatgtcact 120
cgcatggtag aacagtgcct ggaattctct ccccgctatg ccgtaatgga cgatgaagcg 180
agtgcgaaac ttcttaaaac gatgctacag caacagggta gccgcaccga agtcttaagt 240
gggcaacaag ccgcttgcga tatggcagcg cttgaggatg ttgatcaggt gatggcagcc 300
attgttggcg ctgctgggct gttacctacg cttgctgcga tccgcgcggg taaaaccatt 360
ttgctggcca ataaagaatc actggttacc tgcggacgtc tgtttatgga cgccgtaaag 420
cagagcaaag cgcaattgtt accggtcgat agcgaacata acgccatttt tcagagttta 480
ccgcaaccta tccagcataa tctgggatac gctgaccttg agcaaaatgg cgtggtgtcc 540
attttactta ccgggtctgg tggccctttc cgtgagacgc cattgcgcga tttggcaaca 600
atgacgccgg atcaagcctg ccgtcatccg aactggtcga tggggcgtaa aatttctgtc 660
gattcggcta ccatgatgaa caaaggtctg gaatacattg aagcgcgttg gctgtttaac 720
gccagcgcca gccagatgga agtgctgatt cacccgcagt cagtgattca ctcaatggtg 780
cgctatcagg acggcagtgt tctggcgcag ctgggggaac cggatatgcg tacgccaatt 840
gcccacacca tggcatggcc gaatcgcgtg aactctggcg tgaagccgct cgatttttgc 900
aaactaagtg cgttgacatt tgccgcaccg gattatgatc gttatccatg cctgaaactg 960
gcgatggagg cgttcgaaca aggccaggca gcgacgacag cattgaatgc cgcaaacgaa 1020
atcaccgttg ctgcttttct tgcgcaacaa atccgcttta cggatatcgc tgcgttgaat 1080
ttatccgtac tggaaaaaat ggatatgcgc gaaccacaat gtgtggacga tgtgttatct 1140
gttgatgcga acgcgcgtga agtcgccaga aaagaggtga tgcgtctcgc aagctga 1197
<210> 119
<211> 900
<212> DNA
<213> Escherichia coli ispA
<400> 119
atggactttc cgcagcaact cgaagcctgc gttaagcagg ccaaccaggc gctgagccgt 60
tttatcgccc cactgccctt tcagaacact cccgtggtcg aaaccatgca gtatggcgca 120
ttattaggtg gtaagcgcct gcgacctttc ctggtttatg ccaccggtca tatgttcggc 180
gttagcacaa acacgctgga cgcacccgct gccgccgttg agtgtatcca cgcttactca 240
ttaattcatg atgatttacc ggcaatggat gatgacgatc tgcgtcgcgg tttgccaacc 300
tgccatgtga agtttggcga agcaaacgcg attctcgctg gcgacgcttt acaaacgctg 360
gcgttctcga ttttaagcga tgccgatatg ccggaagtgt cggaccgcga cagaatttcg 420
atgatttctg aactggcgag cgccagtggt attgccggaa tgtgcggtgg tcaggcatta 480
gatttagacg cggaaggcaa acacgtacct ctggacgcgc ttgagcgtat tcatcgtcat 540
aaaaccggcg cattgattcg cgccgccgtt cgccttggtg cattaagcgc cggagataaa 600
ggacgtcgtg ctctgccggt actcgacaag tatgcagaga gcatcggcct tgccttccag 660
gttcaggatg acatcctgga tgtggtggga gatactgcaa cgttgggaaa acgccagggt 720
gccgaccagc aacttggtaa aagtacctac cctgcacttc tgggtcttga gcaagcccgg 780
aagaaagccc gggatctgat cgacgatgcc cgtcagtcgc tgaaacaact ggctgaacag 840
tcactcgata cctcggcact ggaagcgcta gcggactaca tcatccagcg taataaataa 900
900
<210> 120
<211> 1656
<212> DNA
<213> Clausena lansium STS
<400> 120
atgtcaactc aacaagtttc atcagagaac attgttcgta acgctgcgaa tttccatcct 60
aatatatggg gaaaccattt cctcacatgt ccttctcaga cgattgatag ttggactcaa 120
cagcaccaca aagaactgaa agaagaggtg aggaaaatga tggtgtctga tgcaaataaa 180
cctgcccaga gattgcgctt gattgatact gtccaaaggc taggtgtggc ttaccacttt 240
gaaaaggaga ttgatgatgc attggagaaa ataggtcatg acccttttga tgataaagat 300
gatctctaca ttgtctctct ttgttttcga ttgctgaggc agcatggaat taagatatca 360
tgtgatgtgt ttgagaagtt taaagatgac gatggaaaat tcaaggcatc attgatgaat 420
gatgttcaag gcatgctaag tttatatgag gcagcacacc tagccattca cggagaagat 480
attttagatg aagcaattgt tttcacgacc actcacctta agtcaacggt atctaattct 540
cctgtaaact ctacttttgc tgaacaaata cgtcattctc tcagagttcc tctccgtaaa 600
gctgtaccta ggttagagtc gaggtatttc ttggatatct attcaagaga tgatttgcac 660
gataaaactt tgctcaattt cgcaaagtta gactttaata tactacaagc aatgcaccag 720
aaggaagcaa gtgagatgac caggtggtgg agagattttg acttccttaa aaagctgcct 780
tatataagag acagagtcgt ggagctatat ttttggattc tggtgggagt gtcttatcag 840
cccaaattca gcactggtag aatttttttg tccaaaataa tatgccttga gaccctcgta 900
gatgatacat ttgacgccta cggtactttt gacgagctcg caatctttac tgaagcagtt 960
acaagatggg accttggcca cagagatgca ctaccagaat acatgaaatt cattttcaag 1020
acactcattg atgtctacag tgaagctgag caagaactgg caaaggaagg gagatcatac 1080
agcatacact atgcaatacg atcgttccaa gaactagtta tgaagtactt ctgcgaagcc 1140
aagtggttaa ataaaggtta tgttccgagc ctggacgatt ataaatcagt ttcattaaga 1200
agtatcggtt ttttaccgat agcggtagct tccttcgttt tcatgggtga tattgcaact 1260
aaggaggtct ttgaatggga aatgaataac cctaagatca taatagccgc agaaacgatt 1320
ttcagattcc tggatgacat agcaggccat aggtttgagc aaaagagaga acatagtcca 1380
tcagctattg aatgctacaa gaatcaacat ggagtgtctg aggaagaggc agttaaagcg 1440
ttgtcgttag aagttgctaa tagttggaaa gatataaatg aggagctgct tctcaaccca 1500
atggctattc ctttacctct gcttcaggtg attcttgatc tctcacgttc ggccgatttt 1560
atgtacggta atgctcaaga tcgcttcacg cattcaacga tgatgaaaga ccaagttgat 1620
ttggtgctga aggaccccgt taagcttgac gattaa 1656
<210> 121
<211> 1719
<212> DNA
<213> Artificial Sequence
<220>
<223> Matricaria recutita MrBBS
<400> 121
atgtcaaccc tgtcagtctc cacgccgtcc ttctcatcgt cgccgctgtc ctcagtgaac 60
aaaaatagca cgaaacaaca cgttacgcgc aatagtgtga tctttcatga ttccatttgg 120
ggcgaccagt tcctggaata caaagaaaaa ttcaacgttg caaccgaaaa acaactgatt 180
gaagaactga aagaagaagt ccgcaatgaa ctgatgatcc gtgcgtgcaa cgaagccagt 240
cgctatatta aactgatcca gctgattgat gtggttgaac gtctgggcct ggcctaccac 300
tttgaaaaag aaatcgaaga atccctgcaa catatttatg ttacctacgg tcacaaatgg 360
acgaactaca acaacatcga aagcctgtct ctgtggtttc gcctgctgcg tcagaacggt 420
tttaatgtca gctctgatat cttcgaaaac catattgacg aaaaaggcaa tttccaagaa 480
agtctgtgca acgatccgca aggcatgctg gcactgtatg aagcggccta catgcgcgtg 540
gaaggcgaaa ttatcctgga caaagctctg gaatttacca aactgcacct gggtattatc 600
agcaacgatc cgtcttgtga cagttccctg cgtacggaaa ttaaacaggc gctgaaacag 660
ccgctgcgtc gccgtctgcc gcgtctggaa gcagttcgtt atattgctat ctaccagcaa 720
aaagcgagtc attccgaagt cctgctgaaa ctggccaaac tggatttcaa tgtgctgcag 780
gaaatgcaca aagacgaact gtcacaaatt tgtaaatggt ggaaagacct ggacatccgt 840
aacaaactgc cgtatgttcg cgatcgtctg attgaaggct atttttggat tctgggtatt 900
tacttcgaac cgcagcattc gcgcacccgt atgtttctga tgaaaacgtg catgtggctg 960
atcgtcctgg atgacacctt tgataattat ggcacgtacg aagaactgga aattttcacc 1020
caggcggtgg aacgctggag catcacgtgt ctggatgaac tgccggaata catgaaactg 1080
atctaccatg aacagttccg tgtgcaccaa gaaatggaag aatctctgga aaaagaaggt 1140
aaagcatacc agatccatta catcaaagaa atggctaaag aaggcacccg ctctctgctg 1200
ctggaagcga aatggctgaa agaaggttat atgccgacgc tggatgaata cctgtcaaac 1260
tcgctggtta cctgcggcta tgcactgatg acggctcgct catacgttgc ccgtgatgac 1320
ggtattgtca ccgaagatgc gtttaaatgg gtggccacgc acccgccgat cgttaaagca 1380
gcttgtaaaa ttctgcgtct gatggatgac atcgcgaccc ataaagaaga acaggaacgc 1440
ggccacatcg cctcatcgat tgaatgctat cgtaaagaaa ccggtgcatc tgaagaagaa 1500
gcgtgtatgg attttctgaa acaggtcgaa gacggctgga aagtgatcaa tcaagaatca 1560
ctgatgccga ccgatgtgcc gttcccgctg ctgatcccgg cgattaacct ggcccgcgtt 1620
tcggacacgc tgtataaaga taacgacggt tacaatcatg ctgataaaga agtgattggt 1680
tacattaaaa gtctgtttgt ccacccgatg attgtgtga 1719
<210> 122
<211> 717
<212> DNA
<213> Artificial Sequence
<220>
<223> EGFP
<400> 122
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 60
ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac 120
ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc 180
ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag 240
cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 300
ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 360
gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 420
aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac 480
ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc 540
gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac 600
tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc 660
ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaag 717
<210> 123
<211> 1233
<212> DNA
<213> Artificial Sequence
<220>
<223> Lactococcus lactis subsp. cremoris MG1363
Hydroxymethylglutaryl-CoA reductase
<400> 123
atgagaaaaa aattttatca aatgtcgcca caagagcgat tgaattcgtt aaacttatcc 60
gaaaagagtc aagaaatttt aagtgagatg gccctagata caactatttt ggacaatctc 120
atagaaaatc aaatttctga atttgaattg ccaatgggaa ttgcccaaaa ttttgttatt 180
aatggacaaa gttttctaat accaatggtt acagaggaac cgtcagtaat tgccgcagca 240
agtaatggag caaaaatagc tggaaatttt gtagctgaaa ttaaagaacg tttaatgcgt 300
ggtcaaattg tattttatga tgttaaaaat tcagataaga ttgcaaatga aatacttgaa 360
aagcaagaaa aaatctttga acaagccgaa ctttcttacc cttcaatcgt taaacgaggt 420
ggcggtttga gagaggtttc tagtcggatt ttctctagtc aaaagttttt atctgttgat 480
gtcaaggttg atgttaaaga cgccatgggt gcaaatatta ttaattcaat cttagaggga 540
attgctgaac tctttcggag gtggtttcct gatgaaaaaa ttctgtttag tattttatca 600
aattatgcta ctgaatcact agtgaaagtg acctgtgaaa ttccggttga acgcctttca 660
aaaaaagcag atggatatga aattggtcaa aaaattatgg ctgcttccca atattcaaaa 720
attgatcctt atcgtgctag cacccataac aaagggatta tgaacggtat caatgcggtc 780
attttagcta caggaaatga tactcgggcc atctcagctg ctattcatgc ttatgcggca 840
aaagatggag cctaccaagg gttagccaac tgggaacttc aagagaaaat gttagttggc 900
gaactcgaat ttcctttacc agtggcaact gtcggagggg gagtcaaggt tttgccaaaa 960
gcacaagcgg ccatggagat tctgggtatc agtgatgcta aggagctcgc aaaagttatt 1020
gctgcagtgg gactagctca aaacttagcc gctttacgtg ccctagtttc agaaggaatt 1080
caacagggac acatgagtct acaagctcgt tctttggctt tgagtgtagg agcacaagcg 1140
gatgaaatcg cgatattaag tcaacaattg cgcaaagaaa aagtgatgaa tcaagaagta 1200
gcccaaaatt tactaaaaaa tttgagaaaa taa 1233
<210> 124
<211> 803
<212> PRT
<213> Enterococcus faecalis mvaE
<400> 124
Leu Lys Thr Val Val Ile Ile Asp Ala Leu Arg Thr Pro Ile Gly Lys
1 5 10 15
Tyr Lys Gly Ser Leu Ser Gln Val Ser Ala Val Asp Leu Gly Thr His
20 25 30
Val Thr Thr Gln Leu Leu Lys Arg His Ser Thr Ile Ser Glu Glu Ile
35 40 45
Asp Gln Val Ile Phe Gly Asn Val Leu Gln Ala Gly Asn Gly Gln Asn
50 55 60
Pro Ala Arg Gln Ile Ala Ile Asn Ser Gly Leu Ser His Glu Ile Pro
65 70 75 80
Ala Met Thr Val Asn Glu Val Cys Gly Ser Gly Met Lys Ala Val Ile
85 90 95
Leu Ala Lys Gln Leu Ile Gln Leu Gly Glu Ala Glu Val Leu Ile Ala
100 105 110
Gly Gly Ile Glu Asn Met Ser Gln Ala Pro Lys Leu Gln Arg Phe Asn
115 120 125
Tyr Glu Thr Glu Ser Tyr Asp Ala Pro Phe Ser Ser Met Met Tyr Asp
130 135 140
Gly Leu Thr Asp Ala Phe Ser Gly Gln Ala Met Gly Leu Thr Ala Glu
145 150 155 160
Asn Val Ala Glu Lys Tyr His Val Thr Arg Glu Glu Gln Asp Gln Phe
165 170 175
Ser Val His Ser Gln Leu Lys Ala Ala Gln Ala Gln Ala Glu Gly Ile
180 185 190
Phe Ala Asp Glu Ile Ala Pro Leu Glu Val Ser Gly Thr Leu Val Glu
195 200 205
Lys Asp Glu Gly Ile Arg Pro Asn Ser Ser Val Glu Lys Leu Gly Thr
210 215 220
Leu Lys Thr Val Phe Lys Glu Asp Gly Thr Val Thr Ala Gly Asn Ala
225 230 235 240
Ser Thr Ile Asn Asp Gly Ala Ser Ala Leu Ile Ile Ala Ser Gln Glu
245 250 255
Tyr Ala Glu Ala His Gly Leu Pro Tyr Leu Ala Ile Ile Arg Asp Ser
260 265 270
Val Glu Val Gly Ile Asp Pro Ala Tyr Met Gly Ile Ser Pro Ile Lys
275 280 285
Ala Ile Gln Lys Leu Leu Ala Arg Asn Gln Leu Thr Thr Glu Glu Ile
290 295 300
Asp Leu Tyr Glu Ile Asn Glu Ala Phe Ala Ala Thr Ser Ile Val Val
305 310 315 320
Gln Arg Glu Leu Ala Leu Pro Glu Glu Lys Val Asn Ile Tyr Gly Gly
325 330 335
Gly Ile Ser Leu Gly His Ala Ile Gly Ala Thr Gly Ala Arg Leu Leu
340 345 350
Thr Ser Leu Ser Tyr Gln Leu Asn Gln Lys Glu Lys Lys Tyr Gly Val
355 360 365
Ala Ser Leu Cys Ile Gly Gly Gly Leu Gly Leu Ala Met Leu Leu Glu
370 375 380
Arg Pro Gln Gln Lys Lys Asn Ser Arg Phe Tyr Gln Met Ser Pro Glu
385 390 395 400
Glu Arg Leu Ala Ser Leu Leu Asn Glu Gly Gln Ile Ser Ala Asp Thr
405 410 415
Lys Lys Glu Phe Glu Asn Thr Ala Leu Ser Ser Gln Ile Ala Asn His
420 425 430
Met Ile Glu Asn Gln Ile Ser Glu Thr Glu Val Pro Met Gly Val Gly
435 440 445
Leu His Leu Thr Val Asp Glu Thr Asp Tyr Leu Val Pro Met Ala Thr
450 455 460
Glu Glu Pro Ser Val Ile Ala Ala Leu Ser Asn Gly Ala Lys Ile Ala
465 470 475 480
Gln Gly Phe Lys Thr Val Asn Gln Gln Arg Leu Met Arg Gly Gln Ile
485 490 495
Val Phe Tyr Asp Val Ala Asp Ala Glu Ser Leu Ile Asp Glu Leu Gln
500 505 510
Val Arg Glu Thr Glu Ile Phe Gln Gln Ala Glu Leu Ser Tyr Pro Ser
515 520 525
Ile Val Lys Arg Gly Gly Gly Leu Arg Asp Leu Gln Tyr Arg Ala Phe
530 535 540
Asp Glu Ser Phe Val Ser Val Asp Phe Leu Val Asp Val Lys Asp Ala
545 550 555 560
Met Gly Ala Asn Ile Val Asn Ala Met Leu Glu Gly Val Ala Glu Leu
565 570 575
Phe Arg Glu Trp Phe Ala Glu Gln Lys Ile Leu Phe Ser Ile Leu Ser
580 585 590
Asn Tyr Ala Thr Glu Ser Val Val Thr Met Lys Thr Ala Ile Pro Val
595 600 605
Ser Arg Leu Ser Lys Gly Ser Asn Gly Arg Glu Ile Ala Glu Lys Ile
610 615 620
Val Leu Ala Ser Arg Tyr Ala Ser Leu Asp Pro Tyr Arg Ala Val Thr
625 630 635 640
His Asn Lys Gly Ile Met Asn Gly Ile Glu Ala Val Val Leu Ala Thr
645 650 655
Gly Asn Asp Thr Arg Ala Val Ser Ala Ser Cys His Ala Phe Ala Val
660 665 670
Lys Glu Gly Arg Tyr Gln Gly Leu Thr Ser Trp Thr Leu Asp Gly Glu
675 680 685
Gln Leu Ile Gly Glu Ile Ser Val Pro Leu Ala Leu Ala Thr Val Gly
690 695 700
Gly Ala Thr Lys Val Leu Pro Lys Ser Gln Ala Ala Ala Asp Leu Leu
705 710 715 720
Ala Val Thr Asp Ala Lys Glu Leu Ser Arg Val Val Ala Ala Val Gly
725 730 735
Leu Ala Gln Asn Leu Ala Ala Leu Arg Ala Leu Val Ser Glu Gly Ile
740 745 750
Gln Lys Gly His Met Ala Leu Gln Ala Arg Ser Leu Ala Met Thr Val
755 760 765
Gly Ala Thr Gly Lys Glu Val Glu Ala Val Ala Gln Gln Leu Lys Arg
770 775 780
Gln Lys Thr Met Asn Gln Asp Arg Ala Leu Ala Ile Leu Asn Asp Leu
785 790 795 800
Arg Lys Gln
<210> 125
<211> 383
<212> PRT
<213> Enterococcus faecalis mvaS
<400> 125
Met Thr Ile Gly Ile Asp Lys Ile Ser Phe Phe Val Pro Pro Tyr Tyr
1 5 10 15
Ile Asp Met Thr Ala Leu Ala Glu Ala Arg Asn Val Asp Pro Gly Lys
20 25 30
Phe His Ile Gly Ile Gly Gln Asp Gln Met Ala Val Asn Pro Ile Ser
35 40 45
Gln Asp Ile Val Thr Phe Ala Ala Asn Ala Ala Glu Ala Ile Leu Thr
50 55 60
Lys Glu Asp Lys Glu Ala Ile Asp Met Val Ile Val Gly Thr Glu Ser
65 70 75 80
Ser Ile Asp Glu Ser Lys Ala Ala Ala Val Val Leu His Arg Leu Met
85 90 95
Gly Ile Gln Pro Phe Ala Arg Ser Phe Glu Ile Lys Glu Ala Cys Tyr
100 105 110
Gly Ala Thr Ala Gly Leu Gln Leu Ala Lys Asn His Val Ala Leu His
115 120 125
Pro Asp Lys Lys Val Leu Val Val Ala Ala Asp Ile Ala Lys Tyr Gly
130 135 140
Leu Asn Ser Gly Gly Glu Pro Thr Gln Gly Ala Gly Ala Val Ala Met
145 150 155 160
Leu Val Ala Ser Glu Pro Arg Ile Leu Ala Leu Lys Glu Asp Asn Val
165 170 175
Met Leu Thr Gln Asp Ile Tyr Asp Phe Trp Arg Pro Thr Gly His Pro
180 185 190
Tyr Pro Met Val Asp Gly Pro Leu Ser Asn Glu Thr Tyr Ile Gln Ser
195 200 205
Phe Ala Gln Val Trp Asp Glu His Lys Lys Arg Thr Gly Leu Asp Phe
210 215 220
Ala Asp Tyr Asp Ala Leu Ala Phe His Ile Pro Tyr Thr Lys Met Gly
225 230 235 240
Lys Lys Ala Leu Leu Ala Lys Ile Ser Asp Gln Thr Glu Ala Glu Gln
245 250 255
Glu Arg Ile Leu Ala Arg Tyr Glu Glu Ser Ile Ile Tyr Ser Arg Arg
260 265 270
Val Gly Asn Leu Tyr Thr Gly Ser Leu Tyr Leu Gly Leu Ile Ser Leu
275 280 285
Leu Glu Asn Ala Thr Thr Leu Thr Ala Gly Asn Gln Ile Gly Leu Phe
290 295 300
Ser Tyr Gly Ser Gly Ala Val Ala Glu Phe Phe Thr Gly Glu Leu Val
305 310 315 320
Ala Gly Tyr Gln Asn His Leu Gln Lys Glu Thr His Leu Ala Leu Leu
325 330 335
Asp Asn Arg Thr Glu Leu Ser Ile Ala Glu Tyr Glu Ala Met Phe Ala
340 345 350
Glu Thr Leu Asp Thr Asp Ile Asp Gln Thr Leu Glu Asp Glu Leu Lys
355 360 365
Tyr Ser Ile Ser Ala Ile Asn Asn Thr Val Arg Ser Tyr Arg Asn
370 375 380
<210> 126
<211> 292
<212> PRT
<213> Streptococcus pneumoniae mvaK1
<400> 126
Met Thr Lys Lys Val Gly Val Gly Gln Ala His Ser Lys Ile Ile Leu
1 5 10 15
Ile Gly Glu His Ala Val Val Tyr Gly Tyr Pro Ala Ile Ser Leu Pro
20 25 30
Leu Leu Glu Val Glu Val Thr Cys Lys Val Val Pro Ala Glu Ser Pro
35 40 45
Trp Arg Leu Tyr Glu Glu Asp Thr Leu Ser Met Ala Val Tyr Ala Ser
50 55 60
Leu Glu Tyr Leu Asn Ile Thr Glu Ala Cys Ile Arg Cys Glu Ile Asp
65 70 75 80
Ser Ala Ile Pro Glu Lys Arg Gly Met Gly Ser Ser Ala Ala Ile Ser
85 90 95
Ile Ala Ala Ile Arg Ala Val Phe Asp Tyr Tyr Gln Ala Asp Leu Pro
100 105 110
His Asp Val Leu Glu Ile Leu Val Asn Arg Ala Glu Met Ile Ala His
115 120 125
Met Asn Pro Ser Gly Leu Asp Ala Lys Thr Cys Leu Ser Asp Gln Pro
130 135 140
Ile Arg Phe Ile Lys Asn Val Gly Phe Thr Glu Leu Glu Met Asp Leu
145 150 155 160
Ser Ala Tyr Leu Val Ile Ala Asp Thr Gly Val Tyr Gly His Thr Arg
165 170 175
Glu Ala Ile Gln Val Val Gln Asn Lys Gly Lys Asp Ala Leu Pro Phe
180 185 190
Leu His Ala Leu Gly Glu Leu Thr Gln Gln Ala Glu Val Ala Ile Ser
195 200 205
Gln Lys Asp Ala Glu Gly Leu Gly Gln Ile Leu Ser Gln Ala His Leu
210 215 220
His Leu Lys Glu Ile Gly Val Ser Ser Pro Glu Ala Asp Phe Leu Val
225 230 235 240
Glu Thr Thr Leu Ser His Gly Ala Leu Gly Ala Lys Met Ser Gly Gly
245 250 255
Gly Leu Gly Gly Cys Ile Ile Ala Leu Val Thr Asn Leu Thr His Ala
260 265 270
Gln Glu Leu Ala Glu Arg Leu Glu Glu Lys Gly Ala Val Gln Thr Trp
275 280 285
Ile Glu Ser Leu
290
<210> 127
<211> 336
<212> PRT
<213> Streptococcus pneumoniae mvaK2
<400> 127
Met Ile Ala Val Lys Thr Cys Gly Lys Leu Tyr Trp Ala Gly Glu Tyr
1 5 10 15
Ala Ile Leu Glu Pro Gly Gln Leu Ala Leu Ile Lys Asp Ile Pro Ile
20 25 30
Tyr Met Arg Ala Glu Ile Ala Phe Ser Asp Ser Tyr Arg Ile Tyr Ser
35 40 45
Asp Met Phe Asp Phe Ala Val Asp Leu Arg Pro Asn Pro Asp Tyr Ser
50 55 60
Leu Ile Gln Glu Thr Ile Ala Leu Met Gly Asp Phe Leu Ala Val Arg
65 70 75 80
Gly Gln Asn Leu Arg Pro Phe Ser Leu Lys Ile Cys Gly Lys Met Glu
85 90 95
Arg Glu Gly Lys Lys Phe Gly Leu Gly Ser Ser Gly Ser Val Val Val
100 105 110
Leu Val Val Lys Ala Leu Leu Ala Leu Tyr Asn Leu Ser Val Asp Gln
115 120 125
Asn Leu Leu Phe Lys Leu Thr Ser Ala Val Leu Leu Lys Arg Gly Asp
130 135 140
Asn Gly Ser Met Gly Asp Leu Ala Cys Ile Val Ala Glu Asp Leu Val
145 150 155 160
Leu Tyr Gln Ser Phe Asp Arg Gln Lys Ala Ala Ala Trp Leu Glu Glu
165 170 175
Glu Asn Leu Ala Thr Val Leu Glu Arg Asp Trp Gly Phe Phe Ile Ser
180 185 190
Gln Val Lys Pro Thr Leu Glu Cys Asp Phe Leu Val Gly Trp Thr Lys
195 200 205
Glu Val Ala Val Ser Ser His Met Val Gln Gln Ile Lys Gln Asn Ile
210 215 220
Asn Gln Asn Phe Leu Ser Ser Ser Lys Glu Thr Val Val Ser Leu Val
225 230 235 240
Glu Ala Leu Glu Gln Gly Lys Ala Glu Lys Val Ile Glu Gln Val Glu
245 250 255
Val Ala Ser Lys Leu Leu Glu Gly Leu Ser Thr Asp Ile Tyr Thr Pro
260 265 270
Leu Leu Arg Gln Leu Lys Glu Ala Ser Gln Asp Leu Gln Ala Val Ala
275 280 285
Lys Ser Ser Gly Ala Gly Gly Gly Asp Cys Gly Ile Ala Leu Ser Phe
290 295 300
Asp Ala Gln Ser Ser Arg Asn Thr Leu Lys Asn Arg Trp Ala Asp Leu
305 310 315 320
Gly Ile Glu Leu Leu Tyr Gln Glu Arg Ile Gly His Asp Asp Lys Ser
325 330 335
<210> 128
<211> 317
<212> PRT
<213> Streptococcus pneumoniae mvaD
<400> 128
Met Asp Arg Glu Pro Val Thr Val Arg Ser Tyr Ala Asn Ile Ala Ile
1 5 10 15
Ile Lys Tyr Trp Gly Lys Lys Lys Glu Lys Glu Met Val Pro Ala Thr
20 25 30
Ser Ser Ile Ser Leu Thr Leu Glu Asn Met Tyr Thr Glu Thr Thr Leu
35 40 45
Ser Pro Leu Pro Ala Asn Val Thr Ala Asp Glu Phe Tyr Ile Asn Gly
50 55 60
Gln Leu Gln Asn Glu Val Glu His Ala Lys Met Ser Lys Ile Ile Asp
65 70 75 80
Arg Tyr Arg Pro Ala Gly Glu Gly Phe Val Arg Ile Asp Thr Gln Asn
85 90 95
Asn Met Pro Thr Ala Ala Gly Leu Ser Ser Ser Ser Ser Gly Leu Ser
100 105 110
Ala Leu Val Lys Ala Cys Asn Ala Tyr Phe Lys Leu Gly Leu Asp Arg
115 120 125
Ser Gln Leu Ala Gln Glu Ala Lys Phe Ala Ser Gly Ser Ser Ser Arg
130 135 140
Ser Phe Tyr Gly Pro Leu Gly Ala Trp Asp Lys Asp Ser Gly Glu Ile
145 150 155 160
Tyr Pro Val Glu Thr Asp Leu Lys Leu Ala Met Ile Met Leu Val Leu
165 170 175
Glu Asp Lys Lys Lys Pro Ile Ser Ser Arg Asp Gly Met Lys Leu Cys
180 185 190
Val Glu Thr Ser Thr Thr Phe Asp Asp Trp Val Arg Gln Ser Glu Lys
195 200 205
Asp Tyr Gln Asp Met Leu Ile Tyr Leu Lys Glu Asn Asp Phe Ala Lys
210 215 220
Ile Gly Glu Leu Thr Glu Lys Asn Ala Leu Ala Met His Ala Thr Thr
225 230 235 240
Lys Thr Ala Ser Pro Ala Phe Ser Tyr Leu Thr Asp Ala Ser Tyr Glu
245 250 255
Ala Met Ala Phe Val Arg Gln Leu Arg Glu Lys Gly Glu Ala Cys Tyr
260 265 270
Phe Thr Met Asp Ala Gly Pro Asn Val Lys Val Phe Cys Gln Glu Lys
275 280 285
Asp Leu Glu His Leu Ser Glu Ile Phe Gly Gln Arg Tyr Arg Leu Ile
290 295 300
Val Ser Lys Thr Lys Asp Leu Ser Gln Asp Asp Cys Cys
305 310 315
<210> 129
<211> 182
<212> PRT
<213> Escherichia coli idi
<400> 129
Met Gln Thr Glu His Val Ile Leu Leu Asn Ala Gln Gly Val Pro Thr
1 5 10 15
Gly Thr Leu Glu Lys Tyr Ala Ala His Thr Ala Asp Thr Arg Leu His
20 25 30
Leu Ala Phe Ser Ser Trp Leu Phe Asn Ala Lys Gly Gln Leu Leu Val
35 40 45
Thr Arg Arg Ala Leu Ser Lys Lys Ala Trp Pro Gly Val Trp Thr Asn
50 55 60
Ser Val Cys Gly His Pro Gln Leu Gly Glu Ser Asn Glu Asp Ala Val
65 70 75 80
Ile Arg Arg Cys Arg Tyr Glu Leu Gly Val Glu Ile Thr Pro Pro Glu
85 90 95
Ser Ile Tyr Pro Asp Phe Arg Tyr Arg Ala Thr Asp Pro Ser Gly Ile
100 105 110
Val Glu Asn Glu Val Cys Pro Val Phe Ala Ala Arg Thr Thr Ser Ala
115 120 125
Leu Gln Ile Asn Asp Asp Glu Val Met Asp Tyr Gln Trp Cys Asp Leu
130 135 140
Ala Asp Val Leu His Gly Ile Asp Ala Thr Pro Trp Ala Phe Ser Pro
145 150 155 160
Trp Met Val Met Gln Ala Thr Asn Arg Glu Ala Arg Lys Arg Leu Ser
165 170 175
Ala Phe Thr Gln Leu Lys
180
<210> 130
<211> 305
<212> PRT
<213> Haematococcus pluvialis ipiHp1
<400> 130
Met Leu Arg Ser Leu Leu Arg Gly Leu Thr His Ile Pro Arg Val Asn
1 5 10 15
Ser Ala Gln Gln Pro Ser Cys Ala His Ala Arg Leu Gln Phe Lys Leu
20 25 30
Arg Ser Met Gln Met Thr Leu Met Gln Pro Ser Ile Ser Ala Asn Leu
35 40 45
Ser Arg Ala Glu Asp Arg Thr Asp His Met Arg Gly Ala Ser Thr Trp
50 55 60
Ala Gly Gly Gln Ser Gln Asp Glu Leu Met Leu Lys Asp Glu Cys Ile
65 70 75 80
Leu Val Asp Val Glu Asp Asn Ile Thr Gly His Ala Ser Lys Leu Glu
85 90 95
Cys His Lys Phe Leu Pro His Gln Pro Ala Gly Leu Leu His Arg Ala
100 105 110
Phe Ser Val Phe Leu Phe Asp Asp Gln Gly Arg Leu Leu Leu Gln Gln
115 120 125
Arg Ala Arg Ser Lys Ile Thr Phe Pro Ser Val Trp Thr Asn Thr Cys
130 135 140
Cys Ser His Pro Leu His Gly Gln Thr Pro Asp Glu Val Asp Gln Leu
145 150 155 160
Ser Gln Val Ala Asp Gly Thr Val Pro Gly Ala Lys Ala Ala Ala Ile
165 170 175
Arg Lys Leu Glu His Glu Leu Gly Ile Pro Ala His Gln Leu Pro Ala
180 185 190
Ser Ala Phe Arg Phe Leu Thr Arg Leu His Tyr Cys Ala Ala Asp Val
195 200 205
Gln Pro Ala Ala Thr Gln Ser Ala Leu Trp Gly Glu His Glu Met Asp
210 215 220
Tyr Ile Leu Phe Ile Arg Ala Asn Val Thr Leu Ala Pro Asn Pro Asp
225 230 235 240
Glu Val Asp Glu Val Arg Tyr Val Thr Gln Glu Glu Leu Arg Gln Met
245 250 255
Met Gln Pro Asp Asn Gly Leu Gln Trp Ser Pro Trp Phe Arg Ile Ile
260 265 270
Ala Ala Arg Phe Leu Glu Arg Trp Trp Ala Asp Leu Asp Ala Ala Leu
275 280 285
Asn Thr Asp Lys His Glu Asp Trp Gly Thr Val His His Ile Asn Glu
290 295 300
Ala
305
<210> 131
<211> 309
<212> PRT
<213> pantoea agglomerans crtB
<400> 131
Met Ser Gln Pro Pro Leu Leu Asp His Ala Thr Gln Thr Met Ala Asn
1 5 10 15
Gly Ser Lys Ser Phe Ala Thr Ala Ala Lys Leu Phe Asp Pro Ala Thr
20 25 30
Arg Arg Ser Val Leu Met Leu Tyr Thr Trp Cys Arg His Cys Asp Asp
35 40 45
Val Ile Asp Asp Gln Thr His Gly Phe Ala Ser Glu Ala Ala Ala Glu
50 55 60
Glu Glu Ala Thr Gln Arg Leu Ala Arg Leu Arg Thr Leu Thr Leu Ala
65 70 75 80
Ala Phe Glu Gly Ala Glu Met Gln Asp Pro Ala Phe Ala Ala Phe Gln
85 90 95
Glu Val Ala Leu Thr His Gly Ile Thr Pro Arg Met Ala Leu Asp His
100 105 110
Leu Asp Gly Phe Ala Met Asp Val Ala Gln Thr Arg Tyr Val Thr Phe
115 120 125
Glu Asp Thr Leu Arg Tyr Cys Tyr His Val Ala Gly Val Val Gly Leu
130 135 140
Met Met Ala Arg Val Met Gly Val Arg Asp Glu Arg Val Leu Asp Arg
145 150 155 160
Ala Cys Asp Leu Gly Leu Ala Phe Gln Leu Thr Asn Ile Ala Arg Asp
165 170 175
Ile Ile Asp Asp Ala Ala Ile Asp Arg Cys Tyr Leu Pro Ala Glu Trp
180 185 190
Leu Gln Asp Ala Gly Leu Thr Pro Glu Asn Tyr Ala Ala Arg Glu Asn
195 200 205
Arg Ala Ala Leu Ala Arg Val Ala Glu Arg Leu Ile Asp Ala Ala Glu
210 215 220
Pro Tyr Tyr Ile Ser Ser Gln Ala Gly Leu His Asp Leu Pro Pro Arg
225 230 235 240
Cys Ala Trp Ala Ile Ala Thr Ala Arg Ser Val Tyr Arg Glu Ile Gly
245 250 255
Ile Lys Val Lys Ala Ala Gly Gly Ser Ala Trp Asp Arg Arg Gln His
260 265 270
Thr Ser Lys Gly Glu Lys Ile Ala Met Leu Met Ala Ala Pro Gly Gln
275 280 285
Val Ile Arg Ala Lys Thr Thr Arg Val Thr Pro Arg Pro Ala Gly Leu
290 295 300
Trp Gln Arg Pro Val
305
<210> 132
<211> 492
<212> PRT
<213> pantoea agglomerans crtI
<400> 132
Met Lys Lys Thr Val Val Ile Gly Ala Gly Phe Gly Gly Leu Ala Leu
1 5 10 15
Ala Ile Arg Leu Gln Ala Ala Gly Ile Pro Thr Val Leu Leu Glu Gln
20 25 30
Arg Asp Lys Pro Gly Gly Arg Ala Tyr Val Trp His Asp Gln Gly Phe
35 40 45
Thr Phe Asp Ala Gly Pro Thr Val Ile Thr Asp Pro Thr Ala Leu Glu
50 55 60
Ala Leu Phe Thr Leu Ala Gly Arg Arg Met Glu Asp Tyr Val Arg Leu
65 70 75 80
Leu Pro Val Lys Pro Phe Tyr Arg Leu Cys Trp Glu Ser Gly Lys Thr
85 90 95
Leu Asp Tyr Ala Asn Asp Ser Ala Glu Leu Glu Ala Gln Ile Thr Gln
100 105 110
Phe Asn Pro Arg Asp Val Glu Gly Tyr Arg Arg Phe Leu Ala Tyr Ser
115 120 125
Gln Ala Val Phe Gln Glu Gly Tyr Leu Arg Leu Gly Ser Val Pro Phe
130 135 140
Leu Ser Phe Arg Asp Met Leu Arg Ala Gly Pro Gln Leu Leu Lys Leu
145 150 155 160
Gln Ala Trp Gln Ser Val Tyr Gln Ser Val Ser Arg Phe Ile Glu Asp
165 170 175
Glu His Leu Arg Gln Ala Phe Ser Phe His Ser Leu Leu Val Gly Gly
180 185 190
Asn Pro Phe Thr Thr Ser Ser Ile Tyr Thr Leu Ile His Ala Leu Glu
195 200 205
Arg Glu Trp Gly Val Trp Phe Pro Glu Gly Gly Thr Gly Ala Leu Val
210 215 220
Asn Gly Met Val Lys Leu Phe Thr Asp Leu Gly Gly Glu Ile Glu Leu
225 230 235 240
Asn Ala Arg Val Glu Glu Leu Val Val Ala Asp Asn Arg Val Ser Gln
245 250 255
Val Arg Leu Ala Asp Gly Arg Ile Phe Asp Thr Asp Ala Val Ala Ser
260 265 270
Asn Ala Asp Val Val Asn Thr Tyr Lys Lys Leu Leu Gly His His Pro
275 280 285
Val Gly Gln Lys Arg Ala Ala Ala Leu Glu Arg Lys Ser Met Ser Asn
290 295 300
Ser Leu Phe Val Leu Tyr Phe Gly Leu Asn Gln Pro His Ser Gln Leu
305 310 315 320
Ala His His Thr Ile Cys Phe Gly Pro Arg Tyr Arg Glu Leu Ile Asp
325 330 335
Glu Ile Phe Thr Gly Ser Ala Leu Ala Asp Asp Phe Ser Leu Tyr Leu
340 345 350
His Ser Pro Cys Val Thr Asp Pro Ser Leu Ala Pro Pro Gly Cys Ala
355 360 365
Ser Phe Tyr Val Leu Ala Pro Val Pro His Leu Gly Asn Ala Pro Leu
370 375 380
Asp Trp Ala Gln Glu Gly Pro Lys Leu Arg Asp Arg Ile Phe Asp Tyr
385 390 395 400
Leu Glu Glu Arg Tyr Met Pro Gly Leu Arg Ser Gln Leu Val Thr Gln
405 410 415
Arg Ile Phe Thr Pro Ala Asp Phe His Asp Thr Leu Asp Ala His Leu
420 425 430
Gly Ser Ala Phe Ser Ile Glu Pro Leu Leu Thr Gln Ser Ala Trp Phe
435 440 445
Arg Pro His Asn Arg Asp Ser Asp Ile Ala Asn Leu Tyr Leu Val Gly
450 455 460
Ala Gly Thr His Pro Gly Ala Gly Ile Pro Gly Val Val Ala Ser Ala
465 470 475 480
Lys Ala Thr Ala Ser Leu Met Ile Glu Asp Leu Gln
485 490
<210> 133
<211> 382
<212> PRT
<213> pantoea ananatis crtY
<400> 133
Met Gln Pro His Tyr Asp Leu Ile Leu Val Gly Ala Gly Leu Ala Asn
1 5 10 15
Gly Leu Ile Ala Leu Arg Leu Gln Gln Gln Gln Pro Asp Met Arg Ile
20 25 30
Leu Leu Ile Asp Ala Ala Pro Gln Ala Gly Gly Asn His Thr Trp Ser
35 40 45
Phe His His Asp Asp Leu Thr Glu Ser Gln His Arg Trp Ile Ala Pro
50 55 60
Leu Val Val His His Trp Pro Asp Tyr Gln Val Arg Phe Pro Thr Arg
65 70 75 80
Arg Arg Lys Leu Asn Ser Gly Tyr Phe Cys Ile Thr Ser Gln Arg Phe
85 90 95
Ala Glu Val Leu Gln Arg Gln Phe Gly Pro His Leu Trp Met Asp Thr
100 105 110
Ala Val Ala Glu Val Asn Ala Glu Ser Val Arg Leu Lys Lys Gly Gln
115 120 125
Val Ile Gly Ala Arg Ala Val Ile Asp Gly Arg Gly Tyr Ala Ala Asn
130 135 140
Ser Ala Leu Ser Val Gly Phe Gln Ala Phe Ile Gly Gln Glu Trp Arg
145 150 155 160
Leu Ser His Pro His Gly Leu Ser Ser Pro Ile Ile Met Asp Ala Thr
165 170 175
Val Asp Gln Gln Asn Gly Tyr Arg Phe Val Tyr Ser Leu Pro Leu Ser
180 185 190
Pro Thr Arg Leu Leu Ile Glu Asp Thr His Tyr Ile Asp Asn Ala Thr
195 200 205
Leu Asp Pro Glu Cys Ala Arg Gln Asn Ile Cys Asp Tyr Ala Ala Gln
210 215 220
Gln Gly Trp Gln Leu Gln Thr Leu Leu Arg Glu Glu Gln Gly Ala Leu
225 230 235 240
Pro Ile Thr Leu Ser Gly Asn Ala Asp Ala Phe Trp Gln Gln Arg Pro
245 250 255
Leu Ala Cys Ser Gly Leu Arg Ala Gly Leu Phe His Pro Thr Thr Gly
260 265 270
Tyr Ser Leu Pro Leu Ala Val Ala Val Ala Asp Arg Leu Ser Ala Leu
275 280 285
Asp Val Phe Thr Ser Ala Ser Ile His His Ala Ile Thr His Phe Ala
290 295 300
Arg Glu Arg Trp Gln Gln Gln Gly Phe Phe Arg Met Leu Asn Arg Met
305 310 315 320
Leu Phe Leu Ala Gly Pro Ala Asp Ser Arg Trp Arg Val Met Gln Arg
325 330 335
Phe Tyr Gly Leu Pro Glu Asp Leu Ile Ala Arg Phe Tyr Ala Gly Lys
340 345 350
Leu Thr Leu Thr Asp Arg Leu Arg Ile Leu Ser Gly Lys Pro Pro Val
355 360 365
Pro Val Leu Ala Ala Leu Gln Ala Ile Met Thr Thr His Arg
370 375 380
<210> 134
<211> 275
<212> PRT
<213> uncultured marine bacterium 66A03 SR
<400> 134
Met Gly Leu Met Leu Ile Asp Trp Cys Ala Leu Ala Leu Val Val Phe
1 5 10 15
Ile Gly Leu Pro His Gly Ala Leu Asp Ala Ala Ile Ser Phe Ser Met
20 25 30
Ile Ser Ser Ala Lys Arg Ile Ala Arg Leu Ala Gly Ile Leu Leu Ile
35 40 45
Tyr Leu Leu Leu Ala Thr Ala Phe Phe Leu Ile Trp Tyr Gln Leu Pro
50 55 60
Ala Phe Ser Leu Leu Ile Phe Leu Leu Ile Ser Ile Ile His Phe Gly
65 70 75 80
Met Ala Asp Phe Asn Ala Ser Pro Ser Lys Leu Lys Trp Pro His Ile
85 90 95
Ile Ala His Gly Gly Val Val Thr Val Trp Leu Pro Leu Ile Gln Lys
100 105 110
Asn Glu Val Thr Lys Leu Phe Ser Ile Leu Thr Asn Gly Pro Thr Pro
115 120 125
Ile Leu Trp Asp Ile Leu Leu Ile Phe Phe Leu Cys Trp Ser Ile Gly
130 135 140
Val Cys Leu His Thr Tyr Glu Thr Leu Arg Ser Lys His Tyr Asn Ile
145 150 155 160
Ala Phe Glu Leu Ile Gly Leu Ile Phe Leu Ala Trp Tyr Ala Pro Pro
165 170 175
Leu Val Thr Phe Ala Thr Tyr Phe Cys Phe Ile His Ser Arg Arg His
180 185 190
Phe Ser Phe Val Trp Lys Gln Leu Gln His Met Ser Ser Lys Lys Met
195 200 205
Met Ile Gly Ser Ala Ile Ile Leu Ser Cys Thr Ser Trp Leu Ile Gly
210 215 220
Gly Gly Ile Tyr Phe Phe Leu Asn Ser Lys Met Ile Ala Ser Glu Ala
225 230 235 240
Ala Leu Gln Thr Val Phe Ile Gly Leu Ala Ala Leu Thr Val Pro His
245 250 255
Met Ile Leu Ile Asp Phe Ile Phe Arg Pro His Ser Ser Arg Ile Lys
260 265 270
Ile Lys Asn
275
<210> 135
<211> 269
<212> PRT
<213> Escherichia coli MG1655 YbbO
<400> 135
Met Thr His Lys Ala Thr Glu Ile Leu Thr Gly Lys Val Met Gln Lys
1 5 10 15
Ser Val Leu Ile Thr Gly Cys Ser Ser Gly Ile Gly Leu Glu Ser Ala
20 25 30
Leu Glu Leu Lys Arg Gln Gly Phe His Val Leu Ala Gly Cys Arg Lys
35 40 45
Pro Asp Asp Val Glu Arg Met Asn Ser Met Gly Phe Thr Gly Val Leu
50 55 60
Ile Asp Leu Asp Ser Pro Glu Ser Val Asp Arg Ala Ala Asp Glu Val
65 70 75 80
Ile Ala Leu Thr Asp Asn Cys Leu Tyr Gly Ile Phe Asn Asn Ala Gly
85 90 95
Phe Gly Met Tyr Gly Pro Leu Ser Thr Ile Ser Arg Ala Gln Met Glu
100 105 110
Gln Gln Phe Ser Ala Asn Phe Phe Gly Ala His Gln Leu Thr Met Arg
115 120 125
Leu Leu Pro Ala Met Leu Pro His Gly Glu Gly Arg Ile Val Met Thr
130 135 140
Ser Ser Val Met Gly Leu Ile Ser Thr Pro Gly Arg Gly Ala Tyr Ala
145 150 155 160
Ala Ser Lys Tyr Ala Leu Glu Ala Trp Ser Asp Ala Leu Arg Met Glu
165 170 175
Leu Arg His Ser Gly Ile Lys Val Ser Leu Ile Glu Pro Gly Pro Ile
180 185 190
Arg Thr Arg Phe Thr Asp Asn Val Asn Gln Thr Gln Ser Asp Lys Pro
195 200 205
Val Glu Asn Pro Gly Ile Ala Ala Arg Phe Thr Leu Gly Pro Glu Ala
210 215 220
Val Val Asp Lys Val Arg His Ala Phe Ile Ser Glu Lys Pro Lys Met
225 230 235 240
Arg Tyr Pro Val Thr Leu Val Thr Trp Ala Val Met Val Leu Lys Arg
245 250 255
Leu Leu Pro Gly Arg Val Met Asp Lys Ile Leu Gln Gly
260 265
<210> 136
<211> 620
<212> PRT
<213> Escherichia coli dxs
<400> 136
Met Ser Phe Asp Ile Ala Lys Tyr Pro Thr Leu Ala Leu Val Asp Ser
1 5 10 15
Thr Gln Glu Leu Arg Leu Leu Pro Lys Glu Ser Leu Pro Lys Leu Cys
20 25 30
Asp Glu Leu Arg Arg Tyr Leu Leu Asp Ser Val Ser Arg Ser Ser Gly
35 40 45
His Phe Ala Ser Gly Leu Gly Thr Val Glu Leu Thr Val Ala Leu His
50 55 60
Tyr Val Tyr Asn Thr Pro Phe Asp Gln Leu Ile Trp Asp Val Gly His
65 70 75 80
Gln Ala Tyr Pro His Lys Ile Leu Thr Gly Arg Arg Asp Lys Ile Gly
85 90 95
Thr Ile Arg Gln Lys Gly Gly Leu His Pro Phe Pro Trp Arg Gly Glu
100 105 110
Ser Glu Tyr Asp Val Leu Ser Val Gly His Ser Ser Thr Ser Ile Ser
115 120 125
Ala Gly Ile Gly Ile Ala Val Ala Ala Glu Lys Glu Gly Lys Asn Arg
130 135 140
Arg Thr Val Cys Val Ile Gly Asp Gly Ala Ile Thr Ala Gly Met Ala
145 150 155 160
Phe Glu Ala Met Asn His Ala Gly Asp Ile Arg Pro Asp Met Leu Val
165 170 175
Ile Leu Asn Asp Asn Glu Met Ser Ile Ser Glu Asn Val Gly Ala Leu
180 185 190
Asn Asn His Leu Ala Gln Leu Leu Ser Gly Lys Leu Tyr Ser Ser Leu
195 200 205
Arg Glu Gly Gly Lys Lys Val Phe Ser Gly Val Pro Pro Ile Lys Glu
210 215 220
Leu Leu Lys Arg Thr Glu Glu His Ile Lys Gly Met Val Val Pro Gly
225 230 235 240
Thr Leu Phe Glu Glu Leu Gly Phe Asn Tyr Ile Gly Pro Val Asp Gly
245 250 255
His Asp Val Leu Gly Leu Ile Thr Thr Leu Lys Asn Met Arg Asp Leu
260 265 270
Lys Gly Pro Gln Phe Leu His Ile Met Thr Lys Lys Gly Arg Gly Tyr
275 280 285
Glu Pro Ala Glu Lys Asp Pro Ile Thr Phe His Ala Val Pro Lys Phe
290 295 300
Asp Pro Ser Ser Gly Cys Leu Pro Lys Ser Ser Gly Gly Leu Pro Ser
305 310 315 320
Tyr Ser Lys Ile Phe Gly Asp Trp Leu Cys Glu Thr Ala Ala Lys Asp
325 330 335
Asn Lys Leu Met Ala Ile Thr Pro Ala Met Arg Glu Gly Ser Gly Met
340 345 350
Val Glu Phe Ser Arg Lys Phe Pro Asp Arg Tyr Phe Asp Val Ala Ile
355 360 365
Ala Glu Gln His Ala Val Thr Phe Ala Ala Gly Leu Ala Ile Gly Gly
370 375 380
Tyr Lys Pro Ile Val Ala Ile Tyr Ser Thr Phe Leu Gln Arg Ala Tyr
385 390 395 400
Asp Gln Val Leu His Asp Val Ala Ile Gln Lys Leu Pro Val Leu Phe
405 410 415
Ala Ile Asp Arg Ala Gly Ile Val Gly Ala Asp Gly Gln Thr His Gln
420 425 430
Gly Ala Phe Asp Leu Ser Tyr Leu Arg Cys Ile Pro Glu Met Val Ile
435 440 445
Met Thr Pro Ser Asp Glu Asn Glu Cys Arg Gln Met Leu Tyr Thr Gly
450 455 460
Tyr His Tyr Asn Asp Gly Pro Ser Ala Val Arg Tyr Pro Arg Gly Asn
465 470 475 480
Ala Val Gly Val Glu Leu Thr Pro Leu Glu Lys Leu Pro Ile Gly Lys
485 490 495
Gly Ile Val Lys Arg Arg Gly Glu Lys Leu Ala Ile Leu Asn Phe Gly
500 505 510
Thr Leu Met Pro Glu Ala Ala Lys Val Ala Glu Ser Leu Asn Ala Thr
515 520 525
Leu Val Asp Met Arg Phe Val Lys Pro Leu Asp Glu Ala Leu Ile Leu
530 535 540
Glu Met Ala Ala Ser His Glu Ala Leu Val Thr Val Glu Glu Asn Ala
545 550 555 560
Ile Met Gly Gly Ala Gly Ser Gly Val Asn Glu Val Leu Met Ala His
565 570 575
Arg Lys Pro Val Pro Val Leu Asn Ile Gly Leu Pro Asp Phe Phe Ile
580 585 590
Pro Gln Gly Thr Gln Glu Glu Met Arg Ala Glu Leu Gly Leu Asp Ala
595 600 605
Ala Gly Met Glu Ala Lys Ile Lys Ala Trp Leu Ala
610 615 620
<210> 137
<211> 398
<212> PRT
<213> Escherichia coli dxr
<400> 137
Met Lys Gln Leu Thr Ile Leu Gly Ser Thr Gly Ser Ile Gly Cys Ser
1 5 10 15
Thr Leu Asp Val Val Arg His Asn Pro Glu His Phe Arg Val Val Ala
20 25 30
Leu Val Ala Gly Lys Asn Val Thr Arg Met Val Glu Gln Cys Leu Glu
35 40 45
Phe Ser Pro Arg Tyr Ala Val Met Asp Asp Glu Ala Ser Ala Lys Leu
50 55 60
Leu Lys Thr Met Leu Gln Gln Gln Gly Ser Arg Thr Glu Val Leu Ser
65 70 75 80
Gly Gln Gln Ala Ala Cys Asp Met Ala Ala Leu Glu Asp Val Asp Gln
85 90 95
Val Met Ala Ala Ile Val Gly Ala Ala Gly Leu Leu Pro Thr Leu Ala
100 105 110
Ala Ile Arg Ala Gly Lys Thr Ile Leu Leu Ala Asn Lys Glu Ser Leu
115 120 125
Val Thr Cys Gly Arg Leu Phe Met Asp Ala Val Lys Gln Ser Lys Ala
130 135 140
Gln Leu Leu Pro Val Asp Ser Glu His Asn Ala Ile Phe Gln Ser Leu
145 150 155 160
Pro Gln Pro Ile Gln His Asn Leu Gly Tyr Ala Asp Leu Glu Gln Asn
165 170 175
Gly Val Val Ser Ile Leu Leu Thr Gly Ser Gly Gly Pro Phe Arg Glu
180 185 190
Thr Pro Leu Arg Asp Leu Ala Thr Met Thr Pro Asp Gln Ala Cys Arg
195 200 205
His Pro Asn Trp Ser Met Gly Arg Lys Ile Ser Val Asp Ser Ala Thr
210 215 220
Met Met Asn Lys Gly Leu Glu Tyr Ile Glu Ala Arg Trp Leu Phe Asn
225 230 235 240
Ala Ser Ala Ser Gln Met Glu Val Leu Ile His Pro Gln Ser Val Ile
245 250 255
His Ser Met Val Arg Tyr Gln Asp Gly Ser Val Leu Ala Gln Leu Gly
260 265 270
Glu Pro Asp Met Arg Thr Pro Ile Ala His Thr Met Ala Trp Pro Asn
275 280 285
Arg Val Asn Ser Gly Val Lys Pro Leu Asp Phe Cys Lys Leu Ser Ala
290 295 300
Leu Thr Phe Ala Ala Pro Asp Tyr Asp Arg Tyr Pro Cys Leu Lys Leu
305 310 315 320
Ala Met Glu Ala Phe Glu Gln Gly Gln Ala Ala Thr Thr Ala Leu Asn
325 330 335
Ala Ala Asn Glu Ile Thr Val Ala Ala Phe Leu Ala Gln Gln Ile Arg
340 345 350
Phe Thr Asp Ile Ala Ala Leu Asn Leu Ser Val Leu Glu Lys Met Asp
355 360 365
Met Arg Glu Pro Gln Cys Val Asp Asp Val Leu Ser Val Asp Ala Asn
370 375 380
Ala Arg Glu Val Ala Arg Lys Glu Val Met Arg Leu Ala Ser
385 390 395
<210> 138
<211> 299
<212> PRT
<213> Escherichia coli ispA
<400> 138
Met Asp Phe Pro Gln Gln Leu Glu Ala Cys Val Lys Gln Ala Asn Gln
1 5 10 15
Ala Leu Ser Arg Phe Ile Ala Pro Leu Pro Phe Gln Asn Thr Pro Val
20 25 30
Val Glu Thr Met Gln Tyr Gly Ala Leu Leu Gly Gly Lys Arg Leu Arg
35 40 45
Pro Phe Leu Val Tyr Ala Thr Gly His Met Phe Gly Val Ser Thr Asn
50 55 60
Thr Leu Asp Ala Pro Ala Ala Ala Val Glu Cys Ile His Ala Tyr Ser
65 70 75 80
Leu Ile His Asp Asp Leu Pro Ala Met Asp Asp Asp Asp Leu Arg Arg
85 90 95
Gly Leu Pro Thr Cys His Val Lys Phe Gly Glu Ala Asn Ala Ile Leu
100 105 110
Ala Gly Asp Ala Leu Gln Thr Leu Ala Phe Ser Ile Leu Ser Asp Ala
115 120 125
Asp Met Pro Glu Val Ser Asp Arg Asp Arg Ile Ser Met Ile Ser Glu
130 135 140
Leu Ala Ser Ala Ser Gly Ile Ala Gly Met Cys Gly Gly Gln Ala Leu
145 150 155 160
Asp Leu Asp Ala Glu Gly Lys His Val Pro Leu Asp Ala Leu Glu Arg
165 170 175
Ile His Arg His Lys Thr Gly Ala Leu Ile Arg Ala Ala Val Arg Leu
180 185 190
Gly Ala Leu Ser Ala Gly Asp Lys Gly Arg Arg Ala Leu Pro Val Leu
195 200 205
Asp Lys Tyr Ala Glu Ser Ile Gly Leu Ala Phe Gln Val Gln Asp Asp
210 215 220
Ile Leu Asp Val Val Gly Asp Thr Ala Thr Leu Gly Lys Arg Gln Gly
225 230 235 240
Ala Asp Gln Gln Leu Gly Lys Ser Thr Tyr Pro Ala Leu Leu Gly Leu
245 250 255
Glu Gln Ala Arg Lys Lys Ala Arg Asp Leu Ile Asp Asp Ala Arg Gln
260 265 270
Ser Leu Lys Gln Leu Ala Glu Gln Ser Leu Asp Thr Ser Ala Leu Glu
275 280 285
Ala Leu Ala Asp Tyr Ile Ile Gln Arg Asn Lys
290 295
<210> 139
<211> 551
<212> PRT
<213> Clausena lansium STS
<400> 139
Met Ser Thr Gln Gln Val Ser Ser Glu Asn Ile Val Arg Asn Ala Ala
1 5 10 15
Asn Phe His Pro Asn Ile Trp Gly Asn His Phe Leu Thr Cys Pro Ser
20 25 30
Gln Thr Ile Asp Ser Trp Thr Gln Gln His His Lys Glu Leu Lys Glu
35 40 45
Glu Val Arg Lys Met Met Val Ser Asp Ala Asn Lys Pro Ala Gln Arg
50 55 60
Leu Arg Leu Ile Asp Thr Val Gln Arg Leu Gly Val Ala Tyr His Phe
65 70 75 80
Glu Lys Glu Ile Asp Asp Ala Leu Glu Lys Ile Gly His Asp Pro Phe
85 90 95
Asp Asp Lys Asp Asp Leu Tyr Ile Val Ser Leu Cys Phe Arg Leu Leu
100 105 110
Arg Gln His Gly Ile Lys Ile Ser Cys Asp Val Phe Glu Lys Phe Lys
115 120 125
Asp Asp Asp Gly Lys Phe Lys Ala Ser Leu Met Asn Asp Val Gln Gly
130 135 140
Met Leu Ser Leu Tyr Glu Ala Ala His Leu Ala Ile His Gly Glu Asp
145 150 155 160
Ile Leu Asp Glu Ala Ile Val Phe Thr Thr Thr His Leu Lys Ser Thr
165 170 175
Val Ser Asn Ser Pro Val Asn Ser Thr Phe Ala Glu Gln Ile Arg His
180 185 190
Ser Leu Arg Val Pro Leu Arg Lys Ala Val Pro Arg Leu Glu Ser Arg
195 200 205
Tyr Phe Leu Asp Ile Tyr Ser Arg Asp Asp Leu His Asp Lys Thr Leu
210 215 220
Leu Asn Phe Ala Lys Leu Asp Phe Asn Ile Leu Gln Ala Met His Gln
225 230 235 240
Lys Glu Ala Ser Glu Met Thr Arg Trp Trp Arg Asp Phe Asp Phe Leu
245 250 255
Lys Lys Leu Pro Tyr Ile Arg Asp Arg Val Val Glu Leu Tyr Phe Trp
260 265 270
Ile Leu Val Gly Val Ser Tyr Gln Pro Lys Phe Ser Thr Gly Arg Ile
275 280 285
Phe Leu Ser Lys Ile Ile Cys Leu Glu Thr Leu Val Asp Asp Thr Phe
290 295 300
Asp Ala Tyr Gly Thr Phe Asp Glu Leu Ala Ile Phe Thr Glu Ala Val
305 310 315 320
Thr Arg Trp Asp Leu Gly His Arg Asp Ala Leu Pro Glu Tyr Met Lys
325 330 335
Phe Ile Phe Lys Thr Leu Ile Asp Val Tyr Ser Glu Ala Glu Gln Glu
340 345 350
Leu Ala Lys Glu Gly Arg Ser Tyr Ser Ile His Tyr Ala Ile Arg Ser
355 360 365
Phe Gln Glu Leu Val Met Lys Tyr Phe Cys Glu Ala Lys Trp Leu Asn
370 375 380
Lys Gly Tyr Val Pro Ser Leu Asp Asp Tyr Lys Ser Val Ser Leu Arg
385 390 395 400
Ser Ile Gly Phe Leu Pro Ile Ala Val Ala Ser Phe Val Phe Met Gly
405 410 415
Asp Ile Ala Thr Lys Glu Val Phe Glu Trp Glu Met Asn Asn Pro Lys
420 425 430
Ile Ile Ile Ala Ala Glu Thr Ile Phe Arg Phe Leu Asp Asp Ile Ala
435 440 445
Gly His Arg Phe Glu Gln Lys Arg Glu His Ser Pro Ser Ala Ile Glu
450 455 460
Cys Tyr Lys Asn Gln His Gly Val Ser Glu Glu Glu Ala Val Lys Ala
465 470 475 480
Leu Ser Leu Glu Val Ala Asn Ser Trp Lys Asp Ile Asn Glu Glu Leu
485 490 495
Leu Leu Asn Pro Met Ala Ile Pro Leu Pro Leu Leu Gln Val Ile Leu
500 505 510
Asp Leu Ser Arg Ser Ala Asp Phe Met Tyr Gly Asn Ala Gln Asp Arg
515 520 525
Phe Thr His Ser Thr Met Met Lys Asp Gln Val Asp Leu Val Leu Lys
530 535 540
Asp Pro Val Lys Leu Asp Asp
545 550
<210> 140
<211> 572
<212> PRT
<213> Artificial Sequence
<220>
<223> Matricaria recutita MrBBS
<400> 140
Met Ser Thr Leu Ser Val Ser Thr Pro Ser Phe Ser Ser Ser Pro Leu
1 5 10 15
Ser Ser Val Asn Lys Asn Ser Thr Lys Gln His Val Thr Arg Asn Ser
20 25 30
Val Ile Phe His Asp Ser Ile Trp Gly Asp Gln Phe Leu Glu Tyr Lys
35 40 45
Glu Lys Phe Asn Val Ala Thr Glu Lys Gln Leu Ile Glu Glu Leu Lys
50 55 60
Glu Glu Val Arg Asn Glu Leu Met Ile Arg Ala Cys Asn Glu Ala Ser
65 70 75 80
Arg Tyr Ile Lys Leu Ile Gln Leu Ile Asp Val Val Glu Arg Leu Gly
85 90 95
Leu Ala Tyr His Phe Glu Lys Glu Ile Glu Glu Ser Leu Gln His Ile
100 105 110
Tyr Val Thr Tyr Gly His Lys Trp Thr Asn Tyr Asn Asn Ile Glu Ser
115 120 125
Leu Ser Leu Trp Phe Arg Leu Leu Arg Gln Asn Gly Phe Asn Val Ser
130 135 140
Ser Asp Ile Phe Glu Asn His Ile Asp Glu Lys Gly Asn Phe Gln Glu
145 150 155 160
Ser Leu Cys Asn Asp Pro Gln Gly Met Leu Ala Leu Tyr Glu Ala Ala
165 170 175
Tyr Met Arg Val Glu Gly Glu Ile Ile Leu Asp Lys Ala Leu Glu Phe
180 185 190
Thr Lys Leu His Leu Gly Ile Ile Ser Asn Asp Pro Ser Cys Asp Ser
195 200 205
Ser Leu Arg Thr Glu Ile Lys Gln Ala Leu Lys Gln Pro Leu Arg Arg
210 215 220
Arg Leu Pro Arg Leu Glu Ala Val Arg Tyr Ile Ala Ile Tyr Gln Gln
225 230 235 240
Lys Ala Ser His Ser Glu Val Leu Leu Lys Leu Ala Lys Leu Asp Phe
245 250 255
Asn Val Leu Gln Glu Met His Lys Asp Glu Leu Ser Gln Ile Cys Lys
260 265 270
Trp Trp Lys Asp Leu Asp Ile Arg Asn Lys Leu Pro Tyr Val Arg Asp
275 280 285
Arg Leu Ile Glu Gly Tyr Phe Trp Ile Leu Gly Ile Tyr Phe Glu Pro
290 295 300
Gln His Ser Arg Thr Arg Met Phe Leu Met Lys Thr Cys Met Trp Leu
305 310 315 320
Ile Val Leu Asp Asp Thr Phe Asp Asn Tyr Gly Thr Tyr Glu Glu Leu
325 330 335
Glu Ile Phe Thr Gln Ala Val Glu Arg Trp Ser Ile Thr Cys Leu Asp
340 345 350
Glu Leu Pro Glu Tyr Met Lys Leu Ile Tyr His Glu Gln Phe Arg Val
355 360 365
His Gln Glu Met Glu Glu Ser Leu Glu Lys Glu Gly Lys Ala Tyr Gln
370 375 380
Ile His Tyr Ile Lys Glu Met Ala Lys Glu Gly Thr Arg Ser Leu Leu
385 390 395 400
Leu Glu Ala Lys Trp Leu Lys Glu Gly Tyr Met Pro Thr Leu Asp Glu
405 410 415
Tyr Leu Ser Asn Ser Leu Val Thr Cys Gly Tyr Ala Leu Met Thr Ala
420 425 430
Arg Ser Tyr Val Ala Arg Asp Asp Gly Ile Val Thr Glu Asp Ala Phe
435 440 445
Lys Trp Val Ala Thr His Pro Pro Ile Val Lys Ala Ala Cys Lys Ile
450 455 460
Leu Arg Leu Met Asp Asp Ile Ala Thr His Lys Glu Glu Gln Glu Arg
465 470 475 480
Gly His Ile Ala Ser Ser Ile Glu Cys Tyr Arg Lys Glu Thr Gly Ala
485 490 495
Ser Glu Glu Glu Ala Cys Met Asp Phe Leu Lys Gln Val Glu Asp Gly
500 505 510
Trp Lys Val Ile Asn Gln Glu Ser Leu Met Pro Thr Asp Val Pro Phe
515 520 525
Pro Leu Leu Ile Pro Ala Ile Asn Leu Ala Arg Val Ser Asp Thr Leu
530 535 540
Tyr Lys Asp Asn Asp Gly Tyr Asn His Ala Asp Lys Glu Val Ile Gly
545 550 555 560
Tyr Ile Lys Ser Leu Phe Val His Pro Met Ile Val
565 570
<210> 141
<211> 391
<212> PRT
<213> Artificial Sequence
<220>
<223> EGFP
<400> 141
Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu
1 5 10 15
Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly
20 25 30
Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile
35 40 45
Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr
50 55 60
Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys
65 70 75 80
Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu
85 90 95
Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu
100 105 110
Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly
115 120 125
Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr
130 135 140
Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn
145 150 155 160
Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser
165 170 175
Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly
180 185 190
Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu
195 200 205
Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe
210 215 220
Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Gly
225 230 235 240
Tyr Pro Ala Gly Trp Arg Pro Leu Glu Met Val Glu Arg Lys Lys Leu
245 250 255
Gly Val Leu Gln Pro Thr Gln Gln Cys Arg Leu Thr Val His Gln Ser
260 265 270
Lys Ile Met Val Asp Ser Pro Lys Ala Leu Lys Arg Lys Ala Phe Gly
275 280 285
Asp Glu Asn Phe Ser Pro Val Pro Thr Leu Ser Arg Lys Ser Ile Phe
290 295 300
Glu Asp Pro Pro Thr Pro Ala Ser Lys Arg Ser Lys Leu Ser Asp Lys
305 310 315 320
Val Asp Ser Ala Ile Gln Thr Asp Asn Leu Cys Cys Gly Asn Gly Gly
325 330 335
Thr Glu Ile Ser Arg Gly Ser Ser Ser Ser Lys Ser Ser Ser Glu Ala
340 345 350
Ser Thr Lys Ala Cys Ser Gln Lys Ser Gln Ser Gln Ser Val Leu Asp
355 360 365
Met Leu Thr Ser Ala Glu Pro Asn Glu Gln Tyr Trp Gln Met Met Ala
370 375 380
Ala Glu Arg Lys Ala Ala Leu
385 390
<210> 142
<211> 410
<212> PRT
<213> Lactococcus lactis subsp. cremoris NZ9000 mvaA
<400> 142
Met Arg Lys Lys Phe Tyr Gln Met Ser Pro Gln Glu Arg Leu Asn Ser
1 5 10 15
Leu Asn Leu Ser Glu Lys Ser Gln Glu Ile Leu Ser Glu Met Ala Leu
20 25 30
Asp Thr Thr Ile Leu Asp Asn Leu Ile Glu Asn Gln Ile Ser Glu Phe
35 40 45
Glu Leu Pro Met Gly Ile Ala Gln Asn Phe Val Ile Asn Gly Gln Ser
50 55 60
Phe Leu Ile Pro Met Val Thr Glu Glu Pro Ser Val Ile Ala Ala Ala
65 70 75 80
Ser Asn Gly Ala Lys Ile Ala Gly Asn Phe Val Ala Glu Ile Lys Glu
85 90 95
Arg Leu Met Arg Gly Gln Ile Val Phe Tyr Asp Val Lys Asn Ser Asp
100 105 110
Lys Ile Ala Asn Glu Ile Leu Glu Lys Gln Glu Lys Ile Phe Glu Gln
115 120 125
Ala Glu Leu Ser Tyr Pro Ser Ile Val Lys Arg Gly Gly Gly Leu Arg
130 135 140
Glu Val Ser Ser Arg Ile Phe Ser Ser Gln Lys Phe Leu Ser Val Asp
145 150 155 160
Val Lys Val Asp Val Lys Asp Ala Met Gly Ala Asn Ile Ile Asn Ser
165 170 175
Ile Leu Glu Gly Ile Ala Glu Leu Phe Arg Arg Trp Phe Pro Asp Glu
180 185 190
Lys Ile Leu Phe Ser Ile Leu Ser Asn Tyr Ala Thr Glu Ser Leu Val
195 200 205
Lys Val Thr Cys Glu Ile Pro Val Glu Arg Leu Ser Lys Lys Ala Asp
210 215 220
Gly Tyr Glu Ile Gly Gln Lys Ile Met Ala Ala Ser Gln Tyr Ser Lys
225 230 235 240
Ile Asp Pro Tyr Arg Ala Ser Thr His Asn Lys Gly Ile Met Asn Gly
245 250 255
Ile Asn Ala Val Ile Leu Ala Thr Gly Asn Asp Thr Arg Ala Ile Ser
260 265 270
Ala Ala Ile His Ala Tyr Ala Ala Lys Asp Gly Ala Tyr Gln Gly Leu
275 280 285
Ala Asn Trp Glu Leu Gln Glu Lys Met Leu Val Gly Glu Leu Glu Phe
290 295 300
Pro Leu Pro Val Ala Thr Val Gly Gly Gly Val Lys Val Leu Pro Lys
305 310 315 320
Ala Gln Ala Ala Met Glu Ile Leu Gly Ile Ser Asp Ala Lys Glu Leu
325 330 335
Ala Lys Val Ile Ala Ala Val Gly Leu Ala Gln Asn Leu Ala Ala Leu
340 345 350
Arg Ala Leu Val Ser Glu Gly Ile Gln Gln Gly His Met Ser Leu Gln
355 360 365
Ala Arg Ser Leu Ala Leu Ser Val Gly Ala Gln Ala Asp Glu Ile Ala
370 375 380
Ile Leu Ser Gln Gln Leu Arg Lys Glu Lys Val Met Asn Gln Glu Val
385 390 395 400
Ala Gln Asn Leu Leu Lys Asn Leu Arg Lys
405 410
<210> 143
<211> 410
<212> PRT
<213> Artificial Sequence
<220>
<223> Lactococcus lactis subsp. cremoris MG1363
Hydroxymethylglutaryl-CoA reductase
<400> 143
Met Arg Lys Lys Phe Tyr Gln Met Ser Pro Gln Glu Arg Leu Asn Ser
1 5 10 15
Leu Asn Leu Ser Glu Lys Ser Gln Glu Ile Leu Ser Glu Met Ala Leu
20 25 30
Asp Thr Thr Ile Leu Asp Asn Leu Ile Glu Asn Gln Ile Ser Glu Phe
35 40 45
Glu Leu Pro Met Gly Ile Ala Gln Asn Phe Val Ile Asn Gly Gln Ser
50 55 60
Phe Leu Ile Pro Met Val Thr Glu Glu Pro Ser Val Ile Ala Ala Ala
65 70 75 80
Ser Asn Gly Ala Lys Ile Ala Gly Asn Phe Val Ala Glu Ile Lys Glu
85 90 95
Arg Leu Met Arg Gly Gln Ile Val Phe Tyr Asp Val Lys Asn Ser Asp
100 105 110
Lys Ile Ala Asn Glu Ile Leu Glu Lys Gln Glu Lys Ile Phe Glu Gln
115 120 125
Ala Glu Leu Ser Tyr Pro Ser Ile Val Lys Arg Gly Gly Gly Leu Arg
130 135 140
Glu Val Ser Ser Arg Ile Phe Ser Ser Gln Lys Phe Leu Ser Val Asp
145 150 155 160
Val Lys Val Asp Val Lys Asp Ala Met Gly Ala Asn Ile Ile Asn Ser
165 170 175
Ile Leu Glu Gly Ile Ala Glu Leu Phe Arg Arg Trp Phe Pro Asp Glu
180 185 190
Lys Ile Leu Phe Ser Ile Leu Ser Asn Tyr Ala Thr Glu Ser Leu Val
195 200 205
Lys Val Thr Cys Glu Ile Pro Val Glu Arg Leu Ser Lys Lys Ala Asp
210 215 220
Gly Tyr Glu Ile Gly Gln Lys Ile Met Ala Ala Ser Gln Tyr Ser Lys
225 230 235 240
Ile Asp Pro Tyr Arg Ala Ser Thr His Asn Lys Gly Ile Met Asn Gly
245 250 255
Ile Asn Ala Val Ile Leu Ala Thr Gly Asn Asp Thr Arg Ala Ile Ser
260 265 270
Ala Ala Ile His Ala Tyr Ala Ala Lys Asp Gly Ala Tyr Gln Gly Leu
275 280 285
Ala Asn Trp Glu Leu Gln Glu Lys Met Leu Val Gly Glu Leu Glu Phe
290 295 300
Pro Leu Pro Val Ala Thr Val Gly Gly Gly Val Lys Val Leu Pro Lys
305 310 315 320
Ala Gln Ala Ala Met Glu Ile Leu Gly Ile Ser Asp Ala Lys Glu Leu
325 330 335
Ala Lys Val Ile Ala Ala Val Gly Leu Ala Gln Asn Leu Ala Ala Leu
340 345 350
Arg Ala Leu Val Ser Glu Gly Ile Gln Gln Gly His Met Ser Leu Gln
355 360 365
Ala Arg Ser Leu Ala Leu Ser Val Gly Ala Gln Ala Asp Glu Ile Ala
370 375 380
Ile Leu Ser Gln Gln Leu Arg Lys Glu Lys Val Met Asn Gln Glu Val
385 390 395 400
Ala Gln Asn Leu Leu Lys Asn Leu Arg Lys
405 410
Claims (17)
- 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로의 효소를 코딩하는 유전자 중 적어도 하나가 감쇄 또는 결손되고,
목적 산물 합성 경로의 효소를 코딩하는 유전자; 및 형질전환 마커 유전자로서 상기 감쇄 또는 결손된 유전자와 동일 효소를 코딩하는 유전자 또는 그 보완 유전자가 도입된 플라스미드로 형질전환된 것이고,
상기 목적 산물 합성 경로의 효소를 코딩하는 유전자는 이소프레노이드, 산탈렌, 비사볼롤 및 레티놀 합성 경로 이루어진 군에서 선택된 경로의 효소를 코딩하는 유전자인 형질전환된 비인간 생물체.
- 청구항 1에 있어서, 상기 생물체는 내재적으로 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로를 갖는 것인 생물체.
- 청구항 1에 있어서, 상기 합성 경로는 MEP 경로 또는 MVA 경로인 생물체.
- 청구항 1에 있어서, 상기 감쇄 또는 결손되는 유전자는 MEP 경로 상의 효소를 코딩하는 유전자인 생물체.
- 청구항 1에 있어서, 상기 감쇄 또는 결손되는 유전자는 MEP 경로 상의 효소를 코딩하는 유전자이고, 상기 보완 유전자는 MVA 경로 상의 효소 중 적어도 하나를 코딩하는 유전자인 생물체.
- 청구항 1에 있어서, 상기 감쇄 또는 결손되는 유전자는 MVA 경로 상의 효소를 코딩하는 유전자인 생물체.
- 삭제
- 형질전환 대상 생물체의 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로의 효소를 코딩하는 유전자 중 적어도 하나를 감쇄 또는 결손시키는 단계; 및
상기 생물체를 목적 산물 합성 경로의 효소를 코딩하는 유전자; 및 형질전환 마커 유전자로서 감쇄 또는 결손된 유전자와 동일 효소를 코딩하는 유전자 또는 이의 보완 유전자가 도입된 플라스미드로 형질전환시키는 단계;를 포함하는 비인간 생물체의 형질 전환 방법.
- 청구항 8에 있어서, 상기 생물체는 내재적으로 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로를 갖는 것인 생물체의 형질 전환 방법.
- 청구항 8에 있어서, 상기 형질 전환은 항생제 없이 수행되는 것인 생물체의 형질 전환 방법.
- 청구항 8에 있어서, 상기 합성 경로는 MEP 경로 또는 MVA 경로인 생물체의 형질 전환 방법.
- 청구항 8에 있어서, 상기 감쇄 또는 결손되는 유전자는 MEP 경로 상의 효소를 코딩하는 유전자인 생물체의 형질 전환 방법.
- 청구항 8에 있어서, 상기 감쇄 또는 결손되는 유전자는 MEP 경로 상의 효소를 코딩하는 유전자이고, 상기 보완 유전자는 MVA 경로 상의 효소 중 적어도 하나를 코딩하는 유전자인 생물체의 형질 전환 방법.
- 청구항 8에 있어서, 상기 감쇄 또는 결손되는 유전자는 MVA 경로 상의 효소 중 적어도 하나를 코딩하는 유전자인 미생물의 형질 전환 방법.
- 청구항 8에 있어서, 상기 목적 산물 합성 경로의 효소를 코딩하는 유전자는 이소프레노이드, 산탈렌, 비사볼롤 및 레티놀 합성 경로 이루어진 군에서 선택된 경로의 효소를 코딩하는 유전자인 미생물의 형질 전환 방법.
- 청구항 8에 있어서, 상기 감쇄 또는 결손시키는 단계에서 적어도 2개의 유전자를 감쇄 또는 결손시키고, 상기 형질전환시키는 단계에서 상기 생물체를 상기 감쇄 또는 결손된 유전자와 동일한 효소를 코딩하는 유전자를 각각 포함하는 적어도 2개의 플라스미드로 형질전환시키는 미생물의 형질 전환 방법.
- 청구항 1 내지 6 중 어느 한 항의 생물체를 목적 산물 생산에 필요한 기질이 포함된 배지 상에서 배양하는 단계를 포함하는 목적 산물의 생산 방법.
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| KR100704776B1 (ko) * | 2004-11-06 | 2007-04-10 | 경상대학교산학협력단 | 내재적 비메발론산 경로는 불활성화되어 있고, 외래메발론산 경로에 관련되는 모든 효소를 코딩하는 ｄｎａ로형질전환된 숙주세포를 이용한 메발론산 경로 및비메발론산 경로 중 어느 하나 또는 모두를 저해하는물질을 탐색하는 방법 |
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| KR100971508B1 (ko) | 2008-01-14 | 2010-07-21 | 경상대학교산학협력단 | 이소프레노이드 생산성이 향상된 에세리키아 속 미생물 및그를 이용하여 이소프레노이드를 생산하는 방법 |
| KR101190255B1 (ko) | 2010-05-11 | 2012-10-12 | 대한민국(농촌진흥청장) | 두 가지 제초제에 대하여 저항성을 가지는 항생제 마커프리 형질전환 크리핑 벤트그라스 식물체 |
| WO2013019051A2 (ko) * | 2011-07-29 | 2013-02-07 | 경상대학교산학협력단 | 미생물로부터 레티노이드를 생산하는 방법 |
| SG11201403163QA (en) | 2011-12-23 | 2014-09-26 | Danisco Us Inc | Enhanced production of isoprene using host cells having decreased ispa activity |
| JP2016511630A (ja) * | 2012-12-21 | 2016-04-21 | ダニスコ・ユーエス・インク | 代案の下流メバロン酸経路を使用するイソプレン、イソプレノイド、およびイソプレノイド前駆体の生成 |
| KR20160045001A (ko) | 2014-10-16 | 2016-04-26 | 명지대학교 산학협력단 | D-갈락토오스로부터 아이소프렌을 생산하는 능력을 가지는 재조합 대장균, 이의 제조방법 및 이를 이용하여 아이소프렌을 생산하는 방법 |
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| Journal Of Bacteriology, Nov. 2008, Vol 190, No. 22, pp.7491-7499 |
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| US11549117B2 (en) | 2023-01-10 |
| KR102106038B1 (ko) | 2020-04-29 |
| EP3623480A1 (en) | 2020-03-18 |
| KR102409420B9 (ko) | 2023-02-23 |
| KR20180124777A (ko) | 2018-11-21 |
| KR20210100585A (ko) | 2021-08-17 |
| KR102409420B1 (ko) | 2022-06-15 |
| KR20200044781A (ko) | 2020-04-29 |
| EP3623480A4 (en) | 2021-02-24 |
| US20200199602A1 (en) | 2020-06-25 |
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