KR20180124777A - 형질전환 생물체 선별용 마커 조성물, 형질전환 생물체 및 형질전환 방법 - Google Patents

형질전환 생물체 선별용 마커 조성물, 형질전환 생물체 및 형질전환 방법 Download PDF

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KR20180124777A
KR20180124777A KR1020180054080A KR20180054080A KR20180124777A KR 20180124777 A KR20180124777 A KR 20180124777A KR 1020180054080 A KR1020180054080 A KR 1020180054080A KR 20180054080 A KR20180054080 A KR 20180054080A KR 20180124777 A KR20180124777 A KR 20180124777A
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gene
pathway
enzyme
plasmid
synthase
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김선원
한효정
김신영
박지빈
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경상대학교산학협력단
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Priority to PCT/KR2018/005425 priority patent/WO2018208116A1/ko
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Abstract

본 발명은 항생제 및 항생제 저항성 유전자 없이 형질 전환 및 목적 산물의 생산이 가능하도록 하는 형질전환 생물체 선별용 마커 조성물, 형질전환 생물체, 형질전환 방법 및 목적 산물의 생산 방법에 관한 것으로, 항생제 및 항생제 저항성 유전자 사용에 기인한 문제를 원천적으로 예방하고, 목적 산물을 높은 수율로 생산할 수 있는 형질전환 생물체 선별용 마커 조성물, 형질전환 생물체, 형질전환 방법 및 목적 산물의 생산 방법에 관한 것이다.

Description

형질전환 생물체 선별용 마커 조성물, 형질전환 생물체 및 형질전환 방법{MARKER COMPOSITION FOR TRANSFORMED ORGANISM, TRANSFORMED ORGANISM AND METHOD FOR TRANSFORMATION}
본 발명은 형질전환 생물체 선별용 마커 조성물, 형질전환 생물체 및 형질전환 방법에 관한 것이다.
유용 물질들의 산업적인 생산은 최근에는 대부분 LMO를 이용하고 있다. 이 경우에 대사경로 및 목적산물 생합성 경로의 발현 강화를 위해서 관련 유전자들을 다양한 발현 벡터(플라스미드)들 이용해서 발현을 유도하게 된다. 이 경우에 벡터들은 항생제 저항성 유전자를 선택 마커로 갖고 있으며 배양 과정 동안 숙주 세포 안에서 플라스미드의 안정한 유지를 위해서 배양액에 항생제를 첨가하게 된다. 그러나 배양 공정에서 항생제를 사용하게 되면 고가의 항생제 사용으로 인한 생산비용 상승, 항생제 유출로 인한 환경오염 및 자연계에 항생제 저항성 돌연연이 생물체 발생위험, 최종 산물에 항생제가 잔류하여 추가 분리 정제 공정의 필요, 항생제 저항성 유전자 마커 함유 균주 사용 및 허가 취득의 어려움 등의 문제가 있다.
아울러 항생제 사용 배양의 경우에 배양시간이 길어지면 항생제의 분해 및 변형으로 인해서 항생제 저항성 유전자를 선택마커로 갖고 있는 플라스미드의 소실이 발생하고 배양 후반기의 생산성의 급격한 감소를 초래한다. 항생제의 분해 및 변형은 항생제 마커 유전자에서 발현된 효소에 의한 것과 항생제의 불안정성에 의한 자연발생적인 것으로 2차 산물 생산 등의 장시간 발효를 요하는 배양공정에서 심각한 문제를 유발한다.
이런 문제를 해결하기 위해서 목적산물 생산에 필요한 외래 유전자들을 숙주 생물체의 염색체에 삽입하는 방법도 있지만, 복수의 유전자 카피를 갖는 플라스미드 도입에 비해 유전자량의 감소에 따른 단백질 발현량 감소, 염색체에의 다수의 유전자 도입 및 발현의 어려움 등의 문제가 있다.
상기의 이유들로 항생제 마커프리(antibiotic marker-free) 생물체를 개발하는 것이 최근 생물공정 산업계에서는 이슈가 되고 있다. 그러나 현재까지는 산업계에서 안정적으로 유용하게 사용할 수 있는 항생제 마커프리 시스템은 없거나 극히 제한적이다. 영양 요구성 변이주에서 영양요구성 마커(auxotrophic selection marker)를 항생제 마커 대신에 사용할 수도 있지만 이 경우에는 일반적으로 많이 사용되는 산업용 배지인 복합배지를 사용할 수 없다는 단점이 있다.
또 다른 예로 Delphi Genetics사가 개발한 StabyExpress™이 있다(http://www.genengnews.com/gen-articles/protein-bioproduction-without-antibiotics/3891). 이것은 세균에 존재하는 antidote/poison 시스템인 ccd operon (ccdA and ccdB)을 이용하는 것이다. 그러나 이것은 일부 세균에서만 작동을 하고, ccdA / ccdB의 발현 비율이 정확하게 안 맞으면 작동을 안 하는 경우가 빈번하다.
한국등록특허 제1190255호
본 발명은 항생제 및 항생제 저항성 마커를 대체할 수 있는 형질전환 생물체 선별용 마커 조성물을 제공하는 것을 목적으로 한다.
본 발명은 항생제 및 항생제 저항성 마커 사용을 요하지 않는 형질전환 방법 및 형질전환 생물체를 제공하는 것을 목적으로 한다.
1. 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로의 효소를 코딩하는 유전자 중 적어도 하나가 도입된 플라스미드를 포함하는 형질전환 생물체 선별용 마커 조성물.
2. 위 1에 있어서, 상기 생물체는 내재적으로 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로를 갖는 것인 형질전환 생물체 선별용 마커 조성물.
3. 위 1에 있어서, 상기 합성 경로는 MEP 경로 또는 MVA 경로인 형질전환 생물체 선별용 마커 조성물.
4. 위 1에 있어서, 상기 합성 경로의 효소를 코딩하는 유전자는 1-디옥시-D-자이룰로스-5-포스페이트(DXP) 신타아제, DXP 리덕토이소머라아제, 2-C-메틸-D-에리스리톨-4-포스페이트(MEP) 시티딜트랜스퍼라아제, 4-디포스포시티딜-2-C-메틸-D-에리스리톨 키나아제, 2-C-메틸-D-에리스리톨-2,4-시클로디포스페이트(MEcPP) 신타아제, 4-히드록시-3-메틸-2-부테닐 디포스페이트 (HMBPP) 신타아제, HMBPP 리덕타아제, 아세토아세틸-CoA 신타아제, 3-히드록실-3-메틸글루타리-CoA (HMG-CoA) 신타아제, HMG-CoA 리덕타아제, 메발로네이트 키나아제, 메발로네이트-5-포스페이트 키나아제, 메발로네이트-5-디포스페이트 디카르복실라아제 및 IPP 아이소머라아제로 이루어진 군에서 선택된 1종 이상의 효소를 코딩하는 유전자인 형질전환 생물체 선별용 마커 조성물.
5. 위 1에 있어서, 상기 조성물은 상기와 동일 효소를 코딩하는 유전자 또는 이의 보완 유전자가 감쇄 또는 결손된 생물체에 형질전환 되는 것인 형질전환 생물체 선별용 마커 조성물.
6. 위 5에 있어서, 상기 유전자는 MEP 경로의 효소를 코딩하는 유전자이고, 상기 보완 유전자는 MVA 경로의 효소 중 적어도 하나를 코딩하는 유전자인 형질전환 생물체 선별용 마커 조성물.
7. 위 6에 있어서, 상기 보완 유전자는 아세토아세틸-CoA 신타아제, 3-히드록실-3-메틸글루타리-CoA (HMG-CoA) 신타아제, HMG-CoA 리덕타아제, 메발로네이트 키나아제, 메발로네이트-5-포스페이트 키나아제, 메발로네이트-5-디포스페이트 디카르복실라아제 및 IPP 아이소머라아제를 코딩하는 유전자인 형질전환 생물체 선별용 마커 조성물.
8. 위 5에 있어서, 상기 유전자는 MVA 경로의 효소를 코딩하는 유전자이고, 상기 보완 유전자는 MEP 경로의 효소 중 적어도 하나를 코딩하는 유전자인 형질전환 생물체 선별용 마커 조성물.
9. 위 1에 있어서, 상기 유전자 중 적어도 2개를 포함하고, 이들은 별개의 플라스미드에 각각 도입된 것인 형질전환 생물체 선별용 마커 조성물.
10. 위 1에 있어서, 상기 플라스미드는 이소프레노이드, 산탈렌, 비사볼롤 및 레티놀 합성 경로 이루어진 군에서 선택된 경로의 효소를 코딩하는 유전자가 더 도입된 것인 형질전환 생물체 선별용 마커 조성물.
11. 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로의 효소를 코딩하는 유전자 중 적어도 하나가 감쇄 또는 결손되고, 상기 감쇄 또는 결손된 유전자와 동일 효소를 코딩하는 유전자 또는 그 보완 유전자가 도입된 플라스미드로 형질전환된 생물체.
12. 위 11에 있어서, 상기 생물체는 내재적으로 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로를 갖는 것인 생물체.
13. 위 11에 있어서, 상기 합성 경로는 MEP 경로 또는 MVA 경로인 생물체.
14. 위 11에 있어서, 상기 합성 경로의 효소를 코딩하는 유전자는 1-디옥시-D-자이룰로스-5-포스페이트(DXP) 신타아제, DXP 리덕토이소머라아제, 2-C-메틸-D-에리스리톨-4-포스페이트(MEP) 시티딜트랜스퍼라아제, 4-디포스포시티딜-2-C-메틸-D-에리스리톨 키나아제, 2-C-메틸-D-에리스리톨-2,4-시클로디포스페이트(MEcPP) 신타아제, 4-히드록시-3-메틸-2-부테닐 디포스페이트 (HMBPP) 신타아제, HMBPP 리덕타아제, 아세토아세틸-CoA 신타아제, 3-히드록실-3-메틸글루타리-CoA (HMG-CoA) 신타아제, HMG-CoA 리덕타아제, 메발로네이트 키나아제, 메발로네이트-5-포스페이트 키나아제, 메발로네이트-5-디포스페이트 디카르복실라아제 및 IPP 아이소머라아제로 이루어진 군에서 선택된 1종 이상의 효소를 코딩하는 유전자인 생물체.
15. 위 11에 있어서, 상기 감쇄 또는 결손되는 유전자는 MEP 경로 상의 효소를 코딩하는 유전자인 생물체.
16. 위 15에 있어서, 상기 유전자는 DXP 신타아제 및 DXP 리덕토이소머라아제 중 적어도 하나를 코딩하는 유전자인 생물체.
17. 위 11에 있어서, 상기 감쇄 또는 결손되는 유전자는 MEP 경로 상의 효소를 코딩하는 유전자이고, 상기 보완 유전자는 MVA 경로 상의 효소 중 적어도 하나를 코딩하는 유전자인 생물체.
18. 위 17에 있어서, 상기 보완 유전자는 아세토아세틸-CoA 신타아제, 3-히드록실-3-메틸글루타리-CoA (HMG-CoA) 신타아제, HMG-CoA 리덕타아제, 메발로네이트 키나아제, 메발로네이트-5-포스페이트 키나아제, 메발로네이트-5-디포스페이트 디카르복실라아제 및 IPP 아이소머라아제를 코딩하는 유전자인 생물체.
19. 위 11에 있어서, 상기 감쇄 또는 결손되는 유전자는 MVA 경로 상의 효소를 코딩하는 유전자인 생물체.
20. 위 11에 있어서, 상기 플라스미드는 이소프레노이드, 산탈렌, 비사볼롤 및 레티놀 합성 경로 이루어진 군에서 선택된 경로의 효소를 코딩하는 유전자가 더 도입된 것인 생물체.
21. 형질전환 대상 균주의 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로의 효소를 코딩하는 유전자 중 적어도 하나를 감쇄 또는 결손시키는 단계; 및
상기 균주를 감쇄 또는 결손된 유전자와 동일 효소를 코딩하는 유전자 또는 이의 보완 유전자가 도입된 재조합 플라스미드로 형질전환시키는 단계;를 포함하는 생물체의 형질 전환 방법.
22. 위 21에 있어서, 상기 생물체는 내재적으로 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로를 갖는 것인 생물체의 형질 전환 방법.
23. 위 21에 있어서, 상기 형질 전환은 항생제 없이 수행되는 것인 생물체의 형질 전환 방법.
24. 위 21에 있어서, 상기 합성 경로는 MEP 경로 또는 MVA 경로인 생물체의 형질 전환 방법.
25. 위 21에 있어서, 상기 합성 경로의 효소를 코딩하는 유전자는 1-디옥시-D-자이룰로스-5-포스페이트(DXP) 신타아제, DXP 리덕토이소머라아제, 2-C-메틸-D-에리스리톨-4-포스페이트(MEP) 시티딜트랜스퍼라아제, 4-디포스포시티딜-2-C-메틸-D-에리스리톨 키나아제(IspE), 2-C-메틸-D-에리스리톨-2,4-시클로디포스페이트(MEcPP) 신타아제, 4-히드록시-3-메틸-2-부테닐 디포스페이트 (HMBPP) 신타아제, HMBPP 리덕타아제, 아세토아세틸-CoA 신타아제, 3-히드록실-3-메틸글루타리-CoA (HMG-CoA) 신타아제, HMG-CoA 리덕타아제, 메발로네이트 키나아제, 메발로네이트-5-포스페이트 키나아제, 메발로네이트-5-디포스페이트 디카르복실라아제 및 IPP 아이소머라아제 로 이루어진 군에서 선택된 1종 이상의 효소를 코딩하는 유전자인 생물체의 형질 전환 방법.
26. 위 21에 있어서, 상기 감쇄 또는 결손되는 유전자는 MEP 경로 상의 효소를 코딩하는 유전자인 생물체의 형질 전환 방법.
27. 위 26에 있어서, 상기 유전자는 DXP 신타아제 및 DXP 리덕토이소머라아제 중 적어도 하나를 코딩하는 유전자인 생물체의 형질 전환 방법.
28. 위 21에 있어서, 상기 감쇄 또는 결손되는 유전자는 MEP 경로 상의 효소를 코딩하는 유전자이고, 상기 보완 유전자는 MVA 경로 상의 효소 중 적어도 하나를 코딩하는 유전자인 생물체의 형질 전환 방법.
29. 위 28에 있어서, 상기 보완 유전자는 아세토아세틸-CoA 신타아제, 3-히드록실-3-메틸글루타리-CoA (HMG-CoA) 신타아제, HMG-CoA 리덕타아제, 메발로네이트 키나아제, 메발로네이트-5-포스페이트 키나아제, 메발로네이트-5-디포스페이트 디카르복실라아제 및 IPP 아이소머라아제를 코딩하는 유전자인 생물체의 형질 전환 방법.
30. 위 21에 있어서, 상기 감쇄 또는 결손되는 유전자는 MVA 경로 상의 효소 중 적어도 하나를 코딩하는 유전자인 생물체의 형질 전환 방법.
31. 위 21에 있어서, 상기 플라스미드는 이소프레노이드, 산탈렌, 비사볼롤 및 레티놀 합성 경로 이루어진 군에서 선택된 경로의 효소를 코딩하는 유전자를 더 포함하는 생물체의 형질 전환 방법.
32. 위 21에 있어서, 적어도 2개의 유전자를 감쇄 또는 결손시키고, 상기 균주를 이와 동일한 효소를 코딩하는 유전자를 각각 포함하는 적어도 2개의 플라스미드로 형질전환시키는 생물체의 형질 전환 방법.
33. 위 11 내지 20 중 어느 한 항의 생물체를 기질이 포함된 배지 상에서 배양하는 단계를 포함하는 목적 산물의 생산 방법.
34. 위 33에 있어서, 상기 배지는 항생제를 포함하지 않는 것인 목적 산물의 생산 방법.
본 발명의 형질전환 생물체 선별용 마커 조성물은 항생제 저항성 유전자를 사용하지 않는다. 이에, 항생제 및 항생제 저항성 유전자의 사용 없이도 형질 전환이 가능하여, 항생제 및 항생제 저항성 유전자 사용에 기인하는 많은 문제들을 원천적으로 방지할 수 있다.
본 발명의 형질전환 생물체 선별용 마커 조성물은 생물체의 장시간 배양에도 소실이 적다.
본 발명의 생물체는 항생제, 항생제 저항성 유전자 없이 형질전환 및 목적 산물의 생산이 가능하여, 항생제 및 항생제 저항성 유전자 사용에 기인하는 많은 문제들을 원천적으로 방지할 수 있다.
본 발명의 형질전환 방법은 항생제, 항생제 저항성 유전자 없이 형질전환이 가능하다.
본 발명의 목적 산물의 생산 방법은 높은 수율로 목적 산물을 생산할 수 있다.
도 1은 IPP와 DMAPP 생합성 경로이다.
도 2는 레티노이드의 HPLC 분석 프로파일(Profile)이다.
도 3은 레티노이드 정량분석을 위한 표준곡선이다.
도 4는 산탄렌의 GC 분석 프로파일이다.
도 5는 비사볼롤의 GC 분석 프로파일이다.
도 6은 비사볼롤 정량분석을 위한 표준곡선
도 7은 발효 부산물 생성 경로이다.
도 8은 MEP 경로 결손주에서 외래 MVA 경로를 보완하여 구축한 항생제 불필요 산탈렌 생산 균주의 항생제 유무에 따른 배양 결과이다.
도 9은 MEP 경로 결손주에서 외래 MVA 경로를 두 플라스미드로 나누어 구축한 항생제가 불필요한 레티노이드 생산 균주의 항생제 유무에 따른 배양 결과이다.
도 10은 항생제 마커가 제거된 레티노이드 생산 균주의 항생제 유무에 따른 배양 결과이다.
도 11은 결손된 MEP 경로 유전자를 선별마커로 이용한 산탈렌 생산 균주의 항생제 유무에 따른 배양 결과이다.
도 12는 결손된 MEP 경로 유전자를 복수 플라스미드에 나누어 구축하여 항생제가 불필요한 이소프레노이드(산탈렌, 비사볼롤) 생산 균주의 배양 결과이다.
도 13은 결손된 MEP 경로 유전자와 함께 외래 MVA 경로가 추가 도입된 항생제 마커가 제거된 레티노이드 생산 균주의 배양 결과이다.
도 14는 결손된 MEP 경로 유전자를 선별마커로 하여 항생제가 불필요한 균주의 형광 단백질 발현 결과이다.
도 15는 pCIN-mvaA-EGFP 재조합 플라스미드 모식도이다.
도 16은 결손된 MVA 경로 유전자를 선별마커로하여 항생제가 불필요한 균주의 형광 단백질 발현 결과이다.
이하 본 발명을 상세히 설명한다.
본 발명은 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로의 효소를 코딩하는 유전자 중 적어도 하나가 도입된 플라스미드를 포함하는 형질전환 생물체 선별용 마커 조성물을 제공한다.
이소펜테닐 디포스페이트(IPP) 또는 디메틸알릴 디포스페이트(DMAPP) 합성 경로는 모든 생명체가 필수적으로 갖고 있는 생합성 경로로서, IPP, DMAPP는 세포 내 필수 대사물질로 결핍이 되면 세포는 생장을 할 수 없다. 또한, 이들 물질은 강력한 음의 전하를 갖는 이인산화 물질로 배지 내에 존재해도 세포 내로 유입될 수가 없어서 반드시 세포 내에서 만들어져야 한다.
이에, 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로의 효소를 코딩하는 유전자 중 적어도 하나가 도입된 플라스미드를 포함하는 경우에 이를 형질전환 생물체 선별용 마커 조성물로 사용될 수 있다.
본 발명에 따른 생물체는 상기 이소펜테닐 디포스페이트(IPP) 또는 디메틸알릴 디포스페이트(DMAPP) 합성 경로를 내재적으로 가지는 것이라면 그 종류는 제한되지 않으며, 동물, 식물, 미생물을 포함하는 것이며, 구체적으로는 미생물일 수 있다.
이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로는 구체적으로 도 1에 도시된 MEP 경로 또는 MVA 경로일 수 있다.
이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로의 효소를 코딩하는 유전자는 예를 들면 MEP 경로의 1-디옥시-D-자이룰로스-5-포스페이트(DXP) 신타아제(1-deoxy-D-xylulose-5-phosphate synthase), DXP 리덕토이소머라아제(DXP reductoisomerase), 2-C-메틸-D-에리스리톨-4-포스페이트(MEP) 시티딜트랜스퍼라아제(2-C-methyl-D-erythritol-4-phosphate (MEP) cytidylyltransferase), 4-디포스포시티딜-2-C-메틸-D-에리스리톨 키나아제(4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, IspE), 2-C-메틸-D-에리스리톨-2,4-시클로디포스페이트(MEcPP) 신타아제(2-C-methyl-D-erythritol-2,4-cyclodiphosphate synthase), 4-히드록시-3-메틸-2-부테닐 디포스페이트 (HMBPP) 신타아제(4-hydroxy-3-methyl-2-butenyl diphosphate synthase), HMBPP 리덕타아제(HMBPP reductase), MVA 경로의 아세토아세틸-CoA 신타아제(acetoacetyl-CoA synthase), 3-히드록실-3-메틸글루타리-CoA (HMG-CoA) 신타아제(3-hydroxyl-3-methylglutary-CoA synthase), HMG-CoA 리덕타아제(HMG-CoA reductase), 메발로네이트 키나아제(mevalonate kinase), 메발로네이트-5-포스페이트 키나아제(mevalonate-5-phosphate kinase), 메발로네이트-5-디포스페이트 디카르복실라아제(mevalonate-5-diphosphate decarboxylase) 및 IPP 아이소머라아제(IPP isomerase) 등의 유전자를 들 수 있다.
본 발명의 형질전환 생물체 선별용 마커 조성물은 상기와 동일 효소를 코딩하는 유전자 또는 이의 보완 유전자가 감쇄 또는 결손된 생물체에 형질전환 되는 것일 수 있다.
전술한 바와 같이, 이소펜테닐 디포스페이트 및 디메틸알릴 디포스페이트 합성 경로가 불활성화되면 세포가 생존할 수 없게 되는데, 본 발명의 마커 조성물은 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로의 효소를 코딩하는 유전자 중 적어도 하나가 도입된 플라스미드를 포함하여, 이로 형질전환된 생물체는 결손된 IPP/DMAPP 경로가 다시 활성화되어 생존 가능하므로, 생물체의 생존 여부로 형질 전환 생물체를 확인할 수 있다.
형질전환 대상 생물체는 마커 조성물의 유전자와 동일 효소를 코딩하는 유전자 또는 이의 보완 유전자가 감쇄 또는 결손된 것일 수 있다.
동일 효소를 코딩하는 유전자인 경우 감쇄 또는 결손된 유전자와 동일한 유전자일 수도 있고, 다른 종 유래의 동일 효소를 코딩하는 유전자일 수도 있다.
구체적인 예를 들자면, 형질전환 대상 생물체는 DXP 리덕토이소머라아제를 코딩하는 유전자가 감쇄 또는 결손된 것일 수 있고, 본 발명에 따른 플라스미드는 그와 동일한 유전자 또는 다른 종 유래(유전자는 상이)의 동일한 효소를 코딩하는 유전자가 도입된 것일 수 있다.
보완 유전자는 유전자의 감쇄 또는 결손에 의해 불활성화된 경로 이외의 경로를 활성화시켜 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트를 생산할 수 있도록 하는 유전자를 의미한다. 예를 들면 생물체가 MEP 경로의 유전자가 감쇄 또는 결손된 경우에 마커 조성물의 플라스미드는 MVA 경로 전체의 효소를 코딩하는 유전자가 도입된 것일 수 있고, 생물체가 MVA 경로의 유전자가 감쇄 또는 결손된 경우에 마커 조성물의 플라스미드는 MEP 경로 전체의 효소를 코딩하는 유전자가 도입된 것일 수 있다.
본 발명의 조성물이 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로의 효소를 코딩하는 유전자 중 적어도 2개 이상이 도입된 플라스미드를 포함하는 경우에, 하나의 플라스미드에 2개 이상의 유전자가 도입된 것일 수도 있고, 별개의 플라스미드에 각각 유전자가 도입된 것일 수도 있다.
별개의 플라스미드에 각각 유전자가 도입된 경우에는 생물체가 모든 플라스미드로 형질전환되어야 생존할 수 있으므로, 생존하는 생물체를 모든 플라스미드로 형질전환된 생물체로서 선별할 수 있다.
구체적인 예를 들자면, 형질전환 대상 생물체는 DXP 신타아제를 코딩하는 유전자 및 DXP 리덕토이소머라아제를 코딩하는 유전자 중 적어도 하나가 감쇄 또는 결손된 것일 수 있고, 본 발명의 조성물은 그와 동일한 유전자 또는 다른 종 유래(유전자는 상이)의 동일한 효소를 코딩하는 유전자가 각각 도입된 2개의 플라스미드를 포함할 수 있다.
통상 생물체의 형질전환은 항생제가 존재하는 배양액 상에서 수행되어, 항생제 저항성 유전자가 선택 마커로서 사용되나, 그러한 경우에 고가의 항생제 사용에 의한 생산 비용 상승, 항생제 유출에 따른 환경 오염, 항생제 저항성 돌연변이 생물체 발생 위험 등의 문제가 있다.
또한, 항생제 저항성 유전자가 숙주 세포를 완벽하게 보호하는 것은 아니므로, 항생제에 의한 숙주 세포의 손상이 발생할 수 있고, 이를 최소화하는 항생제 사용 최적 농도를 설정하고 이를 유지하는 것은 산업계에서 매우 어려운 일로 인식되며, 이는 숙주 세포의 변경마다 달라질 수 있다.
더욱이, 항생제 사용 배양의 경우에 배양 시간이 길어지면 항생제의 분해 및 변형으로 인해서 항생제 저항성 유전자를 선택마커로 갖고 있는 플라스미드의 소실이 발생하고 배양 후반기의 생산성의 급격한 감소를 초래할 수 있다. 항생제의 분해 및 변형은 항생제 마커 유전자에서 발현된 효소에 의한 것과 항생제의 불안정성에 의한 자연발생적인 것으로 2차 산물 생산 등의 장시간 발효를 요하는 배양공정에서 심각한 문제를 유발한다.
그러나, 본 발명의 상기 형질전환 생물체 선별용 마커 조성물을 사용함으로써 상기 문제점들의 발생을 원천적으로 방지하고, 항생제를 사용하지 않고도 플라스미드를 숙주 생물체 내에서 안전하게 유지하고, 형질전환 생물체를 선별할 수 있다.
또한, 그러한 플라스미드에 목적 산물 생산을 위한 경로의 효소를 코딩하는 유전자를 추가로 도입하여, 목적 산물을 숙주 생물체에서 안정적으로 대량 생산하는 데에 활용할 수도 있다.
또한, 본 발명은 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로의 효소를 코딩하는 유전자 중 적어도 하나가 감쇄 또는 결손되고, 상기 감쇄 또는 결손된 유전자와 동일한 효소를 코딩하는 유전자 또는 그 보완 유전자가 도입된 플라스미드로 형질전환된 생물체를 제공한다.
이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로는 MEP 경로 또는 MVA 경로일 수 있고, 이들 효소는 전술한 바와 같다.
본 발명의 생물체에서 감쇄 또는 결손되는 유전자는 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로의 효소를 코딩하는 유전자 중 적어도 하나, 구체적으로 MEP 경로 또는 MVA 경로 상의 효소를 코딩하는 유전자 중 적어도 하나일 수 있다.
본 발명의 생물체는 감쇄 또는 결손된 유전자와 동일한 유전자 또는 다른 종 유래의 동일한 효소를 코딩하는 유전자가 도입된 플라스미드로 형질전환된 것일 수 있다.
구체적인 예를 들자면, DXP 신타아제 및 DXP 리덕토이소머라아제를 코딩하는 유전자 중 적어도 하나가 감쇄 또는 결손된 것일 수 있고, 그와 동일한 유전자 또는 다른 종 유래(유전자는 상이)의 동일한 효소를 코딩하는 유전자가 도입된 플라스미드로 형질전환된 것일 수 있다.
또한, 본 발명의 생물체는 감쇄 또는 결손된 유전자의 보완 유전자가 도입된 플라스미드로 형질전환된 것일 수 있다.
보완 유전자는 유전자의 감쇄 또는 결손에 의해 불활성화된 경로 이외의 경로를 활성화시켜 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트를 생산할 수 있도록 하는 유전자를 의미한다. 예를 들면 생물체가 MEP 경로의 유전자가 감쇄 또는 결손된 경우에 MVP 경로 전체의 효소를 코딩하는 유전자가 도입된 플라스미드로 형질전환된 것일 수 있고, 생물체가 MVA 경로의 유전자가 감쇄 또는 결손된 경우에 MEP 경로 전체의 효소를 코딩하는 유전자가 도입된 플라스미드로 형질전환된 것일 수 있다.
본 발명의 생물체는 목적 산물 생산을 위한 목적 산물 합성 경로의 효소를 코딩하는 유전자로 더 형질전환된 것일 수 있다.
그 유전자는 목적하는 산물에 따라 다양하게 선택될 수 있으며, 예를 들면 이소프레노이드 합성 경로, 산탈렌 합성 경로, 레티놀 합성 경로, 비사볼롤 합성 경로 등을 들 수 있으나, 이에 제한되는 것은 아니다. 상기 각 합성 경로는 모두 공지된 경로로서, 공지된 경로의 효소를 코딩하는 유전자로 형질전환될 수 있다.
또한, 본 발명의 생물체는 상기 목적 산물 합성 경로에서 목적 산물의 부산물 생성 경로의 효소를 코딩하는 유전자가 감쇄 또는 결실된 것일 수 있다. 이에 의해 목적 산물 생산 수율이 더욱 향상될 수 있다. 예를 들면 MVA 경로의 출발 물질인 acetyl-CoA를 발효부산물인 acetate, lactate, ethanol로 전환시키는 효소들을 들 수 있고, 구체적으로 아세트알데히드 디하이드로게나아제(adhE), 파루베이트 옥시다아제(PoxB), 락테이트 디하이드로게나아제(ldhA), 아세틸-COA:아세토아세틸-CoA 신타아제(atoDA) 등을 들 수 있으나, 이에 제한되는 것은 아니다(도 7 참조).
본 발명의 생물체는 항생제 저항성 유전자를 포함하지 않을 수 있다. 항생제 저항성 유전자를 마커로 사용하지 않으므로 이를 포함하는 것이 불필요하다. 물론, 전술한 형질전환 이후에 추후 항생제 저항성 유전자가 도입된 플라스미드로 추가로 형질전환되어 항생제 저항성 유전자를 포함할 수도 있다.
본 발명은 형질전환 대상 생물체의 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로의 효소를 코딩하는 유전자 중 적어도 하나를 감쇄 또는 결손시키는 단계; 및 상기 생물체를 감쇄 또는 결손된 유전자와 동일한 효소를 코딩하는 유전자 또는 그 보완 유전자가 도입된 재조합 플라스미드로 형질전환시키는 단계;를 포함하는 생물체의 형질 전환 방법을 제공한다.
이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로의 효소를 코딩하는 유전자는 전술한 범위 내의 유전자일 수 있다.
전술한 바와 같이 통상 생물체의 형질 전환은 항생제를 포함한 배양액 상에서 수행되는 것이지만, 본 발명의 형질 전환 방법은 항생세 저항성 마커를 사용하지 않으므로, 본 발명의 형질 전환은 항생제 없이 수행될 수 있다.
상기 감쇄 또는 결손되는 유전자는 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로의 효소를 코딩하는 유전자 중 적어도 하나일 수 있다. 구체적으로, MEP 경로 또는 MVA 경로 상의 효소를 코딩하는 유전자일 수 있다.
형질전환시에는 감쇄 또는 결손된 유전자와 동일한 유전자 또는 다른 종 유래의 동일한 효소를 코딩하는 유전자가 도입된 플라스미드로 형질전환시킬 수 있다.
구체적인 예를 들자면, DXP 신타아제 및 DXP 리덕토이소머라아제 중 적어도 하나를 코딩하는 유전자가 감쇄 또는 결손될 수 있고, 그와 동일한 유전자 또는 다른 종 유래(유전자는 상이)의 동일한 효소를 코딩하는 유전자가 도입된 플라스미드로 형질전환될 수 있다.
또한, 감쇄 또는 결손된 유전자의 보완 유전자가 도입된 플라스미드로 형질전환될 수 있다.
보완 유전자는 유전자의 감쇄 또는 결손에 의해 불활성화된 경로 이외의 경로를 활성화시켜 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트를 생산할 수 있도록 하는 유전자를 의미한다. 예를 들면 생물체가 MEP 경로의 유전자가 감쇄 또는 결손된 경우에 MVP 경로 전체의 효소를 코딩하는 유전자가 도입된 플라스미드로 형질전환 될 수 있고, 생물체가 MVA 경로의 유전자가 감쇄 또는 결손된 경우에 MEP 경로 전체의 효소를 코딩하는 유전자가 도입된 플라스미드로 형질전환 될 수 있다.
2개 이상의 유전자가 도입되는 경우에 이들은 단일 플라스미드에 도입될 수도 있고, 복수개의 플라스미드에 각각 도입될 수도 있다.
비제한적인 보다 구체적인 결손 및 형질전환 방법의 예시를 들자면, 전술한 바와 같이 IPP, DMAPP가 없으면 생물체가 생존할 수 없으므로, MEP 경로의 dxs 또는 ispC의 결손시에 배지에 2-C-메틸-D-에리스리톨을 첨가하여 ispC의 대사산물인 2-C-메틸-D-에리스리톨 4-포스페이트(MEP)를 생성하여 생물체의 생육을 유지시킨 상태에서 상기 생물체를 이와 동일한 효소를 코딩하는 유전자 또는 그 보완 유전자로 형질전환시킬 수 있다. 또한, 먼저 MVA 하부 경로 유전자를 도입하고, 메바론산이 첨가된 배지에서 MEP 경로 유전자의 감쇄 또는 결손을 수행할 수도 있다.
MVA 경로 유전자의 결손시에는 상부 경로, 하부 경로 감쇄 또는 결손에 따라 구체적인 방법이 달라질 수 있다.
상부 경로 결손의 구체적인 예를 들자면 배지에 메바론산을 첨가하여 하부 경로만으로 생육을 유지시킨 상태에서, 상부 경로 유전자를 감쇄 또는 결손시키고 이와 동일한 효소를 코딩하는 유전자 또는 그 보완 유전자로 형질전환시킬 수 있다.
하부 경로 결손의 구체적인 예를 들자면, MEP 경로 유전자 모두를 도입하여 생육을 유지시킨 상태에서, 상부 경로 유전자를 감쇄 또는 결손시키고 이와 동일한 효소를 코딩하는 유전자 또는 그 보완 유전자로 형질전환시킬 수 있다.
본 발명에 따른 플라스미드는 목적 산물 생산을 위한 목적 산물 생산 경로의 효소를 코딩하는 유전자를 더 포함할 수 있다.
그 유전자는 목적하는 산물에 따라 다양하게 선택될 수 있으며, 예를 들면 이소프레노이드 합성 경로, 산탈렌 합성 경로, 레티놀 합성 경로, 비사볼롤 합성 경로 등을 들 수 있으나, 이에 제한되는 것은 아니다. 상기 각 합성 경로는 모두 공지된 경로로서, 공지된 경로의 효소를 코딩하는 유전자가 더 도입될 수 있다.
또한, 본 발명에 따른 생물체는 상기 목적 산물 합성 경로에서 목적 산물의 부산물 생성 경로의 효소를 코딩하는 유전자가 감쇄 또는 결실된 것일 수 있다. 이에 의해 목적 산물 생산 수율이 더욱 향상될 수 있다. 예를 들면 MVA 경로의 출발 물질인 acetyl-CoA를 발효부산물인 acetate, lactate, ethanol로 전환시키는 효소들을 들 수 있고, 구체적으로 아세트알데히드 디하이드로게나아제(adhE), 파루베이트 옥시다아제(PoxB), 락테이트 디하이드로게나아제(ldhA), 아세틸-COA:아세토아세틸-CoA 신타아제(atoDA) 등을 들 수 있으나, 이에 제한되는 것은 아니다(도 7 참조).
또한, 본 발명은 상기 생물체를 이용한, 또는 상기 형질전환 방법을 포함하는 목적 산물의 생산 방법을 제공한다.
본 발명의 목적 산물의 생산 방법은 상기 생물체를 목적 산물 생산 경로의 효소를 코딩하는 유전자로 형질전환시켜 목적 산물을 생산한다.
목적 산물 생산 경로의 효소를 코딩하는 유전자는 전술한 플라스미드에 도입되어 생물체에 형질전환될 수 있다.
목적 산물은 상기 생물체를 기질을 포함하는 배지 중에서 배양하여 생산할 수 있고, 배양은 항생제를 포함하지 않는 배양 조건에서 수행될 수 있다.
본 발명의 방법은 항생제 및 항생제 저항성 유전자 없이도 형질전환 및 목적 산물의 생산이 가능하여, 항생제 사용에 의한 문제를 원천적으로 방지할 수 있고, 항생제의 분해 및 변형에 의한 플라스미드의 소실 문제가 발생하지 않으므로, 보다 높은 수율로 목적 산물을 생산할 수 있다.
이하, 본 발명을 구체적으로 설명하기 위해 실시예를 들어 상세하게 설명하기로 한다.
실시예
1. 재료 및 방법
1) 실험균주 및 재료
실험에 이용된 미생물은 ATCC (American Type Culture Collection), KCTC (Korea Collection for Type Cultures), 및 KCCM (Korea Culture Center of Microorganisms)에서 구입하였으며, 표 1에 정리하였다.
실험균주 설명 정보 또는 참고문헌
DH5a F-, λ-, endA1, glnV44, thi-1, recA1, relA1, gyrA96, deoR, φ80, dlacZ △15, △lacZYA - argF) U169, hsdR17(rK -
mK +), supE44		 NEB C2987
MG1655(DE3) F-　λ- ilvG rfb -50 rph -1　(DE3) KCCM 41310
MG1655(DE3) DadhE ::MVA bottom DadhE (::PcTrc -SN12Didi-ter) -
MG1655(DE3) DatoDA::MVA bottom DatoDA (::PcTrc -SN12Didi-ter) -
MG1655(DE3) DldhA::MVA bottom DldhA (::PcTrc -SN12Didi-ter) -
MG1655(DE3) DpoxB::MVA bottom DpoxB (::PcTrc -SN12Didi-ter) -
MG1655(DE3)
Δdxr
DadhE ::MVA bottom		 Δdxr DadhE (::PcTrc -SN12Didi-ter) -
MG1655(DE3)
Δdxr/s
DadhE ::MVA bottom		 Δdxr, Δdxs, DadhE (::PcTrc -SN12Didi-ter) -
E. coli EC1000 RepA+ MC1000, KmR , carrying a single copy of the pWV01 repA gene in the glgB gene; host for pOR128-based plasmids Leenhouts et al Mol Gen Genet. 1996 Nov 27;253(1-2):217-24
L. lactis subsp. cremoris MG1363 Plasmid-free derivative of L. lactis subsp. Cremoris NCDO712 Plasmid complement of Streptococcus lactis NCDO712 and other lactic streptococci after protoplast-induced curing. J. Bacteriol.154:1-9.
L. lactis subsp. cremoris MG1363 ΔmvaA ΔmvaA 　-
유전자 클로닝에는 DH5α(F-f80dlacZDM15D(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK -, mK +) phoA supE44λ- thi-1 gyrA96 relA1)를 이용하였고, 목적물질 생산에는 MG1655(DE3)(F- λ- ilvG rfb-50 rph-1(DE3))를 사용하였다. 발현벡터로는 pTrc99A, pSTV28을 사용하였다(표 2). 제한효소 및 기타 효소는 New England Biolabs (미국) 제품을 사용하였다. 또한 PCR을 수행하기 위해서는 Pfu -X DNA polymerase는 Solgent (한국), Phusion DNA polymerase는 Thermo Scientific (미국) 제품을 사용하였다. DNA size maker는 Invitrogen (미국) 제품을 사용하였다. IPTG는 Promega(미국), L(+)-arabinose, glucose, lactose는 Sigma (미국), acetone은 Merck (미국), glycerol은 Amresco (미국) 제품을 사용하였다. 그 외 시약들은 Sigma (미국)의 제품을 사용하였다.
미생물 배양을 위한 배지의 제조는 각 균주 분양기관의 권장배지조성 및 Difco manual (11th edition, Difco; BD Science, US)에 따랐다. 배지 제조에 사용된 시약은 BD Science (미국) 및 Sigma (미국)로부터 구입하였다. 배양 중의 균체량은 spectrophotometer (Shimadzu UV-1601, Japan)로 600 nm에서 OD (optical density)를 측정한 결과로 나타내었으며, pH는 pH meter B-212 (HORIBA, Japan)로 측정하였다.
유전자 클로닝에는 플라스미드 유지를 위한 항생제로 ampicillin, kanamycin을 사용하였으며, 각각 100 ㎍/mL, 50 ㎍/mL 농도로 사용하였다.
2) 레티노이드의 추출 및 분석
레티노이드는 다음의 방법으로 분석하였다. 배양액 50 ~ 100 ㎕를 취해서 14,000 rpm에서 40초 동안 원심분리하여 균체를 회수하였다. 회수된 균체에 아세톤 400 ㎕을 첨가하여 균체를 재현탁시킨 다음 어두운 곳에서 55℃에서 15분 동안 추출하고, 이에 다시 600 ㎕의 아세톤을 첨가하여 55℃에서 15분 동안 추출하였다. 추출물은 14,000 rpm에서 10분 동안 원심분리 한 후 상층액만을 취하여 HPLC 정량 분석을 실시하였다. 그리고 레티노이드 배양액에 heavy mineral oil 층을 첨가한 경우 heavy mineral oil 층만을 14,000 rpm 에서 10분 동안 원심분리한 후 레티노이드를 함유하고 있는 heavy mineral oil 층 5 ~ 50 ㎕를 1 mL 아세톤에 재현탁하여 추출하였으며, 상온에서 15분간 방치하며 5분 간격으로 vortexing하였다. 추출물은 14,000 rpm에서 10분 동안 원심분리 한 후 acetone 층만을 취하여 HPLC 정량 분석을 실시하였다.
레티노이드의 분석 시스템은 SHIMADZU LC-20A series with UV/Vis detector (Shimadzu, Kyoto, Japan)을 이용하였으며 분석칼럼은 Symmetry C18 (250x4.6,5 ㎛) with Symmetry guard C18 (15x4.6, 5 ㎛)을 사용하였다. 이동상은 메탄올: 아세토니트릴 혼합용액(95:5, v/v)으로 총 15분간 분석하였다. 유속은 1.5 mL/min으로 하였으며 검출 파장은 각각 레티날은 370 nm, 레티놀과 레티닐 아세테이트는 340 nm에서 측정하였고, 시료 주입량은 20 ㎕, 오븐 온도는 40℃로 분석하였다. 레티노이드 표준시료는 에탄올에 녹여 사용하였다. 표준시간의 피크 머무름 시간은 레티놀 약 3.2분, 레티날 약 3.4분, 레티닐 아세테이트는 약 4.0분이며(도 2), 분석결과는 분석된 피크의 면적을 표준시료의 피크 면적으로 계산한 검량선에 대입하고 희석배수를 환산하여 계산하였다(도 3).
3) 산탈렌의 추출 및 분석
배지에 데케인을 도포하는 2상 배양에서 생산된 산탈렌 분석을 위해서 데케인 층의 gas chromatography (GC) 와 gas chromatography-mass spectrometry (GC-MS) 분석을 실시하였다(도 4). GC 분석은 19091N-133 HP-Innowax column(30 m; internal diameter, 0.25 mm; film thickness, 250 nm)이 장착된 GC/FID (Agilent Technologies7890A)를 이용하였다. 컬럼 오븐 온도는 초기 80℃에서 1 min, 그리고 10℃/min의 속도로 250℃까지 올리고 1분 동안 유지하였다. 이동상 기체로 질소를 39 psi의 압력으로 흘려주고, detector temperature는 260℃로 유지하였다. GC-MS 분석은 GCMS-QP2010 Ultra (SHIMADU, Tokyo, Japan)를 사용하였고 이동상 기체로는 helium을 이용하였다.
4) 비사볼롤의 추출 및 분석
배양 종료 후 데칸층을 원심분리(14,000 rpm, 10 min)를 통해 회수하여 gas chromatography (GC) 와 gas chromatography-mass spectrometry (GC-MS)로 분석하였다(도 5). GC 분석은 19091N-133 HP-Innowax column (30 m; internal diameter, 0.25 mm; film thickness, 250 nm)이 장착된 GC/FID (Agilent Technologies 7890A)를 이용하였다. 컬럼 오븐 온도는 초기 50℃에서 20℃/min의 속도로 증가시켰으며, 90℃ 도달 이후 15℃/min의 속도로 증가시켰다. 온도가 150℃에 도달한 후 20℃/min 속도로 190℃까지 온도를 증가 시킨 후 10℃/min의 속도로 260℃에 도달하게 한 후 2분 동안 유지하게 하였다. 이동상 기체로 질소를 30 psi의 압력으로 공급하였고, 검출기 온도는 280℃로 유지하였다. GC-MS 분석은 GCMS-QP2010 Ultra (SHIMADU, Japan)를 사용하였고 이동상 기체로는 헬륨을 이용하였다. 측정된 비사볼롤의 피크 면적 값은 미리 작성한 검량선(도 6)에 따라 다음과 같이 계산하였다.
[수학식 1]
α-Bisabolol (mg/L) = 0.8116 × GC Peak area × 희석배수
2. MEP 경로 결손 균주 구축 과정
- MEP 경로 결손 균주 구축 시 MVA 하부경로 유전자를 염색체에 도입하고 구축 작업은 메바론산 첨가 배지에서 진행한 예시
- MEP 상부경로 유전자 결손
1) 대장균 염색체 내 외래 MVA 하부경로 삽입
대장균 염색체 내 외래경로 삽입을 위한 방법은 λ-Red recombinase를 이용한 PCR 기반의 상동성 재조합 방법과 P1 형질도입 방법으로 크게 두 가지가 있다. 이번 실험에서는 λ-Red recombinase를 이용한 PCR 기반의 상동성 재조합 방법을 이용하여 외래 MVA 하부경로를 대장균 MG1655(DE3) 균주에 삽입해주었다. MVA 경로에서 mevalonate 이후부터 DMAPP까지의 경로를 하부 경로로 하였다(도 1 참조). 현재 MVA 하부경로는 Streptococcus pneumoniae의 mvaK1, mvaK2, mvaD와 E. coli의 idi 유전자로 이루어져 있으며, 서열번호 1과 서열번호 2의 프라이머를 이용해 상기의 유전자를 증폭해 pTFCC(DPB) 벡터에 클로닝하여 pTFCC(DPB)-SN12Didi를 구축하였다. MVA 하부경로 삽입위치는 adhE, poxB, ldhA, atoDA 네 가지 유전자에 각각 삽입하였다. 이는 MVA 경로의 출발물질인 acetyl-CoA를 발효부산물인 acetate, lactate, ethanol로 전환시키는 유전자들로 이를 차단하여 발효생산성 및 탄소원 이용효율을 향상시킬 수 있다(도 7).
실험방법은 다음과 같다. 먼저 대장균 염색체 내에 삽입할 경로가 균체 내에서 발현할 수 있도록 promoter, multi-cloning site, terminator, FRT site, 항생제 마커를 포함한 벡터를 구축하였으며, 이는 표 2에 나타내었다.
플라스미드 설명 Genbank 허가번호 제품 서열
번호
pSTV28 p15A origin, lac promoter, lacZ, and cat M22744 Takara Korea (한국) 71
pTrc99A pMB1 origin, trc promoter, lacI q , and bla U13872.1 Pharmacia (US) 72
pTFKC(DPB) ColE1 origin, trc promoter without operator region, kanamycin cassette with FRT, and bla 　- 　 73
pTFCC(DPB) ColE1 origin, trc promoter without operator region, chloramphenicol cassette with FRT, and bla 　- 　 74
pCP20 cI857λts, FLP, cat, and bla - 　 75
pKD46 repA101ts, oriR101, exo , bet, gam, tL3, PBAD, araC, and bla AY048746 　 76
pT-ispA-STS pTrc99A 벡터; lacIq; Ampr; E. coli유래 FPP Synthase인 ispA; Clausena lansium유래 santalene synthase 인 STS를 포함하는 Ptrc 발현벡터 - 　 77
pTAS-NA pTrc99A 벡터; lacIq; Ampr; E. coli유래 FPP Synthase인 ispA 및 idi; Clausena lansium유래 santalene synthase 인 STS; E. faecalis 유래 mvaE 및 mvaS; S.pneumonia 유래 mvaK1, mvaK2 및 mvaD 포함하는 Ptrc 발현벡터 -　 In this study 78
pSNAK pSTV28 벡터; lacZ; Kmr; E. faecalis 유래 mvaE 및 mvaS; S.pneumonia 유래 mvaK1, mvaK2 및 mvaD; E. coli 유래 idi를포함하는 Plac 발현 벡터 - 　 79
pSNAK(-E) pSTV28 벡터; lacZ; Kmr; E. faecalis 유래 mvaS; S.pneumonia 유래 mvaK1, mvaK2 및 mvaD; E. coli 유래 idi를 포함하는 Plac 발현 벡터 -　 In this study 80
pSNA(-E)free pSTV28 벡터; lacZ; E. faecalis 유래 mvaS; S.pneumonia 유래 mvaK1, mvaK2 및 mvaD; E. coli 유래 idi를 포함하는 항생제 마커가 없은 벡터 - In this study 81
pTEFAmvaE pTrc99A 벡터; lacIq; Ampr; E . faecalis 유래 mvaE를 포함하는 Ptrc 발현벡터 - 　 82
pT-DHBSRYbbO pTrc99A 벡터; lacIq; Ampr; P.agglomerans 유래 crtE, crtB 및 crtI; P. ananatis 유래의 crtY; 코돈최적화 된 uncultured marine bacterium 66A03 유래 SR; E. coli의 dxs 및 YbbO - 　 83
pT-HBSRYbbO pTrc99A 벡터; lacIq; Ampr; P.agglomerans 유래 crtE, crtB 및 crtI; P. ananatis 유래의 crtY; 코돈최적화 된 uncultured marine bacterium 66A03 유래 SR; E. coli의 YbbO 　- In this study 84
pT-HBSREYbbO pTrc99A 벡터; lacIq; Ampr; P.agglomerans 유래 crtE, crtB 및 crtI; P. ananatis 유래의 crtY; 코돈최적화 된 uncultured marine bacterium 66A03 유래 SR; E. coli의 YbbO; E. faecalis 유래 mvaE 　- In this study 85
pT-HBSREYbbOfree pTrc99A 벡터; lacIq; P.agglomerans 유래 crtE, crtB 및 crtI; P. ananatis 유래의 crtY; 코돈최적화 된 uncultured marine bacterium 66A03 유래 SR; E. coli의 YbbO; E. faecalis 유래 mvaE를 포함하는 항생제 마커가 없은 벡터 　- In this study 86
pT-dxr pTrc99A 벡터; lacIq; Ampr; E.coli 유래의 dxr를 포함하는 Ptrc 발현벡터 　- 　 87
pTAS-dxr pTrc99A 벡터; lacIq; Ampr; E. coli유래 FPP Synthase인 ispA 및 dxr; Clausena lansium유래 santalene synthase 인 STS 포함하는 Ptrc 발현벡터 　- In this study 88
pT-dxr/s pTrc99A 벡터; lacIq; Ampr; E.coli 유래의 dxr 및 dxs를 포함하는 Ptrc 발현벡터 　- 　 89
pT-ispA-MrBBS pTrc99A 벡터; lacIq; Ampr; E. coli유래 FPP Synthase인 ispA; 코돈최적화 된 Matricaria recutita유래의 α-bisabolol synthase 인 MrBBS를 포함하는 Ptrc 발현벡터 　- In this study 90
pTAS-dxs pTrc99A 벡터; lacIq; Ampr; E. coli유래 FPP Synthase인 ispA 및 dxs; Clausena lansium유래 santalene synthase 인 STS 포함하는 Ptrc 발현벡터 　- In this study 91
pTAB-idi-dxr pTrc99A 벡터; lacIq; Ampr; E. coli유래 FPP Synthase인 ispA 및 dxr; 코돈최적화 된 Matricaria recutita유래의 α-bisabolol synthase 인 MrBBS를 포함하는 Ptrc 발현벡터 　- In this study 92
pSNAK(-E)-dxs pSTV28 벡터; lacZ; Kmr; E. faecalis 유래 mvaS; S.pneumonia 유래 mvaK1, mvaK2 및 mvaD; E. coli 유래 idi 및 dxs를 포함하는 Plac 발현 벡터 　- In this study 93
pSNA(-E)-dxsfree pSTV28 벡터; lacZ; E. faecalis 유래 mvaS; S.pneumonia 유래 mvaK1, mvaK2 및 mvaD; E. coli 유래 idi 및 dxs를 포함하는 항생제 마커가 없은 벡터 　- In this study 94
pT-HBSREYbbOdxrfree pTrc99A 벡터; lacIq; P.agglomerans 유래 crtE, crtB 및 crtI; P. ananatis 유래의 crtY; 코돈최적화 된 uncultured marine bacterium 66A03 유래 SR; E. coli의 YbbO 및 dxr; E. faecalis 유래 mvaE를 포함하는 항생제 마커가 없은 벡터 　- In this study 95
pEGFP pUC origin, lac promoter; EGFP, and AmpR - Clontech (US) 96
pEGFP-dxr pUC origin, lac promoter; EGFP, E. coli의 dxr , and AmpR - In this study 97
pORI19 Emr Ori+ RepA- lacZ' derivative of pORI28 A System To Generate Chromosomal Mutations in Lactococcus lactis Which Allows Fast Analysis of Targeted Genes - 98
pORI19-mvaA pORI19-mvaA - In this study 99
pCI372 E. coli/L. lactis shuttle vector, CamR Identification of the minimal replicon of lactococcus lactis subsp. lactis UC317 plasmid pCI305 - 100
pCIN pCI372-FP promoter, MCS, rrnp terminator 　- In this study 101
pCIN-mvaA pCIN-mvaA 　- In this study 102
pCIN-mvaA-EGFP pCIN-mvaA,EGFP 　- In this study 103
구축된 벡터는 모두 trc promoter를 사용하거나, trc promoter를 항시 발현할 수 있도록 변형하여 사용하였다. 따라서 구축된 pBFKC의 경우 trc promoter를 가지며, pTFKC(DPB)와 pTFCC(DPB)의 경우 항시 발현할 수 있도록 변형된 trc promoter를 가진다. 구축된 벡터의 multi-cloning site를 이용하여 원하는 경로를 삽입하였으며, 각각의 구축된 plasmid를 주형으로 대장균 삽입할 부분과 50 bp의 상동성을 가지는 프라이머를 이용하여 PCR을 수행하였다. 각 프라이머 정보는 표 4에 서열번호 3부터 서열번호 10으로 나타내었다. 얻어진 PCR 산물은 정제하여 pKD46을 포함한 대장균 MG1655(DE3) 수용성 세포(competent cell)에 1mm 간격의 큐벳을 통해 1.8 kV로 electro-transformation하고, 곧바로 SOC medium (2% Bacto Tryptone, 0.5% yeast extract, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl2, 10 mM MgSO4, 20 mM glucose) 1mL을 첨가하여, 37℃에서 1시간 동안 진탕 배양 한 후, 항생제를 첨가한 평판배지에 도말하여 37℃에서 배양 후 단일 콜로니를 얻었다. 여기서 얻은 단일 콜로니들을 소량의 증류수에 현탁하여 10분간 가열하고, 이를 원심 분리한 후 상층액을 주형으로 하여 서열번호 11부터 서열번호 18까지의 각 프라이머로 PCR을 수행하여 경로삽입 여부를 확인하였다. 항생제 마커 제거를 위해서는 외래경로 삽입이 확인된 균주에 pCP20 플라스미드를 형질전환하여 30℃에서 배양 후 단일 콜로니를 얻었으며, 여기서 얻은 단일 콜로니들을 주형으로 하여 상기 확인용 프라이머를 통해 PCR을 수행하고 항생제 마커가 제거된 것을 확인하였다. 확인된 콜로니는 43℃에 배양하여 온도민감성 플라스미드인 pCP20 플라스드를 제거하여 완성된 외래경로 삽입균주를 얻을 수 있었다.
2) MG1655(DE3) ΔadhE :: MVAbottom 균주에서 dxr , dxs 유전자 결실
유전자 결실 또한 Datsenko와 Wanner의 방법을 이용하여 대장균 염색체 내 dxr 유전자를 결손하였다.
dxr 유전자를 상동성 재조합에 의하여 선별 마커 유전자인 카나마이신 유전자로 대체하기 위하여, pTFKC(DPB) 플라스미드의 카나마이신 유전자에 결합하면서 dxr 유전자의 상류 말단과 하류 말단의 염기 서열을 갖는 PCR 프라이머를 제조하였다. 정방향 프라이머는 dxr 유전자의 상류 말단의 50bp 염기서열 다음에, 카나마이신 유전자 결합 염기 서열 15bp로 구성되어 있으며, 역방향 프라이머는 dxr 유전자의 하류 말단의 50bp 염기서열 다음에, 카나마이신 유전자 결합 염기 서열 20bp로 구성되어 있다. 사용한 프라이머는 하기 표 7에 표시하였다. 서열번호 19과 서열번호 20의 올리고뉴클레오티드를 프라이머로 하고, pTFKC(DPB)을 주형으로 PCR 반응을 수행하였다.
상기의 정제된 PCR 반응산물은 pKD46 (Datsenko KA and Wanner BL, 2000 Proc Natl Acad Sci U.S.A., 97(12):6640-6645)을 포함한 MG1655(DE3)ΔadhE::MVAbottom 수용성 세포 (competent cell)로 형질전환하였다. 3.3mM mevalonate와 카나마이신을 포함한 평판 배지에서 콜로니를 얻고, 이 콜로니는 PCR을 통하여 카나마이신 유전자가 상동성 재조합에 의해서 dxr 유전자를 대체했는지를 확인할 수 있다. 또한 mevalonate가 없는 평판 배지에 도말하여 콜로니가 자라지 못하는 것으로 dxr 유전자의 결손에 따른 MEP 경로가 차단된 것을 확인할 수 있다. 콜로니 PCR에 사용된 프라이머는 대장균 MG1655(DE3) 염색체 상에서 dxr 유전자 바로 인접한 영역에 결합하도록 제조하였다. 사용한 확인용 프라이머는 서열번호 21, 22, 23의 올리고뉴클레오티드의 프라이머이다.
염색체 상의 항생제 마커 제거를 위해서는 외래경로 삽입이 확인된 균주에 pCP20 플라스미드를 형질전환하여 30℃에서 배양 후 단일 콜로니를 얻었으며, 여기서 얻은 단일 콜로니들을 주형으로 하여 확인용 프라이머를 통해 PCR을 수행하고 항생제 마커가 제거된 것을 확인하였다. 확인된 콜로니는 43℃에 배양하여 온도민감성 플라스미드인 pCP20 플라스미드를 제거하여 완성된 MG1655(DE3) Δdxr ΔadhE::MVAbottom 균주를 얻었다.
상기 방법과 동일한 방법으로 MG1655(DE3) Δdxr ΔadhE::MVAbottom 균주에서 dxs 결손을 진행하여 MG1655(DE3) Δdxr /s ΔadhE::MVAbottom 균주를 얻을 수 있다. 상동성 재조합을 위해 서열번호 24과 서열번호 25의 프라이머를 이용하여, pTFKC(DPB)을 주형으로 PCR 반응을 수행하였다. 이후 확인용 프라이머는 서열번호 26과 서열번호 27, 그리고 서열번호 28의 올리고뉴클레오티드를 이용하였다.
3. 플라스미드 구축 및 배양방법( MEP 경로 결손주 )
- 염색체 내 외래 MVA 하부경로 삽입하고 MEP 상부경로 유전자를 결손시킨 균주 이용
- 상기 MEP 경로 결손주에 사용되는 플라스미드의 선별마커는 결손된 MEP 경로를 보완할 수 있는 활성화된 MVA 경로 또는 염색체의 결손된 MEP 경로 유전자가 될 수 있다. 상기 결손주에 도입할 수 있는 플라스미드의 수는 단일 또는 복수가 될 수 있으며, 단일 플라스미드 방식에서는 도입하는 단일 플라스미드에 의해 숙주의 MEP 경로 결손이 보완될 수 있어야 하고, 복수 플라스미드 방식에서는 도입하는 복수의 플라스미드가 모두 존재할 때에만 숙주의 MEP 경로 결손이 보완될 수 있도록 하는 것이다
1) 외래 MVA 경로 기반의 이소프레노이드 생합성 플라스미드
A. 단일 플라스미드 방식 ( 산탈렌 생산 예시)
- 숙주의 MEP 경로 결손을 보완하는 활성화된 MVA 경로가 단일 플라스미드에 모두 존재하는 방식으로 이 플라스미드에는 MVA 경로와 목적산물의 생합성 경로가 함께 존재하도록 구축한다.
i. 플라스미드 구축
상기 MEP 경로 결손주에서 외래 MVA 경로를 항생제 마커를 대체하는 선별마커로 사용하기 위하여, 산탈렌을 생산하는 플라스미드에 MVA 경로를 도입하여 재조합 플라스미드를 구축한다. 상기 결손주는 재조합 플라스미드가 도입될 때 생육이 가능하고 산탈렌이 생산된다.
산탈렌 생산 플라스미드인 pT-ispA-STS는 pTrc99A 벡터에 대장균(Escherichia coli)의 FPP synthase인 ispA 유전자와 Clausena lansium의 santalene synthase 유전자인 STS를 도입한 것이다. 상기 플라스미드에서 STS 유전자 뒤에 존재하는 제한효소부위 BglII와 SbfI을 이용해서 pSNAK 플라스미드에 있는 외래 MVA 경로 오페론을 클로닝하였다. pSNAK 플라스미드는 스트렙토코커스 뉴모니아(Streptococcus pneumoniae)의 mvaK1, mvaD 그리고 mvaK2와 엔테로코커스 패칼리스(Enterococcus faecalis)의 mvaE와 mvaS, 그리고 대장균(Escherichia coli)의 idi 유전자를 도입한 것이다. MVA 오페론은 양말단의 제한효소 BamHI과 SbfI으로 절단하여 pT-ispA-STS 벡터에 도입하였다. 제한효소 BamHI과 BglII는 동일한 외가닥 말단(cohesive end)을 지닌다. 최종적으로 산탈렌 생산 오페론과 MVA 경로 오페론이 함께 도입된 pTAS-NA를 구축하였다.
상기 플라스미드의 클로닝 과정에서 형질전환체의 선별은 대장균 DH5α 또는 대장균 MG1655(DE3) Δdxr ΔadhE::MVAbottom를 이용할 수 있다. 대장균 DH5α를 이용하는 경우에는 상기 구축하는 플라스미드에 존재하는 항생제 마커를 이용해서 앰피실린 플레이트에서 선별할 수 있다. 대장균 MG1655(DE3) Δdxr ΔadhE::MVAbottom를 이용하는 경우에는 숙주 대장균의 MEP 경로가 불활성화 되어서 상기에 구축하는 MVA 경로가 활성화 된 플라스미드가 도입되면 생육이 가능하기 때문에 항생제 없이도 형질전환체의 선별이 가능하다.
ii. 플라스미드 도입
상기 결손주는 재조합 플라스미드가 도입될 때 생육이 가능하고 산탈렌이 생산된다. 상기 구축된 플라스미드 pTAS-NA를 MEP 경로가 결손된 MG1655(DE3) Δdxr ΔadhE::MVAbottom 균주에 도입하여 mevalonate가 없는 플레이트에서 형질전환체를 얻음으로써, 플라스미드의 외래 MVA경로를 이용하는 재조합 균주를 선별할 수 있다. 이후 무항생제의 배지에서 배양하여 산탈렌의 생산성을 확인함으로써, MVA 경로 기반의 선별마커를 지니는 플라스미드의 유지 및 활성 능력을 확인 할 수 있다.
상기 항생제 프리 산탈렌 생산 관련 플라스미드를 갖는 재조합 균주를 무항생제 조건에서 종균배양하고, 생산배지인 2% (v/v) 글리세롤 및 0.2mM IPTG를 함유한 2YT배지(리터 당 16g 트립톤, 10g 이스트 추출물, 및 5g NaCl) 4mL와 데칸 1mL의 혼합 배지 중에서 배양한다. 배양은 길이 15 cm, 지름 25 mm의 홈이 있는 시험관에 상기 혼합 배지를 넣고 각 균주를 접종한 후, 진탕 배양기 (shaking incubator)에 약 250 rpm으로 교반하면서 30℃에서 배양한다.
iii. 배양 결과
야생형 대장균 MG155(DE3) 균주를 대조군으로 하여 새롭게 구축한 재조합 균주인 MG1655(DE3) Δdxr ΔadhE::MVAbottom에서의 산탈렌 생산량을 비교하였다. 배양 결과는 도 8과 표 3에 나타내었다.
상세히 설명하자면, MG1655(DE3)와 MG1655(DE3) ΔadhE::MVAbottom 균주에서는 항생제를 첨가하여 배양하였으며, 항생제 프리 시스템을 적용할 수 있는 MG1655(DE3) Δdxr ΔadhE::MVAbottom균주는 항생제 첨가 유무에 변화를 주어 배양을 진행하였다. 48시간의 배양 후 데칸층을 회수하여 GC를 통하여 산탈렌 생산량을 분석하였다. 그 결과, 79.8mg/L의 산탈렌을 생산한 MG1655(DE3)균주에 비하여 신규 재조합 균주인 MG1655(DE3) ΔadhE::MVAbottom 균주에서 5배 이상 높은 산탈렌 생산성(420.2mg/L)을 나타내었다. 또한, 무항생제 배양을 한 경우는 항생제를 첨가하였을 때 보다 더 높은 산탈렌 생산성(646.7mg/L)을 보였다. 이러한 산탈렌 생산량의 증가가 염색체에 추가로 도입된 MVA 하부경로에서 기인하였을 가능성을 확인하기 위하여 MG1655(DE3) ΔadhE::MVAbottom균주에서 같은 실험을 진행하였지만, 이 경우 오히려 산텔렌을 생산하지 못하는 양상을 나타내었다.
균주 (48h) MG1655(DE3) MG1655(DE3) ΔadhE::MVAbottom MG1655(DE3) Δdxr
Figure pat00001
adhE::MVAbottom
pTAS-NA pTAS-NA pTAS-NA
항생제 첨가 + + + -
균체 성장량 (OD600nm) 10.9 ± 0.5 9.1 ± 1.0 10.7 ± 0.3 12.7 ± 0.3
산탈렌 (mg/L) 79.8 ± 0.9 2.9 ± 1.3 420.2 ± 69.3 646.7 ± 6.6
B. 복수 플라스미드 방식 ( 레티노이드 생산 예시)
- 숙주의 MEP 경로 결손을 보완하는 MVA 경로가 복수의 플라스미드들에 분산되어 존재하는 방식으로 이들 복수의 플라스미드가 모두 존재하는 경우에만 MVA 경로가 활성화 될 수 있도록 하는 것이다. 즉 이들 플라스미드들에는 MVA 경로와 목적산물의 생합성 경로가 각각의 플라스미드의 크기와 구성 유전자들의 필요한 발현량을 고려해서 효율적으로 분산 배치되도록 구축한다.
i. 플라스미드 구축
MVA 하부경로가 염색체에 도입된 결손주에서 사용할 수 있는 외래 MVA경로 기반의 플라스미드 선별마커는 MVA 상부경로 유전자인 mvaE 유전자와 mvaS 유전자가 있다. mvaE 유전자는 atoB 유전자와 mvaA 유전자가 융합된 유전자로 필요 시 상기 두 유전자로 나누어 선별마커로 사용할 수 있다. 이번 실험에서는 외래 MVA 경로를 발현하는 pSNAK 플라스미드와 레티노이드를 생산하는 pT-DHBSRYbbO를 이용하여 두 플라스미드를 무항생제 조건에서 유지하는 플라스미드 시스템을 구축한다. 선별마커인 mvaE 유전자와 mvaS 유전자를 두 플라스미드에 분산 배치하여 두 플라스미드가 함께 도입되어야 숙주의 MEP 경로 결손이 보완되도록 한다. MVA 경로 선별마커 유전자 하나를 비교적 낮은 복제개수(copy number)와 lac 프로모터를 지니는 pSNAK 플라스미드에서 높은 복제개수와 trc 프로모터를 이용하는 pT-DHBSRYbbO 플라스미드로 옮겨야 하므로, 더 높은 발현량을 요구하는 mvaE 유전자를 옮겼다. 즉 mvaS 유전자가 mvaE를 제외한 MVA 경로를 발현하는 플라스미드의 선별마커로 사용되고, mvaE 유전자가 레티노이드 생산 플라스미드의 선별마커로 작용한다.
pSNAK 플라스미드의 제한효소부위 HpaI을 이용하여 mvaE 유전자를 절단 후 self-ligation으로 mvaE 유전자가 제거된 pSNAK(-E) 플라스미드를 구축했다. pSNAK(-E) 플라스미드에서 5’말단에 인산기를 지니는 서열번호 29와 서열번호 30의 프라이머를 이용한 PCR을 통해 Kanamycin 항생제 마커 유전자를 제거한 후 self-ligation으로 pSNA(-E)free를 구축했다.
pT-DHBSRYbbO는 pTrc99A 벡터에 판토에아 아글루메란스(Pantoea agglomerans) 유래의 crtE, crtB 및 crtI, 판토에아 아나나티스(Pantoea ananatis) 유래의 crtY, 대장균의 dxs 및 YbbO, 그리고 코돈최적화 된 uncultured marine bacterium 66A03 유래의 SR 유전자가 도입된 플라스미드이다. 상기 플라스미드에서 5’말단에 인산기를 지니는 서열번호 31과 32의 프라이머를 이용한 PCR을 통해 dxs 유전자를 제거하여 pT-HBSRYbbO를 구축했다.
pTEFAmvaE 플라스미드는 pTrc99A 벡터에 엔테로코커스 패칼리스(Enterococcus faecalis)의 mvaE 유전자를 도입하여 제작된 플라스미드로 이를 주형으로 하여, 서열번호 33와 34의 프라이머을 이용한 PCR을 통해 trc 프로모터와 mvaE 유전자를 함께 증폭한다. 이를 제한효소 NotI과 SalI으로 절단하여 pT-HBSRYbbO 벡터의 동일 제한효소 부위로 삽입해서, idi 유전자(ipiHP1)와 crtY 유전자 사이에 위치시킨 플라스미드 pT-HBSREYbbO를 제작했다. pT-HBSREYbbO에서 서열번호 35와 36, 그리고 서열번호 37과 38의 프라이머를 이용한 두 번의 PCR을 통해 암피실린 항생제 마커를 제거하면서 HindⅢ 제한효소 사이트를 도입한 pT-HBSREYbbOfree를 구축했다.
ii. 플라스미드 도입 및 배양 결과
① 항생제 마커를 제거하지 않은 두개의 신규 플라스미드 pSNAK(-E)와 pT-HBSREYbbO를 MG1655(DE3) Δdxr ΔadhE::MVAbottom 균주에 함께 도입하여, 무항생제 배지에서 플라스미드를 유지할 수 있는 신규 재조합 균주를 구축하였다. 기존의 pSNAK와 pT-DHBSRYbbO 플라스미드를 지니는 MG1655(DE3) 균주를 대조군으로 하여 신규 재조합 균주를 항생제 첨가 유무에 따른 레티놀 생산성을 확인하였다.
배양 결과는 표 4와 도 9에 나타내었다. 상세히 설명하면, 기존의 MG1655(DE3) 균주보다 신규 재조합 균주인 MG1655(DE3) Δdxr ΔadhE::MVAbottom에서 레티노이드의 생산성이 증가하였음을 알 수 있다. 신규 균주의 항생제 유무에 따른 배양 결과를 비교해보면, 48시간의 배양시간 동안 무항생제 조건에서 균체 생장량이 항생제 조건에 비해서 약간 낮았지만 레티노이드의 생산성은 더 높았다. 이것으로 항생제의 선택압력 없이 플라스미드가 소실되지 않고 잘 유지 및 작동을 하고 있다는 것을 알 수 있으며, 대사흐름도 균체 생장에서 레티노이드 생산 쪽으로 더 많이 유도되고 있음을 알 수 있다. 또한 항생제 유무에 따른 레티노이드의 생산량 차이는 플라스미드에서 발현되는 항생제 저항성 유전자가 항생제의 독성을 완전히 보완하지 못한다는 것을 알 수 있다. 이번 배양에서 사용한 항생제는 암피실린과 카나마이신으로 암피실린은 세포벽의 합성을 저해하는 작용을 하고, 카나마이신은 리보솜의 30s 소단위체를 저해하여 전반적인 단백질 생산을 감소시키는 역할을 한다. 따라서 무항생제 조건의 배양에서는 상기 세포에 악영향을 미치는 인자의 부재로 단백질 합성이 보다 잘 되어 생산량이 증가 하였다고 판단된다.
균주 (48h) MG1655(DE3) MG1655(DE3) Δdxr ΔadhE::MVAbottom
pSANK / pT-DHBSRYbbO pSNAK(-E) / pT-HBSREYbbO
항생제 첨가 + + -
균체 성장량 (OD600nm) 18.3 ± 0.5 11.4 ± 0.1 11.5 ± 0.2
총 레티노이드 (mg/L) 75.4 ± 0.1 83.7 ± 2 108 ± 1
② 또한 항생제 저항성 유전자들이 제거된 플라스미드들인 pSNA(-E)free와 pT-HBSREYbbOfree를 함께 MG1655(DE3) Δdxr ΔadhE::MVAbottom 균주에 형질 전환하여 무항생제 조건에서 배양을 진행하였다. 배양은 상기 레티노이드 배양조건으로 진행하였으며, 대장균의 테스트 튜브 배양 최대 시간인 72시간까지 진행하였다.
배양 결과는 표 5와 도 10에 나타내었다. 상세히 설명하면, 항생제 마커를 완전히 제거한 플라스미드가 도입된 재조합 균주에서의 레티노이드 생산속도가 가장 빠르고, 48시간에 최대 생산성을 보였으며 72시간에는 탄소원 고갈의 영향으로 약간 감소하는 것을 볼 수 있다. 따라서 플라스미드에서 항생제 마커를 제거함으로써 숙주세포의 대사적인 부담이 감소하는 것으로 추정된다.
균주 (72h) MG1655(DE3) MG1655(DE3) Δdxr ΔadhE::MVAbottom
pSANK / pT-DHBSRYbbO pSNAK(-E) / pT-HBSREYbbO pSNA(-E)free / pT-HBSREYbbOfree
항생제 첨가 + - -
균체 성장량 (OD600nm) 22.1 ± 0.03 12.9 ± 0.08 13.0 ± 0.4
총 레티노이드 (mg/L) 173.3 ± 6 218.1 ± 1 204.2 ± 3
2) 염색체의 결손된 MEP 경로 유전자를 선별 마커로 한 MEP 경로 기반의 이소프레노이드 생합성 플라스미드
A. 단일 플라스미드 방식 ( 산탈렌 생산 예시)
- 염색체의 결손된 MEP 경로 유전자를 선별 마커로 갖는 단일 플라스미드를 구축하고 숙주에 도입하는 방식으로, 이 경우에 숙주의 MEP 경로 결손은 도입된 플라스미드에 의해 보완되게 된다.
i. 플라스미드 구축
산탈렌 생산 플라스미드인 pT-ispA-STS의 산탈렌 생합성 오페론 뒤에 상기 결손주에서 결손된 MEP 경로 유전자인 dxr을 도입하여 pTAS-dxr를 구축하였다. 상세히 설명하자면, pTrc99A 플라스미드에 대장균의 dxr 유전자가 도입되어 있는 pT-dxr 플라스미드를 주형으로 하여 서열번호 39과 서열번호 40의 프라이머를 이용한 PCR을 통해 dxr 유전자의 양말단에 제한효소부위 BglII과 XhoI를 도입하면서 증폭하였다. 이를 pT-ispA-STS벡터의 STS 유전자 뒤에 존재하는 동일 제한효소부위 BglII와 SalI을 이용한 클로닝을 통해 결손된 dxr 유전자를 지니는 산탈렌 생산 플라스미드 pTAS-dxr을 구축하였다. 제한효소 SalI과 XhoI은 동일한 외가닥 말단(cohesive end)을 지닌다.
상기 플라스미드의 클로닝 과정에서 형질전환체의 선별은 대장균 DH5α 또는 대장균 MG1655(DE3) Δdxr ΔadhE::MVAbottom를 이용할 수 있다. 대장균 DH5α를 이용하는 경우에는 상기 구축하는 플라스미드에 존재하는 항생제 마커를 이용해서 앰피실린 플레이트에서 선별할 수 있다. 대장균 MG1655(DE3) Δdxr ΔadhE::MVAbottom를 이용하는 경우에는 숙주 대장균의 MEP 경로가 불활성화 되어서 상기에 구축하는 dxr 유전자가 도입된 플라스미드가 도입되면 생육이 가능하기 때문에 항생제 없이도 형질전환체의 선별이 가능하다.
ii. 플라스미드 도입
상기 구축된 플라스미드 pTAS-dxr을 MEP 경로가 차단되어 있는 MG1655(DE3) Δdxr ΔadhE::MVAbottom 균주에 도입하여 Mevalonate가 없는 플레이트에서 균주를 얻음으로써, 플라스미드의 dxr 유전자를 이용하여 내재 MEP 경로를 이용하는 균주를 선별할 수 있다. 이후 무항생제의 배지에서 배양을 진행하여 산탈렌을 생산성을 확인함으로써, 결손된 MEP 경로 유전자를 이용한 플라스미드의 유지 및 활성을 확인 할 수 있다.
상기 항생제 불필요 산탈렌 생산 플라스미드를 갖는 재조합 균주를 무항생제 조건에서 종균배양하고, 생산배지인 2% (v/v) 글리세롤 및 0.2mM IPTG를 함유한 2YT 배지(리터 당 16g 트립톤, 10g 이스트 추출물, 및 5g NaCl) 4mL와 데칸 1mL의 혼합 배지 중에서 배양한다. 배양은 길이 15 cm, 지름 25 mm의 홈이 있는 시험관에 상기 혼합 배지를 넣고 각 균주를 접종한 후, 진탕 배양기 (shaking incubator)에 약 250 rpm으로 교반하면서 30℃에서 배양한다.
iii. 배양 결과
야생형 대장균 MG155(DE3) 균주를 대조군으로 하여 신규 규축 재조합 균주인 MG1655(DE3) Δdxr ΔadhE::MVAbottom에서의 산탈렌 생산량을 비교하였다. 배양 결과는 도 11과 표 6에 나타내었다. 상세히 설명하자면, MG1655(DE3)와 MG1655(DE3)
Figure pat00002
adhE::MVAbottom 균주에서는 항생제를 첨가하여 배양하였으며, 항생제 프리 시스템을 적용할 수 있는 MG1655(DE3) Δdxr ΔadhE::MVAbottom균주는 항생제 첨가 유무에 변화를 주어 배양을 진행하였다. 48시간의 배양 후 데칸층을 회수하여 GC를 통하여 산탈렌 생산량을 분석하였다. 그 결과 MG1655(DE3)의 경우 0.6mg/L의 산탈렌을 생산한 반면, 신규구축한 MG1655(DE3) Δdxr ΔadhE::MVAbottom균주는 4.5mg/L의 산탈렌을 생산하였다. MG1655(DE3) ΔadhE::MVAbottom균주에서의 산탈렌 생산량 또한 3.4mg/L로 야생형 균주에 비하여 많은 양의 산탈렌을 생산하였다. 증가한 산탈렌의 양이 MVA 하부경로의 추가도입에서 기인하였을 가능성도 있지만, 메발로네이트를 추가 도입해주지 않았기 때문에 MVA 하부경로를 통한 IPP와 DMAPP의 증가로 보기 힘들다. adhE가 결손되면서 Acetyl-CoA가 부산물인 에탄올을 생산하지 않으면서 축적되게 되고, 이로 인해 Glucose의 해당과정의 흐름이 변하여 MEP경로의 전구물질인 G3P와 Pyruvate의 양이 증가하였을 수 있다. 따라서 산탈렌의 생산량이 증가하였을 가능성이 있다.
균주 (48h) MG1655(DE3) MG1655(DE3) ΔadhE::MVAbottom MG1655(DE3) Δdxr ΔadhE::MVAbottom
pTAS-dxr pTAS-dxr pTAS-dxr
항생제 첨가 + + + -
균체 성장량 (OD600nm) 4.9 ± 0.1 5.5 ± 0.2 5.0 ± 0.2 4.8 ± 0.2
산탈렌 (mg/L) 0.6 ± 0.0 3.4 ± 0.1 4.5 ± 0.2 3.9 ± 0.1
B. 복수 플라스미드 방식 ( 산탈렌 + 비사볼롤 생산 예시)
- 염색체의 결손된 MEP 경로 유전자들을 선별 마커들로 갖는 복수의 플라스미드를 구축하고 숙주에 도입하는 방식으로, 이 경우에 숙주의 MEP 경로 결손은 복수의 플라스미드가 모두 존재하는 경우에만 보완되도록 결손된 MEP 경로 유전자들이 각각의 플라스미드에 고르게 분산되도록 한다. 특히 숙주로 사용하는 결손주에서 염색체의 MEP 경로 유전자들은 도입하는 플라스미드의 숫자 이상으로 결손이 되어야 한다. 즉, 2개의 플라스미드를 사용하는 경우에는 최소한 2개 이상의 염색체의 MEP 경로 유전자들이 결손되어야 하고 이들 유전자들은 선별마커로 각각의 플라스미드에 분산배치 되게 된다.
i. 플라스미드 구축
산탈렌 생산 플라스미드인 pT-ispA-STS의 산탈렌 생합성 오페론 뒤에 dxs 유전자를 도입하여 pTAS-dxs를 구축하였다. 상세히 설명하자면, pTrc99A 벡터에 대장균의 dxs 유전자와 dxr 유전자가 도입된 pT-dxs/r 플라스미드를 주형으로 하여 서열번호 41과 서열번호 42의 프라이머를 이용한 PCR을 통해 dxs 유전자의 양말단에 제한효소부위 BglII와 XhoI을 도입하면서 증폭하였다. 상기 PCR 산물과 pT-ispA-STS벡터의 STS 유전자 뒤에 존재하는 동일 제한효소부위 BglII와 SalI을 이용한 클로닝을 통해 dxs 유전자를 지니는 산탈렌 생산 플라스미드 pTAS-dxs를 구축하였다. 제한효소 SalI과 XhoI은 동일한 외가닥 말단(cohesive end)을 지닌다.
마찬가지로 pTrc99A 벡터에 대장균(Escherichia coli)의 FPP Synthase인 ispA 유전자와 Matricaria recutita 의 α-bisabolol synthase 유전자인 MrBBS로 구성된 비사볼롤 생산 플라스미드인 pT-ispA-MrBBS의 비사볼롤 생합성 오페론 뒤에 dxr 유전자와 idi 유전자(b2889)를 도입하여 pTAB-idi-dxr를 구축하였다. idi 유전자는 IPP 이소머라제로 상기 구축한 플라스미드에 의하여 MEP경로가 강화되었을때, IPP와 DMAPP의 균형을 맞추어 주어 이소프레노이드의 생산성을 향상시킬 수 있다. 구축과정을 상세히 설명하자면 pT-ispA-MrBBS벡터의 MrBBS 유전자 뒤에 존재하는 제한효소부위 BglII와 SalI을 이용하여, 서열번호 43과 서열번호 44의 프라이머를 이용한 PCR을 통해 제한효소부위 BamHI과 SalI을 도입하면서 증폭한 idi 유전자와 서열번호 45과 서열번호 46의 프라이머를 이용한 PCR을 통해 제한효소부위 SalI과 HindIII를 도입하면서 증폭한 dxr 유전자를 비사볼롤 생산 플라스미드에 도입하여 pTAB-idi-dxr를 구축하였다.
상기 플라스미드의 클로닝 과정에서 형질전환체의 선별은 대장균 MG1655(DE3) Δdxr /s ΔadhE::MVAbottom를 이용할 수 있다. 대장균 MG1655(DE3) Δdxr /s ΔadhE::MVAbottom를 이용하는 경우에는 숙주 대장균의 MEP 경로가 불활성화 되어서 상기에 구축하는 dxr 유전자와 dxs 유전자가 각각 도입된 두 플라스미드가 함께 도입될 때 생육이 가능하기 때문에 항생제 없이도 형질전환체의 선별이 가능하다.
ii. 플라스미드 도입
상기 구축한 dxs 유전자를 선별마커로 지니는 플라스미드 pTAS-dxs와 dxr 유전자를 선별마커로 지니는 플라스미드 pTAB-idi-dxr를 dxr와 dxs유전자가 모두 결손되어 있는 MG1655(DE3) Δdxr /s ΔadhE::MVAbottom 균주에 함께 형질 전환하여 재조합 균주를 구축한다. 상기 재조합 균주를 무항생제 배지환경에서 배양하여 산탈렌과 비사볼롤의 생산을 확인함으로써 결손된 MEP 유전자들 각각이 복수의 플라스미드들의 선별 마커로 작동함을 확인 할 수 있다.
상기 복수 플라스미드를 갖는 재조합 균주를 무항생제 조건에서 종균배양하고, 생산배지인 2% (v/v) 글리세롤 및 0.2mM IPTG를 함유한 2YT 배지(리터 당 16g 트립톤, 10g 이스트 추출물, 및 5g NaCl) 4mL와 데칸 1mL의 혼합 배지 중에서 배양한다. 배양은 길이 15 cm, 지름 25 mm의 홈이 있는 시험관에 상기 혼합 배지를 넣고 각 균주를 접종한 후, 진탕 배양기 (shaking incubator)에 약 250 rpm으로 교반하면서 30℃에서 배양한다.
iii. 배양 결과
상기 구축한 재조합 균주를 항생제 첨가 유무에 따른 배지를 이용하여 배양을 진행하였다. 58시간의 배양 후 데칸층을 회수하여 GC를 통하여 산탈렌과 비사볼롤 생산량을 분석하였다. 배양 결과는 도 12와 표 7에 나타내었다. 상세히 설명하자면 항생제를 첨가하지 않은 배지에서도 산탈렌과 비사볼롤이 각 7.7mg/L, 40.7mg/L로 생산되었다. 이를 통해 산탈렌 생합성 유전자와 비사볼롤 생합성 유전자를 각각 지니고 있는 두 플라스미드가 모두 잘 유지되고 유전자를 발현하고 있다는 것을 알 수 있다.
균주 (58h) MG1655(DE3) Δdxs /r ΔadhE::MVAbottom
pTAS-dxs / pTAB-idi-dxr
항생제 첨가 + -
균체 성장량 (OD600nm) 10.1 ± 0.8 10.7 ± 0.7
산탈렌 (mg/L) 14.3 ± 1.6 7.7 ± 2.5
비사볼롤 (mg/L) 49.7 ± 5.0 40.7 ± 7.1
C. 외래 MVA 경로 추가 방식 ( 레티노이드 생산 예시)
- 이소프레노이드 생산에 외래 MVA 경로를 도입하는 것이 유리하다는 선행결과를 통해서 외래 MVA 경로를 플라스미드에 도입하면서도 MEP 경로 결손 유전자를 선별마커로 사용하는 방법을 제공한다. 이 경우에 숙주 균주는 MEP 경로와 MVA 경로가 모두 활성화 되기 때문에 높은 이소프레노이드 생산성을 기대할 수 있다. 다만 복수의 플라스미드를 사용하는 경우에 추가로 도입되는 외래 MVA 경로가 어느 한쪽에 집중되지 않고 사용하는 플라스미드들에 고르게 분산되도록 해서 모든 플라스미드들이 존재할 때에만 숙주의 결손된 MEP 경로가 보완이 되도록 한다.
i. 플라스미드 구축
상기 구축한 MVA 경로 유전자를 두 플라스미드에 나누어 발현하는 무항생제 레티노이드 생산 균주를 이용하여 MEP 경로의 결손 유전자를 선별마커로 사용하면서 외래 MVA경로도 함께 이용하는 재조합 플라스미드를 구축한다. 플라스미드의 사이즈가 큰 상기 mvaE 유전자가 도입된 레티노이드 생산 플라스미드 pT-HBSREYbbOfree에는 비교적 사이즈가 작은 dxr 유전자를 도입하고, 사이즈에 여유가 있는 mvaE 유전자를 제외한 MVA 경로를 발현하는 플라스미드 pSNAK(-E)에는 dxs 유전자를 도입한다.
pT-dxs/r 플라스미드를 주형으로 하여 서열번호 47과 서열번호 48의 프라이머를 이용한 PCR을 통해 MEP 상부경로 유전자인 dxs를 양 말단에 BglII와 XhoI을 도입하면서 증폭한다. pSNAK(-E)를 주형으로 하여 서열번호 49와 서열번호 50의 프라이머를 이용한 PCR로 양 말단에 BglII와 XhoI 제한효소서열을 도입하면서 벡터를 증폭한다. 두 PCR 산물을 제한효소 BglII와 XhoI으로 절단하여 벡터의 idi 유전자(b2889)와 mvaS 유전자 사이에 dxs 유전자를 삽입하여 pSNAK(-E)-dxs 구축한다. 이후 항생제 제거 프라이머를 이용한 PCR을 통해 카나마이신 항생제 저항성 유전자를 제거하여 pSNA(-E)-dxsfree를 구축한다.
pT-dxr 플라스미드를 주형으로 하여 서열번호 51과 서열번호 52의 프라이머를 이용한 PCR을 통해 MEP 상부경로 유전자인 dxr의 양 말단에 제한효소 NheI과 ScaI을 도입하면서 증폭하고, pT-HBSREYbbOfree 플라스미드를 서열번호 36과 서열번호 53, 그리고 서열번호 37과 서열번호 54의 프라이머를 이용한 두 번의 PCR을 통해 제한효소 XhoI 부위를 기점으로 각 7.1kb, 5.6kb의 두 조각으로 증폭한다. 두 조각은 xhoI으로 다시 연결하고, dxr 유전자는 제한효소 ScaI과 NheI으로 절단하여 레티노이드 오페론의 말단에 삽입하여 pT-HBSREYbbOdxrfree를 구축한다.
ii. 플라스미드 도입
MEP 경로의 dxr 유전자와 MVA 경로의 mvaE 유전자를 지니는 pT-HBSREYbbOdxrfree 플라스미드와 MEP 경로의 dxs 유전자와 mvaE 유전자를 제외한 모든 MVA 경로 유전자를 지니는 pSNA(-E)-dxsfree를 대장균 MG1655(DE3) Δdxr /s ΔadhE::MVAbottom에 함께 도입하여 MVA경로와 MEP경로를 동시에 이용하는 무항생제 레티놀 생산 재조합 균주의 레티놀 생산성을 확인한다.
iii. 배양 결과
MEP 경로 결손주에서 외래 MVA 경로만 이용하는 무항생제 레티놀 생산 재조합 균주와 함께 상기 구축된 MVA 경로와 MEP 경로를 동시에 이용하는 무항생제 레티놀 생산 재조합 균주를 무항생제 조건에서 배양하여 레티놀 생산성을 비교하였다. 배양 결과는 표 8과 도 13에 나타내었다. 상세히 설명하면, 외래 MVA 경로만으로 MEP 경로 결손을 보완하였던 레티노이드 생산 균규와 비교하여, 상기 구죽한 MVA 경로와 MEP 경로를 동시에 이용하는 무항생제 레티놀 생산 재조합 균주가 항생제 저항성 유전자를 제거 하였을 때, 보다 높은 레티놀 생산성을 나타내었다. 이를 통해 외래 MVA 경로를 추가적으로 보완하면서, 결손된 MEP 경로 유전자를 선별마커로 이용할 수 있다는 것을 확인하였다.
균주 (72h) MG1655(DE3) Δdxr ΔadhE::MVAbottom MG1655(DE3) Δdxs /r ΔadhE::MVAbottom
pSNA(-E)free / pT-HBSREYbbOfree pSNAdxs(-A) / pT-HBSRAYbbOdxr pSNAdxs(-A)free / pT-HBSRAYbbOdxrfree
항생제 첨가 - - -
균체 성장량 (OD600nm) 12.7 ± 0.24 12.6 ± 0.28 12.1 ± 0.12
총 레티노이드 (mg/L) 153.2 ± 2 124.0 ± 3 207.2 ± 1
3) 염색체의 결손된 MEP 경로 유전자를 선별 마커로 한 단백질 발현용 플라스미드
- GFP단백질을 이용하여 단백질 발현의 안정성 확인
- 단일 단백질 발현에만 국한 되는 것이 아니라 다양한 대사산물 생합성 경로 발현에도 이용할 수 있다.
i. 플라스미드 구축
녹색 형광을 발현하는 벡터 pEGFP의 EGFP 유전자 뒤에 결손주의 결손된 MEP 경로 유전자인 dxr을 도입하여 pEGFP-dxr을 구축하였다. 상세히 설명하자면, 상기 벡터의 EGFP 유전자 뒤의 제한효소부위 StuI과 SpeI 사이트를 이용하여, 서열번호 51과 서열번호 52의 프라이머를 이용한 PCR을 통해 증폭한 dxr 유전자를 도입해 주었다. 증폭된 PCR 산물은 양 말단에 제한효소부위 ScaI과 NheI이 도입되었다. 제한효소 StuI과 제한효소 ScaI은 평활말단(blunt end)를 지니며, 제한효소 SpeI과 NheI은 동일한 외가닥 말단(cohesive end)를 지닌다. 최종적으로 dxr 유전자를 지니며 녹색형광을 발현하는 플라스미드인 pEGFP-dxr을 구축한다.
ii. 플라스미드 도입
상기 구축된 pEGFP-dxr 플라스미드를 MG1655(DE3) Δdxr ΔadhE::MVAbottom 균주에 형질전환한다. 이를 항생제를 포함하지 않은 LB 평판배지에 도말하여 형광을 관찰한다.
상기 구축된 재조합 균주의 목표 단백질의 발현을 확실히 하기 위하여, 유도물질인 IPTG를 1mM 넣어주어 배양을 진행한다. 상세히 설명하자면 균주를 무항생제 조건에서 종균배양하고, 생산배지인 LB 배지 5ml에 OD600nm 0.1이 되도록 접종하여 배양한다. 배양 후 OD600nm 0.6이 되었을 시점에 1mM IPTG를 첨가해준다. 배양은 길이 15 cm, 지름 25 mm의 홈이 있는 시험관에 상기 혼합 배지를 넣고 각 균주를 접종한 후, 진탕 배양기 (shaking incubator)에 약 250 rpm으로 교반하면서 30℃에서 배양한다. 이 배양액을 채취하여 무항생제 배지 조건에서 플라스미드에 함유된 유전자의 발현의 안정성을 SDS-PAGE와 형광측정을 통하여 확인한다.
iii. 배양 결과
SDS-PAGE와 형광발현 결과는 도 14에 나타내었다. 상세히 설명하자면, 형광현미경을 통하여 녹색 형광을 관찰한 결과 항생제를 선별마커로 지니는 대조군보다 결손된 MEP경로 유전자를 플라스미드의 선별마커로 사용한 경우, 보다 선명한 형광을 나타내는 것을 확인하였다. 또한 SDS-PAGE를 통한 단백질 발현량을 비교해 보아도 대조군에 비하여 새롭게 구축한 균주의 무항생제 배양에서 강한 단백질 발현양을 나타내었다. IPTG와 같은 유도제를 처리 하지 않아도, 균주의 결손된 MEP경로 유전자의 선택압력에 의해서 플라스미드를 충분히 유지하고 유전자를 발현하고 있는 것을 확인하였다.
4. MVA 경로 변이 균주 구축 방법
1) 유산균 HMG - CoA reductase를 코딩하는 유전자( mvaA )의 불활성화
유산균의 내재하는 MVA 경로에서 율속제한효소(rate-limiting enzyme)인 Hydroxymethylglutaryl-CoA reductase (mvaA) 유전자를 Homologous recombination system을 이용하여 불활성화 하였다. Lactococcus lactis MG1363의 mvaA유전자 변이를 위해 우선 MG1363에서 mvaA 유전자 앞 1Kb 부분을 서열번호 55과 서열번호 56의 프라이머를 이용하여 중합효소연쇄반응(PCR)을 통해 증폭하고 mvaA유전자 뒤 1Kb 부분을 서열번호 57과 서열번호 58의 프라이머를 이용하여 PCR을 통해 증폭한 후 두 PCR 산물을 서열번호 59과 서열번호 60의 프라이머를 이용하여 Splicing by overhang extension(SOE) PCR을 통해 증폭하였다. 또한 pORI19 플라스미드를 서열번호 61과 서열번호 62의 프라이머를 이용하여 PCR을 통해 증폭한 후 두 PCR산물을 Ligation한 후 EC1000 수용성 세포(competent cell) 100ul에 2mm 간격의 큐벳을 통해 25 ㎌, 200Ω, 2,500V 조건에서 electroporation을 수행하였다. Electroporation한 후에 LB 배지 1ml을 첨가하고 37℃에서 30분간 배양 후 300ug/ml 에리트로마이신 항생제가 첨가된 LB 고체 평판배지에 100㎕ 도말하고 37℃에서 12시간 배양하여 pORI19-mvaA 플라스미드를 얻었다(표 2).
유산균의 수용성 세포(competent cell)를 제작하기위해 플라스미드 pVE6007를 함유한 L. lactis MG1363(pVE6007) 균주를 0.5% (v/v) glucose와 5ug/ml 클로람페니콜 항생제가 첨가된 5ml의 M17배지(1리터당 5.0g Pancreatic Digest of Casein, 5.0g Soy peptone, 5.0g Beef Extract, 2.5g Yeast Extract, 0.5g Ascorbic Acid, 0.25g Magnesium Sulfate, 10.0g Disodium-β-glycerophosphate)에 접종하고, 온도 30℃에서 16-24시간 종균배양한다. 0.5% (v/v) glucose, 0.5M sucrose, 1.5% (w/v) glycine, 5ug/ml 클로람페니콜이 첨가된 9ml의 M17 배지에 종균배양액 1ml을 접종 후 30℃에서 16-24시간 배양한다. 상기배양액 5ml을 동일한 새 배지 35ml에 접종하고 다시 30℃에서 OD600nm, 0.25까지 배양한다. 수용성 세포를 만들기 위해 상기 배양액을 얼음에서 5분간 냉각한 후에 4℃, 5,000rpm에서 15분간 원심분리해서 상층액을 제거하고, 배양액과 동일한 양의 40ml 세척완충액(0.5M sucrose, 10% glycerol)으로 2회 세척한다. 세척한 균체를 0.4ml의 세척완충액에 현탁해서 수용성 세포를 얻었다.
상기에서 얻은 수용성 세포 100ul에 pORI19-mvaA 플라스미드 3-5㎍을 2mm간격의 큐벳을 통해 25 ㎌, 200Ω, 2,500V 조건에서 electroporation을 수행한다. Electroporation한 후에 5㎍/ml 클로람페니콜이 첨가된 GM17배지 1ml을 첨가하고 30℃에서 2시간 배양 후 5㎍/ml 클로람페니콜과 5㎍/ml 에리트로마이신이 첨가된 GM17 고체 평판배지에 100㎕ 도말하여 MG1363(pORI19-mvaA, pVE6007)균주를 얻었다.
pVE6007 플라스미드 제거를 위해 상기에서 얻은 균주를 5㎍/ml 클로람페니콜과 5㎍/ml 에리트로마이신이 첨가된 5ml의 GM17 배지에 접종하여 30℃에서 16-24시간 배양 후 배양액 1ml을 cell down하여 GM17 배지 1ml로 세척 2번 진행한 후 5㎍/ml 에리트로마이신이 첨가된 10ml의 GM17 배지에 0.1% (v/v)으로 접종하여 30℃에서 16-24시간 배양한다. 동일한 배지에 0.1% (v/v)으로 접종하여 37℃에서 12시간 간격으로 계대배양을 3회 실시한 후 5ug/ml 에리트로마이신이 첨가된 GM17 고체 평판배지에 105-7 희석해 도말하여 30℃에서 16-24시간 배양한다.
Single cross over(SCO)가 일어난 균주를 PCR을 통해서 선별하였다. 200개의 콜로니를 서열번호 63와 서열번호 64의 프라이머와 함께 upstream 확인을 위한 서열번호 65 프라이머와 downstream 확인을 위한 서열번호 66 프라이머를 추가로 각각 넣어주어 총 3개의 프라이머를 이용하여 PCR을 진행하였다. Upstream에서 SCO가 일어난 경우 서열번호 63과 서열번호 65의 프라이머에 의해 확인이 가능하며 downstream에서 SCO가 일어난 경우 서열번호 64과 서열번호 66의 프라이머에 의해 확인이 가능하다. 그리고 SCO가 일어나지 않은 wild type의 경우 서열번호 63와 서열번호 64의 프라이머에 의해 확인이 가능하다. SCO가 일어난 균주를 5㎍/ml 에리트로마이신이 첨가된 5ml의 GM17 배지에 접종하여 30℃에서 16-24시간 배양 후 배양액 1ml을 cell down하여 GM17 배지 1ml로 세척 2번 진행한 후 3.3mM mevalonate가 첨가된 10ml의 GM17 배지에 0.1% (v/v)으로 접종하여 30℃에서 16-24시간 배양한다. 유전자 mvaA가 변이된 변이주의 생육을 위해서는 배지에 mevalonate를 첨가한다. 동일한 배지에 0.1% (v/v)으로 접종하여 30℃에서 12시간 간격으로 계대배양을 3회 이상 실시한 후 3.3mM mevalonate가 첨가된 GM17 고체 평판배지에 105-7 희석해 도말하여 30℃에서 16-24시간 배양한다.
Double cross over(DCO)가 일어난 균주를 PCR을 통해서 선별하였다. 400개의 콜로니를 서열번호 63와 서열번호 64의 프라이머를 이용하여 PCR을 진행하여 Lactococcus lactis MG1363ΔmvaA 균주를 구축하였다.
5. 플라스미드 구축 및 배양방법 ( MVA 경로 변이주)
- 숙주의 염색체 내 MVA 상부경로 유전자를 변이시킨 균주 이용
1) 대장균-유산균 재조합 셔틀 벡터 구축
기존 대장균-유산균 셔틀벡터 pCI372를 제한효소 AgeI과 NheI으로 절단하고, promoter, multi-cloning site, terminator를 도입한 pCIN벡터를 구축하였다(표 2).
2) 염색체의 변이된 MVA 경로 유전자를 선별 마커로 한 단백질 발현용 플라스미드
i. 플라스미드 구축
선별마커로 이용하기 위한 mvaA 유전자를 MG1363 균주로부터 서열번호 67과 서열번호 68의 프라이머를 이용하여 중합효소연쇄반응(PCR)을 통해 증폭하고 제한효소 BamHI과 XbaI를 절단한 후에 pCIN벡터의 동일한 제한효소부위에 도입해서 pCIN-mvaA벡터를 구축하였다. 녹색 형광 단백질 발현확인을 위한 EGFP 유전자를 pEGFP벡터로부터 서열번호 69과 서열번호 70의 프라이머를 이용하여 중합효소연쇄반응(PCR)을 통해 증폭하고 제한효소 SalI과 SphI으로 절단한 후에 pCIN-mvaA벡터의 동일한 제한효소부위에 도입해서 pCIN-mvaA-EGFP를 구축하였다(도 10). 상기 구축한 벡터들은 표 2에 나타내었다.
ii. 플라스미드 도입
상기 pCIN-mvaA-EGFP 재조합 플라스미드를 L. lactis MG1363ΔmvaA 균주에 형질전환하여 mvaA를 선별마커로 갖는 유산균 형질전환체를 제작한다.
pCIN-mvaA-EGFP 재조합 셔틀벡터를 L. lactis MG1363ΔmvaA 균주에 형질전환하기 위하여 수용성 세포(competent cell)를 제작한다. MG1363ΔmvaA 균주를 0.5% (v/v) glucose와 3.3mM mevalonate가 첨가된 5ml의 M17 배지에 접종하고, 온도 30℃에서 16-24시간 종균배양한다. 0.5% (v/v) glucose, 0.5M sucrose, 1.5% (w/v) glycine, 3.3mM mevalonate가 첨가된 9ml M17 배지에 종균배양액 1ml을 접종 후 30℃에서 16-24시간 배양한다. 상기 배양액 5ml을 동일한 새 배지 35ml에 접종하고 다시 30℃에서 OD600, 0.25까지 배양한다. 수용성 세포를 만들기 위해 상기 배양액을 얼음에서 5분간 냉각한 후에 온도 4℃, 5000rpm에서 15분간 원심분리해서 상층액을 제거하고, 배양액과 동일한 양의 40ml의 세척완충액 (0.5M sucrose, 10% glycerol)으로 2회 세척한다. 세척한 균체를 0.4ml의 세척완충액에 현탁해서 수용성 세포를 얻는다.
상기 얻어진 수용성 세포 100ul에 pCIN-mvaA-EGFP 플라스미드를 5ul 넣고 섞은 뒤 혼합액을 electroporation cuvette (2mm 간격)에 넣고, 25 ㎌, 200Ω, 2,500V 조건에서 electroporation을 수행한다. Electroporation 한 후에 0.5% (v/v) glucose가 첨가된 M17 배지를 1ml 첨가하고 30℃에서 1시간 배양한다. Electroporation으로 pCIN-mvaA-EGFP 플라스미드를 도입한 세포 시료를 0.5% (v/v) glucose를 첨가한 M17 고체 평판배지에 101-103 희석하여 도말하여 MG1363ΔmvaA(pCIN-mvaA-EGFP) 형질전환체를 얻는다.
iii. GFP 단백질을 이용하여 단백질 발현의 안정성 확인
상기 구축된 MG1363ΔmvaA(pCIN-mvaA-EGFP) 균주를 항생제를 포함하지 않은 0.5% (v/v) glucose를 첨가한 M17 고체 평판배지에 도말하여 형광을 관찰한다.
상기 구축된 재조합 균주의 목표 단백질의 발현을 확실히 하기 위하여 배양을 진행한다. 상세히 설명하자면 균주를 무항생제 조건에서 종균배양하고, 생산배지인 0.5% (v/v) glucose가 첨가된 M17 배지 5ml에 OD600nm 0.1이 되도록 접종하여 30℃에서 배양한다. 이 배양액을 채취하여 무항생제 배지 조건에서 플라스미드에 함유된 유전자의 발현의 안정성을 형광측정을 통하여 확인한다.
서열번호 프라이머 이름 서열번호 프라이머 이름
1 SN12Didi-F 36 RET-R
2 SN12Didi-R 37 RET-F
3 IS poxB-Ptrc-F 38 dAmp(RET)-R
4 KO poxB -R 39 dxr_1-F
5 IS ldhA-Ptrc-F 40 dxr_1-R
6 KO ldhA-R 41 dxs_1-F
7 IS adhE-Ptrc-F 42 dxs_1-R
8 KO adhE-R 43 idi-F
9 IS atoDA-Ptrc-F 44 idi-R
10 KO atoDA-R 45 dxr_3-F
11 KOpoxBCF-F 46 dxr_3-R
12 KOpoxBCF-R 47 dxs_2-F
13 KOldhACF-F 48 dxs_2-R
14 KOldhACF-R 49 dmvaE-F
15 KOadhECF-F 50 dmvaE-R
16 KOadhECF-R 51 dxr_2-F
17 KOatoDACF-F 52 dxr_2-R
18 KOatoDACF-R 53 RET_2-F
19 KO dxr-F 54 RET_2-R
20 KO dxr-R 55 mvaA u/s-Fwd
21 KOdxrCF-F 56 mvaA u/s-Rev
22 KOdxrCF-R 57 mvaA d/s-Fwd
23 dxrCF-R 58 mvaA d/s-Rev
24 KO dxs-F 59 mvaA ex-Fwd
25 KO dxs-R 60 mvaA ex-Rev
26 KOdxsCF-F 61 pORI19-Rev
27 KOdxsCF-R 62 pORI19-Fwd
28 KmCF-F 63 DCO-Fwd
29 dCmp(NA)-F 64 DCO-Rev
30 dCmp(NA)-R 65 SCO u/s-Rev
31 ddxs(RET)-F 66 SCO u/s-Fwd
32 ddxs(RET)-R 67 mvaA-Fwd
33 P1mvaE-F 68 mvaA-Rev
34 P1mvaE-R 69 EGFP-Fwd
35 dAmp(RET)-F 70 EGFP-Rev
유전자명 효소 유래 Genbank 허가번호 염기서열 아미노산서열
서열번호 서열번호
mvaE 아세틸-CoA 아세틸트란스퍼라제/하이드록시메틸글루타릴 (HMG)-CoA 리덕타제 엔테로코커스 패칼리스(Enterococcus faecalis) AF290092 104 124
mvaS HMG-CoA 신타제 엔테로코커스 패칼리스(Enterococcus faecalis) AF290092 105 125
mvaK1 메발로네이트 키나제 스트렙토코커스 뉴모니아(Streptococcus pneumoniae) AF290099 106 126
mvaK2 포스포메발로네이트 키나제 스트렙토코커스 뉴모니아 (Streptococcus pneumoniae) AF290099 107 127
mvaD 메발로네이트 디포스페이트 데카르복실라제 스트렙토코커스 뉴모니아 (Streptococcus pneumoniae) AF290099 108 128
Idi IPP 이소머라제 대장균(Escherichia coli) U00096 109 129
ipiHp1 IPP 이소포머레제 헤마토코커스 플루비알리스 (Haematococcuspluvialis) AF082325 110 130
crtE 제라닐제라닐 피로포스페이트 (GGPP) 신타제 판토에아 아글루메란스 (pantoea agglomerans) M87280 111 131
crtB 피토엔 신타제 판토에아 아글루메란스 (pantoea agglomerans) M87280 112 132
crtI 피토엔 데히드로게나
제		 판토에아 아글루메란스 (pantoea agglomerans) M87280 113 133
crtY 라이코펜-β-시클라제 판토에아 아나나티스 (pantoea ananatis) D90087 114 134
SR β-카로틴모노옥시게나제 배양되지 않은 해양 박테리아 (unculturedmarine bacterium) 66A03 blh의 대장균 코돈최적화 서열 115 135
YbbO 산화환원효소 야생형 대장균(Escherichia coli MG1655;
taxid 511145)		 1786701 116 136
dxs 1-데옥시자일룰로즈-5-포스페이트
(DXP) 신타제		 대장균(Escherichia coli) AF035440.1 117 137
dxr 1-데옥시-D-자일룰로스-50포스페이트 리덕토이소머레제(1-deoxy-D-xylulose 5-phosphate reductoisomerase) 대장균(Escherichia coli) AB013300.1 118 138
ispA 파네실 파이로포스페이트 신타제 대장균(Escherichia coli) - 119 139
STS 산탈렌 신타제 Clausena lansium - 120 140
MrBBS α-비사볼롤 신타제 Matricaria recutita KM259907.1의 대장균 코돈최적화 서열 121 141
EGFP 　enhanced green fluorescent protein Synthetic construct KX130867.1 122 142
mvaA Hydroxymethylglutaryl-CoA reductase Lactococcus lactis subsp. cremoris MG1363 (WP_011834877.1) 123 143
<110> GYEONGSANG NATIONAL UNIVERSITY OFFICE OF ACADEMY AND INDUSTRY COLLABORATION <120> MARKER COMPOSITION FOR TRANSFORMED MICROORGANISM, TRANSFORMED MICROORGANISM AND METHOD FOR TRANSFORMATION <130> 17P04058 <150> KR 10-2017-0058829 <151> 2017-05-11 <160> 143 <170> KopatentIn 2.0 <210> 1 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> SN12Didi-F <400> 1 gcgaattcag gaggtaataa tatgacaaaa aaagttggtg tcggtc 46 <210> 2 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> SN12Didi-R <400> 2 cgcctgcagg ttatttaagc tgggtaaatg cagataatc 39 <210> 3 <211> 74 <212> DNA <213> Artificial Sequence <220> <223> IS poxB-Ptrc-F <400> 3 gatgaactaa acttgttacc gttatcacat tcaggagatg gagaaccatg gtttgacagc 60 ttatcatcga ctgc 74 <210> 4 <211> 70 <212> DNA <213> Artificial Sequence <220> <223> KO poxB -R <400> 4 ccttattatg acgggaaatg ccaccctttt taccttagcc agtttgtttt tgtaggctgg 60 agctgcttcg 70 <210> 5 <211> 74 <212> DNA <213> Artificial Sequence <220> <223> IS ldhA-Ptrc-F <400> 5 atttttagta gcttaaatgt gattcaacat cactggagaa agtcttatga gtttgacagc 60 ttatcatcga ctgc 74 <210> 6 <211> 70 <212> DNA <213> Artificial Sequence <220> <223> KO ldhA-R <400> 6 ctcccctgga atgcagggga gcggcaagat taaaccagtt cgttcgggca tgtaggctgg 60 agctgcttcg 70 <210> 7 <211> 74 <212> DNA <213> Artificial Sequence <220> <223> IS adhE-Ptrc-F <400> 7 cgagcagatg atttactaaa aaagtttaac attatcagga gagcattatg gtttgacagc 60 ttatcatcga ctgc 74 <210> 8 <211> 70 <212> DNA <213> Artificial Sequence <220> <223> KO adhE-R <400> 8 ccgtttatgt tgccagacag cgctactgat taagcggatt ttttcgcttt tgtaggctgg 60 agctgcttcg 70 <210> 9 <211> 74 <212> DNA <213> Artificial Sequence <220> <223> IS atoDA-Ptrc-F <400> 9 ctattgcctg actgtaccca caacggtgta tgcaagaggg ataaaaaatg gtttgacagc 60 ttatcatcga ctgc 74 <210> 10 <211> 70 <212> DNA <213> Artificial Sequence <220> <223> KO atoDA-R <400> 10 acgcgtcata aaacgcgata tgcgaccaat cataaatcac cccgttgcgt tgtaggctgg 60 agctgcttcg 70 <210> 11 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> KOpoxBCF-F <400> 11 ttacgtactg gcctgctcct gc 22 <210> 12 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> KOpoxBCF-R <400> 12 gtcgggtaac ggtatcactg cg 22 <210> 13 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> KOldhACF-F <400> 13 tcatcagcag cgtcaacggc 20 <210> 14 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> KOldhACF-R <400> 14 cgctggtcac gggcttaccg 20 <210> 15 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> KOadhECF-F <400> 15 ccgcactgac tatactctcg 20 <210> 16 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> KOadhECF-R <400> 16 tgatcggcat tgcccagaag 20 <210> 17 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> KOatoDACF-F <400> 17 ctggcgaggt aaaaacagcc cc 22 <210> 18 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> KOatoDACF-R <400> 18 aagcgcgatc acgaatgtta gc 22 <210> 19 <211> 65 <212> DNA <213> Artificial Sequence <220> <223> KO dxr-F <400> 19 ggctggcggc gttttgcttt ttattctgtc tcaactctgg atgtttcatg ggggatccgt 60 cgacc 65 <210> 20 <211> 70 <212> DNA <213> Artificial Sequence <220> <223> KO dxr-R <400> 20 tagcgcgact ctctgtagcc ggattatcct cagcttgcga gacgcatcac tgtaggctgg 60 agctgcttcg 70 <210> 21 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> KOdxrCF-F <400> 21 ctgtgtgtga ctgtctggtc tgac 24 <210> 22 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> KOdxrCF-R <400> 22 catctgtaaa gtaaagttcg gcatagg 27 <210> 23 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> dxrCF-R <400> 23 ccattttgct caaggtcagc 20 <210> 24 <211> 70 <212> DNA <213> Artificial Sequence <220> <223> KO dxs-F <400> 24 tttcttaagc atagcaggag tggagtaggg attatgccag ccaggccttg caattcgatg 60 gggatccgtc 70 <210> 25 <211> 70 <212> DNA <213> Artificial Sequence <220> <223> KO dxs-R <400> 25 gtattaatag gcccctgatg agttttgata ttgccaaata cccgaccctg tgtaggctgg 60 agctgcttcg 70 <210> 26 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> KOdxsCF-F <400> 26 ggaaacgcga aggtcgg 17 <210> 27 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> KOdxsCF-R <400> 27 gcggactaca tcatccagcg 20 <210> 28 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> KmCF-F <400> 28 ccttgctcct gccgagaaag 20 <210> 29 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> dCmp(NA)-F <400> 29 tttttttaag gcagttattg gtg 23 <210> 30 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> dCmp(NA)-R <400> 30 agcttcctta gctcctgaaa 20 <210> 31 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> ddxs(RET)-F <400> 31 gtacccagct tggctgtttt 20 <210> 32 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> ddxs(RET)-R <400> 32 catggtgaat tcctcctgct 20 <210> 33 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> P1mvaE-F <400> 33 ttgcggccgc gctgttgaca attaatcatc cg 32 <210> 34 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> P1mvaE-R <400> 34 ttctcgagcc tgaaacggct acctaatgtg 30 <210> 35 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> dAmp(RET)-F <400> 35 cccaagcttc gtcagacccc gtagaaaag 29 <210> 36 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> RET-R <400> 36 cctcgagtta tcaggcgatt ttc 23 <210> 37 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> RET-F <400> 37 gcctgataac tcgaggaggt a 21 <210> 38 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> dAmp(RET)-R <400> 38 cccaagcttt gagcggatac atatttgaat g 31 <210> 39 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> dxr_1-F <400> 39 aaaagatctg tgtggaattg tgagcggata ac 32 <210> 40 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> dxr_1-R <400> 40 aaaactcgag tagatcagct tgcgagacgc 30 <210> 41 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> dxs_1-F <400> 41 aaaagatctg tgtggaattg tgagcggata ac 32 <210> 42 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> dxs_1-R <400> 42 aaaactcgag ttatgccagc caggccttg 29 <210> 43 <211> 46 <212> DNA <213> Artificial Sequence <220> <223> idi-f <400> 43 acggatcctg aggaggtaac gtatgcaaac ggaacacgtc atttta 46 <210> 44 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> idi-r <400> 44 tatcgtcgac tctaagatct tatttaagct gggtaaatgc ag 42 <210> 45 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> dxr_3-F <400> 45 aaagtcgacg tgtggaattg tgagcggata ac 32 <210> 46 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> dxr_3-R <400> 46 ttaaaagctt tagatcagct tgcgagacgc 30 <210> 47 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> dxs_2-F <400> 47 aaaactcgag tgtggaattg tgagcggata ac 32 <210> 48 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> dxs_2-R <400> 48 aaaagatctt tatgccagcc aggccttg 28 <210> 49 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> dmvaE-F <400> 49 aaaagatctc attaggtagc cgtttcaggc 30 <210> 50 <211> 38 <212> DNA <213> Artificial Sequence <220> <223> dmvaE-R <400> 50 aaaagatctt tctcgagact ccatggtctg tttcctcg 38 <210> 51 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> dxr_2-F <400> 51 cccagtactg tgtggaattg tgagcggata ac 32 <210> 52 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> dxr_2-R <400> 52 cccgctagct agatcagctt gcgagacgc 29 <210> 53 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> RET_2-F <400> 53 cccgctagca cagaatttgc ctggcgg 27 <210> 54 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> RET_2-R <400> 54 cccagtactc atgcctgcag ttaacgatga g 31 <210> 55 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> mvaA u/s-Fwd <400> 55 ccagcttcaa ccattaatga ag 22 <210> 56 <211> 53 <212> DNA <213> Artificial Sequence <220> <223> mvaA u/s-Rev <400> 56 caggctatta ttttctcaaa ttttttagtt aaaatttttt tctcatattt ccc 53 <210> 57 <211> 51 <212> DNA <213> Artificial Sequence <220> <223> mvaA d/s-Fwd <400> 57 gggaaatatg agaaaaaaat tttaactaaa aaatttgaga aaataatagc c 51 <210> 58 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> mvaA d/s-Rev <400> 58 gaatgacgag tggatcacc 19 <210> 59 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> mvaA ex-Fwd <400> 59 ccattaatga agtttgtggt tcag 24 <210> 60 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> mvaA ex-Rev <400> 60 cccttacatc ctctgtctca c 21 <210> 61 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> pORI19-Rev <400> 61 taccgagctc gaattcactg g 21 <210> 62 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> pORI19-Fwd <400> 62 atcctctaga gtcgacctgc 20 <210> 63 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> DCO-Fwd <400> 63 cgccttaaga acaccagtcg g 21 <210> 64 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> DCO-Rev <400> 64 gctctccgta ggtcatggc 19 <210> 65 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> SCO u/s-Rev <400> 65 acaatttcac acaggaaaca gc 22 <210> 66 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> SCO u/s-Fwd <400> 66 cccccattaa gtgccgagtg c 21 <210> 67 <211> 52 <212> DNA <213> Artificial Sequence <220> <223> mvaA-Fwd <400> 67 cgcggatcca ggaggtaata aatatgagaa aaaaatttta tcaaatgtcg cc 52 <210> 68 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> mvaA-Rev <400> 68 gtctagatta ttttctcaaa ttttttagta aattttggg 39 <210> 69 <211> 42 <212> DNA <213> Artificial Sequence <220> <223> EGFP-Fwd <400> 69 cgcgtcgaca ggaggtaata aatatggtga gcaagggcga gg 42 <210> 70 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> EGFP-Rev <400> 70 ggcatgctta cttgtacagc tcgtccatgc c 31 <210> 71 <211> 2999 <212> DNA <213> Artificial Sequence <220> <223> pSTV28 <400> 71 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc 60 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc 120 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat 180 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc 240 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg 300 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat 360 gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat 420 gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt 480 gctacgcctg aataagtgat aataagcgga tgaatggcag aaattcgaaa gcaaattcga 540 cccggtcgtc ggttcagggc agggtcgtta aatagccgct tatgtctatt gctggtttac 600 cggtttattg actaccggaa gcagtgtgac cgtgtgcttc tcaaatgcct gaggccagtt 660 tgctcaggct ctccccgtgg aggtaataat tgacgatatg atcatttatt ctgcctccca 720 gagcctgata aaaacggtta gcgcttcgtt aatacagatg taggtgttcc acagggtagc 780 cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgcttg tttcggcgtg 840 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ctgccggcac ctgtcctacg 900 agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac 960 cggaaggagc taccggacag cggtgcggac tgttgtaact cagaataaga aatgaggccg 1020 ctcatggcgt tccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca 1080 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga 1140 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt 1200 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgaat 1260 tcgagctcgg tacccgggga tcctctagag tcgacctgca ggcatgcaag cttggcactg 1320 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt 1380 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct 1440 tcccaacagt tgcgcagcct gaatggcgaa tgagcttatc gatgataagc tgtcaaacat 1500 gagaattaca acttatatcg tatggggctg acttcaggtg ctacatttga agagataaat 1560 tgcactgaaa tctagaaata ttttatctga ttaataagat gatcttcttg agatcgtttt 1620 ggtctgcgcg taatctcttg ctctgaaaac gaaaaaaccg ccttgcaggg cggtttttcg 1680 aaggttctct gagctaccaa ctctttgaac cgaggtaact ggcttggagg agcgcagtca 1740 ccaaaacttg tcctttcagt ttagccttaa ccggcgcatg acttcaagac taactcctct 1800 aaatcaatta ccagtggctg ctgccagtgg tgcttttgca tgtctttccg ggttggactc 1860 aagacgatag ttaccggata aggcgcagcg gtcggactga acggggggtt cgtgcataca 1920 gtccagcttg gagcgaactg cctacccgga actgagtgtc aggcgtggaa tgagacaaac 1980 gcggccataa cagcggaatg acaccggtaa accgaaaggc aggaacagga gagcgcacga 2040 gggagccgcc aggggaaacg cctggtatct ttatagtcct gtcgggtttc gccaccactg 2100 atttgagcgt cagatttcgt gatgcttgtc aggggggcgg agcctatgga aaaacggctt 2160 tgccgcggcc ctctcacttc cctgttaagt atcttcctgg catcttccag gaaatctccg 2220 ccccgttcgt aagccatttc cgctcgccgc agtcgaacga ccgagcgtag cgagtcagtg 2280 agcgaggaag cggaatatat cctgtatcac atattctgct gacgcaccgg tgcagccttt 2340 tttctcctgc cacatgaagc acttcactga caccctcatc agtgccaaca tagtaagcca 2400 gtatacactc cgctagcgct gatgtccggc ggtgcttttg ccgttacgca ccaccccgtc 2460 agtagctgaa caggagggac agctgataga aacagaagcc actggagcac ctcaaaaaca 2520 ccatcataca ctaaatcagt aagttggcag catcacccga cgcactttgc gccgaataaa 2580 tacctgtgac ggaagatcac ttcgcagaat aaataaatcc tggtgtccct gttgataccg 2640 ggaagccctg ggccaacttt tggcgaaaat gagacgttga tcggcacgta agaggttcca 2700 actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt 2760 caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat 2820 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata 2880 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca 2940 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattt 2999 <210> 72 <211> 4176 <212> DNA <213> Artificial Sequence <220> <223> pTrc99A <400> 72 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc 60 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc 120 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc 180 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga 240 taacaatttc acacaggaaa cagaccatgg aattcgagct cggtacccgg ggatcctcta 300 gagtcgacct gcaggcatgc aagcttggct gttttggcgg atgagagaag attttcagcc 360 tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca 420 gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg 480 atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga 540 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 600 ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg 660 tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg 720 acggatggcc tttttgcgtt tctacaaact ctttttgttt atttttctaa atacattcaa 780 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 840 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 900 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 960 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 1020 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 1080 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 1140 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 1200 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 1260 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 1320 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 1380 cgatgcctac agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 1440 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 1500 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 1560 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 1620 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 1680 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 1740 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 1800 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 1860 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 1920 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 1980 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 2040 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 2100 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 2160 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 2220 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 2280 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 2340 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 2400 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 2460 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 2520 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 2580 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 2640 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 2700 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 2760 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 2820 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 2880 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agcagatcaa 2940 ttcgcgcgcg aaggcgaagc ggcatgcatt tacgttgaca ccatcgaatg gtgcaaaacc 3000 tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa 3060 ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc 3120 gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg 3180 gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg 3240 ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg 3300 attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc 3360 ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg 3420 atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat 3480 gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc 3540 catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc 3600 gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat 3660 aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc 3720 atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg 3780 ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg 3840 cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat 3900 atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac 3960 cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca 4020 ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg 4080 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg 4140 caacgcaatt aatgtgagtt agcgcgaatt gatctg 4176 <210> 73 <211> 4620 <212> DNA <213> Artificial Sequence <220> <223> pTFKC(DPB) <400> 73 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 60 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 120 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 180 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 240 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 300 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 360 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta 420 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 480 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 540 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 600 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 660 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 720 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 780 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 840 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 900 ccttttgctc acatgtgttt gacagcttat catcgactgc acggtgcacc aatgcttctg 960 gcgtcaggca gccatcggaa gctgtggtat ggctgtgcag gtcgtaaatc actgcataat 1020 tcgtgtcgct caaggcgcac tcccgttctg gataatgttt tttgcgccga catcataacg 1080 gttctggcaa atattctgaa atgagctgtt gacaattaat catccggctc gtataatgtg 1140 tggtcacaca ggaaacagac catggaattc gagctcggta cccggggatc ctctagagtc 1200 gacctgcagg catgcaagct tggctgtttt ggcggatgag agaagatttt cagcctgata 1260 cagattaaat cagaacgcag aagcggtctg ataaaacaga atttgcctgg cggcagtagc 1320 gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt 1380 agtgtggggt ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc 1440 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag 1500 taggacaaat ccgccgggag cggatttgaa cgttgcgaag caacggcccg gagggtggcg 1560 ggcaggacgc ccgccataaa ctgccaggca tcaaattaag cagaaggcca tcctgacgga 1620 tggccttttt gcgtttctac aaactctttt tgtttatttt tctaaataca ttcaaatatg 1680 tatccgctca tgagacaata acccgataat tcgatgggga tccgtcgacc tgcagttcga 1740 agttcctatt ctctagaaag tataggaact tcagagcgct tttgaagctc acgctgccgc 1800 aagcactcag ggcgcaaggg ctgctaaagg aagcggaaca cgtagaaagc cagtccgcag 1860 aaacggtgct gaccccggat gaatgtcagc tactgggcta tctggacaag ggaaaacgca 1920 agcgcaaaga gaaagcaggt agcttgcagt gggcttacat ggcgatagct agactgggcg 1980 gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg taaggttggg 2040 aagccctgca aagtaaactg gatggctttc ttgccgccaa ggatctgatg gcgcagggga 2100 tcaagatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg 2160 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag 2220 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt 2280 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta 2340 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg 2400 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt 2460 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat 2520 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg 2580 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca 2640 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc 2700 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc 2760 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat 2820 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc 2880 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctaataaggg 2940 gatcttgaag ttcctattcc gaagttccta ttctctagaa agtataggaa cttcgaagca 3000 gctccagcct acaaggaggt aataaatatg atcaagctta tcgataccgt cgacctcgag 3060 ggggggcccg gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt 3120 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc 3180 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca 3240 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc 3300 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc 3360 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa 3420 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact 3480 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt 3540 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa 3600 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt 3660 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac 3720 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 3780 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 3840 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 3900 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 3960 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 4020 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 4080 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 4140 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 4200 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 4260 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 4320 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 4380 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 4440 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 4500 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 4560 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 4620 4620 <210> 74 <211> 4335 <212> DNA <213> Artificial Sequence <220> <223> pTFCC(DPB) <400> 74 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 60 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 120 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 180 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 240 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 300 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 360 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 420 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 480 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 540 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 600 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 660 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 720 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 780 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 840 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 900 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 960 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 1020 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 1080 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 1140 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 1200 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 1260 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 1320 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 1380 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 1440 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 1500 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 1560 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 1620 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 1680 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 1740 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 1800 ctcacatgtg tttgacagct tatcatcgac tgcacggtgc accaatgctt ctggcgtcag 1860 gcagccatcg gaagctgtgg tatggctgtg caggtcgtaa atcactgcat aattcgtgtc 1920 gctcaaggcg cactcccgtt ctggataatg ttttttgcgc cgacatcata acggttctgg 1980 caaatattct gaaatgagct gttgacaatt aatcatccgg ctcgtataat gtgtggtcac 2040 acaggaaaca gaccatggaa ttcgagctcg gtacccgggg atcctctaga gtcgacctgc 2100 aggcatgcaa gcttggctgt tttggcggat gagagaagat tttcagcctg atacagatta 2160 aatcagaacg cagaagcggt ctgataaaac agaatttgcc tggcggcagt agcgcggtgg 2220 tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg 2280 ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg 2340 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca 2400 aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg gcgggcagga 2460 cgcccgccat aaactgccag gcatcaaatt aagcagaagg ccatcctgac ggatggcctt 2520 tttgcgtttc tacaaactct ttttgtttat ttttctaaat acattcaaat atgtatccgc 2580 tcatgagaca ataacccgat aattcgatca tatgaatatc ctccttagtt cctattccga 2640 agttcctatt ctctagaaag tataggaact tcggcgcgcc tacctgtgac ggaagatcac 2700 ttcgcagaat aaataaatcc tggtgtccct gttgataccg ggaagccctg ggccaacttt 2760 tggcgaaaat gagacgttga tcggcacgta agaggttcca actttcacca taatgaaata 2820 agatcactac cgggcgtatt ttttgagttg tcgagatttt caggagctaa ggaagctaaa 2880 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa 2940 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat 3000 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt 3060 cacattcttg cccgcctgat gaatgctcat ccggaattac gtatggcaat gaaagacggt 3120 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa 3180 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat 3240 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag 3300 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg 3360 gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc 3420 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat 3480 gtcggcagat gcttaatgaa tacaacagta ctgcgatgag tggcagggcg gggcgtaagg 3540 cgcgccattt aaatgaagtt cctattccga agttcctatt ctctagaaag tataggaact 3600 tcgaagcagc tccagcctac aaggaggtaa taaatatgat caagcttatc gataccgtcg 3660 acctcgaggg ggggcccggt acccaattcg ccctatagtg agtcgtatta cgcgcgctca 3720 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc 3780 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc 3840 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta 3900 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 3960 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 4020 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 4080 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 4140 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 4200 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc 4260 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta 4320 acgcttacaa tttag 4335 <210> 75 <211> 9497 <212> DNA <213> Artificial Sequence <220> <223> pCP20 <400> 75 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt 60 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt 120 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga 180 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga 240 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt 300 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc 360 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat 420 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt 480 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg 540 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgcctgtag tgccatttac 600 ccccattcac tgccagagcc gtgagcgcag cgaactgaat gtcacgaaaa agacagcgac 660 tcaggtgcct gatggtcgga gacaaaagga atattcagcg atttgcccga gcttgcgagg 720 gtgctactta agcctttagg gttttaaggt ctgttttgta gaggagcaaa cagcgtttgc 780 gacatccttt tgtaatactg cggaactgac taaagtagtg agttatacac agggctggga 840 tctattcttt ttatcttttt ttattctttc tttattctat aaattataac cacttgaata 900 taaacaaaaa aaacacacaa aggtctagcg gaatttacag agggtctagc agaatttaca 960 agttttccag caaaggtcta gcagaattta cagataccca caactcaaag gaaaaggact 1020 agtaattatc attgactagc ccatctcaat tggtatagtg attaaaatca cctagaccaa 1080 ttgagatgta tgtctgaatt agttgttttc aaagcaaatg aactagcgat tagtcgctat 1140 gacttaacgg agcatgaaac caagctaatt ttatgctgtg tggcactact caaccccacg 1200 attgaaaacc ctacaaggaa agaacggacg gtatcgttca cttataacca atacgttcag 1260 atgatgaaca tcagtaggga aaatgcttat ggtgtattag ctaaagcaac cagagagctg 1320 atgacgagaa ctgtggaaat caggaatcct ttggttaaag gctttgagat tttccagtgg 1380 acaaactatg ccaagttctc aagcgaaaaa ttagaattag tttttagtga agagatattg 1440 ccttatcttt tccagttaaa aaaattcata aaatataatc tggaacatgt taagtctttt 1500 gaaaacaaat actctatgag gatttatgag tggttattaa aagaactaac acaaaagaaa 1560 actcacaagg caaatataga gattagcctt gatgaattta agttcatgtt aatgcttgaa 1620 aataactacc atgagtttaa aaggcttaac caatgggttt tgaaaccaat aagtaaagat 1680 ttaaacactt acagcaatat gaaattggtg gttgataagc gaggccgccc gactgatacg 1740 ttgattttcc aagttgaact agatagacaa atggatctcg taaccgaact tgagaacaac 1800 cagataaaaa tgaatggtga caaaatacca acaaccatta catcagattc ctacctacgt 1860 aacggactaa gaaaaacact acacgatgct ttaactgcaa aaattcagct caccagtttt 1920 gaggcaaaat ttttgagtga catgcaaagt aagcatgatc tcaatggttc gttctcatgg 1980 ctcacgcaaa aacaacgaac cacactagag aacatactgg ctaaatacgg aaggatctga 2040 ggttcttatg gctcttgtat ctatcagtga agcatcaaga ctaacaaaca aaagtagaac 2100 aactgttcac cgttagatat caaagggaaa actgtccata tgcacagatg aaaacggtgt 2160 aaaaaagata gatacatcag agcttttacg agtttttggt gcatttaaag ctgttcacca 2220 tgaacagatc gacaatgtaa cagatgaaca gcatgtaaca cctaatagaa caggtgaaac 2280 cagtaaaaca aagcaactag aacatgaaat tgaacacctg agacaacttg ttacagctca 2340 acagtcacac atagacagcc tgaaacaggc gatgctgctt atcgaatcaa agctgccgac 2400 aacacgggag ccagtgacgc ctcccgtggg gaaaaaatca tggcaattct ggaagaaaat 2460 agcgcctgtt tcgtttcagg caggttatca gggagtgtca gcgtcctgcg gttctccggg 2520 gcgttcgggt catgcagccc gtaatggtga tttaacagcg tctgccaagc atcaattcta 2580 ggcctgtctg cgcggtcgta gtacggctgg aggcgttttc cggtctgtag ctccatgttc 2640 ggaatgacaa aattcagctc aagccgtccc ttgtcctggt gctccaccca caggatgctg 2700 tactgatttt tttcgagacc gggcatcagt acacgctcaa agctcgccat cactttttca 2760 cgtcctcccg gcggcagctc cttctccgcg aacgacagaa caccggacgt gtatttcttc 2820 gcaaatggcg tggcatcgat gagttcccgg acttcttccg gattaccctg aagcaccgtt 2880 gcgccttcgc ggttacgctc cctccccagc aggtaatcaa ccggaccact gccaccacct 2940 tttcccctgg catgaaattt aactatcatc ccgcgccccc tgttccctga cagccagacg 3000 cagccggcgc agctcatccc cgatggccat cagtgcggcc accacctgaa cccggtcacc 3060 ggaagaccac tgcccgctgt tcaccttacg ggctgtctga ttcaggttat ttccgatggc 3120 ggccagctga cgcagtaacg gcggtgccag tgtcggcagt tttccggaac gggcaaccgg 3180 ctcccccagg cagacccgcc gcatccatac cgccagttgt ttaccctcac agcgttcaag 3240 taaccgggca tgttcatcat cagtaacccg tattgtgagc atcctctcgc gtttcatcgg 3300 tatcattacc ccatgaacag aaatccccct tacacggagg catcagtgac taaacggggt 3360 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 3420 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 3480 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 3540 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 3600 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 3660 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 3720 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 3780 cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct 3840 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 3900 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 3960 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 4020 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 4080 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac 4140 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 4200 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 4260 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 4320 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 4380 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 4440 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct 4500 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc 4560 gtcttcaaga attttataaa ccgtggagcg ggcaatactg agctgatgag caatttccgt 4620 tgcaccagtg cccttctgat gaagcgtcag cacgacgttc ctgtccacgg tacgcctgcg 4680 gccaaatttg attcctttca gctttgcttc ctgtcggccc tcattcgtgc gctctaggat 4740 cctctacgcc ggacgcatcg tggccggcat caccggcgct gaggtctgcc tcgtgaagaa 4800 ggtgttgctg actcatacca ggcctgaatc gccccatcat ccagccagaa agtgagggag 4860 ccacggttga tgagagcttt gttgtaggtg gaccagttgg tgattttgaa cttttgcttt 4920 gccacggaac ggtctgcgtt gtcgggaaga tgcgtgatct gatccttcaa ctcagcaaaa 4980 gttcgattta ttcaacaaag ccgccgtccc gtcaagtcag cgtaatgctc tgccagtgtt 5040 acaaccaatt aaccaattct gattagaaaa actcatcgag catcaaatga aactgcaatt 5100 tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag 5160 aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga 5220 ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg 5280 agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagcttc tagaggatcc 5340 ccctagagaa taggaacttc ggaataggaa cttcaaagcg tttccgaaaa cgagcgcttc 5400 cgaaaatgca acgcgagctg cgcacataca gctcactgtt cacgtcgcac ctatatctgc 5460 gtgttgcctg tatatatata tacatgagaa gaacggcata gtgcgtgttt atgcttaaat 5520 gcgtacttat atgcgtctat ttatgtagga tgaaaggtag tctagtacct cctgtgatat 5580 tatcccattc catgcggggt atcgtatgct tccttcagca ctacccttta gctgttctat 5640 atgctgccac tcctcaattg gattagtctc atccttcaat gctatcattt cctttgatat 5700 tggatcatat gcatagtacc gagaaactag tgcgaagtag tgatcaggta ttgctgttat 5760 ctgatgagta tacgttgtcc tggccacggc agaagcacgc ttatcgctcc aatttcccac 5820 aacattagtc aactccgtta ggcccttcat tgaaagaaat gaggtcatca aatgtcttcc 5880 aatgtgagat tttgggccat tttttatagc aaagattgaa taaggcgcat ttttcttcaa 5940 agctttattg tacgatctga ctaagttatc ttttaataat tggtattcct gtttattgct 6000 tgaagaattg ccggtcctat ttactcgttt taggactggt tcagaattcc tcaaaaattc 6060 atccaaatat acaagtggat cgatcctacc ccttgcgcta aagaagtata tgtgcctact 6120 aacgcttgtc tttgtctctg tcactaaaca ctggattatt actcccagat acttattttg 6180 gactaattta aatgatttcg gatcaacgtt cttaatatcg ctgaatcttc cacaattgat 6240 gaaagtagct aggaagagga attggtataa agtttttgtt tttgtaaatc tcgaagtata 6300 ctcaaacgaa tttagtattt tctcagtgat ctcccagatg ctttcaccct cacttagaag 6360 tgctttaagc atttttttac tgtggctatt tcccttatct gcttcttccg atgattcgaa 6420 ctgtaattgc aaactactta caatatcagt gatatcagat tgatgttttt gtccatagta 6480 aggaataatt gtaaattccc aagcaggaat caatttcttt aatgaggctt ccagaattgt 6540 tgctttttgc gtcttgtatt taaactggag tgatttattg acaatatcga aactcagcga 6600 attgcttatg atagtattat agctcatgaa tgtggctctc ttgattgctg ttccgttatg 6660 tgtaatcatc caacataaat aggttagttc agcagcacat aatgctattt tctcacctga 6720 aggtctttca aacctttcca caaactgacg aacaagcacc ttaggtggtg ttttacataa 6780 tataccaaat tgtggcatac aacctcctta gtacatgcaa ccattatcac cgccagaggt 6840 aaaatagtca acacgcacgg tgttagatat ttatcccttg cggtgataga tttaacgtat 6900 gagcacaaaa aagaaaccat taacacaaga gcagcttgag gacgcacgtc gccttaaagc 6960 aatttatgaa aaaaagaaaa atgaacttgg cttatcccag gaatctgtcg cagacaagat 7020 ggggatgggg cagtcaggcg ttggtgcttt atttaatggc atcaatgcat taaatgctta 7080 taacgccgca ttgcttacaa aaattctcaa agttagcgtt gaagaattta gcccttcaat 7140 cgccagagaa atctacgaga tgtatgaagc ggttagtatg cagccgtcac ttagaagtga 7200 gtatgagtac cctgtttttt ctcatgttca ggcagggatg ttctcaccta agcttagaac 7260 ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc aaaaaagcca gtgattctgc 7320 attctggctt gaggttgaag gtaattccat gaccgcacca acaggctcca agccaagctt 7380 tcctgacgga atgttaattc tcgttgaccc tgagcaggct gttgagccag gtgatttctg 7440 catagccaga cttgggggtg atgagtttac cttcaagaaa ctgatcaggg atagcggtca 7500 ggtgttttta caaccactaa acccacagta cccaatgatc ccatgcaatg agagttgttc 7560 cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag acgtttggct gatcggcaag 7620 gtgttctggt cggcgcatag ctgataacaa ttgagcaaga atcttcatcg aattagggga 7680 attttcactc ccctcagaac ataacatagt aaatggattg aattatgaag aatggttttt 7740 atgcgactta ccgcagcaaa aataaaggga aagataagcg ctcaataaac ctgtctgttt 7800 tccttaattc tctgctggct gataatcatc acctgcaggt tggctccaat tatttgtata 7860 ttcataaaat cgagcttatg catttctttc cagacttgtt caacaggcca gccattacgc 7920 tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg 7980 agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg 8040 cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat 8100 acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta 8160 cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc 8220 atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc 8280 gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga 8340 gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg cctcgagcaa 8400 gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac 8460 agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga 8520 gacacaacgt ggctttgttg aataaatcga acttttgctg agttgaagga tcagatcacg 8580 catcttcccg acaacgcaga ccgttccgtg gcaaagcaaa agttcaaaat caccaactgg 8640 tccacctaca acaaagctct catcaaccgt ggctccctca ctttctggct ggatgatggg 8700 gcgattcagg cctggtatga gtcagcaaca ccttcttcac gaggcagacc tcagcgctaa 8760 ccgtttttat caggctctgg gaggcagaat aaatgatcat atcgtcaatt attacctcca 8820 cggggagagc ctgagcaaac tggcctcagg catttgagaa gcacacggtc acactgcttc 8880 cggtagtcaa taaaccggta aaccagcaat agacataagc ggctatttaa cgaccctgcc 8940 ctgaaccgac gaccgggtcg aatttgcttt cgaatttctg ccattcatcc gcttattatc 9000 acttattcag gcgtagcacc aggcgtttaa gggcaccaat aactgcctta aaaaaattac 9060 gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg 9120 aagccatcac agacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct 9180 tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg 9240 tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca 9300 ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat 9360 atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca 9420 gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg 9480 tctttcattg ccatacg 9497 <210> 76 <211> 6329 <212> DNA <213> Artificial Sequence <220> <223> pKD46 <400> 76 catcgattta ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac 60 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat 120 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca 180 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct 240 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga 300 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat 360 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct 420 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga 480 tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg 540 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt 600 aaacccactg gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc 660 ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca 720 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt 780 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg 840 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac 900 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg 960 tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt 1020 aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca 1080 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat 1140 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat 1200 acccgttttt ttgggaattc gagctctaag gaggttataa aaaatggata ttaatactga 1260 aactgagatc aagcaaaagc attcactaac cccctttcct gttttcctaa tcagcccggc 1320 atttcgcggg cgatattttc acagctattt caggagttca gccatgaacg cttattacat 1380 tcaggatcgt cttgaggctc agagctgggc gcgtcactac cagcagctcg cccgtgaaga 1440 gaaagaggca gaactggcag acgacatgga aaaaggcctg ccccagcacc tgtttgaatc 1500 gctatgcatc gatcatttgc aacgccacgg ggccagcaaa aaatccatta cccgtgcgtt 1560 tgatgacgat gttgagtttc aggagcgcat ggcagaacac atccggtaca tggttgaaac 1620 cattgctcac caccaggttg atattgattc agaggtataa aacgaatgag tactgcactc 1680 gcaacgctgg ctgggaagct ggctgaacgt gtcggcatgg attctgtcga cccacaggaa 1740 ctgatcacca ctcttcgcca gacggcattt aaaggtgatg ccagcgatgc gcagttcatc 1800 gcattactga tcgttgccaa ccagtacggc cttaatccgt ggacgaaaga aatttacgcc 1860 tttcctgata agcagaatgg catcgttccg gtggtgggcg ttgatggctg gtcccgcatc 1920 atcaatgaaa accagcagtt tgatggcatg gactttgagc aggacaatga atcctgtaca 1980 tgccggattt accgcaagga ccgtaatcat ccgatctgcg ttaccgaatg gatggatgaa 2040 tgccgccgcg aaccattcaa aactcgcgaa ggcagagaaa tcacggggcc gtggcagtcg 2100 catcccaaac ggatgttacg tcataaagcc atgattcagt gtgcccgtct ggccttcgga 2160 tttgctggta tctatgacaa ggatgaagcc gagcgcattg tcgaaaatac tgcatacact 2220 gcagaacgtc agccggaacg cgacatcact ccggttaacg atgaaaccat gcaggagatt 2280 aacactctgc tgatcgccct ggataaaaca tgggatgacg acttattgcc gctctgttcc 2340 cagatatttc gccgcgacat tcgtgcatcg tcagaactga cacaggccga agcagtaaaa 2400 gctcttggat tcctgaaaca gaaagccgca gagcagaagg tggcagcatg acaccggaca 2460 ttatcctgca gcgtaccggg atcgatgtga gagctgtcga acagggggat gatgcgtggc 2520 acaaattacg gctcggcgtc atcaccgctt cagaagttca caacgtgata gcaaaacccc 2580 gctccggaaa gaagtggcct gacatgaaaa tgtcctactt ccacaccctg cttgctgagg 2640 tttgcaccgg tgtggctccg gaagttaacg ctaaagcact ggcctgggga aaacagtacg 2700 agaacgacgc cagaaccctg tttgaattca cttccggcgt gaatgttact gaatccccga 2760 tcatctatcg cgacgaaagt atgcgtaccg cctgctctcc cgatggttta tgcagtgacg 2820 gcaacggcct tgaactgaaa tgcccgttta cctcccggga tttcatgaag ttccggctcg 2880 gtggtttcga ggccataaag tcagcttaca tggcccaggt gcagtacagc atgtgggtga 2940 cgcgaaaaaa tgcctggtac tttgccaact atgacccgcg tatgaagcgt gaaggcctgc 3000 attatgtcgt gattgagcgg gatgaaaagt acatggcgag ttttgacgag atcgtgccgg 3060 agttcatcga aaaaatggac gaggcactgg ctgaaattgg ttttgtattt ggggagcaat 3120 ggcgatgacg catcctcacg ataatatccg ggtaggcgca atcactttcg tctactccgt 3180 tacaaagcga ggctgggtat ttcccggcct ttctgttatc cgaaatccac tgaaagcaca 3240 gcggctggct gaggagataa ataataaacg aggggctgta tgcacaaagc atcttctgtt 3300 gagttaagaa cgagtatcga gatggcacat agccttgctc aaattggaat caggtttgtg 3360 ccaataccag tagaaacaga cgaagaatcc atgggtatgg acagttttcc ctttgatatg 3420 taacggtgaa cagttgttct acttttgttt gttagtcttg atgcttcact gatagataca 3480 agagccataa gaacctcaga tccttccgta tttagccagt atgttctcta gtgtggttcg 3540 ttgtttttgc gtgagccatg agaacgaacc attgagatca tacttacttt gcatgtcact 3600 caaaaatttt gcctcaaaac tggtgagctg aatttttgca gttaaagcat cgtgtagtgt 3660 ttttcttagt ccgttacgta ggtaggaatc tgatgtaatg gttgttggta ttttgtcacc 3720 attcattttt atctggttgt tctcaagttc ggttacgaga tccatttgtc tatctagttc 3780 aacttggaaa atcaacgtat cagtcgggcg gcctcgctta tcaaccacca atttcatatt 3840 gctgtaagtg tttaaatctt tacttattgg tttcaaaacc cattggttaa gccttttaaa 3900 ctcatggtag ttattttcaa gcattaacat gaacttaaat tcatcaaggc taatctctat 3960 atttgccttg tgagttttct tttgtgttag ttcttttaat aaccactcat aaatcctcat 4020 agagtatttg ttttcaaaag acttaacatg ttccagatta tattttatga atttttttaa 4080 ctggaaaaga taaggcaata tctcttcact aaaaactaat tctaattttt cgcttgagaa 4140 cttggcatag tttgtccact ggaaaatctc aaagccttta accaaaggat tcctgatttc 4200 cacagttctc gtcatcagct ctctggttgc tttagctaat acaccataag cattttccct 4260 actgatgttc atcatctgag cgtattggtt ataagtgaac gataccgtcc gttctttcct 4320 tgtagggttt tcaatcgtgg ggttgagtag tgccacacag cataaaatta gcttggtttc 4380 atgctccgtt aagtcatagc gactaatcgc tagttcattt gctttgaaaa caactaattc 4440 agacatacat ctcaattggt ctaggtgatt ttaatcacta taccaattga gatgggctag 4500 tcaatgataa ttactagtcc ttttcctttg agttgtgggt atctgtaaat tctgctagac 4560 ctttgctgga aaacttgtaa attctgctag accctctgta aattccgcta gacctttgtg 4620 tgtttttttt gtttatattc aagtggttat aatttataga ataaagaaag aataaaaaaa 4680 gataaaaaga atagatccca gccctgtgta taactcacta ctttagtcag ttccgcagta 4740 ttacaaaagg atgtcgcaaa cgctgtttgc tcctctacaa aacagacctt aaaaccctaa 4800 aggcttaagt agcaccctcg caagctcggt tgcggccgca atcgggcaaa tcgctgaata 4860 ttccttttgt ctccgaccat caggcacctg agtcgctgtc tttttcgtga cattcagttc 4920 gctgcgctca cggctctggc agtgaatggg ggtaaatggc actacaggcg ccttttatgg 4980 attcatgcaa ggaaactacc cataatacaa gaaaagcccg tcacgggctt ctcagggcgt 5040 tttatggcgg gtctgctatg tggtgctatc tgactttttg ctgttcagca gttcctgccc 5100 tctgattttc cagtctgacc acttcggatt atcccgtgac aggtcattca gactggctaa 5160 tgcacccagt aaggcagcgg tatcatcaac ggggtctgac gctcagtgga acgaaaactc 5220 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 5280 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 5340 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 5400 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 5460 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 5520 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 5580 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 5640 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 5700 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 5760 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 5820 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 5880 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 5940 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 6000 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 6060 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 6120 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 6180 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 6240 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 6300 gcgcacattt ccccgaaaag tgccacctg 6329 <210> 77 <211> 6738 <212> DNA <213> Artificial Sequence <220> <223> pT-ispA-STS <400> 77 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc 60 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc 120 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc 180 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga 240 taacaatttc acacaggagg taataaatat ggaattcatg gactttccgc agcaactcga 300 agcctgcgtt aagcaggcca accaggcgct gagccgtttt atcgccccac tgccctttca 360 gaacactccc gtggtcgaaa ccatgcagta tggcgcatta ttaggtggta agcgcctgcg 420 acctttcctg gtttatgcca ccggtcatat gttcggcgtt agcacaaaca cgctggacgc 480 acccgctgcc gccgttgagt gtatccacgc ttactcatta attcatgatg atttaccggc 540 aatggatgat gacgatctgc gtcgcggttt gccaacctgc catgtgaagt ttggcgaagc 600 aaacgcgatt ctcgctggcg acgctttaca aacgctggcg ttctcgattt taagcgatgc 660 cgatatgccg gaagtgtcgg accgcgacag aatttcgatg atttctgaac tggcgagcgc 720 cagtggtatt gccggaatgt gcggtggtca ggcattagat ttagacgcgg aaggcaaaca 780 cgtacctctg gacgcgcttg agcgtattca tcgtcataaa accggcgcat tgattcgcgc 840 cgccgttcgc cttggtgcat taagcgccgg agataaagga cgtcgtgctc tgccggtact 900 cgacaagtat gcagagagca tcggccttgc cttccaggtt caggatgaca tcctggatgt 960 ggtgggagat actgcaacgt tgggaaaacg ccagggtgcc gaccagcaac ttggtaaaag 1020 tacctaccct gcacttctgg gtcttgagca agcccggaag aaagcccggg atctgatcga 1080 cgatgcccgt cagtcgctga aacaactggc tgaacagtca ctcgatacct cggcactgga 1140 agcgctagcg gactacatca tccagcgtaa taaataagga tccaaaaagg aggtaataaa 1200 ccatgtcaac tcaacaagtt tcatcagaga acattgttcg taacgctgcg aatttccatc 1260 ctaatatatg gggaaaccat ttcctcacat gtccttctca gacgattgat agttggactc 1320 aacagcacca caaagaactg aaagaagagg tgaggaaaat gatggtgtct gatgcaaata 1380 aacctgccca gagattgcgc ttgattgata ctgtccaaag gctaggtgtg gcttaccact 1440 ttgaaaagga gattgatgat gcattggaga aaataggtca tgaccctttt gatgataaag 1500 atgatctcta cattgtctct ctttgttttc gattgctgag gcagcatgga attaagatat 1560 catgtgatgt gtttgagaag tttaaagatg acgatggaaa attcaaggca tcattgatga 1620 atgatgttca aggcatgcta agtttatatg aggcagcaca cctagccatt cacggagaag 1680 atattttaga tgaagcaatt gttttcacga ccactcacct taagtcaacg gtatctaatt 1740 ctcctgtaaa ctctactttt gctgaacaaa tacgtcattc tctcagagtt cctctccgta 1800 aagctgtacc taggttagag tcgaggtatt tcttggatat ctattcaaga gatgatttgc 1860 acgataaaac tttgctcaat ttcgcaaagt tagactttaa tatactacaa gcaatgcacc 1920 agaaggaagc aagtgagatg accaggtggt ggagagattt tgacttcctt aaaaagctgc 1980 cttatataag agacagagtc gtggagctat atttttggat tctggtggga gtgtcttatc 2040 agcccaaatt cagcactggt agaatttttt tgtccaaaat aatatgcctt gagaccctcg 2100 tagatgatac atttgacgcc tacggtactt ttgacgagct cgcaatcttt actgaagcag 2160 ttacaagatg ggaccttggc cacagagatg cactaccaga atacatgaaa ttcattttca 2220 agacactcat tgatgtctac agtgaagctg agcaagaact ggcaaaggaa gggagatcat 2280 acagcataca ctatgcaata cgatcgttcc aagaactagt tatgaagtac ttctgcgaag 2340 ccaagtggtt aaataaaggt tatgttccga gcctggacga ttataaatca gtttcattaa 2400 gaagtatcgg ttttttaccg atagcggtag cttccttcgt tttcatgggt gatattgcaa 2460 ctaaggaggt ctttgaatgg gaaatgaata accctaagat cataatagcc gcagaaacga 2520 ttttcagatt cctggatgac atagcaggcc ataggtttga gcaaaagaga gaacatagtc 2580 catcagctat tgaatgctac aagaatcaac atggagtgtc tgaggaagag gcagttaaag 2640 cgttgtcgtt agaagttgct aatagttgga aagatataaa tgaggagctg cttctcaacc 2700 caatggctat tcctttacct ctgcttcagg tgattcttga tctctcacgt tcggccgatt 2760 ttatgtacgg taatgctcaa gatcgcttca cgcattcaac gatgatgaaa gaccaagttg 2820 atttggtgct gaaggacccc gttaagcttg acgattaaag atctgtcgac ctgcaggcat 2880 gcaagcttgg ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag 2940 aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac 3000 ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc 3060 cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac 3120 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg 3180 ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg 3240 ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg 3300 tttctacaaa ctctttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 3360 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 3420 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 3480 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 3540 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 3600 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg 3660 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 3720 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 3780 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 3840 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 3900 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct acagcaatgg 3960 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 4020 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 4080 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 4140 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 4200 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 4260 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 4320 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 4380 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 4440 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 4500 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 4560 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca 4620 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 4680 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 4740 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 4800 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 4860 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 4920 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 4980 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 5040 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 5100 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 5160 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc 5220 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta 5280 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg 5340 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct 5400 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag 5460 gttttcaccg tcatcaccga aacgcgcgag gcagcagatc aattcgcgcg cgaaggcgaa 5520 gcggcatgca tttacgttga caccatcgaa tggtgcaaaa cctttcgcgg tatggcatga 5580 tagcgcccgg aagagagtca attcagggtg gtgaatgtga aaccagtaac gttatacgat 5640 gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc 5700 cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt 5760 cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc 5820 tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat 5880 caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa 5940 gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg 6000 gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt 6060 gatgtctctg accagacacc catcaacagt attattttct cccatgaaga cggtacgcga 6120 ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca 6180 ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat 6240 caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa 6300 accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag 6360 atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc 6420 tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc 6480 atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct 6540 cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc 6600 accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 6660 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag 6720 ttagcgcgaa ttgatctg 6738 <210> 78 <211> 13899 <212> DNA <213> Artificial Sequence <220> <223> pTAS-NA <400> 78 gatcctacct gacgcttttt atcgcaactc tctactgttt ctccataccc gtttttttgg 60 gctagcagga ggaattcacc atggtaccaa tgacaaaaaa agttggtgtc ggtcaggcac 120 atagtaagat aattttaata ggggaacatg cggtcgttta cggttatcct gccatttccc 180 tgcctctttt ggaggtggag gtgacctgta aggtagttcc tgcagagagt ccttggcgcc 240 tttatgagga ggataccttg tccatggcgg tttatgcctc actggagtat ttgaatatca 300 cagaagcctg cattcgttgt gagattgact cggctatccc tgagaaacgg gggatgggtt 360 cgtcagcggc tatcagcata gcggccattc gtgcagtatt tgactactat caggctgatc 420 tgcctcatga tgtactagaa atcttggtca atcgagctga aatgattgcc catatgaatc 480 ctagtggttt ggatgctaag acctgtctta gtgaccaacc tattcgcttt atcaagaacg 540 taggatttac agaacttgag atggatttat ccgcctattt ggtgattgcc gatacgggtg 600 tttatggtca tactcgtgaa gccatccaag tggttcaaaa taagggcaag gatgccctac 660 cgtttttgca tgccttggga gaattaaccc agcaagcaga agttgcgatt tcacaaaaag 720 atgctgaagg actgggacaa atcctcagtc aagcgcattt acatttaaaa gaaattggag 780 tcagtagccc tgaggcagac tttttggttg aaacgactct tagccatggt gctctgggtg 840 ccaagatgag cggtggtggg ctaggaggtt gtatcatagc cttggtaacc aatttgacac 900 acgcacaaga actagcagaa agattagaag agaaaggagc tgttcagaca tggatagaga 960 gcctgtaaca gtacgttcct acgcaaatat tgctattatc aaatattggg gaaagaaaaa 1020 agaaaaagag atggtgcctg ctactagcag tatttctcta actttggaaa atatgtatac 1080 agagacgacc ttgtcgcctt taccagccaa tgtaacagct gacgaatttt acatcaatgg 1140 tcagctacaa aatgaggtcg agcatgccaa gatgagtaag attattgacc gttatcgtcc 1200 agctggtgag ggctttgtcc gtatcgatac tcaaaacaat atgcctacgg cagcgggcct 1260 gtcctcaagt tctagtggtt tgtccgccct ggtcaaggct tgtaatgctt atttcaagct 1320 tggattggat agaagtcagt tggcacagga agccaaattt gcctcaggct cttcttctcg 1380 gagtttttat ggaccactag gagcctggga taaggatagt ggagaaattt accctgtaga 1440 gacagacttg aaactagcta tgattatgtt ggtgctagag gacaagaaaa aaccaatctc 1500 tagccgtgac gggatgaaac tttgtgtgga aacctcgacg acttttgacg actgggttcg 1560 tcagtctgag aaggactatc aggatatgct gatttatctc aaggaaaatg attttgccaa 1620 gattggagaa ttaacggaga aaaatgctct ggctatgcat gctacgacaa agactgctag 1680 tccagccttt tcttatctga cggatgcctc ttatgaggct atggcctttg ttcgccagct 1740 tcgtgagaaa ggagaggcct gctactttac catggatgct ggtcccaatg ttaaggtctt 1800 ctgtcaggag aaagacttgg agcatttgtc agaaattttc ggtcagcgtt atcgcttgat 1860 tgtgtcaaaa acaaaggatt tgagtcaaga tgattgctgt taaaacttgc ggaaaactct 1920 attgggcagg tgaatatgct attttagagc cagggcagtt agctttgata aaggatattc 1980 ccatctatat gagggctgag attgcttttt ctgacagcta ccgtatctat tcagatatgt 2040 ttgatttcgc agtggactta aggcccaatc ctgactacag cttgattcaa gaaacgattg 2100 ctttgatggg agacttcctc gctgttcgcg gtcagaattt aagacctttt tccctaaaaa 2160 tctgtggcaa aatggaacga gaagggaaaa agtttggtct aggttctagt ggcagcgtcg 2220 ttgtcttggt tgtcaaggct ttactggctc tctataatct ttcggttgat cagaatctct 2280 tgttcaagct gactagcgct gtcttgctca agcgaggaga caatggttcc atgggcgacc 2340 ttgcctgtat tgtggcagag gatttggttc tttaccagtc atttgatcgc cagaaggcgg 2400 ctgcttggtt agaagaagaa aacttggcga cagttctgga gcgtgattgg ggatttttta 2460 tctcacaagt gaaaccaact ttagaatgtg atttcttagt gggatggacc aaggaagtgg 2520 ctgtatcgag tcacatggtc cagcaaatca agcaaaatat caatcaaaat tttttaagtt 2580 cctcaaaaga aacggtggtt tctttggtcg aagccttgga gcaggggaaa gccgaaaaag 2640 ttatcgagca agtagaagta gccagcaagc ttttagaagg cttgagtaca gatatttaca 2700 cgcctttgct tagacagttg aaagaagcca gtcaagattt gcaggccgtt gccaagagta 2760 gtggtgctgg tggtggtgac tgtggcatcg ccctgagttt tgatgcgcaa tcttctcgaa 2820 acactttaaa aaatcgttgg gccgatctgg ggattgagct cttatatcaa gaaaggatag 2880 gacatgacga caaatcgtaa tctagccccg ggaggagaga aattatgcaa acggaacacg 2940 tcattttatt gaatgcacag ggagttccca cgggtacgct ggaaaagtat gccgcacaca 3000 cggcagacac ccgcttacat ctcgcgttct ccagttggct gtttaatgcc aaaggacaat 3060 tattagttac ccgccgcgca ctgagcaaaa aagcatggcc tggcgtgtgg actaactcgg 3120 tttgtgggca cccacaactg ggagaaagca acgaagacgc agtgatccgc cgttgccgtt 3180 atgagcttgg cgtggaaatt acgcctcctg aatctatcta tcctgacttt cgctaccgcg 3240 ccaccgatcc gagtggcatt gtggaaaatg aagtgtgtcc ggtatttgcc gcacgcacca 3300 ctagtgcgtt acagatcaat gatgatgaag tgatggatta tcaatggtgt gatttagcag 3360 atgtattaca cggtattgat gccacgccgt gggcgttcag tccgtggatg gtgatgcagg 3420 cgacaaatcg cgaagccaga aaacgattat ctgcatttac ccagcttaaa taagcatgca 3480 ctagagtcga ggaaacagac catggagttg aaaacagtag ttattattga tgcattacga 3540 acaccaattg gaaaatataa aggcagctta agtcaagtaa gtgccgtaga cttaggaaca 3600 catgttacaa cacaactttt aaaaagacat tccactattt ctgaagaaat tgatcaagta 3660 atctttggaa atgttttaca agctggaaat ggccaaaatc ccgcacgaca aatagcaata 3720 aacagcggtt tatctcatga aattcccgca atgacagtta atgaggtctg cggatcagga 3780 atgaaggccg ttattttggc gaaacaattg attcaattag gagaagcgga agttttaatt 3840 gctggcggga ttgagaatat gtcccaagca cctaaattac aacgatttaa ttacgaaaca 3900 gaaagctatg atgcgccttt ttctagtatg atgtacgatg ggttaacgga tgcctttagt 3960 ggtcaagcaa tgggcttaac tgctgaaaat gtggccgaaa agtatcatgt aactagagaa 4020 gagcaagatc aattttctgt acattcacaa ttaaaagcag ctcaagcaca agcagaaggg 4080 atattcgctg acgaaatagc cccattagaa gtatcaggaa cgcttgtgga gaaagatgaa 4140 gggattcgcc ctaattcgag cgttgagaag ctaggaacgc ttaaaacagt ttttaaagaa 4200 gacggtactg taacagcagg gaatgcatca accattaatg atggggcttc tgctttgatt 4260 attgcttcac aagaatatgc cgaagcacac ggtcttcctt atttagctat tattcgagac 4320 agtgtggaag tcggtattga tccagcctat atgggaattt cgccgattaa agccattcaa 4380 aaactgttag cgcgcaatca acttactacg gaagaaattg atctgtatga aatcaacgaa 4440 gcatttgcag caacttcaat cgtggtccaa agagaactgg ctttaccaga ggaaaaggtc 4500 aacatttatg gtggcggtat ttcattaggt catgcgattg gtgccacagg tgctcgttta 4560 ttaacgagtt taagttatca attaaatcaa aaagaaaaga aatatggagt ggcttcttta 4620 tgtatcggcg gtggcttagg actcgctatg ctactagaga gacctcagca aaaaaaaaac 4680 agccgatttt atcaaatgag tcctgaggaa cgcctggctt ctcttcttaa tgaaggccag 4740 atttctgctg atacaaaaaa agaatttgaa aatacggctt tatcttcgca gattgccaat 4800 catatgattg aaaatcaaat cagtgaaaca gaagtgccga tgggcgttgg cttacattta 4860 acagtggacg aaactgatta tttggtacca atggcgacag aagagccctc agtgattgcg 4920 gctttgagta atggtgcaaa aatagcacaa ggatttaaaa cagtgaatca acaacgttta 4980 atgcgtggac aaatcgtttt ttacgatgtt gcagacgccg agtcattgat tgatgaacta 5040 caagtaagag aaacggaaat ttttcaacaa gcagagttaa gttatccatc tatcgttaaa 5100 cgcggcggcg gcttaagaga tttgcaatat cgtgcttttg atgaatcatt tgtatctgtc 5160 gactttttag tagatgttaa ggatgcaatg ggggcaaata tcgttaacgc tatgttggaa 5220 ggtgtggccg agttgttccg tgaatggttt gcggagcaaa agattttatt cagtatttta 5280 agtaattatg ccacggagtc ggttgttacg atgaaaacgg ctattccagt ttcacgttta 5340 agtaagggga gcaatggccg ggaaattgct gaaaaaattg ttttagcttc acgctatgct 5400 tcattagatc cttatcgggc agtcacgcat aacaaaggga tcatgaatgg cattgaagct 5460 gtcgttttag ctacaggaaa tgatacacgc gctgttagcg cttcttgtca tgcttttgcg 5520 gtgaaggaag gtcgctacca aggtttgact agttggacgc tggatggcga acaactaatt 5580 ggtgaaattt cagttccgct tgcgttagcc acggttggcg gtgccacaaa agtcttacct 5640 aaatctcaag cagctgctga tttgttagca gtgacggatg caaaagaact aagtcgagta 5700 gtagcggctg ttggtttggc acaaaattta gcggcgttac gggccttagt ctctgaagga 5760 attcaaaaag gacacatggc tctacaagca cgttctttag cgatgacggt cggagctact 5820 ggtaaagaag ttgaggcagt cgctcaacaa ttaaaacgtc aaaaaacgat gaaccaagac 5880 cgagccttgg ctattttaaa tgatttaaga aaacaataaa aaaacagttc agcagaaatt 5940 attctgctga actgtttttt ttcacattag gtagccgttt caggccacga gctcaggagt 6000 taaagaaatg acaattggga ttgataaaat tagttttttt gtgccccctt attatattga 6060 tatgacggca ctggctgaag ccagaaatgt agaccctgga aaatttcata ttggtattgg 6120 gcaagaccaa atggcggtga acccaatcag ccaagatatt gtgacatttg cagccaatgc 6180 cgcagaagcg atcttgacca aagaagataa agaggccatt gatatggtga ttgtcgggac 6240 tgagtccagt atcgatgagt caaaagcggc cgcagttgtc ttacatcgtt taatggggat 6300 tcaacctttc gctcgctctt tcgaaatcaa ggaagcttgt tacggagcaa cagcaggctt 6360 acagttagct aagaatcacg tagccttaca tccagataaa aaagtcttgg ttgtagcagc 6420 agatattgca aaatatggat taaattctgg cggtgagcct acacaaggag ctggggcggt 6480 tgcaatgtta gttgctagtg aaccgcgcat cttggcttta aaagaggata atgtgatgct 6540 gacgcaagat atctatgact tttggcgtcc aacaggccat ccgtatccta tggtcgatgg 6600 tcctttgtca aacgaaacct acatccaatc ttttgcccaa gtctgggatg aacataaaaa 6660 aagaaccggt cttgattttg cagattatga tgctttagcg ttccatattc cttacacaaa 6720 aatgggcaaa aaagccttat tagcaaaaat ctccgaccaa actgaagcag aacaggaacg 6780 aattttagcc cgttatgaag aaagcatcat ctatagtcgt cgcgtaggaa acttgtatac 6840 gggttcactt tatctgggac tcatttccct tttagaaaat gcaacgactt taaccgcagg 6900 caatcaaatt gggttattca gttatggttc tggtgctgtc gctgaatttt tcactggtga 6960 attagtagct ggttatcaaa atcatttaca aaaagaaact catttagcac tgctagataa 7020 tcggacagaa ctttctatcg ctgaatatga agccatgttt gcagaaactt tagacacaga 7080 tattgatcaa acgttagaag atgaattaaa atatagtatt tctgctatta ataataccgt 7140 tcgctcttat cgaaactaac tgcagcctcg acctgcaggc atgcaagctt ggctgttttg 7200 gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 7260 taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 7320 cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 7380 actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 7440 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 7500 gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 7560 caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttt 7620 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 7680 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta 7740 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag 7800 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca 7860 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta 7920 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc 7980 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc 8040 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca 8100 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc 8160 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca 8220 taccaaacga cgagcgtgac accacgatgc ctacagcaat ggcaacaacg ttgcgcaaac 8280 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg 8340 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg 8400 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg 8460 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac 8520 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc 8580 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct 8640 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 8700 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 8760 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 8820 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 8880 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 8940 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 9000 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 9060 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 9120 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 9180 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 9240 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 9300 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 9360 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg 9420 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc 9480 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc 9540 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg 9600 catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg 9660 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta 9720 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc 9780 gaaacgcgcg aggcagcaga tcaattcgcg cgcgaaggcg aagcggcatg catttacgtt 9840 gacaccatcg aatggtgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt 9900 caattcaggg tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt 9960 gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg 10020 cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa 10080 caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac 10140 gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg 10200 gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt 10260 ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt 10320 gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca 10380 cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg 10440 gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg 10500 cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg 10560 gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat 10620 gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg 10680 cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac 10740 gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc 10800 ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag 10860 ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg 10920 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc 10980 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagcgcg aattgatctg 11040 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc 11100 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc 11160 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc 11220 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga 11280 taacaatttc acacaggagg taataaatat ggaattcatg gactttccgc agcaactcga 11340 agcctgcgtt aagcaggcca accaggcgct gagccgtttt atcgccccac tgccctttca 11400 gaacactccc gtggtcgaaa ccatgcagta tggcgcatta ttaggtggta agcgcctgcg 11460 acctttcctg gtttatgcca ccggtcatat gttcggcgtt agcacaaaca cgctggacgc 11520 acccgctgcc gccgttgagt gtatccacgc ttactcatta attcatgatg atttaccggc 11580 aatggatgat gacgatctgc gtcgcggttt gccaacctgc catgtgaagt ttggcgaagc 11640 aaacgcgatt ctcgctggcg acgctttaca aacgctggcg ttctcgattt taagcgatgc 11700 cgatatgccg gaagtgtcgg accgcgacag aatttcgatg atttctgaac tggcgagcgc 11760 cagtggtatt gccggaatgt gcggtggtca ggcattagat ttagacgcgg aaggcaaaca 11820 cgtacctctg gacgcgcttg agcgtattca tcgtcataaa accggcgcat tgattcgcgc 11880 cgccgttcgc cttggtgcat taagcgccgg agataaagga cgtcgtgctc tgccggtact 11940 cgacaagtat gcagagagca tcggccttgc cttccaggtt caggatgaca tcctggatgt 12000 ggtgggagat actgcaacgt tgggaaaacg ccagggtgcc gaccagcaac ttggtaaaag 12060 tacctaccct gcacttctgg gtcttgagca agcccggaag aaagcccggg atctgatcga 12120 cgatgcccgt cagtcgctga aacaactggc tgaacagtca ctcgatacct cggcactgga 12180 agcgctagcg gactacatca tccagcgtaa taaataagga tccaaaaagg aggtaataaa 12240 ccatgtcaac tcaacaagtt tcatcagaga acattgttcg taacgctgcg aatttccatc 12300 ctaatatatg gggaaaccat ttcctcacat gtccttctca gacgattgat agttggactc 12360 aacagcacca caaagaactg aaagaagagg tgaggaaaat gatggtgtct gatgcaaata 12420 aacctgccca gagattgcgc ttgattgata ctgtccaaag gctaggtgtg gcttaccact 12480 ttgaaaagga gattgatgat gcattggaga aaataggtca tgaccctttt gatgataaag 12540 atgatctcta cattgtctct ctttgttttc gattgctgag gcagcatgga attaagatat 12600 catgtgatgt gtttgagaag tttaaagatg acgatggaaa attcaaggca tcattgatga 12660 atgatgttca aggcatgcta agtttatatg aggcagcaca cctagccatt cacggagaag 12720 atattttaga tgaagcaatt gttttcacga ccactcacct taagtcaacg gtatctaatt 12780 ctcctgtaaa ctctactttt gctgaacaaa tacgtcattc tctcagagtt cctctccgta 12840 aagctgtacc taggttagag tcgaggtatt tcttggatat ctattcaaga gatgatttgc 12900 acgataaaac tttgctcaat ttcgcaaagt tagactttaa tatactacaa gcaatgcacc 12960 agaaggaagc aagtgagatg accaggtggt ggagagattt tgacttcctt aaaaagctgc 13020 cttatataag agacagagtc gtggagctat atttttggat tctggtggga gtgtcttatc 13080 agcccaaatt cagcactggt agaatttttt tgtccaaaat aatatgcctt gagaccctcg 13140 tagatgatac atttgacgcc tacggtactt ttgacgagct cgcaatcttt actgaagcag 13200 ttacaagatg ggaccttggc cacagagatg cactaccaga atacatgaaa ttcattttca 13260 agacactcat tgatgtctac agtgaagctg agcaagaact ggcaaaggaa gggagatcat 13320 acagcataca ctatgcaata cgatcgttcc aagaactagt tatgaagtac ttctgcgaag 13380 ccaagtggtt aaataaaggt tatgttccga gcctggacga ttataaatca gtttcattaa 13440 gaagtatcgg ttttttaccg atagcggtag cttccttcgt tttcatgggt gatattgcaa 13500 ctaaggaggt ctttgaatgg gaaatgaata accctaagat cataatagcc gcagaaacga 13560 ttttcagatt cctggatgac atagcaggcc ataggtttga gcaaaagaga gaacatagtc 13620 catcagctat tgaatgctac aagaatcaac atggagtgtc tgaggaagag gcagttaaag 13680 cgttgtcgtt agaagttgct aatagttgga aagatataaa tgaggagctg cttctcaacc 13740 caatggctat tcctttacct ctgcttcagg tgattcttga tctctcacgt tcggccgatt 13800 ttatgtacgg taatgctcaa gatcgcttca cgcattcaac gatgatgaaa gaccaagttg 13860 atttggtgct gaaggacccc gttaagcttg acgattaaa 13899 <210> 79 <211> 10342 <212> DNA <213> Artificial Sequence <220> <223> pSNAK <400> 79 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat 60 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag 120 ctatgaccat gattacgaat tcgagctcgg tacccgggga tcctacctga cgctttttat 180 cgcaactctc tactgtttct ccatacccgt ttttttgggc tagcaggagg aattcaccat 240 ggtaccaatg acaaaaaaag ttggtgtcgg tcaggcacat agtaagataa ttttaatagg 300 ggaacatgcg gtcgtttacg gttatcctgc catttccctg cctcttttgg aggtggaggt 360 gacctgtaag gtagttcctg cagagagtcc ttggcgcctt tatgaggagg ataccttgtc 420 catggcggtt tatgcctcac tggagtattt gaatatcaca gaagcctgca ttcgttgtga 480 gattgactcg gctatccctg agaaacgggg gatgggttcg tcagcggcta tcagcatagc 540 ggccattcgt gcagtatttg actactatca ggctgatctg cctcatgatg tactagaaat 600 cttggtcaat cgagctgaaa tgattgccca tatgaatcct agtggtttgg atgctaagac 660 ctgtcttagt gaccaaccta ttcgctttat caagaacgta ggatttacag aacttgagat 720 ggatttatcc gcctatttgg tgattgccga tacgggtgtt tatggtcata ctcgtgaagc 780 catccaagtg gttcaaaata agggcaagga tgccctaccg tttttgcatg ccttgggaga 840 attaacccag caagcagaag ttgcgatttc acaaaaagat gctgaaggac tgggacaaat 900 cctcagtcaa gcgcatttac atttaaaaga aattggagtc agtagccctg aggcagactt 960 tttggttgaa acgactctta gccatggtgc tctgggtgcc aagatgagcg gtggtgggct 1020 aggaggttgt atcatagcct tggtaaccaa tttgacacac gcacaagaac tagcagaaag 1080 attagaagag aaaggagctg ttcagacatg gatagagagc ctgtaacagt acgttcctac 1140 gcaaatattg ctattatcaa atattgggga aagaaaaaag aaaaagagat ggtgcctgct 1200 actagcagta tttctctaac tttggaaaat atgtatacag agacgacctt gtcgccttta 1260 ccagccaatg taacagctga cgaattttac atcaatggtc agctacaaaa tgaggtcgag 1320 catgccaaga tgagtaagat tattgaccgt tatcgtccag ctggtgaggg ctttgtccgt 1380 atcgatactc aaaacaatat gcctacggca gcgggcctgt cctcaagttc tagtggtttg 1440 tccgccctgg tcaaggcttg taatgcttat ttcaagcttg gattggatag aagtcagttg 1500 gcacaggaag ccaaatttgc ctcaggctct tcttctcgga gtttttatgg accactagga 1560 gcctgggata aggatagtgg agaaatttac cctgtagaga cagacttgaa actagctatg 1620 attatgttgg tgctagagga caagaaaaaa ccaatctcta gccgtgacgg gatgaaactt 1680 tgtgtggaaa cctcgacgac ttttgacgac tgggttcgtc agtctgagaa ggactatcag 1740 gatatgctga tttatctcaa ggaaaatgat tttgccaaga ttggagaatt aacggagaaa 1800 aatgctctgg ctatgcatgc tacgacaaag actgctagtc cagccttttc ttatctgacg 1860 gatgcctctt atgaggctat ggcctttgtt cgccagcttc gtgagaaagg agaggcctgc 1920 tactttacca tggatgctgg tcccaatgtt aaggtcttct gtcaggagaa agacttggag 1980 catttgtcag aaattttcgg tcagcgttat cgcttgattg tgtcaaaaac aaaggatttg 2040 agtcaagatg attgctgtta aaacttgcgg aaaactctat tgggcaggtg aatatgctat 2100 tttagagcca gggcagttag ctttgataaa ggatattccc atctatatga gggctgagat 2160 tgctttttct gacagctacc gtatctattc agatatgttt gatttcgcag tggacttaag 2220 gcccaatcct gactacagct tgattcaaga aacgattgct ttgatgggag acttcctcgc 2280 tgttcgcggt cagaatttaa gacctttttc cctaaaaatc tgtggcaaaa tggaacgaga 2340 agggaaaaag tttggtctag gttctagtgg cagcgtcgtt gtcttggttg tcaaggcttt 2400 actggctctc tataatcttt cggttgatca gaatctcttg ttcaagctga ctagcgctgt 2460 cttgctcaag cgaggagaca atggttccat gggcgacctt gcctgtattg tggcagagga 2520 tttggttctt taccagtcat ttgatcgcca gaaggcggct gcttggttag aagaagaaaa 2580 cttggcgaca gttctggagc gtgattgggg attttttatc tcacaagtga aaccaacttt 2640 agaatgtgat ttcttagtgg gatggaccaa ggaagtggct gtatcgagtc acatggtcca 2700 gcaaatcaag caaaatatca atcaaaattt tttaagttcc tcaaaagaaa cggtggtttc 2760 tttggtcgaa gccttggagc aggggaaagc cgaaaaagtt atcgagcaag tagaagtagc 2820 cagcaagctt ttagaaggct tgagtacaga tatttacacg cctttgctta gacagttgaa 2880 agaagccagt caagatttgc aggccgttgc caagagtagt ggtgctggtg gtggtgactg 2940 tggcatcgcc ctgagttttg atgcgcaatc ttctcgaaac actttaaaaa atcgttgggc 3000 cgatctgggg attgagctct tatatcaaga aaggatagga catgacgaca aatcgtaatc 3060 tagccccggg aggagagaaa ttatgcaaac ggaacacgtc attttattga atgcacaggg 3120 agttcccacg ggtacgctgg aaaagtatgc cgcacacacg gcagacaccc gcttacatct 3180 cgcgttctcc agttggctgt ttaatgccaa aggacaatta ttagttaccc gccgcgcact 3240 gagcaaaaaa gcatggcctg gcgtgtggac taactcggtt tgtgggcacc cacaactggg 3300 agaaagcaac gaagacgcag tgatccgccg ttgccgttat gagcttggcg tggaaattac 3360 gcctcctgaa tctatctatc ctgactttcg ctaccgcgcc accgatccga gtggcattgt 3420 ggaaaatgaa gtgtgtccgg tatttgccgc acgcaccact agtgcgttac agatcaatga 3480 tgatgaagtg atggattatc aatggtgtga tttagcagat gtattacacg gtattgatgc 3540 cacgccgtgg gcgttcagtc cgtggatggt gatgcaggcg acaaatcgcg aagccagaaa 3600 acgattatct gcatttaccc agcttaaata agcatgcact agagtcgagg aaacagacca 3660 tggagttgaa aacagtagtt attattgatg cattacgaac accaattgga aaatataaag 3720 gcagcttaag tcaagtaagt gccgtagact taggaacaca tgttacaaca caacttttaa 3780 aaagacattc cactatttct gaagaaattg atcaagtaat ctttggaaat gttttacaag 3840 ctggaaatgg ccaaaatccc gcacgacaaa tagcaataaa cagcggttta tctcatgaaa 3900 ttcccgcaat gacagttaat gaggtctgcg gatcaggaat gaaggccgtt attttggcga 3960 aacaattgat tcaattagga gaagcggaag ttttaattgc tggcgggatt gagaatatgt 4020 cccaagcacc taaattacaa cgatttaatt acgaaacaga aagctatgat gcgccttttt 4080 ctagtatgat gtacgatggg ttaacggatg cctttagtgg tcaagcaatg ggcttaactg 4140 ctgaaaatgt ggccgaaaag tatcatgtaa ctagagaaga gcaagatcaa ttttctgtac 4200 attcacaatt aaaagcagct caagcacaag cagaagggat attcgctgac gaaatagccc 4260 cattagaagt atcaggaacg cttgtggaga aagatgaagg gattcgccct aattcgagcg 4320 ttgagaagct aggaacgctt aaaacagttt ttaaagaaga cggtactgta acagcaggga 4380 atgcatcaac cattaatgat ggggcttctg ctttgattat tgcttcacaa gaatatgccg 4440 aagcacacgg tcttccttat ttagctatta ttcgagacag tgtggaagtc ggtattgatc 4500 cagcctatat gggaatttcg ccgattaaag ccattcaaaa actgttagcg cgcaatcaac 4560 ttactacgga agaaattgat ctgtatgaaa tcaacgaagc atttgcagca acttcaatcg 4620 tggtccaaag agaactggct ttaccagagg aaaaggtcaa catttatggt ggcggtattt 4680 cattaggtca tgcgattggt gccacaggtg ctcgtttatt aacgagttta agttatcaat 4740 taaatcaaaa agaaaagaaa tatggagtgg cttctttatg tatcggcggt ggcttaggac 4800 tcgctatgct actagagaga cctcagcaaa aaaaaaacag ccgattttat caaatgagtc 4860 ctgaggaacg cctggcttct cttcttaatg aaggccagat ttctgctgat acaaaaaaag 4920 aatttgaaaa tacggcttta tcttcgcaga ttgccaatca tatgattgaa aatcaaatca 4980 gtgaaacaga agtgccgatg ggcgttggct tacatttaac agtggacgaa actgattatt 5040 tggtaccaat ggcgacagaa gagccctcag tgattgcggc tttgagtaat ggtgcaaaaa 5100 tagcacaagg atttaaaaca gtgaatcaac aacgtttaat gcgtggacaa atcgtttttt 5160 acgatgttgc agacgccgag tcattgattg atgaactaca agtaagagaa acggaaattt 5220 ttcaacaagc agagttaagt tatccatcta tcgttaaacg cggcggcggc ttaagagatt 5280 tgcaatatcg tgcttttgat gaatcatttg tatctgtcga ctttttagta gatgttaagg 5340 atgcaatggg ggcaaatatc gttaacgcta tgttggaagg tgtggccgag ttgttccgtg 5400 aatggtttgc ggagcaaaag attttattca gtattttaag taattatgcc acggagtcgg 5460 ttgttacgat gaaaacggct attccagttt cacgtttaag taaggggagc aatggccggg 5520 aaattgctga aaaaattgtt ttagcttcac gctatgcttc attagatcct tatcgggcag 5580 tcacgcataa caaagggatc atgaatggca ttgaagctgt cgttttagct acaggaaatg 5640 atacacgcgc tgttagcgct tcttgtcatg cttttgcggt gaaggaaggt cgctaccaag 5700 gtttgactag ttggacgctg gatggcgaac aactaattgg tgaaatttca gttccgcttg 5760 cgttagccac ggttggcggt gccacaaaag tcttacctaa atctcaagca gctgctgatt 5820 tgttagcagt gacggatgca aaagaactaa gtcgagtagt agcggctgtt ggtttggcac 5880 aaaatttagc ggcgttacgg gccttagtct ctgaaggaat tcaaaaagga cacatggctc 5940 tacaagcacg ttctttagcg atgacggtcg gagctactgg taaagaagtt gaggcagtcg 6000 ctcaacaatt aaaacgtcaa aaaacgatga accaagaccg agccttggct attttaaatg 6060 atttaagaaa acaataaaaa aacagttcag cagaaattat tctgctgaac tgtttttttt 6120 cacattaggt agccgtttca ggccacgagc tcaggagtta aagaaatgac aattgggatt 6180 gataaaatta gtttttttgt gcccccttat tatattgata tgacggcact ggctgaagcc 6240 agaaatgtag accctggaaa atttcatatt ggtattgggc aagaccaaat ggcggtgaac 6300 ccaatcagcc aagatattgt gacatttgca gccaatgccg cagaagcgat cttgaccaaa 6360 gaagataaag aggccattga tatggtgatt gtcgggactg agtccagtat cgatgagtca 6420 aaagcggccg cagttgtctt acatcgttta atggggattc aacctttcgc tcgctctttc 6480 gaaatcaagg aagcttgtta cggagcaaca gcaggcttac agttagctaa gaatcacgta 6540 gccttacatc cagataaaaa agtcttggtt gtagcagcag atattgcaaa atatggatta 6600 aattctggcg gtgagcctac acaaggagct ggggcggttg caatgttagt tgctagtgaa 6660 ccgcgcatct tggctttaaa agaggataat gtgatgctga cgcaagatat ctatgacttt 6720 tggcgtccaa caggccatcc gtatcctatg gtcgatggtc ctttgtcaaa cgaaacctac 6780 atccaatctt ttgcccaagt ctgggatgaa cataaaaaaa gaaccggtct tgattttgca 6840 gattatgatg ctttagcgtt ccatattcct tacacaaaaa tgggcaaaaa agccttatta 6900 gcaaaaatct ccgaccaaac tgaagcagaa caggaacgaa ttttagcccg ttatgaagaa 6960 agcatcatct atagtcgtcg cgtaggaaac ttgtatacgg gttcacttta tctgggactc 7020 atttcccttt tagaaaatgc aacgacttta accgcaggca atcaaattgg gttattcagt 7080 tatggttctg gtgctgtcgc tgaatttttc actggtgaat tagtagctgg ttatcaaaat 7140 catttacaaa aagaaactca tttagcactg ctagataatc ggacagaact ttctatcgct 7200 gaatatgaag ccatgtttgc agaaacttta gacacagata ttgatcaaac gttagaagat 7260 gaattaaaat atagtatttc tgctattaat aataccgttc gctcttatcg aaactaactg 7320 cagcctcgac ctgcaggcat gcaagcttgg cactggccgt cgttttacaa cgtcgtgact 7380 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct 7440 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg 7500 gcgaatgagc ttatcgatga taagctgtca aacatgagaa ttacaactta tatcgtatgg 7560 ggctgacttc aggtgctaca tttgaagaga taaattgcac tgaaatctag aaatatttta 7620 tctgattaat aagatgatct tcttgagatc gttttggtct gcgcgtaatc tcttgctctg 7680 aaaacgaaaa aaccgccttg cagggcggtt tttcgaaggt tctctgagct accaactctt 7740 tgaaccgagg taactggctt ggaggagcgc agtcaccaaa acttgtcctt tcagtttagc 7800 cttaaccggc gcatgacttc aagactaact cctctaaatc aattaccagt ggctgctgcc 7860 agtggtgctt ttgcatgtct ttccgggttg gactcaagac gatagttacc ggataaggcg 7920 cagcggtcgg actgaacggg gggttcgtgc atacagtcca gcttggagcg aactgcctac 7980 ccggaactga gtgtcaggcg tggaatgaga caaacgcggc cataacagcg gaatgacacc 8040 ggtaaaccga aaggcaggaa caggagagcg cacgagggag ccgccagggg aaacgcctgg 8100 tatctttata gtcctgtcgg gtttcgccac cactgatttg agcgtcagat ttcgtgatgc 8160 ttgtcagggg ggcggagcct atggaaaaac ggctttgccg cggccctctc acttccctgt 8220 taagtatctt cctggcatct tccaggaaat ctccgccccg ttcgtaagcc atttccgctc 8280 gccgcagtcg aacgaccgag cgtagcgagt cagtgagcga ggaagcggaa tatatcctgt 8340 atcacatatt ctgctgacgc accggtgcag ccttttttct cctgccacat gaagcacttc 8400 actgacaccc tcatcagtgc caacatagta agccagtata cactccgcta gcgctgatgt 8460 ccggcggtgc ttttgccgtt acgcaccacc ccgtcagtag ctgaacagga gggacagctg 8520 atagaaacag aagccactgg agcacctcaa aaacaccatc atacactaaa tcagtaagtt 8580 ggcagcatca cccgacgcac tttgcgccga ataaatacct gtgacggaag atcacttcgc 8640 agaataaata aatcctggtg tccctgttga taccgggaag ccctgggcca acttttggcg 8700 aaaatgagac gttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc 8760 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgag 8820 ccatattcaa cgggaaacgt cttgctctag gccgcgatta aattccaaca tggatgctga 8880 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg 8940 attgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc 9000 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc 9060 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc 9120 cgggaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga 9180 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa 9240 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga 9300 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat 9360 gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga 9420 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat 9480 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc 9540 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca 9600 gtttcatttg atgctcgatg agtttttcta aagtactgcg atgagtggca gggcggggcg 9660 taattttttt aaggcagtta ttggtgccct taaacgcctg gtgctacgcc tgaataagtg 9720 ataataagcg gatgaatggc agaaattcga aagcaaattc gacccggtcg tcggttcagg 9780 gcagggtcgt taaatagccg cttatgtcta ttgctggttt accggtttat tgactaccgg 9840 aagcagtgtg accgtgtgct tctcaaatgc ctgaggccag tttgctcagg ctctccccgt 9900 ggaggtaata attgacgata tgatcattta ttctgcctcc cagagcctga taaaaacggt 9960 tagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg 10020 cagatccgga acataatggt gcagggcgct tgtttcggcg tgggtatggt ggcaggcccc 10080 gtggccgggg gactgttggg cgctgccggc acctgtccta cgagttgcat gataaagaag 10140 acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctaccggac 10200 agcggtgcgg actgttgtaa ctcagaataa gaaatgaggc cgctcatggc gttccaatac 10260 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc 10320 ccgactggaa agcgggcagt ga 10342 <210> 80 <211> 9081 <212> DNA <213> Artificial Sequence <220> <223> pSNAK(-E) <400> 80 aacgctatgt tggaaggtgt ggccgagttg ttccgtgaat ggtttgcgga gcaaaagatt 60 ttattcagta ttttaagtaa ttatgccacg gagtcggttg ttacgatgaa aacggctatt 120 ccagtttcac gtttaagtaa ggggagcaat ggccgggaaa ttgctgaaaa aattgtttta 180 gcttcacgct atgcttcatt agatccttat cgggcagtca cgcataacaa agggatcatg 240 aatggcattg aagctgtcgt tttagctaca ggaaatgata cacgcgctgt tagcgcttct 300 tgtcatgctt ttgcggtgaa ggaaggtcgc taccaaggtt tgactagttg gacgctggat 360 ggcgaacaac taattggtga aatttcagtt ccgcttgcgt tagccacggt tggcggtgcc 420 acaaaagtct tacctaaatc tcaagcagct gctgatttgt tagcagtgac ggatgcaaaa 480 gaactaagtc gagtagtagc ggctgttggt ttggcacaaa atttagcggc gttacgggcc 540 ttagtctctg aaggaattca aaaaggacac atggctctac aagcacgttc tttagcgatg 600 acggtcggag ctactggtaa agaagttgag gcagtcgctc aacaattaaa acgtcaaaaa 660 acgatgaacc aagaccgagc cttggctatt ttaaatgatt taagaaaaca ataaaaaaac 720 agttcagcag aaattattct gctgaactgt tttttttcac attaggtagc cgtttcaggc 780 cacgagctca ggagttaaag aaatgacaat tgggattgat aaaattagtt tttttgtgcc 840 cccttattat attgatatga cggcactggc tgaagccaga aatgtagacc ctggaaaatt 900 tcatattggt attgggcaag accaaatggc ggtgaaccca atcagccaag atattgtgac 960 atttgcagcc aatgccgcag aagcgatctt gaccaaagaa gataaagagg ccattgatat 1020 ggtgattgtc gggactgagt ccagtatcga tgagtcaaaa gcggccgcag ttgtcttaca 1080 tcgtttaatg gggattcaac ctttcgctcg ctctttcgaa atcaaggaag cttgttacgg 1140 agcaacagca ggcttacagt tagctaagaa tcacgtagcc ttacatccag ataaaaaagt 1200 cttggttgta gcagcagata ttgcaaaata tggattaaat tctggcggtg agcctacaca 1260 aggagctggg gcggttgcaa tgttagttgc tagtgaaccg cgcatcttgg ctttaaaaga 1320 ggataatgtg atgctgacgc aagatatcta tgacttttgg cgtccaacag gccatccgta 1380 tcctatggtc gatggtcctt tgtcaaacga aacctacatc caatcttttg cccaagtctg 1440 ggatgaacat aaaaaaagaa ccggtcttga ttttgcagat tatgatgctt tagcgttcca 1500 tattccttac acaaaaatgg gcaaaaaagc cttattagca aaaatctccg accaaactga 1560 agcagaacag gaacgaattt tagcccgtta tgaagaaagc atcatctata gtcgtcgcgt 1620 aggaaacttg tatacgggtt cactttatct gggactcatt tcccttttag aaaatgcaac 1680 gactttaacc gcaggcaatc aaattgggtt attcagttat ggttctggtg ctgtcgctga 1740 atttttcact ggtgaattag tagctggtta tcaaaatcat ttacaaaaag aaactcattt 1800 agcactgcta gataatcgga cagaactttc tatcgctgaa tatgaagcca tgtttgcaga 1860 aactttagac acagatattg atcaaacgtt agaagatgaa ttaaaatata gtatttctgc 1920 tattaataat accgttcgct cttatcgaaa ctaactgcag cctcgacctg caggcatgca 1980 agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa 2040 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc 2100 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgagctta tcgatgataa 2160 gctgtcaaac atgagaatta caacttatat cgtatggggc tgacttcagg tgctacattt 2220 gaagagataa attgcactga aatctagaaa tattttatct gattaataag atgatcttct 2280 tgagatcgtt ttggtctgcg cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag 2340 ggcggttttt cgaaggttct ctgagctacc aactctttga accgaggtaa ctggcttgga 2400 ggagcgcagt caccaaaact tgtcctttca gtttagcctt aaccggcgca tgacttcaag 2460 actaactcct ctaaatcaat taccagtggc tgctgccagt ggtgcttttg catgtctttc 2520 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcggact gaacgggggg 2580 ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg 2640 aatgagacaa acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag 2700 gagagcgcac gagggagccg ccaggggaaa cgcctggtat ctttatagtc ctgtcgggtt 2760 tcgccaccac tgatttgagc gtcagatttc gtgatgcttg tcaggggggc ggagcctatg 2820 gaaaaacggc tttgccgcgg ccctctcact tccctgttaa gtatcttcct ggcatcttcc 2880 aggaaatctc cgccccgttc gtaagccatt tccgctcgcc gcagtcgaac gaccgagcgt 2940 agcgagtcag tgagcgagga agcggaatat atcctgtatc acatattctg ctgacgcacc 3000 ggtgcagcct tttttctcct gccacatgaa gcacttcact gacaccctca tcagtgccaa 3060 catagtaagc cagtatacac tccgctagcg ctgatgtccg gcggtgcttt tgccgttacg 3120 caccaccccg tcagtagctg aacaggaggg acagctgata gaaacagaag ccactggagc 3180 acctcaaaaa caccatcata cactaaatca gtaagttggc agcatcaccc gacgcacttt 3240 gcgccgaata aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc 3300 ctgttgatac cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg 3360 taagaggttc caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt 3420 tatcgagatt ttcaggagct aaggaagcta aaatgagcca tattcaacgg gaaacgtctt 3480 gctctaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc 3540 gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc 3600 cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg 3660 tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta 3720 ctcctgatga tgcatggtta ctcaccactg cgatccccgg gaaaacagca ttccaggtat 3780 tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc 3840 ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg 3900 ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc 3960 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac 4020 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga 4080 aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg 4140 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa 4200 aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt 4260 ttttctaaag tactgcgatg agtggcaggg cggggcgtaa tttttttaag gcagttattg 4320 gtgcccttaa acgcctggtg ctacgcctga ataagtgata ataagcggat gaatggcaga 4380 aattcgaaag caaattcgac ccggtcgtcg gttcagggca gggtcgttaa atagccgctt 4440 atgtctattg ctggtttacc ggtttattga ctaccggaag cagtgtgacc gtgtgcttct 4500 caaatgcctg aggccagttt gctcaggctc tccccgtgga ggtaataatt gacgatatga 4560 tcatttattc tgcctcccag agcctgataa aaacggttag cgcttcgtta atacagatgt 4620 aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca 4680 gggcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc 4740 tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat 4800 agtcatgccc cgcgcccacc ggaaggagct accggacagc ggtgcggact gttgtaactc 4860 agaataagaa atgaggccgc tcatggcgtt ccaatacgca aaccgcctct ccccgcgcgt 4920 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag 4980 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg 5040 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc 5100 tatgaccatg attacgaatt cgagctcggt acccggggat cctacctgac gctttttatc 5160 gcaactctct actgtttctc catacccgtt tttttgggct agcaggagga attcaccatg 5220 gtaccaatga caaaaaaagt tggtgtcggt caggcacata gtaagataat tttaataggg 5280 gaacatgcgg tcgtttacgg ttatcctgcc atttccctgc ctcttttgga ggtggaggtg 5340 acctgtaagg tagttcctgc agagagtcct tggcgccttt atgaggagga taccttgtcc 5400 atggcggttt atgcctcact ggagtatttg aatatcacag aagcctgcat tcgttgtgag 5460 attgactcgg ctatccctga gaaacggggg atgggttcgt cagcggctat cagcatagcg 5520 gccattcgtg cagtatttga ctactatcag gctgatctgc ctcatgatgt actagaaatc 5580 ttggtcaatc gagctgaaat gattgcccat atgaatccta gtggtttgga tgctaagacc 5640 tgtcttagtg accaacctat tcgctttatc aagaacgtag gatttacaga acttgagatg 5700 gatttatccg cctatttggt gattgccgat acgggtgttt atggtcatac tcgtgaagcc 5760 atccaagtgg ttcaaaataa gggcaaggat gccctaccgt ttttgcatgc cttgggagaa 5820 ttaacccagc aagcagaagt tgcgatttca caaaaagatg ctgaaggact gggacaaatc 5880 ctcagtcaag cgcatttaca tttaaaagaa attggagtca gtagccctga ggcagacttt 5940 ttggttgaaa cgactcttag ccatggtgct ctgggtgcca agatgagcgg tggtgggcta 6000 ggaggttgta tcatagcctt ggtaaccaat ttgacacacg cacaagaact agcagaaaga 6060 ttagaagaga aaggagctgt tcagacatgg atagagagcc tgtaacagta cgttcctacg 6120 caaatattgc tattatcaaa tattggggaa agaaaaaaga aaaagagatg gtgcctgcta 6180 ctagcagtat ttctctaact ttggaaaata tgtatacaga gacgaccttg tcgcctttac 6240 cagccaatgt aacagctgac gaattttaca tcaatggtca gctacaaaat gaggtcgagc 6300 atgccaagat gagtaagatt attgaccgtt atcgtccagc tggtgagggc tttgtccgta 6360 tcgatactca aaacaatatg cctacggcag cgggcctgtc ctcaagttct agtggtttgt 6420 ccgccctggt caaggcttgt aatgcttatt tcaagcttgg attggataga agtcagttgg 6480 cacaggaagc caaatttgcc tcaggctctt cttctcggag tttttatgga ccactaggag 6540 cctgggataa ggatagtgga gaaatttacc ctgtagagac agacttgaaa ctagctatga 6600 ttatgttggt gctagaggac aagaaaaaac caatctctag ccgtgacggg atgaaacttt 6660 gtgtggaaac ctcgacgact tttgacgact gggttcgtca gtctgagaag gactatcagg 6720 atatgctgat ttatctcaag gaaaatgatt ttgccaagat tggagaatta acggagaaaa 6780 atgctctggc tatgcatgct acgacaaaga ctgctagtcc agccttttct tatctgacgg 6840 atgcctctta tgaggctatg gcctttgttc gccagcttcg tgagaaagga gaggcctgct 6900 actttaccat ggatgctggt cccaatgtta aggtcttctg tcaggagaaa gacttggagc 6960 atttgtcaga aattttcggt cagcgttatc gcttgattgt gtcaaaaaca aaggatttga 7020 gtcaagatga ttgctgttaa aacttgcgga aaactctatt gggcaggtga atatgctatt 7080 ttagagccag ggcagttagc tttgataaag gatattccca tctatatgag ggctgagatt 7140 gctttttctg acagctaccg tatctattca gatatgtttg atttcgcagt ggacttaagg 7200 cccaatcctg actacagctt gattcaagaa acgattgctt tgatgggaga cttcctcgct 7260 gttcgcggtc agaatttaag acctttttcc ctaaaaatct gtggcaaaat ggaacgagaa 7320 gggaaaaagt ttggtctagg ttctagtggc agcgtcgttg tcttggttgt caaggcttta 7380 ctggctctct ataatctttc ggttgatcag aatctcttgt tcaagctgac tagcgctgtc 7440 ttgctcaagc gaggagacaa tggttccatg ggcgaccttg cctgtattgt ggcagaggat 7500 ttggttcttt accagtcatt tgatcgccag aaggcggctg cttggttaga agaagaaaac 7560 ttggcgacag ttctggagcg tgattgggga ttttttatct cacaagtgaa accaacttta 7620 gaatgtgatt tcttagtggg atggaccaag gaagtggctg tatcgagtca catggtccag 7680 caaatcaagc aaaatatcaa tcaaaatttt ttaagttcct caaaagaaac ggtggtttct 7740 ttggtcgaag ccttggagca ggggaaagcc gaaaaagtta tcgagcaagt agaagtagcc 7800 agcaagcttt tagaaggctt gagtacagat atttacacgc ctttgcttag acagttgaaa 7860 gaagccagtc aagatttgca ggccgttgcc aagagtagtg gtgctggtgg tggtgactgt 7920 ggcatcgccc tgagttttga tgcgcaatct tctcgaaaca ctttaaaaaa tcgttgggcc 7980 gatctgggga ttgagctctt atatcaagaa aggataggac atgacgacaa atcgtaatct 8040 agccccggga ggagagaaat tatgcaaacg gaacacgtca ttttattgaa tgcacaggga 8100 gttcccacgg gtacgctgga aaagtatgcc gcacacacgg cagacacccg cttacatctc 8160 gcgttctcca gttggctgtt taatgccaaa ggacaattat tagttacccg ccgcgcactg 8220 agcaaaaaag catggcctgg cgtgtggact aactcggttt gtgggcaccc acaactggga 8280 gaaagcaacg aagacgcagt gatccgccgt tgccgttatg agcttggcgt ggaaattacg 8340 cctcctgaat ctatctatcc tgactttcgc taccgcgcca ccgatccgag tggcattgtg 8400 gaaaatgaag tgtgtccggt atttgccgca cgcaccacta gtgcgttaca gatcaatgat 8460 gatgaagtga tggattatca atggtgtgat ttagcagatg tattacacgg tattgatgcc 8520 acgccgtggg cgttcagtcc gtggatggtg atgcaggcga caaatcgcga agccagaaaa 8580 cgattatctg catttaccca gcttaaataa gcatgcacta gagtcgagga aacagaccat 8640 ggagttgaaa acagtagtta ttattgatgc attacgaaca ccaattggaa aatataaagg 8700 cagcttaagt caagtaagtg ccgtagactt aggaacacat gttacaacac aacttttaaa 8760 aagacattcc actatttctg aagaaattga tcaagtaatc tttggaaatg ttttacaagc 8820 tggaaatggc caaaatcccg cacgacaaat agcaataaac agcggtttat ctcatgaaat 8880 tcccgcaatg acagttaatg aggtctgcgg atcaggaatg aaggccgtta ttttggcgaa 8940 acaattgatt caattaggag aagcggaagt tttaattgct ggcgggattg agaatatgtc 9000 ccaagcacct aaattacaac gatttaatta cgaaacagaa agctatgatg cgcctttttc 9060 tagtatgatg tacgatgggt t 9081 <210> 81 <211> 8230 <212> DNA <213> Artificial Sequence <220> <223> pSNA(-E)free <400> 81 tttttttaag gcagttattg gtgcccttaa acgcctggtg ctacgcctga ataagtgata 60 ataagcggat gaatggcaga aattcgaaag caaattcgac ccggtcgtcg gttcagggca 120 gggtcgttaa atagccgctt atgtctattg ctggtttacc ggtttattga ctaccggaag 180 cagtgtgacc gtgtgcttct caaatgcctg aggccagttt gctcaggctc tccccgtgga 240 ggtaataatt gacgatatga tcatttattc tgcctcccag agcctgataa aaacggttag 300 cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag 360 atccggaaca taatggtgca gggcgcttgt ttcggcgtgg gtatggtggc aggccccgtg 420 gccgggggac tgttgggcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca 480 gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct accggacagc 540 ggtgcggact gttgtaactc agaataagaa atgaggccgc tcatggcgtt ccaatacgca 600 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg 660 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac 720 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac 780 aatttcacac aggaaacagc tatgaccatg attacgaatt cgagctcggt acccggggat 840 cctacctgac gctttttatc gcaactctct actgtttctc catacccgtt tttttgggct 900 agcaggagga attcaccatg gtaccaatga caaaaaaagt tggtgtcggt caggcacata 960 gtaagataat tttaataggg gaacatgcgg tcgtttacgg ttatcctgcc atttccctgc 1020 ctcttttgga ggtggaggtg acctgtaagg tagttcctgc agagagtcct tggcgccttt 1080 atgaggagga taccttgtcc atggcggttt atgcctcact ggagtatttg aatatcacag 1140 aagcctgcat tcgttgtgag attgactcgg ctatccctga gaaacggggg atgggttcgt 1200 cagcggctat cagcatagcg gccattcgtg cagtatttga ctactatcag gctgatctgc 1260 ctcatgatgt actagaaatc ttggtcaatc gagctgaaat gattgcccat atgaatccta 1320 gtggtttgga tgctaagacc tgtcttagtg accaacctat tcgctttatc aagaacgtag 1380 gatttacaga acttgagatg gatttatccg cctatttggt gattgccgat acgggtgttt 1440 atggtcatac tcgtgaagcc atccaagtgg ttcaaaataa gggcaaggat gccctaccgt 1500 ttttgcatgc cttgggagaa ttaacccagc aagcagaagt tgcgatttca caaaaagatg 1560 ctgaaggact gggacaaatc ctcagtcaag cgcatttaca tttaaaagaa attggagtca 1620 gtagccctga ggcagacttt ttggttgaaa cgactcttag ccatggtgct ctgggtgcca 1680 agatgagcgg tggtgggcta ggaggttgta tcatagcctt ggtaaccaat ttgacacacg 1740 cacaagaact agcagaaaga ttagaagaga aaggagctgt tcagacatgg atagagagcc 1800 tgtaacagta cgttcctacg caaatattgc tattatcaaa tattggggaa agaaaaaaga 1860 aaaagagatg gtgcctgcta ctagcagtat ttctctaact ttggaaaata tgtatacaga 1920 gacgaccttg tcgcctttac cagccaatgt aacagctgac gaattttaca tcaatggtca 1980 gctacaaaat gaggtcgagc atgccaagat gagtaagatt attgaccgtt atcgtccagc 2040 tggtgagggc tttgtccgta tcgatactca aaacaatatg cctacggcag cgggcctgtc 2100 ctcaagttct agtggtttgt ccgccctggt caaggcttgt aatgcttatt tcaagcttgg 2160 attggataga agtcagttgg cacaggaagc caaatttgcc tcaggctctt cttctcggag 2220 tttttatgga ccactaggag cctgggataa ggatagtgga gaaatttacc ctgtagagac 2280 agacttgaaa ctagctatga ttatgttggt gctagaggac aagaaaaaac caatctctag 2340 ccgtgacggg atgaaacttt gtgtggaaac ctcgacgact tttgacgact gggttcgtca 2400 gtctgagaag gactatcagg atatgctgat ttatctcaag gaaaatgatt ttgccaagat 2460 tggagaatta acggagaaaa atgctctggc tatgcatgct acgacaaaga ctgctagtcc 2520 agccttttct tatctgacgg atgcctctta tgaggctatg gcctttgttc gccagcttcg 2580 tgagaaagga gaggcctgct actttaccat ggatgctggt cccaatgtta aggtcttctg 2640 tcaggagaaa gacttggagc atttgtcaga aattttcggt cagcgttatc gcttgattgt 2700 gtcaaaaaca aaggatttga gtcaagatga ttgctgttaa aacttgcgga aaactctatt 2760 gggcaggtga atatgctatt ttagagccag ggcagttagc tttgataaag gatattccca 2820 tctatatgag ggctgagatt gctttttctg acagctaccg tatctattca gatatgtttg 2880 atttcgcagt ggacttaagg cccaatcctg actacagctt gattcaagaa acgattgctt 2940 tgatgggaga cttcctcgct gttcgcggtc agaatttaag acctttttcc ctaaaaatct 3000 gtggcaaaat ggaacgagaa gggaaaaagt ttggtctagg ttctagtggc agcgtcgttg 3060 tcttggttgt caaggcttta ctggctctct ataatctttc ggttgatcag aatctcttgt 3120 tcaagctgac tagcgctgtc ttgctcaagc gaggagacaa tggttccatg ggcgaccttg 3180 cctgtattgt ggcagaggat ttggttcttt accagtcatt tgatcgccag aaggcggctg 3240 cttggttaga agaagaaaac ttggcgacag ttctggagcg tgattgggga ttttttatct 3300 cacaagtgaa accaacttta gaatgtgatt tcttagtggg atggaccaag gaagtggctg 3360 tatcgagtca catggtccag caaatcaagc aaaatatcaa tcaaaatttt ttaagttcct 3420 caaaagaaac ggtggtttct ttggtcgaag ccttggagca ggggaaagcc gaaaaagtta 3480 tcgagcaagt agaagtagcc agcaagcttt tagaaggctt gagtacagat atttacacgc 3540 ctttgcttag acagttgaaa gaagccagtc aagatttgca ggccgttgcc aagagtagtg 3600 gtgctggtgg tggtgactgt ggcatcgccc tgagttttga tgcgcaatct tctcgaaaca 3660 ctttaaaaaa tcgttgggcc gatctgggga ttgagctctt atatcaagaa aggataggac 3720 atgacgacaa atcgtaatct agccccggga ggagagaaat tatgcaaacg gaacacgtca 3780 ttttattgaa tgcacaggga gttcccacgg gtacgctgga aaagtatgcc gcacacacgg 3840 cagacacccg cttacatctc gcgttctcca gttggctgtt taatgccaaa ggacaattat 3900 tagttacccg ccgcgcactg agcaaaaaag catggcctgg cgtgtggact aactcggttt 3960 gtgggcaccc acaactggga gaaagcaacg aagacgcagt gatccgccgt tgccgttatg 4020 agcttggcgt ggaaattacg cctcctgaat ctatctatcc tgactttcgc taccgcgcca 4080 ccgatccgag tggcattgtg gaaaatgaag tgtgtccggt atttgccgca cgcaccacta 4140 gtgcgttaca gatcaatgat gatgaagtga tggattatca atggtgtgat ttagcagatg 4200 tattacacgg tattgatgcc acgccgtggg cgttcagtcc gtggatggtg atgcaggcga 4260 caaatcgcga agccagaaaa cgattatctg catttaccca gcttaaataa gcatgcacta 4320 gagtcgagga aacagaccat ggagttgaaa acagtagtta ttattgatgc attacgaaca 4380 ccaattggaa aatataaagg cagcttaagt caagtaagtg ccgtagactt aggaacacat 4440 gttacaacac aacttttaaa aagacattcc actatttctg aagaaattga tcaagtaatc 4500 tttggaaatg ttttacaagc tggaaatggc caaaatcccg cacgacaaat agcaataaac 4560 agcggtttat ctcatgaaat tcccgcaatg acagttaatg aggtctgcgg atcaggaatg 4620 aaggccgtta ttttggcgaa acaattgatt caattaggag aagcggaagt tttaattgct 4680 ggcgggattg agaatatgtc ccaagcacct aaattacaac gatttaatta cgaaacagaa 4740 agctatgatg cgcctttttc tagtatgatg tacgatgggt taacgctatg ttggaaggtg 4800 tggccgagtt gttccgtgaa tggtttgcgg agcaaaagat tttattcagt attttaagta 4860 attatgccac ggagtcggtt gttacgatga aaacggctat tccagtttca cgtttaagta 4920 aggggagcaa tggccgggaa attgctgaaa aaattgtttt agcttcacgc tatgcttcat 4980 tagatcctta tcgggcagtc acgcataaca aagggatcat gaatggcatt gaagctgtcg 5040 ttttagctac aggaaatgat acacgcgctg ttagcgcttc ttgtcatgct tttgcggtga 5100 aggaaggtcg ctaccaaggt ttgactagtt ggacgctgga tggcgaacaa ctaattggtg 5160 aaatttcagt tccgcttgcg ttagccacgg ttggcggtgc cacaaaagtc ttacctaaat 5220 ctcaagcagc tgctgatttg ttagcagtga cggatgcaaa agaactaagt cgagtagtag 5280 cggctgttgg tttggcacaa aatttagcgg cgttacgggc cttagtctct gaaggaattc 5340 aaaaaggaca catggctcta caagcacgtt ctttagcgat gacggtcgga gctactggta 5400 aagaagttga ggcagtcgct caacaattaa aacgtcaaaa aacgatgaac caagaccgag 5460 ccttggctat tttaaatgat ttaagaaaac aataaaaaaa cagttcagca gaaattattc 5520 tgctgaactg ttttttttca cattaggtag ccgtttcagg ccacgagctc aggagttaaa 5580 gaaatgacaa ttgggattga taaaattagt ttttttgtgc ccccttatta tattgatatg 5640 acggcactgg ctgaagccag aaatgtagac cctggaaaat ttcatattgg tattgggcaa 5700 gaccaaatgg cggtgaaccc aatcagccaa gatattgtga catttgcagc caatgccgca 5760 gaagcgatct tgaccaaaga agataaagag gccattgata tggtgattgt cgggactgag 5820 tccagtatcg atgagtcaaa agcggccgca gttgtcttac atcgtttaat ggggattcaa 5880 cctttcgctc gctctttcga aatcaaggaa gcttgttacg gagcaacagc aggcttacag 5940 ttagctaaga atcacgtagc cttacatcca gataaaaaag tcttggttgt agcagcagat 6000 attgcaaaat atggattaaa ttctggcggt gagcctacac aaggagctgg ggcggttgca 6060 atgttagttg ctagtgaacc gcgcatcttg gctttaaaag aggataatgt gatgctgacg 6120 caagatatct atgacttttg gcgtccaaca ggccatccgt atcctatggt cgatggtcct 6180 ttgtcaaacg aaacctacat ccaatctttt gcccaagtct gggatgaaca taaaaaaaga 6240 accggtcttg attttgcaga ttatgatgct ttagcgttcc atattcctta cacaaaaatg 6300 ggcaaaaaag ccttattagc aaaaatctcc gaccaaactg aagcagaaca ggaacgaatt 6360 ttagcccgtt atgaagaaag catcatctat agtcgtcgcg taggaaactt gtatacgggt 6420 tcactttatc tgggactcat ttccctttta gaaaatgcaa cgactttaac cgcaggcaat 6480 caaattgggt tattcagtta tggttctggt gctgtcgctg aatttttcac tggtgaatta 6540 gtagctggtt atcaaaatca tttacaaaaa gaaactcatt tagcactgct agataatcgg 6600 acagaacttt ctatcgctga atatgaagcc atgtttgcag aaactttaga cacagatatt 6660 gatcaaacgt tagaagatga attaaaatat agtatttctg ctattaataa taccgttcgc 6720 tcttatcgaa actaactgca gcctcgacct gcaggcatgc aagcttggca ctggccgtcg 6780 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac 6840 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac 6900 agttgcgcag cctgaatggc gaatgagctt atcgatgata agctgtcaaa catgagaatt 6960 acaacttata tcgtatgggg ctgacttcag gtgctacatt tgaagagata aattgcactg 7020 aaatctagaa atattttatc tgattaataa gatgatcttc ttgagatcgt tttggtctgc 7080 gcgtaatctc ttgctctgaa aacgaaaaaa ccgccttgca gggcggtttt tcgaaggttc 7140 tctgagctac caactctttg aaccgaggta actggcttgg aggagcgcag tcaccaaaac 7200 ttgtcctttc agtttagcct taaccggcgc atgacttcaa gactaactcc tctaaatcaa 7260 ttaccagtgg ctgctgccag tggtgctttt gcatgtcttt ccgggttgga ctcaagacga 7320 tagttaccgg ataaggcgca gcggtcggac tgaacggggg gttcgtgcat acagtccagc 7380 ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg gaatgagaca aacgcggcca 7440 taacagcgga atgacaccgg taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc 7500 gccaggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacca ctgatttgag 7560 cgtcagattt cgtgatgctt gtcagggggg cggagcctat ggaaaaacgg ctttgccgcg 7620 gccctctcac ttccctgtta agtatcttcc tggcatcttc caggaaatct ccgccccgtt 7680 cgtaagccat ttccgctcgc cgcagtcgaa cgaccgagcg tagcgagtca gtgagcgagg 7740 aagcggaata tatcctgtat cacatattct gctgacgcac cggtgcagcc ttttttctcc 7800 tgccacatga agcacttcac tgacaccctc atcagtgcca acatagtaag ccagtataca 7860 ctccgctagc gctgatgtcc ggcggtgctt ttgccgttac gcaccacccc gtcagtagct 7920 gaacaggagg gacagctgat agaaacagaa gccactggag cacctcaaaa acaccatcat 7980 acactaaatc agtaagttgg cagcatcacc cgacgcactt tgcgccgaat aaatacctgt 8040 gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata ccgggaagcc 8100 ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt ccaactttca 8160 ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat tttcaggagc 8220 taaggaagct 8230 <210> 82 <211> 6678 <212> DNA <213> Artificial Sequence <220> <223> pTEFAmvaE <400> 82 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc 60 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc 120 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc 180 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga 240 taacaatttc acacaggaaa cagaccatgg ggctgcagga attcgatgcc atggagttga 300 aaacagtagt tattattgat gcattacgaa caccaattgg aaaatataaa ggcagcttaa 360 gtcaagtaag tgccgtagac ttaggaacac atgttacaac acaactttta aaaagacatt 420 ccactatttc tgaagaaatt gatcaagtaa tctttggaaa tgttttacaa gctggaaatg 480 gccaaaatcc cgcacgacaa atagcaataa acagcggttt gtctcatgaa attcccgcaa 540 tgacggttaa tgaggtctgc ggatcaggaa tgaaggccgt tattttggcg aaacaattga 600 ttcaattagg agaagcggaa gttttaattg ctggcgggat tgagaatatg tcccaagcac 660 ctaaattaca acgttttaat tacgaaacag aaagctacga tgcgcctttt tctagtatga 720 tgtatgatgg attaacggat gcctttagtg gtcaggcaat gggcttaact gctgaaaatg 780 tggccgaaaa gtatcatgta actagagaag agcaagatca attttctgta cattcacaat 840 taaaagcagc tcaagcacaa gcagaaggga tattcgctga cgaaatagcc ccattagaag 900 tatcaggaac gcttgtggag aaagatgaag ggattcgccc taattcgagc gttgagaagc 960 taggaacgct taaaacagtt tttaaagaag acggtactgt aacagcaggg aatgcatcaa 1020 ccattaatga tggggcttct gctttgatta ttgcttcaca agaatatgcc gaagcacacg 1080 gtcttcctta tttagctatt attcgagaca gtgtggaagt cggtattgat ccagcctata 1140 tgggaatttc gccgattaaa gccattcaaa aactgttagc gcgcaatcaa cttactacgg 1200 aagaaattga tctgtatgaa atcaacgaag catttgcagc aacttcaatc gtggtccaaa 1260 gagaactggc tttaccagag gaaaaggtca acatttatgg tggcggtatt tcattaggtc 1320 atgcgattgg tgccacaggt gctcgtttat taacgagttt aagttatcaa ttaaatcaaa 1380 aagaaaagaa atatggagtg gcttctttat gtatcggcgg tggcttagga ctcgctatgc 1440 tactagagag acctcagcaa aaaaaaaaca gccgatttta tcaaatgagt cctgaggaac 1500 gcctggcttc tcttcttaat gaaggccaga tttctgctga tacaaaaaaa gaatttgaaa 1560 atacggcttt atcttcgcag attgccaatc atatgattga aaatcaaatc agtgaaacag 1620 aagtgccgat gggcgttggc ttacatttaa cagtggacga aactgattat ttggtaccaa 1680 tggcgacaga agagccctca gttattgcgg ctttgagtaa tggtgcaaaa atagcacaag 1740 gatttaaaac agtgaatcaa caacgcttaa tgcgtggaca aatcgttttt tacgatgttg 1800 cagatcccga gtcattgatt gataaactac aagtaagaga agcggaagtt tttcaacaag 1860 cagagttaag ttatccatct atcgttaaac ggggcggcgg cttaagagat ttgcaatatc 1920 gtacttttga tgaatcattt gtatctgtcg actttttagt agatgttaag gatgcaatgg 1980 gggcaaatat cgttaacgct atgttggaag gtgtggccga gttgttccgt gaatggtttg 2040 cggagcaaaa gattttattc agtattttaa gtaattatgc cacggagtcg gttgttacga 2100 tgaaaacggc tattccagtt tcacgtttaa gtaaggggag caatggccgg gaaattgctg 2160 aaaaaattgt tttagcttca cgctatgctt cattagatcc ttatcgggca gtcacgcata 2220 acaaaggaat catgaatggc attgaagctg tagttttagc tacaggaaat gatacacgcg 2280 ctgttagcgc ttcttgtcat gcttttgcgg tgaaggaagg tcgctaccaa ggcttgacta 2340 gttggacgct ggatggcgaa caactaattg gtgaaatttc agttccgctt gctttagcca 2400 cggttggcgg tgccacaaaa gtcttaccta aatctcaagc agctgctgat ttgttagcag 2460 tgacggatgc aaaagaacta agtcgagtag tagcggctgt tggtttggca caaaatttag 2520 cggcgttacg ggccttagtc tctgaaggaa ttcaaaaagg acacatggct ctacaagcac 2580 gttctttagc gatgacggtc ggagctactg gtaaagaagt tgaggcagtc gctcaacaat 2640 taaaacgtca aaaaacgatg aaccaagacc gagccatggc tattttaaat gatttaagaa 2700 aacaataaaa aaacagttca gcagaaatta ttctgctgaa ctattttttt tcacattagg 2760 tagccgtttc aggccacgag ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat 2820 gcaagcttgg ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag 2880 aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac 2940 ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc 3000 cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac 3060 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg 3120 ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg 3180 ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg 3240 tttctacaaa ctctttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 3300 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 3360 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 3420 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 3480 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 3540 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg 3600 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 3660 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 3720 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 3780 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 3840 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct acagcaatgg 3900 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 3960 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 4020 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 4080 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 4140 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 4200 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 4260 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 4320 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 4380 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 4440 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 4500 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca 4560 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 4620 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 4680 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 4740 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 4800 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 4860 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 4920 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 4980 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 5040 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 5100 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc 5160 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta 5220 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg 5280 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct 5340 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag 5400 gttttcaccg tcatcaccga aacgcgcgag gcagcagatc aattcgcgcg cgaaggcgaa 5460 gcggcatgca tttacgttga caccatcgaa tggtgcaaaa cctttcgcgg tatggcatga 5520 tagcgcccgg aagagagtca attcagggtg gtgaatgtga aaccagtaac gttatacgat 5580 gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc 5640 cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt 5700 cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc 5760 tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat 5820 caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa 5880 gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg 5940 gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt 6000 gatgtctctg accagacacc catcaacagt attattttct cccatgaaga cggtacgcga 6060 ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca 6120 ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat 6180 caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa 6240 accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag 6300 atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc 6360 tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc 6420 atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct 6480 cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc 6540 accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 6600 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag 6660 ttagcgcgaa ttgatctg 6678 <210> 83 <211> 13288 <212> DNA <213> Artificial Sequence <220> <223> pT-DHBSRYbbO <400> 83 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc 60 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc 120 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc 180 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga 240 taacaatttc acacaggaaa cagaccatgg aattctacgt actagtgaat tcaggaggta 300 ataaatatgg gtctgatgct gattgattgg tgtgcactgg ctctggttgt tttcattggc 360 ctgccgcacg gcgcgctgga tgctgccatt tctttttcta tgatctcttc tgcaaaacgc 420 attgctcgtc tggctggtat tctgctgatc tatctgctgc tggcgaccgc gttcttcctg 480 atctggtatc agctgccagc gtttagcctg ctgatcttcc tgctgatctc cattatccac 540 tttggtatgg cagacttcaa cgcgtcccca agcaaactga aatggccgca tatcatcgcc 600 cacggcggtg ttgttactgt ttggctgccg ctgatccaga aaaacgaagt aactaaactg 660 tttagcatcc tgactaacgg tccgactccg atcctgtggg acatcctgct gattttcttc 720 ctgtgttggt ctattggcgt gtgtctgcac acgtacgaaa ccctgcgctc taaacattac 780 aacatcgcct ttgaactgat cggtctgatt ttcctggcgt ggtatgcgcc gcctctggtt 840 acgtttgcca cttacttctg cttcattcat tcccgtcgcc acttctcctt tgtgtggaag 900 cagctgcaac acatgtcttc caaaaagatg atgattggca gcgcgattat cctgtcctgt 960 acctcttggc tgatcggcgg tggtatctat ttcttcctga actccaaaat gatcgcctct 1020 gaggctgcgc tgcagactgt gttcatcggt ctggcggcac tgaccgtgcc gcacatgatt 1080 ctgatcgact tcatcttccg tccgcactct tcccgtatca aaatcaaaaa ctaattaatt 1140 aaaggaggta ataatatgac tcataaagca acggagatcc tgacaggtaa agttatgcaa 1200 aaatcggtct taattaccgg atgttccagt ggaattggcc tggaaagcgc gctcgaatta 1260 aaacgccagg gttttcatgt gctggcaggt tgccggaaac cggatgatgt tgagcgcatg 1320 aacagcatgg gatttaccgg cgtgttgatc gatctggatt caccagaaag tgttgatcgc 1380 gcagccgacg aggtgatcgc cctgaccgat aattgtctgt atgggatctt taacaatgcc 1440 ggattcggca tgtatggccc cctttccacc atcagccgtg cgcagatgga acagcagttt 1500 tccgccaact ttttcggcgc acaccagctc accatgcgcc tgttacccgc gatgttaccg 1560 cacggtgaag ggcgtattgt gatgacatca tcggtgatgg gattaatctc cacgccgggt 1620 cgtggcgctt acgcggccag taaatatgcg ctggaggcgt ggtcagatgc actgcgcatg 1680 gagctgcgcc acagcggaat taaagtcagc ctgatcgaac ccggtcccat tcgtactcgc 1740 ttcaccgaca acgtcaacca gacgcaaagt gataaaccag tcgaaaatcc cggcatcgcc 1800 gcccgcttta cgttgggacc ggaagcggtg gtggacaaag tacgccatgc ttttattagc 1860 gagaagccga agatgcgcta tccggtgacg ctggtgacct gggcggtaat ggtgcttaag 1920 cgcctgctgc cggggcgcgt gatggacaaa atattgcagg ggtaactagt aggaggtaat 1980 aaatatggtg agtggcagta aagcgggcgt ttcgcctcat cgcgaaatag aagtaatgag 2040 acaatccatt gacgatcacc tggctggcct gttacctgaa accgacagcc aggatatcgt 2100 cagccttgcg atgcgtgaag gcgtcatggc acccggtaaa cggatccgtc cgctgctgat 2160 gctgctggcc gcccgcgacc tccgctacca gggcagtatg cctacgctgc tcgatctcgc 2220 ctgcgccgtt gaactgaccc ataccgcgtc gctgatgctc gacgacatgc cctgcatgga 2280 caacgccgag ctgcgccgcg gtcagcccac tacccacaaa aaatttggtg agagcgtggc 2340 gatccttgcc tccgttgggc tgctctctaa agcctttggt ctgatcgccg ccaccggcga 2400 tctgccgggg gagaggcgtg cccaggcggt caacgagctc tctaccgccg tgggcgtgca 2460 gggcctggta ctggggcagt ttcgcgatct taacgatgcc gccctcgacc gtacccctga 2520 cgctatcctc agcaccaacc acctcaagac cggcattctg ttcagcgcga tgctgcagat 2580 cgtcgccatt gcttccgcct cgtcgccgag cacgcgagag acgctgcacg ccttcgccct 2640 cgacttcggc caggcgtttc aactgctgga cgatctgcgt gacgatcacc cggaaaccgg 2700 taaagatcgc aataaggacg cgggaaaatc gacgctggtc aaccggctgg gcgcagacgc 2760 ggcccggcaa aagctgcgcg agcatattga ttccgccgac aaacacctca cttttgcctg 2820 tccgcagggc ggcgccatcc gacagtttat gcatctgtgg tttggccatc accttgccga 2880 ctggtcaccg gtcatgaaaa tcgcctgata actcgaggag gtataaagga tgaaaaaaac 2940 cgttgtgatt ggcgcaggct ttggtggcct ggcgctggcg attcgcctgc aggcggcagg 3000 gatcccaacc gtactgctgg agcagcggga caagcccggc ggtcgggcct acgtctggca 3060 tgaccagggc tttacctttg acgccgggcc gacggtgatc accgatccta ccgcgcttga 3120 ggcgctgttc accctggccg gcaggcgcat ggaggattac gtcaggctgc tgccggtaaa 3180 acccttctac cgactctgct gggagtccgg gaagaccctc gactatgcta acgacagcgc 3240 cgagcttgag gcgcagatta cccagttcaa cccccgcgac gtcgagggct accggcgctt 3300 tctggcttac tcccaggcgg tattccagga gggatatttg cgcctcggca gcgtgccgtt 3360 cctctctttt cgcgacatgc tgcgcgccgg gccgcagctg cttaagctcc aggcgtggca 3420 gagcgtctac cagtcggttt cgcgctttat tgaggatgag catctgcggc aggccttctc 3480 gttccactcc ctgctggtag gcggcaaccc cttcaccacc tcgtccatct acaccctgat 3540 ccacgccctt gagcgggagt ggggggtctg gttccctgag ggcggcaccg gggcgctggt 3600 gaacggcatg gtgaagctgt ttaccgatct gggcggggag atcgaactca acgcccgggt 3660 cgaagagctg gtggtggccg ataaccgcgt aagccaggtc cggctggcgg atggtcggat 3720 ctttgacacc gacgccgtag cctcgaacgc tgacgtggtg aacacctata aaaagctgct 3780 cggccaccat ccggtggggc agaagcgggc ggcagcgctg gagcgcaaga gcatgagcaa 3840 ctcgctgttt gtgctctact tcggcctgaa ccagcctcat tcccagctgg cgcaccatac 3900 catctgtttt ggtccccgct accgggagct gatcgacgag atctttaccg gcagcgcgct 3960 ggcggatgac ttctcgctct acctgcactc gccctgcgtg accgatccct cgctcgcgcc 4020 tcccggctgc gccagcttct acgtgctggc cccggtgccg catcttggca acgcgccgct 4080 ggactgggcg caggaggggc cgaagctgcg cgaccgcatc tttgactacc ttgaagagcg 4140 ctatatgccc ggcctgcgta gccagctggt gacccagcgg atctttaccc cggcagactt 4200 ccacgacacg ctggatgcgc atctgggatc ggccttctcc atcgagccgc tgctgaccca 4260 aagcgcctgg ttccgcccgc acaaccgcga cagcgacatt gccaacctct acctggtggg 4320 cgcaggtact caccctgggg cgggcattcc tggcgtagtg gcctcggcga aagccaccgc 4380 cagcctgatg attgaggatc tgcaatgagc caaccgccgc tgcttgacca cgccacgcag 4440 accatggcca acggctcgaa aagttttgcc accgctgcga agctgttcga cccggccacc 4500 cgccgtagcg tgctgatgct ctacacctgg tgccgccact gcgatgacgt cattgacgac 4560 cagacccacg gcttcgccag cgaggccgcg gcggaggagg aggccaccca gcgcctggcc 4620 cggctgcgca cgctgaccct ggcggcgttt gaaggggccg agatgcagga tccggccttc 4680 gctgcctttc aggaggtggc gctgacccac ggtattacgc cccgcatggc gctcgatcac 4740 ctcgacggct ttgcgatgga cgtggctcag acccgctatg tcacctttga ggatacgctg 4800 cgctactgct atcacgtggc gggcgtggtg ggtctgatga tggccagggt gatgggcgtg 4860 cgggatgagc gggtgctgga tcgcgcctgc gatctggggc tggccttcca gctgacgaat 4920 atcgcccggg atattattga cgatgcggct attgaccgct gctatctgcc cgccgagtgg 4980 ctgcaggatg ccgggctgac cccggagaac tatgccgcgc gggagaatcg ggccgcgctg 5040 gcgcgggtgg cggagcggct tattgatgcc gcagagccgt actacatctc ctcccaggcc 5100 gggctacacg atctgccgcc gcgctgcgcc tgggcgatcg ccaccgcccg cagcgtctac 5160 cgggagatcg gtattaaggt aaaagcggcg ggaggcagcg cctgggatcg ccgccagcac 5220 accagcaaag gtgaaaaaat tgccatgctg atggcggcac cggggcaggt tattcgggcg 5280 aagacgacga gggtgacgcc gcgtccggcc ggtctttggc agcgtcccgt ttagtaatct 5340 agaggaggta ataaaatatg cttcgttcgt tgctcagagg cctcacgcat atcccccgcg 5400 tgaactccgc ccagcagccc agctgtgcac acgcgcgact ccagtttaag ctcaggagca 5460 tgcagatgac gctcatgcag cccagcatct cagccaatct gtcgcgcgcc gaggaccgca 5520 cagaccacat gaggggtgca agcacctggg caggcgggca gtcgcaggat gagctgatgc 5580 tgaaggacga gtgcatcttg gtggatgttg aggacaacat cacaggccat gccagcaagc 5640 tggagtgtca caagttccta ccacatcagc ctgcaggcct gctgcaccgg gccttctctg 5700 tgttcctgtt tgacgatcag gggcgactgc tgctgcaaca gcgtgcacgc tcaaaaatca 5760 ccttcccaag tgtgtggacg aacacctgct gcagccaccc tttacatggg cagaccccag 5820 atgaggtgga ccaactaagc caggtggccg acggaacagt acctggcgca aaggctgctg 5880 ccatccgcaa gttggagcac gagctgggga taccagcgca ccagctgccg gcaagcgcgt 5940 ttcgcttcct cacgcgtttg cactactgtg ccgcggacgt gcagccagct gcgacacaat 6000 cagcgctctg gggcgagcac gaaatggact acatcttgtt catccgggcc aacgtcacct 6060 tggcgcccaa ccctgacgag gtggacgaag tcaggtacgt gacgcaagag gagctgcggc 6120 agatgatgca gccggacaac gggctgcaat ggtcgccgtg gtttcgcatc atcgccgcgc 6180 gcttccttga gcgttggtgg gctgacctgg acgcggccct aaacactgac aaacacgagg 6240 attggggaac ggtgcatcac atcaacgaag cgtgataagc ggccgcgtcg acaggaggag 6300 cggctatgca accgcattat gatctgattc tcgtgggggc tggactcgcg aatggcctta 6360 tcgccctgcg tcttcagcag cagcaacctg atatgcgtat tttgcttatc gacgccgcac 6420 cccaggcggg cgggaatcat acgtggtcat ttcaccacga tgatttgact gagagccaac 6480 atcgttggat agctccgctg gtggttcatc actggcccga ctatcaggta cgctttccca 6540 cacgccgtcg taagctgaac agcggctact tttgtattac ttctcagcgt ttcgctgagg 6600 ttttacagcg acagtttggc ccgcacttgt ggatggatac cgcggtcgca gaggttaatg 6660 cggaatctgt tcggttgaaa aagggtcagg ttatcggtgc ccgcgcggtg attgacgggc 6720 ggggttatgc ggcaaattca gcactgagcg tgggcttcca ggcgtttatt ggccaggaat 6780 ggcgattgag ccacccgcat ggtttatcgt ctcccattat catggatgcc acggtcgatc 6840 agcaaaatgg ttatcgcttc gtgtacagcc tgccgctctc gccgaccaga ttgttaattg 6900 aagacacgca ctatattgat aatgcgacat tagatcctga atgcgcgcgg caaaatattt 6960 gcgactatgc cgcgcaacag ggttggcagc ttcagacact gctgcgagaa gaacagggcg 7020 ccttacccat tactctgtcg ggcaatgccg acgcattctg gcagcagcgc cccctggcct 7080 gtagtggatt acgtgccggt ctgttccatc ctaccaccgg ctattcactg ccgctggcgg 7140 ttgccgtggc cgaccgcctg agtgcacttg atgtctttac gtcggcctca attcaccatg 7200 ccattacgca ttttgcccgc gagcgctggc agcagcaggg ctttttccgc atgctgaatc 7260 gcatgctgtt tttagccgga cccgccgatt cacgctggcg ggttatgcag cgtttttatg 7320 gtttacctga agatttaatt gcccgttttt atgcgggaaa actcacgctg accgatcggc 7380 tacgtattct gagcggcaag ccgcctgttc cggtattagc agcattgcaa gccattatga 7440 cgactcatcg ttaactgcag gcatgcaagc tggggatcct acctgacgct ttttatcgca 7500 actctctact gtttctccat acccgttttt ttgggctagc aggaggaatt caccatggaa 7560 ttcatgagtt ttgatattgc caaatacccg accctggcac tggtcgactc cacccaggag 7620 ttacgactgt tgccgaaaga gagtttaccg aaactctgcg acgaactgcg ccgctattta 7680 ctcgacagcg tgagccgttc cagcgggcac ttcgcctccg ggctgggcac ggtcgaactg 7740 accgtggcgc tgcactatgt ctacaacacc ccgtttgacc aattgatttg ggatgtgggg 7800 catcaggctt atccgcataa aattttgacc ggacgccgcg acaaaatcgg caccatccgt 7860 cagaaaggcg gtctgcaccc gttcccgtgg cgcggcgaaa gcgaatatga cgtattaagc 7920 gtcgggcatt catcaacctc catcagtgcc ggaattggta ttgcggttgc tgccgaaaaa 7980 gaaggcaaaa atcgccgcac cgtctgtgtc attggcgatg gcgcgattac cgcaggcatg 8040 gcgtttgaag cgatgaatca cgcgggcgat atccgtcctg atatgctggt gattctcaac 8100 gacaatgaaa tgtcgatttc cgaaaatgtc ggcgcgctca acaaccatct ggcacagctg 8160 ctttccggta agctttactc ttcactgcgc gaaggcggga aaaaagtttt ctctggcgtg 8220 ccgccaatta aagagctgct caaacgcacc gaagaacata ttaaaggcat ggtagtgcct 8280 ggcacgttgt ttgaagagct gggctttaac tacatcggcc cggtggacgg tcacgatgtg 8340 ctggggctta tcaccacgct aaagaacatg cgcgacctga aaggcccgca gttcctgcat 8400 atcatgacca aaaaaggtcg tggttatgaa ccggcagaaa aagacccgat cactttccac 8460 gccgtgccta aatttgatcc ctccagcggt tgtttgccga aaagtagcgg cggtttgccg 8520 agctattcaa aaatctttgg cgactggttg tgcgaaacgg cagcgaaaga caacaagctg 8580 atggcgatta ctccggcgat gcgtgaaggt tccggcatgg tcgagttttc acgtaaattc 8640 ccggatcgct acttcgacgt ggcaattgcc gagcaacacg cggtgacctt tgctgcgggt 8700 ctggcgattg gtgggtacaa acccattgtc gcgatttact ccactttcct gcaacgcgcc 8760 tatgatcagg tgctgcatga cgtggcgatt caaaagcttc cggtcctgtt cgccatcgac 8820 cgcgcgggca ttgttggtgc tgacggtcaa acccatcagg gtgcttttga tctctcttac 8880 ctgcgctgca taccggaaat ggtcattatg accccgagcg atgaaaacga atgtcgccag 8940 atgctctata ccggctatca ctataacgat ggcccgtcag cggtgcgcta cccgcgtggc 9000 aacgcggtcg gcgtggaact gacgccgctg gaaaaactac caattggcaa aggcattgtg 9060 aagcgtcgtg gcgagaaact ggcgatcctt aactttggta cgctgatgcc agaagcggcg 9120 aaagtcgccg aatcgctgaa cgccacgctg gtcgatatgc gttttgtgaa accgcttgat 9180 gaagcgttaa ttctggaaat ggccgccagc catgaagcgc tggtcaccgt agaagaaaac 9240 gccattatgg gcggcgcagg cagcggcgtg aacgaagtgc tgatggccca tcgtaaacca 9300 gtacccgtgc tgaacattgg cctgccggac ttctttattc cgcaaggaac tcaggaagaa 9360 atgcgcgccg aactcggcct cgatgccgct ggtatggaag ccaaaatcaa ggcctggctg 9420 gcataaggta cccagcttgg ctgttttggc ggatgagaga agattttcag cctgatacag 9480 attaaatcag aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg 9540 gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt 9600 gtggggtctc cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca 9660 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag 9720 gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc 9780 aggacgcccg ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg 9840 cctttttgcg tttctacaaa ctctttttgt ttatttttct aaatacattc aaatatgtat 9900 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg 9960 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt 10020 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga 10080 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa 10140 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt 10200 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt 10260 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc 10320 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga 10380 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat 10440 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct 10500 acagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc 10560 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg 10620 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc 10680 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg 10740 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca 10800 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta 10860 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 10920 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 10980 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 11040 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 11100 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc 11160 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 11220 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 11280 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 11340 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 11400 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 11460 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 11520 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 11580 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 11640 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat 11700 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag 11760 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc 11820 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc 11880 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac 11940 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca 12000 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagcagatc aattcgcgcg 12060 cgaaggcgaa gcggcatgca tttacgttga caccatcgaa tggtgcaaaa cctttcgcgg 12120 tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatgtga aaccagtaac 12180 gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa 12240 ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct 12300 gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg 12360 cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc 12420 tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga 12480 agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa 12540 ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc 12600 gttatttctt gatgtctctg accagacacc catcaacagt attattttct cccatgaaga 12660 cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt 12720 agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct 12780 cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg 12840 ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc 12900 caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg 12960 tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc 13020 gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct 13080 gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa 13140 aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 13200 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 13260 ttaatgtgag ttagcgcgaa ttgatctg 13288 <210> 84 <211> 11378 <212> DNA <213> Artificial Sequence <220> <223> pT-HBSRYbbO <400> 84 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc 60 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc 120 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc 180 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga 240 taacaatttc acacaggaaa cagaccatgg aattctacgt actagtgaat tcaggaggta 300 ataaatatgg gtctgatgct gattgattgg tgtgcactgg ctctggttgt tttcattggc 360 ctgccgcacg gcgcgctgga tgctgccatt tctttttcta tgatctcttc tgcaaaacgc 420 attgctcgtc tggctggtat tctgctgatc tatctgctgc tggcgaccgc gttcttcctg 480 atctggtatc agctgccagc gtttagcctg ctgatcttcc tgctgatctc cattatccac 540 tttggtatgg cagacttcaa cgcgtcccca agcaaactga aatggccgca tatcatcgcc 600 cacggcggtg ttgttactgt ttggctgccg ctgatccaga aaaacgaagt aactaaactg 660 tttagcatcc tgactaacgg tccgactccg atcctgtggg acatcctgct gattttcttc 720 ctgtgttggt ctattggcgt gtgtctgcac acgtacgaaa ccctgcgctc taaacattac 780 aacatcgcct ttgaactgat cggtctgatt ttcctggcgt ggtatgcgcc gcctctggtt 840 acgtttgcca cttacttctg cttcattcat tcccgtcgcc acttctcctt tgtgtggaag 900 cagctgcaac acatgtcttc caaaaagatg atgattggca gcgcgattat cctgtcctgt 960 acctcttggc tgatcggcgg tggtatctat ttcttcctga actccaaaat gatcgcctct 1020 gaggctgcgc tgcagactgt gttcatcggt ctggcggcac tgaccgtgcc gcacatgatt 1080 ctgatcgact tcatcttccg tccgcactct tcccgtatca aaatcaaaaa ctaattaatt 1140 aaaggaggta ataatatgac tcataaagca acggagatcc tgacaggtaa agttatgcaa 1200 aaatcggtct taattaccgg atgttccagt ggaattggcc tggaaagcgc gctcgaatta 1260 aaacgccagg gttttcatgt gctggcaggt tgccggaaac cggatgatgt tgagcgcatg 1320 aacagcatgg gatttaccgg cgtgttgatc gatctggatt caccagaaag tgttgatcgc 1380 gcagccgacg aggtgatcgc cctgaccgat aattgtctgt atgggatctt taacaatgcc 1440 ggattcggca tgtatggccc cctttccacc atcagccgtg cgcagatgga acagcagttt 1500 tccgccaact ttttcggcgc acaccagctc accatgcgcc tgttacccgc gatgttaccg 1560 cacggtgaag ggcgtattgt gatgacatca tcggtgatgg gattaatctc cacgccgggt 1620 cgtggcgctt acgcggccag taaatatgcg ctggaggcgt ggtcagatgc actgcgcatg 1680 gagctgcgcc acagcggaat taaagtcagc ctgatcgaac ccggtcccat tcgtactcgc 1740 ttcaccgaca acgtcaacca gacgcaaagt gataaaccag tcgaaaatcc cggcatcgcc 1800 gcccgcttta cgttgggacc ggaagcggtg gtggacaaag tacgccatgc ttttattagc 1860 gagaagccga agatgcgcta tccggtgacg ctggtgacct gggcggtaat ggtgcttaag 1920 cgcctgctgc cggggcgcgt gatggacaaa atattgcagg ggtaactagt aggaggtaat 1980 aaatatggtg agtggcagta aagcgggcgt ttcgcctcat cgcgaaatag aagtaatgag 2040 acaatccatt gacgatcacc tggctggcct gttacctgaa accgacagcc aggatatcgt 2100 cagccttgcg atgcgtgaag gcgtcatggc acccggtaaa cggatccgtc cgctgctgat 2160 gctgctggcc gcccgcgacc tccgctacca gggcagtatg cctacgctgc tcgatctcgc 2220 ctgcgccgtt gaactgaccc ataccgcgtc gctgatgctc gacgacatgc cctgcatgga 2280 caacgccgag ctgcgccgcg gtcagcccac tacccacaaa aaatttggtg agagcgtggc 2340 gatccttgcc tccgttgggc tgctctctaa agcctttggt ctgatcgccg ccaccggcga 2400 tctgccgggg gagaggcgtg cccaggcggt caacgagctc tctaccgccg tgggcgtgca 2460 gggcctggta ctggggcagt ttcgcgatct taacgatgcc gccctcgacc gtacccctga 2520 cgctatcctc agcaccaacc acctcaagac cggcattctg ttcagcgcga tgctgcagat 2580 cgtcgccatt gcttccgcct cgtcgccgag cacgcgagag acgctgcacg ccttcgccct 2640 cgacttcggc caggcgtttc aactgctgga cgatctgcgt gacgatcacc cggaaaccgg 2700 taaagatcgc aataaggacg cgggaaaatc gacgctggtc aaccggctgg gcgcagacgc 2760 ggcccggcaa aagctgcgcg agcatattga ttccgccgac aaacacctca cttttgcctg 2820 tccgcagggc ggcgccatcc gacagtttat gcatctgtgg tttggccatc accttgccga 2880 ctggtcaccg gtcatgaaaa tcgcctgata actcgaggag gtataaagga tgaaaaaaac 2940 cgttgtgatt ggcgcaggct ttggtggcct ggcgctggcg attcgcctgc aggcggcagg 3000 gatcccaacc gtactgctgg agcagcggga caagcccggc ggtcgggcct acgtctggca 3060 tgaccagggc tttacctttg acgccgggcc gacggtgatc accgatccta ccgcgcttga 3120 ggcgctgttc accctggccg gcaggcgcat ggaggattac gtcaggctgc tgccggtaaa 3180 acccttctac cgactctgct gggagtccgg gaagaccctc gactatgcta acgacagcgc 3240 cgagcttgag gcgcagatta cccagttcaa cccccgcgac gtcgagggct accggcgctt 3300 tctggcttac tcccaggcgg tattccagga gggatatttg cgcctcggca gcgtgccgtt 3360 cctctctttt cgcgacatgc tgcgcgccgg gccgcagctg cttaagctcc aggcgtggca 3420 gagcgtctac cagtcggttt cgcgctttat tgaggatgag catctgcggc aggccttctc 3480 gttccactcc ctgctggtag gcggcaaccc cttcaccacc tcgtccatct acaccctgat 3540 ccacgccctt gagcgggagt ggggggtctg gttccctgag ggcggcaccg gggcgctggt 3600 gaacggcatg gtgaagctgt ttaccgatct gggcggggag atcgaactca acgcccgggt 3660 cgaagagctg gtggtggccg ataaccgcgt aagccaggtc cggctggcgg atggtcggat 3720 ctttgacacc gacgccgtag cctcgaacgc tgacgtggtg aacacctata aaaagctgct 3780 cggccaccat ccggtggggc agaagcgggc ggcagcgctg gagcgcaaga gcatgagcaa 3840 ctcgctgttt gtgctctact tcggcctgaa ccagcctcat tcccagctgg cgcaccatac 3900 catctgtttt ggtccccgct accgggagct gatcgacgag atctttaccg gcagcgcgct 3960 ggcggatgac ttctcgctct acctgcactc gccctgcgtg accgatccct cgctcgcgcc 4020 tcccggctgc gccagcttct acgtgctggc cccggtgccg catcttggca acgcgccgct 4080 ggactgggcg caggaggggc cgaagctgcg cgaccgcatc tttgactacc ttgaagagcg 4140 ctatatgccc ggcctgcgta gccagctggt gacccagcgg atctttaccc cggcagactt 4200 ccacgacacg ctggatgcgc atctgggatc ggccttctcc atcgagccgc tgctgaccca 4260 aagcgcctgg ttccgcccgc acaaccgcga cagcgacatt gccaacctct acctggtggg 4320 cgcaggtact caccctgggg cgggcattcc tggcgtagtg gcctcggcga aagccaccgc 4380 cagcctgatg attgaggatc tgcaatgagc caaccgccgc tgcttgacca cgccacgcag 4440 accatggcca acggctcgaa aagttttgcc accgctgcga agctgttcga cccggccacc 4500 cgccgtagcg tgctgatgct ctacacctgg tgccgccact gcgatgacgt cattgacgac 4560 cagacccacg gcttcgccag cgaggccgcg gcggaggagg aggccaccca gcgcctggcc 4620 cggctgcgca cgctgaccct ggcggcgttt gaaggggccg agatgcagga tccggccttc 4680 gctgcctttc aggaggtggc gctgacccac ggtattacgc cccgcatggc gctcgatcac 4740 ctcgacggct ttgcgatgga cgtggctcag acccgctatg tcacctttga ggatacgctg 4800 cgctactgct atcacgtggc gggcgtggtg ggtctgatga tggccagggt gatgggcgtg 4860 cgggatgagc gggtgctgga tcgcgcctgc gatctggggc tggccttcca gctgacgaat 4920 atcgcccggg atattattga cgatgcggct attgaccgct gctatctgcc cgccgagtgg 4980 ctgcaggatg ccgggctgac cccggagaac tatgccgcgc gggagaatcg ggccgcgctg 5040 gcgcgggtgg cggagcggct tattgatgcc gcagagccgt actacatctc ctcccaggcc 5100 gggctacacg atctgccgcc gcgctgcgcc tgggcgatcg ccaccgcccg cagcgtctac 5160 cgggagatcg gtattaaggt aaaagcggcg ggaggcagcg cctgggatcg ccgccagcac 5220 accagcaaag gtgaaaaaat tgccatgctg atggcggcac cggggcaggt tattcgggcg 5280 aagacgacga gggtgacgcc gcgtccggcc ggtctttggc agcgtcccgt ttagtaatct 5340 agaggaggta ataaaatatg cttcgttcgt tgctcagagg cctcacgcat atcccccgcg 5400 tgaactccgc ccagcagccc agctgtgcac acgcgcgact ccagtttaag ctcaggagca 5460 tgcagatgac gctcatgcag cccagcatct cagccaatct gtcgcgcgcc gaggaccgca 5520 cagaccacat gaggggtgca agcacctggg caggcgggca gtcgcaggat gagctgatgc 5580 tgaaggacga gtgcatcttg gtggatgttg aggacaacat cacaggccat gccagcaagc 5640 tggagtgtca caagttccta ccacatcagc ctgcaggcct gctgcaccgg gccttctctg 5700 tgttcctgtt tgacgatcag gggcgactgc tgctgcaaca gcgtgcacgc tcaaaaatca 5760 ccttcccaag tgtgtggacg aacacctgct gcagccaccc tttacatggg cagaccccag 5820 atgaggtgga ccaactaagc caggtggccg acggaacagt acctggcgca aaggctgctg 5880 ccatccgcaa gttggagcac gagctgggga taccagcgca ccagctgccg gcaagcgcgt 5940 ttcgcttcct cacgcgtttg cactactgtg ccgcggacgt gcagccagct gcgacacaat 6000 cagcgctctg gggcgagcac gaaatggact acatcttgtt catccgggcc aacgtcacct 6060 tggcgcccaa ccctgacgag gtggacgaag tcaggtacgt gacgcaagag gagctgcggc 6120 agatgatgca gccggacaac gggctgcaat ggtcgccgtg gtttcgcatc atcgccgcgc 6180 gcttccttga gcgttggtgg gctgacctgg acgcggccct aaacactgac aaacacgagg 6240 attggggaac ggtgcatcac atcaacgaag cgtgataagc ggccgcgtcg acaggaggag 6300 cggctatgca accgcattat gatctgattc tcgtgggggc tggactcgcg aatggcctta 6360 tcgccctgcg tcttcagcag cagcaacctg atatgcgtat tttgcttatc gacgccgcac 6420 cccaggcggg cgggaatcat acgtggtcat ttcaccacga tgatttgact gagagccaac 6480 atcgttggat agctccgctg gtggttcatc actggcccga ctatcaggta cgctttccca 6540 cacgccgtcg taagctgaac agcggctact tttgtattac ttctcagcgt ttcgctgagg 6600 ttttacagcg acagtttggc ccgcacttgt ggatggatac cgcggtcgca gaggttaatg 6660 cggaatctgt tcggttgaaa aagggtcagg ttatcggtgc ccgcgcggtg attgacgggc 6720 ggggttatgc ggcaaattca gcactgagcg tgggcttcca ggcgtttatt ggccaggaat 6780 ggcgattgag ccacccgcat ggtttatcgt ctcccattat catggatgcc acggtcgatc 6840 agcaaaatgg ttatcgcttc gtgtacagcc tgccgctctc gccgaccaga ttgttaattg 6900 aagacacgca ctatattgat aatgcgacat tagatcctga atgcgcgcgg caaaatattt 6960 gcgactatgc cgcgcaacag ggttggcagc ttcagacact gctgcgagaa gaacagggcg 7020 ccttacccat tactctgtcg ggcaatgccg acgcattctg gcagcagcgc cccctggcct 7080 gtagtggatt acgtgccggt ctgttccatc ctaccaccgg ctattcactg ccgctggcgg 7140 ttgccgtggc cgaccgcctg agtgcacttg atgtctttac gtcggcctca attcaccatg 7200 ccattacgca ttttgcccgc gagcgctggc agcagcaggg ctttttccgc atgctgaatc 7260 gcatgctgtt tttagccgga cccgccgatt cacgctggcg ggttatgcag cgtttttatg 7320 gtttacctga agatttaatt gcccgttttt atgcgggaaa actcacgctg accgatcggc 7380 tacgtattct gagcggcaag ccgcctgttc cggtattagc agcattgcaa gccattatga 7440 cgactcatcg ttaactgcag gcatgcaagc tggggatcct acctgacgct ttttatcgca 7500 actctctact gtttctccat acccgttttt ttgggctggc ggatgagaga agattttcag 7560 cctgatacag attaaatcag aacgcagaag cggtctgata aaacagaatt tgcctggcgg 7620 cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc 7680 cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat caaataaaac 7740 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc 7800 tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag 7860 ggtggcgggc aggacgcccg ccataaactg ccaggcatca aattaagcag aaggccatcc 7920 tgacggatgg cctttttgcg tttctacaaa ctctttttgt ttatttttct aaatacattc 7980 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag 8040 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg 8100 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt 8160 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt 8220 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt 8280 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa 8340 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag 8400 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac 8460 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac 8520 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac 8580 cacgatgcct acagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac 8640 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact 8700 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg 8760 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt 8820 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat 8880 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta 8940 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa 9000 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 9060 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 9120 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 9180 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc 9240 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 9300 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 9360 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 9420 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 9480 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 9540 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 9600 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 9660 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc 9720 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga 9780 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga 9840 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg 9900 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact 9960 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac 10020 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc 10080 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagcagatc 10140 aattcgcgcg cgaaggcgaa gcggcatgca tttacgttga caccatcgaa tggtgcaaaa 10200 cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatgtga 10260 aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc 10320 gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga 10380 tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt 10440 tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg 10500 cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa 10560 gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc 10620 tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta 10680 atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt attattttct 10740 cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa 10800 tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc 10860 ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg 10920 ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga 10980 tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc 11040 tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt 11100 atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg 11160 accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct 11220 cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt 11280 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag 11340 cgcaacgcaa ttaatgtgag ttagcgcgaa ttgatctg 11378 <210> 85 <211> 13938 <212> DNA <213> Artificial Sequence <220> <223> pT-HBSREYbbO <400> 85 tcgacaggag gagcggctat gcaaccgcat tatgatctga ttctcgtggg ggctggactc 60 gcgaatggcc ttatcgccct gcgtcttcag cagcagcaac ctgatatgcg tattttgctt 120 atcgacgccg caccccaggc gggcgggaat catacgtggt catttcacca cgatgatttg 180 actgagagcc aacatcgttg gatagctccg ctggtggttc atcactggcc cgactatcag 240 gtacgctttc ccacacgccg tcgtaagctg aacagcggct acttttgtat tacttctcag 300 cgtttcgctg aggttttaca gcgacagttt ggcccgcact tgtggatgga taccgcggtc 360 gcagaggtta atgcggaatc tgttcggttg aaaaagggtc aggttatcgg tgcccgcgcg 420 gtgattgacg ggcggggtta tgcggcaaat tcagcactga gcgtgggctt ccaggcgttt 480 attggccagg aatggcgatt gagccacccg catggtttat cgtctcccat tatcatggat 540 gccacggtcg atcagcaaaa tggttatcgc ttcgtgtaca gcctgccgct ctcgccgacc 600 agattgttaa ttgaagacac gcactatatt gataatgcga cattagatcc tgaatgcgcg 660 cggcaaaata tttgcgacta tgccgcgcaa cagggttggc agcttcagac actgctgcga 720 gaagaacagg gcgccttacc cattactctg tcgggcaatg ccgacgcatt ctggcagcag 780 cgccccctgg cctgtagtgg attacgtgcc ggtctgttcc atcctaccac cggctattca 840 ctgccgctgg cggttgccgt ggccgaccgc ctgagtgcac ttgatgtctt tacgtcggcc 900 tcaattcacc atgccattac gcattttgcc cgcgagcgct ggcagcagca gggctttttc 960 cgcatgctga atcgcatgct gtttttagcc ggacccgccg attcacgctg gcgggttatg 1020 cagcgttttt atggtttacc tgaagattta attgcccgtt tttatgcggg aaaactcacg 1080 ctgaccgatc ggctacgtat tctgagcggc aagccgcctg ttccggtatt agcagcattg 1140 caagccatta tgacgactca tcgttaactg caggcatgca agctggggat cctacctgac 1200 gctttttatc gcaactctct actgtttctc catacccgtt tttttgggct ggcggatgag 1260 agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga 1320 atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga 1380 aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg 1440 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 1500 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag 1560 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaattaag 1620 cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactctttt tgtttatttt 1680 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 1740 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 1800 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 1860 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 1920 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 1980 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac 2040 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 2100 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 2160 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 2220 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 2280 acgagcgtga caccacgatg cctacagcaa tggcaacaac gttgcgcaaa ctattaactg 2340 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 2400 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 2460 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 2520 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 2580 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 2640 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 2700 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 2760 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 2820 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 2880 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc 2940 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 3000 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 3060 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 3120 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 3180 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 3240 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 3300 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 3360 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 3420 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 3480 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 3540 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg 3600 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa 3660 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc 3720 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc 3780 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc 3840 gaggcagcag atcaattcgc gcgcgaaggc gaagcggcat gcatttacgt tgacaccatc 3900 gaatggtgca aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg 3960 gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat 4020 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa 4080 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg 4140 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg 4200 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg 4260 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa 4320 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa 4380 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac 4440 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg 4500 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt 4560 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa 4620 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc 4680 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt 4740 accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa 4800 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg 4860 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag 4920 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc 4980 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa 5040 agcgggcagt gagcgcaacg caattaatgt gagttagcgc gaattgatct ggtttgacag 5100 cttatcatcg actgcacggt gcaccaatgc ttctggcgtc aggcagccat cggaagctgt 5160 ggtatggctg tgcaggtcgt aaatcactgc ataattcgtg tcgctcaagg cgcactcccg 5220 ttctggataa tgttttttgc gccgacatca taacggttct ggcaaatatt ctgaaatgag 5280 ctgttgacaa ttaatcatcc ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt 5340 cacacaggaa acagaccatg gaattctacg tactagtgaa ttcaggaggt aataaatatg 5400 ggtctgatgc tgattgattg gtgtgcactg gctctggttg ttttcattgg cctgccgcac 5460 ggcgcgctgg atgctgccat ttctttttct atgatctctt ctgcaaaacg cattgctcgt 5520 ctggctggta ttctgctgat ctatctgctg ctggcgaccg cgttcttcct gatctggtat 5580 cagctgccag cgtttagcct gctgatcttc ctgctgatct ccattatcca ctttggtatg 5640 gcagacttca acgcgtcccc aagcaaactg aaatggccgc atatcatcgc ccacggcggt 5700 gttgttactg tttggctgcc gctgatccag aaaaacgaag taactaaact gtttagcatc 5760 ctgactaacg gtccgactcc gatcctgtgg gacatcctgc tgattttctt cctgtgttgg 5820 tctattggcg tgtgtctgca cacgtacgaa accctgcgct ctaaacatta caacatcgcc 5880 tttgaactga tcggtctgat tttcctggcg tggtatgcgc cgcctctggt tacgtttgcc 5940 acttacttct gcttcattca ttcccgtcgc cacttctcct ttgtgtggaa gcagctgcaa 6000 cacatgtctt ccaaaaagat gatgattggc agcgcgatta tcctgtcctg tacctcttgg 6060 ctgatcggcg gtggtatcta tttcttcctg aactccaaaa tgatcgcctc tgaggctgcg 6120 ctgcagactg tgttcatcgg tctggcggca ctgaccgtgc cgcacatgat tctgatcgac 6180 ttcatcttcc gtccgcactc ttcccgtatc aaaatcaaaa actaattaat taaaggaggt 6240 aataatatga ctcataaagc aacggagatc ctgacaggta aagttatgca aaaatcggtc 6300 ttaattaccg gatgttccag tggaattggc ctggaaagcg cgctcgaatt aaaacgccag 6360 ggttttcatg tgctggcagg ttgccggaaa ccggatgatg ttgagcgcat gaacagcatg 6420 ggatttaccg gcgtgttgat cgatctggat tcaccagaaa gtgttgatcg cgcagccgac 6480 gaggtgatcg ccctgaccga taattgtctg tatgggatct ttaacaatgc cggattcggc 6540 atgtatggcc ccctttccac catcagccgt gcgcagatgg aacagcagtt ttccgccaac 6600 tttttcggcg cacaccagct caccatgcgc ctgttacccg cgatgttacc gcacggtgaa 6660 gggcgtattg tgatgacatc atcggtgatg ggattaatct ccacgccggg tcgtggcgct 6720 tacgcggcca gtaaatatgc gctggaggcg tggtcagatg cactgcgcat ggagctgcgc 6780 cacagcggaa ttaaagtcag cctgatcgaa cccggtccca ttcgtactcg cttcaccgac 6840 aacgtcaacc agacgcaaag tgataaacca gtcgaaaatc ccggcatcgc cgcccgcttt 6900 acgttgggac cggaagcggt ggtggacaaa gtacgccatg cttttattag cgagaagccg 6960 aagatgcgct atccggtgac gctggtgacc tgggcggtaa tggtgcttaa gcgcctgctg 7020 ccggggcgcg tgatggacaa aatattgcag gggtaactag taggaggtaa taaatatggt 7080 gagtggcagt aaagcgggcg tttcgcctca tcgcgaaata gaagtaatga gacaatccat 7140 tgacgatcac ctggctggcc tgttacctga aaccgacagc caggatatcg tcagccttgc 7200 gatgcgtgaa ggcgtcatgg cacccggtaa acggatccgt ccgctgctga tgctgctggc 7260 cgcccgcgac ctccgctacc agggcagtat gcctacgctg ctcgatctcg cctgcgccgt 7320 tgaactgacc cataccgcgt cgctgatgct cgacgacatg ccctgcatgg acaacgccga 7380 gctgcgccgc ggtcagccca ctacccacaa aaaatttggt gagagcgtgg cgatccttgc 7440 ctccgttggg ctgctctcta aagcctttgg tctgatcgcc gccaccggcg atctgccggg 7500 ggagaggcgt gcccaggcgg tcaacgagct ctctaccgcc gtgggcgtgc agggcctggt 7560 actggggcag tttcgcgatc ttaacgatgc cgccctcgac cgtacccctg acgctatcct 7620 cagcaccaac cacctcaaga ccggcattct gttcagcgcg atgctgcaga tcgtcgccat 7680 tgcttccgcc tcgtcgccga gcacgcgaga gacgctgcac gccttcgccc tcgacttcgg 7740 ccaggcgttt caactgctgg acgatctgcg tgacgatcac ccggaaaccg gtaaagatcg 7800 caataaggac gcgggaaaat cgacgctggt caaccggctg ggcgcagacg cggcccggca 7860 aaagctgcgc gagcatattg attccgccga caaacacctc acttttgcct gtccgcaggg 7920 cggcgccatc cgacagttta tgcatctgtg gtttggccat caccttgccg actggtcacc 7980 ggtcatgaaa atcgcctgat aactcgagga ggtataaagg atgaaaaaaa ccgttgtgat 8040 tggcgcaggc tttggtggcc tggcgctggc gattcgcctg caggcggcag ggatcccaac 8100 cgtactgctg gagcagcggg acaagcccgg cggtcgggcc tacgtctggc atgaccaggg 8160 ctttaccttt gacgccgggc cgacggtgat caccgatcct accgcgcttg aggcgctgtt 8220 caccctggcc ggcaggcgca tggaggatta cgtcaggctg ctgccggtaa aacccttcta 8280 ccgactctgc tgggagtccg ggaagaccct cgactatgct aacgacagcg ccgagcttga 8340 ggcgcagatt acccagttca acccccgcga cgtcgagggc taccggcgct ttctggctta 8400 ctcccaggcg gtattccagg agggatattt gcgcctcggc agcgtgccgt tcctctcttt 8460 tcgcgacatg ctgcgcgccg ggccgcagct gcttaagctc caggcgtggc agagcgtcta 8520 ccagtcggtt tcgcgcttta ttgaggatga gcatctgcgg caggccttct cgttccactc 8580 cctgctggta ggcggcaacc ccttcaccac ctcgtccatc tacaccctga tccacgccct 8640 tgagcgggag tggggggtct ggttccctga gggcggcacc ggggcgctgg tgaacggcat 8700 ggtgaagctg tttaccgatc tgggcgggga gatcgaactc aacgcccggg tcgaagagct 8760 ggtggtggcc gataaccgcg taagccaggt ccggctggcg gatggtcgga tctttgacac 8820 cgacgccgta gcctcgaacg ctgacgtggt gaacacctat aaaaagctgc tcggccacca 8880 tccggtgggg cagaagcggg cggcagcgct ggagcgcaag agcatgagca actcgctgtt 8940 tgtgctctac ttcggcctga accagcctca ttcccagctg gcgcaccata ccatctgttt 9000 tggtccccgc taccgggagc tgatcgacga gatctttacc ggcagcgcgc tggcggatga 9060 cttctcgctc tacctgcact cgccctgcgt gaccgatccc tcgctcgcgc ctcccggctg 9120 cgccagcttc tacgtgctgg ccccggtgcc gcatcttggc aacgcgccgc tggactgggc 9180 gcaggagggg ccgaagctgc gcgaccgcat ctttgactac cttgaagagc gctatatgcc 9240 cggcctgcgt agccagctgg tgacccagcg gatctttacc ccggcagact tccacgacac 9300 gctggatgcg catctgggat cggccttctc catcgagccg ctgctgaccc aaagcgcctg 9360 gttccgcccg cacaaccgcg acagcgacat tgccaacctc tacctggtgg gcgcaggtac 9420 tcaccctggg gcgggcattc ctggcgtagt ggcctcggcg aaagccaccg ccagcctgat 9480 gattgaggat ctgcaatgag ccaaccgccg ctgcttgacc acgccacgca gaccatggcc 9540 aacggctcga aaagttttgc caccgctgcg aagctgttcg acccggccac ccgccgtagc 9600 gtgctgatgc tctacacctg gtgccgccac tgcgatgacg tcattgacga ccagacccac 9660 ggcttcgcca gcgaggccgc ggcggaggag gaggccaccc agcgcctggc ccggctgcgc 9720 acgctgaccc tggcggcgtt tgaaggggcc gagatgcagg atccggcctt cgctgccttt 9780 caggaggtgg cgctgaccca cggtattacg ccccgcatgg cgctcgatca cctcgacggc 9840 tttgcgatgg acgtggctca gacccgctat gtcacctttg aggatacgct gcgctactgc 9900 tatcacgtgg cgggcgtggt gggtctgatg atggccaggg tgatgggcgt gcgggatgag 9960 cgggtgctgg atcgcgcctg cgatctgggg ctggccttcc agctgacgaa tatcgcccgg 10020 gatattattg acgatgcggc tattgaccgc tgctatctgc ccgccgagtg gctgcaggat 10080 gccgggctga ccccggagaa ctatgccgcg cgggagaatc gggccgcgct ggcgcgggtg 10140 gcggagcggc ttattgatgc cgcagagccg tactacatct cctcccaggc cgggctacac 10200 gatctgccgc cgcgctgcgc ctgggcgatc gccaccgccc gcagcgtcta ccgggagatc 10260 ggtattaagg taaaagcggc gggaggcagc gcctgggatc gccgccagca caccagcaaa 10320 ggtgaaaaaa ttgccatgct gatggcggca ccggggcagg ttattcgggc gaagacgacg 10380 agggtgacgc cgcgtccggc cggtctttgg cagcgtcccg tttagtaatc tagaggaggt 10440 aataaaatat gcttcgttcg ttgctcagag gcctcacgca tatcccccgc gtgaactccg 10500 cccagcagcc cagctgtgca cacgcgcgac tccagtttaa gctcaggagc atgcagatga 10560 cgctcatgca gcccagcatc tcagccaatc tgtcgcgcgc cgaggaccgc acagaccaca 10620 tgaggggtgc aagcacctgg gcaggcgggc agtcgcagga tgagctgatg ctgaaggacg 10680 agtgcatctt ggtggatgtt gaggacaaca tcacaggcca tgccagcaag ctggagtgtc 10740 acaagttcct accacatcag cctgcaggcc tgctgcaccg ggccttctct gtgttcctgt 10800 ttgacgatca ggggcgactg ctgctgcaac agcgtgcacg ctcaaaaatc accttcccaa 10860 gtgtgtggac gaacacctgc tgcagccacc ctttacatgg gcagacccca gatgaggtgg 10920 accaactaag ccaggtggcc gacggaacag tacctggcgc aaaggctgct gccatccgca 10980 agttggagca cgagctgggg ataccagcgc accagctgcc ggcaagcgcg tttcgcttcc 11040 tcacgcgttt gcactactgt gccgcggacg tgcagccagc tgcgacacaa tcagcgctct 11100 ggggcgagca cgaaatggac tacatcttgt tcatccgggc caacgtcacc ttggcgccca 11160 accctgacga ggtggacgaa gtcaggtacg tgacgcaaga ggagctgcgg cagatgatgc 11220 agccggacaa cgggctgcaa tggtcgccgt ggtttcgcat catcgccgcg cgcttccttg 11280 agcgttggtg ggctgacctg gacgcggccc taaacactga caaacacgag gattggggaa 11340 cggtgcatca catcaacgaa gcgtgataag cggccgcgct gttgacaatt aatcatccgg 11400 ctcgtataat gtgtggaatt gtgagcggat aacaatttca cacaggaaac agaccatgga 11460 gttgaaaaca gtagttatta ttgatgcatt acgaacacca attggaaaat ataaaggcag 11520 cttaagtcaa gtaagtgccg tagacttagg aacacatgtt acaacacaac ttttaaaaag 11580 acattccact atttctgaag aaattgatca agtaatcttt ggaaatgttt tacaagctgg 11640 aaatggccaa aatcccgcac gacaaatagc aataaacagc ggtttatctc atgaaattcc 11700 cgcaatgaca gttaatgagg tctgcggatc aggaatgaag gccgttattt tggcgaaaca 11760 attgattcaa ttaggagaag cggaagtttt aattgctggc gggattgaga atatgtccca 11820 agcacctaaa ttacaacgat ttaattacga aacagaaagc tatgatgcgc ctttttctag 11880 tatgatgtac gatgggttaa cggatgcctt tagtggtcaa gcaatgggct taactgctga 11940 aaatgtggcc gaaaagtatc atgtaactag agaagagcaa gatcaatttt ctgtacattc 12000 acaattaaaa gcagctcaag cacaagcaga agggatattc gctgacgaaa tagccccatt 12060 agaagtatca ggaacgcttg tggagaaaga tgaagggatt cgccctaatt cgagcgttga 12120 gaagctagga acgcttaaaa cagtttttaa agaagacggt actgtaacag cagggaatgc 12180 atcaaccatt aatgatgggg cttctgcttt gattattgct tcacaagaat atgccgaagc 12240 acacggtctt ccttatttag ctattattcg agacagtgtg gaagtcggta ttgatccagc 12300 ctatatggga atttcgccga ttaaagccat tcaaaaactg ttagcgcgca atcaacttac 12360 tacggaagaa attgatctgt atgaaatcaa cgaagcattt gcagcaactt caatcgtggt 12420 ccaaagagaa ctggctttac cagaggaaaa ggtcaacatt tatggtggcg gtatttcatt 12480 aggtcatgcg attggtgcca caggtgctcg tttattaacg agtttaagtt atcaattaaa 12540 tcaaaaagaa aagaaatatg gagtggcttc tttatgtatc ggcggtggct taggactcgc 12600 tatgctacta gagagacctc agcaaaaaaa aaacagccga ttttatcaaa tgagtcctga 12660 ggaacgcctg gcttctcttc ttaatgaagg ccagatttct gctgatacaa aaaaagaatt 12720 tgaaaatacg gctttatctt cgcagattgc caatcatatg attgaaaatc aaatcagtga 12780 aacagaagtg ccgatgggcg ttggcttaca tttaacagtg gacgaaactg attatttggt 12840 accaatggcg acagaagagc cctcagtgat tgcggctttg agtaatggtg caaaaatagc 12900 acaaggattt aaaacagtga atcaacaacg tttaatgcgt ggacaaatcg ttttttacga 12960 tgttgcagac gccgagtcat tgattgatga actacaagta agagaaacgg aaatttttca 13020 acaagcagag ttaagttatc catctatcgt taaacgcggc ggcggcttaa gagatttgca 13080 atatcgtgct tttgatgaat catttgtatc tgtcgacttt ttagtagatg ttaaggatgc 13140 aatgggggca aatatcgtta acgctatgtt ggaaggtgtg gccgagttgt tccgtgaatg 13200 gtttgcggag caaaagattt tattcagtat tttaagtaat tatgccacgg agtcggttgt 13260 tacgatgaaa acggctattc cagtttcacg tttaagtaag gggagcaatg gccgggaaat 13320 tgctgaaaaa attgttttag cttcacgcta tgcttcatta gatccttatc gggcagtcac 13380 gcataacaaa gggatcatga atggcattga agctgtcgtt ttagctacag gaaatgatac 13440 acgcgctgtt agcgcttctt gtcatgcttt tgcggtgaag gaaggtcgct accaaggttt 13500 gactagttgg acgctggatg gcgaacaact aattggtgaa atttcagttc cgcttgcgtt 13560 agccacggtt ggcggtgcca caaaagtctt acctaaatct caagcagctg ctgatttgtt 13620 agcagtgacg gatgcaaaag aactaagtcg agtagtagcg gctgttggtt tggcacaaaa 13680 tttagcggcg ttacgggcct tagtctctga aggaattcaa aaaggacaca tggctctaca 13740 agcacgttct ttagcgatga cggtcggagc tactggtaaa gaagttgagg cagtcgctca 13800 acaattaaaa cgtcaaaaaa cgatgaacca agaccgagcc ttggctattt taaatgattt 13860 aagaaaacaa taaaaaaaca gttcagcaga aattattctg ctgaactgtt ttttttcaca 13920 ttaggtagcc gtttcagc 13938 <210> 86 <211> 12898 <212> DNA <213> Artificial Sequence <220> <223> pT-HBSREYbbOfree <400> 86 agcttgcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 60 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 120 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 180 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 240 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 300 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 360 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 420 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 480 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 540 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 600 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 660 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg 720 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc 780 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc 840 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg 900 catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg 960 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta 1020 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc 1080 gaaacgcgcg aggcagcaga tcaattcgcg cgcgaaggcg aagcggcatg catttacgtt 1140 gacaccatcg aatggtgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt 1200 caattcaggg tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt 1260 gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg 1320 cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa 1380 caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac 1440 gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg 1500 gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt 1560 ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt 1620 gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca 1680 cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg 1740 gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg 1800 cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg 1860 gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat 1920 gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg 1980 cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac 2040 gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc 2100 ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag 2160 ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg 2220 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc 2280 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagcgcg aattgatctg 2340 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc 2400 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc 2460 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc 2520 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga 2580 taacaatttc acacaggaaa cagaccatgg aattctacgt actagtgaat tcaggaggta 2640 ataaatatgg gtctgatgct gattgattgg tgtgcactgg ctctggttgt tttcattggc 2700 ctgccgcacg gcgcgctgga tgctgccatt tctttttcta tgatctcttc tgcaaaacgc 2760 attgctcgtc tggctggtat tctgctgatc tatctgctgc tggcgaccgc gttcttcctg 2820 atctggtatc agctgccagc gtttagcctg ctgatcttcc tgctgatctc cattatccac 2880 tttggtatgg cagacttcaa cgcgtcccca agcaaactga aatggccgca tatcatcgcc 2940 cacggcggtg ttgttactgt ttggctgccg ctgatccaga aaaacgaagt aactaaactg 3000 tttagcatcc tgactaacgg tccgactccg atcctgtggg acatcctgct gattttcttc 3060 ctgtgttggt ctattggcgt gtgtctgcac acgtacgaaa ccctgcgctc taaacattac 3120 aacatcgcct ttgaactgat cggtctgatt ttcctggcgt ggtatgcgcc gcctctggtt 3180 acgtttgcca cttacttctg cttcattcat tcccgtcgcc acttctcctt tgtgtggaag 3240 cagctgcaac acatgtcttc caaaaagatg atgattggca gcgcgattat cctgtcctgt 3300 acctcttggc tgatcggcgg tggtatctat ttcttcctga actccaaaat gatcgcctct 3360 gaggctgcgc tgcagactgt gttcatcggt ctggcggcac tgaccgtgcc gcacatgatt 3420 ctgatcgact tcatcttccg tccgcactct tcccgtatca aaatcaaaaa ctaattaatt 3480 aaaggaggta ataatatgac tcataaagca acggagatcc tgacaggtaa agttatgcaa 3540 aaatcggtct taattaccgg atgttccagt ggaattggcc tggaaagcgc gctcgaatta 3600 aaacgccagg gttttcatgt gctggcaggt tgccggaaac cggatgatgt tgagcgcatg 3660 aacagcatgg gatttaccgg cgtgttgatc gatctggatt caccagaaag tgttgatcgc 3720 gcagccgacg aggtgatcgc cctgaccgat aattgtctgt atgggatctt taacaatgcc 3780 ggattcggca tgtatggccc cctttccacc atcagccgtg cgcagatgga acagcagttt 3840 tccgccaact ttttcggcgc acaccagctc accatgcgcc tgttacccgc gatgttaccg 3900 cacggtgaag ggcgtattgt gatgacatca tcggtgatgg gattaatctc cacgccgggt 3960 cgtggcgctt acgcggccag taaatatgcg ctggaggcgt ggtcagatgc actgcgcatg 4020 gagctgcgcc acagcggaat taaagtcagc ctgatcgaac ccggtcccat tcgtactcgc 4080 ttcaccgaca acgtcaacca gacgcaaagt gataaaccag tcgaaaatcc cggcatcgcc 4140 gcccgcttta cgttgggacc ggaagcggtg gtggacaaag tacgccatgc ttttattagc 4200 gagaagccga agatgcgcta tccggtgacg ctggtgacct gggcggtaat ggtgcttaag 4260 cgcctgctgc cggggcgcgt gatggacaaa atattgcagg ggtaactagt aggaggtaat 4320 aaatatggtg agtggcagta aagcgggcgt ttcgcctcat cgcgaaatag aagtaatgag 4380 acaatccatt gacgatcacc tggctggcct gttacctgaa accgacagcc aggatatcgt 4440 cagccttgcg atgcgtgaag gcgtcatggc acccggtaaa cggatccgtc cgctgctgat 4500 gctgctggcc gcccgcgacc tccgctacca gggcagtatg cctacgctgc tcgatctcgc 4560 ctgcgccgtt gaactgaccc ataccgcgtc gctgatgctc gacgacatgc cctgcatgga 4620 caacgccgag ctgcgccgcg gtcagcccac tacccacaaa aaatttggtg agagcgtggc 4680 gatccttgcc tccgttgggc tgctctctaa agcctttggt ctgatcgccg ccaccggcga 4740 tctgccgggg gagaggcgtg cccaggcggt caacgagctc tctaccgccg tgggcgtgca 4800 gggcctggta ctggggcagt ttcgcgatct taacgatgcc gccctcgacc gtacccctga 4860 cgctatcctc agcaccaacc acctcaagac cggcattctg ttcagcgcga tgctgcagat 4920 cgtcgccatt gcttccgcct cgtcgccgag cacgcgagag acgctgcacg ccttcgccct 4980 cgacttcggc caggcgtttc aactgctgga cgatctgcgt gacgatcacc cggaaaccgg 5040 taaagatcgc aataaggacg cgggaaaatc gacgctggtc aaccggctgg gcgcagacgc 5100 ggcccggcaa aagctgcgcg agcatattga ttccgccgac aaacacctca cttttgcctg 5160 tccgcagggc ggcgccatcc gacagtttat gcatctgtgg tttggccatc accttgccga 5220 ctggtcaccg gtcatgaaaa tcgcctgata actcgaggag gtataaagga tgaaaaaaac 5280 cgttgtgatt ggcgcaggct ttggtggcct ggcgctggcg attcgcctgc aggcggcagg 5340 gatcccaacc gtactgctgg agcagcggga caagcccggc ggtcgggcct acgtctggca 5400 tgaccagggc tttacctttg acgccgggcc gacggtgatc accgatccta ccgcgcttga 5460 ggcgctgttc accctggccg gcaggcgcat ggaggattac gtcaggctgc tgccggtaaa 5520 acccttctac cgactctgct gggagtccgg gaagaccctc gactatgcta acgacagcgc 5580 cgagcttgag gcgcagatta cccagttcaa cccccgcgac gtcgagggct accggcgctt 5640 tctggcttac tcccaggcgg tattccagga gggatatttg cgcctcggca gcgtgccgtt 5700 cctctctttt cgcgacatgc tgcgcgccgg gccgcagctg cttaagctcc aggcgtggca 5760 gagcgtctac cagtcggttt cgcgctttat tgaggatgag catctgcggc aggccttctc 5820 gttccactcc ctgctggtag gcggcaaccc cttcaccacc tcgtccatct acaccctgat 5880 ccacgccctt gagcgggagt ggggggtctg gttccctgag ggcggcaccg gggcgctggt 5940 gaacggcatg gtgaagctgt ttaccgatct gggcggggag atcgaactca acgcccgggt 6000 cgaagagctg gtggtggccg ataaccgcgt aagccaggtc cggctggcgg atggtcggat 6060 ctttgacacc gacgccgtag cctcgaacgc tgacgtggtg aacacctata aaaagctgct 6120 cggccaccat ccggtggggc agaagcgggc ggcagcgctg gagcgcaaga gcatgagcaa 6180 ctcgctgttt gtgctctact tcggcctgaa ccagcctcat tcccagctgg cgcaccatac 6240 catctgtttt ggtccccgct accgggagct gatcgacgag atctttaccg gcagcgcgct 6300 ggcggatgac ttctcgctct acctgcactc gccctgcgtg accgatccct cgctcgcgcc 6360 tcccggctgc gccagcttct acgtgctggc cccggtgccg catcttggca acgcgccgct 6420 ggactgggcg caggaggggc cgaagctgcg cgaccgcatc tttgactacc ttgaagagcg 6480 ctatatgccc ggcctgcgta gccagctggt gacccagcgg atctttaccc cggcagactt 6540 ccacgacacg ctggatgcgc atctgggatc ggccttctcc atcgagccgc tgctgaccca 6600 aagcgcctgg ttccgcccgc acaaccgcga cagcgacatt gccaacctct acctggtggg 6660 cgcaggtact caccctgggg cgggcattcc tggcgtagtg gcctcggcga aagccaccgc 6720 cagcctgatg attgaggatc tgcaatgagc caaccgccgc tgcttgacca cgccacgcag 6780 accatggcca acggctcgaa aagttttgcc accgctgcga agctgttcga cccggccacc 6840 cgccgtagcg tgctgatgct ctacacctgg tgccgccact gcgatgacgt cattgacgac 6900 cagacccacg gcttcgccag cgaggccgcg gcggaggagg aggccaccca gcgcctggcc 6960 cggctgcgca cgctgaccct ggcggcgttt gaaggggccg agatgcagga tccggccttc 7020 gctgcctttc aggaggtggc gctgacccac ggtattacgc cccgcatggc gctcgatcac 7080 ctcgacggct ttgcgatgga cgtggctcag acccgctatg tcacctttga ggatacgctg 7140 cgctactgct atcacgtggc gggcgtggtg ggtctgatga tggccagggt gatgggcgtg 7200 cgggatgagc gggtgctgga tcgcgcctgc gatctggggc tggccttcca gctgacgaat 7260 atcgcccggg atattattga cgatgcggct attgaccgct gctatctgcc cgccgagtgg 7320 ctgcaggatg ccgggctgac cccggagaac tatgccgcgc gggagaatcg ggccgcgctg 7380 gcgcgggtgg cggagcggct tattgatgcc gcagagccgt actacatctc ctcccaggcc 7440 gggctacacg atctgccgcc gcgctgcgcc tgggcgatcg ccaccgcccg cagcgtctac 7500 cgggagatcg gtattaaggt aaaagcggcg ggaggcagcg cctgggatcg ccgccagcac 7560 accagcaaag gtgaaaaaat tgccatgctg atggcggcac cggggcaggt tattcgggcg 7620 aagacgacga gggtgacgcc gcgtccggcc ggtctttggc agcgtcccgt ttagtaatct 7680 agaggaggta ataaaatatg cttcgttcgt tgctcagagg cctcacgcat atcccccgcg 7740 tgaactccgc ccagcagccc agctgtgcac acgcgcgact ccagtttaag ctcaggagca 7800 tgcagatgac gctcatgcag cccagcatct cagccaatct gtcgcgcgcc gaggaccgca 7860 cagaccacat gaggggtgca agcacctggg caggcgggca gtcgcaggat gagctgatgc 7920 tgaaggacga gtgcatcttg gtggatgttg aggacaacat cacaggccat gccagcaagc 7980 tggagtgtca caagttccta ccacatcagc ctgcaggcct gctgcaccgg gccttctctg 8040 tgttcctgtt tgacgatcag gggcgactgc tgctgcaaca gcgtgcacgc tcaaaaatca 8100 ccttcccaag tgtgtggacg aacacctgct gcagccaccc tttacatggg cagaccccag 8160 atgaggtgga ccaactaagc caggtggccg acggaacagt acctggcgca aaggctgctg 8220 ccatccgcaa gttggagcac gagctgggga taccagcgca ccagctgccg gcaagcgcgt 8280 ttcgcttcct cacgcgtttg cactactgtg ccgcggacgt gcagccagct gcgacacaat 8340 cagcgctctg gggcgagcac gaaatggact acatcttgtt catccgggcc aacgtcacct 8400 tggcgcccaa ccctgacgag gtggacgaag tcaggtacgt gacgcaagag gagctgcggc 8460 agatgatgca gccggacaac gggctgcaat ggtcgccgtg gtttcgcatc atcgccgcgc 8520 gcttccttga gcgttggtgg gctgacctgg acgcggccct aaacactgac aaacacgagg 8580 attggggaac ggtgcatcac atcaacgaag cgtgataagc ggccgcgctg ttgacaatta 8640 atcatccggc tcgtataatg tgtggaattg tgagcggata acaatttcac acaggaaaca 8700 gaccatggag ttgaaaacag tagttattat tgatgcatta cgaacaccaa ttggaaaata 8760 taaaggcagc ttaagtcaag taagtgccgt agacttagga acacatgtta caacacaact 8820 tttaaaaaga cattccacta tttctgaaga aattgatcaa gtaatctttg gaaatgtttt 8880 acaagctgga aatggccaaa atcccgcacg acaaatagca ataaacagcg gtttatctca 8940 tgaaattccc gcaatgacag ttaatgaggt ctgcggatca ggaatgaagg ccgttatttt 9000 ggcgaaacaa ttgattcaat taggagaagc ggaagtttta attgctggcg ggattgagaa 9060 tatgtcccaa gcacctaaat tacaacgatt taattacgaa acagaaagct atgatgcgcc 9120 tttttctagt atgatgtacg atgggttaac ggatgccttt agtggtcaag caatgggctt 9180 aactgctgaa aatgtggccg aaaagtatca tgtaactaga gaagagcaag atcaattttc 9240 tgtacattca caattaaaag cagctcaagc acaagcagaa gggatattcg ctgacgaaat 9300 agccccatta gaagtatcag gaacgcttgt ggagaaagat gaagggattc gccctaattc 9360 gagcgttgag aagctaggaa cgcttaaaac agtttttaaa gaagacggta ctgtaacagc 9420 agggaatgca tcaaccatta atgatggggc ttctgctttg attattgctt cacaagaata 9480 tgccgaagca cacggtcttc cttatttagc tattattcga gacagtgtgg aagtcggtat 9540 tgatccagcc tatatgggaa tttcgccgat taaagccatt caaaaactgt tagcgcgcaa 9600 tcaacttact acggaagaaa ttgatctgta tgaaatcaac gaagcatttg cagcaacttc 9660 aatcgtggtc caaagagaac tggctttacc agaggaaaag gtcaacattt atggtggcgg 9720 tatttcatta ggtcatgcga ttggtgccac aggtgctcgt ttattaacga gtttaagtta 9780 tcaattaaat caaaaagaaa agaaatatgg agtggcttct ttatgtatcg gcggtggctt 9840 aggactcgct atgctactag agagacctca gcaaaaaaaa aacagccgat tttatcaaat 9900 gagtcctgag gaacgcctgg cttctcttct taatgaaggc cagatttctg ctgatacaaa 9960 aaaagaattt gaaaatacgg ctttatcttc gcagattgcc aatcatatga ttgaaaatca 10020 aatcagtgaa acagaagtgc cgatgggcgt tggcttacat ttaacagtgg acgaaactga 10080 ttatttggta ccaatggcga cagaagagcc ctcagtgatt gcggctttga gtaatggtgc 10140 aaaaatagca caaggattta aaacagtgaa tcaacaacgt ttaatgcgtg gacaaatcgt 10200 tttttacgat gttgcagacg ccgagtcatt gattgatgaa ctacaagtaa gagaaacgga 10260 aatttttcaa caagcagagt taagttatcc atctatcgtt aaacgcggcg gcggcttaag 10320 agatttgcaa tatcgtgctt ttgatgaatc atttgtatct gtcgactttt tagtagatgt 10380 taaggatgca atgggggcaa atatcgttaa cgctatgttg gaaggtgtgg ccgagttgtt 10440 ccgtgaatgg tttgcggagc aaaagatttt attcagtatt ttaagtaatt atgccacgga 10500 gtcggttgtt acgatgaaaa cggctattcc agtttcacgt ttaagtaagg ggagcaatgg 10560 ccgggaaatt gctgaaaaaa ttgttttagc ttcacgctat gcttcattag atccttatcg 10620 ggcagtcacg cataacaaag ggatcatgaa tggcattgaa gctgtcgttt tagctacagg 10680 aaatgataca cgcgctgtta gcgcttcttg tcatgctttt gcggtgaagg aaggtcgcta 10740 ccaaggtttg actagttgga cgctggatgg cgaacaacta attggtgaaa tttcagttcc 10800 gcttgcgtta gccacggttg gcggtgccac aaaagtctta cctaaatctc aagcagctgc 10860 tgatttgtta gcagtgacgg atgcaaaaga actaagtcga gtagtagcgg ctgttggttt 10920 ggcacaaaat ttagcggcgt tacgggcctt agtctctgaa ggaattcaaa aaggacacat 10980 ggctctacaa gcacgttctt tagcgatgac ggtcggagct actggtaaag aagttgaggc 11040 agtcgctcaa caattaaaac gtcaaaaaac gatgaaccaa gaccgagcct tggctatttt 11100 aaatgattta agaaaacaat aaaaaaacag ttcagcagaa attattctgc tgaactgttt 11160 tttttcacat taggtagccg tttcagctcg acaggaggag cggctatgca accgcattat 11220 gatctgattc tcgtgggggc tggactcgcg aatggcctta tcgccctgcg tcttcagcag 11280 cagcaacctg atatgcgtat tttgcttatc gacgccgcac cccaggcggg cgggaatcat 11340 acgtggtcat ttcaccacga tgatttgact gagagccaac atcgttggat agctccgctg 11400 gtggttcatc actggcccga ctatcaggta cgctttccca cacgccgtcg taagctgaac 11460 agcggctact tttgtattac ttctcagcgt ttcgctgagg ttttacagcg acagtttggc 11520 ccgcacttgt ggatggatac cgcggtcgca gaggttaatg cggaatctgt tcggttgaaa 11580 aagggtcagg ttatcggtgc ccgcgcggtg attgacgggc ggggttatgc ggcaaattca 11640 gcactgagcg tgggcttcca ggcgtttatt ggccaggaat ggcgattgag ccacccgcat 11700 ggtttatcgt ctcccattat catggatgcc acggtcgatc agcaaaatgg ttatcgcttc 11760 gtgtacagcc tgccgctctc gccgaccaga ttgttaattg aagacacgca ctatattgat 11820 aatgcgacat tagatcctga atgcgcgcgg caaaatattt gcgactatgc cgcgcaacag 11880 ggttggcagc ttcagacact gctgcgagaa gaacagggcg ccttacccat tactctgtcg 11940 ggcaatgccg acgcattctg gcagcagcgc cccctggcct gtagtggatt acgtgccggt 12000 ctgttccatc ctaccaccgg ctattcactg ccgctggcgg ttgccgtggc cgaccgcctg 12060 agtgcacttg atgtctttac gtcggcctca attcaccatg ccattacgca ttttgcccgc 12120 gagcgctggc agcagcaggg ctttttccgc atgctgaatc gcatgctgtt tttagccgga 12180 cccgccgatt cacgctggcg ggttatgcag cgtttttatg gtttacctga agatttaatt 12240 gcccgttttt atgcgggaaa actcacgctg accgatcggc tacgtattct gagcggcaag 12300 ccgcctgttc cggtattagc agcattgcaa gccattatga cgactcatcg ttaactgcag 12360 gcatgcaagc tggggatcct acctgacgct ttttatcgca actctctact gtttctccat 12420 acccgttttt ttgggctggc ggatgagaga agattttcag cctgatacag attaaatcag 12480 aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac 12540 ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc 12600 cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac 12660 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg 12720 ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg 12780 ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg 12840 tttctacaaa ctctttttgt ttatttttct aaatacattc aaatatgtat ccgctcaa 12898 <210> 87 <211> 5381 <212> DNA <213> Artificial Sequence <220> <223> pT-dxr <400> 87 aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg 60 atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg 120 atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa 180 ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac 240 tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa 300 aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 360 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat 420 gtgagttagc gcgaattgat ctggtttgac agcttatcat cgactgcacg gtgcaccaat 480 gcttctggcg tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact 540 gcataattcg tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat 600 cataacggtt ctggcaaata ttctgaaatg agctgttgac aattaatcat ccggctcgta 660 taatgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagacca tggaattcga 720 gctcatgaag caactcacca ttctgggctc gaccggctcg attggttgca gcacgctgga 780 cgtggtgcgc cataatcccg aacacttccg cgtagttgcg ctggtggcag gcaaaaatgt 840 cactcgcatg gtagaacagt gcctggaatt ctctccccgc tatgccgtaa tggacgatga 900 agcgagtgcg aaacttctta aaacgatgct acagcaacag ggtagccgca ccgaagtctt 960 aagtgggcaa caagccgctt gcgatatggc agcgcttgag gatgttgatc aggtgatggc 1020 agccattgtt ggcgctgctg ggctgttacc tacgcttgct gcgatccgcg cgggtaaaac 1080 cattttgctg gccaataaag aatcactggt tacctgcgga cgtctgttta tggacgccgt 1140 aaagcagagc aaagcgcaat tgttaccggt cgatagcgaa cataacgcca tttttcagag 1200 tttaccgcaa cctatccagc ataatctggg atacgctgac cttgagcaaa atggcgtggt 1260 gtccatttta cttaccgggt ctggtggccc tttccgtgag acgccattgc gcgatttggc 1320 aacaatgacg ccggatcaag cctgccgtca tccgaactgg tcgatggggc gtaaaatttc 1380 tgtcgattcg gctaccatga tgaacaaagg tctggaatac attgaagcgc gttggctgtt 1440 taacgccagc gccagccaga tggaagtgct gattcacccg cagtcagtga ttcactcaat 1500 ggtgcgctat caggacggca gtgttctggc gcagctgggg gaaccggata tgcgtacgcc 1560 aattgcccac accatggcat ggccgaatcg cgtgaactct ggcgtgaagc cgctcgattt 1620 ttgcaaacta agtgcgttga catttgccgc accggattat gatcgttatc catgcctgaa 1680 actggcgatg gaggcgttcg aacaaggcca ggcagcgacg acagcattga atgccgcaaa 1740 cgaaatcacc gttgctgctt ttcttgcgca acaaatccgc tttacggata tcgctgcgtt 1800 gaatttatcc gtactggaaa aaatggatat gcgcgaacca caatgtgtgg acgatgtgtt 1860 atctgttgat gcgaacgcgc gtgaagtcgc cagaaaagag gtgatgcgtc tcgcaagctg 1920 atctagagcg ggggatccac tagttctaga gtcgacctgc aggcatgcaa gcttggctgt 1980 tttggcggat gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt 2040 ctgataaaac agaatttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg 2100 aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta 2160 gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 2220 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt 2280 gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag 2340 gcatcaaatt aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct 2400 ttttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga 2460 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc 2520 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg 2580 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc 2640 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact 2700 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc 2760 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag 2820 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat 2880 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt 2940 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa 3000 gccataccaa acgacgagcg tgacaccacg atgcctacag caatggcaac aacgttgcgc 3060 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg 3120 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt 3180 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca 3240 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat 3300 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca 3360 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 3420 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 3480 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 3540 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 3600 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 3660 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 3720 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 3780 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 3840 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 3900 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 3960 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 4020 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 4080 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 4140 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 4200 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc 4260 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt 4320 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat 4380 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc 4440 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg 4500 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat 4560 caccgaaacg cgcgaggcag cagatcaatt cgcgcgcgaa ggcgaagcgg catgcattta 4620 cgttgacacc atcgaatggt gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga 4680 gagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc 4740 cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa 4800 aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc 4860 acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct 4920 gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag 4980 cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa 5040 tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc 5100 cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca 5160 gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca 5220 tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc 5280 ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat 5340 agcggaacgg gaaggcgact ggagtgccat gtccggtttt c 5381 <210> 88 <211> 7999 <212> DNA <213> Artificial Sequence <220> <223> pTAS-dxr <400> 88 tcgacctgca ggcatgcaag cttggctgtt ttggcggatg agagaagatt ttcagcctga 60 tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct ggcggcagta 120 gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg 180 gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag 240 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg 300 agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg 360 cgggcaggac gcccgccata aactgccagg catcaaatta agcagaaggc catcctgacg 420 gatggccttt ttgcgtttct acaaactctt tttgtttatt tttctaaata cattcaaata 480 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga 540 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc 600 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg 660 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc 720 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat 780 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact 840 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat 900 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga 960 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc 1020 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga 1080 tgcctacagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag 1140 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc 1200 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt 1260 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct 1320 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg 1380 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg 1440 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca 1500 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga 1560 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 1620 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 1680 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt 1740 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 1800 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 1860 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 1920 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 1980 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 2040 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 2100 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 2160 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 2220 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag 2280 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg 2340 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat 2400 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct 2460 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc 2520 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag 2580 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc agatcaattc 2640 gcgcgcgaag gcgaagcggc atgcatttac gttgacacca tcgaatggtg caaaaccttt 2700 cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca 2760 gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg 2820 gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg 2880 gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg 2940 attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt 3000 aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc 3060 gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc 3120 attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt 3180 ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat 3240 gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg 3300 ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa 3360 tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg 3420 tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg 3480 gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc 3540 gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc 3600 ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc 3660 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg 3720 gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc 3780 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 3840 cgcaattaat gtgagttagc gcgaattgat ctggtttgac agcttatcat cgactgcacg 3900 gtgcaccaat gcttctggcg tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc 3960 gtaaatcact gcataattcg tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt 4020 gcgccgacat cataacggtt ctggcaaata ttctgaaatg agctgttgac aattaatcat 4080 ccggctcgta taatgtgtgg aattgtgagc ggataacaat ttcacacagg aggtaataaa 4140 tatggaattc atggactttc cgcagcaact cgaagcctgc gttaagcagg ccaaccaggc 4200 gctgagccgt tttatcgccc cactgccctt tcagaacact cccgtggtcg aaaccatgca 4260 gtatggcgca ttattaggtg gtaagcgcct gcgacctttc ctggtttatg ccaccggtca 4320 tatgttcggc gttagcacaa acacgctgga cgcacccgct gccgccgttg agtgtatcca 4380 cgcttactca ttaattcatg atgatttacc ggcaatggat gatgacgatc tgcgtcgcgg 4440 tttgccaacc tgccatgtga agtttggcga agcaaacgcg attctcgctg gcgacgcttt 4500 acaaacgctg gcgttctcga ttttaagcga tgccgatatg ccggaagtgt cggaccgcga 4560 cagaatttcg atgatttctg aactggcgag cgccagtggt attgccggaa tgtgcggtgg 4620 tcaggcatta gatttagacg cggaaggcaa acacgtacct ctggacgcgc ttgagcgtat 4680 tcatcgtcat aaaaccggcg cattgattcg cgccgccgtt cgccttggtg cattaagcgc 4740 cggagataaa ggacgtcgtg ctctgccggt actcgacaag tatgcagaga gcatcggcct 4800 tgccttccag gttcaggatg acatcctgga tgtggtggga gatactgcaa cgttgggaaa 4860 acgccagggt gccgaccagc aacttggtaa aagtacctac cctgcacttc tgggtcttga 4920 gcaagcccgg aagaaagccc gggatctgat cgacgatgcc cgtcagtcgc tgaaacaact 4980 ggctgaacag tcactcgata cctcggcact ggaagcgcta gcggactaca tcatccagcg 5040 taataaataa ggatccaaaa aggaggtaat aaaccatgtc aactcaacaa gtttcatcag 5100 agaacattgt tcgtaacgct gcgaatttcc atcctaatat atggggaaac catttcctca 5160 catgtccttc tcagacgatt gatagttgga ctcaacagca ccacaaagaa ctgaaagaag 5220 aggtgaggaa aatgatggtg tctgatgcaa ataaacctgc ccagagattg cgcttgattg 5280 atactgtcca aaggctaggt gtggcttacc actttgaaaa ggagattgat gatgcattgg 5340 agaaaatagg tcatgaccct tttgatgata aagatgatct ctacattgtc tctctttgtt 5400 ttcgattgct gaggcagcat ggaattaaga tatcatgtga tgtgtttgag aagtttaaag 5460 atgacgatgg aaaattcaag gcatcattga tgaatgatgt tcaaggcatg ctaagtttat 5520 atgaggcagc acacctagcc attcacggag aagatatttt agatgaagca attgttttca 5580 cgaccactca ccttaagtca acggtatcta attctcctgt aaactctact tttgctgaac 5640 aaatacgtca ttctctcaga gttcctctcc gtaaagctgt acctaggtta gagtcgaggt 5700 atttcttgga tatctattca agagatgatt tgcacgataa aactttgctc aatttcgcaa 5760 agttagactt taatatacta caagcaatgc accagaagga agcaagtgag atgaccaggt 5820 ggtggagaga ttttgacttc cttaaaaagc tgccttatat aagagacaga gtcgtggagc 5880 tatatttttg gattctggtg ggagtgtctt atcagcccaa attcagcact ggtagaattt 5940 ttttgtccaa aataatatgc cttgagaccc tcgtagatga tacatttgac gcctacggta 6000 cttttgacga gctcgcaatc tttactgaag cagttacaag atgggacctt ggccacagag 6060 atgcactacc agaatacatg aaattcattt tcaagacact cattgatgtc tacagtgaag 6120 ctgagcaaga actggcaaag gaagggagat catacagcat acactatgca atacgatcgt 6180 tccaagaact agttatgaag tacttctgcg aagccaagtg gttaaataaa ggttatgttc 6240 cgagcctgga cgattataaa tcagtttcat taagaagtat cggtttttta ccgatagcgg 6300 tagcttcctt cgttttcatg ggtgatattg caactaagga ggtctttgaa tgggaaatga 6360 ataaccctaa gatcataata gccgcagaaa cgattttcag attcctggat gacatagcag 6420 gccataggtt tgagcaaaag agagaacata gtccatcagc tattgaatgc tacaagaatc 6480 aacatggagt gtctgaggaa gaggcagtta aagcgttgtc gttagaagtt gctaatagtt 6540 ggaaagatat aaatgaggag ctgcttctca acccaatggc tattccttta cctctgcttc 6600 aggtgattct tgatctctca cgttcggccg attttatgta cggtaatgct caagatcgct 6660 tcacgcattc aacgatgatg aaagaccaag ttgatttggt gctgaaggac cccgttaagc 6720 ttgacgatta aagatctgtg tggaattgtg agcggataac aatttcacac aggaaacaga 6780 ccatggaatt cgagctcatg aagcaactca ccattctggg ctcgaccggc tcgattggtt 6840 gcagcacgct ggacgtggtg cgccataatc ccgaacactt ccgcgtagtt gcgctggtgg 6900 caggcaaaaa tgtcactcgc atggtagaac agtgcctgga attctctccc cgctatgccg 6960 taatggacga tgaagcgagt gcgaaacttc ttaaaacgat gctacagcaa cagggtagcc 7020 gcaccgaagt cttaagtggg caacaagccg cttgcgatat ggcagcgctt gaggatgttg 7080 atcaggtgat ggcagccatt gttggcgctg ctgggctgtt acctacgctt gctgcgatcc 7140 gcgcgggtaa aaccattttg ctggccaata aagaatcact ggttacctgc ggacgtctgt 7200 ttatggacgc cgtaaagcag agcaaagcgc aattgttacc ggtcgatagc gaacataacg 7260 ccatttttca gagtttaccg caacctatcc agcataatct gggatacgct gaccttgagc 7320 aaaatggcgt ggtgtccatt ttacttaccg ggtctggtgg ccctttccgt gagacgccat 7380 tgcgcgattt ggcaacaatg acgccggatc aagcctgccg tcatccgaac tggtcgatgg 7440 ggcgtaaaat ttctgtcgat tcggctacca tgatgaacaa aggtctggaa tacattgaag 7500 cgcgttggct gtttaacgcc agcgccagcc agatggaagt gctgattcac ccgcagtcag 7560 tgattcactc aatggtgcgc tatcaggacg gcagtgttct ggcgcagctg ggggaaccgg 7620 atatgcgtac gccaattgcc cacaccatgg catggccgaa tcgcgtgaac tctggcgtga 7680 agccgctcga tttttgcaaa ctaagtgcgt tgacatttgc cgcaccggat tatgatcgtt 7740 atccatgcct gaaactggcg atggaggcgt tcgaacaagg ccaggcagcg acgacagcat 7800 tgaatgccgc aaacgaaatc accgttgctg cttttcttgc gcaacaaatc cgctttacgg 7860 atatcgctgc gttgaattta tccgtactgg aaaaaatgga tatgcgcgaa ccacaatgtg 7920 tggacgatgt gttatctgtt gatgcgaacg cgcgtgaagt cgccagaaaa gaggtgatgc 7980 gtctcgcaag ctgatctac 7999 <210> 89 <211> 7275 <212> DNA <213> Artificial Sequence <220> <223> pT-dxr/s <400> 89 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc 60 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc 120 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc 180 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga 240 taacaatttc acacaggaaa cagaccatgg aattcatgag ttttgatatt gccaaatacc 300 cgaccctggc actggtcgac tccacccagg agttacgact gttgccgaaa gagagtttac 360 cgaaactctg cgacgaactg cgccgctatt tactcgacag cgtgagccgt tccagcgggc 420 acttcgcctc cgggctgggc acggtcgaac tgaccgtggc gctgcactat gtctacaaca 480 ccccgtttga ccaattgatt tgggatgtgg ggcatcaggc ttatccgcat aaaattttga 540 ccggacgccg cgacaaaatc ggcaccatcc gtcagaaagg cggtctgcac ccgttcccgt 600 ggcgcggcga aagcgaatat gacgtattaa gcgtcgggca ttcatcaacc tccatcagtg 660 ccggaattgg tattgcggtt gctgccgaaa aagaaggcaa aaatcgccgc accgtctgtg 720 tcattggcga tggcgcgatt accgcaggca tggcgtttga agcgatgaat cacgcgggcg 780 atatccgtcc tgatatgctg gtgattctca acgacaatga aatgtcgatt tccgaaaatg 840 tcggcgcgct caacaaccat ctggcacagc tgctttccgg taagctttac tcttcactgc 900 gcgaaggcgg gaaaaaagtt ttctctggcg tgccgccaat taaagagctg ctcaaacgca 960 ccgaagaaca tattaaaggc atggtagtgc ctggcacgtt gtttgaagag ctgggcttta 1020 actacatcgg cccggtggac ggtcacgatg tgctggggct tatcaccacg ctaaagaaca 1080 tgcgcgacct gaaaggcccg cagttcctgc atatcatgac caaaaaaggt cgtggttatg 1140 aaccggcaga aaaagacccg atcactttcc acgccgtgcc taaatttgat ccctccagcg 1200 gttgtttgcc gaaaagtagc ggcggtttgc cgagctattc aaaaatcttt ggcgactggt 1260 tgtgcgaaac ggcagcgaaa gacaacaagc tgatggcgat tactccggcg atgcgtgaag 1320 gttccggcat ggtcgagttt tcacgtaaat tcccggatcg ctacttcgac gtggcaattg 1380 ccgagcaaca cgcggtgacc tttgctgcgg gtctggcgat tggtgggtac aaacccattg 1440 tcgcgattta ctccactttc ctgcaacgcg cctatgatca ggtgctgcat gacgtggcga 1500 ttcaaaagct tccggtcctg ttcgccatcg accgcgcggg cattgttggt gctgacggtc 1560 aaacccatca gggtgctttt gatctctctt acctgcgctg cataccggaa atggtcatta 1620 tgaccccgag cgatgaaaac gaatgtcgcc agatgctcta taccggctat cactataacg 1680 atggcccgtc agcggtgcgc tacccgcgtg gcaacgcggt cggcgtggaa ctgacgccgc 1740 tggaaaaact accaattggc aaaggcattg tgaagcgtcg tggcgagaaa ctggcgatcc 1800 ttaactttgg tacgctgatg ccagaagcgg cgaaagtcgc cgaatcgctg aacgccacgc 1860 tggtcgatat gcgttttgtg aaaccgcttg atgaagcgtt aattctggaa atggccgcca 1920 gccatgaagc gctggtcacc gtagaagaaa acgccattat gggcggcgca ggcagcggcg 1980 tgaacgaagt gctgatggcc catcgtaaac cagtacccgt gctgaacatt ggcctgccgg 2040 acttctttat tccgcaagga actcaggaag aaatgcgcgc cgaactcggc ctcgatgccg 2100 ctggtatgga agccaaaatc aaggcctggc tggcataagg taccacacag gaaacagacc 2160 atggaattcg agctcatgaa gcaactcacc attctgggct cgaccggctc gattggttgc 2220 agcacgctgg acgtggtgcg ccataatccc gaacacttcc gcgtagttgc gctggtggca 2280 ggcaaaaatg tcactcgcat ggtagaacag tgcctggaat tctctccccg ctatgccgta 2340 atggacgatg aagcgagtgc gaaacttctt aaaacgatgc tacagcaaca gggtagccgc 2400 accgaagtct taagtgggca acaagccgct tgcgatatgg cagcgcttga ggatgttgat 2460 caggtgatgg cagccattgt tggcgctgct gggctgttac ctacgcttgc tgcgatccgc 2520 gcgggtaaaa ccattttgct ggccaataaa gaatcactgg ttacctgcgg acgtctgttt 2580 atggacgccg taaagcagag caaagcgcaa ttgttaccgg tcgatagcga acataacgcc 2640 atttttcaga gtttaccgca acctatccag cataatctgg gatacgctga ccttgagcaa 2700 aatggcgtgg tgtccatttt acttaccggg tctggtggcc ctttccgtga gacgccattg 2760 cgcgatttgg caacaatgac gccggatcaa gcctgccgtc atccgaactg gtcgatgggg 2820 cgtaaaattt ctgtcgattc ggctaccatg atgaacaaag gtctggaata cattgaagcg 2880 cgttggctgt ttaacgccag cgccagccag atggaagtgc tgattcaccc gcagtcagtg 2940 attcactcaa tggtgcgcta tcaggacggc agtgttctgg cgcagctggg ggaaccggat 3000 atgcgtacgc caattgccca caccatggca tggccgaatc gcgtgaactc tggcgtgaag 3060 ccgctcgatt tttgcaaact aagtgcgttg acatttgccg caccggatta tgatcgttat 3120 ccatgcctga aactggcgat ggaggcgttc gaacaaggcc aggcagcgac gacagcattg 3180 aatgccgcaa acgaaatcac cgttgctgct tttcttgcgc aacaaatccg ctttacggat 3240 atcgctgcgt tgaatttatc cgtactggaa aaaatggata tgcgcgaacc acaatgtgtg 3300 gacgatgtgt tatctgttga tgcgaacgcg cgtgaagtcg ccagaaaaga ggtgatgcgt 3360 ctcgcaagct gatctagagc gggggatcca ctagttctag agtcgacctg caggcatgca 3420 agcttggctg ttttggcgga tgagagaaga ttttcagcct gatacagatt aaatcagaac 3480 gcagaagcgg tctgataaaa cagaatttgc ctggcggcag tagcgcggtg gtcccacctg 3540 accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc 3600 atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 3660 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg 3720 ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca 3780 taaactgcca ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt 3840 ctacaaactc tttttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac 3900 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt 3960 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag 4020 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg 4080 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa 4140 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc 4200 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag 4260 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa 4320 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc 4380 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg 4440 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctaca gcaatggcaa 4500 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa 4560 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg 4620 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag 4680 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg 4740 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt 4800 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt 4860 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac 4920 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 4980 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 5040 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 5100 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga 5160 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 5220 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 5280 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 5340 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 5400 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 5460 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 5520 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 5580 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 5640 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca 5700 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt 5760 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa 5820 tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt 5880 catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct 5940 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt 6000 ttcaccgtca tcaccgaaac gcgcgaggca gcagatcaat tcgcgcgcga aggcgaagcg 6060 gcatgcattt acgttgacac catcgaatgg tgcaaaacct ttcgcggtat ggcatgatag 6120 cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc 6180 gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac 6240 gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc 6300 aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc 6360 agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa 6420 ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg 6480 gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat 6540 gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat 6600 gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg tacgcgactg 6660 ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta 6720 agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa 6780 attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc 6840 atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg 6900 gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggatatctcg 6960 gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt aaccaccatc 7020 aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag 7080 ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc 7140 ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg 7200 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta 7260 gcgcgaattg atctg 7275 <210> 90 <211> 6798 <212> DNA <213> Artificial Sequence <220> <223> pT-ispA-MrBBS <400> 90 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc 60 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc 120 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc 180 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga 240 taacaatttc acacaggaaa cagaccatgg aattcatgga ctttccgcag caactcgaag 300 cctgcgttaa gcaggccaac caggcgctga gccgttttat cgccccactg ccctttcaga 360 acactcccgt ggtcgaaacc atgcagtatg gcgcattatt aggtggtaag cgcctgcgac 420 ctttcctggt ttatgccacc ggtcatatgt tcggcgttag cacaaacacg ctggacgcac 480 ccgctgccgc cgttgagtgt atccacgctt actcattaat tcatgatgat ttaccggcaa 540 tggatgatga cgatctgcgt cgcggtttgc caacctgcca tgtgaagttt ggcgaagcaa 600 acgcgattct cgctggcgac gctttacaaa cgctggcgtt ctcgatttta agcgatgccg 660 atatgccgga agtgtcggac cgcgacagaa tttcgatgat ttctgaactg gcgagcgcca 720 gtggtattgc cggaatgtgc ggtggtcagg cattagattt agacgcggaa ggcaaacacg 780 tacctctgga cgcgcttgag cgtattcatc gtcataaaac cggcgcattg attcgcgccg 840 ccgttcgcct tggtgcatta agcgccggag ataaaggacg tcgtgctctg ccggtactcg 900 acaagtatgc agagagcatc ggccttgcct tccaggttca ggatgacatc ctggatgtgg 960 tgggagatac tgcaacgttg ggaaaacgcc agggtgccga ccagcaactt ggtaaaagta 1020 cctaccctgc acttctgggt cttgagcaag cccggaagaa agcccgggat ctgatcgacg 1080 atgcccgtca gtcgctgaaa caactggctg aacagtcact cgatacctcg gcactggaag 1140 cgctagcgga ctacatcatc cagcgtaata aataaggatc caaggagata tatcaaatgt 1200 caaccctgtc agtctccacg ccgtccttct catcgtcgcc gctgtcctca gtgaacaaaa 1260 atagcacgaa acaacacgtt acgcgcaata gtgtgatctt tcatgattcc atttggggcg 1320 accagttcct ggaatacaaa gaaaaattca acgttgcaac cgaaaaacaa ctgattgaag 1380 aactgaaaga agaagtccgc aatgaactga tgatccgtgc gtgcaacgaa gccagtcgct 1440 atattaaact gatccagctg attgatgtgg ttgaacgtct gggcctggcc taccactttg 1500 aaaaagaaat cgaagaatcc ctgcaacata tttatgttac ctacggtcac aaatggacga 1560 actacaacaa catcgaaagc ctgtctctgt ggtttcgcct gctgcgtcag aacggtttta 1620 atgtcagctc tgatatcttc gaaaaccata ttgacgaaaa aggcaatttc caagaaagtc 1680 tgtgcaacga tccgcaaggc atgctggcac tgtatgaagc ggcctacatg cgcgtggaag 1740 gcgaaattat cctggacaaa gctctggaat ttaccaaact gcacctgggt attatcagca 1800 acgatccgtc ttgtgacagt tccctgcgta cggaaattaa acaggcgctg aaacagccgc 1860 tgcgtcgccg tctgccgcgt ctggaagcag ttcgttatat tgctatctac cagcaaaaag 1920 cgagtcattc cgaagtcctg ctgaaactgg ccaaactgga tttcaatgtg ctgcaggaaa 1980 tgcacaaaga cgaactgtca caaatttgta aatggtggaa agacctggac atccgtaaca 2040 aactgccgta tgttcgcgat cgtctgattg aaggctattt ttggattctg ggtatttact 2100 tcgaaccgca gcattcgcgc acccgtatgt ttctgatgaa aacgtgcatg tggctgatcg 2160 tcctggatga cacctttgat aattatggca cgtacgaaga actggaaatt ttcacccagg 2220 cggtggaacg ctggagcatc acgtgtctgg atgaactgcc ggaatacatg aaactgatct 2280 accatgaaca gttccgtgtg caccaagaaa tggaagaatc tctggaaaaa gaaggtaaag 2340 cataccagat ccattacatc aaagaaatgg ctaaagaagg cacccgctct ctgctgctgg 2400 aagcgaaatg gctgaaagaa ggttatatgc cgacgctgga tgaatacctg tcaaactcgc 2460 tggttacctg cggctatgca ctgatgacgg ctcgctcata cgttgcccgt gatgacggta 2520 ttgtcaccga agatgcgttt aaatgggtgg ccacgcaccc gccgatcgtt aaagcagctt 2580 gtaaaattct gcgtctgatg gatgacatcg cgacccataa agaagaacag gaacgcggcc 2640 acatcgcctc atcgattgaa tgctatcgta aagaaaccgg tgcatctgaa gaagaagcgt 2700 gtatggattt tctgaaacag gtcgaagacg gctggaaagt gatcaatcaa gaatcactga 2760 tgccgaccga tgtgccgttc ccgctgctga tcccggcgat taacctggcc cgcgtttcgg 2820 acacgctgta taaagataac gacggttaca atcatgctga taaagaagtg attggttaca 2880 ttaaaagtct gtttgtccac ccgatgattg tgtgaagatc ttaggtcgac ctgcaggcat 2940 gcaagcttgg ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag 3000 aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac 3060 ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc 3120 cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac 3180 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg 3240 ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg 3300 ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg 3360 tttctacaaa ctctttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 3420 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 3480 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 3540 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 3600 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 3660 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg 3720 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 3780 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 3840 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 3900 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 3960 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct acagcaatgg 4020 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 4080 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 4140 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 4200 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 4260 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 4320 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 4380 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 4440 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 4500 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 4560 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 4620 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca 4680 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 4740 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 4800 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 4860 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 4920 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 4980 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 5040 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 5100 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 5160 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 5220 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc 5280 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta 5340 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg 5400 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct 5460 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag 5520 gttttcaccg tcatcaccga aacgcgcgag gcagcagatc aattcgcgcg cgaaggcgaa 5580 gcggcatgca tttacgttga caccatcgaa tggtgcaaaa cctttcgcgg tatggcatga 5640 tagcgcccgg aagagagtca attcagggtg gtgaatgtga aaccagtaac gttatacgat 5700 gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc 5760 cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt 5820 cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc 5880 tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat 5940 caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa 6000 gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg 6060 gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt 6120 gatgtctctg accagacacc catcaacagt attattttct cccatgaaga cggtacgcga 6180 ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca 6240 ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat 6300 caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa 6360 accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag 6420 atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc 6480 tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc 6540 atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct 6600 cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc 6660 accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 6720 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag 6780 ttagcgcgaa ttgatctg 6798 <210> 91 <211> 8655 <212> DNA <213> Artificial Sequence <220> <223> pTAS-dxs <400> 91 tcgacctgca ggcatgcaag cttggctgtt ttggcggatg agagaagatt ttcagcctga 60 tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct ggcggcagta 120 gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg 180 gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag 240 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg 300 agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg 360 cgggcaggac gcccgccata aactgccagg catcaaatta agcagaaggc catcctgacg 420 gatggccttt ttgcgtttct acaaactctt tttgtttatt tttctaaata cattcaaata 480 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga 540 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc 600 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg 660 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc 720 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat 780 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact 840 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat 900 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga 960 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc 1020 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga 1080 tgcctacagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag 1140 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc 1200 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt 1260 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct 1320 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg 1380 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg 1440 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca 1500 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga 1560 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 1620 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 1680 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt 1740 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 1800 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 1860 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 1920 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 1980 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 2040 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 2100 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 2160 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 2220 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag 2280 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg 2340 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat 2400 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct 2460 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc 2520 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag 2580 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc agatcaattc 2640 gcgcgcgaag gcgaagcggc atgcatttac gttgacacca tcgaatggtg caaaaccttt 2700 cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca 2760 gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg 2820 gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg 2880 gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg 2940 attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt 3000 aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc 3060 gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc 3120 attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt 3180 ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat 3240 gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg 3300 ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa 3360 tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg 3420 tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg 3480 gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc 3540 gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc 3600 ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc 3660 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg 3720 gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc 3780 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 3840 cgcaattaat gtgagttagc gcgaattgat ctggtttgac agcttatcat cgactgcacg 3900 gtgcaccaat gcttctggcg tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc 3960 gtaaatcact gcataattcg tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt 4020 gcgccgacat cataacggtt ctggcaaata ttctgaaatg agctgttgac aattaatcat 4080 ccggctcgta taatgtgtgg aattgtgagc ggataacaat ttcacacagg aggtaataaa 4140 tatggaattc atggactttc cgcagcaact cgaagcctgc gttaagcagg ccaaccaggc 4200 gctgagccgt tttatcgccc cactgccctt tcagaacact cccgtggtcg aaaccatgca 4260 gtatggcgca ttattaggtg gtaagcgcct gcgacctttc ctggtttatg ccaccggtca 4320 tatgttcggc gttagcacaa acacgctgga cgcacccgct gccgccgttg agtgtatcca 4380 cgcttactca ttaattcatg atgatttacc ggcaatggat gatgacgatc tgcgtcgcgg 4440 tttgccaacc tgccatgtga agtttggcga agcaaacgcg attctcgctg gcgacgcttt 4500 acaaacgctg gcgttctcga ttttaagcga tgccgatatg ccggaagtgt cggaccgcga 4560 cagaatttcg atgatttctg aactggcgag cgccagtggt attgccggaa tgtgcggtgg 4620 tcaggcatta gatttagacg cggaaggcaa acacgtacct ctggacgcgc ttgagcgtat 4680 tcatcgtcat aaaaccggcg cattgattcg cgccgccgtt cgccttggtg cattaagcgc 4740 cggagataaa ggacgtcgtg ctctgccggt actcgacaag tatgcagaga gcatcggcct 4800 tgccttccag gttcaggatg acatcctgga tgtggtggga gatactgcaa cgttgggaaa 4860 acgccagggt gccgaccagc aacttggtaa aagtacctac cctgcacttc tgggtcttga 4920 gcaagcccgg aagaaagccc gggatctgat cgacgatgcc cgtcagtcgc tgaaacaact 4980 ggctgaacag tcactcgata cctcggcact ggaagcgcta gcggactaca tcatccagcg 5040 taataaataa ggatccaaaa aggaggtaat aaaccatgtc aactcaacaa gtttcatcag 5100 agaacattgt tcgtaacgct gcgaatttcc atcctaatat atggggaaac catttcctca 5160 catgtccttc tcagacgatt gatagttgga ctcaacagca ccacaaagaa ctgaaagaag 5220 aggtgaggaa aatgatggtg tctgatgcaa ataaacctgc ccagagattg cgcttgattg 5280 atactgtcca aaggctaggt gtggcttacc actttgaaaa ggagattgat gatgcattgg 5340 agaaaatagg tcatgaccct tttgatgata aagatgatct ctacattgtc tctctttgtt 5400 ttcgattgct gaggcagcat ggaattaaga tatcatgtga tgtgtttgag aagtttaaag 5460 atgacgatgg aaaattcaag gcatcattga tgaatgatgt tcaaggcatg ctaagtttat 5520 atgaggcagc acacctagcc attcacggag aagatatttt agatgaagca attgttttca 5580 cgaccactca ccttaagtca acggtatcta attctcctgt aaactctact tttgctgaac 5640 aaatacgtca ttctctcaga gttcctctcc gtaaagctgt acctaggtta gagtcgaggt 5700 atttcttgga tatctattca agagatgatt tgcacgataa aactttgctc aatttcgcaa 5760 agttagactt taatatacta caagcaatgc accagaagga agcaagtgag atgaccaggt 5820 ggtggagaga ttttgacttc cttaaaaagc tgccttatat aagagacaga gtcgtggagc 5880 tatatttttg gattctggtg ggagtgtctt atcagcccaa attcagcact ggtagaattt 5940 ttttgtccaa aataatatgc cttgagaccc tcgtagatga tacatttgac gcctacggta 6000 cttttgacga gctcgcaatc tttactgaag cagttacaag atgggacctt ggccacagag 6060 atgcactacc agaatacatg aaattcattt tcaagacact cattgatgtc tacagtgaag 6120 ctgagcaaga actggcaaag gaagggagat catacagcat acactatgca atacgatcgt 6180 tccaagaact agttatgaag tacttctgcg aagccaagtg gttaaataaa ggttatgttc 6240 cgagcctgga cgattataaa tcagtttcat taagaagtat cggtttttta ccgatagcgg 6300 tagcttcctt cgttttcatg ggtgatattg caactaagga ggtctttgaa tgggaaatga 6360 ataaccctaa gatcataata gccgcagaaa cgattttcag attcctggat gacatagcag 6420 gccataggtt tgagcaaaag agagaacata gtccatcagc tattgaatgc tacaagaatc 6480 aacatggagt gtctgaggaa gaggcagtta aagcgttgtc gttagaagtt gctaatagtt 6540 ggaaagatat aaatgaggag ctgcttctca acccaatggc tattccttta cctctgcttc 6600 aggtgattct tgatctctca cgttcggccg attttatgta cggtaatgct caagatcgct 6660 tcacgcattc aacgatgatg aaagaccaag ttgatttggt gctgaaggac cccgttaagc 6720 ttgacgatta aagatctgtg tggaattgtg agcggataac aatttcacac aggaaacaga 6780 ccatggaatt catgagtttt gatattgcca aatacccgac cctggcactg gtcgactcca 6840 cccaggagtt acgactgttg ccgaaagaga gtttaccgaa actctgcgac gaactgcgcc 6900 gctatttact cgacagcgtg agccgttcca gcgggcactt cgcctccggg ctgggcacgg 6960 tcgaactgac cgtggcgctg cactatgtct acaacacccc gtttgaccaa ttgatttggg 7020 atgtggggca tcaggcttat ccgcataaaa ttttgaccgg acgccgcgac aaaatcggca 7080 ccatccgtca gaaaggcggt ctgcacccgt tcccgtggcg cggcgaaagc gaatatgacg 7140 tattaagcgt cgggcattca tcaacctcca tcagtgccgg aattggtatt gcggttgctg 7200 ccgaaaaaga aggcaaaaat cgccgcaccg tctgtgtcat tggcgatggc gcgattaccg 7260 caggcatggc gtttgaagcg atgaatcacg cgggcgatat ccgtcctgat atgctggtga 7320 ttctcaacga caatgaaatg tcgatttccg aaaatgtcgg cgcgctcaac aaccatctgg 7380 cacagctgct ttccggtaag ctttactctt cactgcgcga aggcgggaaa aaagttttct 7440 ctggcgtgcc gccaattaaa gagctgctca aacgcaccga agaacatatt aaaggcatgg 7500 tagtgcctgg cacgttgttt gaagagctgg gctttaacta catcggcccg gtggacggtc 7560 acgatgtgct ggggcttatc accacgctaa agaacatgcg cgacctgaaa ggcccgcagt 7620 tcctgcatat catgaccaaa aaaggtcgtg gttatgaacc ggcagaaaaa gacccgatca 7680 ctttccacgc cgtgcctaaa tttgatccct ccagcggttg tttgccgaaa agtagcggcg 7740 gtttgccgag ctattcaaaa atctttggcg actggttgtg cgaaacggca gcgaaagaca 7800 acaagctgat ggcgattact ccggcgatgc gtgaaggttc cggcatggtc gagttttcac 7860 gtaaattccc ggatcgctac ttcgacgtgg caattgccga gcaacacgcg gtgacctttg 7920 ctgcgggtct ggcgattggt gggtacaaac ccattgtcgc gatttactcc actttcctgc 7980 aacgcgccta tgatcaggtg ctgcatgacg tggcgattca aaagcttccg gtcctgttcg 8040 ccatcgaccg cgcgggcatt gttggtgctg acggtcaaac ccatcagggt gcttttgatc 8100 tctcttacct gcgctgcata ccggaaatgg tcattatgac cccgagcgat gaaaacgaat 8160 gtcgccagat gctctatacc ggctatcact ataacgatgg cccgtcagcg gtgcgctacc 8220 cgcgtggcaa cgcggtcggc gtggaactga cgccgctgga aaaactacca attggcaaag 8280 gcattgtgaa gcgtcgtggc gagaaactgg cgatccttaa ctttggtacg ctgatgccag 8340 aagcggcgaa agtcgccgaa tcgctgaacg ccacgctggt cgatatgcgt tttgtgaaac 8400 cgcttgatga agcgttaatt ctggaaatgg ccgccagcca tgaagcgctg gtcaccgtag 8460 aagaaaacgc cattatgggc ggcgcaggca gcggcgtgaa cgaagtgctg atggcccatc 8520 gtaaaccagt acccgtgctg aacattggcc tgccggactt ctttattccg caaggaactc 8580 aggaagaaat gcgcgccgaa ctcggcctcg atgccgctgg tatggaagcc aaaatcaagg 8640 cctggctggc ataac 8655 <210> 92 <211> 8656 <212> DNA <213> Artificial Sequence <220> <223> pTAB-idi-dxr <400> 92 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc 60 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc 120 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc 180 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga 240 taacaatttc acacaggaaa cagaccatgg aattcgagct cggtacccgg ggatccgatc 300 cgggaggttt tcagtaatgg actttccgca gcaactcgaa gcctgcgtta agcaggccaa 360 ccaggcgctg agccgtttta tcgccccact gccctttcag aacactcccg tggtcgaaac 420 catgcagtat ggcgcattat taggtggtaa gcgcctgcga cctttcctgg tttatgccac 480 cggtcatatg ttcggcgtta gcacaaacac gctggacgca cccgctgccg ccgttgagtg 540 tatccacgct tactcattaa ttcatgatga tttaccggca atggatgatg acgatctgcg 600 tcgcggtttg ccaacctgcc atgtgaagtt tggcgaagca aacgcgattc tcgctggcga 660 cgctttacaa acgctggcgt tctcgatttt aagcgatgcc gatatgccgg aagtgtcgga 720 ccgcgacaga atttcgatga tttctgaact ggcgagcgcc agtggtattg ccggaatgtg 780 cggtggtcag gcattagatt tagacgcgga aggcaaacac gtacctctgg acgcgcttga 840 gcgtattcat cgtcataaaa ccggcgcatt gattcgcgcc gccgttcgcc ttggtgcatt 900 aagcgccgga gataaaggac gtcgtgctct gccggtactc gacaagtatg cagagagcat 960 cggccttgcc ttccaggttc aggatgacat cctggatgtg gtgggagata ctgcaacgtt 1020 gggaaaacgc cagggtgccg accagcaact tggtaaaagt acctaccctg cacttctggg 1080 tcttgagcaa gcccggaaga aagcccggga tctgatcgac gatgcccgtc agtcgctgaa 1140 acaactggct gaacagtcac tcgatacctc ggcactggaa gcgctagcgg actacatcat 1200 ccagcgtaat aaataagatc cataaaggag gtaagaatgt caaccctgtc agtctccacg 1260 ccgtccttct catcgtcgcc gctgtcctca gtgaacaaaa atagcacgaa acaacacgtt 1320 acgcgcaata gtgtgatctt tcatgattcc atttggggcg accagttcct ggaatacaaa 1380 gaaaaattca acgttgcaac cgaaaaacaa ctgattgaag aactgaaaga agaagtccgc 1440 aatgaactga tgatccgtgc gtgcaacgaa gccagtcgct atattaaact gatccagctg 1500 attgatgtgg ttgaacgtct gggcctggcc taccactttg aaaaagaaat cgaagaatcc 1560 ctgcaacata tttatgttac ctacggtcac aaatggacga actacaacaa catcgaaagc 1620 ctgtctctgt ggtttcgcct gctgcgtcag aacggtttta atgtcagctc tgatatcttc 1680 gaaaaccata ttgacgaaaa aggcaatttc caagaaagtc tgtgcaacga tccgcaaggc 1740 atgctggcac tgtatgaagc ggcctacatg cgcgtggaag gcgaaattat cctggacaaa 1800 gctctggaat ttaccaaact gcacctgggt attatcagca acgatccgtc ttgtgacagt 1860 tccctgcgta cggaaattaa acaggcgctg aaacagccgc tgcgtcgccg tctgccgcgt 1920 ctggaagcag ttcgttatat tgctatctac cagcaaaaag cgagtcattc cgaagtcctg 1980 ctgaaactgg ccaaactgga tttcaatgtg ctgcaggaaa tgcacaaaga cgaactgtca 2040 caaatttgta aatggtggaa agacctggac atccgtaaca aactgccgta tgttcgcgat 2100 cgtctgattg aaggctattt ttggattctg ggtatttact tcgaaccgca gcattcgcgc 2160 acccgtatgt ttctgatgaa aacgtgcatg tggctgatcg tcctggatga cacctttgat 2220 aattatggca cgtacgaaga actggaaatt ttcacccagg cggtggaacg ctggagcatc 2280 acgtgtctgg atgaactgcc ggaatacatg aaactgatct accatgaaca gttccgtgtg 2340 caccaagaaa tggaagaatc tctggaaaaa gaaggtaaag cataccagat ccattacatc 2400 aaagaaatgg ctaaagaagg cacccgctct ctgctgctgg aagcgaaatg gctgaaagaa 2460 ggttatatgc cgacgctgga tgaatacctg tcaaactcgc tggttacctg cggctatgca 2520 ctgatgacgg ctcgctcata cgttgcccgt gatgacggta ttgtcaccga agatgcgttt 2580 aaatgggtgg ccacgcaccc gccgatcgtt aaagcagctt gtaaaattct gcgtctgatg 2640 gatgacatcg cgacccataa agaagaacag gaacgcggcc acatcgcctc atcgattgaa 2700 tgctatcgta aagaaaccgg tgcatctgaa gaagaagcgt gtatggattt tctgaaacag 2760 gtcgaagacg gctggaaagt gatcaatcaa gaatcactga tgccgaccga tgtgccgttc 2820 ccgctgctga tcccggcgat taacctggcc cgcgtttcgg acacgctgta taaagataac 2880 gacggttaca atcatgctga taaagaagtg attggttaca ttaaaagtct gtttgtccac 2940 ccgatgattg tgtgaagatc ctgaggaggt aacgtatgca aacggaacac gtcattttat 3000 tgaatgcaca gggagttccc acgggtacgc tggaaaagta tgccgcacac acggcagaca 3060 cccgcttaca tctcgcgttc tccagttggc tgtttaatgc caaaggacaa ttattagtta 3120 cccgccgcgc actgagcaaa aaagcatggc ctggcgtgtg gactaactcg gtttgtgggc 3180 acccacaact gggagaaagc aacgaagacg cagtgatccg ccgttgccgt tatgagcttg 3240 gcgtggaaat tacgcctcct gaatctatct atcctgactt tcgctaccgc gccaccgatc 3300 cgagtggcat tgtggaaaat gaagtgtgtc cggtatttgc cgcacgcacc actagtgcgt 3360 tacagatcaa tgatgatgaa gtgatggatt atcaatggtg tgatttagca gatgtattac 3420 acggtattga tgccacgccg tgggcgttca gtccgtggat ggtgatgcag gcgacaaatc 3480 gcgaagccag aaaacgatta tctgcattta cccagcttaa ataagatctt agagtcgacg 3540 tgtggaattg tgagcggata acaatttcac acaggaaaca gaccatggaa ttcgagctca 3600 tgaagcaact caccattctg ggctcgaccg gctcgattgg ttgcagcacg ctggacgtgg 3660 tgcgccataa tcccgaacac ttccgcgtag ttgcgctggt ggcaggcaaa aatgtcactc 3720 gcatggtaga acagtgcctg gaattctctc cccgctatgc cgtaatggac gatgaagcga 3780 gtgcgaaact tcttaaaacg atgctacagc aacagggtag ccgcaccgaa gtcttaagtg 3840 ggcaacaagc cgcttgcgat atggcagcgc ttgaggatgt tgatcaggtg atggcagcca 3900 ttgttggcgc tgctgggctg ttacctacgc ttgctgcgat ccgcgcgggt aaaaccattt 3960 tgctggccaa taaagaatca ctggttacct gcggacgtct gtttatggac gccgtaaagc 4020 agagcaaagc gcaattgtta ccggtcgata gcgaacataa cgccattttt cagagtttac 4080 cgcaacctat ccagcataat ctgggatacg ctgaccttga gcaaaatggc gtggtgtcca 4140 ttttacttac cgggtctggt ggccctttcc gtgagacgcc attgcgcgat ttggcaacaa 4200 tgacgccgga tcaagcctgc cgtcatccga actggtcgat ggggcgtaaa atttctgtcg 4260 attcggctac catgatgaac aaaggtctgg aatacattga agcgcgttgg ctgtttaacg 4320 ccagcgccag ccagatggaa gtgctgattc acccgcagtc agtgattcac tcaatggtgc 4380 gctatcagga cggcagtgtt ctggcgcagc tgggggaacc ggatatgcgt acgccaattg 4440 cccacaccat ggcatggccg aatcgcgtga actctggcgt gaagccgctc gatttttgca 4500 aactaagtgc gttgacattt gccgcaccgg attatgatcg ttatccatgc ctgaaactgg 4560 cgatggaggc gttcgaacaa ggccaggcag cgacgacagc attgaatgcc gcaaacgaaa 4620 tcaccgttgc tgcttttctt gcgcaacaaa tccgctttac ggatatcgct gcgttgaatt 4680 tatccgtact ggaaaaaatg gatatgcgcg aaccacaatg tgtggacgat gtgttatctg 4740 ttgatgcgaa cgcgcgtgaa gtcgccagaa aagaggtgat gcgtctcgca agctgatcta 4800 aagcttggct gttttggcgg atgagagaag attttcagcc tgatacagat taaatcagaa 4860 cgcagaagcg gtctgataaa acagaatttg cctggcggca gtagcgcggt ggtcccacct 4920 gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc 4980 catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg 5040 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc 5100 gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc 5160 ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttgcgtt 5220 tctacaaact ctttttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga 5280 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat 5340 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca 5400 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc 5460 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca 5520 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg 5580 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca 5640 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata 5700 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag 5760 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg 5820 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctac agcaatggca 5880 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta 5940 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct 6000 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca 6060 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag 6120 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat 6180 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt 6240 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 6300 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 6360 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 6420 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 6480 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 6540 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 6600 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 6660 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 6720 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 6780 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 6840 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 6900 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 6960 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 7020 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 7080 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg 7140 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca 7200 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg 7260 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc 7320 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt 7380 tttcaccgtc atcaccgaaa cgcgcgaggc agcagatcaa ttcgcgcgcg aaggcgaagc 7440 ggcatgcatt tacgttgaca ccatcgaatg gtgcaaaacc tttcgcggta tggcatgata 7500 gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt 7560 cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca 7620 cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc 7680 caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc 7740 cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca 7800 actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc 7860 ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga 7920 tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga 7980 tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact 8040 gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt 8100 aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca 8160 aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac 8220 catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat 8280 ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc 8340 ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat 8400 caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca 8460 gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac 8520 cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct 8580 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt 8640 agcgcgaatt gatctg 8656 <210> 93 <211> 9789 <212> DNA <213> Artificial Sequence <220> <223> pSNAK(-E)-dxs <400> 93 gatctcatta ggtagccgtt tcaggcacaa ttgggattga taaaattagt ttttttgtgc 60 ccccttatta tattgatatg acggcactgg ctgaagccag aaatgtagac cctggaaaat 120 ttcatattgg tattgggcaa gaccaaatgg cggtgaaccc aatcagccaa gatattgtga 180 catttgcagc caatgccgca gaagcgatct tgaccaaaga agataaagag gccattgata 240 tggtgattgt cgggactgag tccagtatcg atgagtcaaa agcggccgca gttgtcttac 300 atcgtttaat ggggattcaa cctttcgctc gctctttcga aatcaaggaa gcttgttacg 360 gagcaacagc aggcttacag ttagctaaga atcacgtagc cttacatcca gataaaaaag 420 tcttggttgt agcagcagat attgcaaaat atggattaaa ttctggcggt gagcctacac 480 aaggagctgg ggcggttgca atgttagttg ctagtgaacc gcgcatcttg gctttaaaag 540 aggataatgt gatgctgacg caagatatct atgacttttg gcgtccaaca ggccatccgt 600 atcctatggt cgatggtcct ttgtcaaacg aaacctacat ccaatctttt gcccaagtct 660 gggatgaaca taaaaaaaga accggtcttg attttgcaga ttatgatgct ttagcgttcc 720 atattcctta cacaaaaatg ggcaaaaaag ccttattagc aaaaatctcc gaccaaactg 780 aagcagaaca ggaacgaatt ttagcccgtt atgaagaaag catcatctat agtcgtcgcg 840 taggaaactt gtatacgggt tcactttatc tgggactcat ttccctttta gaaaatgcaa 900 cgactttaac cgcaggcaat caaattgggt tattcagtta tggttctggt gctgtcgctg 960 aatttttcac tggtgaatta gtagctggtt atcaaaatca tttacaaaaa gaaactcatt 1020 tagcactgct agataatcgg acagaacttt ctatcgctga atatgaagcc atgtttgcag 1080 aaactttaga cacagatatt gatcaaacgt tagaagatga attaaaatat agtatttctg 1140 ctattaataa taccgttcgc tcttatcgaa actaactgca gcctcgacct gcaggcatgc 1200 aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca 1260 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg 1320 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgagctt atcgatgata 1380 agctgtcaaa catgagaatt acaacttata tcgtatgggg ctgacttcag gtgctacatt 1440 tgaagagata aattgcactg aaatctagaa atattttatc tgattaataa gatgatcttc 1500 ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa aacgaaaaaa ccgccttgca 1560 gggcggtttt tcgaaggttc tctgagctac caactctttg aaccgaggta actggcttgg 1620 aggagcgcag tcaccaaaac ttgtcctttc agtttagcct taaccggcgc atgacttcaa 1680 gactaactcc tctaaatcaa ttaccagtgg ctgctgccag tggtgctttt gcatgtcttt 1740 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcggac tgaacggggg 1800 gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg 1860 gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca 1920 ggagagcgca cgagggagcc gccaggggaa acgcctggta tctttatagt cctgtcgggt 1980 ttcgccacca ctgatttgag cgtcagattt cgtgatgctt gtcagggggg cggagcctat 2040 ggaaaaacgg ctttgccgcg gccctctcac ttccctgtta agtatcttcc tggcatcttc 2100 caggaaatct ccgccccgtt cgtaagccat ttccgctcgc cgcagtcgaa cgaccgagcg 2160 tagcgagtca gtgagcgagg aagcggaata tatcctgtat cacatattct gctgacgcac 2220 cggtgcagcc ttttttctcc tgccacatga agcacttcac tgacaccctc atcagtgcca 2280 acatagtaag ccagtataca ctccgctagc gctgatgtcc ggcggtgctt ttgccgttac 2340 gcaccacccc gtcagtagct gaacaggagg gacagctgat agaaacagaa gccactggag 2400 cacctcaaaa acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt 2460 tgcgccgaat aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc 2520 cctgttgata ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac 2580 gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag 2640 ttatcgagat tttcaggagc taaggaagct aaaatgagcc atattcaacg ggaaacgtct 2700 tgctctaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct 2760 cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg 2820 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg 2880 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt 2940 actcctgatg atgcatggtt actcaccact gcgatccccg ggaaaacagc attccaggta 3000 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc 3060 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc 3120 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag 3180 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca 3240 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg 3300 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt 3360 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa 3420 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag 3480 tttttctaaa gtactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt 3540 ggtgccctta aacgcctggt gctacgcctg aataagtgat aataagcgga tgaatggcag 3600 aaattcgaaa gcaaattcga cccggtcgtc ggttcagggc agggtcgtta aatagccgct 3660 tatgtctatt gctggtttac cggtttattg actaccggaa gcagtgtgac cgtgtgcttc 3720 tcaaatgcct gaggccagtt tgctcaggct ctccccgtgg aggtaataat tgacgatatg 3780 atcatttatt ctgcctccca gagcctgata aaaacggtta gcgcttcgtt aatacagatg 3840 taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc 3900 agggcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg 3960 ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga 4020 tagtcatgcc ccgcgcccac cggaaggagc taccggacag cggtgcggac tgttgtaact 4080 cagaataaga aatgaggccg ctcatggcgt tccaatacgc aaaccgcctc tccccgcgcg 4140 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga 4200 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat 4260 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag 4320 ctatgaccat gattacgaat tcgagctcgg tacccgggga tcctacctga cgctttttat 4380 cgcaactctc tactgtttct ccatacccgt ttttttgggc tagcaggagg aattcaccat 4440 ggtaccaatg acaaaaaaag ttggtgtcgg tcaggcacat agtaagataa ttttaatagg 4500 ggaacatgcg gtcgtttacg gttatcctgc catttccctg cctcttttgg aggtggaggt 4560 gacctgtaag gtagttcctg cagagagtcc ttggcgcctt tatgaggagg ataccttgtc 4620 catggcggtt tatgcctcac tggagtattt gaatatcaca gaagcctgca ttcgttgtga 4680 gattgactcg gctatccctg agaaacgggg gatgggttcg tcagcggcta tcagcatagc 4740 ggccattcgt gcagtatttg actactatca ggctgatctg cctcatgatg tactagaaat 4800 cttggtcaat cgagctgaaa tgattgccca tatgaatcct agtggtttgg atgctaagac 4860 ctgtcttagt gaccaaccta ttcgctttat caagaacgta ggatttacag aacttgagat 4920 ggatttatcc gcctatttgg tgattgccga tacgggtgtt tatggtcata ctcgtgaagc 4980 catccaagtg gttcaaaata agggcaagga tgccctaccg tttttgcatg ccttgggaga 5040 attaacccag caagcagaag ttgcgatttc acaaaaagat gctgaaggac tgggacaaat 5100 cctcagtcaa gcgcatttac atttaaaaga aattggagtc agtagccctg aggcagactt 5160 tttggttgaa acgactctta gccatggtgc tctgggtgcc aagatgagcg gtggtgggct 5220 aggaggttgt atcatagcct tggtaaccaa tttgacacac gcacaagaac tagcagaaag 5280 attagaagag aaaggagctg ttcagacatg gatagagagc ctgtaacagt acgttcctac 5340 gcaaatattg ctattatcaa atattgggga aagaaaaaag aaaaagagat ggtgcctgct 5400 actagcagta tttctctaac tttggaaaat atgtatacag agacgacctt gtcgccttta 5460 ccagccaatg taacagctga cgaattttac atcaatggtc agctacaaaa tgaggtcgag 5520 catgccaaga tgagtaagat tattgaccgt tatcgtccag ctggtgaggg ctttgtccgt 5580 atcgatactc aaaacaatat gcctacggca gcgggcctgt cctcaagttc tagtggtttg 5640 tccgccctgg tcaaggcttg taatgcttat ttcaagcttg gattggatag aagtcagttg 5700 gcacaggaag ccaaatttgc ctcaggctct tcttctcgga gtttttatgg accactagga 5760 gcctgggata aggatagtgg agaaatttac cctgtagaga cagacttgaa actagctatg 5820 attatgttgg tgctagagga caagaaaaaa ccaatctcta gccgtgacgg gatgaaactt 5880 tgtgtggaaa cctcgacgac ttttgacgac tgggttcgtc agtctgagaa ggactatcag 5940 gatatgctga tttatctcaa ggaaaatgat tttgccaaga ttggagaatt aacggagaaa 6000 aatgctctgg ctatgcatgc tacgacaaag actgctagtc cagccttttc ttatctgacg 6060 gatgcctctt atgaggctat ggcctttgtt cgccagcttc gtgagaaagg agaggcctgc 6120 tactttacca tggatgctgg tcccaatgtt aaggtcttct gtcaggagaa agacttggag 6180 catttgtcag aaattttcgg tcagcgttat cgcttgattg tgtcaaaaac aaaggatttg 6240 agtcaagatg attgctgtta aaacttgcgg aaaactctat tgggcaggtg aatatgctat 6300 tttagagcca gggcagttag ctttgataaa ggatattccc atctatatga gggctgagat 6360 tgctttttct gacagctacc gtatctattc agatatgttt gatttcgcag tggacttaag 6420 gcccaatcct gactacagct tgattcaaga aacgattgct ttgatgggag acttcctcgc 6480 tgttcgcggt cagaatttaa gacctttttc cctaaaaatc tgtggcaaaa tggaacgaga 6540 agggaaaaag tttggtctag gttctagtgg cagcgtcgtt gtcttggttg tcaaggcttt 6600 actggctctc tataatcttt cggttgatca gaatctcttg ttcaagctga ctagcgctgt 6660 cttgctcaag cgaggagaca atggttccat gggcgacctt gcctgtattg tggcagagga 6720 tttggttctt taccagtcat ttgatcgcca gaaggcggct gcttggttag aagaagaaaa 6780 cttggcgaca gttctggagc gtgattgggg attttttatc tcacaagtga aaccaacttt 6840 agaatgtgat ttcttagtgg gatggaccaa ggaagtggct gtatcgagtc acatggtcca 6900 gcaaatcaag caaaatatca atcaaaattt tttaagttcc tcaaaagaaa cggtggtttc 6960 tttggtcgaa gccttggagc aggggaaagc cgaaaaagtt atcgagcaag tagaagtagc 7020 cagcaagctt ttagaaggct tgagtacaga tatttacacg cctttgctta gacagttgaa 7080 agaagccagt caagatttgc aggccgttgc caagagtagt ggtgctggtg gtggtgactg 7140 tggcatcgcc ctgagttttg atgcgcaatc ttctcgaaac actttaaaaa atcgttgggc 7200 cgatctgggg attgagctct tatatcaaga aaggatagga catgacgaca aatcgtaatc 7260 tagccccggg aggagagaaa ttatgcaaac ggaacacgtc attttattga atgcacaggg 7320 agttcccacg ggtacgctgg aaaagtatgc cgcacacacg gcagacaccc gcttacatct 7380 cgcgttctcc agttggctgt ttaatgccaa aggacaatta ttagttaccc gccgcgcact 7440 gagcaaaaaa gcatggcctg gcgtgtggac taactcggtt tgtgggcacc cacaactggg 7500 agaaagcaac gaagacgcag tgatccgccg ttgccgttat gagcttggcg tggaaattac 7560 gcctcctgaa tctatctatc ctgactttcg ctaccgcgcc accgatccga gtggcattgt 7620 ggaaaatgaa gtgtgtccgg tatttgccgc acgcaccact agtgcgttac agatcaatga 7680 tgatgaagtg atggattatc aatggtgtga tttagcagat gtattacacg gtattgatgc 7740 cacgccgtgg gcgttcagtc cgtggatggt gatgcaggcg acaaatcgcg aagccagaaa 7800 acgattatct gcatttaccc agcttaaata agcatgcact agagtcgagg aaacagacca 7860 tggagtctcg agtgtggaat tgtgagcgga taacaatttc acacaggaaa cagaccatgg 7920 aattcatgag ttttgatatt gccaaatacc cgaccctggc actggtcgac tccacccagg 7980 agttacgact gttgccgaaa gagagtttac cgaaactctg cgacgaactg cgccgctatt 8040 tactcgacag cgtgagccgt tccagcgggc acttcgcctc cgggctgggc acggtcgaac 8100 tgaccgtggc gctgcactat gtctacaaca ccccgtttga ccaattgatt tgggatgtgg 8160 ggcatcaggc ttatccgcat aaaattttga ccggacgccg cgacaaaatc ggcaccatcc 8220 gtcagaaagg cggtctgcac ccgttcccgt ggcgcggcga aagcgaatat gacgtattaa 8280 gcgtcgggca ttcatcaacc tccatcagtg ccggaattgg tattgcggtt gctgccgaaa 8340 aagaaggcaa aaatcgccgc accgtctgtg tcattggcga tggcgcgatt accgcaggca 8400 tggcgtttga agcgatgaat cacgcgggcg atatccgtcc tgatatgctg gtgattctca 8460 acgacaatga aatgtcgatt tccgaaaatg tcggcgcgct caacaaccat ctggcacagc 8520 tgctttccgg taagctttac tcttcactgc gcgaaggcgg gaaaaaagtt ttctctggcg 8580 tgccgccaat taaagagctg ctcaaacgca ccgaagaaca tattaaaggc atggtagtgc 8640 ctggcacgtt gtttgaagag ctgggcttta actacatcgg cccggtggac ggtcacgatg 8700 tgctggggct tatcaccacg ctaaagaaca tgcgcgacct gaaaggcccg cagttcctgc 8760 atatcatgac caaaaaaggt cgtggttatg aaccggcaga aaaagacccg atcactttcc 8820 acgccgtgcc taaatttgat ccctccagcg gttgtttgcc gaaaagtagc ggcggtttgc 8880 cgagctattc aaaaatcttt ggcgactggt tgtgcgaaac ggcagcgaaa gacaacaagc 8940 tgatggcgat tactccggcg atgcgtgaag gttccggcat ggtcgagttt tcacgtaaat 9000 tcccggatcg ctacttcgac gtggcaattg ccgagcaaca cgcggtgacc tttgctgcgg 9060 gtctggcgat tggtgggtac aaacccattg tcgcgattta ctccactttc ctgcaacgcg 9120 cctatgatca ggtgctgcat gacgtggcga ttcaaaagct tccggtcctg ttcgccatcg 9180 accgcgcggg cattgttggt gctgacggtc aaacccatca gggtgctttt gatctctctt 9240 acctgcgctg cataccggaa atggtcatta tgaccccgag cgatgaaaac gaatgtcgcc 9300 agatgctcta taccggctat cactataacg atggcccgtc agcggtgcgc tacccgcgtg 9360 gcaacgcggt cggcgtggaa ctgacgccgc tggaaaaact accaattggc aaaggcattg 9420 tgaagcgtcg tggcgagaaa ctggcgatcc ttaactttgg tacgctgatg ccagaagcgg 9480 cgaaagtcgc cgaatcgctg aacgccacgc tggtcgatat gcgttttgtg aaaccgcttg 9540 atgaagcgtt aattctggaa atggccgcca gccatgaagc gctggtcacc gtagaagaaa 9600 acgccattat gggcggcgca ggcagcggcg tgaacgaagt gctgatggcc catcgtaaac 9660 cagtacccgt gctgaacatt ggcctgccgg acttctttat tccgcaagga actcaggaag 9720 aaatgcgcgc cgaactcggc ctcgatgccg ctggtatgga agccaaaatc aaggcctggc 9780 tggcataaa 9789 <210> 94 <211> 8938 <212> DNA <213> Artificial Sequence <220> <223> pSNA(-E)-dxsfree <400> 94 gatctcatta ggtagccgtt tcaggcacaa ttgggattga taaaattagt ttttttgtgc 60 ccccttatta tattgatatg acggcactgg ctgaagccag aaatgtagac cctggaaaat 120 ttcatattgg tattgggcaa gaccaaatgg cggtgaaccc aatcagccaa gatattgtga 180 catttgcagc caatgccgca gaagcgatct tgaccaaaga agataaagag gccattgata 240 tggtgattgt cgggactgag tccagtatcg atgagtcaaa agcggccgca gttgtcttac 300 atcgtttaat ggggattcaa cctttcgctc gctctttcga aatcaaggaa gcttgttacg 360 gagcaacagc aggcttacag ttagctaaga atcacgtagc cttacatcca gataaaaaag 420 tcttggttgt agcagcagat attgcaaaat atggattaaa ttctggcggt gagcctacac 480 aaggagctgg ggcggttgca atgttagttg ctagtgaacc gcgcatcttg gctttaaaag 540 aggataatgt gatgctgacg caagatatct atgacttttg gcgtccaaca ggccatccgt 600 atcctatggt cgatggtcct ttgtcaaacg aaacctacat ccaatctttt gcccaagtct 660 gggatgaaca taaaaaaaga accggtcttg attttgcaga ttatgatgct ttagcgttcc 720 atattcctta cacaaaaatg ggcaaaaaag ccttattagc aaaaatctcc gaccaaactg 780 aagcagaaca ggaacgaatt ttagcccgtt atgaagaaag catcatctat agtcgtcgcg 840 taggaaactt gtatacgggt tcactttatc tgggactcat ttccctttta gaaaatgcaa 900 cgactttaac cgcaggcaat caaattgggt tattcagtta tggttctggt gctgtcgctg 960 aatttttcac tggtgaatta gtagctggtt atcaaaatca tttacaaaaa gaaactcatt 1020 tagcactgct agataatcgg acagaacttt ctatcgctga atatgaagcc atgtttgcag 1080 aaactttaga cacagatatt gatcaaacgt tagaagatga attaaaatat agtatttctg 1140 ctattaataa taccgttcgc tcttatcgaa actaactgca gcctcgacct gcaggcatgc 1200 aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca 1260 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg 1320 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgagctt atcgatgata 1380 agctgtcaaa catgagaatt acaacttata tcgtatgggg ctgacttcag gtgctacatt 1440 tgaagagata aattgcactg aaatctagaa atattttatc tgattaataa gatgatcttc 1500 ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa aacgaaaaaa ccgccttgca 1560 gggcggtttt tcgaaggttc tctgagctac caactctttg aaccgaggta actggcttgg 1620 aggagcgcag tcaccaaaac ttgtcctttc agtttagcct taaccggcgc atgacttcaa 1680 gactaactcc tctaaatcaa ttaccagtgg ctgctgccag tggtgctttt gcatgtcttt 1740 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcggac tgaacggggg 1800 gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg 1860 gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca 1920 ggagagcgca cgagggagcc gccaggggaa acgcctggta tctttatagt cctgtcgggt 1980 ttcgccacca ctgatttgag cgtcagattt cgtgatgctt gtcagggggg cggagcctat 2040 ggaaaaacgg ctttgccgcg gccctctcac ttccctgtta agtatcttcc tggcatcttc 2100 caggaaatct ccgccccgtt cgtaagccat ttccgctcgc cgcagtcgaa cgaccgagcg 2160 tagcgagtca gtgagcgagg aagcggaata tatcctgtat cacatattct gctgacgcac 2220 cggtgcagcc ttttttctcc tgccacatga agcacttcac tgacaccctc atcagtgcca 2280 acatagtaag ccagtataca ctccgctagc gctgatgtcc ggcggtgctt ttgccgttac 2340 gcaccacccc gtcagtagct gaacaggagg gacagctgat agaaacagaa gccactggag 2400 cacctcaaaa acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt 2460 tgcgccgaat aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc 2520 cctgttgata ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac 2580 gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag 2640 ttatcgagat tttcaggagc taaggaagct tttttttaag gcagttattg gtgcccttaa 2700 acgcctggtg ctacgcctga ataagtgata ataagcggat gaatggcaga aattcgaaag 2760 caaattcgac ccggtcgtcg gttcagggca gggtcgttaa atagccgctt atgtctattg 2820 ctggtttacc ggtttattga ctaccggaag cagtgtgacc gtgtgcttct caaatgcctg 2880 aggccagttt gctcaggctc tccccgtgga ggtaataatt gacgatatga tcatttattc 2940 tgcctcccag agcctgataa aaacggttag cgcttcgtta atacagatgt aggtgttcca 3000 cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgcttgt 3060 ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc tgccggcacc 3120 tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc 3180 cgcgcccacc ggaaggagct accggacagc ggtgcggact gttgtaactc agaataagaa 3240 atgaggccgc tcatggcgtt ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 3300 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 3360 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc 3420 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg 3480 attacgaatt cgagctcggt acccggggat cctacctgac gctttttatc gcaactctct 3540 actgtttctc catacccgtt tttttgggct agcaggagga attcaccatg gtaccaatga 3600 caaaaaaagt tggtgtcggt caggcacata gtaagataat tttaataggg gaacatgcgg 3660 tcgtttacgg ttatcctgcc atttccctgc ctcttttgga ggtggaggtg acctgtaagg 3720 tagttcctgc agagagtcct tggcgccttt atgaggagga taccttgtcc atggcggttt 3780 atgcctcact ggagtatttg aatatcacag aagcctgcat tcgttgtgag attgactcgg 3840 ctatccctga gaaacggggg atgggttcgt cagcggctat cagcatagcg gccattcgtg 3900 cagtatttga ctactatcag gctgatctgc ctcatgatgt actagaaatc ttggtcaatc 3960 gagctgaaat gattgcccat atgaatccta gtggtttgga tgctaagacc tgtcttagtg 4020 accaacctat tcgctttatc aagaacgtag gatttacaga acttgagatg gatttatccg 4080 cctatttggt gattgccgat acgggtgttt atggtcatac tcgtgaagcc atccaagtgg 4140 ttcaaaataa gggcaaggat gccctaccgt ttttgcatgc cttgggagaa ttaacccagc 4200 aagcagaagt tgcgatttca caaaaagatg ctgaaggact gggacaaatc ctcagtcaag 4260 cgcatttaca tttaaaagaa attggagtca gtagccctga ggcagacttt ttggttgaaa 4320 cgactcttag ccatggtgct ctgggtgcca agatgagcgg tggtgggcta ggaggttgta 4380 tcatagcctt ggtaaccaat ttgacacacg cacaagaact agcagaaaga ttagaagaga 4440 aaggagctgt tcagacatgg atagagagcc tgtaacagta cgttcctacg caaatattgc 4500 tattatcaaa tattggggaa agaaaaaaga aaaagagatg gtgcctgcta ctagcagtat 4560 ttctctaact ttggaaaata tgtatacaga gacgaccttg tcgcctttac cagccaatgt 4620 aacagctgac gaattttaca tcaatggtca gctacaaaat gaggtcgagc atgccaagat 4680 gagtaagatt attgaccgtt atcgtccagc tggtgagggc tttgtccgta tcgatactca 4740 aaacaatatg cctacggcag cgggcctgtc ctcaagttct agtggtttgt ccgccctggt 4800 caaggcttgt aatgcttatt tcaagcttgg attggataga agtcagttgg cacaggaagc 4860 caaatttgcc tcaggctctt cttctcggag tttttatgga ccactaggag cctgggataa 4920 ggatagtgga gaaatttacc ctgtagagac agacttgaaa ctagctatga ttatgttggt 4980 gctagaggac aagaaaaaac caatctctag ccgtgacggg atgaaacttt gtgtggaaac 5040 ctcgacgact tttgacgact gggttcgtca gtctgagaag gactatcagg atatgctgat 5100 ttatctcaag gaaaatgatt ttgccaagat tggagaatta acggagaaaa atgctctggc 5160 tatgcatgct acgacaaaga ctgctagtcc agccttttct tatctgacgg atgcctctta 5220 tgaggctatg gcctttgttc gccagcttcg tgagaaagga gaggcctgct actttaccat 5280 ggatgctggt cccaatgtta aggtcttctg tcaggagaaa gacttggagc atttgtcaga 5340 aattttcggt cagcgttatc gcttgattgt gtcaaaaaca aaggatttga gtcaagatga 5400 ttgctgttaa aacttgcgga aaactctatt gggcaggtga atatgctatt ttagagccag 5460 ggcagttagc tttgataaag gatattccca tctatatgag ggctgagatt gctttttctg 5520 acagctaccg tatctattca gatatgtttg atttcgcagt ggacttaagg cccaatcctg 5580 actacagctt gattcaagaa acgattgctt tgatgggaga cttcctcgct gttcgcggtc 5640 agaatttaag acctttttcc ctaaaaatct gtggcaaaat ggaacgagaa gggaaaaagt 5700 ttggtctagg ttctagtggc agcgtcgttg tcttggttgt caaggcttta ctggctctct 5760 ataatctttc ggttgatcag aatctcttgt tcaagctgac tagcgctgtc ttgctcaagc 5820 gaggagacaa tggttccatg ggcgaccttg cctgtattgt ggcagaggat ttggttcttt 5880 accagtcatt tgatcgccag aaggcggctg cttggttaga agaagaaaac ttggcgacag 5940 ttctggagcg tgattgggga ttttttatct cacaagtgaa accaacttta gaatgtgatt 6000 tcttagtggg atggaccaag gaagtggctg tatcgagtca catggtccag caaatcaagc 6060 aaaatatcaa tcaaaatttt ttaagttcct caaaagaaac ggtggtttct ttggtcgaag 6120 ccttggagca ggggaaagcc gaaaaagtta tcgagcaagt agaagtagcc agcaagcttt 6180 tagaaggctt gagtacagat atttacacgc ctttgcttag acagttgaaa gaagccagtc 6240 aagatttgca ggccgttgcc aagagtagtg gtgctggtgg tggtgactgt ggcatcgccc 6300 tgagttttga tgcgcaatct tctcgaaaca ctttaaaaaa tcgttgggcc gatctgggga 6360 ttgagctctt atatcaagaa aggataggac atgacgacaa atcgtaatct agccccggga 6420 ggagagaaat tatgcaaacg gaacacgtca ttttattgaa tgcacaggga gttcccacgg 6480 gtacgctgga aaagtatgcc gcacacacgg cagacacccg cttacatctc gcgttctcca 6540 gttggctgtt taatgccaaa ggacaattat tagttacccg ccgcgcactg agcaaaaaag 6600 catggcctgg cgtgtggact aactcggttt gtgggcaccc acaactggga gaaagcaacg 6660 aagacgcagt gatccgccgt tgccgttatg agcttggcgt ggaaattacg cctcctgaat 6720 ctatctatcc tgactttcgc taccgcgcca ccgatccgag tggcattgtg gaaaatgaag 6780 tgtgtccggt atttgccgca cgcaccacta gtgcgttaca gatcaatgat gatgaagtga 6840 tggattatca atggtgtgat ttagcagatg tattacacgg tattgatgcc acgccgtggg 6900 cgttcagtcc gtggatggtg atgcaggcga caaatcgcga agccagaaaa cgattatctg 6960 catttaccca gcttaaataa gcatgcacta gagtcgagga aacagaccat ggagtctcga 7020 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agaccatgga attcatgagt 7080 tttgatattg ccaaataccc gaccctggca ctggtcgact ccacccagga gttacgactg 7140 ttgccgaaag agagtttacc gaaactctgc gacgaactgc gccgctattt actcgacagc 7200 gtgagccgtt ccagcgggca cttcgcctcc gggctgggca cggtcgaact gaccgtggcg 7260 ctgcactatg tctacaacac cccgtttgac caattgattt gggatgtggg gcatcaggct 7320 tatccgcata aaattttgac cggacgccgc gacaaaatcg gcaccatccg tcagaaaggc 7380 ggtctgcacc cgttcccgtg gcgcggcgaa agcgaatatg acgtattaag cgtcgggcat 7440 tcatcaacct ccatcagtgc cggaattggt attgcggttg ctgccgaaaa agaaggcaaa 7500 aatcgccgca ccgtctgtgt cattggcgat ggcgcgatta ccgcaggcat ggcgtttgaa 7560 gcgatgaatc acgcgggcga tatccgtcct gatatgctgg tgattctcaa cgacaatgaa 7620 atgtcgattt ccgaaaatgt cggcgcgctc aacaaccatc tggcacagct gctttccggt 7680 aagctttact cttcactgcg cgaaggcggg aaaaaagttt tctctggcgt gccgccaatt 7740 aaagagctgc tcaaacgcac cgaagaacat attaaaggca tggtagtgcc tggcacgttg 7800 tttgaagagc tgggctttaa ctacatcggc ccggtggacg gtcacgatgt gctggggctt 7860 atcaccacgc taaagaacat gcgcgacctg aaaggcccgc agttcctgca tatcatgacc 7920 aaaaaaggtc gtggttatga accggcagaa aaagacccga tcactttcca cgccgtgcct 7980 aaatttgatc cctccagcgg ttgtttgccg aaaagtagcg gcggtttgcc gagctattca 8040 aaaatctttg gcgactggtt gtgcgaaacg gcagcgaaag acaacaagct gatggcgatt 8100 actccggcga tgcgtgaagg ttccggcatg gtcgagtttt cacgtaaatt cccggatcgc 8160 tacttcgacg tggcaattgc cgagcaacac gcggtgacct ttgctgcggg tctggcgatt 8220 ggtgggtaca aacccattgt cgcgatttac tccactttcc tgcaacgcgc ctatgatcag 8280 gtgctgcatg acgtggcgat tcaaaagctt ccggtcctgt tcgccatcga ccgcgcgggc 8340 attgttggtg ctgacggtca aacccatcag ggtgcttttg atctctctta cctgcgctgc 8400 ataccggaaa tggtcattat gaccccgagc gatgaaaacg aatgtcgcca gatgctctat 8460 accggctatc actataacga tggcccgtca gcggtgcgct acccgcgtgg caacgcggtc 8520 ggcgtggaac tgacgccgct ggaaaaacta ccaattggca aaggcattgt gaagcgtcgt 8580 ggcgagaaac tggcgatcct taactttggt acgctgatgc cagaagcggc gaaagtcgcc 8640 gaatcgctga acgccacgct ggtcgatatg cgttttgtga aaccgcttga tgaagcgtta 8700 attctggaaa tggccgccag ccatgaagcg ctggtcaccg tagaagaaaa cgccattatg 8760 ggcggcgcag gcagcggcgt gaacgaagtg ctgatggccc atcgtaaacc agtacccgtg 8820 ctgaacattg gcctgccgga cttctttatt ccgcaaggaa ctcaggaaga aatgcgcgcc 8880 gaactcggcc tcgatgccgc tggtatggaa gccaaaatca aggcctggct ggcataaa 8938 <210> 95 <211> 14034 <212> DNA <213> Artificial Sequence <220> <223> pT-HBSREYbbOdxrfree <400> 95 ctagcacaga atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac 60 tcagaagtga aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg 120 aactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 180 ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa 240 cgttgcgaag caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca 300 tcaaattaag cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactctttt 360 tgtttatttt tctaaataca ttcaaatatg tatccgctca aagcttgcgt cagaccccgt 420 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 480 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 540 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta 600 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 660 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 720 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 780 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 840 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 900 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 960 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 1020 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 1080 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt 1140 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga 1200 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca 1260 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac 1320 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct 1380 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc 1440 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagcag 1500 atcaattcgc gcgcgaaggc gaagcggcat gcatttacgt tgacaccatc gaatggtgca 1560 aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg gtggtgaatg 1620 tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt 1680 cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg 1740 cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt 1800 cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg 1860 cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac 1920 gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg 1980 ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca 2040 ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt 2100 tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc 2160 aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct 2220 ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga 2280 gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg 2340 cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg 2400 ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat 2460 gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg 2520 tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg 2580 tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg 2640 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt 2700 gagcgcaacg caattaatgt gagttagcgc gaattgatct ggtttgacag cttatcatcg 2760 actgcacggt gcaccaatgc ttctggcgtc aggcagccat cggaagctgt ggtatggctg 2820 tgcaggtcgt aaatcactgc ataattcgtg tcgctcaagg cgcactcccg ttctggataa 2880 tgttttttgc gccgacatca taacggttct ggcaaatatt ctgaaatgag ctgttgacaa 2940 ttaatcatcc ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt cacacaggaa 3000 acagaccatg gaattctacg tactagtgaa ttcaggaggt aataaatatg ggtctgatgc 3060 tgattgattg gtgtgcactg gctctggttg ttttcattgg cctgccgcac ggcgcgctgg 3120 atgctgccat ttctttttct atgatctctt ctgcaaaacg cattgctcgt ctggctggta 3180 ttctgctgat ctatctgctg ctggcgaccg cgttcttcct gatctggtat cagctgccag 3240 cgtttagcct gctgatcttc ctgctgatct ccattatcca ctttggtatg gcagacttca 3300 acgcgtcccc aagcaaactg aaatggccgc atatcatcgc ccacggcggt gttgttactg 3360 tttggctgcc gctgatccag aaaaacgaag taactaaact gtttagcatc ctgactaacg 3420 gtccgactcc gatcctgtgg gacatcctgc tgattttctt cctgtgttgg tctattggcg 3480 tgtgtctgca cacgtacgaa accctgcgct ctaaacatta caacatcgcc tttgaactga 3540 tcggtctgat tttcctggcg tggtatgcgc cgcctctggt tacgtttgcc acttacttct 3600 gcttcattca ttcccgtcgc cacttctcct ttgtgtggaa gcagctgcaa cacatgtctt 3660 ccaaaaagat gatgattggc agcgcgatta tcctgtcctg tacctcttgg ctgatcggcg 3720 gtggtatcta tttcttcctg aactccaaaa tgatcgcctc tgaggctgcg ctgcagactg 3780 tgttcatcgg tctggcggca ctgaccgtgc cgcacatgat tctgatcgac ttcatcttcc 3840 gtccgcactc ttcccgtatc aaaatcaaaa actaattaat taaaggaggt aataatatga 3900 ctcataaagc aacggagatc ctgacaggta aagttatgca aaaatcggtc ttaattaccg 3960 gatgttccag tggaattggc ctggaaagcg cgctcgaatt aaaacgccag ggttttcatg 4020 tgctggcagg ttgccggaaa ccggatgatg ttgagcgcat gaacagcatg ggatttaccg 4080 gcgtgttgat cgatctggat tcaccagaaa gtgttgatcg cgcagccgac gaggtgatcg 4140 ccctgaccga taattgtctg tatgggatct ttaacaatgc cggattcggc atgtatggcc 4200 ccctttccac catcagccgt gcgcagatgg aacagcagtt ttccgccaac tttttcggcg 4260 cacaccagct caccatgcgc ctgttacccg cgatgttacc gcacggtgaa gggcgtattg 4320 tgatgacatc atcggtgatg ggattaatct ccacgccggg tcgtggcgct tacgcggcca 4380 gtaaatatgc gctggaggcg tggtcagatg cactgcgcat ggagctgcgc cacagcggaa 4440 ttaaagtcag cctgatcgaa cccggtccca ttcgtactcg cttcaccgac aacgtcaacc 4500 agacgcaaag tgataaacca gtcgaaaatc ccggcatcgc cgcccgcttt acgttgggac 4560 cggaagcggt ggtggacaaa gtacgccatg cttttattag cgagaagccg aagatgcgct 4620 atccggtgac gctggtgacc tgggcggtaa tggtgcttaa gcgcctgctg ccggggcgcg 4680 tgatggacaa aatattgcag gggtaactag taggaggtaa taaatatggt gagtggcagt 4740 aaagcgggcg tttcgcctca tcgcgaaata gaagtaatga gacaatccat tgacgatcac 4800 ctggctggcc tgttacctga aaccgacagc caggatatcg tcagccttgc gatgcgtgaa 4860 ggcgtcatgg cacccggtaa acggatccgt ccgctgctga tgctgctggc cgcccgcgac 4920 ctccgctacc agggcagtat gcctacgctg ctcgatctcg cctgcgccgt tgaactgacc 4980 cataccgcgt cgctgatgct cgacgacatg ccctgcatgg acaacgccga gctgcgccgc 5040 ggtcagccca ctacccacaa aaaatttggt gagagcgtgg cgatccttgc ctccgttggg 5100 ctgctctcta aagcctttgg tctgatcgcc gccaccggcg atctgccggg ggagaggcgt 5160 gcccaggcgg tcaacgagct ctctaccgcc gtgggcgtgc agggcctggt actggggcag 5220 tttcgcgatc ttaacgatgc cgccctcgac cgtacccctg acgctatcct cagcaccaac 5280 cacctcaaga ccggcattct gttcagcgcg atgctgcaga tcgtcgccat tgcttccgcc 5340 tcgtcgccga gcacgcgaga gacgctgcac gccttcgccc tcgacttcgg ccaggcgttt 5400 caactgctgg acgatctgcg tgacgatcac ccggaaaccg gtaaagatcg caataaggac 5460 gcgggaaaat cgacgctggt caaccggctg ggcgcagacg cggcccggca aaagctgcgc 5520 gagcatattg attccgccga caaacacctc acttttgcct gtccgcaggg cggcgccatc 5580 cgacagttta tgcatctgtg gtttggccat caccttgccg actggtcacc ggtcatgaaa 5640 atcgcctgat aactcgagga ggtataaagg atgaaaaaaa ccgttgtgat tggcgcaggc 5700 tttggtggcc tggcgctggc gattcgcctg caggcggcag ggatcccaac cgtactgctg 5760 gagcagcggg acaagcccgg cggtcgggcc tacgtctggc atgaccaggg ctttaccttt 5820 gacgccgggc cgacggtgat caccgatcct accgcgcttg aggcgctgtt caccctggcc 5880 ggcaggcgca tggaggatta cgtcaggctg ctgccggtaa aacccttcta ccgactctgc 5940 tgggagtccg ggaagaccct cgactatgct aacgacagcg ccgagcttga ggcgcagatt 6000 acccagttca acccccgcga cgtcgagggc taccggcgct ttctggctta ctcccaggcg 6060 gtattccagg agggatattt gcgcctcggc agcgtgccgt tcctctcttt tcgcgacatg 6120 ctgcgcgccg ggccgcagct gcttaagctc caggcgtggc agagcgtcta ccagtcggtt 6180 tcgcgcttta ttgaggatga gcatctgcgg caggccttct cgttccactc cctgctggta 6240 ggcggcaacc ccttcaccac ctcgtccatc tacaccctga tccacgccct tgagcgggag 6300 tggggggtct ggttccctga gggcggcacc ggggcgctgg tgaacggcat ggtgaagctg 6360 tttaccgatc tgggcgggga gatcgaactc aacgcccggg tcgaagagct ggtggtggcc 6420 gataaccgcg taagccaggt ccggctggcg gatggtcgga tctttgacac cgacgccgta 6480 gcctcgaacg ctgacgtggt gaacacctat aaaaagctgc tcggccacca tccggtgggg 6540 cagaagcggg cggcagcgct ggagcgcaag agcatgagca actcgctgtt tgtgctctac 6600 ttcggcctga accagcctca ttcccagctg gcgcaccata ccatctgttt tggtccccgc 6660 taccgggagc tgatcgacga gatctttacc ggcagcgcgc tggcggatga cttctcgctc 6720 tacctgcact cgccctgcgt gaccgatccc tcgctcgcgc ctcccggctg cgccagcttc 6780 tacgtgctgg ccccggtgcc gcatcttggc aacgcgccgc tggactgggc gcaggagggg 6840 ccgaagctgc gcgaccgcat ctttgactac cttgaagagc gctatatgcc cggcctgcgt 6900 agccagctgg tgacccagcg gatctttacc ccggcagact tccacgacac gctggatgcg 6960 catctgggat cggccttctc catcgagccg ctgctgaccc aaagcgcctg gttccgcccg 7020 cacaaccgcg acagcgacat tgccaacctc tacctggtgg gcgcaggtac tcaccctggg 7080 gcgggcattc ctggcgtagt ggcctcggcg aaagccaccg ccagcctgat gattgaggat 7140 ctgcaatgag ccaaccgccg ctgcttgacc acgccacgca gaccatggcc aacggctcga 7200 aaagttttgc caccgctgcg aagctgttcg acccggccac ccgccgtagc gtgctgatgc 7260 tctacacctg gtgccgccac tgcgatgacg tcattgacga ccagacccac ggcttcgcca 7320 gcgaggccgc ggcggaggag gaggccaccc agcgcctggc ccggctgcgc acgctgaccc 7380 tggcggcgtt tgaaggggcc gagatgcagg atccggcctt cgctgccttt caggaggtgg 7440 cgctgaccca cggtattacg ccccgcatgg cgctcgatca cctcgacggc tttgcgatgg 7500 acgtggctca gacccgctat gtcacctttg aggatacgct gcgctactgc tatcacgtgg 7560 cgggcgtggt gggtctgatg atggccaggg tgatgggcgt gcgggatgag cgggtgctgg 7620 atcgcgcctg cgatctgggg ctggccttcc agctgacgaa tatcgcccgg gatattattg 7680 acgatgcggc tattgaccgc tgctatctgc ccgccgagtg gctgcaggat gccgggctga 7740 ccccggagaa ctatgccgcg cgggagaatc gggccgcgct ggcgcgggtg gcggagcggc 7800 ttattgatgc cgcagagccg tactacatct cctcccaggc cgggctacac gatctgccgc 7860 cgcgctgcgc ctgggcgatc gccaccgccc gcagcgtcta ccgggagatc ggtattaagg 7920 taaaagcggc gggaggcagc gcctgggatc gccgccagca caccagcaaa ggtgaaaaaa 7980 ttgccatgct gatggcggca ccggggcagg ttattcgggc gaagacgacg agggtgacgc 8040 cgcgtccggc cggtctttgg cagcgtcccg tttagtaatc tagaggaggt aataaaatat 8100 gcttcgttcg ttgctcagag gcctcacgca tatcccccgc gtgaactccg cccagcagcc 8160 cagctgtgca cacgcgcgac tccagtttaa gctcaggagc atgcagatga cgctcatgca 8220 gcccagcatc tcagccaatc tgtcgcgcgc cgaggaccgc acagaccaca tgaggggtgc 8280 aagcacctgg gcaggcgggc agtcgcagga tgagctgatg ctgaaggacg agtgcatctt 8340 ggtggatgtt gaggacaaca tcacaggcca tgccagcaag ctggagtgtc acaagttcct 8400 accacatcag cctgcaggcc tgctgcaccg ggccttctct gtgttcctgt ttgacgatca 8460 ggggcgactg ctgctgcaac agcgtgcacg ctcaaaaatc accttcccaa gtgtgtggac 8520 gaacacctgc tgcagccacc ctttacatgg gcagacccca gatgaggtgg accaactaag 8580 ccaggtggcc gacggaacag tacctggcgc aaaggctgct gccatccgca agttggagca 8640 cgagctgggg ataccagcgc accagctgcc ggcaagcgcg tttcgcttcc tcacgcgttt 8700 gcactactgt gccgcggacg tgcagccagc tgcgacacaa tcagcgctct ggggcgagca 8760 cgaaatggac tacatcttgt tcatccgggc caacgtcacc ttggcgccca accctgacga 8820 ggtggacgaa gtcaggtacg tgacgcaaga ggagctgcgg cagatgatgc agccggacaa 8880 cgggctgcaa tggtcgccgt ggtttcgcat catcgccgcg cgcttccttg agcgttggtg 8940 ggctgacctg gacgcggccc taaacactga caaacacgag gattggggaa cggtgcatca 9000 catcaacgaa gcgtgataag cggccgcgct gttgacaatt aatcatccgg ctcgtataat 9060 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agaccatgga gttgaaaaca 9120 gtagttatta ttgatgcatt acgaacacca attggaaaat ataaaggcag cttaagtcaa 9180 gtaagtgccg tagacttagg aacacatgtt acaacacaac ttttaaaaag acattccact 9240 atttctgaag aaattgatca agtaatcttt ggaaatgttt tacaagctgg aaatggccaa 9300 aatcccgcac gacaaatagc aataaacagc ggtttatctc atgaaattcc cgcaatgaca 9360 gttaatgagg tctgcggatc aggaatgaag gccgttattt tggcgaaaca attgattcaa 9420 ttaggagaag cggaagtttt aattgctggc gggattgaga atatgtccca agcacctaaa 9480 ttacaacgat ttaattacga aacagaaagc tatgatgcgc ctttttctag tatgatgtac 9540 gatgggttaa cggatgcctt tagtggtcaa gcaatgggct taactgctga aaatgtggcc 9600 gaaaagtatc atgtaactag agaagagcaa gatcaatttt ctgtacattc acaattaaaa 9660 gcagctcaag cacaagcaga agggatattc gctgacgaaa tagccccatt agaagtatca 9720 ggaacgcttg tggagaaaga tgaagggatt cgccctaatt cgagcgttga gaagctagga 9780 acgcttaaaa cagtttttaa agaagacggt actgtaacag cagggaatgc atcaaccatt 9840 aatgatgggg cttctgcttt gattattgct tcacaagaat atgccgaagc acacggtctt 9900 ccttatttag ctattattcg agacagtgtg gaagtcggta ttgatccagc ctatatggga 9960 atttcgccga ttaaagccat tcaaaaactg ttagcgcgca atcaacttac tacggaagaa 10020 attgatctgt atgaaatcaa cgaagcattt gcagcaactt caatcgtggt ccaaagagaa 10080 ctggctttac cagaggaaaa ggtcaacatt tatggtggcg gtatttcatt aggtcatgcg 10140 attggtgcca caggtgctcg tttattaacg agtttaagtt atcaattaaa tcaaaaagaa 10200 aagaaatatg gagtggcttc tttatgtatc ggcggtggct taggactcgc tatgctacta 10260 gagagacctc agcaaaaaaa aaacagccga ttttatcaaa tgagtcctga ggaacgcctg 10320 gcttctcttc ttaatgaagg ccagatttct gctgatacaa aaaaagaatt tgaaaatacg 10380 gctttatctt cgcagattgc caatcatatg attgaaaatc aaatcagtga aacagaagtg 10440 ccgatgggcg ttggcttaca tttaacagtg gacgaaactg attatttggt accaatggcg 10500 acagaagagc cctcagtgat tgcggctttg agtaatggtg caaaaatagc acaaggattt 10560 aaaacagtga atcaacaacg tttaatgcgt ggacaaatcg ttttttacga tgttgcagac 10620 gccgagtcat tgattgatga actacaagta agagaaacgg aaatttttca acaagcagag 10680 ttaagttatc catctatcgt taaacgcggc ggcggcttaa gagatttgca atatcgtgct 10740 tttgatgaat catttgtatc tgtcgacttt ttagtagatg ttaaggatgc aatgggggca 10800 aatatcgtta acgctatgtt ggaaggtgtg gccgagttgt tccgtgaatg gtttgcggag 10860 caaaagattt tattcagtat tttaagtaat tatgccacgg agtcggttgt tacgatgaaa 10920 acggctattc cagtttcacg tttaagtaag gggagcaatg gccgggaaat tgctgaaaaa 10980 attgttttag cttcacgcta tgcttcatta gatccttatc gggcagtcac gcataacaaa 11040 gggatcatga atggcattga agctgtcgtt ttagctacag gaaatgatac acgcgctgtt 11100 agcgcttctt gtcatgcttt tgcggtgaag gaaggtcgct accaaggttt gactagttgg 11160 acgctggatg gcgaacaact aattggtgaa atttcagttc cgcttgcgtt agccacggtt 11220 ggcggtgcca caaaagtctt acctaaatct caagcagctg ctgatttgtt agcagtgacg 11280 gatgcaaaag aactaagtcg agtagtagcg gctgttggtt tggcacaaaa tttagcggcg 11340 ttacgggcct tagtctctga aggaattcaa aaaggacaca tggctctaca agcacgttct 11400 ttagcgatga cggtcggagc tactggtaaa gaagttgagg cagtcgctca acaattaaaa 11460 cgtcaaaaaa cgatgaacca agaccgagcc ttggctattt taaatgattt aagaaaacaa 11520 taaaaaaaca gttcagcaga aattattctg ctgaactgtt ttttttcaca ttaggtagcc 11580 gtttcagctc gacaggagga gcggctatgc aaccgcatta tgatctgatt ctcgtggggg 11640 ctggactcgc gaatggcctt atcgccctgc gtcttcagca gcagcaacct gatatgcgta 11700 ttttgcttat cgacgccgca ccccaggcgg gcgggaatca tacgtggtca tttcaccacg 11760 atgatttgac tgagagccaa catcgttgga tagctccgct ggtggttcat cactggcccg 11820 actatcaggt acgctttccc acacgccgtc gtaagctgaa cagcggctac ttttgtatta 11880 cttctcagcg tttcgctgag gttttacagc gacagtttgg cccgcacttg tggatggata 11940 ccgcggtcgc agaggttaat gcggaatctg ttcggttgaa aaagggtcag gttatcggtg 12000 cccgcgcggt gattgacggg cggggttatg cggcaaattc agcactgagc gtgggcttcc 12060 aggcgtttat tggccaggaa tggcgattga gccacccgca tggtttatcg tctcccatta 12120 tcatggatgc cacggtcgat cagcaaaatg gttatcgctt cgtgtacagc ctgccgctct 12180 cgccgaccag attgttaatt gaagacacgc actatattga taatgcgaca ttagatcctg 12240 aatgcgcgcg gcaaaatatt tgcgactatg ccgcgcaaca gggttggcag cttcagacac 12300 tgctgcgaga agaacagggc gccttaccca ttactctgtc gggcaatgcc gacgcattct 12360 ggcagcagcg ccccctggcc tgtagtggat tacgtgccgg tctgttccat cctaccaccg 12420 gctattcact gccgctggcg gttgccgtgg ccgaccgcct gagtgcactt gatgtcttta 12480 cgtcggcctc aattcaccat gccattacgc attttgcccg cgagcgctgg cagcagcagg 12540 gctttttccg catgctgaat cgcatgctgt ttttagccgg acccgccgat tcacgctggc 12600 gggttatgca gcgtttttat ggtttacctg aagatttaat tgcccgtttt tatgcgggaa 12660 aactcacgct gaccgatcgg ctacgtattc tgagcggcaa gccgcctgtt ccggtattag 12720 cagcattgca agccattatg acgactcatc gttaactgca ggcatgagta ctgtgtggaa 12780 ttgtgagcgg ataacaattt cacacaggaa acagaccatg gaattcgagc tcatgaagca 12840 actcaccatt ctgggctcga ccggctcgat tggttgcagc acgctggacg tggtgcgcca 12900 taatcccgaa cacttccgcg tagttgcgct ggtggcaggc aaaaatgtca ctcgcatggt 12960 agaacagtgc ctggaattct ctccccgcta tgccgtaatg gacgatgaag cgagtgcgaa 13020 acttcttaaa acgatgctac agcaacaggg tagccgcacc gaagtcttaa gtgggcaaca 13080 agccgcttgc gatatggcag cgcttgagga tgttgatcag gtgatggcag ccattgttgg 13140 cgctgctggg ctgttaccta cgcttgctgc gatccgcgcg ggtaaaacca ttttgctggc 13200 caataaagaa tcactggtta cctgcggacg tctgtttatg gacgccgtaa agcagagcaa 13260 agcgcaattg ttaccggtcg atagcgaaca taacgccatt tttcagagtt taccgcaacc 13320 tatccagcat aatctgggat acgctgacct tgagcaaaat ggcgtggtgt ccattttact 13380 taccgggtct ggtggccctt tccgtgagac gccattgcgc gatttggcaa caatgacgcc 13440 ggatcaagcc tgccgtcatc cgaactggtc gatggggcgt aaaatttctg tcgattcggc 13500 taccatgatg aacaaaggtc tggaatacat tgaagcgcgt tggctgttta acgccagcgc 13560 cagccagatg gaagtgctga ttcacccgca gtcagtgatt cactcaatgg tgcgctatca 13620 ggacggcagt gttctggcgc agctggggga accggatatg cgtacgccaa ttgcccacac 13680 catggcatgg ccgaatcgcg tgaactctgg cgtgaagccg ctcgattttt gcaaactaag 13740 tgcgttgaca tttgccgcac cggattatga tcgttatcca tgcctgaaac tggcgatgga 13800 ggcgttcgaa caaggccagg cagcgacgac agcattgaat gccgcaaacg aaatcaccgt 13860 tgctgctttt cttgcgcaac aaatccgctt tacggatatc gctgcgttga atttatccgt 13920 actggaaaaa atggatatgc gcgaaccaca atgtgtggac gatgtgttat ctgttgatgc 13980 gaacgcgcgt gaagtcgcca gaaaagaggt gatgcgtctc gcaagctgat ctag 14034 <210> 96 <211> 3355 <212> DNA <213> Artificial Sequence <220> <223> pEGFP <400> 96 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 60 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 120 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 180 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg 240 catgcctgca ggtcgactct agaggatccc cgggtaccgg tcgccaccat ggtgagcaag 300 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac 360 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc 420 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc 480 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc 540 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac 600 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc 660 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac 720 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg 780 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag 840 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc 900 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc 960 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccgcgac 1020 tctagaattc caactgagcg ccggtcgcta ccattaccaa cttgtctggt gtcaaaaata 1080 ataggcctac tagtcggccg tacgggccct ttcgtctcgc gcgtttcggt gatgacggtg 1140 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg 1200 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta 1260 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc 1320 acagatgcgt aaggagaaaa taccgcatca ggcggcctta agggcctcgt gatacgccta 1380 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg 1440 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 1500 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 1560 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 1620 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 1680 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 1740 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 1800 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 1860 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 1920 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 1980 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 2040 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 2100 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 2160 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 2220 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 2280 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 2340 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 2400 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 2460 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 2520 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 2580 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 2640 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 2700 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac 2760 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 2820 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 2880 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 2940 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 3000 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 3060 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 3120 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 3180 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 3240 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 3300 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaag 3355 <210> 97 <211> 4616 <212> DNA <213> Artificial Sequence <220> <223> pEGFP-dxr <400> 97 ctagtcggcc gtacgggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct 60 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac 120 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg 180 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg 240 taaggagaaa ataccgcatc aggcggcctt aagggcctcg tgatacgcct atttttatag 300 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg 360 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga 420 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat 480 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca 540 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc 600 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca 660 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg 720 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca 780 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata 840 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag 900 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg 960 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca 1020 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta 1080 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct 1140 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca 1200 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag 1260 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat 1320 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt 1380 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 1440 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 1500 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 1560 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 1620 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 1680 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 1740 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 1800 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 1860 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 1920 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 1980 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 2040 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 2100 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 2160 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 2220 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc 2280 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc 2340 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca 2400 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa 2460 caatttcaca caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc 2520 gactctagag gatccccggg taccggtcgc caccatggtg agcaagggcg aggagctgtt 2580 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag 2640 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg 2700 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt 2760 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat 2820 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac 2880 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat 2940 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca 3000 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg 3060 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat 3120 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag 3180 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg 3240 gatcactctc ggcatggacg agctgtacaa gtaaagcggc cgcgactcta gaattccaac 3300 tgagcgccgg tcgctaccat taccaacttg tctggtgtca aaaataatag gactgtgtgg 3360 aattgtgagc ggataacaat ttcacacagg aaacagacca tggaattcga gctcatgaag 3420 caactcacca ttctgggctc gaccggctcg attggttgca gcacgctgga cgtggtgcgc 3480 cataatcccg aacacttccg cgtagttgcg ctggtggcag gcaaaaatgt cactcgcatg 3540 gtagaacagt gcctggaatt ctctccccgc tatgccgtaa tggacgatga agcgagtgcg 3600 aaacttctta aaacgatgct acagcaacag ggtagccgca ccgaagtctt aagtgggcaa 3660 caagccgctt gcgatatggc agcgcttgag gatgttgatc aggtgatggc agccattgtt 3720 ggcgctgctg ggctgttacc tacgcttgct gcgatccgcg cgggtaaaac cattttgctg 3780 gccaataaag aatcactggt tacctgcgga cgtctgttta tggacgccgt aaagcagagc 3840 aaagcgcaat tgttaccggt cgatagcgaa cataacgcca tttttcagag tttaccgcaa 3900 cctatccagc ataatctggg atacgctgac cttgagcaaa atggcgtggt gtccatttta 3960 cttaccgggt ctggtggccc tttccgtgag acgccattgc gcgatttggc aacaatgacg 4020 ccggatcaag cctgccgtca tccgaactgg tcgatggggc gtaaaatttc tgtcgattcg 4080 gctaccatga tgaacaaagg tctggaatac attgaagcgc gttggctgtt taacgccagc 4140 gccagccaga tggaagtgct gattcacccg cagtcagtga ttcactcaat ggtgcgctat 4200 caggacggca gtgttctggc gcagctgggg gaaccggata tgcgtacgcc aattgcccac 4260 accatggcat ggccgaatcg cgtgaactct ggcgtgaagc cgctcgattt ttgcaaacta 4320 agtgcgttga catttgccgc accggattat gatcgttatc catgcctgaa actggcgatg 4380 gaggcgttcg aacaaggcca ggcagcgacg acagcattga atgccgcaaa cgaaatcacc 4440 gttgctgctt ttcttgcgca acaaatccgc tttacggata tcgctgcgtt gaatttatcc 4500 gtactggaaa aaatggatat gcgcgaacca caatgtgtgg acgatgtgtt atctgttgat 4560 gcgaacgcgc gtgaagtcgc cagaaaagag gtgatgcgtc tcgcaagctg atctag 4616 <210> 98 <211> 2230 <212> DNA <213> Artificial Sequence <220> <223> pORI19 <400> 98 ggaggctcaa gggagtatga gggaatgaaa ttccctcatg ggtttgattt taaaaattgc 60 ttgcaatttt gccgagcggt agcgctggaa aatttttgaa aaaaatttgg aatttggaaa 120 aaaatggggg gaaaggaagc gaattttgct tccgtactac gaccccccat taagtgccga 180 gtgccaattt ttgtgccaaa aacgctctat cccaactggc tcaagggttt aaggggtttt 240 tcaatcgcca acgaatcgcc aacgttttcg ccaacgtttt ttataaatct atatttaagt 300 agctttattg ttgtttttat gattacaaag tgatacacta actttataaa attatttgat 360 tggagttttt taaatggtga tttcagaatc gctagttcta gtgcaccata tgcggtgtga 420 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct 480 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 540 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 600 ttgtaaaacg acggccagtg aattcgagct cggtacccgg ggatcctcta gagtcgacct 660 gcaggcatgc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 720 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 780 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 840 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 900 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 960 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 1020 caggaaagaa catggatctc gacccgtgct ataattatac taattttata aggaggaaaa 1080 aatatgggca tttttagtat ttttgtaatc agcacagttc attatcaacc aaacaaaaaa 1140 taagtggtta taatgaatcg ttaataagca aaattcatat aaccaaatta aagagggtta 1200 taatgaacga gaaaaatata aaacacagtc aaaactttat tacttcaaaa cataatatag 1260 ataaaataat gacaaatata agattaaatg aacatgataa tatctttgaa atcggctcag 1320 gaaaaggcca ttttaccctt gaattagtaa agaggtgtaa tttcgtaact gccattgaaa 1380 tagaccataa attatgcaaa actacagaaa ataaacttgt tgatcacgat aatttccaag 1440 ttttaaacaa ggatatattg cagtttaaat ttcctaaaaa ccaatcctat aaaatatatg 1500 gtaatatacc ttataacata agtacggata taatacgcaa aattgttttt gatagtatag 1560 ctaatgagat ttatttaatc gtggaatacg ggtttgctaa aagattatta aatacaaaac 1620 gctcattggc attactttta atggcagaag ttgatatttc tatattaagt atggttccaa 1680 gagaatattt tcatcctaaa cctaaagtga atagctcact tatcagatta agtagaaaaa 1740 aatcaagaat atcacacaaa gataaacaaa agtataatta tttcgttatg aaatgggtta 1800 acaaagaata caagaaaata tttacaaaaa atcaatttaa caattcctta aaacatgcag 1860 gaattgacga tttaaacaat attagctttg aacaattctt atctcttttc aatagctata 1920 aattatttaa taagtaagtt aagggatgca taaactgcat cccttaactt gtttttcgtg 1980 tgcctatttt ttgtgaatcg ggtcgaggcc tcggactagc gattttttat taaaacgtct 2040 caaaatcgtt tctgagacgt tttagcgttt atttcgttta gttatcggca taatcgttaa 2100 aacaggcgtt atcgtagcgt aaaagccctt gagcgtagcg tggctttgca gcgaagatgt 2160 tgtctgttag attatgaaag ccgatgactg aatgaaataa taagcgcagc gcccttctat 2220 ttcggttgga 2230 <210> 99 <211> 4215 <212> DNA <213> Artificial Sequence <220> <223> pORI19-mvaA <400> 99 ggaggctcaa gggagtatga gggaatgaaa ttccctcatg ggtttgattt taaaaattgc 60 ttgcaatttt gccgagcggt agcgctggaa aatttttgaa aaaaatttgg aatttggaaa 120 aaaatggggg gaaaggaagc gaattttgct tccgtactac gaccccccat taagtgccga 180 gtgccaattt ttgtgccaaa aacgctctat cccaactggc tcaagggttt aaggggtttt 240 tcaatcgcca acgaatcgcc aacgttttcg ccaacgtttt ttataaatct atatttaagt 300 agctttattg ttgtttttat gattacaaag tgatacacta actttataaa attatttgat 360 tggagttttt taaatggtga tttcagaatc gctagttcta gtgcaccata tgcggtgtga 420 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct 480 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 540 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 600 ttgtaaaacg acggccagtg aattcgagct cggtaccagc ttcaaccatt aatgaagttt 660 gtggttcagg gatgaaggca gtaattctag ctaagcaact tttacagttg ggcgaagcag 720 aagttgttat tgctggtgga acggaatcaa tgtctaatgc gcctattctt aaaaatcgta 780 aaactgaaga agaaacatta agcatgttaa gtgacggctt aattgatgct ttttctggca 840 tttcaatggg aattattgga gaaacaattg cggagcagtt tgatgttagt cgtgctgacc 900 aagacctatt cgcacaaaat tctcaagaga aggcagtagc agccagtcaa gcaggaattt 960 ttaatgacga gatcgtagct cttgggaaac ttactgttga tgaaactcct cgaccttcat 1020 ctagcctaga aaaattggct acattacgga cagcctttaa ggaaaatgga acagttacag 1080 ctgggaattc ttcaccagtt aatgacggag cttctgcttt aattttggcc actaaagagt 1140 atgctgaaaa acatggaatt tcttatttag ctgaattagt tgagagtgcg gaagttggta 1200 ttgaccctgc aattatggga gtatcaccga ttgatgcaat tcaaaaatta gttcaacgct 1260 caggcgttca gctttcagaa atagatcttt ttgaaattaa tgaggccttt gctgcatcaa 1320 gtattgctgt taatcgtgaa ttgggcttaa aagattctca agttaatatt tatggaggag 1380 cgattgctct tggacatgct attgggtcaa gtggtgccaa aatcctgaca actttgggat 1440 atgctttgaa acgagaacaa aagcgttatg gaattgctag tctttgtatt ggcggtggtc 1500 tagggcttgc tattttattg aaaaatcctg acttttaaaa attgatggag gaattgccca 1560 gtatttctgg gcatttttgg gaaatatgag aaaaaaattt taactaaaaa atttgagaaa 1620 ataatagcct gtcaagattt tttcttgaca gcatgaaatt agtctgctat aatattaaat 1680 ataataaaaa agaaatagga aatataaaat gtctgtaaaa attcgtttga ctcgtatggg 1740 ttctaagaaa aaacctttct accgtattaa tgttgctgat tcacgtgcac cacgtgatgg 1800 taaattcatc gaaacagttg gaacttacaa tccacttgta actgaaaacc aagtaactct 1860 taaagaagaa cgcgtattgg aatggttgag caacggagca caaccttcag atacagttcg 1920 taacctcctt tcaaaagcgg gcgttatgaa aaaattccac gaatcaaaac tttctaaata 1980 atttttgaga taagctgctt tttagcagct tttcgtgaat ataaaaatca cataaaatgg 2040 acttgaataa ctttcaagtt agagtagagg taagaaatgc aaaaagatgt gaaagaactc 2100 gttttaacta ttgtaaaacc tttggtgact cagcctgatg aagtttcttt ggaaattatt 2160 gaaggtgagg agtttatgga ataccatctc aaagttgctg acggagatat tggaagaata 2220 attggacgtc aaggtcgaat tattcaagcc attcgtacag ttgtatactt tgttcctgtt 2280 gaagggaaaa aagttcgact cctcgtggac caataataag taaaaataaa agaactcatc 2340 attgatgagt tcttttattt ttggaaaacg gaacgttctt ttgagaaata aaatagttat 2400 ttagaatttc cttttattgt ccccaaaaat atgctagaat gaaaaaatat tgattaataa 2460 ttgtagaaat aatcagtgat aaatataaat taactgattt ttatataggt ggataatgga 2520 taagaaaaaa ggaatcttat tagtagcttt agggacgcct cgttcttgtg agacagagga 2580 tgtaagggaa tatttaaaag aatttttggg tgatccactc gtcattcatc ctctagagtc 2640 gacctgcagg catgcaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 2700 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 2760 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 2820 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 2880 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 2940 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 3000 taacgcagga aagaacatgg atctcgaccc gtgctataat tatactaatt ttataaggag 3060 gaaaaaatat gggcattttt agtatttttg taatcagcac agttcattat caaccaaaca 3120 aaaaataagt ggttataatg aatcgttaat aagcaaaatt catataacca aattaaagag 3180 ggttataatg aacgagaaaa atataaaaca cagtcaaaac tttattactt caaaacataa 3240 tatagataaa ataatgacaa atataagatt aaatgaacat gataatatct ttgaaatcgg 3300 ctcaggaaaa ggccatttta cccttgaatt agtaaagagg tgtaatttcg taactgccat 3360 tgaaatagac cataaattat gcaaaactac agaaaataaa cttgttgatc acgataattt 3420 ccaagtttta aacaaggata tattgcagtt taaatttcct aaaaaccaat cctataaaat 3480 atatggtaat ataccttata acataagtac ggatataata cgcaaaattg tttttgatag 3540 tatagctaat gagatttatt taatcgtgga atacgggttt gctaaaagat tattaaatac 3600 aaaacgctca ttggcattac ttttaatggc agaagttgat atttctatat taagtatggt 3660 tccaagagaa tattttcatc ctaaacctaa agtgaatagc tcacttatca gattaagtag 3720 aaaaaaatca agaatatcac acaaagataa acaaaagtat aattatttcg ttatgaaatg 3780 ggttaacaaa gaatacaaga aaatatttac aaaaaatcaa tttaacaatt ccttaaaaca 3840 tgcaggaatt gacgatttaa acaatattag ctttgaacaa ttcttatctc ttttcaatag 3900 ctataaatta tttaataagt aagttaaggg atgcataaac tgcatccctt aacttgtttt 3960 tcgtgtgcct attttttgtg aatcgggtcg aggcctcgga ctagcgattt tttattaaaa 4020 cgtctcaaaa tcgtttctga gacgttttag cgtttatttc gtttagttat cggcataatc 4080 gttaaaacag gcgttatcgt agcgtaaaag cccttgagcg tagcgtggct ttgcagcgaa 4140 gatgttgtct gttagattat gaaagccgat gactgaatga aataataagc gcagcgccct 4200 tctatttcgg ttgga 4215 <210> 100 <211> 5790 <212> DNA <213> Artificial Sequence <220> <223> pCI372 <400> 100 gtaaatcgct cctttttagg tggcacaaat gtgaggcatt ttcgctcttt ccggcgaggc 60 tagttaccct taagttattg gtatgactgg ttttaagcgc aaaaaaagtt gctttttcgt 120 acctattaat gtatcgttag aaaaccgact gtaaaaagta cagtcggcat tatctcatat 180 tataaaagcc agtcattagg cctatctgac aattcctgaa tagagttcat aaacaatcct 240 gcatgataac catcacaaac agaatgatgt acctgtaaag atagcggtaa atatattgaa 300 ttacctttat taatgaattt tcctgctgta ataatgggta gaaggtaatt actattatta 360 ttgatattta agttaaaccc agtaaatgaa gtccatggaa taatagaaag agaaaaagca 420 ttttcaggta taggtgtttt gggaaacaat ttccccgaac cattatattt ctctacatca 480 gaaaggtata aatcataaaa ctctttgaag tcattcttta caggagtcca aataccagag 540 aatgttttag atacaccatc aaaaattgta taaagtggct ctaacttatc ccaataacct 600 aactctccgt cgctattgta accagttcta aaagctgtat ttgagtttat cacccttgtc 660 actaagaaaa taaatgcagg gtaaaattta tatccttctt gttttatgtt tcggtataaa 720 acactaatat caatttctgt ggttatacta aaagtcgttt gttggttcaa ataatgatta 780 aatatctctt ttctcttcca attgtctaaa tcaattttat taaagttcat ttgatatgcc 840 tcctaaattt ttatcttgct cttttgtcag agaaatcata actctttttt tcgattctga 900 aatcaccatt taaaaaactc caatcaaata attttataaa attagtgtat cactttgtaa 960 tcataaaaac aacaataaag ctacttaaat atagatttat aaaaaacgtt ggcgaaaacg 1020 ttggcgattc gttggcgatt gaaaaacccc tcaaaccctt gagccagttg ggatagagcg 1080 tttttggcac aaaaattggc actcggcact taatgggggg tcgtagtacg gaagcaaaat 1140 tcgcttcctt tccccccatt tttttccaaa ttccaaattt ttttcaaaaa ttttccagcg 1200 ctaccgctcg gcaaaattgc aagcaatttt taaaatcaaa cccatgaggg aatttcattc 1260 cctcaaactc ccttgagcct cctccaaccg aaatagaagg acgctgcgct tattatttca 1320 ttcagtcatc ggctttcata atctaacaga caacatcttc gctgcaaagc cacgctacgc 1380 tcaagggctt ttacgctacg ataacgcctg ttttaacgat tatgccgata actaaacgaa 1440 ataaacgcta aaacgtctca gaaacgattt tgagacgttt taataaaaaa tcgacttcgt 1500 tcttttttta cctctcggtt atgagttagt tcaaattcgt tctttttagg ttctaaatcg 1560 tgtttttctt ggaattgtgc tgttttatcc tttaccttgt ctacaaaccc cttaaaaacg 1620 tttttaaagg cttttaagcc gtctgtacgt tccttaagga attcgagctc ggtacccggg 1680 gatcctctag agtcgacctg cagcaatggc aacaacgttg cgcaaactat taactggcga 1740 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc 1800 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 1860 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg 1920 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat 1980 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata 2040 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 2100 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 2160 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 2220 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 2280 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct 2340 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 2400 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 2460 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 2520 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 2580 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 2640 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 2700 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 2760 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 2820 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac 2880 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt 2940 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat 3000 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca 3060 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca 3120 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg 3180 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg 3240 cagatctttt gcccatttta tttttataaa atgggcaggt ggcgtttgtg taaagcaaat 3300 cgacacaatc caaaggggat aaaaggggaa agtgaaactt cccccttttc aagccacatt 3360 gtaatacaag aacgaagtgt tttgtattac aatgtgatag cttgcagtat ttatggtttt 3420 atatggtcta ttttgttgtg aggattgtaa ccgaataggg cgcaatgctt attacaaaat 3480 caatgacaaa gggcgattga ggaatgagcg ctgaggcatt ttatctttga gtaagttatt 3540 gatggatcag aaaaatgtat cacaaattga aacaaagact cactcattta agagaagcta 3600 ctatcatgaa attttgttgt tgtgataagc aacttctaat acacgatttt tagccattac 3660 atcactcgtt tttagagtga tgtgtaagtg cgcattgcac tcttttttta cgaaacaagc 3720 cgaccagcgt ttgaaacttt ttagtttttc atcattctat tttaaaacgt tctaaaactc 3780 gatttaagcg actttaattc gaaactgtct atttgttcaa agggagcatt aagaatgctt 3840 aaacgagctt ttaagggggt ttaaattgat tttgaattga atagcttgtt gtaagttgta 3900 aaaaaaacaa gttaaacaaa gtatcagttt tccatttaag ggttgttagg gcttgccctg 3960 accgtctgta agacgcttga ttgcatgata tgagtattta gctagtcaaa cagttaaaac 4020 agcttatatg agcaattaga gggaatccaa taaattccta aaagcggttt tgatcttttc 4080 ttttagcgag tgaacgctgc aagtaaaatg tgagcgttca ctcgctcact cctttttttg 4140 atgactttga cctttggttt taaatttttg aaaaaaataa aaaataggcg aagcctatta 4200 tatatttatc ttatatattt taatctttta ttcttttgcg tcaaaaaaaa tcaatatttt 4260 caaggcttta tagaattata taccaacaaa aaactgtgta tataccaaca aaaaactgtg 4320 catacaccaa caaaaaactg tgcatatacc aacttctttg tttgtttcgt tggtatataa 4380 tgatataata aaagcatgaa gaatctctct acgaaaagtg tttcttcatg cttatctaaa 4440 ctcactcaca aaggagcagt tttctatgtc tagtatatca aaaaatgaac ctaatcaaaa 4500 gcaggtgcaa accttgaacg aattgtcaaa acgaaaagta gtggaacata attctttaat 4560 taccagtatt gcgaaaatgg ataaaacgcc actgaaaatg tttgaattag ccgtgtcttg 4620 tattaatacc gaagaaccac ccaaagatca tacggtttat ctctcaaaag aagaattgtt 4680 tgcctttttt aaggtatctg ataatgacaa acatagtcgt tttaaacaag cagtagagaa 4740 tatgcaaaaa caagcctttt ttcaaattaa agaagaagta ggtaaaggat ttaaatttag 4800 gagtattgtt cccattccat atgtcgagtg gacagattat catgatgacg taaaaattga 4860 atttcatcgt gaaatcatgc cctacttaat taatctaaaa caaaatttca cgcaacatgc 4920 tttgtctgat attgcagagc tgaatagcaa atactctatt atcttgtacc gttggttatc 4980 catgaattat aaccaatacg agcattatag ttataagggc ggacggagag aagaacaagt 5040 ggaagcctac cgcaatccta ccatttcaat gcgagaatta cgagaaatga cggatacagt 5100 tgatgaatac ccccgctttg atagattaga acatagagtt ttaaaagaac caatagaaga 5160 aattaacgaa aacacctctt ttaacgtgac gtatgacaag ataaaaaaag gacgaagcat 5220 tgattctatt gtctttcata tcacgaaaaa acgtcgagca gatgataaca gctacaagtt 5280 agaagataaa gattatcaat ccgacaaaga ggaaaaatca agaaatgaag ctgacttatt 5340 aaaacaggca atggaaagca agtacacacg attattgatt gaaaactttc tcttatcccc 5400 tcttgaaatg acggacacgg cacttatggc aggtttgcaa aagaacgtct atccgttgta 5460 tgacgagtta aaggaattaa gaggattgaa tggggtcaaa gaccacttgt cttatatatc 5520 tagcaaaaaa gaagcctatt ctaaacgcaa tgtagcgaag tatctgaaaa aagcaatcga 5580 gcaatatcta cctacggtta aaaggcagga cttaaaccat gagtgagaac ttaaaaacga 5640 ttaaagagtt ggctgatgag ttgggcgtat caaaaaagaa aattcattat caagtatcta 5700 aattagatag tgatttgata caaaaattag acggcactat atatctagct gatttcactt 5760 tttgcattct acaaactgca taactcatat 5790 <210> 101 <211> 6350 <212> DNA <213> Artificial Sequence <220> <223> pCIN <400> 101 gtaaatcgct cctttttagg tggcacaaat gtgaggcatt ttcgctcttt ccggcgaggc 60 tagttaccct taagttattg gtatgactgg ttttaagcgc aaaaaaagtt gctttttcgt 120 acctattaat gtatcgttag aaaaccgact gtaaaaagta cagtcggcat tatctcatat 180 tataaaagcc agtcattagg cctatctgac aattcctgaa tagagttcat aaacaatcct 240 gcatgataac catcacaaac agaatgatgt acctgtaaag atagcggtaa atatattgaa 300 ttacctttat taatgaattt tcctgctgta ataatgggta gaaggtaatt actattatta 360 ttgatattta agttaaaccc agtaaatgaa gtccatggaa taatagaaag agaaaaagca 420 ttttcaggta taggtgtttt gggaaacaat ttccccgaac cattatattt ctctacatca 480 gaaaggtata aatcataaaa ctctttgaag tcattcttta caggagtcca aataccagag 540 aatgttttag atacaccatc aaaaattgta taaagtggct ctaacttatc ccaataacct 600 aactctccgt cgctattgta accagttcta aaagctgtat ttgagtttat cacccttgtc 660 actaagaaaa taaatgcagg gtaaaattta tatccttctt gttttatgtt tcggtataaa 720 acactaatat caatttctgt ggttatacta aaagtcgttt gttggttcaa ataatgatta 780 aatatctctt ttctcttcca attgtctaaa tcaattttat taaagttcat ttgatatgcc 840 tcctaaattt ttatcttgct cttttgtcag agaaatcata actctttttt tcgattctga 900 aatcaccatt taaaaaactc caatcaaata attttataaa attagtgtat cactttgtaa 960 tcataaaaac aacaataaag ctacttaaat atagatttat aaaaaacgtt ggcgaaaacg 1020 ttggcgattc gttggcgatt gaaaaacccc tcaaaccctt gagccagttg ggatagagcg 1080 tttttggcac aaaaattggc actcggcact taatgggggg tcgtagtacg gaagcaaaat 1140 tcgcttcctt tccccccatt tttttccaaa ttccaaattt ttttcaaaaa ttttccagcg 1200 ctaccgctcg gcaaaattgc aagcaatttt taaaatcaaa cccatgaggg aatttcattc 1260 cctcaaactc ccttgagcct cctccaaccg aaatagaagg acgctgcgct tattatttca 1320 ttcagtcatc ggctttcata atctaacaga caacatcttc gctgcaaagc cacgctacgc 1380 tcaagggctt ttacgctacg ataacgcctg ttttaacgat tatgccgata actaaacgaa 1440 ataaacgcta aaacgtctca gaaacgattt tgagacgttt taataaaaaa tcgacttcgt 1500 tcttttttta cctctcggtt atgagttagt tcaaattcgt tctttttagg ttctaaatcg 1560 tgtttttctt ggaattgtgc tgttttatcc tttaccttgt ctacaaaccc cttaaaaacg 1620 tttttaaagg cttttaagcc gtctgtacgt tccttaaggg ctagcataga ttagtttatt 1680 cttgacacta caagctaaat gtggtataat cccatagagt gtggaattgt gagcggataa 1740 caatttcaca caggaaacag accatggaat tcgagctcgg tacccgggga tcctctagag 1800 tcgacctgca ggcatgcaag cttggctgtt ttggcggatg agagaagatt ttcagcctga 1860 tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct ggcggcagta 1920 gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg 1980 gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag 2040 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg 2100 agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg 2160 cgggcaggac gcccgccata aactgccagg catcaaatta agcagaaggc catcctgacg 2220 gatggccttt ttgcgtttct acaaactctt accggtcctg cagcaatggc aacaacgttg 2280 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg 2340 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt 2400 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg 2460 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg 2520 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg 2580 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa 2640 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 2700 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 2760 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 2820 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 2880 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta 2940 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 3000 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 3060 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 3120 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 3180 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 3240 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 3300 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 3360 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 3420 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg 3480 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc 3540 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct 3600 gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg 3660 cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat 3720 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt 3780 catcaccgaa acgcgcgagg cagatctttt gcccatttta tttttataaa atgggcaggt 3840 ggcgtttgtg taaagcaaat cgacacaatc caaaggggat aaaaggggaa agtgaaactt 3900 cccccttttc aagccacatt gtaatacaag aacgaagtgt tttgtattac aatgtgatag 3960 cttgcagtat ttatggtttt atatggtcta ttttgttgtg aggattgtaa ccgaataggg 4020 cgcaatgctt attacaaaat caatgacaaa gggcgattga ggaatgagcg ctgaggcatt 4080 ttatctttga gtaagttatt gatggatcag aaaaatgtat cacaaattga aacaaagact 4140 cactcattta agagaagcta ctatcatgaa attttgttgt tgtgataagc aacttctaat 4200 acacgatttt tagccattac atcactcgtt tttagagtga tgtgtaagtg cgcattgcac 4260 tcttttttta cgaaacaagc cgaccagcgt ttgaaacttt ttagtttttc atcattctat 4320 tttaaaacgt tctaaaactc gatttaagcg actttaattc gaaactgtct atttgttcaa 4380 agggagcatt aagaatgctt aaacgagctt ttaagggggt ttaaattgat tttgaattga 4440 atagcttgtt gtaagttgta aaaaaaacaa gttaaacaaa gtatcagttt tccatttaag 4500 ggttgttagg gcttgccctg accgtctgta agacgcttga ttgcatgata tgagtattta 4560 gctagtcaaa cagttaaaac agcttatatg agcaattaga gggaatccaa taaattccta 4620 aaagcggttt tgatcttttc ttttagcgag tgaacgctgc aagtaaaatg tgagcgttca 4680 ctcgctcact cctttttttg atgactttga cctttggttt taaatttttg aaaaaaataa 4740 aaaataggcg aagcctatta tatatttatc ttatatattt taatctttta ttcttttgcg 4800 tcaaaaaaaa tcaatatttt caaggcttta tagaattata taccaacaaa aaactgtgta 4860 tataccaaca aaaaactgtg catacaccaa caaaaaactg tgcatatacc aacttctttg 4920 tttgtttcgt tggtatataa tgatataata aaagcatgaa gaatctctct acgaaaagtg 4980 tttcttcatg cttatctaaa ctcactcaca aaggagcagt tttctatgtc tagtatatca 5040 aaaaatgaac ctaatcaaaa gcaggtgcaa accttgaacg aattgtcaaa acgaaaagta 5100 gtggaacata attctttaat taccagtatt gcgaaaatgg ataaaacgcc actgaaaatg 5160 tttgaattag ccgtgtcttg tattaatacc gaagaaccac ccaaagatca tacggtttat 5220 ctctcaaaag aagaattgtt tgcctttttt aaggtatctg ataatgacaa acatagtcgt 5280 tttaaacaag cagtagagaa tatgcaaaaa caagcctttt ttcaaattaa agaagaagta 5340 ggtaaaggat ttaaatttag gagtattgtt cccattccat atgtcgagtg gacagattat 5400 catgatgacg taaaaattga atttcatcgt gaaatcatgc cctacttaat taatctaaaa 5460 caaaatttca cgcaacatgc tttgtctgat attgcagagc tgaatagcaa atactctatt 5520 atcttgtacc gttggttatc catgaattat aaccaatacg agcattatag ttataagggc 5580 ggacggagag aagaacaagt ggaagcctac cgcaatccta ccatttcaat gcgagaatta 5640 cgagaaatga cggatacagt tgatgaatac ccccgctttg atagattaga acatagagtt 5700 ttaaaagaac caatagaaga aattaacgaa aacacctctt ttaacgtgac gtatgacaag 5760 ataaaaaaag gacgaagcat tgattctatt gtctttcata tcacgaaaaa acgtcgagca 5820 gatgataaca gctacaagtt agaagataaa gattatcaat ccgacaaaga ggaaaaatca 5880 agaaatgaag ctgacttatt aaaacaggca atggaaagca agtacacacg attattgatt 5940 gaaaactttc tcttatcccc tcttgaaatg acggacacgg cacttatggc aggtttgcaa 6000 aagaacgtct atccgttgta tgacgagtta aaggaattaa gaggattgaa tggggtcaaa 6060 gaccacttgt cttatatatc tagcaaaaaa gaagcctatt ctaaacgcaa tgtagcgaag 6120 tatctgaaaa aagcaatcga gcaatatcta cctacggtta aaaggcagga cttaaaccat 6180 gagtgagaac ttaaaaacga ttaaagagtt ggctgatgag ttgggcgtat caaaaaagaa 6240 aattcattat caagtatcta aattagatag tgatttgata caaaaattag acggcactat 6300 atatctagct gatttcactt tttgcattct acaaactgca taactcatat 6350 <210> 102 <211> 7597 <212> DNA <213> Artificial Sequence <220> <223> pCIN-mvaA <400> 102 gtaaatcgct cctttttagg tggcacaaat gtgaggcatt ttcgctcttt ccggcgaggc 60 tagttaccct taagttattg gtatgactgg ttttaagcgc aaaaaaagtt gctttttcgt 120 acctattaat gtatcgttag aaaaccgact gtaaaaagta cagtcggcat tatctcatat 180 tataaaagcc agtcattagg cctatctgac aattcctgaa tagagttcat aaacaatcct 240 gcatgataac catcacaaac agaatgatgt acctgtaaag atagcggtaa atatattgaa 300 ttacctttat taatgaattt tcctgctgta ataatgggta gaaggtaatt actattatta 360 ttgatattta agttaaaccc agtaaatgaa gtccatggaa taatagaaag agaaaaagca 420 ttttcaggta taggtgtttt gggaaacaat ttccccgaac cattatattt ctctacatca 480 gaaaggtata aatcataaaa ctctttgaag tcattcttta caggagtcca aataccagag 540 aatgttttag atacaccatc aaaaattgta taaagtggct ctaacttatc ccaataacct 600 aactctccgt cgctattgta accagttcta aaagctgtat ttgagtttat cacccttgtc 660 actaagaaaa taaatgcagg gtaaaattta tatccttctt gttttatgtt tcggtataaa 720 acactaatat caatttctgt ggttatacta aaagtcgttt gttggttcaa ataatgatta 780 aatatctctt ttctcttcca attgtctaaa tcaattttat taaagttcat ttgatatgcc 840 tcctaaattt ttatcttgct cttttgtcag agaaatcata actctttttt tcgattctga 900 aatcaccatt taaaaaactc caatcaaata attttataaa attagtgtat cactttgtaa 960 tcataaaaac aacaataaag ctacttaaat atagatttat aaaaaacgtt ggcgaaaacg 1020 ttggcgattc gttggcgatt gaaaaacccc tcaaaccctt gagccagttg ggatagagcg 1080 tttttggcac aaaaattggc actcggcact taatgggggg tcgtagtacg gaagcaaaat 1140 tcgcttcctt tccccccatt tttttccaaa ttccaaattt ttttcaaaaa ttttccagcg 1200 ctaccgctcg gcaaaattgc aagcaatttt taaaatcaaa cccatgaggg aatttcattc 1260 cctcaaactc ccttgagcct cctccaaccg aaatagaagg acgctgcgct tattatttca 1320 ttcagtcatc ggctttcata atctaacaga caacatcttc gctgcaaagc cacgctacgc 1380 tcaagggctt ttacgctacg ataacgcctg ttttaacgat tatgccgata actaaacgaa 1440 ataaacgcta aaacgtctca gaaacgattt tgagacgttt taataaaaaa tcgacttcgt 1500 tcttttttta cctctcggtt atgagttagt tcaaattcgt tctttttagg ttctaaatcg 1560 tgtttttctt ggaattgtgc tgttttatcc tttaccttgt ctacaaaccc cttaaaaacg 1620 tttttaaagg cttttaagcc gtctgtacgt tccttaaggg ctagcataga ttagtttatt 1680 cttgacacta caagctaaat gtggtataat cccatagagt gtggaattgt gagcggataa 1740 caatttcaca caggaaacag accatggaat tcgagctcgg tacccgggga tccaggaggt 1800 aataaatatg agaaaaaaat tttatcaaat gtcgccacaa gagcgattga attcgttaaa 1860 cttatccgaa aagagtcaag aaattttaag tgagatggcc ctagatacaa ctattttgga 1920 caatctcata gaaaatcaaa tttctgaatt tgaattgcca atgggaattg cccaaaattt 1980 tgttattaat ggacaaagtt ttctaatacc aatggttaca gaggaaccgt cagtaattgc 2040 cgcagcaagt aatggagcaa aaatagctgg aaattttgta gctgaaatta aagaacgttt 2100 aatgcgtggt caaattgtat tttatgatgt taaaaattca gataagattg caaatgaaat 2160 acttgaaaag caagaaaaaa tctttgaaca agccgaactt tcttaccctt caatcgttaa 2220 acgaggtggc ggtttgagag aggtttctag tcggattttc tctagtcaaa agtttttatc 2280 tgttgatgtc aaggttgatg ttaaagacgc catgggtgca aatattatta attcaatctt 2340 agagggaatt gctgaactct ttcggaggtg gtttcctgat gaaaaaattc tgtttagtat 2400 tttatcaaat tatgctactg aatcactagt gaaagtgacc tgtgaaattc cggttgaacg 2460 cctttcaaaa aaagcagatg gatatgaaat tggtcaaaaa attatggctg cttcccaata 2520 ttcaaaaatt gatccttatc gtgctagcac ccataacaaa gggattatga acggtatcaa 2580 tgcggtcatt ttagctacag gaaatgatac tcgggccatc tcagctgcta ttcatgctta 2640 tgcggcaaaa gatggagcct accaagggtt agccaactgg gaacttcaag agaaaatgtt 2700 agttggcgaa ctcgaatttc ctttaccagt ggcaactgtc ggagggggag tcaaggtttt 2760 gccaaaagca caagcggcca tggagattct gggtatcagt gatgctaagg agctcgcaaa 2820 agttattgct gcagtgggac tagctcaaaa cttagccgct ttacgtgccc tagtttcaga 2880 aggaattcaa cagggacaca tgagtctaca agctcgttct ttggctttga gtgtaggagc 2940 acaagcggat gaaatcgcga tattaagtca acaattgcgc aaagaaaaag tgatgaatca 3000 agaagtagcc caaaatttac taaaaaattt gagaaaataa tctagagtcg acctgcaggc 3060 atgcaagctt ggctgttttg gcggatgaga gaagattttc agcctgatac agattaaatc 3120 agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg cggtggtccc 3180 acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggtc 3240 tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcgaaag 3300 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc 3360 cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc 3420 cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat ggcctttttg 3480 cgtttctaca aactcttacc ggtcctgcag caatggcaac aacgttgcgc aaactattaa 3540 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata 3600 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat 3660 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc 3720 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata 3780 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt 3840 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga 3900 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 3960 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 4020 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 4080 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 4140 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 4200 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 4260 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 4320 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 4380 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 4440 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 4500 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 4560 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 4620 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc 4680 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg 4740 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt 4800 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt 4860 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc 4920 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca 4980 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg 5040 cgcgaggcag atcttttgcc cattttattt ttataaaatg ggcaggtggc gtttgtgtaa 5100 agcaaatcga cacaatccaa aggggataaa aggggaaagt gaaacttccc ccttttcaag 5160 ccacattgta atacaagaac gaagtgtttt gtattacaat gtgatagctt gcagtattta 5220 tggttttata tggtctattt tgttgtgagg attgtaaccg aatagggcgc aatgcttatt 5280 acaaaatcaa tgacaaaggg cgattgagga atgagcgctg aggcatttta tctttgagta 5340 agttattgat ggatcagaaa aatgtatcac aaattgaaac aaagactcac tcatttaaga 5400 gaagctacta tcatgaaatt ttgttgttgt gataagcaac ttctaataca cgatttttag 5460 ccattacatc actcgttttt agagtgatgt gtaagtgcgc attgcactct ttttttacga 5520 aacaagccga ccagcgtttg aaacttttta gtttttcatc attctatttt aaaacgttct 5580 aaaactcgat ttaagcgact ttaattcgaa actgtctatt tgttcaaagg gagcattaag 5640 aatgcttaaa cgagctttta agggggttta aattgatttt gaattgaata gcttgttgta 5700 agttgtaaaa aaaacaagtt aaacaaagta tcagttttcc atttaagggt tgttagggct 5760 tgccctgacc gtctgtaaga cgcttgattg catgatatga gtatttagct agtcaaacag 5820 ttaaaacagc ttatatgagc aattagaggg aatccaataa attcctaaaa gcggttttga 5880 tcttttcttt tagcgagtga acgctgcaag taaaatgtga gcgttcactc gctcactcct 5940 ttttttgatg actttgacct ttggttttaa atttttgaaa aaaataaaaa ataggcgaag 6000 cctattatat atttatctta tatattttaa tcttttattc ttttgcgtca aaaaaaatca 6060 atattttcaa ggctttatag aattatatac caacaaaaaa ctgtgtatat accaacaaaa 6120 aactgtgcat acaccaacaa aaaactgtgc atataccaac ttctttgttt gtttcgttgg 6180 tatataatga tataataaaa gcatgaagaa tctctctacg aaaagtgttt cttcatgctt 6240 atctaaactc actcacaaag gagcagtttt ctatgtctag tatatcaaaa aatgaaccta 6300 atcaaaagca ggtgcaaacc ttgaacgaat tgtcaaaacg aaaagtagtg gaacataatt 6360 ctttaattac cagtattgcg aaaatggata aaacgccact gaaaatgttt gaattagccg 6420 tgtcttgtat taataccgaa gaaccaccca aagatcatac ggtttatctc tcaaaagaag 6480 aattgtttgc cttttttaag gtatctgata atgacaaaca tagtcgtttt aaacaagcag 6540 tagagaatat gcaaaaacaa gccttttttc aaattaaaga agaagtaggt aaaggattta 6600 aatttaggag tattgttccc attccatatg tcgagtggac agattatcat gatgacgtaa 6660 aaattgaatt tcatcgtgaa atcatgccct acttaattaa tctaaaacaa aatttcacgc 6720 aacatgcttt gtctgatatt gcagagctga atagcaaata ctctattatc ttgtaccgtt 6780 ggttatccat gaattataac caatacgagc attatagtta taagggcgga cggagagaag 6840 aacaagtgga agcctaccgc aatcctacca tttcaatgcg agaattacga gaaatgacgg 6900 atacagttga tgaatacccc cgctttgata gattagaaca tagagtttta aaagaaccaa 6960 tagaagaaat taacgaaaac acctctttta acgtgacgta tgacaagata aaaaaaggac 7020 gaagcattga ttctattgtc tttcatatca cgaaaaaacg tcgagcagat gataacagct 7080 acaagttaga agataaagat tatcaatccg acaaagagga aaaatcaaga aatgaagctg 7140 acttattaaa acaggcaatg gaaagcaagt acacacgatt attgattgaa aactttctct 7200 tatcccctct tgaaatgacg gacacggcac ttatggcagg tttgcaaaag aacgtctatc 7260 cgttgtatga cgagttaaag gaattaagag gattgaatgg ggtcaaagac cacttgtctt 7320 atatatctag caaaaaagaa gcctattcta aacgcaatgt agcgaagtat ctgaaaaaag 7380 caatcgagca atatctacct acggttaaaa ggcaggactt aaaccatgag tgagaactta 7440 aaaacgatta aagagttggc tgatgagttg ggcgtatcaa aaaagaaaat tcattatcaa 7500 gtatctaaat tagatagtga tttgatacaa aaattagacg gcactatata tctagctgat 7560 ttcacttttt gcattctaca aactgcataa ctcatat 7597 <210> 103 <211> 8327 <212> DNA <213> Artificial Sequence <220> <223> pCIN-mvaA-EGFP <400> 103 gtaaatcgct cctttttagg tggcacaaat gtgaggcatt ttcgctcttt ccggcgaggc 60 tagttaccct taagttattg gtatgactgg ttttaagcgc aaaaaaagtt gctttttcgt 120 acctattaat gtatcgttag aaaaccgact gtaaaaagta cagtcggcat tatctcatat 180 tataaaagcc agtcattagg cctatctgac aattcctgaa tagagttcat aaacaatcct 240 gcatgataac catcacaaac agaatgatgt acctgtaaag atagcggtaa atatattgaa 300 ttacctttat taatgaattt tcctgctgta ataatgggta gaaggtaatt actattatta 360 ttgatattta agttaaaccc agtaaatgaa gtccatggaa taatagaaag agaaaaagca 420 ttttcaggta taggtgtttt gggaaacaat ttccccgaac cattatattt ctctacatca 480 gaaaggtata aatcataaaa ctctttgaag tcattcttta caggagtcca aataccagag 540 aatgttttag atacaccatc aaaaattgta taaagtggct ctaacttatc ccaataacct 600 aactctccgt cgctattgta accagttcta aaagctgtat ttgagtttat cacccttgtc 660 actaagaaaa taaatgcagg gtaaaattta tatccttctt gttttatgtt tcggtataaa 720 acactaatat caatttctgt ggttatacta aaagtcgttt gttggttcaa ataatgatta 780 aatatctctt ttctcttcca attgtctaaa tcaattttat taaagttcat ttgatatgcc 840 tcctaaattt ttatcttgct cttttgtcag agaaatcata actctttttt tcgattctga 900 aatcaccatt taaaaaactc caatcaaata attttataaa attagtgtat cactttgtaa 960 tcataaaaac aacaataaag ctacttaaat atagatttat aaaaaacgtt ggcgaaaacg 1020 ttggcgattc gttggcgatt gaaaaacccc tcaaaccctt gagccagttg ggatagagcg 1080 tttttggcac aaaaattggc actcggcact taatgggggg tcgtagtacg gaagcaaaat 1140 tcgcttcctt tccccccatt tttttccaaa ttccaaattt ttttcaaaaa ttttccagcg 1200 ctaccgctcg gcaaaattgc aagcaatttt taaaatcaaa cccatgaggg aatttcattc 1260 cctcaaactc ccttgagcct cctccaaccg aaatagaagg acgctgcgct tattatttca 1320 ttcagtcatc ggctttcata atctaacaga caacatcttc gctgcaaagc cacgctacgc 1380 tcaagggctt ttacgctacg ataacgcctg ttttaacgat tatgccgata actaaacgaa 1440 ataaacgcta aaacgtctca gaaacgattt tgagacgttt taataaaaaa tcgacttcgt 1500 tcttttttta cctctcggtt atgagttagt tcaaattcgt tctttttagg ttctaaatcg 1560 tgtttttctt ggaattgtgc tgttttatcc tttaccttgt ctacaaaccc cttaaaaacg 1620 tttttaaagg cttttaagcc gtctgtacgt tccttaaggg ctagcataga ttagtttatt 1680 cttgacacta caagctaaat gtggtataat cccatagagt gtggaattgt gagcggataa 1740 caatttcaca caggaaacag accatggaat tcgagctcgg tacccgggga tccaggaggt 1800 aataaatatg agaaaaaaat tttatcaaat gtcgccacaa gagcgattga attcgttaaa 1860 cttatccgaa aagagtcaag aaattttaag tgagatggcc ctagatacaa ctattttgga 1920 caatctcata gaaaatcaaa tttctgaatt tgaattgcca atgggaattg cccaaaattt 1980 tgttattaat ggacaaagtt ttctaatacc aatggttaca gaggaaccgt cagtaattgc 2040 cgcagcaagt aatggagcaa aaatagctgg aaattttgta gctgaaatta aagaacgttt 2100 aatgcgtggt caaattgtat tttatgatgt taaaaattca gataagattg caaatgaaat 2160 acttgaaaag caagaaaaaa tctttgaaca agccgaactt tcttaccctt caatcgttaa 2220 acgaggtggc ggtttgagag aggtttctag tcggattttc tctagtcaaa agtttttatc 2280 tgttgatgtc aaggttgatg ttaaagacgc catgggtgca aatattatta attcaatctt 2340 agagggaatt gctgaactct ttcggaggtg gtttcctgat gaaaaaattc tgtttagtat 2400 tttatcaaat tatgctactg aatcactagt gaaagtgacc tgtgaaattc cggttgaacg 2460 cctttcaaaa aaagcagatg gatatgaaat tggtcaaaaa attatggctg cttcccaata 2520 ttcaaaaatt gatccttatc gtgctagcac ccataacaaa gggattatga acggtatcaa 2580 tgcggtcatt ttagctacag gaaatgatac tcgggccatc tcagctgcta ttcatgctta 2640 tgcggcaaaa gatggagcct accaagggtt agccaactgg gaacttcaag agaaaatgtt 2700 agttggcgaa ctcgaatttc ctttaccagt ggcaactgtc ggagggggag tcaaggtttt 2760 gccaaaagca caagcggcca tggagattct gggtatcagt gatgctaagg agctcgcaaa 2820 agttattgct gcagtgggac tagctcaaaa cttagccgct ttacgtgccc tagtttcaga 2880 aggaattcaa cagggacaca tgagtctaca agctcgttct ttggctttga gtgtaggagc 2940 acaagcggat gaaatcgcga tattaagtca acaattgcgc aaagaaaaag tgatgaatca 3000 agaagtagcc caaaatttac taaaaaattt gagaaaataa tctagagtcg acaggaggta 3060 ataaatatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag 3120 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc 3180 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg 3240 cccaccctcg tgaccaccct gacctggggc gtgcagtgct tcagccgcta ccccgaccac 3300 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc 3360 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac 3420 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg 3480 gggcacaagc tggagtacaa ctacatcagc cacaacgtct atatcaccgc cgacaagcag 3540 aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag 3600 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac 3660 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac 3720 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac 3780 aagtaagcat gcaagcttgg ctgttttggc ggatgagaga agattttcgg cctgatacag 3840 attaaatcag aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg 3900 gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt 3960 gtggggtctc ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc 4020 agtcgaaaga ctggggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt 4080 aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg 4140 gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat 4200 ggcctttttg cgtttctaca aactcttacc ggtcctgcag caatggcaac aacgttgcgc 4260 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg 4320 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt 4380 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca 4440 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat 4500 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca 4560 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 4620 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 4680 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 4740 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 4800 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 4860 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 4920 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 4980 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 5040 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 5100 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 5160 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 5220 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 5280 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 5340 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 5400 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc 5460 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt 5520 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat 5580 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc 5640 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg 5700 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat 5760 caccgaaacg cgcgaggcag atcttttgcc cattttattt ttataaaatg ggcaggtggc 5820 gtttgtgtaa agcaaatcga cacaatccaa aggggataaa aggggaaagt gaaacttccc 5880 ccttttcaag ccacattgta atacaagaac gaagtgtttt gtattacaat gtgatagctt 5940 gcagtattta tggttttata tggtctattt tgttgtgagg attgtaaccg aatagggcgc 6000 aatgcttatt acaaaatcaa tgacaaaggg cgattgagga atgagcgctg aggcatttta 6060 tctttgagta agttattgat ggatcagaaa aatgtatcac aaattgaaac aaagactcac 6120 tcatttaaga gaagctacta tcatgaaatt ttgttgttgt gataagcaac ttctaataca 6180 cgatttttag ccattacatc actcgttttt agagtgatgt gtaagtgcgc attgcactct 6240 ttttttacga aacaagccga ccagcgtttg aaacttttta gtttttcatc attctatttt 6300 aaaacgttct aaaactcgat ttaagcgact ttaattcgaa actgtctatt tgttcaaagg 6360 gagcattaag aatgcttaaa cgagctttta agggggttta aattgatttt gaattgaata 6420 gcttgttgta agttgtaaaa aaaacaagtt aaacaaagta tcagttttcc atttaagggt 6480 tgttagggct tgccctgacc gtctgtaaga cgcttgattg catgatatga gtatttagct 6540 agtcaaacag ttaaaacagc ttatatgagc aattagaggg aatccaataa attcctaaaa 6600 gcggttttga tcttttcttt tagcgagtga acgctgcaag taaaatgtga gcgttcactc 6660 gctcactcct ttttttgatg actttgacct ttggttttaa atttttgaaa aaaataaaaa 6720 ataggcgaag cctattatat atttatctta tatattttaa tcttttattc ttttgcgtca 6780 aaaaaaatca atattttcaa ggctttatag aattatatac caacaaaaaa ctgtgtatat 6840 accaacaaaa aactgtgcat acaccaacaa aaaactgtgc atataccaac ttctttgttt 6900 gtttcgttgg tatataatga tataataaaa gcatgaagaa tctctctacg aaaagtgttt 6960 cttcatgctt atctaaactc actcacaaag gagcagtttt ctatgtctag tatatcaaaa 7020 aatgaaccta atcaaaagca ggtgcaaacc ttgaacgaat tgtcaaaacg aaaagtagtg 7080 gaacataatt ctttaattac cagtattgcg aaaatggata aaacgccact gaaaatgttt 7140 gaattagccg tgtcttgtat taataccgaa gaaccaccca aagatcatac ggtttatctc 7200 tcaaaagaag aattgtttgc cttttttaag gtatctgata atgacaaaca tagtcgtttt 7260 aaacaagcag tagagaatat gcaaaaacaa gccttttttc aaattaaaga agaagtaggt 7320 aaaggattta aatttaggag tattgttccc attccatatg tcgagtggac agattatcat 7380 gatgacgtaa aaattgaatt tcatcgtgaa atcatgccct acttaattaa tctaaaacaa 7440 aatttcacgc aacatgcttt gtctgatatt gcagagctga atagcaaata ctctattatc 7500 ttgtaccgtt ggttatccat gaattataac caatacgagc attatagtta taagggcgga 7560 cggagagaag aacaagtgga agcctaccgc aatcctacca tttcaatgcg agaattacga 7620 gaaatgacgg atacagttga tgaatacccc cgctttgata gattagaaca tagagtttta 7680 aaagaaccaa tagaagaaat taacgaaaac acctctttta acgtgacgta tgacaagata 7740 aaaaaaggac gaagcattga ttctattgtc tttcatatca cgaaaaaacg tcgagcagat 7800 gataacagct acaagttaga agataaagat tatcaatccg acaaagagga aaaatcaaga 7860 aatgaagctg acttattaaa acaggcaatg gaaagcaagt acacacgatt attgattgaa 7920 aactttctct tatcccctct tgaaatgacg gacacggcac ttatggcagg tttgcaaaag 7980 aacgtctatc cgttgtatga cgagttaaag gaattaagag gattgaatgg ggtcaaagac 8040 cacttgtctt atatatctag caaaaaagaa gcctattcta aacgcaatgt agcgaagtat 8100 ctgaaaaaag caatcgagca atatctacct acggttaaaa ggcaggactt aaaccatgag 8160 tgagaactta aaaacgatta aagagttggc tgatgagttg ggcgtatcaa aaaagaaaat 8220 tcattatcaa gtatctaaat tagatagtga tttgatacaa aaattagacg gcactatata 8280 tctagctgat ttcacttttt gcattctaca aactgcataa ctcatat 8327 <210> 104 <211> 2412 <212> DNA <213> Enterococcus faecalis mvaE <400> 104 ttgaaaacag tagttattat tgatgcatta cgaacaccaa ttggaaaata taaaggcagc 60 ttaagtcaag taagtgccgt agacttagga acacatgtta caacacaact tttaaaaaga 120 cattccacta tttctgaaga aattgatcaa gtaatctttg gaaatgtttt acaagctgga 180 aatggccaaa atcccgcacg acaaatagca ataaacagcg gtttatctca tgaaattccc 240 gcaatgacag ttaatgaggt ctgcggatca ggaatgaagg ccgttatttt ggcgaaacaa 300 ttgattcaat taggagaagc ggaagtttta attgctggcg ggattgagaa tatgtcccaa 360 gcacctaaat tacaacgatt taattacgaa acagaaagct atgatgcgcc tttttctagt 420 atgatgtacg atgggttaac ggatgccttt agtggtcaag caatgggctt aactgctgaa 480 aatgtggccg aaaagtatca tgtaactaga gaagagcaag atcaattttc tgtacattca 540 caattaaaag cagctcaagc acaagcagaa gggatattcg ctgacgaaat agccccatta 600 gaagtatcag gaacgcttgt ggagaaagat gaagggattc gccctaattc gagcgttgag 660 aagctaggaa cgcttaaaac agtttttaaa gaagacggta ctgtaacagc agggaatgca 720 tcaaccatta atgatggggc ttctgctttg attattgctt cacaagaata tgccgaagca 780 cacggtcttc cttatttagc tattattcga gacagtgtgg aagtcggtat tgatccagcc 840 tatatgggaa tttcgccgat taaagccatt caaaaactgt tagcgcgcaa tcaacttact 900 acggaagaaa ttgatctgta tgaaatcaac gaagcatttg cagcaacttc aatcgtggtc 960 caaagagaac tggctttacc agaggaaaag gtcaacattt atggtggcgg tatttcatta 1020 ggtcatgcga ttggtgccac aggtgctcgt ttattaacga gtttaagtta tcaattaaat 1080 caaaaagaaa agaaatatgg agtggcttct ttatgtatcg gcggtggctt aggactcgct 1140 atgctactag agagacctca gcaaaaaaaa aacagccgat tttatcaaat gagtcctgag 1200 gaacgcctgg cttctcttct taatgaaggc cagatttctg ctgatacaaa aaaagaattt 1260 gaaaatacgg ctttatcttc gcagattgcc aatcatatga ttgaaaatca aatcagtgaa 1320 acagaagtgc cgatgggcgt tggcttacat ttaacagtgg acgaaactga ttatttggta 1380 ccaatggcga cagaagagcc ctcagtgatt gcggctttga gtaatggtgc aaaaatagca 1440 caaggattta aaacagtgaa tcaacaacgt ttaatgcgtg gacaaatcgt tttttacgat 1500 gttgcagacg ccgagtcatt gattgatgaa ctacaagtaa gagaaacgga aatttttcaa 1560 caagcagagt taagttatcc atctatcgtt aaacgcggcg gcggcttaag agatttgcaa 1620 tatcgtgctt ttgatgaatc atttgtatct gtcgactttt tagtagatgt taaggatgca 1680 atgggggcaa atatcgttaa cgctatgttg gaaggtgtgg ccgagttgtt ccgtgaatgg 1740 tttgcggagc aaaagatttt attcagtatt ttaagtaatt atgccacgga gtcggttgtt 1800 acgatgaaaa cggctattcc agtttcacgt ttaagtaagg ggagcaatgg ccgggaaatt 1860 gctgaaaaaa ttgttttagc ttcacgctat gcttcattag atccttatcg ggcagtcacg 1920 cataacaaag ggatcatgaa tggcattgaa gctgtcgttt tagctacagg aaatgataca 1980 cgcgctgtta gcgcttcttg tcatgctttt gcggtgaagg aaggtcgcta ccaaggtttg 2040 actagttgga cgctggatgg cgaacaacta attggtgaaa tttcagttcc gcttgcgtta 2100 gccacggttg gcggtgccac aaaagtctta cctaaatctc aagcagctgc tgatttgtta 2160 gcagtgacgg atgcaaaaga actaagtcga gtagtagcgg ctgttggttt ggcacaaaat 2220 ttagcggcgt tacgggcctt agtctctgaa ggaattcaaa aaggacacat ggctctacaa 2280 gcacgttctt tagcgatgac ggtcggagct actggtaaag aagttgaggc agtcgctcaa 2340 caattaaaac gtcaaaaaac gatgaaccaa gaccgagcct tggctatttt aaatgattta 2400 agaaaacaat aa 2412 <210> 105 <211> 1149 <212> DNA <213> Enterococcus faecalis mvaS <400> 105 atgacaattg ggattgataa aattagtttt tttgtgcccc cttattatat tgatatgacg 60 gcactggctg aagccagaaa tgtagaccct ggaaaatttc atattggtat tgggcaagac 120 caaatggcgg tgaacccaat cagccaagat attgtgacat ttgcagccaa tgccgcagaa 180 gcgatcttga ccaaagaaga taaagaggcc attgatatgg tgattgtcgg gactgagtcc 240 agtatcgatg agtcaaaagc ggccgcagtt gtcttacatc gtttaatggg gattcaacct 300 ttcgctcgct ctttcgaaat caaggaagct tgttacggag caacagcagg cttacagtta 360 gctaagaatc acgtagcctt acatccagat aaaaaagtct tggttgtagc agcagatatt 420 gcaaaatatg gattaaattc tggcggtgag cctacacaag gagctggggc ggttgcaatg 480 ttagttgcta gtgaaccgcg catcttggct ttaaaagagg ataatgtgat gctgacgcaa 540 gatatctatg acttttggcg tccaacaggc catccgtatc ctatggtcga tggtcctttg 600 tcaaacgaaa cctacatcca atcttttgcc caagtctggg atgaacataa aaaaagaacc 660 ggtcttgatt ttgcagatta tgatgcttta gcgttccata ttccttacac aaaaatgggc 720 aaaaaagcct tattagcaaa aatctccgac caaactgaag cagaacagga acgaatttta 780 gcccgttatg aagaaagcat catctatagt cgtcgcgtag gaaacttgta tacgggttca 840 ctttatctgg gactcatttc ccttttagaa aatgcaacga ctttaaccgc aggcaatcaa 900 attgggttat tcagttatgg ttctggtgct gtcgctgaat ttttcactgg tgaattagta 960 gctggttatc aaaatcattt acaaaaagaa actcatttag cactgctaga taatcggaca 1020 gaactttcta tcgctgaata tgaagccatg tttgcagaaa ctttagacac agatattgat 1080 caaacgttag aagatgaatt aaaatatagt atttctgcta ttaataatac cgttcgctct 1140 tatcgaaac 1149 <210> 106 <211> 879 <212> DNA <213> Streptococcus pneumoniae mvaK1 <400> 106 atgacaaaaa aagttggtgt cggtcaggca catagtaaga taattttaat aggggaacat 60 gcggtcgttt acggttatcc tgccatttcc ctgcctcttt tggaggtgga ggtgacctgt 120 aaggtagttc ctgcagagag tccttggcgc ctttatgagg aggatacctt gtccatggcg 180 gtttatgcct cactggagta tttgaatatc acagaagcct gcattcgttg tgagattgac 240 tcggctatcc ctgagaaacg ggggatgggt tcgtcagcgg ctatcagcat agcggccatt 300 cgtgcagtat ttgactacta tcaggctgat ctgcctcatg atgtactaga aatcttggtc 360 aatcgagctg aaatgattgc ccatatgaat cctagtggtt tggatgctaa gacctgtctt 420 agtgaccaac ctattcgctt tatcaagaac gtaggattta cagaacttga gatggattta 480 tccgcctatt tggtgattgc cgatacgggt gtttatggtc atactcgtga agccatccaa 540 gtggttcaaa ataagggcaa ggatgcccta ccgtttttgc atgccttggg agaattaacc 600 cagcaagcag aagttgcgat ttcacaaaaa gatgctgaag gactgggaca aatcctcagt 660 caagcgcatt tacatttaaa agaaattgga gtcagtagcc ctgaggcaga ctttttggtt 720 gaaacgactc ttagccatgg tgctctgggt gccaagatga gcggtggtgg gctaggaggt 780 tgtatcatag ccttggtaac caatttgaca cacgcacaag aactagcaga aagattagaa 840 gagaaaggag ctgttcagac atggatagag agcctgtaa 879 <210> 107 <211> 1011 <212> DNA <213> Streptococcus pneumoniae mvaK2 <400> 107 atgattgctg ttaaaacttg cggaaaactc tattgggcag gtgaatatgc tattttagag 60 ccagggcagt tagctttgat aaaggatatt cccatctata tgagggctga gattgctttt 120 tctgacagct accgtatcta ttcagatatg tttgatttcg cagtggactt aaggcccaat 180 cctgactaca gcttgattca agaaacgatt gctttgatgg gagacttcct cgctgttcgc 240 ggtcagaatt taagaccttt ttccctaaaa atctgtggca aaatggaacg agaagggaaa 300 aagtttggtc taggttctag tggcagcgtc gttgtcttgg ttgtcaaggc tttactggct 360 ctctataatc tttcggttga tcagaatctc ttgttcaagc tgactagcgc tgtcttgctc 420 aagcgaggag acaatggttc catgggcgac cttgcctgta ttgtggcaga ggatttggtt 480 ctttaccagt catttgatcg ccagaaggcg gctgcttggt tagaagaaga aaacttggcg 540 acagttctgg agcgtgattg gggatttttt atctcacaag tgaaaccaac tttagaatgt 600 gatttcttag tgggatggac caaggaagtg gctgtatcga gtcacatggt ccagcaaatc 660 aagcaaaata tcaatcaaaa ttttttaagt tcctcaaaag aaacggtggt ttctttggtc 720 gaagccttgg agcaggggaa agccgaaaaa gttatcgagc aagtagaagt agccagcaag 780 cttttagaag gcttgagtac agatatttac acgcctttgc ttagacagtt gaaagaagcc 840 agtcaagatt tgcaggccgt tgccaagagt agtggtgctg gtggtggtga ctgtggcatc 900 gccctgagtt ttgatgcgca atcttctcga aacactttaa aaaatcgttg ggccgatctg 960 gggattgagc tcttatatca agaaaggata ggacatgacg acaaatcgta a 1011 <210> 108 <211> 954 <212> DNA <213> Streptococcus pneumoniae mvaD <400> 108 atggatagag agcctgtaac agtacgttcc tacgcaaata ttgctattat caaatattgg 60 ggaaagaaaa aagaaaaaga gatggtgcct gctactagca gtatttctct aactttggaa 120 aatatgtata cagagacgac cttgtcgcct ttaccagcca atgtaacagc tgacgaattt 180 tacatcaatg gtcagctaca aaatgaggtc gagcatgcca agatgagtaa gattattgac 240 cgttatcgtc cagctggtga gggctttgtc cgtatcgata ctcaaaacaa tatgcctacg 300 gcagcgggcc tgtcctcaag ttctagtggt ttgtccgccc tggtcaaggc ttgtaatgct 360 tatttcaagc ttggattgga tagaagtcag ttggcacagg aagccaaatt tgcctcaggc 420 tcttcttctc ggagttttta tggaccacta ggagcctggg ataaggatag tggagaaatt 480 taccctgtag agacagactt gaaactagct atgattatgt tggtgctaga ggacaagaaa 540 aaaccaatct ctagccgtga cgggatgaaa ctttgtgtgg aaacctcgac gacttttgac 600 gactgggttc gtcagtctga gaaggactat caggatatgc tgatttatct caaggaaaat 660 gattttgcca agattggaga attaacggag aaaaatgctc tggctatgca tgctacgaca 720 aagactgcta gtccagcctt ttcttatctg acggatgcct cttatgaggc tatggccttt 780 gttcgccagc ttcgtgagaa aggagaggcc tgctacttta ccatggatgc tggtcccaat 840 gttaaggtct tctgtcagga gaaagacttg gagcatttgt cagaaatttt cggtcagcgt 900 tatcgcttga ttgtgtcaaa aacaaaggat ttgagtcaag atgattgctg ttaa 954 <210> 109 <211> 549 <212> DNA <213> Escherichia coli idi <400> 109 atgcaaacgg aacacgtcat tttattgaat gcacagggag ttcccacggg tacgctggaa 60 aagtatgccg cacacacggc agacacccgc ttacatctcg cgttctccag ttggctgttt 120 aatgccaaag gacaattatt agttacccgc cgcgcactga gcaaaaaagc atggcctggc 180 gtgtggacta actcggtttg tgggcaccca caactgggag aaagcaacga agacgcagtg 240 atccgccgtt gccgttatga gcttggcgtg gaaattacgc ctcctgaatc tatctatcct 300 gactttcgct accgcgccac cgatccgagt ggcattgtgg aaaatgaagt gtgtccggta 360 tttgccgcac gcaccactag tgcgttacag atcaatgatg atgaagtgat ggattatcaa 420 tggtgtgatt tagcagatgt attacacggt attgatgcca cgccgtgggc gttcagtccg 480 tggatggtga tgcaggcgac aaatcgcgaa gccagaaaac gattatctgc atttacccag 540 cttaaataa 549 <210> 110 <211> 915 <212> DNA <213> Haematococcus pluvialis ipiHp1 <400> 110 atgcttcgtt cgttgctcag aggcctcacg catatccccc gcgtgaactc cgcccagcag 60 cccagctgtg cacacgcgcg actccagttt aagctcagga gcatgcagat gacgctcatg 120 cagcccagca tctcagccaa tctgtcgcgc gccgaggacc gcacagacca catgaggggt 180 gcaagcacct gggcaggcgg gcagtcgcag gatgagctga tgctgaagga cgagtgcatc 240 ttggtggatg ttgaggacaa catcacaggc catgccagca agctggagtg tcacaagttc 300 ctaccacatc agcctgcagg cctgctgcac cgggccttct ctgtgttcct gtttgacgat 360 caggggcgac tgctgctgca acagcgtgca cgctcaaaaa tcaccttccc aagtgtgtgg 420 acgaacacct gctgcagcca ccctttacat gggcagaccc cagatgaggt ggaccaacta 480 agccaggtgg ccgacggaac agtacctggc gcaaaggctg ctgccatccg caagttggag 540 cacgagctgg ggataccagc gcaccagctg ccggcaagcg cgtttcgctt cctcacgcgt 600 ttgcactact gtgccgcgga cgtgcagcca gctgcgacac aatcagcgct ctggggcgag 660 cacgaaatgg actacatctt gttcatccgg gccaacgtca ccttggcgcc caaccctgac 720 gaggtggacg aagtcaggta cgtgacgcaa gaggagctgc ggcagatgat gcagccggac 780 aacgggctgc aatggtcgcc gtggtttcgc atcatcgccg cgcgcttcct tgagcgttgg 840 tgggctgacc tggacgcggc cctaaacact gacaaacacg aggattgggg aacggtgcat 900 cacatcaacg aagcg 915 <210> 111 <211> 924 <212> DNA <213> pantoea agglomerans crtE <400> 111 atggtgagtg gcagtaaagc gggcgtttcg cctcatcgcg aaatagaagt aatgagacaa 60 tccattgacg atcacctggc tggcctgtta cctgaaaccg acagccagga tatcgtcagc 120 cttgcgatgc gtgaaggcgt catggcaccc ggtaaacgga tccgtccgct gctgatgctg 180 ctggccgccc gcgacctccg ctaccagggc agtatgccta cgctgctcga tctcgcctgc 240 gccgttgaac tgacccatac cgcgtcgctg atgctcgacg acatgccctg catggacaac 300 gccgagctgc gccgcggtca gcccactacc cacaaaaaat ttggtgagag cgtggcgatc 360 cttgcctccg ttgggctgct ctctaaagcc tttggtctga tcgccgccac cggcgatctg 420 ccgggggaga ggcgtgccca ggcggtcaac gagctctcta ccgccgtggg cgtgcagggc 480 ctggtactgg ggcagtttcg cgatcttaac gatgccgccc tcgaccgtac ccctgacgct 540 atcctcagca ccaaccacct caagaccggc attctgttca gcgcgatgct gcagatcgtc 600 gccattgctt ccgcctcgtc gccgagcacg cgagagacgc tgcacgcctt cgccctcgac 660 ttcggccagg cgtttcaact gctggacgat ctgcgtgacg atcacccgga aaccggtaaa 720 gatcgcaata aggacgcggg aaaatcgacg ctggtcaacc ggctgggcgc agacgcggcc 780 cggcaaaagc tgcgcgagca tattgattcc gccgacaaac acctcacttt tgcctgtccg 840 cagggcggcg ccatccgaca gtttatgcat ctgtggtttg gccatcacct tgccgactgg 900 tcaccggtca tgaaaatcgc ctga 924 <210> 112 <211> 930 <212> DNA <213> pantoea agglomerans crtB <400> 112 atgagccaac cgccgctgct tgaccacgcc acgcagacca tggccaacgg ctcgaaaagt 60 tttgccaccg ctgcgaagct gttcgacccg gccacccgcc gtagcgtgct gatgctctac 120 acctggtgcc gccactgcga tgacgtcatt gacgaccaga cccacggctt cgccagcgag 180 gccgcggcgg aggaggaggc cacccagcgc ctggcccggc tgcgcacgct gaccctggcg 240 gcgtttgaag gggccgagat gcaggatccg gccttcgctg cctttcagga ggtggcgctg 300 acccacggta ttacgccccg catggcgctc gatcacctcg acggctttgc gatggacgtg 360 gctcagaccc gctatgtcac ctttgaggat acgctgcgct actgctatca cgtggcgggc 420 gtggtgggtc tgatgatggc cagggtgatg ggcgtgcggg atgagcgggt gctggatcgc 480 gcctgcgatc tggggctggc cttccagctg acgaatatcg cccgggatat tattgacgat 540 gcggctattg accgctgcta tctgcccgcc gagtggctgc aggatgccgg gctgaccccg 600 gagaactatg ccgcgcggga gaatcgggcc gcgctggcgc gggtggcgga gcggcttatt 660 gatgccgcag agccgtacta catctcctcc caggccgggc tacacgatct gccgccgcgc 720 tgcgcctggg cgatcgccac cgcccgcagc gtctaccggg agatcggtat taaggtaaaa 780 gcggcgggag gcagcgcctg ggatcgccgc cagcacacca gcaaaggtga aaaaattgcc 840 atgctgatgg cggcaccggg gcaggttatt cgggcgaaga cgacgagggt gacgccgcgt 900 ccggccggtc tttggcagcg tcccgtttag 930 <210> 113 <211> 1479 <212> DNA <213> pantoea agglomerans crtI <400> 113 atgaaaaaaa ccgttgtgat tggcgcaggc tttggtggcc tggcgctggc gattcgcctg 60 caggcggcag ggatcccaac cgtactgctg gagcagcggg acaagcccgg cggtcgggcc 120 tacgtctggc atgaccaggg ctttaccttt gacgccgggc cgacggtgat caccgatcct 180 accgcgcttg aggcgctgtt caccctggcc ggcaggcgca tggaggatta cgtcaggctg 240 ctgccggtaa aacccttcta ccgactctgc tgggagtccg ggaagaccct cgactatgct 300 aacgacagcg ccgagcttga ggcgcagatt acccagttca acccccgcga cgtcgagggc 360 taccggcgct ttctggctta ctcccaggcg gtattccagg agggatattt gcgcctcggc 420 agcgtgccgt tcctctcttt tcgcgacatg ctgcgcgccg ggccgcagct gcttaagctc 480 caggcgtggc agagcgtcta ccagtcggtt tcgcgcttta ttgaggatga gcatctgcgg 540 caggccttct cgttccactc cctgctggta ggcggcaacc ccttcaccac ctcgtccatc 600 tacaccctga tccacgccct tgagcgggag tggggggtct ggttccctga gggcggcacc 660 ggggcgctgg tgaacggcat ggtgaagctg tttaccgatc tgggcgggga gatcgaactc 720 aacgcccggg tcgaagagct ggtggtggcc gataaccgcg taagccaggt ccggctggcg 780 gatggtcgga tctttgacac cgacgccgta gcctcgaacg ctgacgtggt gaacacctat 840 aaaaagctgc tcggccacca tccggtgggg cagaagcggg cggcagcgct ggagcgcaag 900 agcatgagca actcgctgtt tgtgctctac ttcggcctga accagcctca ttcccagctg 960 gcgcaccata ccatctgttt tggtccccgc taccgggagc tgatcgacga gatctttacc 1020 ggcagcgcgc tggcggatga cttctcgctc tacctgcact cgccctgcgt gaccgatccc 1080 tcgctcgcgc ctcccggctg cgccagcttc tacgtgctgg ccccggtgcc gcatcttggc 1140 aacgcgccgc tggactgggc gcaggagggg ccgaagctgc gcgaccgcat ctttgactac 1200 cttgaagagc gctatatgcc cggcctgcgt agccagctgg tgacccagcg gatctttacc 1260 ccggcagact tccacgacac gctggatgcg catctgggat cggccttctc catcgagccg 1320 ctgctgaccc aaagcgcctg gttccgcccg cacaaccgcg acagcgacat tgccaacctc 1380 tacctggtgg gcgcaggtac tcaccctggg gcgggcattc ctggcgtagt ggcctcggcg 1440 aaagccaccg ccagcctgat gattgaggat ctgcaatga 1479 <210> 114 <211> 1146 <212> DNA <213> pantoea ananatis crtY <400> 114 atgcaaccgc attatgatct gattctcgtg ggggctggac tcgcgaatgg ccttatcgcc 60 ctgcgtcttc agcagcagca acctgatatg cgtattttgc ttatcgacgc cgcaccccag 120 gcgggcggga atcatacgtg gtcatttcac cacgatgatt tgactgagag ccaacatcgt 180 tggatagctc cgctggtggt tcatcactgg cccgactatc aggtacgctt tcccacacgc 240 cgtcgtaagc tgaacagcgg ctacttttgt attacttctc agcgtttcgc tgaggtttta 300 cagcgacagt ttggcccgca cttgtggatg gataccgcgg tcgcagaggt taatgcggaa 360 tctgttcggt tgaaaaaggg tcaggttatc ggtgcccgcg cggtgattga cgggcggggt 420 tatgcggcaa attcagcact gagcgtgggc ttccaggcgt ttattggcca ggaatggcga 480 ttgagccacc cgcatggttt atcgtctccc attatcatgg atgccacggt cgatcagcaa 540 aatggttatc gcttcgtgta cagcctgccg ctctcgccga ccagattgtt aattgaagac 600 acgcactata ttgataatgc gacattagat cctgaatgcg cgcggcaaaa tatttgcgac 660 tatgccgcgc aacagggttg gcagcttcag acactgctgc gagaagaaca gggcgcctta 720 cccattactc tgtcgggcaa tgccgacgca ttctggcagc agcgccccct ggcctgtagt 780 ggattacgtg ccggtctgtt ccatcctacc accggctatt cactgccgct ggcggttgcc 840 gtggccgacc gcctgagtgc acttgatgtc tttacgtcgg cctcaattca ccatgccatt 900 acgcattttg cccgcgagcg ctggcagcag cagggctttt tccgcatgct gaatcgcatg 960 ctgtttttag ccggacccgc cgattcacgc tggcgggtta tgcagcgttt ttatggttta 1020 cctgaagatt taattgcccg tttttatgcg ggaaaactca cgctgaccga tcggctacgt 1080 attctgagcg gcaagccgcc tgttccggta ttagcagcat tgcaagccat tatgacgact 1140 catcgt 1146 <210> 115 <211> 828 <212> DNA <213> uncultured marine bacterium 66A03 SR <400> 115 atgggtctga tgctgattga ttggtgtgca ctggctctgg ttgttttcat tggcctgccg 60 cacggcgcgc tggatgctgc catttctttt tctatgatct cttctgcaaa acgcattgct 120 cgtctggctg gtattctgct gatctatctg ctgctggcga ccgcgttctt cctgatctgg 180 tatcagctgc cagcgtttag cctgctgatc ttcctgctga tctccattat ccactttggt 240 atggcagact tcaacgcgtc cccaagcaaa ctgaaatggc cgcatatcat cgcccacggc 300 ggtgttgtta ctgtttggct gccgctgatc cagaaaaacg aagtaactaa actgtttagc 360 atcctgacta acggtccgac tccgatcctg tgggacatcc tgctgatttt cttcctgtgt 420 tggtctattg gcgtgtgtct gcacacgtac gaaaccctgc gctctaaaca ttacaacatc 480 gcctttgaac tgatcggtct gattttcctg gcgtggtatg cgccgcctct ggttacgttt 540 gccacttact tctgcttcat tcattcccgt cgccacttct cctttgtgtg gaagcagctg 600 caacacatgt cttccaaaaa gatgatgatt ggcagcgcga ttatcctgtc ctgtacctct 660 tggctgatcg gcggtggtat ctatttcttc ctgaactcca aaatgatcgc ctctgaggct 720 gcgctgcaga ctgtgttcat cggtctggcg gcactgaccg tgccgcacat gattctgatc 780 gacttcatct tccgtccgca ctcttcccgt atcaaaatca aaaactaa 828 <210> 116 <211> 810 <212> DNA <213> Escherichia coli MG1655 YbbO <400> 116 atgactcata aagcaacgga gatcctgaca ggtaaagtta tgcaaaaatc ggtcttaatt 60 accggatgtt ccagtggaat tggcctggaa agcgcgctcg aattaaaacg ccagggtttt 120 catgtgctgg caggttgccg gaaaccggat gatgttgagc gcatgaacag catgggattt 180 accggcgtgt tgatcgatct ggattcacca gaaagtgttg atcgcgcagc cgacgaggtg 240 atcgccctga ccgataattg tctgtatggg atctttaaca atgccggatt cggcatgtat 300 ggcccccttt ccaccatcag ccgtgcgcag atggaacagc agttttccgc caactttttc 360 ggcgcacacc agctcaccat gcgcctgtta cccgcgatgt taccgcacgg tgaagggcgt 420 attgtgatga catcatcggt gatgggatta atctccacgc cgggtcgtgg cgcttacgcg 480 gccagtaaat atgcgctgga ggcgtggtca gatgcactgc gcatggagct gcgccacagc 540 ggaattaaag tcagcctgat cgaacccggt cccattcgta ctcgcttcac cgacaacgtc 600 aaccagacgc aaagtgataa accagtcgaa aatcccggca tcgccgcccg ctttacgttg 660 ggaccggaag cggtggtgga caaagtacgc catgctttta ttagcgagaa gccgaagatg 720 cgctatccgg tgacgctggt gacctgggcg gtaatggtgc ttaagcgcct gctgccgggg 780 cgcgtgatgg acaaaatatt gcaggggtaa 810 <210> 117 <211> 1863 <212> DNA <213> Escherichia coli dxs <400> 117 atgagttttg atattgccaa atacccgacc ctggcactgg tcgactccac ccaggagtta 60 cgactgttgc cgaaagagag tttaccgaaa ctctgcgacg aactgcgccg ctatttactc 120 gacagcgtga gccgttccag cgggcacttc gcctccgggc tgggcacggt cgaactgacc 180 gtggcgctgc actatgtcta caacaccccg tttgaccaat tgatttggga tgtggggcat 240 caggcttatc cgcataaaat tttgaccgga cgccgcgaca aaatcggcac catccgtcag 300 aaaggcggtc tgcacccgtt cccgtggcgc ggcgaaagcg aatatgacgt attaagcgtc 360 gggcattcat caacctccat cagtgccgga attggtattg cggttgctgc cgaaaaagaa 420 ggcaaaaatc gccgcaccgt ctgtgtcatt ggcgatggcg cgattaccgc aggcatggcg 480 tttgaagcga tgaatcacgc gggcgatatc cgtcctgata tgctggtgat tctcaacgac 540 aatgaaatgt cgatttccga aaatgtcggc gcgctcaaca accatctggc acagctgctt 600 tccggtaagc tttactcttc actgcgcgaa ggcgggaaaa aagttttctc tggcgtgccg 660 ccaattaaag agctgctcaa acgcaccgaa gaacatatta aaggcatggt agtgcctggc 720 acgttgtttg aagagctggg ctttaactac atcggcccgg tggacggtca cgatgtgctg 780 gggcttatca ccacgctaaa gaacatgcgc gacctgaaag gcccgcagtt cctgcatatc 840 atgaccaaaa aaggtcgtgg ttatgaaccg gcagaaaaag acccgatcac tttccacgcc 900 gtgcctaaat ttgatccctc cagcggttgt ttgccgaaaa gtagcggcgg tttgccgagc 960 tattcaaaaa tctttggcga ctggttgtgc gaaacggcag cgaaagacaa caagctgatg 1020 gcgattactc cggcgatgcg tgaaggttcc ggcatggtcg agttttcacg taaattcccg 1080 gatcgctact tcgacgtggc aattgccgag caacacgcgg tgacctttgc tgcgggtctg 1140 gcgattggtg ggtacaaacc cattgtcgcg atttactcca ctttcctgca acgcgcctat 1200 gatcaggtgc tgcatgacgt ggcgattcaa aagcttccgg tcctgttcgc catcgaccgc 1260 gcgggcattg ttggtgctga cggtcaaacc catcagggtg cttttgatct ctcttacctg 1320 cgctgcatac cggaaatggt cattatgacc ccgagcgatg aaaacgaatg tcgccagatg 1380 ctctataccg gctatcacta taacgatggc ccgtcagcgg tgcgctaccc gcgtggcaac 1440 gcggtcggcg tggaactgac gccgctggaa aaactaccaa ttggcaaagg cattgtgaag 1500 cgtcgtggcg agaaactggc gatccttaac tttggtacgc tgatgccaga agcggcgaaa 1560 gtcgccgaat cgctgaacgc cacgctggtc gatatgcgtt ttgtgaaacc gcttgatgaa 1620 gcgttaattc tggaaatggc cgccagccat gaagcgctgg tcaccgtaga agaaaacgcc 1680 attatgggcg gcgcaggcag cggcgtgaac gaagtgctga tggcccatcg taaaccagta 1740 cccgtgctga acattggcct gccggacttc tttattccgc aaggaactca ggaagaaatg 1800 cgcgccgaac tcggcctcga tgccgctggt atggaagcca aaatcaaggc ctggctggca 1860 taa 1863 <210> 118 <211> 1197 <212> DNA <213> Escherichia coli dxr <400> 118 atgaagcaac tcaccattct gggctcgacc ggctcgattg gttgcagcac gctggacgtg 60 gtgcgccata atcccgaaca cttccgcgta gttgcgctgg tggcaggcaa aaatgtcact 120 cgcatggtag aacagtgcct ggaattctct ccccgctatg ccgtaatgga cgatgaagcg 180 agtgcgaaac ttcttaaaac gatgctacag caacagggta gccgcaccga agtcttaagt 240 gggcaacaag ccgcttgcga tatggcagcg cttgaggatg ttgatcaggt gatggcagcc 300 attgttggcg ctgctgggct gttacctacg cttgctgcga tccgcgcggg taaaaccatt 360 ttgctggcca ataaagaatc actggttacc tgcggacgtc tgtttatgga cgccgtaaag 420 cagagcaaag cgcaattgtt accggtcgat agcgaacata acgccatttt tcagagttta 480 ccgcaaccta tccagcataa tctgggatac gctgaccttg agcaaaatgg cgtggtgtcc 540 attttactta ccgggtctgg tggccctttc cgtgagacgc cattgcgcga tttggcaaca 600 atgacgccgg atcaagcctg ccgtcatccg aactggtcga tggggcgtaa aatttctgtc 660 gattcggcta ccatgatgaa caaaggtctg gaatacattg aagcgcgttg gctgtttaac 720 gccagcgcca gccagatgga agtgctgatt cacccgcagt cagtgattca ctcaatggtg 780 cgctatcagg acggcagtgt tctggcgcag ctgggggaac cggatatgcg tacgccaatt 840 gcccacacca tggcatggcc gaatcgcgtg aactctggcg tgaagccgct cgatttttgc 900 aaactaagtg cgttgacatt tgccgcaccg gattatgatc gttatccatg cctgaaactg 960 gcgatggagg cgttcgaaca aggccaggca gcgacgacag cattgaatgc cgcaaacgaa 1020 atcaccgttg ctgcttttct tgcgcaacaa atccgcttta cggatatcgc tgcgttgaat 1080 ttatccgtac tggaaaaaat ggatatgcgc gaaccacaat gtgtggacga tgtgttatct 1140 gttgatgcga acgcgcgtga agtcgccaga aaagaggtga tgcgtctcgc aagctga 1197 <210> 119 <211> 900 <212> DNA <213> Escherichia coli ispA <400> 119 atggactttc cgcagcaact cgaagcctgc gttaagcagg ccaaccaggc gctgagccgt 60 tttatcgccc cactgccctt tcagaacact cccgtggtcg aaaccatgca gtatggcgca 120 ttattaggtg gtaagcgcct gcgacctttc ctggtttatg ccaccggtca tatgttcggc 180 gttagcacaa acacgctgga cgcacccgct gccgccgttg agtgtatcca cgcttactca 240 ttaattcatg atgatttacc ggcaatggat gatgacgatc tgcgtcgcgg tttgccaacc 300 tgccatgtga agtttggcga agcaaacgcg attctcgctg gcgacgcttt acaaacgctg 360 gcgttctcga ttttaagcga tgccgatatg ccggaagtgt cggaccgcga cagaatttcg 420 atgatttctg aactggcgag cgccagtggt attgccggaa tgtgcggtgg tcaggcatta 480 gatttagacg cggaaggcaa acacgtacct ctggacgcgc ttgagcgtat tcatcgtcat 540 aaaaccggcg cattgattcg cgccgccgtt cgccttggtg cattaagcgc cggagataaa 600 ggacgtcgtg ctctgccggt actcgacaag tatgcagaga gcatcggcct tgccttccag 660 gttcaggatg acatcctgga tgtggtggga gatactgcaa cgttgggaaa acgccagggt 720 gccgaccagc aacttggtaa aagtacctac cctgcacttc tgggtcttga gcaagcccgg 780 aagaaagccc gggatctgat cgacgatgcc cgtcagtcgc tgaaacaact ggctgaacag 840 tcactcgata cctcggcact ggaagcgcta gcggactaca tcatccagcg taataaataa 900 900 <210> 120 <211> 1656 <212> DNA <213> Clausena lansium STS <400> 120 atgtcaactc aacaagtttc atcagagaac attgttcgta acgctgcgaa tttccatcct 60 aatatatggg gaaaccattt cctcacatgt ccttctcaga cgattgatag ttggactcaa 120 cagcaccaca aagaactgaa agaagaggtg aggaaaatga tggtgtctga tgcaaataaa 180 cctgcccaga gattgcgctt gattgatact gtccaaaggc taggtgtggc ttaccacttt 240 gaaaaggaga ttgatgatgc attggagaaa ataggtcatg acccttttga tgataaagat 300 gatctctaca ttgtctctct ttgttttcga ttgctgaggc agcatggaat taagatatca 360 tgtgatgtgt ttgagaagtt taaagatgac gatggaaaat tcaaggcatc attgatgaat 420 gatgttcaag gcatgctaag tttatatgag gcagcacacc tagccattca cggagaagat 480 attttagatg aagcaattgt tttcacgacc actcacctta agtcaacggt atctaattct 540 cctgtaaact ctacttttgc tgaacaaata cgtcattctc tcagagttcc tctccgtaaa 600 gctgtaccta ggttagagtc gaggtatttc ttggatatct attcaagaga tgatttgcac 660 gataaaactt tgctcaattt cgcaaagtta gactttaata tactacaagc aatgcaccag 720 aaggaagcaa gtgagatgac caggtggtgg agagattttg acttccttaa aaagctgcct 780 tatataagag acagagtcgt ggagctatat ttttggattc tggtgggagt gtcttatcag 840 cccaaattca gcactggtag aatttttttg tccaaaataa tatgccttga gaccctcgta 900 gatgatacat ttgacgccta cggtactttt gacgagctcg caatctttac tgaagcagtt 960 acaagatggg accttggcca cagagatgca ctaccagaat acatgaaatt cattttcaag 1020 acactcattg atgtctacag tgaagctgag caagaactgg caaaggaagg gagatcatac 1080 agcatacact atgcaatacg atcgttccaa gaactagtta tgaagtactt ctgcgaagcc 1140 aagtggttaa ataaaggtta tgttccgagc ctggacgatt ataaatcagt ttcattaaga 1200 agtatcggtt ttttaccgat agcggtagct tccttcgttt tcatgggtga tattgcaact 1260 aaggaggtct ttgaatggga aatgaataac cctaagatca taatagccgc agaaacgatt 1320 ttcagattcc tggatgacat agcaggccat aggtttgagc aaaagagaga acatagtcca 1380 tcagctattg aatgctacaa gaatcaacat ggagtgtctg aggaagaggc agttaaagcg 1440 ttgtcgttag aagttgctaa tagttggaaa gatataaatg aggagctgct tctcaaccca 1500 atggctattc ctttacctct gcttcaggtg attcttgatc tctcacgttc ggccgatttt 1560 atgtacggta atgctcaaga tcgcttcacg cattcaacga tgatgaaaga ccaagttgat 1620 ttggtgctga aggaccccgt taagcttgac gattaa 1656 <210> 121 <211> 1719 <212> DNA <213> Artificial Sequence <220> <223> Matricaria recutita MrBBS <400> 121 atgtcaaccc tgtcagtctc cacgccgtcc ttctcatcgt cgccgctgtc ctcagtgaac 60 aaaaatagca cgaaacaaca cgttacgcgc aatagtgtga tctttcatga ttccatttgg 120 ggcgaccagt tcctggaata caaagaaaaa ttcaacgttg caaccgaaaa acaactgatt 180 gaagaactga aagaagaagt ccgcaatgaa ctgatgatcc gtgcgtgcaa cgaagccagt 240 cgctatatta aactgatcca gctgattgat gtggttgaac gtctgggcct ggcctaccac 300 tttgaaaaag aaatcgaaga atccctgcaa catatttatg ttacctacgg tcacaaatgg 360 acgaactaca acaacatcga aagcctgtct ctgtggtttc gcctgctgcg tcagaacggt 420 tttaatgtca gctctgatat cttcgaaaac catattgacg aaaaaggcaa tttccaagaa 480 agtctgtgca acgatccgca aggcatgctg gcactgtatg aagcggccta catgcgcgtg 540 gaaggcgaaa ttatcctgga caaagctctg gaatttacca aactgcacct gggtattatc 600 agcaacgatc cgtcttgtga cagttccctg cgtacggaaa ttaaacaggc gctgaaacag 660 ccgctgcgtc gccgtctgcc gcgtctggaa gcagttcgtt atattgctat ctaccagcaa 720 aaagcgagtc attccgaagt cctgctgaaa ctggccaaac tggatttcaa tgtgctgcag 780 gaaatgcaca aagacgaact gtcacaaatt tgtaaatggt ggaaagacct ggacatccgt 840 aacaaactgc cgtatgttcg cgatcgtctg attgaaggct atttttggat tctgggtatt 900 tacttcgaac cgcagcattc gcgcacccgt atgtttctga tgaaaacgtg catgtggctg 960 atcgtcctgg atgacacctt tgataattat ggcacgtacg aagaactgga aattttcacc 1020 caggcggtgg aacgctggag catcacgtgt ctggatgaac tgccggaata catgaaactg 1080 atctaccatg aacagttccg tgtgcaccaa gaaatggaag aatctctgga aaaagaaggt 1140 aaagcatacc agatccatta catcaaagaa atggctaaag aaggcacccg ctctctgctg 1200 ctggaagcga aatggctgaa agaaggttat atgccgacgc tggatgaata cctgtcaaac 1260 tcgctggtta cctgcggcta tgcactgatg acggctcgct catacgttgc ccgtgatgac 1320 ggtattgtca ccgaagatgc gtttaaatgg gtggccacgc acccgccgat cgttaaagca 1380 gcttgtaaaa ttctgcgtct gatggatgac atcgcgaccc ataaagaaga acaggaacgc 1440 ggccacatcg cctcatcgat tgaatgctat cgtaaagaaa ccggtgcatc tgaagaagaa 1500 gcgtgtatgg attttctgaa acaggtcgaa gacggctgga aagtgatcaa tcaagaatca 1560 ctgatgccga ccgatgtgcc gttcccgctg ctgatcccgg cgattaacct ggcccgcgtt 1620 tcggacacgc tgtataaaga taacgacggt tacaatcatg ctgataaaga agtgattggt 1680 tacattaaaa gtctgtttgt ccacccgatg attgtgtga 1719 <210> 122 <211> 717 <212> DNA <213> Artificial Sequence <220> <223> EGFP <400> 122 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 60 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac 120 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc 180 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag 240 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 300 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 360 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 420 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac 480 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc 540 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac 600 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc 660 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaag 717 <210> 123 <211> 1233 <212> DNA <213> Artificial Sequence <220> <223> Lactococcus lactis subsp. cremoris MG1363 Hydroxymethylglutaryl-CoA reductase <400> 123 atgagaaaaa aattttatca aatgtcgcca caagagcgat tgaattcgtt aaacttatcc 60 gaaaagagtc aagaaatttt aagtgagatg gccctagata caactatttt ggacaatctc 120 atagaaaatc aaatttctga atttgaattg ccaatgggaa ttgcccaaaa ttttgttatt 180 aatggacaaa gttttctaat accaatggtt acagaggaac cgtcagtaat tgccgcagca 240 agtaatggag caaaaatagc tggaaatttt gtagctgaaa ttaaagaacg tttaatgcgt 300 ggtcaaattg tattttatga tgttaaaaat tcagataaga ttgcaaatga aatacttgaa 360 aagcaagaaa aaatctttga acaagccgaa ctttcttacc cttcaatcgt taaacgaggt 420 ggcggtttga gagaggtttc tagtcggatt ttctctagtc aaaagttttt atctgttgat 480 gtcaaggttg atgttaaaga cgccatgggt gcaaatatta ttaattcaat cttagaggga 540 attgctgaac tctttcggag gtggtttcct gatgaaaaaa ttctgtttag tattttatca 600 aattatgcta ctgaatcact agtgaaagtg acctgtgaaa ttccggttga acgcctttca 660 aaaaaagcag atggatatga aattggtcaa aaaattatgg ctgcttccca atattcaaaa 720 attgatcctt atcgtgctag cacccataac aaagggatta tgaacggtat caatgcggtc 780 attttagcta caggaaatga tactcgggcc atctcagctg ctattcatgc ttatgcggca 840 aaagatggag cctaccaagg gttagccaac tgggaacttc aagagaaaat gttagttggc 900 gaactcgaat ttcctttacc agtggcaact gtcggagggg gagtcaaggt tttgccaaaa 960 gcacaagcgg ccatggagat tctgggtatc agtgatgcta aggagctcgc aaaagttatt 1020 gctgcagtgg gactagctca aaacttagcc gctttacgtg ccctagtttc agaaggaatt 1080 caacagggac acatgagtct acaagctcgt tctttggctt tgagtgtagg agcacaagcg 1140 gatgaaatcg cgatattaag tcaacaattg cgcaaagaaa aagtgatgaa tcaagaagta 1200 gcccaaaatt tactaaaaaa tttgagaaaa taa 1233 <210> 124 <211> 803 <212> PRT <213> Enterococcus faecalis mvaE <400> 124 Leu Lys Thr Val Val Ile Ile Asp Ala Leu Arg Thr Pro Ile Gly Lys 1 5 10 15 Tyr Lys Gly Ser Leu Ser Gln Val Ser Ala Val Asp Leu Gly Thr His 20 25 30 Val Thr Thr Gln Leu Leu Lys Arg His Ser Thr Ile Ser Glu Glu Ile 35 40 45 Asp Gln Val Ile Phe Gly Asn Val Leu Gln Ala Gly Asn Gly Gln Asn 50 55 60 Pro Ala Arg Gln Ile Ala Ile Asn Ser Gly Leu Ser His Glu Ile Pro 65 70 75 80 Ala Met Thr Val Asn Glu Val Cys Gly Ser Gly Met Lys Ala Val Ile 85 90 95 Leu Ala Lys Gln Leu Ile Gln Leu Gly Glu Ala Glu Val Leu Ile Ala 100 105 110 Gly Gly Ile Glu Asn Met Ser Gln Ala Pro Lys Leu Gln Arg Phe Asn 115 120 125 Tyr Glu Thr Glu Ser Tyr Asp Ala Pro Phe Ser Ser Met Met Tyr Asp 130 135 140 Gly Leu Thr Asp Ala Phe Ser Gly Gln Ala Met Gly Leu Thr Ala Glu 145 150 155 160 Asn Val Ala Glu Lys Tyr His Val Thr Arg Glu Glu Gln Asp Gln Phe 165 170 175 Ser Val His Ser Gln Leu Lys Ala Ala Gln Ala Gln Ala Glu Gly Ile 180 185 190 Phe Ala Asp Glu Ile Ala Pro Leu Glu Val Ser Gly Thr Leu Val Glu 195 200 205 Lys Asp Glu Gly Ile Arg Pro Asn Ser Ser Val Glu Lys Leu Gly Thr 210 215 220 Leu Lys Thr Val Phe Lys Glu Asp Gly Thr Val Thr Ala Gly Asn Ala 225 230 235 240 Ser Thr Ile Asn Asp Gly Ala Ser Ala Leu Ile Ile Ala Ser Gln Glu 245 250 255 Tyr Ala Glu Ala His Gly Leu Pro Tyr Leu Ala Ile Ile Arg Asp Ser 260 265 270 Val Glu Val Gly Ile Asp Pro Ala Tyr Met Gly Ile Ser Pro Ile Lys 275 280 285 Ala Ile Gln Lys Leu Leu Ala Arg Asn Gln Leu Thr Thr Glu Glu Ile 290 295 300 Asp Leu Tyr Glu Ile Asn Glu Ala Phe Ala Ala Thr Ser Ile Val Val 305 310 315 320 Gln Arg Glu Leu Ala Leu Pro Glu Glu Lys Val Asn Ile Tyr Gly Gly 325 330 335 Gly Ile Ser Leu Gly His Ala Ile Gly Ala Thr Gly Ala Arg Leu Leu 340 345 350 Thr Ser Leu Ser Tyr Gln Leu Asn Gln Lys Glu Lys Lys Tyr Gly Val 355 360 365 Ala Ser Leu Cys Ile Gly Gly Gly Leu Gly Leu Ala Met Leu Leu Glu 370 375 380 Arg Pro Gln Gln Lys Lys Asn Ser Arg Phe Tyr Gln Met Ser Pro Glu 385 390 395 400 Glu Arg Leu Ala Ser Leu Leu Asn Glu Gly Gln Ile Ser Ala Asp Thr 405 410 415 Lys Lys Glu Phe Glu Asn Thr Ala Leu Ser Ser Gln Ile Ala Asn His 420 425 430 Met Ile Glu Asn Gln Ile Ser Glu Thr Glu Val Pro Met Gly Val Gly 435 440 445 Leu His Leu Thr Val Asp Glu Thr Asp Tyr Leu Val Pro Met Ala Thr 450 455 460 Glu Glu Pro Ser Val Ile Ala Ala Leu Ser Asn Gly Ala Lys Ile Ala 465 470 475 480 Gln Gly Phe Lys Thr Val Asn Gln Gln Arg Leu Met Arg Gly Gln Ile 485 490 495 Val Phe Tyr Asp Val Ala Asp Ala Glu Ser Leu Ile Asp Glu Leu Gln 500 505 510 Val Arg Glu Thr Glu Ile Phe Gln Gln Ala Glu Leu Ser Tyr Pro Ser 515 520 525 Ile Val Lys Arg Gly Gly Gly Leu Arg Asp Leu Gln Tyr Arg Ala Phe 530 535 540 Asp Glu Ser Phe Val Ser Val Asp Phe Leu Val Asp Val Lys Asp Ala 545 550 555 560 Met Gly Ala Asn Ile Val Asn Ala Met Leu Glu Gly Val Ala Glu Leu 565 570 575 Phe Arg Glu Trp Phe Ala Glu Gln Lys Ile Leu Phe Ser Ile Leu Ser 580 585 590 Asn Tyr Ala Thr Glu Ser Val Val Thr Met Lys Thr Ala Ile Pro Val 595 600 605 Ser Arg Leu Ser Lys Gly Ser Asn Gly Arg Glu Ile Ala Glu Lys Ile 610 615 620 Val Leu Ala Ser Arg Tyr Ala Ser Leu Asp Pro Tyr Arg Ala Val Thr 625 630 635 640 His Asn Lys Gly Ile Met Asn Gly Ile Glu Ala Val Val Leu Ala Thr 645 650 655 Gly Asn Asp Thr Arg Ala Val Ser Ala Ser Cys His Ala Phe Ala Val 660 665 670 Lys Glu Gly Arg Tyr Gln Gly Leu Thr Ser Trp Thr Leu Asp Gly Glu 675 680 685 Gln Leu Ile Gly Glu Ile Ser Val Pro Leu Ala Leu Ala Thr Val Gly 690 695 700 Gly Ala Thr Lys Val Leu Pro Lys Ser Gln Ala Ala Ala Asp Leu Leu 705 710 715 720 Ala Val Thr Asp Ala Lys Glu Leu Ser Arg Val Val Ala Ala Val Gly 725 730 735 Leu Ala Gln Asn Leu Ala Ala Leu Arg Ala Leu Val Ser Glu Gly Ile 740 745 750 Gln Lys Gly His Met Ala Leu Gln Ala Arg Ser Leu Ala Met Thr Val 755 760 765 Gly Ala Thr Gly Lys Glu Val Glu Ala Val Ala Gln Gln Leu Lys Arg 770 775 780 Gln Lys Thr Met Asn Gln Asp Arg Ala Leu Ala Ile Leu Asn Asp Leu 785 790 795 800 Arg Lys Gln <210> 125 <211> 383 <212> PRT <213> Enterococcus faecalis mvaS <400> 125 Met Thr Ile Gly Ile Asp Lys Ile Ser Phe Phe Val Pro Pro Tyr Tyr 1 5 10 15 Ile Asp Met Thr Ala Leu Ala Glu Ala Arg Asn Val Asp Pro Gly Lys 20 25 30 Phe His Ile Gly Ile Gly Gln Asp Gln Met Ala Val Asn Pro Ile Ser 35 40 45 Gln Asp Ile Val Thr Phe Ala Ala Asn Ala Ala Glu Ala Ile Leu Thr 50 55 60 Lys Glu Asp Lys Glu Ala Ile Asp Met Val Ile Val Gly Thr Glu Ser 65 70 75 80 Ser Ile Asp Glu Ser Lys Ala Ala Ala Val Val Leu His Arg Leu Met 85 90 95 Gly Ile Gln Pro Phe Ala Arg Ser Phe Glu Ile Lys Glu Ala Cys Tyr 100 105 110 Gly Ala Thr Ala Gly Leu Gln Leu Ala Lys Asn His Val Ala Leu His 115 120 125 Pro Asp Lys Lys Val Leu Val Val Ala Ala Asp Ile Ala Lys Tyr Gly 130 135 140 Leu Asn Ser Gly Gly Glu Pro Thr Gln Gly Ala Gly Ala Val Ala Met 145 150 155 160 Leu Val Ala Ser Glu Pro Arg Ile Leu Ala Leu Lys Glu Asp Asn Val 165 170 175 Met Leu Thr Gln Asp Ile Tyr Asp Phe Trp Arg Pro Thr Gly His Pro 180 185 190 Tyr Pro Met Val Asp Gly Pro Leu Ser Asn Glu Thr Tyr Ile Gln Ser 195 200 205 Phe Ala Gln Val Trp Asp Glu His Lys Lys Arg Thr Gly Leu Asp Phe 210 215 220 Ala Asp Tyr Asp Ala Leu Ala Phe His Ile Pro Tyr Thr Lys Met Gly 225 230 235 240 Lys Lys Ala Leu Leu Ala Lys Ile Ser Asp Gln Thr Glu Ala Glu Gln 245 250 255 Glu Arg Ile Leu Ala Arg Tyr Glu Glu Ser Ile Ile Tyr Ser Arg Arg 260 265 270 Val Gly Asn Leu Tyr Thr Gly Ser Leu Tyr Leu Gly Leu Ile Ser Leu 275 280 285 Leu Glu Asn Ala Thr Thr Leu Thr Ala Gly Asn Gln Ile Gly Leu Phe 290 295 300 Ser Tyr Gly Ser Gly Ala Val Ala Glu Phe Phe Thr Gly Glu Leu Val 305 310 315 320 Ala Gly Tyr Gln Asn His Leu Gln Lys Glu Thr His Leu Ala Leu Leu 325 330 335 Asp Asn Arg Thr Glu Leu Ser Ile Ala Glu Tyr Glu Ala Met Phe Ala 340 345 350 Glu Thr Leu Asp Thr Asp Ile Asp Gln Thr Leu Glu Asp Glu Leu Lys 355 360 365 Tyr Ser Ile Ser Ala Ile Asn Asn Thr Val Arg Ser Tyr Arg Asn 370 375 380 <210> 126 <211> 292 <212> PRT <213> Streptococcus pneumoniae mvaK1 <400> 126 Met Thr Lys Lys Val Gly Val Gly Gln Ala His Ser Lys Ile Ile Leu 1 5 10 15 Ile Gly Glu His Ala Val Val Tyr Gly Tyr Pro Ala Ile Ser Leu Pro 20 25 30 Leu Leu Glu Val Glu Val Thr Cys Lys Val Val Pro Ala Glu Ser Pro 35 40 45 Trp Arg Leu Tyr Glu Glu Asp Thr Leu Ser Met Ala Val Tyr Ala Ser 50 55 60 Leu Glu Tyr Leu Asn Ile Thr Glu Ala Cys Ile Arg Cys Glu Ile Asp 65 70 75 80 Ser Ala Ile Pro Glu Lys Arg Gly Met Gly Ser Ser Ala Ala Ile Ser 85 90 95 Ile Ala Ala Ile Arg Ala Val Phe Asp Tyr Tyr Gln Ala Asp Leu Pro 100 105 110 His Asp Val Leu Glu Ile Leu Val Asn Arg Ala Glu Met Ile Ala His 115 120 125 Met Asn Pro Ser Gly Leu Asp Ala Lys Thr Cys Leu Ser Asp Gln Pro 130 135 140 Ile Arg Phe Ile Lys Asn Val Gly Phe Thr Glu Leu Glu Met Asp Leu 145 150 155 160 Ser Ala Tyr Leu Val Ile Ala Asp Thr Gly Val Tyr Gly His Thr Arg 165 170 175 Glu Ala Ile Gln Val Val Gln Asn Lys Gly Lys Asp Ala Leu Pro Phe 180 185 190 Leu His Ala Leu Gly Glu Leu Thr Gln Gln Ala Glu Val Ala Ile Ser 195 200 205 Gln Lys Asp Ala Glu Gly Leu Gly Gln Ile Leu Ser Gln Ala His Leu 210 215 220 His Leu Lys Glu Ile Gly Val Ser Ser Pro Glu Ala Asp Phe Leu Val 225 230 235 240 Glu Thr Thr Leu Ser His Gly Ala Leu Gly Ala Lys Met Ser Gly Gly 245 250 255 Gly Leu Gly Gly Cys Ile Ile Ala Leu Val Thr Asn Leu Thr His Ala 260 265 270 Gln Glu Leu Ala Glu Arg Leu Glu Glu Lys Gly Ala Val Gln Thr Trp 275 280 285 Ile Glu Ser Leu 290 <210> 127 <211> 336 <212> PRT <213> Streptococcus pneumoniae mvaK2 <400> 127 Met Ile Ala Val Lys Thr Cys Gly Lys Leu Tyr Trp Ala Gly Glu Tyr 1 5 10 15 Ala Ile Leu Glu Pro Gly Gln Leu Ala Leu Ile Lys Asp Ile Pro Ile 20 25 30 Tyr Met Arg Ala Glu Ile Ala Phe Ser Asp Ser Tyr Arg Ile Tyr Ser 35 40 45 Asp Met Phe Asp Phe Ala Val Asp Leu Arg Pro Asn Pro Asp Tyr Ser 50 55 60 Leu Ile Gln Glu Thr Ile Ala Leu Met Gly Asp Phe Leu Ala Val Arg 65 70 75 80 Gly Gln Asn Leu Arg Pro Phe Ser Leu Lys Ile Cys Gly Lys Met Glu 85 90 95 Arg Glu Gly Lys Lys Phe Gly Leu Gly Ser Ser Gly Ser Val Val Val 100 105 110 Leu Val Val Lys Ala Leu Leu Ala Leu Tyr Asn Leu Ser Val Asp Gln 115 120 125 Asn Leu Leu Phe Lys Leu Thr Ser Ala Val Leu Leu Lys Arg Gly Asp 130 135 140 Asn Gly Ser Met Gly Asp Leu Ala Cys Ile Val Ala Glu Asp Leu Val 145 150 155 160 Leu Tyr Gln Ser Phe Asp Arg Gln Lys Ala Ala Ala Trp Leu Glu Glu 165 170 175 Glu Asn Leu Ala Thr Val Leu Glu Arg Asp Trp Gly Phe Phe Ile Ser 180 185 190 Gln Val Lys Pro Thr Leu Glu Cys Asp Phe Leu Val Gly Trp Thr Lys 195 200 205 Glu Val Ala Val Ser Ser His Met Val Gln Gln Ile Lys Gln Asn Ile 210 215 220 Asn Gln Asn Phe Leu Ser Ser Ser Lys Glu Thr Val Val Ser Leu Val 225 230 235 240 Glu Ala Leu Glu Gln Gly Lys Ala Glu Lys Val Ile Glu Gln Val Glu 245 250 255 Val Ala Ser Lys Leu Leu Glu Gly Leu Ser Thr Asp Ile Tyr Thr Pro 260 265 270 Leu Leu Arg Gln Leu Lys Glu Ala Ser Gln Asp Leu Gln Ala Val Ala 275 280 285 Lys Ser Ser Gly Ala Gly Gly Gly Asp Cys Gly Ile Ala Leu Ser Phe 290 295 300 Asp Ala Gln Ser Ser Arg Asn Thr Leu Lys Asn Arg Trp Ala Asp Leu 305 310 315 320 Gly Ile Glu Leu Leu Tyr Gln Glu Arg Ile Gly His Asp Asp Lys Ser 325 330 335 <210> 128 <211> 317 <212> PRT <213> Streptococcus pneumoniae mvaD <400> 128 Met Asp Arg Glu Pro Val Thr Val Arg Ser Tyr Ala Asn Ile Ala Ile 1 5 10 15 Ile Lys Tyr Trp Gly Lys Lys Lys Glu Lys Glu Met Val Pro Ala Thr 20 25 30 Ser Ser Ile Ser Leu Thr Leu Glu Asn Met Tyr Thr Glu Thr Thr Leu 35 40 45 Ser Pro Leu Pro Ala Asn Val Thr Ala Asp Glu Phe Tyr Ile Asn Gly 50 55 60 Gln Leu Gln Asn Glu Val Glu His Ala Lys Met Ser Lys Ile Ile Asp 65 70 75 80 Arg Tyr Arg Pro Ala Gly Glu Gly Phe Val Arg Ile Asp Thr Gln Asn 85 90 95 Asn Met Pro Thr Ala Ala Gly Leu Ser Ser Ser Ser Ser Gly Leu Ser 100 105 110 Ala Leu Val Lys Ala Cys Asn Ala Tyr Phe Lys Leu Gly Leu Asp Arg 115 120 125 Ser Gln Leu Ala Gln Glu Ala Lys Phe Ala Ser Gly Ser Ser Ser Arg 130 135 140 Ser Phe Tyr Gly Pro Leu Gly Ala Trp Asp Lys Asp Ser Gly Glu Ile 145 150 155 160 Tyr Pro Val Glu Thr Asp Leu Lys Leu Ala Met Ile Met Leu Val Leu 165 170 175 Glu Asp Lys Lys Lys Pro Ile Ser Ser Arg Asp Gly Met Lys Leu Cys 180 185 190 Val Glu Thr Ser Thr Thr Phe Asp Asp Trp Val Arg Gln Ser Glu Lys 195 200 205 Asp Tyr Gln Asp Met Leu Ile Tyr Leu Lys Glu Asn Asp Phe Ala Lys 210 215 220 Ile Gly Glu Leu Thr Glu Lys Asn Ala Leu Ala Met His Ala Thr Thr 225 230 235 240 Lys Thr Ala Ser Pro Ala Phe Ser Tyr Leu Thr Asp Ala Ser Tyr Glu 245 250 255 Ala Met Ala Phe Val Arg Gln Leu Arg Glu Lys Gly Glu Ala Cys Tyr 260 265 270 Phe Thr Met Asp Ala Gly Pro Asn Val Lys Val Phe Cys Gln Glu Lys 275 280 285 Asp Leu Glu His Leu Ser Glu Ile Phe Gly Gln Arg Tyr Arg Leu Ile 290 295 300 Val Ser Lys Thr Lys Asp Leu Ser Gln Asp Asp Cys Cys 305 310 315 <210> 129 <211> 182 <212> PRT <213> Escherichia coli idi <400> 129 Met Gln Thr Glu His Val Ile Leu Leu Asn Ala Gln Gly Val Pro Thr 1 5 10 15 Gly Thr Leu Glu Lys Tyr Ala Ala His Thr Ala Asp Thr Arg Leu His 20 25 30 Leu Ala Phe Ser Ser Trp Leu Phe Asn Ala Lys Gly Gln Leu Leu Val 35 40 45 Thr Arg Arg Ala Leu Ser Lys Lys Ala Trp Pro Gly Val Trp Thr Asn 50 55 60 Ser Val Cys Gly His Pro Gln Leu Gly Glu Ser Asn Glu Asp Ala Val 65 70 75 80 Ile Arg Arg Cys Arg Tyr Glu Leu Gly Val Glu Ile Thr Pro Pro Glu 85 90 95 Ser Ile Tyr Pro Asp Phe Arg Tyr Arg Ala Thr Asp Pro Ser Gly Ile 100 105 110 Val Glu Asn Glu Val Cys Pro Val Phe Ala Ala Arg Thr Thr Ser Ala 115 120 125 Leu Gln Ile Asn Asp Asp Glu Val Met Asp Tyr Gln Trp Cys Asp Leu 130 135 140 Ala Asp Val Leu His Gly Ile Asp Ala Thr Pro Trp Ala Phe Ser Pro 145 150 155 160 Trp Met Val Met Gln Ala Thr Asn Arg Glu Ala Arg Lys Arg Leu Ser 165 170 175 Ala Phe Thr Gln Leu Lys 180 <210> 130 <211> 305 <212> PRT <213> Haematococcus pluvialis ipiHp1 <400> 130 Met Leu Arg Ser Leu Leu Arg Gly Leu Thr His Ile Pro Arg Val Asn 1 5 10 15 Ser Ala Gln Gln Pro Ser Cys Ala His Ala Arg Leu Gln Phe Lys Leu 20 25 30 Arg Ser Met Gln Met Thr Leu Met Gln Pro Ser Ile Ser Ala Asn Leu 35 40 45 Ser Arg Ala Glu Asp Arg Thr Asp His Met Arg Gly Ala Ser Thr Trp 50 55 60 Ala Gly Gly Gln Ser Gln Asp Glu Leu Met Leu Lys Asp Glu Cys Ile 65 70 75 80 Leu Val Asp Val Glu Asp Asn Ile Thr Gly His Ala Ser Lys Leu Glu 85 90 95 Cys His Lys Phe Leu Pro His Gln Pro Ala Gly Leu Leu His Arg Ala 100 105 110 Phe Ser Val Phe Leu Phe Asp Asp Gln Gly Arg Leu Leu Leu Gln Gln 115 120 125 Arg Ala Arg Ser Lys Ile Thr Phe Pro Ser Val Trp Thr Asn Thr Cys 130 135 140 Cys Ser His Pro Leu His Gly Gln Thr Pro Asp Glu Val Asp Gln Leu 145 150 155 160 Ser Gln Val Ala Asp Gly Thr Val Pro Gly Ala Lys Ala Ala Ala Ile 165 170 175 Arg Lys Leu Glu His Glu Leu Gly Ile Pro Ala His Gln Leu Pro Ala 180 185 190 Ser Ala Phe Arg Phe Leu Thr Arg Leu His Tyr Cys Ala Ala Asp Val 195 200 205 Gln Pro Ala Ala Thr Gln Ser Ala Leu Trp Gly Glu His Glu Met Asp 210 215 220 Tyr Ile Leu Phe Ile Arg Ala Asn Val Thr Leu Ala Pro Asn Pro Asp 225 230 235 240 Glu Val Asp Glu Val Arg Tyr Val Thr Gln Glu Glu Leu Arg Gln Met 245 250 255 Met Gln Pro Asp Asn Gly Leu Gln Trp Ser Pro Trp Phe Arg Ile Ile 260 265 270 Ala Ala Arg Phe Leu Glu Arg Trp Trp Ala Asp Leu Asp Ala Ala Leu 275 280 285 Asn Thr Asp Lys His Glu Asp Trp Gly Thr Val His His Ile Asn Glu 290 295 300 Ala 305 <210> 131 <211> 309 <212> PRT <213> pantoea agglomerans crtB <400> 131 Met Ser Gln Pro Pro Leu Leu Asp His Ala Thr Gln Thr Met Ala Asn 1 5 10 15 Gly Ser Lys Ser Phe Ala Thr Ala Ala Lys Leu Phe Asp Pro Ala Thr 20 25 30 Arg Arg Ser Val Leu Met Leu Tyr Thr Trp Cys Arg His Cys Asp Asp 35 40 45 Val Ile Asp Asp Gln Thr His Gly Phe Ala Ser Glu Ala Ala Ala Glu 50 55 60 Glu Glu Ala Thr Gln Arg Leu Ala Arg Leu Arg Thr Leu Thr Leu Ala 65 70 75 80 Ala Phe Glu Gly Ala Glu Met Gln Asp Pro Ala Phe Ala Ala Phe Gln 85 90 95 Glu Val Ala Leu Thr His Gly Ile Thr Pro Arg Met Ala Leu Asp His 100 105 110 Leu Asp Gly Phe Ala Met Asp Val Ala Gln Thr Arg Tyr Val Thr Phe 115 120 125 Glu Asp Thr Leu Arg Tyr Cys Tyr His Val Ala Gly Val Val Gly Leu 130 135 140 Met Met Ala Arg Val Met Gly Val Arg Asp Glu Arg Val Leu Asp Arg 145 150 155 160 Ala Cys Asp Leu Gly Leu Ala Phe Gln Leu Thr Asn Ile Ala Arg Asp 165 170 175 Ile Ile Asp Asp Ala Ala Ile Asp Arg Cys Tyr Leu Pro Ala Glu Trp 180 185 190 Leu Gln Asp Ala Gly Leu Thr Pro Glu Asn Tyr Ala Ala Arg Glu Asn 195 200 205 Arg Ala Ala Leu Ala Arg Val Ala Glu Arg Leu Ile Asp Ala Ala Glu 210 215 220 Pro Tyr Tyr Ile Ser Ser Gln Ala Gly Leu His Asp Leu Pro Pro Arg 225 230 235 240 Cys Ala Trp Ala Ile Ala Thr Ala Arg Ser Val Tyr Arg Glu Ile Gly 245 250 255 Ile Lys Val Lys Ala Ala Gly Gly Ser Ala Trp Asp Arg Arg Gln His 260 265 270 Thr Ser Lys Gly Glu Lys Ile Ala Met Leu Met Ala Ala Pro Gly Gln 275 280 285 Val Ile Arg Ala Lys Thr Thr Arg Val Thr Pro Arg Pro Ala Gly Leu 290 295 300 Trp Gln Arg Pro Val 305 <210> 132 <211> 492 <212> PRT <213> pantoea agglomerans crtI <400> 132 Met Lys Lys Thr Val Val Ile Gly Ala Gly Phe Gly Gly Leu Ala Leu 1 5 10 15 Ala Ile Arg Leu Gln Ala Ala Gly Ile Pro Thr Val Leu Leu Glu Gln 20 25 30 Arg Asp Lys Pro Gly Gly Arg Ala Tyr Val Trp His Asp Gln Gly Phe 35 40 45 Thr Phe Asp Ala Gly Pro Thr Val Ile Thr Asp Pro Thr Ala Leu Glu 50 55 60 Ala Leu Phe Thr Leu Ala Gly Arg Arg Met Glu Asp Tyr Val Arg Leu 65 70 75 80 Leu Pro Val Lys Pro Phe Tyr Arg Leu Cys Trp Glu Ser Gly Lys Thr 85 90 95 Leu Asp Tyr Ala Asn Asp Ser Ala Glu Leu Glu Ala Gln Ile Thr Gln 100 105 110 Phe Asn Pro Arg Asp Val Glu Gly Tyr Arg Arg Phe Leu Ala Tyr Ser 115 120 125 Gln Ala Val Phe Gln Glu Gly Tyr Leu Arg Leu Gly Ser Val Pro Phe 130 135 140 Leu Ser Phe Arg Asp Met Leu Arg Ala Gly Pro Gln Leu Leu Lys Leu 145 150 155 160 Gln Ala Trp Gln Ser Val Tyr Gln Ser Val Ser Arg Phe Ile Glu Asp 165 170 175 Glu His Leu Arg Gln Ala Phe Ser Phe His Ser Leu Leu Val Gly Gly 180 185 190 Asn Pro Phe Thr Thr Ser Ser Ile Tyr Thr Leu Ile His Ala Leu Glu 195 200 205 Arg Glu Trp Gly Val Trp Phe Pro Glu Gly Gly Thr Gly Ala Leu Val 210 215 220 Asn Gly Met Val Lys Leu Phe Thr Asp Leu Gly Gly Glu Ile Glu Leu 225 230 235 240 Asn Ala Arg Val Glu Glu Leu Val Val Ala Asp Asn Arg Val Ser Gln 245 250 255 Val Arg Leu Ala Asp Gly Arg Ile Phe Asp Thr Asp Ala Val Ala Ser 260 265 270 Asn Ala Asp Val Val Asn Thr Tyr Lys Lys Leu Leu Gly His His Pro 275 280 285 Val Gly Gln Lys Arg Ala Ala Ala Leu Glu Arg Lys Ser Met Ser Asn 290 295 300 Ser Leu Phe Val Leu Tyr Phe Gly Leu Asn Gln Pro His Ser Gln Leu 305 310 315 320 Ala His His Thr Ile Cys Phe Gly Pro Arg Tyr Arg Glu Leu Ile Asp 325 330 335 Glu Ile Phe Thr Gly Ser Ala Leu Ala Asp Asp Phe Ser Leu Tyr Leu 340 345 350 His Ser Pro Cys Val Thr Asp Pro Ser Leu Ala Pro Pro Gly Cys Ala 355 360 365 Ser Phe Tyr Val Leu Ala Pro Val Pro His Leu Gly Asn Ala Pro Leu 370 375 380 Asp Trp Ala Gln Glu Gly Pro Lys Leu Arg Asp Arg Ile Phe Asp Tyr 385 390 395 400 Leu Glu Glu Arg Tyr Met Pro Gly Leu Arg Ser Gln Leu Val Thr Gln 405 410 415 Arg Ile Phe Thr Pro Ala Asp Phe His Asp Thr Leu Asp Ala His Leu 420 425 430 Gly Ser Ala Phe Ser Ile Glu Pro Leu Leu Thr Gln Ser Ala Trp Phe 435 440 445 Arg Pro His Asn Arg Asp Ser Asp Ile Ala Asn Leu Tyr Leu Val Gly 450 455 460 Ala Gly Thr His Pro Gly Ala Gly Ile Pro Gly Val Val Ala Ser Ala 465 470 475 480 Lys Ala Thr Ala Ser Leu Met Ile Glu Asp Leu Gln 485 490 <210> 133 <211> 382 <212> PRT <213> pantoea ananatis crtY <400> 133 Met Gln Pro His Tyr Asp Leu Ile Leu Val Gly Ala Gly Leu Ala Asn 1 5 10 15 Gly Leu Ile Ala Leu Arg Leu Gln Gln Gln Gln Pro Asp Met Arg Ile 20 25 30 Leu Leu Ile Asp Ala Ala Pro Gln Ala Gly Gly Asn His Thr Trp Ser 35 40 45 Phe His His Asp Asp Leu Thr Glu Ser Gln His Arg Trp Ile Ala Pro 50 55 60 Leu Val Val His His Trp Pro Asp Tyr Gln Val Arg Phe Pro Thr Arg 65 70 75 80 Arg Arg Lys Leu Asn Ser Gly Tyr Phe Cys Ile Thr Ser Gln Arg Phe 85 90 95 Ala Glu Val Leu Gln Arg Gln Phe Gly Pro His Leu Trp Met Asp Thr 100 105 110 Ala Val Ala Glu Val Asn Ala Glu Ser Val Arg Leu Lys Lys Gly Gln 115 120 125 Val Ile Gly Ala Arg Ala Val Ile Asp Gly Arg Gly Tyr Ala Ala Asn 130 135 140 Ser Ala Leu Ser Val Gly Phe Gln Ala Phe Ile Gly Gln Glu Trp Arg 145 150 155 160 Leu Ser His Pro His Gly Leu Ser Ser Pro Ile Ile Met Asp Ala Thr 165 170 175 Val Asp Gln Gln Asn Gly Tyr Arg Phe Val Tyr Ser Leu Pro Leu Ser 180 185 190 Pro Thr Arg Leu Leu Ile Glu Asp Thr His Tyr Ile Asp Asn Ala Thr 195 200 205 Leu Asp Pro Glu Cys Ala Arg Gln Asn Ile Cys Asp Tyr Ala Ala Gln 210 215 220 Gln Gly Trp Gln Leu Gln Thr Leu Leu Arg Glu Glu Gln Gly Ala Leu 225 230 235 240 Pro Ile Thr Leu Ser Gly Asn Ala Asp Ala Phe Trp Gln Gln Arg Pro 245 250 255 Leu Ala Cys Ser Gly Leu Arg Ala Gly Leu Phe His Pro Thr Thr Gly 260 265 270 Tyr Ser Leu Pro Leu Ala Val Ala Val Ala Asp Arg Leu Ser Ala Leu 275 280 285 Asp Val Phe Thr Ser Ala Ser Ile His His Ala Ile Thr His Phe Ala 290 295 300 Arg Glu Arg Trp Gln Gln Gln Gly Phe Phe Arg Met Leu Asn Arg Met 305 310 315 320 Leu Phe Leu Ala Gly Pro Ala Asp Ser Arg Trp Arg Val Met Gln Arg 325 330 335 Phe Tyr Gly Leu Pro Glu Asp Leu Ile Ala Arg Phe Tyr Ala Gly Lys 340 345 350 Leu Thr Leu Thr Asp Arg Leu Arg Ile Leu Ser Gly Lys Pro Pro Val 355 360 365 Pro Val Leu Ala Ala Leu Gln Ala Ile Met Thr Thr His Arg 370 375 380 <210> 134 <211> 275 <212> PRT <213> uncultured marine bacterium 66A03 SR <400> 134 Met Gly Leu Met Leu Ile Asp Trp Cys Ala Leu Ala Leu Val Val Phe 1 5 10 15 Ile Gly Leu Pro His Gly Ala Leu Asp Ala Ala Ile Ser Phe Ser Met 20 25 30 Ile Ser Ser Ala Lys Arg Ile Ala Arg Leu Ala Gly Ile Leu Leu Ile 35 40 45 Tyr Leu Leu Leu Ala Thr Ala Phe Phe Leu Ile Trp Tyr Gln Leu Pro 50 55 60 Ala Phe Ser Leu Leu Ile Phe Leu Leu Ile Ser Ile Ile His Phe Gly 65 70 75 80 Met Ala Asp Phe Asn Ala Ser Pro Ser Lys Leu Lys Trp Pro His Ile 85 90 95 Ile Ala His Gly Gly Val Val Thr Val Trp Leu Pro Leu Ile Gln Lys 100 105 110 Asn Glu Val Thr Lys Leu Phe Ser Ile Leu Thr Asn Gly Pro Thr Pro 115 120 125 Ile Leu Trp Asp Ile Leu Leu Ile Phe Phe Leu Cys Trp Ser Ile Gly 130 135 140 Val Cys Leu His Thr Tyr Glu Thr Leu Arg Ser Lys His Tyr Asn Ile 145 150 155 160 Ala Phe Glu Leu Ile Gly Leu Ile Phe Leu Ala Trp Tyr Ala Pro Pro 165 170 175 Leu Val Thr Phe Ala Thr Tyr Phe Cys Phe Ile His Ser Arg Arg His 180 185 190 Phe Ser Phe Val Trp Lys Gln Leu Gln His Met Ser Ser Lys Lys Met 195 200 205 Met Ile Gly Ser Ala Ile Ile Leu Ser Cys Thr Ser Trp Leu Ile Gly 210 215 220 Gly Gly Ile Tyr Phe Phe Leu Asn Ser Lys Met Ile Ala Ser Glu Ala 225 230 235 240 Ala Leu Gln Thr Val Phe Ile Gly Leu Ala Ala Leu Thr Val Pro His 245 250 255 Met Ile Leu Ile Asp Phe Ile Phe Arg Pro His Ser Ser Arg Ile Lys 260 265 270 Ile Lys Asn 275 <210> 135 <211> 269 <212> PRT <213> Escherichia coli MG1655 YbbO <400> 135 Met Thr His Lys Ala Thr Glu Ile Leu Thr Gly Lys Val Met Gln Lys 1 5 10 15 Ser Val Leu Ile Thr Gly Cys Ser Ser Gly Ile Gly Leu Glu Ser Ala 20 25 30 Leu Glu Leu Lys Arg Gln Gly Phe His Val Leu Ala Gly Cys Arg Lys 35 40 45 Pro Asp Asp Val Glu Arg Met Asn Ser Met Gly Phe Thr Gly Val Leu 50 55 60 Ile Asp Leu Asp Ser Pro Glu Ser Val Asp Arg Ala Ala Asp Glu Val 65 70 75 80 Ile Ala Leu Thr Asp Asn Cys Leu Tyr Gly Ile Phe Asn Asn Ala Gly 85 90 95 Phe Gly Met Tyr Gly Pro Leu Ser Thr Ile Ser Arg Ala Gln Met Glu 100 105 110 Gln Gln Phe Ser Ala Asn Phe Phe Gly Ala His Gln Leu Thr Met Arg 115 120 125 Leu Leu Pro Ala Met Leu Pro His Gly Glu Gly Arg Ile Val Met Thr 130 135 140 Ser Ser Val Met Gly Leu Ile Ser Thr Pro Gly Arg Gly Ala Tyr Ala 145 150 155 160 Ala Ser Lys Tyr Ala Leu Glu Ala Trp Ser Asp Ala Leu Arg Met Glu 165 170 175 Leu Arg His Ser Gly Ile Lys Val Ser Leu Ile Glu Pro Gly Pro Ile 180 185 190 Arg Thr Arg Phe Thr Asp Asn Val Asn Gln Thr Gln Ser Asp Lys Pro 195 200 205 Val Glu Asn Pro Gly Ile Ala Ala Arg Phe Thr Leu Gly Pro Glu Ala 210 215 220 Val Val Asp Lys Val Arg His Ala Phe Ile Ser Glu Lys Pro Lys Met 225 230 235 240 Arg Tyr Pro Val Thr Leu Val Thr Trp Ala Val Met Val Leu Lys Arg 245 250 255 Leu Leu Pro Gly Arg Val Met Asp Lys Ile Leu Gln Gly 260 265 <210> 136 <211> 620 <212> PRT <213> Escherichia coli dxs <400> 136 Met Ser Phe Asp Ile Ala Lys Tyr Pro Thr Leu Ala Leu Val Asp Ser 1 5 10 15 Thr Gln Glu Leu Arg Leu Leu Pro Lys Glu Ser Leu Pro Lys Leu Cys 20 25 30 Asp Glu Leu Arg Arg Tyr Leu Leu Asp Ser Val Ser Arg Ser Ser Gly 35 40 45 His Phe Ala Ser Gly Leu Gly Thr Val Glu Leu Thr Val Ala Leu His 50 55 60 Tyr Val Tyr Asn Thr Pro Phe Asp Gln Leu Ile Trp Asp Val Gly His 65 70 75 80 Gln Ala Tyr Pro His Lys Ile Leu Thr Gly Arg Arg Asp Lys Ile Gly 85 90 95 Thr Ile Arg Gln Lys Gly Gly Leu His Pro Phe Pro Trp Arg Gly Glu 100 105 110 Ser Glu Tyr Asp Val Leu Ser Val Gly His Ser Ser Thr Ser Ile Ser 115 120 125 Ala Gly Ile Gly Ile Ala Val Ala Ala Glu Lys Glu Gly Lys Asn Arg 130 135 140 Arg Thr Val Cys Val Ile Gly Asp Gly Ala Ile Thr Ala Gly Met Ala 145 150 155 160 Phe Glu Ala Met Asn His Ala Gly Asp Ile Arg Pro Asp Met Leu Val 165 170 175 Ile Leu Asn Asp Asn Glu Met Ser Ile Ser Glu Asn Val Gly Ala Leu 180 185 190 Asn Asn His Leu Ala Gln Leu Leu Ser Gly Lys Leu Tyr Ser Ser Leu 195 200 205 Arg Glu Gly Gly Lys Lys Val Phe Ser Gly Val Pro Pro Ile Lys Glu 210 215 220 Leu Leu Lys Arg Thr Glu Glu His Ile Lys Gly Met Val Val Pro Gly 225 230 235 240 Thr Leu Phe Glu Glu Leu Gly Phe Asn Tyr Ile Gly Pro Val Asp Gly 245 250 255 His Asp Val Leu Gly Leu Ile Thr Thr Leu Lys Asn Met Arg Asp Leu 260 265 270 Lys Gly Pro Gln Phe Leu His Ile Met Thr Lys Lys Gly Arg Gly Tyr 275 280 285 Glu Pro Ala Glu Lys Asp Pro Ile Thr Phe His Ala Val Pro Lys Phe 290 295 300 Asp Pro Ser Ser Gly Cys Leu Pro Lys Ser Ser Gly Gly Leu Pro Ser 305 310 315 320 Tyr Ser Lys Ile Phe Gly Asp Trp Leu Cys Glu Thr Ala Ala Lys Asp 325 330 335 Asn Lys Leu Met Ala Ile Thr Pro Ala Met Arg Glu Gly Ser Gly Met 340 345 350 Val Glu Phe Ser Arg Lys Phe Pro Asp Arg Tyr Phe Asp Val Ala Ile 355 360 365 Ala Glu Gln His Ala Val Thr Phe Ala Ala Gly Leu Ala Ile Gly Gly 370 375 380 Tyr Lys Pro Ile Val Ala Ile Tyr Ser Thr Phe Leu Gln Arg Ala Tyr 385 390 395 400 Asp Gln Val Leu His Asp Val Ala Ile Gln Lys Leu Pro Val Leu Phe 405 410 415 Ala Ile Asp Arg Ala Gly Ile Val Gly Ala Asp Gly Gln Thr His Gln 420 425 430 Gly Ala Phe Asp Leu Ser Tyr Leu Arg Cys Ile Pro Glu Met Val Ile 435 440 445 Met Thr Pro Ser Asp Glu Asn Glu Cys Arg Gln Met Leu Tyr Thr Gly 450 455 460 Tyr His Tyr Asn Asp Gly Pro Ser Ala Val Arg Tyr Pro Arg Gly Asn 465 470 475 480 Ala Val Gly Val Glu Leu Thr Pro Leu Glu Lys Leu Pro Ile Gly Lys 485 490 495 Gly Ile Val Lys Arg Arg Gly Glu Lys Leu Ala Ile Leu Asn Phe Gly 500 505 510 Thr Leu Met Pro Glu Ala Ala Lys Val Ala Glu Ser Leu Asn Ala Thr 515 520 525 Leu Val Asp Met Arg Phe Val Lys Pro Leu Asp Glu Ala Leu Ile Leu 530 535 540 Glu Met Ala Ala Ser His Glu Ala Leu Val Thr Val Glu Glu Asn Ala 545 550 555 560 Ile Met Gly Gly Ala Gly Ser Gly Val Asn Glu Val Leu Met Ala His 565 570 575 Arg Lys Pro Val Pro Val Leu Asn Ile Gly Leu Pro Asp Phe Phe Ile 580 585 590 Pro Gln Gly Thr Gln Glu Glu Met Arg Ala Glu Leu Gly Leu Asp Ala 595 600 605 Ala Gly Met Glu Ala Lys Ile Lys Ala Trp Leu Ala 610 615 620 <210> 137 <211> 398 <212> PRT <213> Escherichia coli dxr <400> 137 Met Lys Gln Leu Thr Ile Leu Gly Ser Thr Gly Ser Ile Gly Cys Ser 1 5 10 15 Thr Leu Asp Val Val Arg His Asn Pro Glu His Phe Arg Val Val Ala 20 25 30 Leu Val Ala Gly Lys Asn Val Thr Arg Met Val Glu Gln Cys Leu Glu 35 40 45 Phe Ser Pro Arg Tyr Ala Val Met Asp Asp Glu Ala Ser Ala Lys Leu 50 55 60 Leu Lys Thr Met Leu Gln Gln Gln Gly Ser Arg Thr Glu Val Leu Ser 65 70 75 80 Gly Gln Gln Ala Ala Cys Asp Met Ala Ala Leu Glu Asp Val Asp Gln 85 90 95 Val Met Ala Ala Ile Val Gly Ala Ala Gly Leu Leu Pro Thr Leu Ala 100 105 110 Ala Ile Arg Ala Gly Lys Thr Ile Leu Leu Ala Asn Lys Glu Ser Leu 115 120 125 Val Thr Cys Gly Arg Leu Phe Met Asp Ala Val Lys Gln Ser Lys Ala 130 135 140 Gln Leu Leu Pro Val Asp Ser Glu His Asn Ala Ile Phe Gln Ser Leu 145 150 155 160 Pro Gln Pro Ile Gln His Asn Leu Gly Tyr Ala Asp Leu Glu Gln Asn 165 170 175 Gly Val Val Ser Ile Leu Leu Thr Gly Ser Gly Gly Pro Phe Arg Glu 180 185 190 Thr Pro Leu Arg Asp Leu Ala Thr Met Thr Pro Asp Gln Ala Cys Arg 195 200 205 His Pro Asn Trp Ser Met Gly Arg Lys Ile Ser Val Asp Ser Ala Thr 210 215 220 Met Met Asn Lys Gly Leu Glu Tyr Ile Glu Ala Arg Trp Leu Phe Asn 225 230 235 240 Ala Ser Ala Ser Gln Met Glu Val Leu Ile His Pro Gln Ser Val Ile 245 250 255 His Ser Met Val Arg Tyr Gln Asp Gly Ser Val Leu Ala Gln Leu Gly 260 265 270 Glu Pro Asp Met Arg Thr Pro Ile Ala His Thr Met Ala Trp Pro Asn 275 280 285 Arg Val Asn Ser Gly Val Lys Pro Leu Asp Phe Cys Lys Leu Ser Ala 290 295 300 Leu Thr Phe Ala Ala Pro Asp Tyr Asp Arg Tyr Pro Cys Leu Lys Leu 305 310 315 320 Ala Met Glu Ala Phe Glu Gln Gly Gln Ala Ala Thr Thr Ala Leu Asn 325 330 335 Ala Ala Asn Glu Ile Thr Val Ala Ala Phe Leu Ala Gln Gln Ile Arg 340 345 350 Phe Thr Asp Ile Ala Ala Leu Asn Leu Ser Val Leu Glu Lys Met Asp 355 360 365 Met Arg Glu Pro Gln Cys Val Asp Asp Val Leu Ser Val Asp Ala Asn 370 375 380 Ala Arg Glu Val Ala Arg Lys Glu Val Met Arg Leu Ala Ser 385 390 395 <210> 138 <211> 299 <212> PRT <213> Escherichia coli ispA <400> 138 Met Asp Phe Pro Gln Gln Leu Glu Ala Cys Val Lys Gln Ala Asn Gln 1 5 10 15 Ala Leu Ser Arg Phe Ile Ala Pro Leu Pro Phe Gln Asn Thr Pro Val 20 25 30 Val Glu Thr Met Gln Tyr Gly Ala Leu Leu Gly Gly Lys Arg Leu Arg 35 40 45 Pro Phe Leu Val Tyr Ala Thr Gly His Met Phe Gly Val Ser Thr Asn 50 55 60 Thr Leu Asp Ala Pro Ala Ala Ala Val Glu Cys Ile His Ala Tyr Ser 65 70 75 80 Leu Ile His Asp Asp Leu Pro Ala Met Asp Asp Asp Asp Leu Arg Arg 85 90 95 Gly Leu Pro Thr Cys His Val Lys Phe Gly Glu Ala Asn Ala Ile Leu 100 105 110 Ala Gly Asp Ala Leu Gln Thr Leu Ala Phe Ser Ile Leu Ser Asp Ala 115 120 125 Asp Met Pro Glu Val Ser Asp Arg Asp Arg Ile Ser Met Ile Ser Glu 130 135 140 Leu Ala Ser Ala Ser Gly Ile Ala Gly Met Cys Gly Gly Gln Ala Leu 145 150 155 160 Asp Leu Asp Ala Glu Gly Lys His Val Pro Leu Asp Ala Leu Glu Arg 165 170 175 Ile His Arg His Lys Thr Gly Ala Leu Ile Arg Ala Ala Val Arg Leu 180 185 190 Gly Ala Leu Ser Ala Gly Asp Lys Gly Arg Arg Ala Leu Pro Val Leu 195 200 205 Asp Lys Tyr Ala Glu Ser Ile Gly Leu Ala Phe Gln Val Gln Asp Asp 210 215 220 Ile Leu Asp Val Val Gly Asp Thr Ala Thr Leu Gly Lys Arg Gln Gly 225 230 235 240 Ala Asp Gln Gln Leu Gly Lys Ser Thr Tyr Pro Ala Leu Leu Gly Leu 245 250 255 Glu Gln Ala Arg Lys Lys Ala Arg Asp Leu Ile Asp Asp Ala Arg Gln 260 265 270 Ser Leu Lys Gln Leu Ala Glu Gln Ser Leu Asp Thr Ser Ala Leu Glu 275 280 285 Ala Leu Ala Asp Tyr Ile Ile Gln Arg Asn Lys 290 295 <210> 139 <211> 551 <212> PRT <213> Clausena lansium STS <400> 139 Met Ser Thr Gln Gln Val Ser Ser Glu Asn Ile Val Arg Asn Ala Ala 1 5 10 15 Asn Phe His Pro Asn Ile Trp Gly Asn His Phe Leu Thr Cys Pro Ser 20 25 30 Gln Thr Ile Asp Ser Trp Thr Gln Gln His His Lys Glu Leu Lys Glu 35 40 45 Glu Val Arg Lys Met Met Val Ser Asp Ala Asn Lys Pro Ala Gln Arg 50 55 60 Leu Arg Leu Ile Asp Thr Val Gln Arg Leu Gly Val Ala Tyr His Phe 65 70 75 80 Glu Lys Glu Ile Asp Asp Ala Leu Glu Lys Ile Gly His Asp Pro Phe 85 90 95 Asp Asp Lys Asp Asp Leu Tyr Ile Val Ser Leu Cys Phe Arg Leu Leu 100 105 110 Arg Gln His Gly Ile Lys Ile Ser Cys Asp Val Phe Glu Lys Phe Lys 115 120 125 Asp Asp Asp Gly Lys Phe Lys Ala Ser Leu Met Asn Asp Val Gln Gly 130 135 140 Met Leu Ser Leu Tyr Glu Ala Ala His Leu Ala Ile His Gly Glu Asp 145 150 155 160 Ile Leu Asp Glu Ala Ile Val Phe Thr Thr Thr His Leu Lys Ser Thr 165 170 175 Val Ser Asn Ser Pro Val Asn Ser Thr Phe Ala Glu Gln Ile Arg His 180 185 190 Ser Leu Arg Val Pro Leu Arg Lys Ala Val Pro Arg Leu Glu Ser Arg 195 200 205 Tyr Phe Leu Asp Ile Tyr Ser Arg Asp Asp Leu His Asp Lys Thr Leu 210 215 220 Leu Asn Phe Ala Lys Leu Asp Phe Asn Ile Leu Gln Ala Met His Gln 225 230 235 240 Lys Glu Ala Ser Glu Met Thr Arg Trp Trp Arg Asp Phe Asp Phe Leu 245 250 255 Lys Lys Leu Pro Tyr Ile Arg Asp Arg Val Val Glu Leu Tyr Phe Trp 260 265 270 Ile Leu Val Gly Val Ser Tyr Gln Pro Lys Phe Ser Thr Gly Arg Ile 275 280 285 Phe Leu Ser Lys Ile Ile Cys Leu Glu Thr Leu Val Asp Asp Thr Phe 290 295 300 Asp Ala Tyr Gly Thr Phe Asp Glu Leu Ala Ile Phe Thr Glu Ala Val 305 310 315 320 Thr Arg Trp Asp Leu Gly His Arg Asp Ala Leu Pro Glu Tyr Met Lys 325 330 335 Phe Ile Phe Lys Thr Leu Ile Asp Val Tyr Ser Glu Ala Glu Gln Glu 340 345 350 Leu Ala Lys Glu Gly Arg Ser Tyr Ser Ile His Tyr Ala Ile Arg Ser 355 360 365 Phe Gln Glu Leu Val Met Lys Tyr Phe Cys Glu Ala Lys Trp Leu Asn 370 375 380 Lys Gly Tyr Val Pro Ser Leu Asp Asp Tyr Lys Ser Val Ser Leu Arg 385 390 395 400 Ser Ile Gly Phe Leu Pro Ile Ala Val Ala Ser Phe Val Phe Met Gly 405 410 415 Asp Ile Ala Thr Lys Glu Val Phe Glu Trp Glu Met Asn Asn Pro Lys 420 425 430 Ile Ile Ile Ala Ala Glu Thr Ile Phe Arg Phe Leu Asp Asp Ile Ala 435 440 445 Gly His Arg Phe Glu Gln Lys Arg Glu His Ser Pro Ser Ala Ile Glu 450 455 460 Cys Tyr Lys Asn Gln His Gly Val Ser Glu Glu Glu Ala Val Lys Ala 465 470 475 480 Leu Ser Leu Glu Val Ala Asn Ser Trp Lys Asp Ile Asn Glu Glu Leu 485 490 495 Leu Leu Asn Pro Met Ala Ile Pro Leu Pro Leu Leu Gln Val Ile Leu 500 505 510 Asp Leu Ser Arg Ser Ala Asp Phe Met Tyr Gly Asn Ala Gln Asp Arg 515 520 525 Phe Thr His Ser Thr Met Met Lys Asp Gln Val Asp Leu Val Leu Lys 530 535 540 Asp Pro Val Lys Leu Asp Asp 545 550 <210> 140 <211> 572 <212> PRT <213> Artificial Sequence <220> <223> Matricaria recutita MrBBS <400> 140 Met Ser Thr Leu Ser Val Ser Thr Pro Ser Phe Ser Ser Ser Pro Leu 1 5 10 15 Ser Ser Val Asn Lys Asn Ser Thr Lys Gln His Val Thr Arg Asn Ser 20 25 30 Val Ile Phe His Asp Ser Ile Trp Gly Asp Gln Phe Leu Glu Tyr Lys 35 40 45 Glu Lys Phe Asn Val Ala Thr Glu Lys Gln Leu Ile Glu Glu Leu Lys 50 55 60 Glu Glu Val Arg Asn Glu Leu Met Ile Arg Ala Cys Asn Glu Ala Ser 65 70 75 80 Arg Tyr Ile Lys Leu Ile Gln Leu Ile Asp Val Val Glu Arg Leu Gly 85 90 95 Leu Ala Tyr His Phe Glu Lys Glu Ile Glu Glu Ser Leu Gln His Ile 100 105 110 Tyr Val Thr Tyr Gly His Lys Trp Thr Asn Tyr Asn Asn Ile Glu Ser 115 120 125 Leu Ser Leu Trp Phe Arg Leu Leu Arg Gln Asn Gly Phe Asn Val Ser 130 135 140 Ser Asp Ile Phe Glu Asn His Ile Asp Glu Lys Gly Asn Phe Gln Glu 145 150 155 160 Ser Leu Cys Asn Asp Pro Gln Gly Met Leu Ala Leu Tyr Glu Ala Ala 165 170 175 Tyr Met Arg Val Glu Gly Glu Ile Ile Leu Asp Lys Ala Leu Glu Phe 180 185 190 Thr Lys Leu His Leu Gly Ile Ile Ser Asn Asp Pro Ser Cys Asp Ser 195 200 205 Ser Leu Arg Thr Glu Ile Lys Gln Ala Leu Lys Gln Pro Leu Arg Arg 210 215 220 Arg Leu Pro Arg Leu Glu Ala Val Arg Tyr Ile Ala Ile Tyr Gln Gln 225 230 235 240 Lys Ala Ser His Ser Glu Val Leu Leu Lys Leu Ala Lys Leu Asp Phe 245 250 255 Asn Val Leu Gln Glu Met His Lys Asp Glu Leu Ser Gln Ile Cys Lys 260 265 270 Trp Trp Lys Asp Leu Asp Ile Arg Asn Lys Leu Pro Tyr Val Arg Asp 275 280 285 Arg Leu Ile Glu Gly Tyr Phe Trp Ile Leu Gly Ile Tyr Phe Glu Pro 290 295 300 Gln His Ser Arg Thr Arg Met Phe Leu Met Lys Thr Cys Met Trp Leu 305 310 315 320 Ile Val Leu Asp Asp Thr Phe Asp Asn Tyr Gly Thr Tyr Glu Glu Leu 325 330 335 Glu Ile Phe Thr Gln Ala Val Glu Arg Trp Ser Ile Thr Cys Leu Asp 340 345 350 Glu Leu Pro Glu Tyr Met Lys Leu Ile Tyr His Glu Gln Phe Arg Val 355 360 365 His Gln Glu Met Glu Glu Ser Leu Glu Lys Glu Gly Lys Ala Tyr Gln 370 375 380 Ile His Tyr Ile Lys Glu Met Ala Lys Glu Gly Thr Arg Ser Leu Leu 385 390 395 400 Leu Glu Ala Lys Trp Leu Lys Glu Gly Tyr Met Pro Thr Leu Asp Glu 405 410 415 Tyr Leu Ser Asn Ser Leu Val Thr Cys Gly Tyr Ala Leu Met Thr Ala 420 425 430 Arg Ser Tyr Val Ala Arg Asp Asp Gly Ile Val Thr Glu Asp Ala Phe 435 440 445 Lys Trp Val Ala Thr His Pro Pro Ile Val Lys Ala Ala Cys Lys Ile 450 455 460 Leu Arg Leu Met Asp Asp Ile Ala Thr His Lys Glu Glu Gln Glu Arg 465 470 475 480 Gly His Ile Ala Ser Ser Ile Glu Cys Tyr Arg Lys Glu Thr Gly Ala 485 490 495 Ser Glu Glu Glu Ala Cys Met Asp Phe Leu Lys Gln Val Glu Asp Gly 500 505 510 Trp Lys Val Ile Asn Gln Glu Ser Leu Met Pro Thr Asp Val Pro Phe 515 520 525 Pro Leu Leu Ile Pro Ala Ile Asn Leu Ala Arg Val Ser Asp Thr Leu 530 535 540 Tyr Lys Asp Asn Asp Gly Tyr Asn His Ala Asp Lys Glu Val Ile Gly 545 550 555 560 Tyr Ile Lys Ser Leu Phe Val His Pro Met Ile Val 565 570 <210> 141 <211> 391 <212> PRT <213> Artificial Sequence <220> <223> EGFP <400> 141 Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 1 5 10 15 Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 20 25 30 Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 35 40 45 Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 50 55 60 Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 65 70 75 80 Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 85 90 95 Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 100 105 110 Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 115 120 125 Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 130 135 140 Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 145 150 155 160 Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 165 170 175 Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 180 185 190 Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 195 200 205 Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 210 215 220 Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Gly 225 230 235 240 Tyr Pro Ala Gly Trp Arg Pro Leu Glu Met Val Glu Arg Lys Lys Leu 245 250 255 Gly Val Leu Gln Pro Thr Gln Gln Cys Arg Leu Thr Val His Gln Ser 260 265 270 Lys Ile Met Val Asp Ser Pro Lys Ala Leu Lys Arg Lys Ala Phe Gly 275 280 285 Asp Glu Asn Phe Ser Pro Val Pro Thr Leu Ser Arg Lys Ser Ile Phe 290 295 300 Glu Asp Pro Pro Thr Pro Ala Ser Lys Arg Ser Lys Leu Ser Asp Lys 305 310 315 320 Val Asp Ser Ala Ile Gln Thr Asp Asn Leu Cys Cys Gly Asn Gly Gly 325 330 335 Thr Glu Ile Ser Arg Gly Ser Ser Ser Ser Lys Ser Ser Ser Glu Ala 340 345 350 Ser Thr Lys Ala Cys Ser Gln Lys Ser Gln Ser Gln Ser Val Leu Asp 355 360 365 Met Leu Thr Ser Ala Glu Pro Asn Glu Gln Tyr Trp Gln Met Met Ala 370 375 380 Ala Glu Arg Lys Ala Ala Leu 385 390 <210> 142 <211> 410 <212> PRT <213> Lactococcus lactis subsp. cremoris NZ9000 mvaA <400> 142 Met Arg Lys Lys Phe Tyr Gln Met Ser Pro Gln Glu Arg Leu Asn Ser 1 5 10 15 Leu Asn Leu Ser Glu Lys Ser Gln Glu Ile Leu Ser Glu Met Ala Leu 20 25 30 Asp Thr Thr Ile Leu Asp Asn Leu Ile Glu Asn Gln Ile Ser Glu Phe 35 40 45 Glu Leu Pro Met Gly Ile Ala Gln Asn Phe Val Ile Asn Gly Gln Ser 50 55 60 Phe Leu Ile Pro Met Val Thr Glu Glu Pro Ser Val Ile Ala Ala Ala 65 70 75 80 Ser Asn Gly Ala Lys Ile Ala Gly Asn Phe Val Ala Glu Ile Lys Glu 85 90 95 Arg Leu Met Arg Gly Gln Ile Val Phe Tyr Asp Val Lys Asn Ser Asp 100 105 110 Lys Ile Ala Asn Glu Ile Leu Glu Lys Gln Glu Lys Ile Phe Glu Gln 115 120 125 Ala Glu Leu Ser Tyr Pro Ser Ile Val Lys Arg Gly Gly Gly Leu Arg 130 135 140 Glu Val Ser Ser Arg Ile Phe Ser Ser Gln Lys Phe Leu Ser Val Asp 145 150 155 160 Val Lys Val Asp Val Lys Asp Ala Met Gly Ala Asn Ile Ile Asn Ser 165 170 175 Ile Leu Glu Gly Ile Ala Glu Leu Phe Arg Arg Trp Phe Pro Asp Glu 180 185 190 Lys Ile Leu Phe Ser Ile Leu Ser Asn Tyr Ala Thr Glu Ser Leu Val 195 200 205 Lys Val Thr Cys Glu Ile Pro Val Glu Arg Leu Ser Lys Lys Ala Asp 210 215 220 Gly Tyr Glu Ile Gly Gln Lys Ile Met Ala Ala Ser Gln Tyr Ser Lys 225 230 235 240 Ile Asp Pro Tyr Arg Ala Ser Thr His Asn Lys Gly Ile Met Asn Gly 245 250 255 Ile Asn Ala Val Ile Leu Ala Thr Gly Asn Asp Thr Arg Ala Ile Ser 260 265 270 Ala Ala Ile His Ala Tyr Ala Ala Lys Asp Gly Ala Tyr Gln Gly Leu 275 280 285 Ala Asn Trp Glu Leu Gln Glu Lys Met Leu Val Gly Glu Leu Glu Phe 290 295 300 Pro Leu Pro Val Ala Thr Val Gly Gly Gly Val Lys Val Leu Pro Lys 305 310 315 320 Ala Gln Ala Ala Met Glu Ile Leu Gly Ile Ser Asp Ala Lys Glu Leu 325 330 335 Ala Lys Val Ile Ala Ala Val Gly Leu Ala Gln Asn Leu Ala Ala Leu 340 345 350 Arg Ala Leu Val Ser Glu Gly Ile Gln Gln Gly His Met Ser Leu Gln 355 360 365 Ala Arg Ser Leu Ala Leu Ser Val Gly Ala Gln Ala Asp Glu Ile Ala 370 375 380 Ile Leu Ser Gln Gln Leu Arg Lys Glu Lys Val Met Asn Gln Glu Val 385 390 395 400 Ala Gln Asn Leu Leu Lys Asn Leu Arg Lys 405 410 <210> 143 <211> 410 <212> PRT <213> Artificial Sequence <220> <223> Lactococcus lactis subsp. cremoris MG1363 Hydroxymethylglutaryl-CoA reductase <400> 143 Met Arg Lys Lys Phe Tyr Gln Met Ser Pro Gln Glu Arg Leu Asn Ser 1 5 10 15 Leu Asn Leu Ser Glu Lys Ser Gln Glu Ile Leu Ser Glu Met Ala Leu 20 25 30 Asp Thr Thr Ile Leu Asp Asn Leu Ile Glu Asn Gln Ile Ser Glu Phe 35 40 45 Glu Leu Pro Met Gly Ile Ala Gln Asn Phe Val Ile Asn Gly Gln Ser 50 55 60 Phe Leu Ile Pro Met Val Thr Glu Glu Pro Ser Val Ile Ala Ala Ala 65 70 75 80 Ser Asn Gly Ala Lys Ile Ala Gly Asn Phe Val Ala Glu Ile Lys Glu 85 90 95 Arg Leu Met Arg Gly Gln Ile Val Phe Tyr Asp Val Lys Asn Ser Asp 100 105 110 Lys Ile Ala Asn Glu Ile Leu Glu Lys Gln Glu Lys Ile Phe Glu Gln 115 120 125 Ala Glu Leu Ser Tyr Pro Ser Ile Val Lys Arg Gly Gly Gly Leu Arg 130 135 140 Glu Val Ser Ser Arg Ile Phe Ser Ser Gln Lys Phe Leu Ser Val Asp 145 150 155 160 Val Lys Val Asp Val Lys Asp Ala Met Gly Ala Asn Ile Ile Asn Ser 165 170 175 Ile Leu Glu Gly Ile Ala Glu Leu Phe Arg Arg Trp Phe Pro Asp Glu 180 185 190 Lys Ile Leu Phe Ser Ile Leu Ser Asn Tyr Ala Thr Glu Ser Leu Val 195 200 205 Lys Val Thr Cys Glu Ile Pro Val Glu Arg Leu Ser Lys Lys Ala Asp 210 215 220 Gly Tyr Glu Ile Gly Gln Lys Ile Met Ala Ala Ser Gln Tyr Ser Lys 225 230 235 240 Ile Asp Pro Tyr Arg Ala Ser Thr His Asn Lys Gly Ile Met Asn Gly 245 250 255 Ile Asn Ala Val Ile Leu Ala Thr Gly Asn Asp Thr Arg Ala Ile Ser 260 265 270 Ala Ala Ile His Ala Tyr Ala Ala Lys Asp Gly Ala Tyr Gln Gly Leu 275 280 285 Ala Asn Trp Glu Leu Gln Glu Lys Met Leu Val Gly Glu Leu Glu Phe 290 295 300 Pro Leu Pro Val Ala Thr Val Gly Gly Gly Val Lys Val Leu Pro Lys 305 310 315 320 Ala Gln Ala Ala Met Glu Ile Leu Gly Ile Ser Asp Ala Lys Glu Leu 325 330 335 Ala Lys Val Ile Ala Ala Val Gly Leu Ala Gln Asn Leu Ala Ala Leu 340 345 350 Arg Ala Leu Val Ser Glu Gly Ile Gln Gln Gly His Met Ser Leu Gln 355 360 365 Ala Arg Ser Leu Ala Leu Ser Val Gly Ala Gln Ala Asp Glu Ile Ala 370 375 380 Ile Leu Ser Gln Gln Leu Arg Lys Glu Lys Val Met Asn Gln Glu Val 385 390 395 400 Ala Gln Asn Leu Leu Lys Asn Leu Arg Lys 405 410

Claims (34)

  1. 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로의 효소를 코딩하는 유전자 중 적어도 하나가 도입된 플라스미드를 포함하는 형질전환 생물체 선별용 마커 조성물.
  2. 청구항 1에 있어서, 상기 생물체는 내재적으로 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로를 갖는 것인 형질전환 생물체 선별용 마커 조성물.
  3. 청구항 1에 있어서, 상기 합성 경로는 MEP 경로 또는 MVA 경로인 형질전환 생물체 선별용 마커 조성물.
  4. 청구항 1에 있어서, 상기 합성 경로의 효소를 코딩하는 유전자는 1-디옥시-D-자이룰로스-5-포스페이트(DXP) 신타아제, DXP 리덕토이소머라아제, 2-C-메틸-D-에리스리톨-4-포스페이트(MEP) 시티딜트랜스퍼라아제, 4-디포스포시티딜-2-C-메틸-D-에리스리톨 키나아제, 2-C-메틸-D-에리스리톨-2,4-시클로디포스페이트(MEcPP) 신타아제, 4-히드록시-3-메틸-2-부테닐 디포스페이트 (HMBPP) 신타아제, HMBPP 리덕타아제, 아세토아세틸-CoA 신타아제, 3-히드록실-3-메틸글루타리-CoA (HMG-CoA) 신타아제, HMG-CoA 리덕타아제, 메발로네이트 키나아제, 메발로네이트-5-포스페이트 키나아제, 메발로네이트-5-디포스페이트 디카르복실라아제 및 IPP 아이소머라아제로 이루어진 군에서 선택된 1종 이상의 효소를 코딩하는 유전자인 형질전환 생물체 선별용 마커 조성물.
  5. 청구항 1에 있어서, 상기 조성물은 상기와 동일 효소를 코딩하는 유전자 또는 이의 보완 유전자가 감쇄 또는 결손된 생물체에 형질전환 되는 것인 형질전환 생물체 선별용 마커 조성물.
  6. 청구항 5에 있어서, 상기 유전자는 MEP 경로의 효소를 코딩하는 유전자이고, 상기 보완 유전자는 MVA 경로의 효소 중 적어도 하나를 코딩하는 유전자인 형질전환 생물체 선별용 마커 조성물.
  7. 청구항 6에 있어서, 상기 보완 유전자는 아세토아세틸-CoA 신타아제, 3-히드록실-3-메틸글루타리-CoA (HMG-CoA) 신타아제, HMG-CoA 리덕타아제, 메발로네이트 키나아제, 메발로네이트-5-포스페이트 키나아제, 메발로네이트-5-디포스페이트 디카르복실라아제 및 IPP 아이소머라아제를 코딩하는 유전자인 형질전환 생물체 선별용 마커 조성물.
  8. 청구항 5에 있어서, 상기 유전자는 MVA 경로의 효소를 코딩하는 유전자이고, 상기 보완 유전자는 MEP 경로의 효소 중 적어도 하나를 코딩하는 유전자인 형질전환 생물체 선별용 마커 조성물.
  9. 청구항 1에 있어서, 상기 유전자 중 적어도 2개를 포함하고, 이들은 별개의 플라스미드에 각각 도입된 것인 형질전환 생물체 선별용 마커 조성물.
  10. 청구항 1에 있어서, 상기 플라스미드는 이소프레노이드, 산탈렌, 비사볼롤 및 레티놀 합성 경로 이루어진 군에서 선택된 경로의 효소를 코딩하는 유전자가 더 도입된 것인 형질전환 생물체 선별용 마커 조성물.
  11. 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로의 효소를 코딩하는 유전자 중 적어도 하나가 감쇄 또는 결손되고, 상기 감쇄 또는 결손된 유전자와 동일 효소를 코딩하는 유전자 또는 그 보완 유전자가 도입된 플라스미드로 형질전환된 생물체.
  12. 청구항 11에 있어서, 상기 생물체는 내재적으로 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로를 갖는 것인 생물체.
  13. 청구항 11에 있어서, 상기 합성 경로는 MEP 경로 또는 MVA 경로인 생물체.
  14. 청구항 11에 있어서, 상기 합성 경로의 효소를 코딩하는 유전자는 1-디옥시-D-자이룰로스-5-포스페이트(DXP) 신타아제, DXP 리덕토이소머라아제, 2-C-메틸-D-에리스리톨-4-포스페이트(MEP) 시티딜트랜스퍼라아제, 4-디포스포시티딜-2-C-메틸-D-에리스리톨 키나아제, 2-C-메틸-D-에리스리톨-2,4-시클로디포스페이트(MEcPP) 신타아제, 4-히드록시-3-메틸-2-부테닐 디포스페이트 (HMBPP) 신타아제, HMBPP 리덕타아제, 아세토아세틸-CoA 신타아제, 3-히드록실-3-메틸글루타리-CoA (HMG-CoA) 신타아제, HMG-CoA 리덕타아제, 메발로네이트 키나아제, 메발로네이트-5-포스페이트 키나아제, 메발로네이트-5-디포스페이트 디카르복실라아제 및 IPP 아이소머라아제로 이루어진 군에서 선택된 1종 이상의 효소를 코딩하는 유전자인 생물체.
  15. 청구항 11에 있어서, 상기 감쇄 또는 결손되는 유전자는 MEP 경로 상의 효소를 코딩하는 유전자인 생물체.
  16. 청구항 15에 있어서, 상기 유전자는 DXP 신타아제 및 DXP 리덕토이소머라아제 중 적어도 하나를 코딩하는 유전자인 생물체.
  17. 청구항 11에 있어서, 상기 감쇄 또는 결손되는 유전자는 MEP 경로 상의 효소를 코딩하는 유전자이고, 상기 보완 유전자는 MVA 경로 상의 효소 중 적어도 하나를 코딩하는 유전자인 생물체.
  18. 청구항 17에 있어서, 상기 보완 유전자는 아세토아세틸-CoA 신타아제, 3-히드록실-3-메틸글루타리-CoA (HMG-CoA) 신타아제, HMG-CoA 리덕타아제, 메발로네이트 키나아제, 메발로네이트-5-포스페이트 키나아제, 메발로네이트-5-디포스페이트 디카르복실라아제 및 IPP 아이소머라아제를 코딩하는 유전자인 생물체.
  19. 청구항 11에 있어서, 상기 감쇄 또는 결손되는 유전자는 MVA 경로 상의 효소를 코딩하는 유전자인 생물체.
  20. 청구항 11에 있어서, 상기 플라스미드는 이소프레노이드, 산탈렌, 비사볼롤 및 레티놀 합성 경로 이루어진 군에서 선택된 경로의 효소를 코딩하는 유전자가 더 도입된 것인 생물체.
  21. 형질전환 대상 생물체의 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로의 효소를 코딩하는 유전자 중 적어도 하나를 감쇄 또는 결손시키는 단계; 및
    상기 생물체를 감쇄 또는 결손된 유전자와 동일 효소를 코딩하는 유전자 또는 이의 보완 유전자가 도입된 재조합 플라스미드로 형질전환시키는 단계;를 포함하는 생물체의 형질 전환 방법.
  22. 청구항 21에 있어서, 상기 생물체는 내재적으로 이소펜테닐 디포스페이트 또는 디메틸알릴 디포스페이트 합성 경로를 갖는 것인 생물체의 형질 전환 방법.
  23. 청구항 21에 있어서, 상기 형질 전환은 항생제 없이 수행되는 것인 생물체의 형질 전환 방법.
  24. 청구항 21에 있어서, 상기 합성 경로는 MEP 경로 또는 MVA 경로인 생물체의 형질 전환 방법.
  25. 청구항 21에 있어서, 상기 합성 경로의 효소를 코딩하는 유전자는 1-디옥시-D-자이룰로스-5-포스페이트(DXP) 신타아제, DXP 리덕토이소머라아제, 2-C-메틸-D-에리스리톨-4-포스페이트(MEP) 시티딜트랜스퍼라아제, 4-디포스포시티딜-2-C-메틸-D-에리스리톨 키나아제(IspE), 2-C-메틸-D-에리스리톨-2,4-시클로디포스페이트(MEcPP) 신타아제, 4-히드록시-3-메틸-2-부테닐 디포스페이트 (HMBPP) 신타아제, HMBPP 리덕타아제, 아세토아세틸-CoA 신타아제, 3-히드록실-3-메틸글루타리-CoA (HMG-CoA) 신타아제, HMG-CoA 리덕타아제, 메발로네이트 키나아제, 메발로네이트-5-포스페이트 키나아제, 메발로네이트-5-디포스페이트 디카르복실라아제 및 IPP 아이소머라아제 로 이루어진 군에서 선택된 1종 이상의 효소를 코딩하는 유전자인 생물체의 형질 전환 방법.
  26. 청구항 21에 있어서, 상기 감쇄 또는 결손되는 유전자는 MEP 경로 상의 효소를 코딩하는 유전자인 생물체의 형질 전환 방법.
  27. 청구항 26에 있어서, 상기 유전자는 DXP 신타아제 및 DXP 리덕토이소머라아제 중 적어도 하나를 코딩하는 유전자인 생물체의 형질 전환 방법.
  28. 청구항 21에 있어서, 상기 감쇄 또는 결손되는 유전자는 MEP 경로 상의 효소를 코딩하는 유전자이고, 상기 보완 유전자는 MVA 경로 상의 효소 중 적어도 하나를 코딩하는 유전자인 생물체의 형질 전환 방법.
  29. 청구항 28에 있어서, 상기 보완 유전자는 아세토아세틸-CoA 신타아제, 3-히드록실-3-메틸글루타리-CoA (HMG-CoA) 신타아제, HMG-CoA 리덕타아제, 메발로네이트 키나아제, 메발로네이트-5-포스페이트 키나아제, 메발로네이트-5-디포스페이트 디카르복실라아제 및 IPP 아이소머라아제를 코딩하는 유전자인 생물체의 형질 전환 방법.
  30. 청구항 21에 있어서, 상기 감쇄 또는 결손되는 유전자는 MVA 경로 상의 효소 중 적어도 하나를 코딩하는 유전자인 생물체의 형질 전환 방법.
  31. 청구항 21에 있어서, 상기 플라스미드는 이소프레노이드, 산탈렌, 비사볼롤 및 레티놀 합성 경로 이루어진 군에서 선택된 경로의 효소를 코딩하는 유전자를 더 포함하는 생물체의 형질 전환 방법.
  32. 청구항 21에 있어서, 적어도 2개의 유전자를 감쇄 또는 결손시키고, 상기 균주를 이와 동일한 효소를 코딩하는 유전자를 각각 포함하는 적어도 2개의 플라스미드로 형질전환시키는 생물체의 형질 전환 방법.
  33. 청구항 11 내지 20 중 어느 한 항의 생물체를 기질이 포함된 배지 상에서 배양하는 단계를 포함하는 목적 산물의 생산 방법.
  34. 청구항 33에 있어서, 상기 배지는 항생제를 포함하지 않는 것인 목적 산물의 생산 방법.
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