CN101631798A - 产生非节段负链rna病毒的细胞和方法 - Google Patents

产生非节段负链rna病毒的细胞和方法 Download PDF

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CN101631798A
CN101631798A CN200780047473A CN200780047473A CN101631798A CN 101631798 A CN101631798 A CN 101631798A CN 200780047473 A CN200780047473 A CN 200780047473A CN 200780047473 A CN200780047473 A CN 200780047473A CN 101631798 A CN101631798 A CN 101631798A
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cell
ser
ile
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F·坦吉
P·沙尔诺
Y·雅各布
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Centre National de la Recherche Scientifique CNRS
Institut Pasteur
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CN200780047473A 2006-12-22 2007-12-21 产生非节段负链rna病毒的细胞和方法 Pending CN101631798A (zh)

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EP06292025.1A EP1939214B1 (en) 2006-12-22 2006-12-22 Cells and methodology to generate non-segmented negative-strand RNA viruses
EP06292025.1 2006-12-22

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103906843A (zh) * 2011-06-08 2014-07-02 维什瓦斯·乔希 双质粒哺乳动物表达系统
CN109355301A (zh) * 2018-08-22 2019-02-19 深圳大学 一种石斑鱼重组NNVcp的密码子优化的核酸序列、融合表达载体、制备方法及其本身
WO2019061691A1 (zh) * 2017-09-26 2019-04-04 王红伟 一种可诱导性拯救弹状病毒的细胞系及其构建方法与应用

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1375512T3 (da) 2002-06-20 2009-10-26 Pasteur Institut Infektiös cDNA af en godkendt vaccinestamme af mæslingevirus. Anvendelse til immunogene sammensætninger
DK1375670T3 (da) 2002-06-20 2013-09-23 Pasteur Institut Rekombinante mæslingevira, der udtrykker epitoper af RNA-viras antigener, samt anvendelse af de rekombinante vira til fremstilling af vaccinesammensætninger
CA2432738A1 (en) 2003-02-26 2004-08-26 Philippe Despres New dengue and west nile viruses proteins and genes coding the foregoing, and their use in vaccinal, therapeutic and diagnostic applications
WO2012038832A2 (en) * 2010-09-24 2012-03-29 Institut Pasteur Generation of replicating chimeric measles virus - retrovirus particles
FR2994697B1 (fr) * 2012-08-24 2016-05-13 Centre De Coop Int En Rech Agronomique Pour Le Dev (Cirad) Souche vaccinale marquee du virus de la peste des petits ruminants, et son procede d'obtention
EP2712871A1 (en) 2012-09-27 2014-04-02 Institut Pasteur Recombinant Measles virus expressing Chikungunya virus polypeptides and their applications
EP2759301A1 (en) 2013-01-24 2014-07-30 Institut Pasteur Use of a genetically modified infectious measles virus with enhanced pro-apoptotic properties (MV-DeltaC virus)
US20140255447A1 (en) * 2013-03-05 2014-09-11 Biomune Company Production of avian embryo cells
WO2015092710A1 (en) 2013-12-19 2015-06-25 Glaxosmithkline Biologicals, S.A. Contralateral co-administration of vaccines
GB2583296A (en) 2015-01-05 2020-10-21 Seagull Biosolutions Pvt Ltd Dna molecules producing custom designed replicating and non-replicating negative stranded rna viruses and uses there of
EP3184119A1 (en) 2015-12-23 2017-06-28 Themis Bioscience GmbH Chromatography based purification strategies for measles scaffold based viruses
EP3255056A1 (en) * 2016-06-06 2017-12-13 Commissariat À L'Énergie Atomique Et Aux Énergies Alternatives Method for producing self-assemblying paramyxoviral nucleocapsid-like particles and their uses
EP3622078A1 (en) 2017-05-09 2020-03-18 Invectys Recombinant measles vaccine expressing htert
EP3412307A1 (en) 2017-06-07 2018-12-12 Institut Pasteur Recombinant measles virus expressing zika virus proteins and their applications
BR112020009157A2 (pt) 2017-11-09 2020-10-27 Institut Pasteur poliepítopo quimérico, polinucleotídeo, vetor, célula hospedeira e composição imunogênica
EP3727411A2 (en) 2017-12-21 2020-10-28 Institut Pasteur Lassa vaccine
WO2020012037A1 (en) * 2018-07-13 2020-01-16 Valneva Se Method for rescuing and producing a virus in avian cells
CN111218474A (zh) * 2018-11-23 2020-06-02 成都生物制品研究所有限责任公司 一种副粘病毒的拯救系统和副粘病毒拯救方法
EP3673917A1 (en) 2018-12-28 2020-07-01 Themis Bioscience GmbH Norovirus vaccines
CN109913423A (zh) * 2019-03-27 2019-06-21 扬州大学 一种稳定表达猪德尔塔冠状病毒N蛋白的重组Vero细胞系及应用
EP3865180A1 (en) 2020-02-13 2021-08-18 Institut Pasteur Live recombinant measles virus expressing coronavirus antigens - its use in eliciting immunity against coronaviruses
MX2022009989A (es) 2020-02-13 2022-11-08 Pasteur Institut Composiciones inmunogenicas y vacunas para covid-19 vectorizadas por sarampion.
US20230310581A1 (en) * 2020-04-27 2023-10-05 Ohio State Innovation Foundation A live attenuated measles virus vectored vaccine for sars-cov-2
EP3936517A1 (en) * 2020-07-08 2022-01-12 Institut Pasteur An improved measles virus vaccine vector based on multiple tandem additional transcription units
AU2022208199A1 (en) 2021-01-13 2023-07-13 Centre National De La Recherche Scientifique Measles-hiv or measles-htlv vaccine
WO2023230598A2 (en) * 2022-05-27 2023-11-30 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Reverse genetics system for feline morbillivirus
EP4501946A1 (en) 2023-07-31 2025-02-05 LoopLab Bio GmbH Self-amplifying biologics for targeted therapy
WO2026013289A1 (en) 2024-07-12 2026-01-15 Institut Pasteur Measles-mumps-rubella (mmr) vaccines
CN120648655B (zh) * 2025-08-21 2026-02-13 内蒙古自治区农牧业科学院 一株t7-293t细胞及其在牛副流感病毒3型感染性克隆拯救病毒中的应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997006270A1 (en) * 1995-08-09 1997-02-20 Schweiz. Serum- & Impfinstitut Bern cDNA CORRESPONDING TO THE ANTIGENOME OF NONSEGMENTED NEGATIVE STRAND RNA VIRUSES, AND PROCESS FOR THE PRODUCTION OF SUCH VIRUSES ENCODING ADDITIONAL ANTIGENICALLY ACTIVE PROTEINS

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60033045T2 (de) 1999-10-12 2007-11-08 Institut Pasteur Lentivirale triplex-dns, und vektoren und rekombinante zellen, die lentivirale triplex-dns enthalten
JP2004503233A (ja) 2000-06-16 2004-02-05 インスティティ・パスツール デング熱ウイルスエンベロープ糖タンパク質のプロ−アポトーシスフラグメント
DK1375670T3 (da) 2002-06-20 2013-09-23 Pasteur Institut Rekombinante mæslingevira, der udtrykker epitoper af RNA-viras antigener, samt anvendelse af de rekombinante vira til fremstilling af vaccinesammensætninger
DE10234579A1 (de) * 2002-07-30 2004-02-12 Zf Friedrichshafen Ag Getriebe
KR101020532B1 (ko) * 2003-06-09 2011-03-09 와이어쓰 엘엘씨 cDNA로부터의 비분절 네가티브 쇄 RNA 바이러스의 회수를 위한 개선된 방법
EP2361974A1 (fr) 2003-12-02 2011-08-31 Institut Pasteur Utilisation des proteines et des peptides codés par le genome d'une nouvelle souche de coronavirus associé au SRAS
FR2870126B1 (fr) 2004-05-17 2009-07-17 Pasteur Institut Vecteur lentiviral recombinant d'expression d'une proteine de flaviviridae et ses applications comme vaccin
US20080248067A1 (en) * 2005-04-14 2008-10-09 The University Of Queensland Immunomodulating Compositions and Uses Therefor

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997006270A1 (en) * 1995-08-09 1997-02-20 Schweiz. Serum- & Impfinstitut Bern cDNA CORRESPONDING TO THE ANTIGENOME OF NONSEGMENTED NEGATIVE STRAND RNA VIRUSES, AND PROCESS FOR THE PRODUCTION OF SUCH VIRUSES ENCODING ADDITIONAL ANTIGENICALLY ACTIVE PROTEINS

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
AUDE SIRCEN等: "Enhanced Transgene Expression in Cord Blood CD34+-Derived Hematopoietic Cells, Including Developing T Cells and NOD/SCID Mouse Repopulating Cells,Following Transduction with Modified TRIP Lentiviral Vectors", 《MOLECULAR THERAPY》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103906843A (zh) * 2011-06-08 2014-07-02 维什瓦斯·乔希 双质粒哺乳动物表达系统
CN103906843B (zh) * 2011-06-08 2016-12-07 维什瓦斯·乔希 双质粒哺乳动物表达系统
WO2019061691A1 (zh) * 2017-09-26 2019-04-04 王红伟 一种可诱导性拯救弹状病毒的细胞系及其构建方法与应用
CN109355301A (zh) * 2018-08-22 2019-02-19 深圳大学 一种石斑鱼重组NNVcp的密码子优化的核酸序列、融合表达载体、制备方法及其本身

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EP1939214A1 (en) 2008-07-02
CN104745533A (zh) 2015-07-01
US20100144040A1 (en) 2010-06-10
JP2010512779A (ja) 2010-04-30
WO2008078198A3 (en) 2008-10-30
BRPI0719469A2 (pt) 2014-02-11
ES2423518T3 (es) 2013-09-20
US9499799B2 (en) 2016-11-22
EP2091962B1 (en) 2018-07-04
ES2686168T3 (es) 2018-10-16
DK1939214T3 (da) 2013-10-14
US8586364B2 (en) 2013-11-19
US20140051148A1 (en) 2014-02-20
KR20090103922A (ko) 2009-10-01
BRPI0719469B1 (pt) 2019-07-02
KR101469007B1 (ko) 2014-12-05
MX2009006755A (es) 2009-07-31
EP1939214B1 (en) 2013-07-10
DK2091962T3 (en) 2018-10-08
JP2015015955A (ja) 2015-01-29
JP5902371B2 (ja) 2016-04-13
WO2008078198A2 (en) 2008-07-03
BRPI0719469C1 (pt) 2021-05-25
CA2673262C (en) 2019-03-05
CA2673262A1 (en) 2008-07-03
EP2091962A2 (en) 2009-08-26

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