CN101611663A - A kind of tobacco seed germination accelerating method - Google Patents
A kind of tobacco seed germination accelerating method Download PDFInfo
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- CN101611663A CN101611663A CN200910157338A CN200910157338A CN101611663A CN 101611663 A CN101611663 A CN 101611663A CN 200910157338 A CN200910157338 A CN 200910157338A CN 200910157338 A CN200910157338 A CN 200910157338A CN 101611663 A CN101611663 A CN 101611663A
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Abstract
The invention discloses a kind of tobacco seed germination accelerating method, this method utilizes Gibberellins solution to break seed dormancy, promotes tobacco seed to germinate by steps such as seed soaking, cleaning, sterilization, vernalization, the regularity that improves bud ratio and sprout, reach that seedling is neat, seedling is strong, improves the purpose of the quality of growing seedlings.
Description
Technical field
The present invention relates to a kind of tobacco seed germination accelerating method, relate in particular to a kind of tobacco seed germination accelerating method that utilizes the gibberellin breaking dormancy.
Background technology
Fan Zhi tobacco new seed is owing to exist resting stage then, and germination need be broken seed dormancy, generally breaks seed dormancy and needs light induction, can also adopt stranding kind technology to break seed dormancy.In the tradition germination accelerating method, after soaking seed under 25～28 ℃ of conditions, rub with the hands and plant, enter the vernalization state afterwards, promote to emerge.But a large amount of tobacco seed vernalization generally are placed in the cloth bag and carry out in the leaf tobacco production, are difficult to satisfy illumination condition, artificial germination is difficulty relatively, and influence is grown seedlings, and rubs the difficult grasp of the technology of planting with the hands, rub kind of the excessive easy stranding of dynamics with the hands and gone bad embryo, the dynamics of stranding is little not to reach effect, and seed does not germinate.Therefore it is undesirable to break seed dormant method effect in traditional tobacco seed germination accelerating method, makes germination rate lower.
Summary of the invention
Technical problem to be solved by this invention provides a kind of tobacco seed germination accelerating method, this method utilizes certain density Gibberellins solution to handle tobacco seed can breaking dormancy, stratification, the regularity that improves bud ratio and sprout, reach that seedling is neat, seedling is strong, improves the purpose of the quality of growing seedlings.
For solving the problems of the technologies described above, technical scheme provided by the present invention is:
A kind of tobacco seed germination accelerating method provided by the invention may further comprise the steps:
(1) seed soaking: tobacco seed directly poured into fill in the vessel that concentration is 90～110mg/kg Gibberellins solution, seed is immersed in the Gibberellins solution fully, temperature keeps 20～28 ℃, seed soaking;
(2) clean: after finishing seed soaking, remove solution, clean seed 2～3 times with clear water, the cloth bag of then seed being packed into, tying dries with drier;
(3) sterilization: place 1% copper-bath to sterilize 10～15 minutes seed packet, control out the cloth bag herb liquid,, all dry after washing at every turn with drier with 25～28 ℃ of flushing with clean water seed packets 2～3 times;
(4) vernalization: after sterilization, the drying, seed packet is ridden on the support of culturing room or be hung in the culturing room, temperature remains on 28～30 ℃, preceding 3 days of vernalization, every day is with 25～28 ℃ of flushing with clean water 1 time, each flushing back dries with drier, opens every 2～3 hours that bag stirs seed or the moving seed of string 1 time in bag; Vernalization after the 3rd day seed begin to grin, dry with drier with 25～28 ℃ of flushing with clean water 2～3 times, each flushing back every day, opens every 1～2 hour that bag stirs seed or the moving seed of string 1 time in bag.
In the described step (1), seed soaking time is 72～78 hours.
The compound method of described Gibberellins solution is: get gibberellin, and earlier with the alcohol dissolving, and then 90～110mg/kg Gibberellins solution that is mixed with soluble in water.
In the described step (1), when soaking seed, stir 1～2 time in per 24 hours, seed is fully immersed in the liquid, increase the oxygen in the liquid simultaneously, stratification with Gibberellins solution.
In the described step (4), also be included in the seed packet outside and cover wet cloth or spray low amounts of water,, make temperature be controlled at 28～30 ℃ to keep the humidity of seed packet appearance with sprayer.
Because tobacco seed is when rudiment, factors such as moisture, temperature, oxygen are very important to the influence of sprouting.Therefore, open at set intervals in the said method that bag stirs seed or in bag the moving seed of string be to be the gas exchange that guarantees in the seed packet, also help tobacco seed and sprout comparatively neat; Each with the refuse of flushing with clean water in can the flush away metabolic process.The presprouting of seeds later stage will be noted frequent observation, can increase flushing and open that bag stirs seed or in bag the moving seed number of times of string, in time cooling prevents to burn bud.
The advantage that the present invention has:
1. traditional germination accelerating method break Seed Dormancy by illumination, stranding kind of a technology, but the most applications illumination condition can not satisfy, and stranding kind of dynamics is not enough, and seed does not germinate, or germinates seldom, and bud ratio only has 5%～25%.The present invention adopts gibberellin to soak seed, replaces the illumination breaking dormancy, does not need to rub kind of a sport technique segment with the hands, and germination rate reaches 90%～95%, and effect is remarkable, has solved the difficult problem in the leaf tobacco production.
2. behind the each water flushing of the present invention seed, all adopt the drier dehydration.Because tobacco seed kind grain is little, the surface forms moisture film easily, and influence is ventilative, causes not germinateing.Utilize drier dehydration can remove the moisture film on tobacco seed surface, both maintained certain moisture, the purpose that can ventilate again, stratification.
Embodiment
Adopt embodiment that the present invention is described in further detail below.
Embodiment 1
The tobacco seed germination accelerating method may further comprise the steps:
(1) seed soaking: 1 kilogram of tobacco seed directly poured into fill in the vessel that concentration is the 90mg/kg Gibberellins solution, the amount of Gibberellins solution will guarantee to make seed to immerse fully wherein, and temperature keeps 20～28 ℃, soaks seed 72 hours;
(2) clean: after finishing seed soaking, remove solution, clean seed 2 times with clear water, the cloth bag of then seed being packed into, tying dries with drier;
(3) sterilization: seed packet was placed 1% copper-bath sterilization 10 minutes, control out the cloth bag herb liquid,, all dry after washing at every turn with drier with 25～28 ℃ of flushing with clean water seed packets 2 times;
(4) vernalization: after sterilization, the drying, seed packet is ridden on the rod of culturing room or be hung in the culturing room, temperature remains on 28～30 ℃, preceding 3 days of vernalization, every day is with 25～28 ℃ of flushing with clean water 1 time, each flushing back dries with drier, opens every 2 hours that bag stirs seed or the moving seed of string 1 time in bag; Vernalization after the 3rd day seed begin to grin, dry with drier with 25～28 ℃ of flushing with clean water 2 times, each flushing back every day, opens every 1 hour that bag stirs seed or the moving seed of string 1 time in bag.
The compound method of described Gibberellins solution is: get 0.9 gram gibberellin, dissolve with 20 ml ethanols, and then be dissolved in and be mixed with the 90mg/kg Gibberellins solution in the 10kg water.
In the described step (1), when soaking seed, stir 1 time in per 24 hours, seed is fully immersed in the liquid, increase the oxygen in the liquid simultaneously, stratification with Gibberellins solution.
In the described step (4), also be included in the seed packet outside and cover wet cloth or spray low amounts of water,, make temperature be controlled at 28～30 ℃ to keep the humidity of seed packet appearance with sprayer.
The germination rate of this method can reach 90%.
Embodiment 2
The tobacco seed germination accelerating method may further comprise the steps:
(1) seed soaking: 1 kilogram of tobacco seed directly poured into fill in the vessel that concentration is the 100mg/kg Gibberellins solution, the amount of Gibberellins solution will guarantee to make seed to immerse fully wherein, and temperature keeps 20～28 ℃, soaks seed 78 hours;
(2) clean: after finishing seed soaking, remove solution, clean seed 3 times with clear water, the cloth bag of then seed being packed into, tying dries with drier;
(3) sterilization: seed packet was placed 1% copper-bath sterilization 15 minutes, control out the cloth bag herb liquid,, all dry after washing at every turn with drier with 25～28 ℃ of flushing with clean water seed packets 3 times;
(4) vernalization: after sterilization, the drying, seed packet is ridden on the rod of culturing room or be hung in the culturing room, temperature remains on 28～30 ℃, preceding 3 days of vernalization, every day is with 25～28 ℃ of flushing with clean water 1 time, each flushing back dries with drier, opens every 3 hours that bag stirs seed or the moving seed of string 1 time in bag; Vernalization after the 3rd day seed begin to grin, dry with drier with 25～28 ℃ of flushing with clean water 3 times, each flushing back every day, opens every 2 hours that bag stirs seed or the moving seed of string 1 time in bag.
The compound method of described Gibberellins solution is: get 1 gram gibberellin, dissolve with 20 ml ethanols, and then be dissolved in and be mixed with the 100mg/kg Gibberellins solution in the 10kg water.
In the described step (1), when soaking seed, stir 2 times in per 24 hours, seed is fully immersed in the liquid, increase the oxygen in the liquid simultaneously, stratification with Gibberellins solution.
In the described step (4), also be included in the seed packet outside and cover wet cloth or spray low amounts of water,, make temperature be controlled at 28～30 ℃ to keep the humidity of seed packet appearance with sprayer.
The germination rate of this method can reach 95%.
Embodiment 3
The tobacco seed germination accelerating method may further comprise the steps:
(1) seed soaking: 1 kilogram of tobacco seed directly poured into fill in the vessel that concentration is the 110mg/kg Gibberellins solution, the amount of Gibberellins solution will guarantee to make seed to immerse fully wherein, and temperature keeps 20～28 ℃, soaks seed 72 hours;
(2) clean: after finishing seed soaking, remove solution, clean seed 2 times with clear water, the cloth bag of then seed being packed into, tying dries with drier;
(3) sterilization: seed packet was placed 1% copper-bath sterilization 10 minutes, control out the cloth bag herb liquid,, all dry after washing at every turn with drier with 25～28 ℃ of flushing with clean water seed packets 2 times;
(4) vernalization: after sterilization, the drying, seed packet is ridden on the rod of culturing room or be hung in the culturing room, temperature remains on 28～30 ℃, preceding 3 days of vernalization, every day is with 25～28 ℃ of flushing with clean water 1 time, each flushing back dries with drier, opens every 2 hours that bag stirs seed or the moving seed of string 1 time in bag; Vernalization after the 3rd day seed begin to grin, dry with drier with 25～28 ℃ of flushing with clean water 2 times, each flushing back every day, opens every 1 hour that bag stirs seed or the moving seed of string 1 time in bag.
The compound method of described Gibberellins solution is: get 1.1 gram gibberellin, dissolve with 20 ml ethanols, and then be dissolved in and be mixed with the 110mg/kg Gibberellins solution in the 10kg water.
In the described step (1), when soaking seed, stir 1 time in per 24 hours, seed is fully immersed in the liquid, increase the oxygen in the liquid simultaneously, stratification with Gibberellins solution.
In the described step (4), also be included in the seed packet outside and cover wet cloth or spray low amounts of water,, make temperature be controlled at 28～30 ℃ to keep the humidity of seed packet appearance with sprayer.
The germination rate of this method can reach 92%.
Claims (5)
1, a kind of tobacco seed germination accelerating method is characterized in that, may further comprise the steps:
(1) seed soaking: tobacco seed directly poured into fill in the vessel that concentration is 90～110mg/kg Gibberellins solution, seed is immersed in the Gibberellins solution fully, temperature keeps 20～28 ℃, seed soaking;
(2) clean: after finishing seed soaking, remove solution, clean seed 2～3 times with clear water, the cloth bag of then seed being packed into, tying dries with drier;
(3) sterilization: place 1% copper-bath to sterilize 10～15 minutes seed packet, control out the cloth bag herb liquid,, all dry after washing at every turn with drier with 25～28 ℃ of flushing with clean water seed packets 2～3 times;
(4) vernalization: after sterilization dries seed packet is ridden on the support of culturing room or be hung in the culturing room, temperature remains on 28～30 ℃, preceding 3 days of vernalization, every day is with 25～28 ℃ of flushing with clean water 1 time, each flushing back dries with drier, opens every 2～3 hours that bag stirs seed or the moving seed of string 1 time in bag; Vernalization after the 3rd day seed begin to grin, dry with drier with 25～28 ℃ of flushing with clean water 2～3 times, each flushing back every day, opens every 1～2 hour that bag stirs seed or the moving seed of string 1 time in bag.
2, tobacco seed germination accelerating method according to claim 1 is characterized in that, in the described step (1), seed soaking time is 72～78 hours.
3, tobacco seed germination accelerating method according to claim 1 is characterized in that, the compound method of described Gibberellins solution is: get gibberellin, and earlier with the alcohol dissolving, and then concentration 90～110mg/kg Gibberellins solution that is mixed with soluble in water.
4, according to the described tobacco seed germination accelerating method of claim 1, it is characterized in that, in the described step (1), when soaking seed, stir 1～2 time in per 24 hours with Gibberellins solution.
5, according to the described tobacco seed germination accelerating method of claim 1, it is characterized in that, in the described step (4), also be included in the seed packet outside and cover wet cloth or spray low amounts of water with sprayer.
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| CN200910157338A CN101611663A (en) | 2009-07-24 | 2009-07-24 | A kind of tobacco seed germination accelerating method |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102415236A (en) * | 2011-08-19 | 2012-04-18 | 青岛中烟种子有限责任公司 | Complete nutrition formula germination accelerating method for tobacco seed |
| CN102523786A (en) * | 2011-12-27 | 2012-07-04 | 云南省烟草农业科学研究院 | Preparation method for novel tobacco seed germination bed |
| CN102661954A (en) * | 2012-04-12 | 2012-09-12 | 楚天科技股份有限公司 | Automatic lamp inspector |
| CN102870578A (en) * | 2012-10-23 | 2013-01-16 | 段然 | Sprout production process |
| CN103340045A (en) * | 2013-07-15 | 2013-10-09 | 贵州省烟草科学研究院 | Method for water separation and sterilization of tobacco seeds |
| CN104054477A (en) * | 2014-05-15 | 2014-09-24 | 和县华禾种业有限公司 | Method for accelerating germination of cucurbita pepo seeds |
| CN104067802A (en) * | 2014-06-19 | 2014-10-01 | 安徽百卉园林有限公司 | Method for increasing germination rate of nandina seeds |
| CN104885616A (en) * | 2014-09-29 | 2015-09-09 | 安徽盛创农业科技有限公司 | Tobacco seed germination method |
| CN106304888A (en) * | 2016-08-17 | 2017-01-11 | 桂林市蔬菜研究所 | Method for accelerating germination of rootstocks |
| CN110959334A (en) * | 2019-12-14 | 2020-04-07 | 玉溪中烟种子有限责任公司 | Germination culture medium for improving activity of tobacco seeds under low-temperature condition |
-
2009
- 2009-07-24 CN CN200910157338A patent/CN101611663A/en active Pending
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102415236A (en) * | 2011-08-19 | 2012-04-18 | 青岛中烟种子有限责任公司 | Complete nutrition formula germination accelerating method for tobacco seed |
| CN102523786A (en) * | 2011-12-27 | 2012-07-04 | 云南省烟草农业科学研究院 | Preparation method for novel tobacco seed germination bed |
| CN102661954A (en) * | 2012-04-12 | 2012-09-12 | 楚天科技股份有限公司 | Automatic lamp inspector |
| CN102870578A (en) * | 2012-10-23 | 2013-01-16 | 段然 | Sprout production process |
| CN103340045A (en) * | 2013-07-15 | 2013-10-09 | 贵州省烟草科学研究院 | Method for water separation and sterilization of tobacco seeds |
| CN104054477B (en) * | 2014-05-15 | 2016-02-17 | 和县华禾种业有限公司 | A kind of custard squash seed accelerating germination method |
| CN104054477A (en) * | 2014-05-15 | 2014-09-24 | 和县华禾种业有限公司 | Method for accelerating germination of cucurbita pepo seeds |
| CN104067802A (en) * | 2014-06-19 | 2014-10-01 | 安徽百卉园林有限公司 | Method for increasing germination rate of nandina seeds |
| CN104067802B (en) * | 2014-06-19 | 2016-01-06 | 安徽百卉园林有限公司 | A kind of method improving heavenly bamboo seed germination rate |
| CN104885616A (en) * | 2014-09-29 | 2015-09-09 | 安徽盛创农业科技有限公司 | Tobacco seed germination method |
| CN106304888A (en) * | 2016-08-17 | 2017-01-11 | 桂林市蔬菜研究所 | Method for accelerating germination of rootstocks |
| CN106304888B (en) * | 2016-08-17 | 2019-04-19 | 桂林市蔬菜研究所 | A kind of germination accelerating method of stock |
| CN110959334A (en) * | 2019-12-14 | 2020-04-07 | 玉溪中烟种子有限责任公司 | Germination culture medium for improving activity of tobacco seeds under low-temperature condition |
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