CN102415236A - Complete nutrition formula germination accelerating method for tobacco seed - Google Patents
Complete nutrition formula germination accelerating method for tobacco seed Download PDFInfo
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- CN102415236A CN102415236A CN2011102389330A CN201110238933A CN102415236A CN 102415236 A CN102415236 A CN 102415236A CN 2011102389330 A CN2011102389330 A CN 2011102389330A CN 201110238933 A CN201110238933 A CN 201110238933A CN 102415236 A CN102415236 A CN 102415236A
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Abstract
The invention belongs to the technical field of germination accelerating treatment and specifically relates to a complete nutrition formula germination accelerating method for tobacco seeds. The method comprises the following steps: putting tobacco seeds in a nutrient solution of MS+100 mg / L gibberellin + 2 mg / L naphthylacetic acid and accelerating germination for 42-46 h under conditions of a temperature of 26-28 DEG C, 12 h illumination / 12 h dark and continuous air feeding for oxygen supplementation; taking the seeds out of a germination accelerating pot, when most of the seeds have broken seed coats, and drying; carrying out coating and pelleting according to a routine technology to obtain the required germination accelerating-type pelleted tobacco seed. The germination accelerating-type pelleted tobacco seed prepared by the invention has advantages of high seed activity, fast sprouting, high rate of emergence and high uniformity, etc.
Description
Technical field
The invention belongs to the presprouting of seeds processing technology field, be specifically related to a kind of tobacco seed complete nutritional formula germination accelerating method.
Background technology
Tobacco seed is the important foundation of leaf tobacco production.Research shows that preliminary treatment can obviously improve the vigor of seed before broadcasting, and makes and after planting emerges neatly, and seedling is healthy and strong, and vernalization is wherein a kind of simple and effective preceding preprocess method of broadcasting, and can improve the emergence rate and the uniformity of seed.Mainly utilizing water and Gibberellins solution that tobacco seed is carried out vernalization on producing at present handles.And in seed germination and growth of seedling process, except that the suitable illumination of needs, temperature, moisture, plant growth regulator such as methyl and macronutrient, micronutrient element all play an important role.But still rarely report at present, about the correlative study that utilizes nutritive element to combine to carry out tobacco vernalization to handle with plant growth regulator.In view of this, system research of the present invention variable concentrations MS culture fluid, GA
3, NAA influence that tobacco seed vernalization is handled; Confirmed significantly effectively to improve the complete nutritional formula nutrient solution of tobacco seed vigor, germination rate and the regularity of emerging; And in tobacco agriculture is produced, verified and the remarkable result of this invention scientific basis is provided for further improving tobacco vernalization capsuled seed quality.
Summary of the invention
The objective of the invention is weak point to prior art; A kind of tobacco seed complete nutritional formula germination accelerating method is provided; With solve tobacco agriculture in producing ubiquitous emerge slow, emergence rate is low and emerge problem such as irregular; Behind the inventive method processing tobacco seed, can carry and shorten tobacco seed sprout time, raising emergence rate and seedling regularity, raising cigarette seedling quality.
The objective of the invention is to realize through following technical scheme.
The invention provides a kind of tobacco seed complete nutritional formula germination accelerating method, may further comprise the steps:
Tobacco seed is put into the nutritive solution of MS+100mg/L gibberellin+2mg/L methyl, in temperature be 26-28 ℃, 12h illumination/12h is dark and the condition of lasting bubbling air oxygenating under vernalization 42h-46h.
Before presprouting of seeds, can put into the weight ratio 0.5% copper-bath 15min that sterilizes, clean with distilled water flushing again, dry, be used for vernalization.
Germination temperature is preferably 27 ℃, and germination time is preferably 46h.
During vernalization, connect air compressor, seed is suspended in the nutrient solution.
Tobacco seed after vernalization is handled can be processed the tobacco pelleted seed after nucleation, Pelleting, typing, polishing and the dyeing of routine, pack is broadcast fully.
Compared with prior art, the present invention has following beneficial effect:
Seed is lived organism, and the vigor of tobacco seed is that growth, maturation, the storage along with seed constantly changes, and promptly when physiological ripening, it is the highest that seed vitality reaches, and decline in various degree takes place seed vitality subsequently.Behind seed collection, holding conditions with broadcast before preliminary treatment be even more important.Research shows that preliminary treatment can obviously improve the vigor of seed before broadcasting, and makes and after planting emerges neatly, and seedling is healthy and strong.The vernalization technology that the present invention adopts is a kind of simple and effective method.Tobacco seed is through MS nutrient solution+100mg/L gibberellin (GA
3After the vernalization of)+2mg/L methyl (NAA) complete nutrition solution was handled, the vigor of seed significantly improved, and sprout time is short, and emergence rate is high and emerge neat; Seedling development is fast, and cigarette seedling quality improves.For solving long, the problem irregular, that cigarette seedling quality is poor, cigarette strain growth retardation, quality descend of emerging of the seed germination time that tobacco exists in producing, explored a new effective way.
Description of drawings
Fig. 1 is that the influence curve figure to the tobacco seed germination rate is handled in the vernalization of variable concentrations gibberellin;
Fig. 2 is that the influence curve figure to the tobacco seed germination rate is handled in the vernalization of variable concentrations methyl;
Fig. 3 is that the influence curve figure to the tobacco seed germination rate is handled in the vernalization of MS nutrient solution;
Fig. 4 is that second day germination rate sketch map of back sowing is handled in different vernalization;
1: without vernalization; 2:H
2O+50mg/L (unit down together) NAA; 3:H
2O+2NAA; 4:H
2O+5NAA; 5:H
2O+10NAA; 6:H
2O+20NAA; 7:H
2O+50NAA; 8:H
2O+100GA; 9:H
2O+100GA+2NAA; 10:H
2O+100GA+5NAA; 11:H
2O+100GA+10NAA; 12:H
2O+100GA+20NAA; 13:H
2O+100GA+50NAA; 14:H
2O+300GA; 15:H
2O+300GA+2NAA; 16:H
2O+300GA+5NAA; 17:H
2O+300GA+10NAA; 18:H
2O+300GA+20NAA; 19:H
2O+300GA+50NAA; 20:H
2O+500GA; 21:H
2O+500GA+2NAA; 22:H
2O+500GA+5NAA; 23:H
2O+500GA+10NAA; 24:H
2O+500GA+20NAA; 25:H
2O+500GA+50NAA; 26:MS; 27:MS+2NAA; 28:MS+5NAA; 29:MS+10NAA; 30:MS+20NAA; 31:MS+50NAA; 32:MS+100GA; 33:MS+100GA+2NAA; 34:MS+100GA+5NAA; 35:MS+100GA+10NAA; 36:MS+100GA+20NAA; 37:MS+100GA+50NAA; 38:MS+300GA; 39:MS+300GA+2NAA; 40:MS+300GA+5NAA; 41:MS+300GA+10NAA; 42:MS+300GA+20NAA; 43:MS+300GA+50NAA; 44:MS+500GA; 45:MS+500GA+2NAA; 46:MS+500GA+5NAA; 47:MS+500GA+10NAA; 48:MS+500GA+20NAA; 49:MS+500GA+50NAA
Fig. 5 uses up the influence sketch map of pancebrin vernalization to the tobacco seed sprouting;
Fig. 6 uses up the influence curve figure that pancebrin vernalization is sprouted tobacco seed;
Fig. 7 is the influence sketch map of complete nutrition liquid vernalization to the tobacco seed growth of seedling; Bar:5mm
Fig. 8 is the influence sketch map of complete nutrition liquid vernalization to tobacco seed seedling root length;
Fig. 9 is the influence sketch map of complete nutrition liquid vernalization to tobacco seed seedling hypocotyl length.
Embodiment
Through specific embodiment given below, can further be well understood to the present invention.But they are not to qualification of the present invention.
Embodiment
(1) experimental technique
(1) supply the examination tobacco seed: experimental cultivar is middle cigarette 201 one-level breedings, is provided by cigarette seed Co., Ltd in the Qingdao.
(2) the culture fluid setting of vernalization processing: gibberellin (GA
3) establish 0,100mg/L, 300mg/L, four concentration gradients of 500mg/L; Methyl (NAA) establishes 0,2mg/L, 5mg/L, 10mg/L, 20mg/L, six concentration gradients of 50mg/L; Basic culture solution is H
2O, MS liquid nutrient medium (Murashige and Skoog, 1962), the combination of three factor completely randoms, 48 processing, three repetitions.
(3) vernalization: with weight ratio 0.5% copper-bath sterilization 15min, with distilled water flushing for several times, 30 ℃ are dried to water content about 10%, carry out vernalization with the above-mentioned culture fluid for preparing then seed more earlier.Vernalization is carried out in the vernalization jar, connects air compressor, and seed can be suspended in the nutrient solution that is full of air; Illumination condition is that 12h illumination/12h is dark, and temperature is 26-28 ℃ (preferred 27 ℃), and germination time is 42-46h (preferred 46h).After vernalization finished, 30 ℃ were dried to water content about 10%, preserved with subsequent use.If do not handle with a water vernalization and handle two contrasts through vernalization.
(4) germination test: evenly sowing is in culture dish for seed, and bottom is the moistening absorbent cotton pad of one deck in the culture dish, its upper berth one deck filter paper.Place culture dish in the artificial lighting incubator, temperature is 27 ± 1 ℃, and 12h illumination/12h is dark.Each processing is provided with three repetitions, 100 seeds of each repeat sowing.
(5) data statistics: from after planting first day to the 7th day, add up seed germination quantity every day, draw to sprout curve.After planting the 7th day statistics germination vigor, after planting fortnight statistics germination rate.
Subnumber * 100% is planted experimentally in normal germinative number/confession in the germination vigor (%)=7 day
Subnumber * 100% is planted experimentally in normal germinative number/confession in the germination rate (%)=14 day
(6) seed germination state recording: began to the 4th day from after planting first day; Select each to handle five seeds of the inside at random; Thereafter every day fixing to these seeds with the preservation of taking pictures of OLYMPUS SZX2-ILLK type light microscope, write down the seed germination state of respectively handling.After planting fortnight is handled seedling with each and is scanned the preservation image, simultaneously long the and hypocotyl length with its root of photoshop software measurement.
(2) experimental result
1, gibberellin (GA
3) vernalization handles the influence to the tobacco seed germination rate
Gibberellin (GA3) is one type of important plant growth regulating hormone, in plant growth and development process, plays crucial effect, can remove seed dormancy, improves seed vitality, therefore in agricultural production, has obtained extensive use.Can know by Fig. 1 sprout time curve; At after planting second day; The seed of not handling through vernalization is not also sprouted; It is 22% that water carries out the germination rate that vernalization handles, with 100mg/L, 300mg/L and 500mg/L gibberellin vernalization processing be respectively 42%, 48% and 62%, explain that the germination rate that is lower than tobacco seed under the situation of 500mg/L in gibberellin concentration improves with the increase of gibberellin treatment concentration.
Can be found out also that by Fig. 1 after gibberellin vernalization was handled, middle cigarette 201 seed germinating times had had obviously in advance, and germination vigor compares also according to wanting high with germination rate, showing that gibberellin vernalization is handled can stimulate tobacco seed to sprout, shortening cigarette seedling seedling-growing time.
2, the influence to the tobacco seed germination rate is handled in methyl (NAA) vernalization
Methyl (NAA) is a kind of plant growth regulator of broad spectrum activity, many processes of involved in plant g and D, as the formation of the aging of the growth of root and stem, organ, vascular bundle tissue and differentiation and development and plant to ground and phototropic reaction etc.Visible by Fig. 2, after handling with 2mg/L and the vernalization of 5mg/L methyl, germination rate was 50% and 45% in after planting second day, was significantly higher than the seed of handling (0) and independent water vernalization processing (22%) without vernalization.But along with the continuation of concentration increases, this facilitation weakens gradually.This shows that carrying out the vernalization processing with NAA separately can obviously improve the tobacco seed germination rate, and 2mg/L～5mg/L is an optimum concentration; But when high concentration, facilitation weakens gradually, even can suppress seed germination.
3, the influence to the tobacco seed germination rate is handled in the vernalization of MS liquid nutrient medium
The normal growth of tobacco needs an amount of various nutritive elements with maturation, and nutrient deficiency or meeting out of proportion make growth, physiological metabolism generation obstacle or the ANOMALOUS VARIATIONS of cigarette strain, influence the yield and quality of tobacco leaf.A large amount of and middle amount nutritive element seed soaking is handled has obvious facilitation to seed germination.Trace element at the plant corpus intensive amount seldom, but plant growing is indispensable.Trace element plays regulating action in various degree in the seed germination process.Utilize certain density trace element seed soaking to handle, the enzyme system in can the activated seed body promotes the seed storage substance decomposition, quickens the seed germination process.The MS medium is that Murashige in 1962 and Skoog. design for cultivating tobacco-containing material, contains abundant macroelement, trace element and organic substance, can satisfy a lot of plants at nutrition and physiological needs, and be used widely.Find in this research, carry out the vernalization processing with the MS liquid nutrient medium after, germination rate just can reach 40% in after planting second day, the seed germination time shifts to an earlier date (see figure 3).
4, the influence to the tobacco seed germination rate is handled in the vernalization of complete nutrition liquid
Can find out through above-mentioned experimental result, in the tobacco seed processing procedure, with MS liquid nutrient medium and plant growth regulating substance (GA
3, NAA) separately after the vernalization, can both shorten the seed germination time, and just can produce notable difference with contrast at after planting second day, visible they in this process, all play obvious facilitation.So we are with MS, the GA of variable concentrations
3, NAA carries out combination matching, carries out vernalization more respectively and handles, the statistics tobacco seed is handled later germination rate through different vernalization, is standard with second day germination rate, seeks best vernalization and handles (result sees Fig. 4).The result shows, uses MS+100mg/L GA
3After+2mg/L NAA complete nutrition liquid carried out the vernalization processing, germination rate can reach 84% in after planting second day, apparently higher than handle the seed of handling with independent water vernalization (second day germination rate is respectively 0 and 22%) without vernalization.
5, the vernalization of complete nutrition liquid is to the influence of tobacco seed germinating
Through comparing the influence of different disposal combination, use MS+100mg/L GA to the tobacco seed germination rate
3The vernalization of+2mg/L NAA complete nutrition liquid behaves oneself best result such as Fig. 4 and Fig. 5 after handling.After further observing not vernalization, water vernalization and handle, use up pancebrin vernalization and handle through photomicrograph, the growth conditions in the tobacco seed germination process is handled the influence (Fig. 6) that tobacco seed is sprouted so that observe vernalization intuitively.After planting the 1st day, the seed kind skin of handling without vernalization did not also break, and the seed kind skin that water vernalization and the vernalization of complete nutrition liquid are handled breaks; After planting the 2nd day, the seed kind skin of handling without vernalization did not still break, and the seed that water vernalization is handled is little to be had and show money or valuables one carries unintentionally, and has showed money or valuables one carries unintentionally and the hair that sends out roots and use up pancebrin vernalization seeds treated; After planting the 3rd day, the seed of handling without vernalization just can be observed seed and sprout, and uses up pancebrin vernalization seeds treated, and the growth of root is the fastest, prominent the breaking in the seed coat of its cotyledon and turn green.In general, after vernalization was handled through complete nutrition liquid, tobacco seed not only sprout time can shift to an earlier date, and growth of seedling speed is also compared according to fast simultaneously.
6, the vernalization of complete nutrition liquid is to the influence of tobacco seedling growth
To handle, use up after planting the grow seedling of 14d of pancebrin vernalization seeds treated without vernalization processing, water vernalization and scan, measure the long and hypocotyl length (see figure 7) of its root.Three root length of handling seedling are respectively 0.66cm, 0.88cm and 1.55cm, this shows, the seedling root length after vernalization is handled through complete nutrition liquid is the long twice above (Fig. 8) of contrast root, and has grown lateral root (Fig. 7); Three hypocotyl length of handling seedling are respectively 0.67cm, 0.63cm and 0.96cm, show through the hypocotyl of complete nutrition liquid vernalization processing back seedling also significantly to be longer than contrast (Fig. 9).This explanation is through complete nutrition liquid vernalization seeds treated, and not only sprout time can shift to an earlier date, and the growth of seedling state also obviously is better than the seed without vernalization processing and water vernalization processing simultaneously.
Claims (5)
1. tobacco seed complete nutritional formula germination accelerating method is characterized in that may further comprise the steps:
Tobacco seed is put into the nutritive solution of MS+100mg/L gibberellin+2mg/L methyl, in temperature be 26-28 ℃, 12h illumination/12h is dark and the condition of lasting bubbling air oxygenating under vernalization 42h-46h.
2. the method for claim 1 is characterized in that said method is further comprising the steps of: before presprouting of seeds, put into the weight ratio 0.5% copper-bath 15min that sterilizes, and clean with distilled water flushing again, dry.
3. according to claim 1 or claim 2 method, it is characterized in that: germination temperature is 27 ℃, germination time is 46h.
4. according to claim 1 or claim 2 method, it is characterized in that: during vernalization, seed is suspended in the nutrient solution.
5. according to claim 1 or claim 2 method, it is characterized in that: the tobacco seed after vernalization is handled is processed the tobacco pelleted seed after nucleation, Pelleting, typing, polishing and dyeing.
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Cited By (5)
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| CN104186121A (en) * | 2014-08-08 | 2014-12-10 | 岭南园林股份有限公司 | One-time collecting and sowing method for seeds different in maturation periods |
| CN104429494A (en) * | 2014-11-14 | 2015-03-25 | 何勇 | Dipsacus asperoids rapid seedling method |
| CN106234366A (en) * | 2016-07-28 | 2016-12-21 | 贵州省烟草科学研究院 | A kind of initiation reagent of tobacco seed |
| CN107466522A (en) * | 2017-08-28 | 2017-12-15 | 安徽科技学院 | A kind of vernalization breeding method of sorghum |
| CN109548584A (en) * | 2019-01-16 | 2019-04-02 | 山东农业大学 | A method of promote reed seed to sprout and improve salt tolerance |
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| CN104186121A (en) * | 2014-08-08 | 2014-12-10 | 岭南园林股份有限公司 | One-time collecting and sowing method for seeds different in maturation periods |
| CN104429494A (en) * | 2014-11-14 | 2015-03-25 | 何勇 | Dipsacus asperoids rapid seedling method |
| CN104429494B (en) * | 2014-11-14 | 2016-05-04 | 何勇 | A kind of teasel root fast seedling-cultivating method |
| CN106234366A (en) * | 2016-07-28 | 2016-12-21 | 贵州省烟草科学研究院 | A kind of initiation reagent of tobacco seed |
| CN107466522A (en) * | 2017-08-28 | 2017-12-15 | 安徽科技学院 | A kind of vernalization breeding method of sorghum |
| CN109548584A (en) * | 2019-01-16 | 2019-04-02 | 山东农业大学 | A method of promote reed seed to sprout and improve salt tolerance |
| CN109548584B (en) * | 2019-01-16 | 2021-07-06 | 山东农业大学 | Method for promoting germination of reed seeds and improving salt tolerance |
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Application publication date: 20120418 |