CN109548584A - A method of promote reed seed to sprout and improve salt tolerance - Google Patents
A method of promote reed seed to sprout and improve salt tolerance Download PDFInfo
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- CN109548584A CN109548584A CN201910038644.2A CN201910038644A CN109548584A CN 109548584 A CN109548584 A CN 109548584A CN 201910038644 A CN201910038644 A CN 201910038644A CN 109548584 A CN109548584 A CN 109548584A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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Abstract
The invention discloses a kind of methods that promotion reed seed is sprouted and improves salt tolerance, present invention discover that, after reed seed is handled 12-36h with the gibberellin aqueous solution soaking that concentration is 100mg/L-600mg/L prior to seeding, reed germination percentage greatly improves, speed of germinating is accelerated, and salt tolerance is obviously improved.Low in cost since reed seed source is extensive, gibberellin treatment conditions are simple, therefore, are easy mass disposal.A large amount of high-incidence bud rates, the higher reed seed of salt tolerance are obtained using method of the invention, are very suitable to the Ecological Restoration Project of Saline Soil Area, are also suitable for the Wetland ecological construction in non-salty-soil area, plantation operates also very simple, has broad application prospects.
Description
Technical field
The present invention relates to vegetable seeds processing and ecological restoration technical fields, and in particular to a kind of promotion reed seed sprouting
And the method for improving salt tolerance.
Background technique
Reed is grass family herbaceos perennial, cosmopolitan, in natural habitat, bred with rhizomes based on,
Rhizomes formed in a crisscross manner it is netted, have very strong vitality, can the long period be embedded in underground, once condition be suitable for, still may be used
Shoot is developed into, adapts to different ecological environments extensively.Reed have very strong purification of water quality, brace soil and for animals and plants,
Microorganism provides the Environmental Roles such as living space that live, while reed is in beautification of landscape and farming region ecosystem restoration side
Face is all a kind of very important plant, wide all over the world as the vegetable material of greening shallow water band and riparian buffer strips
General plantation.Reed is also a kind of stronger plant of salt-resistance, is the considerable advantage vegetation of Saline Soil Area, and carbon absorption and solidification energy
Power is much higher than common arbor and shrub.Growth district is difficult to the common vegetation in Saline Soil Area area, carries out artificial growth reed
Vegetation repair accelerates vegetation repair process, both facilitates to alleviate greenhouse effects, regulates the climate, purifies water source, it helps keeps
Ecological diversity.
But the germination rate of reed under field conditions (factors) is very low, generally between 3-5%, cannot achieve effective sowing.
The existing method for carrying out restoration of the ecosystem using reed mainly transplants the rhizomes of mature reed, and transplanting reed needs
A large amount of excavated by manual work seedlings, transport and plantation are wanted, there are problems that at high cost, the death rate is high and destroys local area ecological of collecting seedling.
Summary of the invention
For the above-mentioned prior art, is sprouted the object of the present invention is to provide a kind of promotion reed seed and improve salt tolerance
Method.
To achieve the above object, the present invention adopts the following technical scheme:
The first aspect of the present invention provides gibberellin following 1) -3) at least one of in application:
1) reed seed is promoted to sprout；
2) salt tolerance of reed seed is improved；
3) reed seed is promoted to sprout under condition of salt stress.
Preferably, the concentration of the gibberellin is 100mg/L-600mg/L；It is furthermore preferred that the concentration of the gibberellin is
500mg/L。
Preferably, it is described 3) in, salinity under condition of salt stress is 1-10g/L.
The second aspect of the present invention provides a kind of method that promotion reed seed is sprouted, comprising the following steps:
Reed seed is handled into 12- with the gibberellin aqueous solution soaking that concentration is 100mg/L-600mg/L prior to seeding
36h。
Preferably, the concentration of the gibberellin aqueous solution is 500mg/L, and the immersion treatment time is for 24 hours.
Preferably, the immersion treatment is divided into two stages: soaking under immersion treatment stage and dark condition under illumination condition
Steep processing stage；
The temperature in immersion treatment stage is 26-30 DEG C under the illumination condition, the immersion treatment stage under the dark condition
Temperature be 22-24 DEG C.
The third aspect of the present invention provides a kind of preparation method of reed seed that salt tolerance improves, comprising the following steps:
The reed seed of full grains is chosen, using concentration is the gibberellin aqueous solution of 500mg/L under illumination condition, 28
DEG C immersion treatment 12h, then under dark condition, 24 DEG C of immersion treatment 12h.
The reed seed that the salt tolerance that the above method obtains improves is also protection scope of the present invention.
The fourth aspect of the present invention, provide reed seed that above-mentioned salt tolerance improves in Saline Soil Area restoration of the ecosystem and/or
Application in the construction of non-salty-soil area Wetland ecological.
The fifth aspect of the present invention provides a kind of method sprouted using reed seed and carry out Saline Soil Area restoration of the ecosystem,
The following steps are included:
(1) reed seed treatment: the reed seed of full grains is chosen, uses concentration for the red of 500mg/L prior to seeding
Mycin aqueous solution is under illumination condition, 28 DEG C of immersion treatment 12h, then under dark condition, 24 DEG C of immersion treatment 12h；
(2) sow: by treated, reed seed is sowed in Saline Soil Area, and the buried soil depth of reed seed is 0.5cm-
1cm；
(3) reed seed is sprouted: in reed Their Seed Germinating Period, keep sowing division ground moistening, to reed seed sprout into
The restoration of the ecosystem of row Saline Soil Area.
Beneficial effects of the present invention:
By the present invention in that after handling reed seed under certain condition with the gibberellin aqueous solution of certain concentration, reed
Germination percentage greatly improves, and speed of germinating is accelerated, and salt tolerance is obviously improved.It is low in cost since reed seed source is extensive, it is red
Mycin treatment conditions are simple, therefore, are easy mass disposal.A large amount of high-incidence bud rates, salt tolerance are obtained using method of the invention
Higher reed seed, is very suitable to the Ecological Restoration Project of Saline Soil Area, is also suitable for the Wetland ecological construction in non-salty-soil area,
Plantation operates also very simple, has broad application prospects.
Detailed description of the invention
Fig. 1: various concentration Gibberellins solution adds up germination percentage after causing after impregnating 6,12,18,24,30 hours；It can by Fig. 1
Significantly to observe in each group experiment condition, under conditions of the gibberellin of 500mg/L impregnates 24 hours, reed has highest
Germination percentage.
Fig. 2: growth indexes of the seed impregnated with the red mould solution of 500mg/L under 0-10g/L salinity it
One --- germination index, it can be observed that growth index is a downward trend, even if in salt with the growth of salinity
After concentration reaches 5g/L, still have can preferable growth index, illustrate the processed reed seed salt tolerant of this kind of processing method
Property it is preferable, germination period can expand application range using the irrigation with saline water of 5g/L or so.
Fig. 3: growth indexes of the seed impregnated with the red mould solution of 500mg/L under 0-10g/L salinity it
One --- germination percentage, it can be observed that the processed reed seed of this kind of method still has higher hair under the conditions of the salt of 5g/L
Bud rate.Also further illustrate that the reed seed of such method processing possesses good salt tolerance.
Specific embodiment
It is noted that following detailed description is all illustrative, it is intended to provide further instruction to the application.Unless another
It indicates, all technical and scientific terms used herein has usual with the application person of an ordinary skill in the technical field
The identical meanings of understanding；Wherein:
Germination percentage (GP)=(seed number is planted experimentally in whole chitting piece grain number/confessions) × 100%；
Germinating energy (GE)=(chitting piece number/seed sum in alloted days) × 100%；
Germination index (Gi)=∑ Gt/Dt, Gt is the germinative number in the t time, and Dt is corresponding germinating time (d).
As background technology part introduction, the germination rate of reed seed under field conditions (factors) is very low, and only 3-5% is left
The right side cannot achieve effective sowing.
Gibberellin (GA) is a kind of dynamical broad-spectrum plant growth regulator, and most significant physiological effect is to promote to plant
The growth and development of object is mainly completed by the increase of cell number and cell elongation, is widely used in agricultural production.
Although about gibberellin alleviate salt stress to seed sprout and growth of seedling influence on rice, tomato, capsicum, red sand etc.
It has been reported that.But due to the size of different vegetable seeds, the kind differences such as transdermally, different plant seed germinations are to seed soaking
The response of pretreatment mode is different, and applicable drug processing mode is also different.
For reed seed, since seed is too small, kind transdermally is bad, germination rate under field conditions (factors) is just very
Low, if germination period is influenced by salt stress again, reed seed basically can not be sprouted.Therefore, how to reed seed
It is pre-processed, to improve the germination rate of reed seed, especially germination percentage of the raising reed seed under condition of salt stress, one
It is directly the difficult point place of this field.
To promote reed seed to sprout and improve its salt tolerance, the present invention selects to impregnate reed seed with gibberellin
Processing counts germination percentage respectively with after the gibberellin aqueous solution soaking 6 of various concentration, 12,18,24,30h respectively.As a result as schemed
Shown in 1.
As seen from Figure 1, for 24 hours using the gibberellin aqueous solution soaking of 500mg/L, the germination percentage highest of reed seed.
The environmental factors such as illumination, temperature can also have an impact reed seed sprouting, but specifically how influence, at present still
It completely contradicts without consistent cognition, or even the conclusion that different researchs obtain.Gibberellin concentration of aqueous solution and immersion after optimization
On the basis of time, the present invention has further investigated illumination and temperature condition sprouts reed seed influence.Through test of many times
It was found that using concentration for the gibberellin aqueous solution of 500mg/L under illumination condition, 28 DEG C of immersion treatments in reed seed
12h, then under dark condition, 24 DEG C of immersion treatment 12h；Its treatment effect is optimal, under above-mentioned pretreatment condition, reed seed
Germination percentage reach as high as 100%.
For the salt tolerance for further investigating the reed seed after gibberellin immersion treatment, the present invention has carried out salt tolerance hair
Bud test, chooses 1100 reed seeds after gibberellin immersion treatment, and every 100 are placed in a culture dish, respectively with 1,
2,3,4,5,6,7,8,9, the salting liquid of 10g/ml is cultivated, using the clear water of equivalent as blank control (CK).
It is recorded every 12h, continuous record 10 days calculates germination index and germination percentage, as a result sees Fig. 2 and Fig. 3 respectively.
As seen from Figure 2, with the growth of salinity, growth index is a downward trend, even if dense in salt
Degree reach 5g/L after, still have can preferable growth index, illustrate the processed reed seed salt tolerance of this kind of processing method
Preferably, germination period can expand application range using the irrigation with saline water of 5g/L or so.
As seen from Figure 3, under the conditions of the salt of 5g/L, still had using the processed reed seed of the method for the present invention higher
Germination percentage.Also further illustrate that the reed seed of such method processing possesses good salt tolerance.
To sum up, using method of the invention, seed sprouting is made to be significantly increased in germination percentage, germinating energy, germination index, and
In 500mg/L gibberellin, soaking time is to deposit on germination percentage, germinating energy, germination index under the conditions of 24 hours with conventional seed
In significant difference, almost close to 100% germination percentage.And salt tolerant sex expression is good.Operation of the present invention is simple, low in cost, can
To be widely used.By this method, treated that germination is fast, and salt tolerance significantly increases, before having the application of good market
Scape.
In order to enable those skilled in the art can clearly understand the technical solution of the application, below with reference to tool
The technical solution of the application is described in detail in the embodiment of body.
Test material used in the embodiment of the present invention is the test material of this field routine, can pass through commercial channel
It is commercially available.
Embodiment 1:
The reed seed of full grains is chosen, using concentration is the gibberellin aqueous solution of 500mg/L under illumination condition, 28
DEG C immersion treatment 12h, then under dark condition, 24 DEG C of immersion treatment 12h；The reed seed that obtains that treated.
Embodiment 2:
The reed seed of full grains is chosen, using concentration is the gibberellin aqueous solution of 400mg/L under illumination condition, 28
DEG C immersion treatment 6h, then under dark condition, 22 DEG C of immersion treatment 6h；The reed seed that obtains that treated.
Embodiment 3:
The reed seed of full grains is chosen, using concentration is the gibberellin aqueous solution of 600mg/L under illumination condition, 28
DEG C immersion treatment 9h, then under dark condition, 24 DEG C of immersion treatment 9h；The reed seed that obtains that treated.
Comparative example 1:
The reed seed of full grains is chosen, using concentration is the gibberellin aqueous solution of 500mg/L under illumination condition, 28
DEG C immersion treatment 6h, then under dark condition, 24 DEG C of immersion treatment 6h；The reed seed that obtains that treated.
Comparative example 2:
The reed seed of full grains is chosen, using concentration is the gibberellin aqueous solution of 200mg/L under illumination condition, 28
DEG C immersion treatment 6h, then under dark condition, 24 DEG C of immersion treatment 6h；The reed seed that obtains that treated.
Comparative example 3:
The reed seed of full grains is chosen, using concentration is the gibberellin aqueous solution of 100mg/L under illumination condition, 28
DEG C immersion treatment 12h, then under dark condition, 24 DEG C of immersion treatment 12h；The reed seed that obtains that treated.
Comparative example 4:
The reed seed of full grains is chosen, using concentration is the gibberellin aqueous solution of 300mg/L under illumination condition, 28
DEG C immersion treatment 12h, then under dark condition, 24 DEG C of immersion treatment 12h；The reed seed that obtains that treated.
Test example 1:
1. test method:
100 embodiment 1- embodiments 3, comparative example 1- comparative example 4 are taken respectively treated reed seed is placed in culture dish
It is interior, distilled water is added, makes water level depth 1cm, at identical temperature (20 DEG C) and illumination condition (12h illumination, 12h are dark)
It is cultivated.
Using untreated reed seed as blank control.
The same time observes germination situation and records germinative number daily, continuous record 10 days.Calculate the hair in 10 days
Bud rate and the 36th hour germinating energy.
2. test result:
The calculated result of each group reed percentage of seedgermination and the 36th hour germinating energy is shown in Table 1.
Table 1: germination percentage and germinating energy calculated result
Test example 2:
1. test method:
100 embodiments 1 are taken respectively treated that reed seed is placed in culture dish, repeat five times, be respectively placed in five
A culture dish, and be separately added into and concentration 1g/L, 3g/L, 5g/L is added, the sodium chloride solution of 7g/L, 9g/L make water level depth
1cm is cultivated at identical temperature (20 DEG C) and illumination condition (12h illumination, 12h are dark).
Using untreated reed seed as blank control.
The same time observes germination situation and records germinative number daily, continuous record 10 days.Calculate the hair in 10 days
Bud rate and the 36th hour germinating energy.
2. test result:
The calculated result of each group reed percentage of seedgermination and the 36th hour germinating energy is shown in Table 2.
Table 2: germination percentage and germinating energy calculated result
| Group (salinity) | Germination percentage (%) | Germinating energy (%) |
| 1g/L | 89 | 45 |
| 3g/L | 68 | 19 |
| 5g/L | 49 | 17 |
| 7g/L | 25 | 2 |
| 9g/L | 12 | 0 |
| Blank control+1g/L | 5 | 2 |
| Blank control+3g/L | 2 | 2 |
| Blank control+5g/L | 0 | 0 |
| Blank control+7g/L | 0 | 0 |
| Blank control+9g/L | 0 | 0 |
The foregoing is merely preferred embodiment of the present application, are not intended to limit this application, for the skill of this field
For art personnel, various changes and changes are possible in this application.Within the spirit and principles of this application, made any to repair
Change, equivalent replacement, improvement etc., should be included within the scope of protection of this application.
Claims (10)
1. gibberellin is following 1) -3) at least one of in application:
1) reed seed is promoted to sprout；
2) salt tolerance of reed seed is improved；
3) reed seed is promoted to sprout under condition of salt stress.
2. application according to claim 1, which is characterized in that the concentration of the gibberellin is 100mg/L-600mg/L；It is excellent
Choosing, the concentration of the gibberellin is 500mg/L.
3. application according to claim 1, which is characterized in that it is described 3) in, salinity under condition of salt stress is 1-
10g/L。
4. a kind of method for promoting reed seed to sprout, which comprises the following steps:
Reed seed is handled into 12-30h with the gibberellin aqueous solution soaking that concentration is 100mg/L-600mg/L prior to seeding.
5. according to the method described in claim 4, it is characterized in that, the concentration of the gibberellin aqueous solution is 500mg/L, immersion
Handling the time is for 24 hours.
6. method according to claim 4 or 5, which is characterized in that the immersion treatment is divided into two stages: illumination condition
The immersion treatment stage under lower immersion treatment stage and dark condition；
The temperature in immersion treatment stage is 26-30 DEG C under the illumination condition, the temperature in immersion treatment stage under the dark condition
Degree is 22-24 DEG C.
7. a kind of preparation method for the reed seed that salt tolerance improves, which comprises the following steps:
The reed seed for choosing full grains uses concentration for the gibberellin aqueous solution of 500mg/L under illumination condition, 28 DEG C of leachings
Bubble processing 12h, then under dark condition, 24 DEG C of immersion treatment 12h.
8. the reed seed that the salt tolerance that method obtains according to claim 7 improves.
9. the reed seed that salt tolerance according to any one of claims 8 improves is wet in Saline Soil Area restoration of the ecosystem and/or non-salty-soil area
Application in ground ecological construction.
10. a kind of sprout the method for carrying out Saline Soil Area restoration of the ecosystem using reed seed, which is characterized in that including following step
It is rapid:
(1) reed seed treatment: the reed seed of full grains is chosen, uses concentration for the gibberellin of 500mg/L prior to seeding
Aqueous solution is under illumination condition, 28 DEG C of immersion treatment 12h, then under dark condition, 24 DEG C of immersion treatment 12h；
(2) sow: by treated, reed seed is sowed in Saline Soil Area, and the buried soil depth of reed seed is 0.5cm-1cm；
(3) reed seed is sprouted: in reed Their Seed Germinating Period, being kept sowing division ground moistening, is sprouted to reed seed and carry out salt
The restoration of the ecosystem of the area Zi Tu.
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| CN111642337A (en) * | 2020-07-04 | 2020-09-11 | 江西省林业科学院 | Method for restoring degraded wetland at tail of reservoir based on reed vegetation |
| CN112567925A (en) * | 2020-12-11 | 2021-03-30 | 菏泽学院 | Method for improving salt stress resistance of lawn grass germination by gibberellin seed soaking |
| US11310975B2 (en) * | 2019-04-23 | 2022-04-26 | 4D Bios Inc. | Method for cultivating Mesembryanthemum crystallinum |
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