CN107155444A - A kind of method for promoting Whitebackleaf Mallotus Root seed to sprout - Google Patents
A kind of method for promoting Whitebackleaf Mallotus Root seed to sprout Download PDFInfo
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- CN107155444A CN107155444A CN201710330793.7A CN201710330793A CN107155444A CN 107155444 A CN107155444 A CN 107155444A CN 201710330793 A CN201710330793 A CN 201710330793A CN 107155444 A CN107155444 A CN 107155444A
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- seed
- mallotus root
- whitebackleaf mallotus
- root seed
- whitebackleaf
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
A kind of method for promoting Whitebackleaf Mallotus Root seed to sprout, this method comprises the steps：Whitebackleaf Mallotus Root seed is put into distilled water, and placed 4 hours at 20 DEG C；The Whitebackleaf Mallotus Root seed soaked is taken out, the removal loose oil-containing structure of surface black is washed by rubbing with the hands, is rinsed well with clear water, and blotted the surface of the seed moisture with blotting paper.Treated seed is seeded on 1% agar medium containing 200 mg/litre gibberellin, culture medium is positioned at 20 DEG C and sprouted, intensity of illumination is 1000 luxs, photoperiod is 12 hours illumination/12 hour dark, using the inventive method, the germination rate of Whitebackleaf Mallotus Root seed can be brought up to close to 66.7% by 30%.The processing method is simple and easy to do, can effectively facilitate the sprouting of Whitebackleaf Mallotus Root seed, and without any dangerous and toxic side effect.
Description
Technical field
The invention belongs to plant seed germination technical field, in particular it relates to a kind of side for promoting Whitebackleaf Mallotus Root seed to sprout
Method.
Background technology
Whitebackleaf Mallotus Root (Mallotus apelta (Lour.) Muell.Arg.) is Euphorbiaceae (Euphorbiaceae) Mallotus
(Mallotus Lour.) machaka or dungarunga seeds.It is frequently grown the roadside in southern each province level land hills and barren drying
Guanshan Mountain or deserted mountain on, adaptability is especially strong, available for preventing soil loss and ecological recovery.Spring tender leaf can blend other feedings
Material pig feeding, winter collects leaf compost.Stem skin is fibrous material, weaves a gunnysack, is made for blending or makees paraffin paper and synthetic cotton
Raw material.Root, the leaf of Whitebackleaf Mallotus Root are used as medicine；Leaf has effects that clearing heat and promoting diuresis, analgesic antidotal and hemostasis, available for treatment tympanitis,
Aphtha, traumatic injury, eczema, traumatism and bleeding etc.；Root has soft liver promoting blood circulation, invigorating the spleen for eliminating dampness, the effect of astringing and avoiding visceroptosis, available for controlling
Treat the illnesss such as chronic hepatitis, hepatosplenomegaly, oedema；Treatment stomachache is also used for simultaneously to vomit the diseases such as water, traumatism and bleeding and skin pruritus.
Seed oil content is up to 36%, containing α-Mallotus philippinensis acid, is available for the raw materials such as liquefaction paint, or the big ring spices of synthesis, bactericide, lubricant,
Development volue has a high potential.Whitebackleaf Mallotus Root is although distributed more widely in China, but stock number is less, Whitebackleaf Mallotus Root either cuttage
Or epicormic branch cuttage, its reproductive survival rate is not high, only 30% or so.At present, Whitebackleaf Mallotus Root cultivation technique is ripe not enough, not
See that scale breeding reports that sowing is still one of main reproduction technique.
At present, in the prior art, the germination rate of Whitebackleaf Mallotus Root fresh seeds is about 30%-50%.
The content of the invention
It is an object of the invention to provide a kind of method that simple and effective sprouts Whitebackleaf Mallotus Root seed, to improve Whitebackleaf Mallotus Root seed
Germination rate.The germination rate of Whitebackleaf Mallotus Root seed by 30% can have been brought up to 66.7% by the method for the present invention, simple and easy to do, without appointing
What dangerous and toxic side effect.
In order to realize the above-mentioned purpose of the present invention, the invention provides following technical scheme：
A kind of method that Whitebackleaf Mallotus Root seed is sprouted after promotion Cord blood, this method comprises the steps：
(1) seed is put into distilled water, and placed 4 hours at 20 DEG C；The seed soaked is taken out, removal is washed by rubbing with the hands
The loose oil-containing structure of surface black, is rinsed well, and blotted the surface of the seed moisture with blotting paper with clear water.
(2) seed for treating step (1) is seeded on 1% agar medium containing 200 mg/litre gibberellin, will
Culture medium, which is positioned at 20 DEG C, to be sprouted, and intensity of illumination is 1000 luxs, and the photoperiod is 12 hours illumination/12 hour dark,
Embodiment：
The essentiality content of the present invention is further illustrated with the embodiment of the present invention below, but this hair is not limited with this
It is bright.
Embodiment 1：
1. material and method：
1.1 research material：
In October, 2015, the ripe fresh Whitebackleaf Mallotus Root seed of Honghe state, Yunnan Province Yuanyang County Guanyin Mountain collection.Transported after collection
Return Kunming Institute of Zoology Southwest China wildlife germplasm resource bank Seed Deposit center preservation.Storage conditions are 15 DEG C,
15% relative humidity.Holding time is 10 months.
1.2 research method：
1.2.1 experimental design：Seed after preservation is directly taken out.It is divided into 4 processing, respectively control group, washes group by rubbing with the hands,
Group is washed by rubbing with the hands after washing group and dioxygen water process by rubbing with the hands after distillation water process.Wherein wash by rubbing with the hands and rubbed with the hands after group and dioxygen water process after distillation water process
Wash group and respectively set 3 gradients, respectively immersion 4,8 and 16 hours.Each 3 repetitions of processing, each repeat 40 seeds.
1.2.2 water process is distilled：360 seeds are put into 3 35ml vials with cover, 120 every bottle, are separately added into
Distilled water 20ml, tightens lid and is positioned in 20 DEG C of illumination boxs.120 kinds are taken out at random successively 4, after 8 and 16 hours
Son, is gently washed by rubbing with the hands on gauze, is removed the loose oil-containing structure of surface black, is rinsed well with running water, and will be planted with blotting paper
Sub- surface moisture is blotted.
1.2.3 dioxygen water process：360 seeds are put into 3 35ml vials with cover, 120 every bottle, are separately added into
3-3.5% hydrogen peroxide 20ml, tightens lid and is positioned in 20 DEG C of illumination boxs.Taken at random successively 4, after 8 and 16 hours
Go out 120 seeds, gently washed by rubbing with the hands on gauze, remove the loose oil-containing structure of surface black, rinsed well with running water, and with suction
Water paper blots the surface of the seed moisture.
1.2.4 processing is washed by rubbing with the hands：By seed with gently being washed by rubbing with the hands on gauze immediately after water-soaked, remove that surface black is loose to be contained
Oily structure, is rinsed well with running water, and is blotted the surface of the seed moisture with blotting paper.
1.2.5 sprout：This experiment is using 2 kinds of culture mediums, and one kind is common 1% agar medium, and another is to contain 200
1% agar medium of milligrams per liter gibberellin.Seed is seeded in respectively on 2 kinds of culture mediums, is fitted into transparent valve bag and prevents
In only moisture loss, the illumination box for being subsequently placed in 20 DEG C, intensity of illumination is 1000 luxs, and the photoperiod was 12 small time
According to/12 hours dark.Checked and once sprout at interval of 2-3 days, radicle extends over 5mm and thinks that seed is sprouted, and will sprout and plant
Son takes out, and records sprouting quantity；Terminate experiment without sprouting within continuous 2 weeks after experiment starts 4 weeks.
1.2.5 data analysis：Analyzed sprouting data, entered using One-Way ANOVA using SPSS16.0 softwares
Row variance analysis, and Multiple range test is done to the germination rate of different disposal with S-N-K methods.
2. result is with discussing
The influence that 2.1 different disposal methods are sprouted to Whitebackleaf Mallotus Root seed：
Influence of the different disposal of table 1 to Whitebackleaf Mallotus Root seed germination rate (%)
Note：Numeral is average value ± standard error, between different letter expression different disposals on the culture medium containing gibberellin
Germination rate there is significant difference.
As can be seen from Table 1, on the ordinary culture medium without gibberellin, seed can not be sprouted.Undressed seed
Germination rate on the agar medium containing 200mg/L gibberellin is 30%, and distilled water immersion washes the kind of processing by rubbing with the hands after 4 hours
Sub- germination rate is up to 66.7%, and effect is best in all processing；The effect washed by rubbing with the hands and distilled water immersion 4 hours are without significance difference
It is different, but the loose oil-containing structure of outer covering of the seed black is more easily removed after immersion.
3rd, the positive effect of the present invention is as follows：The sprouting of Whitebackleaf Mallotus Root seed is significantly promoted, by the sprouting of Whitebackleaf Mallotus Root seed
Rate has brought up to 66.7% by 30%, and the processing method is simple and easy to do, without any dangerous and toxic side effect.
Claims (1)
1. a kind of method for promoting Whitebackleaf Mallotus Root seed to sprout, it is characterised in that：This method comprises the steps：
(1) Whitebackleaf Mallotus Root seed is put into distilled water, and placed 4 hours at 20 DEG C；4 as a child take out seed, wash removal by rubbing with the hands
The loose oil-containing structure of surface black, is rinsed well, and blotted the surface of the seed moisture with blotting paper with clear water；
(2) seed for treating step (1) is seeded on 1% agar medium containing 200 mg/litre gibberellin, will be cultivated
Base, which is positioned at 20 DEG C, to be sprouted, and intensity of illumination is 1000 luxs, and the photoperiod is 12 hours illumination/12 hour dark.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710330793.7A CN107155444A (en) | 2017-05-11 | 2017-05-11 | A kind of method for promoting Whitebackleaf Mallotus Root seed to sprout |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710330793.7A CN107155444A (en) | 2017-05-11 | 2017-05-11 | A kind of method for promoting Whitebackleaf Mallotus Root seed to sprout |
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| Publication Number | Publication Date |
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| CN107155444A true CN107155444A (en) | 2017-09-15 |
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| CN201710330793.7A Pending CN107155444A (en) | 2017-05-11 | 2017-05-11 | A kind of method for promoting Whitebackleaf Mallotus Root seed to sprout |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109548584A (en) * | 2019-01-16 | 2019-04-02 | 山东农业大学 | A method of promote reed seed to sprout and improve salt tolerance |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6905876B2 (en) * | 2001-11-16 | 2005-06-14 | Council Of Scientific And Industrial Research | Methods and compositions for in vitro germination and propagation of polygonatum cirrhifolium royle |
| CN104782495A (en) * | 2015-05-02 | 2015-07-22 | 冯文杰 | Tissue culture and rapid propagation method for euphorbia lathyris |
| CN105638470A (en) * | 2016-01-09 | 2016-06-08 | 佛山市金蓝领教育科技有限公司 | Ginseng seed germination culture medium |
-
2017
- 2017-05-11 CN CN201710330793.7A patent/CN107155444A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6905876B2 (en) * | 2001-11-16 | 2005-06-14 | Council Of Scientific And Industrial Research | Methods and compositions for in vitro germination and propagation of polygonatum cirrhifolium royle |
| CN104782495A (en) * | 2015-05-02 | 2015-07-22 | 冯文杰 | Tissue culture and rapid propagation method for euphorbia lathyris |
| CN105638470A (en) * | 2016-01-09 | 2016-06-08 | 佛山市金蓝领教育科技有限公司 | Ginseng seed germination culture medium |
Non-Patent Citations (2)
| Title |
|---|
| 周剑等: "《植物学》", 30 September 2015, 延边大学出版社 * |
| 陈世昌等: "《植物组织培养》", 30 June 2016 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109548584A (en) * | 2019-01-16 | 2019-04-02 | 山东农业大学 | A method of promote reed seed to sprout and improve salt tolerance |
| CN109548584B (en) * | 2019-01-16 | 2021-07-06 | 山东农业大学 | Method for promoting germination of reed seeds and improving salt tolerance |
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Application publication date: 20170915 |
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