CN105638470A - Ginseng seed germination culture medium - Google Patents
Ginseng seed germination culture medium Download PDFInfo
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- CN105638470A CN105638470A CN201610011307.0A CN201610011307A CN105638470A CN 105638470 A CN105638470 A CN 105638470A CN 201610011307 A CN201610011307 A CN 201610011307A CN 105638470 A CN105638470 A CN 105638470A
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- ginseng seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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Abstract
The invention provides a ginseng seed germination culture medium and belongs to the technical field of ginseng tissue culture. KT (kinetin), NAA (Naphthalene Acetic Acid), GA3 (gibberellin), malt extract, agar powder and white granulated sugar are added; the malt extract contains a plurality of types of natural hormones and enzymes, and the seed dormancy can be broken. The ginseng seed germination culture medium provided by the invention has the advantages of high germination rate and short germination time.
Description
Technical field
The invention belongs to ginseng tissue culture technique field, particularly to a kind of ginseng seed's germination medium.
Background technology
Radix Ginseng is Angiospermae Dicotyledoneae Archichlamydeae Araliaceae, herbaceos perennial, it is grown on thick forest, it is distributed in Heilungkiang, Jilin, Liaoning and northern Hebei remote mountains more, containing more than 10 kinds of ginsenosides, and the fast alcohol of Radix Ginseng, several amino acids and vitamin, it is famous and precious Chinese medicinal herbs.
But the material of a large amount of Inhibiting germination after ginseng seed's maturation, can be accumulated, stop ginseng seed to sprout, make ginseng seed's penetration depth dormancy, cause Radix Ginseng aseptic seeding germination rate extremely low, it is difficult to meet the demand of market and scientific research.
Summary of the invention
There are the problems referred to above based on prior art, the present invention provides a kind of ginseng seed's germination medium, it also adds KT(kinetins), NAA(naphthalene acetic acid), GA3(gibberellins), beerwort, agar powder and white sugar, containing multiple natural hormone and enzyme in beerwort, seed dormancy can be broken, ginseng seed's germination medium provided by the invention has germination rate height, the advantage that sprout time is short.
A kind of ginseng seed's germination medium, it is characterized in that, it is culture medium based on MS culture medium, and add KT(kinetins), NAA(naphthalene acetic acid), GA3(gibberellins), beerwort, agar powder and white sugar, containing multiple natural hormone and enzyme in beerwort, can breaking seed dormancy, sprouting is had facilitation by GA3.
KT(kinetins) concentration be 0.5-2mg/L, NAA(naphthalene acetic acid) concentration be 0.1-0.4mg/L, GA3(gibberellins) concentration be 0.05-0.2mg/L, beerwort concentration be 100-200g/L, the concentration of agar powder be 8-12g/L, white sugar concentration be 25-35g/L.
KT(kinetins) concentration be 0.8-1.2mg/L, NAA(naphthalene acetic acid) concentration be 0.2-0.3mg/L, GA3(gibberellins) concentration be 0.08-0.12mg/L, beerwort concentration be 130-180g/L, the concentration of agar powder be 9-10g/L, white sugar concentration be 28-32g/L.
The pH value of described rapid induction ginseng callus culture medium is 5.8-6.8.
The pH value of described rapid induction ginseng callus culture medium is 6.3.
A kind of compound method of ginseng seed's germination medium, it comprises the following steps:
Step S10 dissolves preparation culture medium: dissolves each composition of MS basal medium with distilled water, and adds KT(kinetins), NAA(naphthalene acetic acid), GA3(gibberellins), beerwort, agar powder and white sugar;
Step S20 high temperature sterilize: the culture medium prepared by step S10 is put in high-pressure sterilizing pot, in 120 ~ 121 DEG C of high temperature sterilizes 20 ~ 21 minutes, cooled and solidified.
Step S10 also includes step S11 and regulates pH value: use HCL and KOH titration to regulate Medium's PH Value to 6.3.
Detailed description of the invention
Below in conjunction with specific embodiment, the invention will be further described.
Embodiment one: preparation ginseng seed's germination medium.
MS culture medium prescription according to table 1 and the ginseng seed's germination medium formula shown in table 2, prepare ginseng seed's germination medium of the present invention.
Weigh each composition in MS culture medium and KT, NAA, GA3, beerwort, agar powder and white sugar with balance respectively, be placed in triangular flask, add appropriate purified water, stirring and dissolving, be settled to 1000mL, and regulate pH value to 6.3. Triangular flask is sealed, is placed in high-pressure sterilizing pot, in 121 DEG C of sterilizing 21min, then in superclean bench, by ginseng seed's germination medium subpackage to tissue culture flasks, natural cooling, by tissue culture's bottle closure after its solidification, standby.
The composition of table 1MS culture medium and consumption thereof.
The composition of table 2 ginseng seed's germination medium and consumption thereof.
Embodiment two: the test of ginseng seed's germination medium of the present invention.
Ginseng seed's germination medium is prepared by the method described in embodiment one, standby.
Take the ripe ginseng seed of full grains, ginseng seed is carried out soaking 20 hours by the cold water using 10 DEG C, take out seed, and by seed mass concentration is 75% alcohol-pickled 30 seconds in superclean bench, with aseptic water washing 4 times, sterilize 6 minutes with mercuric chloride solution that mass concentration is 0.12% again, then with aseptic water washing 4 times.
By sterilizing, complete ginseng seed is inoculated in the above-mentioned ginseng seed's germination medium prepared, and put into cultivation in growth cabinet, condition of culture is light dark period is that 14h illumination/12h is dark, cultivate when intensity of illumination is 2000-2300lux, first seed germination is begun with when cultivating by the 17th day, grow up to Radix Ginseng aseptic seedling, after cultivating 22 days, ginseng seed more than 90% sprouts, induce into aseptic seedling, seed germination rate is up to 90%, higher than the germination rate of conventional abductive approach, the conventional average sprout time of abductive approach is after 25 days, use ginseng seed's germination medium provided by the invention can shorten induction time.
Claims (7)
1. ginseng seed's germination medium, it is characterised in that it is culture medium based on MS culture medium, and add KT(kinetins), NAA(naphthalene acetic acid), GA3(gibberellins), beerwort, agar powder and white sugar.
2. a kind of ginseng seed's germination medium according to claim 1, it is characterized in that, KT(kinetins) concentration be 0.5-2mg/L, NAA(naphthalene acetic acid) concentration be 0.1-0.4mg/L, GA3(gibberellins) concentration be 0.05-0.2mg/L, beerwort concentration be 100-200g/L, the concentration of agar powder be 8-12g/L, white sugar concentration be 25-35g/L.
3. a kind of ginseng seed's germination medium according to claim 1, it is characterized in that, KT(kinetins) concentration be 0.8-1.2mg/L, NAA(naphthalene acetic acid) concentration be 0.2-0.3mg/L, GA3(gibberellins) concentration be 0.08-0.12mg/L, beerwort concentration be 130-180g/L, the concentration of agar powder be 9-10g/L, white sugar concentration be 28-32g/L.
4. according to one of them described a kind of ginseng seed's germination medium of claims 1 to 3, it is characterised in that the pH value of described rapid induction ginseng callus culture medium is 5.8-6.8.
5. according to one of them described a kind of ginseng seed's germination medium of claims 1 to 3, it is characterised in that the pH value of described rapid induction ginseng callus culture medium is 6.3.
6. the compound method of ginseng seed's germination medium, it is characterised in that it comprises the following steps:
Step S10 dissolves preparation culture medium: dissolves each composition of MS basal medium with distilled water, and adds KT(kinetins), NAA(naphthalene acetic acid), GA3(gibberellins), beerwort, agar powder and white sugar;
Step S20 high temperature sterilize: the culture medium prepared by step S10 is put in high-pressure sterilizing pot, in 120 ~ 121 DEG C of high temperature sterilizes 20 ~ 21 minutes, cooled and solidified.
7. the compound method of a kind of ginseng seed's germination medium according to claim 7, it is characterised in that described step S10 also includes step S11 and regulates pH value: use HCL and KOH titration to regulate Medium's PH Value to 6.3.
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| CN201610011307.0A CN105638470A (en) | 2016-01-09 | 2016-01-09 | Ginseng seed germination culture medium |
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| CN201610011307.0A CN105638470A (en) | 2016-01-09 | 2016-01-09 | Ginseng seed germination culture medium |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106035416A (en) * | 2016-06-29 | 2016-10-26 | 固镇县华原家庭农场 | Germination accelerating agent for effectively increasing germination rate of chili seeds |
| CN107155444A (en) * | 2017-05-11 | 2017-09-15 | 中国科学院昆明植物研究所 | A kind of method for promoting Whitebackleaf Mallotus Root seed to sprout |
| CN108887083A (en) * | 2018-07-26 | 2018-11-27 | 合肥仙之峰农业科技有限公司 | A kind of implantation methods of oyster mushroom |
Citations (4)
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| CN101156553A (en) * | 2007-11-16 | 2008-04-09 | 中国科学院新疆理化技术研究所 | a method for rapid evoking adventive bud panax ginseng stem segment |
| CN102657095A (en) * | 2012-05-25 | 2012-09-12 | 东北师范大学 | Efficient tissue culture and regeneration method for ginseng |
| CN104472359A (en) * | 2014-11-26 | 2015-04-01 | 中国医学科学院药用植物研究所 | Ginseng adventitious root induced proliferation method |
| CN104721359A (en) * | 2013-12-19 | 2015-06-24 | 青岛蓝图文化传播有限公司市南分公司 | Chinese medicinal composition for invigorating qi and blood |
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2016
- 2016-01-09 CN CN201610011307.0A patent/CN105638470A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101156553A (en) * | 2007-11-16 | 2008-04-09 | 中国科学院新疆理化技术研究所 | a method for rapid evoking adventive bud panax ginseng stem segment |
| CN102657095A (en) * | 2012-05-25 | 2012-09-12 | 东北师范大学 | Efficient tissue culture and regeneration method for ginseng |
| CN104721359A (en) * | 2013-12-19 | 2015-06-24 | 青岛蓝图文化传播有限公司市南分公司 | Chinese medicinal composition for invigorating qi and blood |
| CN104472359A (en) * | 2014-11-26 | 2015-04-01 | 中国医学科学院药用植物研究所 | Ginseng adventitious root induced proliferation method |
Non-Patent Citations (2)
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106035416A (en) * | 2016-06-29 | 2016-10-26 | 固镇县华原家庭农场 | Germination accelerating agent for effectively increasing germination rate of chili seeds |
| CN107155444A (en) * | 2017-05-11 | 2017-09-15 | 中国科学院昆明植物研究所 | A kind of method for promoting Whitebackleaf Mallotus Root seed to sprout |
| CN108887083A (en) * | 2018-07-26 | 2018-11-27 | 合肥仙之峰农业科技有限公司 | A kind of implantation methods of oyster mushroom |
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