AU2020101701A4 - Culture medium for promoting the elongation of clump buds of vaccinium bracteatum thunb, a preparation method and an application thereof - Google Patents
Culture medium for promoting the elongation of clump buds of vaccinium bracteatum thunb, a preparation method and an application thereof Download PDFInfo
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- 239000001963 growth medium Substances 0.000 title claims abstract description 82
- 244000287839 Vaccinium bracteatum Species 0.000 title claims abstract description 38
- 235000005480 Vaccinium bracteatum Nutrition 0.000 title claims abstract description 38
- 230000001737 promoting effect Effects 0.000 title claims abstract description 20
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 244000061456 Solanum tuberosum Species 0.000 claims abstract description 40
- 235000002595 Solanum tuberosum Nutrition 0.000 claims abstract description 40
- 229920001817 Agar Polymers 0.000 claims abstract description 35
- 239000008272 agar Substances 0.000 claims abstract description 35
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 27
- 229930006000 Sucrose Natural products 0.000 claims abstract description 27
- 239000005720 sucrose Substances 0.000 claims abstract description 27
- 239000005648 plant growth regulator Substances 0.000 claims abstract description 8
- 239000000843 powder Substances 0.000 claims description 24
- 239000007864 aqueous solution Substances 0.000 claims description 22
- 239000000203 mixture Substances 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- 238000005520 cutting process Methods 0.000 claims description 2
- 238000010438 heat treatment Methods 0.000 claims description 2
- 239000000155 melt Substances 0.000 claims description 2
- 238000007789 sealing Methods 0.000 claims description 2
- 238000005303 weighing Methods 0.000 claims description 2
- 230000012010 growth Effects 0.000 abstract description 7
- 235000015097 nutrients Nutrition 0.000 abstract description 4
- 238000004161 plant tissue culture Methods 0.000 abstract description 4
- 150000003839 salts Chemical class 0.000 abstract description 3
- 229930192334 Auxin Natural products 0.000 abstract description 2
- 229920002472 Starch Polymers 0.000 abstract description 2
- 150000001413 amino acids Chemical class 0.000 abstract description 2
- 239000002363 auxin Substances 0.000 abstract description 2
- 239000000835 fiber Substances 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 244000005700 microbiome Species 0.000 abstract description 2
- 102000004169 proteins and genes Human genes 0.000 abstract description 2
- 108090000623 proteins and genes Proteins 0.000 abstract description 2
- 239000008107 starch Substances 0.000 abstract description 2
- 235000019698 starch Nutrition 0.000 abstract description 2
- 230000009286 beneficial effect Effects 0.000 abstract 1
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 18
- 230000000052 comparative effect Effects 0.000 description 13
- 239000002609 medium Substances 0.000 description 9
- 239000000243 solution Substances 0.000 description 7
- 230000018109 developmental process Effects 0.000 description 4
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 4
- 235000012015 potatoes Nutrition 0.000 description 4
- 235000013399 edible fruits Nutrition 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 241000371652 Curvularia clavata Species 0.000 description 1
- 238000012270 DNA recombination Methods 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000208421 Ericaceae Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 235000012511 Vaccinium Nutrition 0.000 description 1
- 235000021028 berry Nutrition 0.000 description 1
- 230000007910 cell fusion Effects 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000012214 genetic breeding Methods 0.000 description 1
- 238000012248 genetic selection Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000007226 seed germination Effects 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- UZKQTCBAMSWPJD-UQCOIBPSSA-N trans-Zeatin Natural products OCC(/C)=C\CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-UQCOIBPSSA-N 0.000 description 1
- UZKQTCBAMSWPJD-FARCUNLSSA-N trans-zeatin Chemical compound OCC(/C)=C/CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-FARCUNLSSA-N 0.000 description 1
- 229940023877 zeatin Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
CP0017-01-0123
ABSTRACT OF THE DISCLOSURE
The application belongs to the field of plant tissue culture, in particular to a
culture medium for promoting the elongation of clump buds of Vaccinium bracteatum
Thunb, a preparation method and an application thereof. The culture medium
comprises the following components: basic culture medium, plant growth regulator,
95 to 105g/L potato, 4.0 to 5.0g/L agar, 25 to 35g/L sucrose, pH is 5.8 to 6.0. In the
present invention, ZT is used as Plant Growth Regulator, which can transport nutrients
such as amino acids, auxins, and inorganic salts to the treatment site, which is
beneficial to stimulate the growth of buds; The potato contains starch, protein, fat,
crude fiber, various microorganisms and inorganic salts, etc., which not only provides
various nutrients required for the growth of clump buds, but also facilitates the further
elongation of the clump buds. Compared with the existing culture medium, the culture
medium of the invention can obviously induce the elongation of clump buds of
Vaccinium bracteatum Thunb, effectively shorten the seedling raising period, which is
of great significance for the intensive production of the tissue culture of the Vaccinium
bracteatum Thunb.
(FIG. 1)
CP0017-01-0123
FIG.1
FIG.2
1
Description
CP0017-01-0123
FIG.1
FIG.2
CP0017-01-0123
Culture medium for promoting the elongation of clump buds of Vaccinium bracteatum Thunb, a preparation method and an application thereof
[01] This application claims the priority of Chinese Patent Application No. 201911292473.2, entitled "Culture medium for promoting the elongation of clump buds of Vaccinium bracteatum Thunb, a preparation method and an application thereof' filed with the China National Intellectual Property Administration on December 12, 2019, which is incorporated herein by reference in its entirety. TECHNICAL FIELD
[02] The application belongs to the field of plant tissue culture, in particular to a culture medium for promoting the elongation of clump buds of Vaccinium bracteatum Thunb, a preparation method and an application thereof. BACKGROUNDART
[03] The Vaccinium bracteatum Thunb, also known as Nanzhu, belongs to evergreen shrubs or small trees of the Ericaceae(Vaccinium L.), mainly distributed in southern China. Its Chinese name obtained because people use its leaves to steam black rice on March 3 and April 8 of the lunar calendar every year. The berries and leaves of the Vaccinium bracteatum Thunb have high nutritional and medicinal value and thus of great development and utilization potential. Besides, the trunk is upright, the foliage is dense, the leave color is dense green, the fruit is gorgeous and beautiful, and the ornamental value is extremely high. It is a new star of landscape tree species. At present, the wild Vaccinium bracteatum Thunb is scattered in its distribution area, with a small number of individuals. In addition to human interference and bird or insect damages to the fruit, the number of distribution is decreasing day by day. It is likely to gradually lose in its community, if not expand protection, development and utilization. The existing technology has reported the method of seed germination of Vaccinium bracteatum Thunb, which makes it possible to sow and propagate. However, due to the fact that the fruits of wild Vaccinium bracteatum Thunb are less and difficult to pick, it is also difficult to achieve a large-scale development through sowing and propagation.
[04] Plant tissue culture is a kind of asexual propagation way developed in recent years according to the theory that plant cells have "totipotency". It plays an important role in rapid propagation of homogenized seedlings, virus-free seedling culture, genetic breeding, selection and utilization of mutants, cell fusion and DNA recombination. Plant tissue culture technology can effectively expand excellent varieties and provide a large number of high-quality seedlings in a short term, which makes the rapid development of the industry possible. However, the existing researches and practices show that the tissue culture technology of the Vaccinium bracteatum Thunb is not mature, since the existing media can not effectively induce the elongation of clump buds of Vaccinium bracteatum Thunb. SUMMARY OF THE INVENTION
[05] Therefore, the technical problem to be solved by the application is to overcome the defect that the existing culture medium can not effectively induce the elongation of clump buds of Vaccinium bracteatum Thunb, and therefore, the present invention provides a
CP0017-01-0123
culture medium for promoting the elongation of clump buds of Vaccinium bracteatum Thunb during tissue culture; at the same time, the invention also provides the preparation method and application of the culture medium.
[06] In order to solve the above technical problem, the invention provides a culture medium for promoting the elongation of clump buds of Vaccinium bracteatum Thunb, wherein the culture medium comprises the following components: basic culture medium, plant growth regulator, 95 to 105g/L potato, 4.0 to 5.0g/L agar, 25 to 35g/L sucrose, pH is 5.8 to 6.0.
[07] Further, the basic culture medium is 1.8 to 2.2g/L WPM culture medium, and the plant growth regulator is 0.8 to 1.2mg/L ZT.
[08] Further, the potato is a potato cube with side length of 0.2 to 1.0cm.
[09] Further, the cubes are irregular in shapes.
[10] Further, the culture medium comprises the following components: 2.0g/L WPM culture medium, 1.Omg/L ZT, 100g/L potato cubes, 4.5g/L agar, 30g/L sucrose; pH is 5.8 to 6.0.
[11] The invention also provides a preparation method of the culture medium for promoting the elongation of clump buds of the Vaccinium bracteatum Thunb, wherein including the following steps:
[12] (1) weighing the WPM culture medium powder, sucrose, agar, potato and a formulated amount of ZT aqueous solution;
[13] (2) adding the WPM culture medium powder, sucrose and ZT aqueous solution into water, mixing evenly, and adjusting the mixture to pH 5.8 to 6.0;
[14] (3) adding the agar, heating the mixture until the agar melts completely, then adding the potato, sealing and conducting sterilization to obtain the culture medium.
[15] Further, the step (1) further includes the step of cutting the potato into potato cubes with a side length of 0.2 to 1.0cm.
[16] Further, the sterilization in step (3) adopts steam sterilization with a pressure of 105kpa and a temperature of 121 °C for at least 15 minutes.
[17] The invention also provides the application of the culture medium for promoting the elongation of clump buds of Vaccinium bracteatum Thunb or the culture medium for promoting the elongation of clump buds of Vaccinium bracteatum Thunb prepared according to the preparation method, wherein the clump buds of the Vaccinium bracteatum Thunb are inoculated on the culture medium for cultivation.
[18] Furthermore, the culture conditions are: light intensity 1000 to 30001x, light time 12h/d, temperature 22 to 28 °C, duration 28 to 31 days.
[19] In the invention, ZT is the abbreviation of Zeatin, which is white crystal or powder.
[20] The technical scheme of the invention has the following advantages:
[21] 1. The invention provides a culture medium for promoting the elongation of clump buds of Vaccinium bracteatum Thunb, the culture medium includes basic culture medium, plant growth regulator, potato, agar and sucrose, the culture medium takes ZT as plant growth regulator, which can transfer amino acid, auxin, inorganic salt and other nutrients to the treatment site, which is facilitated to stimulate the growth of buds; the potato contains starch, protein, fat, crude fiber and various microorganisms and inorganic salts, etc., which not only provides various nutrients required by the growth of clump buds, but
CP0017-01-0123
also facilitates the further elongation of clump buds. Compared with the existing culture medium, the culture medium of the invention can obviously promote the elongation of the clump buds of Vaccinium bracteatum Thunb, it effectively shorten the seedling raising period, and it is of great significance for the intensive production of the tissue culture of the Vaccinium bracteatum Thunb.
[22] 2. The preparation method of the culture medium for promoting the elongation of clump buds of Vaccinium bracteatum Thunb provided by the invention simply involves mixing the components of the formula evenly and then autoclaving. The raw material is easy to obtain, the process is simple with strong operability, and it is convenient for large-scale promotion and application, which is of great significance for the improvement of tissue culture industrialization level of the Vaccinium bracteatum Thunb. BRIEFT DESCRIPTION OF THE DRAWINGS
[23] In order to more clearly explain the specific embodiments of the invention or the technical solutions in the prior art, the following will make a brief introduction to the drawings referred in description to the specific embodiments or the prior art. It is obvious that the drawings in the following description are some embodiments of the invention. Those ordinary skilled in the art can also obtain other drawings based on these drawings without creative labor.
[24] FIG.1 shows the picture taken after 30 days of cultivating of clump buds of Vaccinium bracteatum Thunb using the culture medium of Example 1 of the present invention;
[25] FIG.2 shows the picture taken after 30 days of cultivating of clump buds of Vaccinium bracteatum Thunb using the culture medium of comparative example 1 of the present invention;
[26] FIG.3 shows the picture taken after 30 days of cultivating of clump buds of Vaccinium bracteatum Thunb using the culture medium of comparative example 2 of the present invention. DETAILED DESCRIPTION OF THE EMBODIMENTS
[27] The following embodiments are provided for a better understanding of the present invention. The embodiments do not limit the content and protection scope of the invention. Any product which is the same or similar to the invention obtained by anyone under the enlightenment of the invention or by combining the features of the invention and other prior art falls within the protection scope of the invention.
[28] In the following embodiment, WPM culture medium powder is purchased from Hangzhou mumubio industry; The embodiment can be carried out according to the operation or conditions of the conventional experimental steps described in the literature in the art, if the specific experimental steps or conditions are not indicated. If the reagent or instrument used in the embodiment does not indicate the manufacturer, they are all conventional reagent products that can be obtained through market purchase.
[29] Example 1
[30] The embodiment provides a culture medium for promoting the elongation of clump buds of Vaccinium bracteatum Thunb, wherein the culture medium comprises the following components: 2.0 g/L WPM medium, 1.0 mg/L ZT, 100 g/L potato cubes, 4.5 g/L agar, 30 g/L sucrose; pH is 5.8 to 6.0. The preparation method comprised the
CP0017-01-0123
following steps:
[31] (1)The washed potatoes were cut into pieces, and 0.2 to 1.0cm potato cubes were obtained;
[32] (2) A proper amount of ZT powder was weighed out, added with a small amount of 1mol/L sodium hydroxide solution to dissolve, and then added with water to a constant volume to prepare 0.1 mg/L ZT aqueous solution;
[33] (3) 2.0 g WPM culture medium powder, 30 g sucrose, 4.5 g agar and 100 g potato cubes were weighed separately, and 10 ml ZT aqueous solution was measured;
[34] (4) the WPM culture medium powder, sucrose and ZT aqueous solution were added into water, mixed evenly, and the mixture was adjusted to pH 5.8 to 6.0;
[35] (5) After the agar was added, the mixture was heated until the agar was completely melted, sub packed into culture bottles, and then the potato cubes were added, the culture bottles were sealed and sterilized with high-pressure steam with a pressure of 105 kpa at a temperature of 121 °C for 20 minutes, so as to obtain the culture medium for promoting the elongation of clump buds of Vaccinium bracteatum Thunb.
[36] Example 2
[37] The embodiment provides a culture medium for promoting the elongation of clump buds of Vaccinium bracteatum Thunb, wherein the culture medium comprises the following components: 1.8 g/L WPM medium, 0.8 mg/L ZT, 105 g/L potato cubes, 4 g/L agar, 25 g/L sucrose; pH is 5.8 to 6.0. The preparation method comprised the following steps:
[38] (1)The washed potatoes were cut into pieces, and 0.2 to 1.0cm potato cubes were obtained;
[39] (2) A proper amount of ZT powder was weighed out, added with a small amount of 1mol/L sodium hydroxide solution to dissolve, and then added with water to a constant volume to prepare 0.1mg/L ZT aqueous solution;
[40] (3) 1.8 g WPM culture medium powder, 25 g sucrose, 4.Og agar and 105 g potato cubes were weighed separately, and 8 ml ZT aqueous solution was measured;
[41] (4) the WPM culture medium powder, sucrose and ZT aqueous solution were added into water, mixed evenly, and the mixture was adjusted to pH 5.8 to 6.0;
[42] (5) After the agar was added, the mixture was heated until the agar was completely melted, sub packed into culture bottles, and then the potato cubes were added, the culture bottles were sealed and sterilized with high-pressure steam with a pressure of 105kpa at a temperature of 121 °C for 15 minutes, so as to obtain the culture medium for promoting the elongation of clump buds of Vaccinium bracteatum Thunb.
[43] Example 3
[44] The embodiment provides a culture medium for promoting the elongation of clump buds of Vaccinium bracteatum Thunb, wherein the culture medium comprises the following components: 2.2 g/L WPM medium, 1.2 mg/L ZT, 95 g/L potato cubes, 4.5 g/L agar 35 g/L sucrose; pH is 5.8 to 6.0. The preparation method comprised the following steps:
[45] (1)The washed potatoes were cut into pieces, and 0.2 to 1.0cm potato cubes were obtained;
[46] (2) A proper amount of ZT powder was weighed out, added with a small amount of
CP0017-01-0123
1mol/L sodium hydroxide solution to dissolve, and then added with water to a constant volume to prepare 0.1 mg/L ZT aqueous solution;
[47] (3) 2.2 g WPM culture medium powder, 35 g sucrose, 4.5 g agar and 95 g potato cubes were weighed separately, and 12 ml ZT aqueous solution was measured;
[48] (4) the WPM culture medium powder, sucrose and ZT aqueous solution were added into water, mixed evenly, and the mixture was adjusted to pH5.8 to 6.0;
[49] (5) After the agar was added, the mixture was heated until the agar was completely melted, sub packed into culture bottles, and then the potato cubes were added, the culture bottles were sealed and sterilized with high-pressure steam with a pressure of 105 kpa at a temperature of 121 °C for 25 minutes, so as to obtain the culture medium for promoting the elongation of clump buds of Vaccinium bracteatum Thunb.
[50] comparative example 1
[51] This comparative example provides a culture medium, wherein the culture medium comprises the following components: 2.0 g/L WPM medium, 1.0 mg/L ZT, 100 g/L potato juice, 4.5 g/L agar, 30 g/L sucrose; pH is 5.8 to 6.0. The preparation method comprises the following steps:
[52] (1) Washed and peeled potatoes were cut into small pieces, 1OOg potato pieces were weighed, then it were boiled with water (boiled for 20-30 minutes, can be pierced by a glass rod), it were filtered with eight layers of gauze to obtain potato juice.
[53] (2) Appropriate amount of ZT powder was weighed out, added with a small amount of 1mol/L sodium hydroxide solution to dissolve, then water was added to a constant volume, and 0.1mg/L ZT aqueous solution was formulated;
[54] (3) 2.0 g WPM culture medium powder, 30 g sucrose, 4.5 g agar and 100 g potato juice were weighed separately, and 10 mL ZT aqueous solution was weighed;
[55] (4) the WPM culture medium powder, sucrose and ZT aqueous solution were added to the water, then were mixed evenly, and the mixture was adjusted to pH 5.8 to 6.0;
[56] (5) After the agar was added, the mixture was heated until the agar was completely melted, sub packed into culture bottles, and then the potato juice was added, the culture bottles were sealed and sterilized with high-pressure steam with a pressure of 105 kpa at a temperature of 121 °C for 20 minutes, so as to obtain the culture medium.
[57] comparative example 2
[58] This comparative example provides a culture medium, wherein the culture medium comprises the following components: 2.0 g/L WPM medium, 1.0 mg/L ZT, 4.5 g/L agar, g/L sucrose; pH is 5.8 to 6.0. The preparation method comprises the following steps:
[59] (1) Appropriate amount of ZT powder was weighed out, added with a small amount of 1mol/L sodium hydroxide solution to dissolve, then water was added to a constant volume, and 0.1mg/L ZT aqueous solution was formulated;
[60] (2) 2.0 g WPM culture medium powder, 30 g sucrose and 4.5 g agar were weighed separately, and 10 mL ZT aqueous solution were weighed;
[61] (3) WPM culture medium powder, sucrose and ZT aqueous solution were added to water, then were mixed evenly, and the mixture was adjusted to pH 5.8 to 6.0;
[62] (4) After the agar was added, the mixture was heated until the agar was completely melted, the mixture was sub packed into culture bottles, the culture bottles were sealed and sterilized with high-pressure steam with a pressure of 105 kpa at a temperature of 121
CP0017-01-0123
C for 20 minutes, so as to obtain the culture medium.
[631 comparative example 3
[64] This comparative example provides a culture medium, wherein the culture medium comprises the following components: 2.0 g/L WPM medium, 2.0 mg/L ZT, 4.5 g/L agar, g/L sucrose; pH is 5.8 to 6.0. The preparation method comprises the following steps:
[65] (1) Appropriate amount of ZT powder was weighed out, added with a small amount of 1mol/L sodium hydroxide solution to dissolve, then water was added to a constant volume, and 0.lmg/L ZT aqueous solution was formulated;
[661 (2) 2.0 g WPM culture medium powder, 30 g sucrose and 4.5 g agar were weighed separately, and 20 mL ZT aqueous solution was weighed;
[67] (3) the WPM culture medium powder, sucrose and ZT aqueous solution were added to water, then were mixed evenly, and the mixture was adjusted to pH 5.8 to 6.0;
[68] (4) After the agar was added, the mixture was heated until the agar was completely melted, the mixture was sub packed into culture bottles, the culture bottles were sealed and sterilized with high-pressure steam with a pressure of 105 kpa at a temperature of 121 C for 20 minutes, so as to obtain the culture medium.
[691 Experimental example
[701 The induced clump buds of Vaccinium bracteatum Thunb were transferred to the culture mediums of Example 1 and comparative examples 1 to 3 of the present invention respectively, and cultured for 30 days under the conditions of light intensity 20001x, light time 12h/d, and temperature 25 C. The growths of clump buds were shown in Table 1 below. The growths of the clump buds after 30 days of cultivation on the medium of Example 1 and comparative examples 1 and 2 were shown in FIGs.1 to 3.
Medium Growth of clump buds
The culture medium of A large number of clump buds have adventitious bud
Example 1 elongation, the average bud length is 2.02cm
The culture medium of A small number of clump buds have adventitious bud
comparative Example 1 elongation, the average bud length is 1.15cm
The culture medium of A small number of clump buds have adventitious bud
comparative Example 2 elongation, the average bud length is 1.02cm
The culture medium of Individual of clump buds have adventitious bud
comparative Example 3 elongation, the average bud length is 0.34cm
[711 According to table 1 and FIGs.1 to 3 above, the medium of the invention can obviously promote the elongation of clumped buds.
[721 Obviously, the above-mentioned embodiments are only examples for clear explanation, and they are not intended to limit the implementation. Those ordinary skilled in the art may make other changes or changes in different forms on the basis of the above description. There is no need and no way to exhaust all the ways of implementation. The obvious changes or changes thus induced are still within the protection scope of the
CP0017-01-0123
invention.
Claims (5)
1. A culture medium for promoting the elongation of clump buds of Vaccinium bracteatum Thunb, wherein the culture medium comprises the following components: basic culture medium, plant growth regulator, 95 to 105g/L potato, 4.0 to 5.0g/L agar, to 35g/L sucrose, pH is 5.8 to 6.0.
2. The culture medium according to claim 1, wherein the basic culture medium is 1.8-2.2g/L WPM culture medium, and the plant growth regulator is 0.8 to 1.2mg/l ZT.
3. The culture medium according to claim 1 or 2, wherein the potato is a potato cube with side length of 0.2 to 1.0cm.
4. A preparation method of the culture medium for promoting the elongation of clump buds of Vaccinium bracteatum Thunb according to any one of claims 1 to 3, wherein comprising the following steps:
(1) weighing the WPM culture medium powder, sucrose, agar, potato and a formulated amount of ZT aqueous solution;
(2) adding the WPM culture medium powder, sucrose and ZT aqueous solution into water, mixing evenly, and adjusting the mixture to pH 5.8 to 6.0;
(3) adding the agar, heating the mixture until the agar melts completely, then adding the potato, sealing and conducting sterilization to obtain the culture medium.
5. The preparation method according to claim 4, wherein the step (1) further includes the step of cutting the potato into potato cubes with a side length of 0.2 to 1.0cm.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911292473.2 | 2019-12-12 | ||
| CN201911292473.2A CN110810249B (en) | 2019-12-12 | 2019-12-12 | Culture medium for promoting elongation of oriental blueberry cluster buds as well as preparation method and application of culture medium |
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| AU2020101701A4 true AU2020101701A4 (en) | 2020-09-24 |
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| CN112470935A (en) * | 2020-12-18 | 2021-03-12 | 江苏省中国科学院植物研究所 | Oriental blueberry bud induction culture medium and tissue culture method |
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| CN106342688A (en) * | 2016-08-29 | 2017-01-25 | 盛林蓝莓集团股份有限公司 | Virus-free seedling method of blueberry germchit |
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| CN110810249B (en) | 2022-12-02 |
| CN110810249A (en) | 2020-02-21 |
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