CN105532121A - Sterile sowing method for ginseng seeds - Google Patents
Sterile sowing method for ginseng seeds Download PDFInfo
- Publication number
- CN105532121A CN105532121A CN201610010774.1A CN201610010774A CN105532121A CN 105532121 A CN105532121 A CN 105532121A CN 201610010774 A CN201610010774 A CN 201610010774A CN 105532121 A CN105532121 A CN 105532121A
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- CN
- China
- Prior art keywords
- ginseng seeds
- ginseng
- seeding method
- seeds
- aseptic
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
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- Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention provides a sterile sowing method for ginseng seeds, and belongs to the technical field of ginseng tissue culture. The method includes the steps of seed surface disinfection, inoculation pretreatment and inoculation, cold water immersion is used for simulating vernalization, ginseng seed dormancy is broken through ultrasonic treatment and KOH solution immersion treatment, and ginseng seeds are induced to germinate. The sterile sowing method for the ginseng seeds has the advantages of being low in cost and high in germination rate.
Description
Technical field
The invention belongs to ginseng tissue culture technique field, particularly a kind of ginseng seeds aseptic seeding method.
Background technology
Ginseng is Angiospermae Dicotyledoneae Archichlamydeae Araliaceae, herbaceos perennial, be grown on thick forest, be distributed in Heilungkiang, Jilin, Liaoning and northern Hebei remote mountains more, containing more than 10 kinds of panaxosides, and the fast alcohol of ginseng, several amino acids and vitamin, be famous and precious Chinese medicinal herbs.
But the material of a large amount of Inhibiting germination after ginseng seeds maturation, can be accumulated, stop ginseng seeds to be sprouted, make the dormancy of ginseng seeds penetration depth, difficulty is sprouted in normal sterile type of seeding, and germination rate is low.
Summary of the invention
There are the problems referred to above based on prior art, the invention provides a kind of ginseng seeds aseptic seeding method, the method comprises the surface of the seed sterilization, step such as inoculation pre-treatment and inoculation etc., cold water soak is utilized to simulate vernalization, again by ultrasonic process and KOH solution immersion treatment, break ginseng seeds dormancy, induction ginseng seeds is sprouted, and ginseng seeds aseptic seeding method provided by the invention has the advantage that cost is low, germination rate is high.
A kind of ginseng seeds aseptic seeding method, its special war is, it comprises the following steps:
Step S10: the ripe ginseng seeds getting full grains, carries out cold water soak process to seed, then in gnotobasis, carries out surface sterilization to ginseng seeds;
Step S20: ultrasonic process and KOH solution immersion treatment are carried out to the complete ginseng seeds of sterilization;
Step S30: the ginseng seeds be disposed by step S20 is inoculated into ginseng seeds inducing culture, and put into climatic cabinate and cultivate.
A kind of ginseng seeds aseptic seeding method according to claim 1, it is characterized in that, step S10 comprises S11, uses the cold water of 8-12 DEG C to carry out immersion 18-24 hour to ginseng seeds, use cold water soak can simulating nature vernalization, break ginseng seeds dormancy.
A kind of ginseng seeds aseptic seeding method according to claim 1, it is characterized in that, step S10 comprises S12 surface sterilization, condition is alcohol-pickled 25-30 second of 70-75% by mass concentration, with aseptic water washing 3-5 time, be the mercuric chloride solution sterilization 6-8 minute of 0.10-0.12% again by mass concentration, then use aseptic water washing 3-5 time.
A kind of ginseng seeds aseptic seeding method according to claim 1, it is characterized in that, step S20 comprises step S21, working concentration is the KOH solution immersion treatment 8-10 minute of 0.05-0.1mol/L, rinse 3-5 time by sterile water wash, use the KOH of low concentration can corrode seed seed coat, discharge Inhibiting germination in seed material.
A kind of ginseng seeds aseptic seeding method according to claim 1, it is characterized in that, step S20 comprises step S22, the ultrasonic that frequency of utilization is 50-60KHz, power is 80-90W is to ginseng seeds process 25-30 minute, super refreshing ripple process can regulate the physiological status of ginseng seeds, promotes seed germination.
A kind of ginseng seeds aseptic seeding method according to claim 1, it is characterized in that, medium in step S30 is medium based on 1/2MS medium, and adding the methyl α-naphthyl acetate of 1.0-1.2mg/L, the 6-benzyl aminoadenine of 1.5-2.0mg/L, the agar powder of 8-12g/L and the white granulated sugar of 28-32g/L, high-temperature sterilization is made.
A kind of ginseng seeds aseptic seeding method according to claim 1, it is characterized in that, the condition of culture in step S30 is: light dark period is that 14h illumination/12h is dark, intensity of illumination is be cultured under the condition of 2000-2300lux to grow up to seedling.
Embodiment
Below in conjunction with specific embodiment, the invention will be further described.
The methyl α-naphthyl acetate of each composition in 1/2MS medium and 1.0mg, the 6-benzyl aminoadenine of 1.5mg, the agar powder of 10 and the white granulated sugar of 30g is taken respectively according to the 1/2MS culture medium prescription shown in table 1 with balance, be placed in triangular flask, add appropriate purified water, stirring and dissolving, be settled to 1000mL, and adjust ph to 6.2.Triangular flask is sealed, is placed in high-pressure sterilizing pot, in 121 DEG C of sterilizing 21min, then in superclean bench, medium is divided and is filled in tissue culture flasks, naturally cool, by tissue cultures bottle closure after it solidifies, for subsequent use.
The composition of table 11/2MS medium and consumption thereof.
Get the ripe ginseng seeds of full grains, the cold water of 10 DEG C is used to carry out immersion 20 hours to ginseng seeds, take out seed, and be alcohol-pickled 30 seconds of 75% by seed mass concentration in superclean bench, with aseptic water washing 4 times, sterilize 6 minutes with the mercuric chloride solution that mass concentration is 0.12% again, then use aseptic water washing 4 times.
It is the KOH solution immersion treatment 9 minutes of 0.07mol/L by the above-mentioned ginseng working concentration cleaned up, 5 times are rinsed again by sterile water wash, take out that seed frequency of utilization are 55KHz, power be the ultrasonic of 85W to ginseng seeds process 30 minutes, take out seed, for subsequent use.
The ginseng seeds be disposed is inoculated into the above-mentioned ginseng seeds inducing culture prepared, and put into climatic cabinate and cultivate, condition of culture is light dark period is that 14h illumination/12h is dark, intensity of illumination is cultivate under the condition of 2000-2300lux, cultivate to starting there be first seed germination when the 15th day, grow up to ginseng aseptic seedling, cultivate after 22 days, ginseng seeds more than 90% is sprouted, induce into aseptic seedling, seed germination rate is up to 90%, higher than the germination rate of conventional abductive approach, the average sprout time of conventional abductive approach is after 25 days, aseptic seeding method provided by the invention is used to shorten induction time.Reagent in addition selected by aseptic seeding method provided by the invention and equipment such as KOH solution and ultrasonic equipment etc. all belong to Routine Test Lab equipment, easy to use and cost is low, greatly can reduce derived cost.
Claims (7)
1. a ginseng seeds aseptic seeding method, its special war is, it comprises the following steps:
Step S10: the ripe ginseng seeds getting full grains, carries out cold water soak process to seed, then in gnotobasis, carries out surface sterilization to ginseng seeds;
Step S20: ultrasonic process and KOH solution immersion treatment are carried out to the complete ginseng seeds of sterilization;
Step S30: the ginseng seeds be disposed by step S20 is inoculated into ginseng seeds inducing culture, and put into climatic cabinate and cultivate.
2. a kind of ginseng seeds aseptic seeding method according to claim 1, it is characterized in that, step S10 comprises S11,
The cold water of 8-12 DEG C is used to carry out immersion 18-24 hour to ginseng seeds.
3. a kind of ginseng seeds aseptic seeding method according to claim 1, it is characterized in that, step S10 comprises S12 surface sterilization, condition is alcohol-pickled 25-30 second of 70-75% by mass concentration, with aseptic water washing 3-5 time, be the mercuric chloride solution sterilization 6-8 minute of 0.10-0.12% again by mass concentration, then use aseptic water washing 3-5 time.
4. a kind of ginseng seeds aseptic seeding method according to claim 1, it is characterized in that, step S20 comprises step S21, and working concentration is the KOH solution immersion treatment 8-10 minute of 0.05-0.1mol/L, rinses 3-5 time by sterile water wash.
5. a kind of ginseng seeds aseptic seeding method according to claim 1, it is characterized in that, step S20 comprises step S22, and the ultrasonic that frequency of utilization is 50-60KHz, power is 80-90W is to ginseng seeds process 25-30 minute.
6. a kind of ginseng seeds aseptic seeding method according to claim 1, it is characterized in that, medium in step S30 is medium based on 1/2MS medium, and adding the methyl α-naphthyl acetate of 1.0-1.2mg/L, the 6-benzyl aminoadenine of 1.5-2.0mg/L, the agar powder of 8-12g/L and the white granulated sugar of 28-32g/L, high-temperature sterilization is made.
7. a kind of ginseng seeds aseptic seeding method according to claim 1, it is characterized in that, the condition of culture in step S30 is: light dark period is that 14h illumination/12h is dark, intensity of illumination is be cultured under the condition of 2000-2300lux to grow up to seedling.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201610010774.1A CN105532121A (en) | 2016-01-09 | 2016-01-09 | Sterile sowing method for ginseng seeds |
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| CN201610010774.1A CN105532121A (en) | 2016-01-09 | 2016-01-09 | Sterile sowing method for ginseng seeds |
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| CN201610010774.1A Pending CN105532121A (en) | 2016-01-09 | 2016-01-09 | Sterile sowing method for ginseng seeds |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106962375A (en) * | 2017-03-27 | 2017-07-21 | 贵州大学 | Radix pseudostellariae kind root inorganic agent and its application |
| CN108271458A (en) * | 2017-12-28 | 2018-07-13 | 江苏哈工药机科技股份有限公司 | A kind of ginseng seeds Sterile culture method |
| CN109105233A (en) * | 2018-08-08 | 2019-01-01 | 山东省果树研究所 | The copper coin tree seedling fostering method and culture medium quickly bred based on micro cuttage |
| CN111587773A (en) * | 2020-05-08 | 2020-08-28 | 内蒙古自治区生物技术研究院 | Rapid soilless green seedling culture method for astragalus membranaceus |
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| CN101156553A (en) * | 2007-11-16 | 2008-04-09 | 中国科学院新疆理化技术研究所 | a method for rapid evoking adventive bud panax ginseng stem segment |
| CN102487626A (en) * | 2011-12-08 | 2012-06-13 | 吉林省集安益盛药业股份有限公司 | Physical treatment method for ginseng seeds |
| CN104798492A (en) * | 2015-03-30 | 2015-07-29 | 嘉兴职业技术学院 | Experimental method for increasing germination rate of Talinum paniculatum |
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2016
- 2016-01-09 CN CN201610010774.1A patent/CN105532121A/en active Pending
Patent Citations (3)
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| CN101156553A (en) * | 2007-11-16 | 2008-04-09 | 中国科学院新疆理化技术研究所 | a method for rapid evoking adventive bud panax ginseng stem segment |
| CN102487626A (en) * | 2011-12-08 | 2012-06-13 | 吉林省集安益盛药业股份有限公司 | Physical treatment method for ginseng seeds |
| CN104798492A (en) * | 2015-03-30 | 2015-07-29 | 嘉兴职业技术学院 | Experimental method for increasing germination rate of Talinum paniculatum |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106962375A (en) * | 2017-03-27 | 2017-07-21 | 贵州大学 | Radix pseudostellariae kind root inorganic agent and its application |
| CN106962375B (en) * | 2017-03-27 | 2019-11-08 | 贵州大学 | Radix pseudostellariae kind root inorganic agent and its application |
| CN108271458A (en) * | 2017-12-28 | 2018-07-13 | 江苏哈工药机科技股份有限公司 | A kind of ginseng seeds Sterile culture method |
| CN108271458B (en) * | 2017-12-28 | 2021-06-22 | 江苏哈工药机科技股份有限公司 | Ginseng seed sterile culture method |
| CN109105233A (en) * | 2018-08-08 | 2019-01-01 | 山东省果树研究所 | The copper coin tree seedling fostering method and culture medium quickly bred based on micro cuttage |
| CN111587773A (en) * | 2020-05-08 | 2020-08-28 | 内蒙古自治区生物技术研究院 | Rapid soilless green seedling culture method for astragalus membranaceus |
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Application publication date: 20160504 |