CN108271458B - Ginseng seed sterile culture method - Google Patents
Ginseng seed sterile culture method Download PDFInfo
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- CN108271458B CN108271458B CN201711451992.XA CN201711451992A CN108271458B CN 108271458 B CN108271458 B CN 108271458B CN 201711451992 A CN201711451992 A CN 201711451992A CN 108271458 B CN108271458 B CN 108271458B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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Abstract
The invention discloses a ginseng seed sterile culture method, wherein a culture system adopted by the culture method comprises a sterile water storage tank, a nutrient solution preparation tank, a transfer tank, a culture tank, a steam supply system, a sterile oxygen supply system and a pure water supply system; and (3) sending the pure water in the sterile water storage tank into a nutrient solution preparation tank, adding the nutrient solution, stirring to prepare nutrient solution, then sending the nutrient solution into a transfer tank, adding ginseng seeds for pre-culture, and then sending into a culture tank for culture. The ginseng seed sterile culture method can perform germination accelerating treatment on ginseng seeds in a sterile environment, improves the germination rate of the ginseng seeds, and can shorten the germination accelerating period.
Description
Technical Field
The invention relates to a culture method, in particular to a ginseng seed sterile culture method.
Background
Ginseng is a common herb used for recovery after healing, enhancing physical strength, regulating hormones, lowering blood sugar and blood pressure, controlling liver index and liver function health care, etc. However, the difficulty of the current artificial planting of ginseng lies in the germination problem of ginseng seeds, because ginseng is mainly propagated by seeds, the current operation mode is mainly that ginseng seeds are put on the surface of soil added with nutrient components, then put into a germination accelerating box and put in a proper place indoors or outdoors for accelerating germination, the germination period is long, the ginseng seeds can germinate generally after 8-9 months of germination accelerating treatment, and the germination rate is not high due to the limitation of the temperature, humidity and nutrient of the environment.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: the ginseng seed sterile culture method can accelerate germination of the ginseng seeds in a sterile environment, improve the germination rate of the ginseng seeds and shorten the germination accelerating period.
In order to solve the technical problems, the technical scheme of the invention is as follows: a ginseng seed sterile culture method comprises the following steps:
s1, carrying out high-temperature sterilization treatment on the interiors of a sterile water storage tank, a nutrient solution preparation tank, a transfer tank, a culture tank and other pipelines of the culture system; the culture system comprises an aseptic water storage tank, a nutrient solution preparation tank, a transfer tank, a culture tank, a steam supply system, an aseptic oxygen supply system and a pure water supply system;
the water inlet of the sterile water storage tank is communicated with a pure water supply system, the sterile water storage tank is provided with a steam sterilization inlet and a pressurization port, the steam sterilization inlet is communicated with the steam supply system, the pressurization port is communicated with a sterile oxygen supply system, the bottom of the sterile water storage tank is provided with a discharge port, and the discharge port is communicated with a discharge main pipeline;
the nutrient solution preparation tank is provided with an outer jacket and a stirring device, a water inlet of the nutrient solution preparation tank is communicated with a discharge port of the sterile water storage tank through a sterile water supply pipeline, the top of the nutrient solution preparation tank is provided with a steam sterilization inlet, the outer jacket of the nutrient solution preparation tank is provided with a steam heating inlet, a steam heating outlet, a cooling water inlet and a cooling water outlet, the steam heating inlet and the steam sterilization inlet are communicated with a steam supply system, the cooling water inlet and the cooling water outlet are communicated with the cooling water supply system, the bottom of the nutrient solution preparation tank is provided with a discharge port, the discharge port is connected with one end of a nutrient solution transfer main pipeline, and the discharge port is also communicated with a discharge main pipeline;
the device comprises a transfer tank, a feed port of the transfer tank, a discharge port of a nutrient solution preparation tank, a nutrient solution transfer main pipeline, a steam sterilization inlet of the transfer tank, an aeration system, a sterile oxygen supply system pipeline, a discharge port, a discharge main pipeline, a constant temperature control system and a constant temperature control system, wherein the transfer tank is provided with an outer jacket;
the culture tank is provided with an outer jacket, a feed inlet on the culture tank is communicated with the other end of the discharge transfer pipeline, a liquid inlet on the culture tank is communicated with the nutrient solution transfer main pipeline, a steam sterilization inlet of the culture tank is communicated with the steam supply system, a discharge outlet at the bottom of the culture tank is provided with an aeration system, the aeration system is communicated with the sterile oxygen supply system pipeline, a discharge outlet of the culture tank is communicated with the discharge main pipeline, and the outer jacket of the culture tank is communicated with the constant temperature control system;
s2, adding sterile water into the nutrient solution preparation tank from the water storage tank, and adding the culture solution into the nutrient solution preparation tank to be stirred and mixed to form nutrient solution;
s3, heating and sterilizing the nutrient solution in the nutrient solution preparation tank for 1-1.5h at the temperature of 100-120 ℃;
s4, cooling the nutrient solution preparation tank to reduce the temperature of the nutrient solution to 30-40 ℃;
s5, conveying the nutrient solution from the nutrient solution preparation tank to a transfer tank;
s6, putting the ginseng seeds into a transfer tank under the aseptic operation environment, culturing for 5-10 days at the temperature of 27.5-28.5 ℃, and continuously introducing oxygen for aeration at the aeration pressure of 0.15-0.35Mpa during the culture period;
s7, transferring the culture solution and the ginseng seeds in the transfer tank to a culture tank for culture, culturing for 65-80 days at the temperature of 27.5-28.5 ℃, and continuously introducing oxygen into the transfer tank for aeration at the aeration pressure of 0.15-0.35 Mpa;
s8, discharging the culture solution in the culture tank, and taking out the germinated ginseng seedlings.
In the preferred scheme, the high-temperature sterilization methods of the interiors of the sterile water storage tank, the transfer tank and the culture tank are all steam sterilization, steam is introduced from the tops of the sterile water storage tank, the transfer tank and the culture tank, and is discharged from the bottoms of the transfer tank and the culture tank for sterilization; the sterilization method of the nutrient solution preparation tank comprises the following steps: introducing steam into an outer jacket of the nutrient solution preparation tank for heating, introducing sterile oxygen into the bottom of the nutrient solution preparation tank and discharging from the top, then closing the top for exhausting, introducing steam from the top and discharging gas in the nutrient solution preparation tank from the bottom in a replacement manner.
In a preferred scheme, the nutrient solution in the nutrient solution preparation tank is heated and sterilized in the following mode: introducing steam into an outer jacket of the nutrient solution preparation tank, introducing the steam into the nutrient solution preparation tank when the temperature in the nutrient solution preparation tank reaches 100 ℃, stopping introducing the steam and keeping the steam when the temperature in the tank reaches 120 ℃, introducing the steam when the temperature in the tank reaches 100 ℃, and circulating for 1.5 hours at the temperature of 100-120-100 ℃.
Preferably, in step S6, the ginseng seeds are placed in the transfer pot under sterile operation environment in a manner that: utilize a workstation cover of taking the operation hole to detain in the upper end of shifting the jar, the upper end of shifting the jar is provided with puts into the mouth, sets up in the workstation and utilizes the ultraviolet lamp sterilization more than 12 hours, then the alcohol disinfection of operator's both hands, to the outer mouthful disinfection of the household utensils of splendid attire ginseng seed, the operator puts into the household utensils of splendid attire ginseng seed from the operation hole and carries out ultraviolet sterilization minimum 20min, then pours the ginseng seed into in the shifting jar.
After the technical scheme is adopted, the invention has the effects that: the culture method utilizes the culture system, can culture ginseng seeds in a sterile environment, utilizes the nutrient solution preparation tank to prepare nutrient solution, carries out deep disinfection, then utilizes the transfer tank to culture the ginseng seeds for one week, transfers the ginseng seeds into the culture tank to culture, can reduce the volume of the transfer tank, simultaneously carries out certain stirring effect after being cultured in the transfer tank in advance and then being sent into the culture tank, has the total culture period of 80 days and short culture period,
the high-temperature sterilization methods of the interiors of the sterile water storage tank, the transfer tank and the culture tank are steam sterilization, and steam is introduced from the tops of the sterile water storage tank, the transfer tank and the culture tank and is discharged from the bottoms of the transfer tank and the culture tank for sterilization; the sterilization method of the nutrient solution preparation tank comprises the following steps: the steam heating is let in at the outer jacket of nutrient solution preparation jar earlier, aseptic oxygen is let in and is discharged from the top to the bottom of nutrient solution preparation jar simultaneously, then close the top exhaust, let in steam from the top and prepare jar interior gas replacement with the nutrient solution and discharge from the bottom, this kind of sterilization mode convenient operation, and to the nutrient solution preparation jar because inside agitating unit also, consequently, adopt two steps sterilization mode during the sterilization, at first outer gum cover sterilization, inside carries out gas replacement simultaneously, discharge the bacterium, then carry out the inside sterilization of steam and discharge from the bottom again, so not only can realize quick sterilization and the sterilization effect is more thorough.
The mode of heating and sterilizing the nutrient solution in the nutrient solution preparation tank is as follows: introducing steam into an outer jacket of the nutrient solution preparation tank, introducing the steam into the nutrient solution preparation tank when the temperature in the nutrient solution preparation tank reaches 100 ℃, stopping introducing the steam and keeping the steam when the temperature in the tank reaches 120 ℃, introducing the steam when the temperature in the tank reaches 100 ℃, and circulating for 1.5h at the temperature of 100-120-100 ℃ in the way, wherein the heating sterilization mode can improve the sterilization effect.
Drawings
The invention is further illustrated with reference to the following figures and examples.
FIG. 1 is a first partial schematic diagram of an embodiment of the present invention;
FIG. 2 is a second partial schematic structural diagram of an embodiment of the present invention;
FIG. 3 is a third schematic view of a partial structure according to an embodiment of the present invention;
FIG. 4 is a partial block diagram of an embodiment of the present invention;
FIG. 5 is a fifth schematic structural view of a culture tank according to an embodiment of the present invention;
in the drawings: 1. a sterile water storage tank; 101. a steam sterilization inlet; 102. a pressurizing port; 103. an outlet port; 2. a steam supply system; 3. a sterile oxygen supply system; 4. a pure water supply system; 5. a discharge header pipe; 6. a sterile water supply conduit; 7. a nutrient solution preparation tank; 71. a water inlet; 72. a steam sterilization inlet; 73. a steam heating inlet; 74. a steam heating outlet; 75. a cooling water inlet; 76. a cooling water outlet; 77. a discharge port; 78. a stirring device; 8. a nutrient solution transfer main pipeline; 9. a transfer tank; 91. a steam sterilization inlet; 92. a feed inlet; 93 a discharge hole; 94. an aeration system; 941. a connecting seat; 942. a connecting flange; 943. an aeration plate; 944. aerating the ball body; 95. a constant temperature control system; 951. a water inlet pipe; 952. a water outlet pipe; 953. a discharge pipe; 954. a recycling pipe; 955. an electric heater; 10. a discharge transfer pipe; 11. a culture tank; 111. a feed inlet; 112. a liquid inlet; 113. a steam sterilization inlet; 114. and (5) spraying the ball.
Detailed Description
The present invention is described in further detail below with reference to specific examples.
As shown in fig. 1 to 5, fig. 1 to 5 disclose a cultivation system, in order to better embody the connection relationship in the system, the cultivation system is disassembled and cut, as shown in fig. 1 to 5, the same letter in the adjacent position is a pipe, taking fig. 1 and 2 as an example, and a position a in fig. 1 and 2 is a pipe; a pipeline is arranged at the position B in the figure 1 and the position B in the figure 2.
A ginseng seed sterile culture system comprises a sterile water storage tank 1, a nutrient solution preparation tank 7, a transfer tank 9, a culture tank 11, a steam supply system 2, a sterile oxygen supply system 3 and a pure water supply system 4; the pure water supply system 4 supplies sterile pure water by adopting a water purifier; the steam supply system adopts steam provided by a boiler; the sterile oxygen supply system 3 uses an air compressor to provide oxygen.
The water inlet of the sterile water storage tank 1 is communicated with a pure water supply system 4, the sterile water storage tank 1 is provided with a steam sterilization inlet 101 and a pressurizing port 102, the steam sterilization inlet 101 is communicated with a steam supply system 2, the pressurizing port 102 is communicated with a sterile oxygen supply system 3, and the pressurizing port 102 is mainly used for pressurizing the upper part of the liquid level of the sterile water storage tank 1 to force pure water in the sterile water storage tank 1 to enter a nutrient solution preparation tank 7.
The bottom of the sterile water storage tank 1 is provided with a discharge port 103, and the discharge port 103 is communicated with a discharge manifold 5.
The nutrient solution preparation tank 7 is a nutrient solution preparation tank 7 with an outer jacket and a stirring device 78, and the stirring device 78 adopts a motor and a stirring blade to stir and mix.
The water inlet 71 of the nutrient solution preparation tank 7 is communicated with the discharge port 103 of the sterile water storage tank 1 through a sterile water supply pipeline 6, the top of the nutrient solution preparation tank 7 is provided with a steam sterilization inlet 72, and two steam sterilization inlets 72 and the end parts of the nutrient solution preparation tank 7 are provided with spraying balls 114.
The outer jacket of the nutrient solution preparation tank 7 is provided with a steam heating inlet 73, a steam heating outlet 74, a cooling water inlet 75 and a cooling water outlet 76, the steam heating inlet 73 and the steam sterilization inlet 72 are both communicated with the steam supply system 2, the cooling water inlet 75 and the cooling water outlet 76 are communicated with the cooling water supply system, the cooling water supply system can be cooled by tap water, the bottom of the nutrient solution preparation tank is provided with a discharge hole 77, the discharge hole 77 is connected with one end of a nutrient solution transfer main pipeline 8, and the discharge hole 77 is also communicated with a discharge main pipeline 5.
The transfer tank 9 is a transfer tank 9 with an outer jacket, a feed inlet 92 of the transfer tank 9 is communicated with a discharge outlet 77 of a nutrient solution preparation tank 7 and a nutrient solution transfer main pipeline 8, a steam sterilization inlet 91 of the transfer tank 9 is communicated with a steam supply system 2, a discharge outlet 93 at the bottom of the transfer tank 9 is provided with an aeration system 94, the aeration system 94 is communicated with a sterile oxygen supply system 3 pipeline, the discharge outlet 93 is communicated with one end of a discharge transfer pipeline 10, the discharge outlet 93 is also communicated with a discharge main pipeline 5, and an outer jacket of the transfer tank 9 is provided with a constant temperature control system 95 communicated with the transfer tank 9 for heat preservation;
the culture tank 11 is provided with an outer jacket, a feed inlet 111 on the culture tank 11 is communicated with the other end of the discharge transfer pipeline 10, a liquid inlet 112 on the culture tank 11 is communicated with the nutrient solution transfer main pipeline 8, a steam sterilization inlet 113 of the culture tank 11 is communicated with the steam supply system 2, a bottom discharge port 93 of the culture tank 11 is provided with an aeration system 94, the aeration system 94 is communicated with the sterile oxygen supply system 3 through a pipeline, a discharge port of the culture tank 11 is communicated with the discharge main pipeline 5, and the outer jacket of the culture tank 11 is communicated with the constant temperature control system 95. A self-circulation pipeline 116 is connected between the outlet of the culture tank 11 and the steam sterilization inlet 113, a self-circulation pump is arranged on the self-circulation pipeline, and a spraying ball 114 is arranged at the end part of the steam sterilization inlet 113 on the culture tank 11. The side of the culture tank 11 is provided with a blowing inlet which is communicated with the sterile oxygen supply system 3 through a blowing pipeline. In this embodiment, the number of culture tanks 11 is plural and the culture tanks are arranged in parallel.
The aeration systems 94 structure of culture tank 11 and transfer jar 9 bottom is the same, and this aeration systems 94 includes connecting seat 941, and connecting seat 941 can dismantle with the discharge gate of culture tank 11 or the flange 942 on the discharge gate 93 of transfer jar 9 and be connected, be provided with aeration plate 943 on the connecting seat 941, be provided with a plurality of aeration spheroid 944 on the aeration plate, set up the aeration micropore on the aeration spheroid 944, aeration spheroid 944 passes through aeration plate 943 and aseptic oxygen supply system 3 intercommunication. The constant temperature control system 95 on the transfer tank 9 comprises an inlet pipe 951 and an outlet pipe 952 which are arranged on an outer jacket of the transfer tank 9, a water pump is arranged on the inlet pipe, a cooling water supply system is arranged on the inlet pipe, the outlet pipe is connected with a discharge pipe 953, a circulation recycling pipe 954 is arranged between the outlet pipe and the inlet pipe, and an electric heater 955 is arranged on the circulation recycling pipe.
The embodiment also discloses a ginseng seed sterile culture method, which uses the culture system and comprises the following steps:
s1, performing high-temperature sterilization treatment on the interiors of the sterile water storage tank 1, the nutrient solution preparation tank 7, the transfer tank 9, the culture tank 11 and other pipelines of the culture system; in the preferred scheme, the high-temperature sterilization methods in the sterile water storage tank 1, the transfer tank 9 and the culture tank 11 are steam sterilization, steam is introduced from the tops of the sterile water storage tank 1, the transfer tank 9 and the culture tank 11, and is discharged from the bottoms of the transfer tank 9 and the culture tank 11 for sterilization; the sterilization method of the nutrient solution preparation tank 7 comprises the following steps: introducing steam into an outer jacket of the nutrient solution preparation tank for heating, introducing sterile oxygen into the bottom of the nutrient solution preparation tank and discharging from the top, then closing the top for exhausting, introducing steam from the top and discharging gas in the nutrient solution preparation tank from the bottom in a replacement manner.
S2, adding sterile water into the nutrient solution preparation tank 7 from the water storage tank, and adding the culture solution into the nutrient solution preparation tank to be stirred and mixed to form nutrient solution; the nutrient solution preparation tank is provided with a nutrient solution adding port.
S3, heating and sterilizing the nutrient solution in the nutrient solution preparation tank 7 for 1-1.5h at the temperature of 100-; the heating sterilization mode of the nutrient solution in the nutrient solution preparation tank 7 is as follows: introducing steam into an outer jacket of the nutrient solution preparation tank 7, introducing the steam into the nutrient solution preparation tank 7 when the temperature in the nutrient solution preparation tank reaches 100 ℃, stopping introducing the steam and keeping the steam when the temperature in the tank reaches 120 ℃, introducing the steam when the temperature in the tank reaches 100 ℃, and circulating for 1.5 hours at the temperature of 100-120-100 ℃.
S4, cooling the nutrient solution preparation tank 7 to reduce the temperature of the nutrient solution to 30-40 ℃;
s5, conveying the nutrient solution from the nutrient solution preparation tank to the transfer tank 9;
s6, putting the ginseng seeds into a transfer tank 9 under the aseptic operation environment, culturing for 5-10 days at the temperature of 27.5-28.5 ℃, and continuously introducing oxygen for aeration at the aeration pressure of 0.15-0.35Mpa during the culture period; in the step S6, the ginseng seeds are put into the transfer pot 9 in a sterile operating environment in such a manner that: utilize a workstation cover of taking the operation hole to detain in the upper end of shifting jar 9, the upper end of shifting jar 9 is provided with puts into the mouth, sets up in the workstation and utilizes ultraviolet lamp sterilization more than 12 hours, then the alcohol disinfection of operator's both hands, to the outer mouthful disinfection of the household utensils of splendid attire ginseng seed, the operator puts into the workstation with the household utensils of splendid attire ginseng seed from the operation hole and carries out ultraviolet sterilization minimum 20min, then pours the ginseng seed into in shifting jar 9.
S7, transferring the culture solution in the transfer tank 9 and the ginseng seeds into a culture tank 11 for culture, culturing at the temperature of 27.5-28.5 ℃ for 65-80 days, and continuously introducing oxygen into the transfer tank 9 for aeration at the aeration pressure of 0.15-0.35 Mpa;
s8, discharging the culture solution in the culture tank 11, and taking out the germinated Ginseng radix seedling.
The above-mentioned embodiments are merely descriptions of the preferred embodiments of the present invention, and are not intended to limit the scope of the present invention, and various modifications and alterations made to the technical solution of the present invention without departing from the spirit of the present invention are intended to fall within the scope of the present invention defined by the claims.
Claims (4)
1. A ginseng seed sterile culture method is characterized by comprising the following steps: the culture method comprises the following steps:
s1, carrying out high-temperature sterilization treatment on the interiors of a sterile water storage tank, a nutrient solution preparation tank, a transfer tank, a culture tank and other pipelines of the culture system; the culture system comprises an aseptic water storage tank, a nutrient solution preparation tank, a transfer tank, a culture tank, a steam supply system, an aseptic oxygen supply system and a pure water supply system;
the water inlet of the sterile water storage tank is communicated with a pure water supply system, the sterile water storage tank is provided with a steam sterilization inlet and a pressurization port, the steam sterilization inlet is communicated with the steam supply system, the pressurization port is communicated with a sterile oxygen supply system, the bottom of the sterile water storage tank is provided with a discharge port, and the discharge port is communicated with a discharge main pipeline;
the nutrient solution preparation tank is provided with an outer jacket and a stirring device, a water inlet of the nutrient solution preparation tank is communicated with a discharge port of the sterile water storage tank through a sterile water supply pipeline, the top of the nutrient solution preparation tank is provided with a steam sterilization inlet, the outer jacket of the nutrient solution preparation tank is provided with a steam heating inlet, a steam heating outlet, a cooling water inlet and a cooling water outlet, the steam heating inlet and the steam sterilization inlet are communicated with a steam supply system, the cooling water inlet and the cooling water outlet are communicated with the cooling water supply system, the bottom of the nutrient solution preparation tank is provided with a discharge port, the discharge port is connected with one end of a nutrient solution transfer main pipeline, and the discharge port is also communicated with a discharge main pipeline;
the device comprises a transfer tank, a feed port of the transfer tank, a discharge port of a nutrient solution preparation tank, a nutrient solution transfer main pipeline, a steam sterilization inlet of the transfer tank, an aeration system, a sterile oxygen supply system pipeline, a discharge port, a discharge main pipeline, a constant temperature control system and a constant temperature control system, wherein the transfer tank is provided with an outer jacket;
the culture tank is provided with an outer jacket, a feed inlet on the culture tank is communicated with the other end of the discharge transfer pipeline, a liquid inlet on the culture tank is communicated with the nutrient solution transfer main pipeline, a steam sterilization inlet of the culture tank is communicated with the steam supply system, a discharge outlet at the bottom of the culture tank is provided with an aeration system, the aeration system is communicated with the sterile oxygen supply system pipeline, a discharge outlet of the culture tank is communicated with the discharge main pipeline, and the outer jacket of the culture tank is communicated with the constant temperature control system;
s2, adding sterile water into the nutrient solution preparation tank from the water storage tank, and adding the culture solution into the nutrient solution preparation tank to be stirred and mixed to form nutrient solution;
s3, heating and sterilizing the nutrient solution in the nutrient solution preparation tank for 1-1.5h at the temperature of 100-120 ℃;
s4, cooling the nutrient solution preparation tank to reduce the temperature of the nutrient solution to 30-40 ℃;
s5, conveying the nutrient solution from the nutrient solution preparation tank to a transfer tank;
s6, putting the ginseng seeds into a transfer tank under the aseptic operation environment, culturing for 5-10 days at the temperature of 27.5-28.5 ℃, and continuously introducing oxygen for aeration at the aeration pressure of 0.15-0.35Mpa during the culture period;
s7, transferring the culture solution and the ginseng seeds in the transfer tank to a culture tank for culture, culturing for 65-80 days at the temperature of 27.5-28.5 ℃, and continuously introducing oxygen into the transfer tank for aeration at the aeration pressure of 0.15-0.35 Mpa;
s8, discharging the culture solution in the culture tank, and taking out the germinated ginseng seedlings.
2. The method for aseptic culture of ginseng seeds according to claim 1, wherein: the high-temperature sterilization methods of the interiors of the sterile water storage tank, the transfer tank and the culture tank are steam sterilization, steam is introduced from the tops of the sterile water storage tank, the transfer tank and the culture tank, and is discharged from the bottoms of the transfer tank and the culture tank for sterilization; the sterilization method of the nutrient solution preparation tank comprises the following steps: introducing steam into an outer jacket of the nutrient solution preparation tank for heating, introducing sterile oxygen into the bottom of the nutrient solution preparation tank and discharging from the top, then closing the top for exhausting, introducing steam from the top and discharging gas in the nutrient solution preparation tank from the bottom in a replacement manner.
3. The method for aseptic culture of ginseng seeds according to claim 2, wherein: the heating sterilization mode of the nutrient solution in the nutrient solution preparation tank is as follows: introducing steam into an outer jacket of the nutrient solution preparation tank, introducing the steam into the nutrient solution preparation tank when the temperature in the nutrient solution preparation tank reaches 100 ℃, stopping introducing the steam and keeping the steam when the temperature in the tank reaches 120 ℃, introducing the steam when the temperature in the tank reaches 100 ℃, and circulating for 1.5 hours at the temperature of 100-120-100 ℃.
4. The method for aseptic culture of ginseng seeds according to claim 3, wherein: in the step S6, the ginseng seeds are placed in the transfer tank under the aseptic environment in a manner that: utilize a workstation cover of taking the operation hole to detain in the upper end of shifting the jar, the upper end of shifting the jar is provided with puts into the mouth, sets up in the workstation and utilizes the ultraviolet lamp sterilization more than 12 hours, then the alcohol disinfection of operator's both hands, to the outer mouthful disinfection of the household utensils of splendid attire ginseng seed, the operator puts into the household utensils of splendid attire ginseng seed from the operation hole and carries out ultraviolet sterilization minimum 20min, then pours the ginseng seed into in the shifting jar.
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| CN2754377Y (en) * | 2004-05-31 | 2006-02-01 | 吴甲申 | Household high-efficient continuous integrated producing apparatus for edible mushroom liquid bacteria |
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