CN101142917A - Method for improving halophyte seed germinating potential and its special-purpose soaking liquid - Google Patents

Method for improving halophyte seed germinating potential and its special-purpose soaking liquid Download PDF

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Publication number
CN101142917A
CN101142917A CNA2007101211264A CN200710121126A CN101142917A CN 101142917 A CN101142917 A CN 101142917A CN A2007101211264 A CNA2007101211264 A CN A2007101211264A CN 200710121126 A CN200710121126 A CN 200710121126A CN 101142917 A CN101142917 A CN 101142917A
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seed
halophyte
halophytes
gibberellin
water
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CN100536660C (en
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冯固
张士荣
宋杰
张福锁
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China Agricultural University
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China Agricultural University
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Abstract

The invention discloses a method for improvement of halophyte seed germinating energy and a special soak solution. The method is that the seed of the halophyte is soaked in mixed water solution consisting of a sodium chloride, a calcium chloride, a gibberellins and water for 2-7 days; the weight ratio of the sodium chloride, calcium chloride, the gibberellins and the water is 12000-58000:500-1000:5-15:1000 in serial. The method provided by the invention can effectively improve germinating energy of the halophyte seed, stimulate vigor of the seed, induce rapid and neat germination of the seed and solve a key bottleneck problem in saline soil agricultural production, with important practical value. The method provided by the invention has simple operation and easy availability of the necessary ingredient and can be widely used in saline soil agricultural production for planting of the halophyte in the way of sowing seed.

Description

A kind of method and special-purpose soaking liquid thereof that improves halophyte seed germinating potential
Technical field
The present invention relates to improve the method and the soak thereof of plant seed germination gesture, particularly relate to a kind of method and special-purpose soaking liquid thereof that improves halophyte seed germinating potential.
Background technology
Halophytes is meant to surpass in the environment of 3.3bar at salt solution osmotic pressure and grows, and finishes the general designation of the plant of its history of life.Halophytes has the diversified economy value, for example as energy-source plant, medicinal plant etc.With the halophytes artificial cultivation is that the solonchak agricultural of core has become the novel ecological industry of a worldwide class.
Halophytes belongs to wild plant, for adapted soil salinity and the violent natural environment of moisture content change, the seed of this class plant has all formed a kind of ecological and biological characteristic of sprouting little by little, in batch, the risk that the population that may cause to share, to reduce neat sprouting of seed destroys.The seed germination time of halophytes can continue a week, even more than 10 days, 3 days germination vigor is lower than 60%, even lower.The characteristic that halophytic seed can not neatly be sprouted has been brought very for its artificial planting and is seriously influenced, and direct result causes the disconnected ridge that is short of seedling exactly, and perhaps plant size, breeding time are inconsistent, have a strong impact on field management, population growth, output and benefit.In order to overcome this difficult problem, forefathers study it from different perspectives.For example, be derivant with gibberellin, (Hua Chun etc., external source gibberellin are to the influence of Salicornia bigelovii seed germination and growth of seedling under the salt stress, Nanjing Normal University's journal natural science edition, 2007,30 (1): 82-87) to break halophytes (extra large covering) seed dormancy.Zhao Kefu etc. have studied a kind of anti-salt drought resistant agent for plant and using method thereof, they press the special ratios prescription with gibberellin, salicylic acid, amino oligosaccharide (O-CMC) or their derivative and calcium salt, be used for soaking seed before the plant seeding, or seedling stage foliage-spray, improve the comprehensive salt tolerant drought-resistance ability of plant (Chinese patent application number 99112463.4).David H.Holloway has invented " seed germination medium (Seed germination medium) ", be characterized in the material of the face tissue (tissue paper) that adopts a kind of good permeability and scribble the wax waterproof material and a kind of good water absorption, seed is clipped between two kinds of materials, the face tissue of coated with waterproof material is positioned at top, can guarantee the gas permeability in the seed germination process.This method can guarantee seed normally sprouting (U.S. Patent number 6681521) in salt environment.The in addition Australian patent application AU-A-81394/75 similar to this patent of invention, french patent application FR-A-2440438 etc.But still there is not the technical scheme that effectively addresses this problem at present.
Summary of the invention
The purpose of this invention is to provide a kind of method and special-purpose soaking liquid thereof that improves halophyte seed germinating potential, to solve the problem that seed can not be sprouted simultaneously in the halophytes cultivation.
The soak of raising halophyte seed germinating potential provided by the invention, the mixed aqueous solution of forming by sodium chloride, calcium chloride, gibberellin and water; The ratio of weight and number of described sodium chloride, calcium chloride, gibberellin and water is followed successively by 12000-58000: 500-1000: 5-15: 1000000.
The method of raising halophyte seed germinating potential provided by the invention is that the seed with halophytes soaked 2-7 days in the mixed aqueous solution of being made up of sodium chloride, calcium chloride, gibberellin and water; The ratio of weight and number of described sodium chloride, calcium chloride, gibberellin and water is followed successively by 12000-58000: 500-1000: 5-15: 1000000.
For reinforced effects, in the immersion process of seed, keep bubbling air in solution.The amount of described bubbling air is 2-5cm -3/ liter. minute.
In immersion process the temperature of solution remain on 10 ℃-25 ℃ comparatively useful.
Method of the present invention all is suitable for for all halophytess, for example the Li Ke halophytes.The representational genus of Li Ke halophytes is Suaeda (Suaeda), sacsaoul (Haloxylon), salicornia europaeal genus (Salicornia), Salsola (Salsola), Atriplex (Atriplex).
Method of the present invention can effectively improve the germination vigor of halophytic seed, excites seed vitality, induces quick, the neat sprouting of seed, has solved the bottleneck problem of a key in the solonchak agricultural production, has important application value.Method of the present invention is easy and simple to handle, and required batching obtains easily, can be widely used in the solonchak agricultural production of planting halophytes in the planting seed mode, is applicable to plantation of landlocked saline soil earth halophytes and the seawater agricultural production of coastal tidal area.
Embodiment
The sprouting experiment of embodiment 1, cystocarp alkali fluffy (Suaeda physophora) seed
Take by weighing edible NaCl41 gram, CaCl 28 milligrams of 1 gram, gibberellin are dissolved in 1 kilogram of drinking water successively.Add the fluffy seed of cystocarp alkali in mixed solution, the amount of the fluffy seed of adding cystocarp alkali can be flooded by solution with seed and is as the criterion.Seed soaking is 3 days under 20 ℃ condition, ventilates in solution with air pump all the time in the seed soaking process, and the amount of ventilation is 2-5cm -3/ liter. minute.After the seed soaking seed is pulled out, sowed.The germination rate of seed after planting in 1 day can reach more than 90%.
The sprouting experiment of embodiment 2, sacsaoul (Haloxylon ammodendron) seed
Take by weighing edible NaC112 gram, CaCl 215 milligrams of 1 gram, gibberellin are dissolved in 1 kilogram of drinking water successively.Add the sacsaoul seed in mixed solution, the amount of adding sacsaoul seed can be flooded by solution with seed and is as the criterion.Seed soaking is 7 days under 25 ℃ condition, ventilates in solution with air pump all the time in the seed soaking process, and the amount of ventilation is 2-5cm -3/ liter. minute.After the seed soaking seed is pulled out, sowed.The germination rate of seed after planting in 1 day can reach more than 90%.
The sprouting experiment of embodiment 3, Herba Atriplicis centralasiaticae (Atriplex centralasiatica) seed
Take by weighing edible NaC124.4 gram, CaCl 20.5 5 milligrams of gram, gibberellin are dissolved in 1 kilogram of drinking water successively.Add the Herba Atriplicis centralasiaticae seed in mixed solution, the amount of adding Herba Atriplicis centralasiaticae seed can be flooded by solution with seed and is as the criterion.Seed soaking is 2 days under 25 ℃ condition, ventilates in solution with air pump all the time in the seed soaking process, and the amount of ventilation is 2-5cm -3/ liter. minute.After the seed soaking seed is pulled out, sowed.The germination rate of seed after planting in 1 day can reach more than 90%.
The sprouting experiment of embodiment 4, sea, North America covering (also claiming the North America salicornia europaeal, Salicornia bigelovii) seed
Take by weighing edible NaCl58 gram, CaCl 210 milligrams of 1 gram, gibberellin are dissolved in 1 kilogram of drinking water successively.Add sea, North America covering seed in mixed solution, the amount of sea, adding North America covering seed can be flooded by solution with seed and is as the criterion.Seed soaking is 4 days under 10 ℃ condition.After the seed soaking seed is pulled out, sowed.The germination rate of seed after planting in 2 days can reach more than 90%.
The sprouting experiment of embodiment 5, Suaeda salsa (Suaeda salsa) seed
Take by weighing edible NaCl29.3 gram, CaCl 210 milligrams of 1 gram, gibberellin are dissolved in 1 kilogram of drinking water successively.Add the Suaeda salsa seed in mixed solution, the amount of adding Suaeda salsa seed can be flooded by solution with seed and is as the criterion.Seed soaking is 3 days under 20 ℃ condition.After the seed soaking seed is pulled out, sowed.The germination rate of seed after planting in 1 day can reach more than 90%.

Claims (9)

1. soak that improves halophyte seed germinating potential, the mixed aqueous solution of forming by sodium chloride, calcium chloride, gibberellin and water; The ratio of weight and number of described sodium chloride, calcium chloride, gibberellin and water is followed successively by 12000-58000: 500-1000: 5-15: 1000000.
2. method that improves halophyte seed germinating potential is that the seed with halophytes soaked 2-7 days in the mixed aqueous solution of being made up of sodium chloride, calcium chloride, gibberellin and water; The ratio of weight and number of described sodium chloride, calcium chloride, gibberellin and water is followed successively by 12000-58000: 500-1000: 5-15: 1000000.
3. method according to claim 2 is characterized in that: in the immersion process of seed, keep bubbling air in solution.
4. method according to claim 3 is characterized in that: the amount of described bubbling air is 2-5cm -3/ liter. minute.
5. according to claim 2 or 3 or 4 described methods, it is characterized in that: the temperature of solution is 10 ℃-25 ℃ in the described immersion process.
6. method according to claim 2 is characterized in that: sowing at once after the seed after the immersion is pulled out.
7. according to claim 2 or 3 or 4 or 6 described methods, it is characterized in that: described halophytes is the Li Ke halophytes.
8. method according to claim 7 is characterized in that: described Li Ke halophytes is the plant of Suaeda, sacsaoul, salicornia europaeal genus, Salsola or Atriplex.
9. method according to claim 8 is characterized in that: described Li Ke halophytes is that cystocarp alkali is fluffy, sacsaoul, sea, North America covering, Herba Atriplicis centralasiaticae or Suaeda salsa.
CNB2007101211264A 2007-08-30 2007-08-30 Method for improving halophyte seed germinating potential and its special-purpose soaking liquid Expired - Fee Related CN100536660C (en)

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Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102919051A (en) * 2012-12-04 2013-02-13 中国科学院新疆生态与地理研究所 Method for quickly reducing sulphur-containing waste water pollution by utilizing halophilous plant
CN104145561A (en) * 2014-08-01 2014-11-19 兰州大学 Method for atriplex canescens seed germination
CN104686008A (en) * 2015-03-10 2015-06-10 塔里木大学 Method for breaking chenopodiaceae plant seed dormancy
CN104824620A (en) * 2015-04-29 2015-08-12 潍坊友容实业有限公司 Method for extracting middle-low sodium plant salts from suaeda salsa planted in moderately and heavily-alkalized saline-alkali soil
CN104872613A (en) * 2015-04-29 2015-09-02 潍坊友容实业有限公司 Extraction method of plant salt with high content of microelements from suaeda salsa
CN105052291A (en) * 2015-09-02 2015-11-18 常州市长宇实用气体有限公司 Method for improving salt and alkali soil through halophyte
CN109392353A (en) * 2018-12-14 2019-03-01 扬州大学 A kind of production method being enriched with glucosinolate in Vegetables in Brassica sprout
CN109548584A (en) * 2019-01-16 2019-04-02 山东农业大学 A method of promote reed seed to sprout and improve salt tolerance
CN112005658A (en) * 2020-09-03 2020-12-01 青岛农业大学 Method for determining salt tolerance of quinoa seeds in germination period
CN112005657A (en) * 2020-09-03 2020-12-01 青岛农业大学 Method for screening salt-tolerant germplasm of quinoa by utilizing natural seawater
CN113455309A (en) * 2021-07-27 2021-10-01 常州天大龙成节能环保科技有限公司 Method for artificially increasing germination rate of natural wild halophytic vegetable seeds
CN114617042A (en) * 2022-03-28 2022-06-14 中国农业科学院棉花研究所 Method for improving drought resistance of cotton by using NaCl pretreatment

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102919051A (en) * 2012-12-04 2013-02-13 中国科学院新疆生态与地理研究所 Method for quickly reducing sulphur-containing waste water pollution by utilizing halophilous plant
CN104145561A (en) * 2014-08-01 2014-11-19 兰州大学 Method for atriplex canescens seed germination
CN104686008A (en) * 2015-03-10 2015-06-10 塔里木大学 Method for breaking chenopodiaceae plant seed dormancy
CN104824620A (en) * 2015-04-29 2015-08-12 潍坊友容实业有限公司 Method for extracting middle-low sodium plant salts from suaeda salsa planted in moderately and heavily-alkalized saline-alkali soil
CN104872613A (en) * 2015-04-29 2015-09-02 潍坊友容实业有限公司 Extraction method of plant salt with high content of microelements from suaeda salsa
CN105052291A (en) * 2015-09-02 2015-11-18 常州市长宇实用气体有限公司 Method for improving salt and alkali soil through halophyte
CN105052291B (en) * 2015-09-02 2017-01-25 中国科学院寒区旱区环境与工程研究所 Method for improving salt and alkali soil through halophyte
CN109392353A (en) * 2018-12-14 2019-03-01 扬州大学 A kind of production method being enriched with glucosinolate in Vegetables in Brassica sprout
CN109548584A (en) * 2019-01-16 2019-04-02 山东农业大学 A method of promote reed seed to sprout and improve salt tolerance
CN112005658A (en) * 2020-09-03 2020-12-01 青岛农业大学 Method for determining salt tolerance of quinoa seeds in germination period
CN112005657A (en) * 2020-09-03 2020-12-01 青岛农业大学 Method for screening salt-tolerant germplasm of quinoa by utilizing natural seawater
CN113455309A (en) * 2021-07-27 2021-10-01 常州天大龙成节能环保科技有限公司 Method for artificially increasing germination rate of natural wild halophytic vegetable seeds
CN114617042A (en) * 2022-03-28 2022-06-14 中国农业科学院棉花研究所 Method for improving drought resistance of cotton by using NaCl pretreatment

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