CN102523786A - Preparation method for novel tobacco seed germination bed - Google Patents
Preparation method for novel tobacco seed germination bed Download PDFInfo
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- CN102523786A CN102523786A CN2011104445161A CN201110444516A CN102523786A CN 102523786 A CN102523786 A CN 102523786A CN 2011104445161 A CN2011104445161 A CN 2011104445161A CN 201110444516 A CN201110444516 A CN 201110444516A CN 102523786 A CN102523786 A CN 102523786A
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Abstract
The invention provides a preparation method for a novel tobacco seed germination bed. According to the method, high water absorption resin poly(2-acryloylamino-2-methylpropanesulfonic acid) (PAMPS) powder absorbs water in a culture dish to prepare the novel tobacco seed germination bed. The novel tobacco seed germination bed prepared by the method of the present invention has the following characteristics that: the bed shows the hydrogel state; the tobacco seeds can be directly placed on the gelatinous germination bed, the seeds directly absorb the water from the hydrogel during the whole germination process, such that the regular water supplement is not required; the germination bed of the present invention has good water retention property, such that the moisture does not easily volatile, and the uniform germination condition can be provided.
Description
Technical field
The present invention relates to the agronomic crop seed germination detection technique, be specifically related to a kind of can the repeated use and the preparation method of the novel tobacco seed sprouting bed that whole process need not be watered.
Background technology
Germination test is under laboratory's particular environment condition, to measure the maximum germination potentiality of seed lot.Quality that in view of the above can more different seed lots.Also can estimate field sowing is worth.Seed management department carries out the experiment of germinateing, and can in time hit the production and operation behavior of fake and inferior seed.Significant to guaranteeing agricultural production safety, also play an important role to regulating the seed quality dispute.
Seed germination experiment needs specific germinating bed, and at present, paper bed (germination paper, filter paper etc.) is to use one type of maximum germinating bed (opening spring Khanh Hoa Wang Jianhua, 2005) in the seed germination experiment.Problem is that the water retention capacity of paper bed is relatively poor, needs regular replenishment moisture.Simultaneously, the paper bed need be contained in the containers such as culture dish or germination box, because the sealing of container is different, causes the speed of moisture evaporation in the different paper beds different, is difficult to guarantee the uniformity of all germinating beds aspect water content.In addition, the paper bed will abandon after using once, can not reuse.
Summary of the invention
The objective of the invention is to avoid the deficiency of above-mentioned prior art, provide a kind of and can reuse and the preparation method of the novel tobacco seed sprouting bed that whole process need not be watered.The germinating bed for preparing is the water-setting glue, gathers 2-acrylamido-2-methyl propane sulfonic acid (PAMPS) powder by high hydroscopic resin and in culture dish, absorbs water back the preparation.Seed can directly be placed on the gelatinous germinating bed, and whole germination process seed directly absorbs water from hydrogel, therefore, does not need regular replenishment moisture.In addition, gather 2-acrylamido-2-methyl propane sulfonic acid and have good water retention property, moisture is not volatile, and more consistent germination condition can be provided.
A kind of preparation method of novel tobacco seed sprouting bed may further comprise the steps:
1): N
2Protection adds 100 g deionized waters, 35 g 2-acrylamido-2-methyl propane sulfonic acids, 12 g sodium hydroxide, 0.014 g potassium peroxydisulfate and 0.105 g N at 500 ml round-bottomed flasks down successively; N '-60 ℃ of heat temperature raisings of methylene-bisacrylamide are answered 12 h; 60 ℃ of drying 48 h of product, crushing screening (100 order) obtains gathering about 45 g of 2-acrylamido-2-methyl propane sulfonic acid powder.
2): a certain amount of 2-of gathering acrylamido-2-methyl propane sulfonic acid powder is dissolved in the deionized water, stirs, the solution concentration that obtains is 0.1%;
3): the solution of 10～100mL step 2 preparation is poured in the germination box or culture dish of different size, left standstill 24 h and obtain gelatinous high hydroscopic resin germinating bed.
Wherein, said germination box comprises the plastics germination box of 12 * 12cm and 24 * 12cm, and culture dish comprises glass or the plastic culture dish of diameter 6cm～18cm.
Wherein, if give thousand kernel weight when the minimum grain tobacco seed of 0.05-0.5g germinates, the thickness of bud bed is 0.2 ~ 0.5cm.
The thickness of setting the bud bed is the growing state for the ease of the root of observing the back seed that germinates.
Have following advantage with respect to prior art the present invention:
1): the core material that the present invention uses gathers the raw material preparing that 2-acrylamido-2-methyl propane sulfonic acid uses relative low price; The preparation method is simple and easy; Gathering 2-acrylamido-2-methyl propane sulfonic acid hydrogel and after drying, can absorb water once more after the use, use repeatedly.
2): gather 2-acrylamido-2-methyl propane sulfonic acid hydrogel and be transparence, can clearly observe the metamorphosis of root in the process of growth.
3): gather 2-acrylamido-2-methyl propane sulfonic acid and have good retaining water retaining function, therefore, whole germination process does not need regular replenishment moisture, can save a large amount of manpowers.
Embodiment
Among the following embodiment, the seed of tobacco bred " the big gold dollar of safflower " and " MS K326 " is from China Tobacco Seed Yuxi Co., Ltd..
Embodiment 1The preparation of high hydroscopic resin germinating bed
N
2Protection adds 100 g deionized waters, 35 g 2-acrylamido-2-methyl propane sulfonic acids, 12 g sodium hydroxide, 0.014 g potassium peroxydisulfate and 0.105 g N at 500 ml round-bottomed flasks down successively; N '-60 ℃ of heat temperature raisings of methylene-bisacrylamide are answered 12 h; 60 ℃ of drying 48 h of product, crushing screening (100 order) obtains gathering about 45 g of 2-acrylamido-2-methyl propane sulfonic acid powder.Get and gather 2-acrylamido-2-methyl propane sulfonic acid powder 0.02 g, add 20 ml deionized waters, stir and pour in the 9 cm culture dishes, leave standstill 24 h and obtain the high hydroscopic resin germinating bed that thickness is 0.3cm.
?
Embodiment 2Seed germination experiment
Get 100 in the big gold dollar seed of safflower, 4 repetitions evenly place the 9 cm culture dishes that contain suction back high hydroscopic resin of embodiment 1 preparation.Other gets 100 big gold dollar seeds of safflower, and 4 repetitions evenly place the 9 cm culture dishes that are lined with two-layer moistening filter paper as contrast (CK).Then in illumination box 30 ℃ of (8 h) nights of daytime 20 ℃ of (16 h) alternating temperatures, 16 d that germinate.Add 1 ml deionized water in the paper bed culture dish every day and keep filter paper moistening, the high hydroscopic resin germinating bed no longer keeps the skin wet.Write down the chitting piece number every day, the 16th d calculates germination rate, germination index (GI) and vitality index (VI), computing formula: GI=∑ (Gt/Dt), and VI=S * GI, in the formula: Gt is t days a chitting piece number, and Dt is the germination number of days, and S is full stand long (mm).
Embodiment 3Seed germination experiment
Repeat embodiment 2, following difference is arranged: change the big gold dollar seed of safflower into MS K326 seed.
Embodiment 4The growth of seedling test
Get 100 in the big gold dollar seed of safflower, 4 repetitions evenly place the 9 cm culture dishes that contain suction back high hydroscopic resin of embodiment 1 preparation.Other gets 100 big gold dollar seeds of safflower, and 4 repetitions evenly place the 9 cm culture dishes that are lined with two-layer moistening filter paper as contrast (CK).20 ℃ of (16 h) alternating temperatures are cultivated 30 d 30 ℃ of (8 h) nights of daytime in illumination box then.Add 1 ml deionized water in the paper bed culture dish every day and keep filter paper moistening, the high hydroscopic resin germinating bed no longer keeps the skin wet.Get the seedling of growth 30 d, blot with filter paper with running water flushing back.Each handles picked at random 30 strains, and the length with ruler its root of measurement and overground part is accurate to mm; With one thousandth sensibility reciprocal balance weighing full stand fresh weight, seedling is dried 24 h down at 80 ℃, measure the full stand dry weight, be accurate to mg.
?
Embodiment 5The growth of seedling test
Repeat embodiment 4, following difference is arranged: change the big gold dollar seed of safflower into MS K326 seed.
Big gold dollar of safflower and MS K326 seed place on the prepared water-absorbing resin of embodiment 1 and germinate, and result of the test is carried out
tTest (α=0.05) shows that seed germination rate, germination index and vitality index are compared with contrast (germinateing on the paper) does not all have significant difference (table 1, table 2).The big gold dollar seed of safflower is grown on the prepared water-absorbing resin of embodiment 1, and the seedling root is long, full stand is long, the full stand fresh weight is compared (table 3) with the full stand dry weight with contrast (growing on the paper) does not all have significant difference; MS K326 seed is grown on the prepared water-absorbing resin of embodiment 1, and the seedling root is long significantly to be lower than contrast, does not all have significant difference (table 4) and the seedling overground part is high, the full stand fresh weight is compared with contrast with the full stand dry weight.
The variation of the big gold dollar seed of table 1 tobacco bred safflower germination rate, germination index and vitality index on different germinating beds
| Germination condition | Germination rate (%) | Germination index (GI) | Vitality index (VI) |
| Germinate on the paper (CK) | 96.7a * | 17.72a | 27.12a |
| Water-absorbing resin **Last germination | 96.3a | 16.58a | 25.83a |
*Lowercase represent between different disposal the significance of difference relatively (α=0.05, LSD), down with.
*What water-absorbing resin referred to 0.1% concentration gathers 2-acrylamide-2-methyl propane sulfonic acid (PAMPS), down with.
The variation of table 2 tobacco bred MS K326 seed germination rate, germination index and vitality index on different germinating beds
| Germination condition | Germination rate (%) | Germination index (GI) | Vitality index (VI) |
| On the paper (CK) | 92.7a * | 16.49a | 17.58a |
| Water-absorbing resin ** | 92.0a | 16.27a | 16.98a |
The big gold dollar of table 3 tobacco bred safflower grows on different germinating beds that root is long, overground part is high, full stand fresh weight and full stand dry weight change
Table 4 tobacco bred MS K326 grows on different germinating beds that root is long, overground part is high, full stand fresh weight and full stand dry weight change
The seed sprouting bed of the present invention's preparation except the growth to the seedling root has remarkable inhibitory action, does not all make significant difference to the growth of seedling overground part, seedling fresh weight and dry weight and seed sprouting situation.Therefore, the prepared seed sprouting bed of the present invention can be used for indexs such as seed germination rate, germination index and vitality index are detected, and the use value of seed lot is assessed, and can satisfy the needs in the production.The core material that the present invention uses gathers 2-acrylamido-2-methyl propane sulfonic acid and utilizes cheap raw material preparing, and the preparation method is simple and easy, and gathering 2-acrylamido-2-methyl propane sulfonic acid hydrogel and after drying, can absorb water once more after the use used repeatedly.Gather 2-acrylamido-2-methyl propane sulfonic acid hydrogel and be transparence, can clearly observe the metamorphosis of root in the process of growth.In addition, gather 2-acrylamido-2-methyl propane sulfonic acid and have good retaining water retaining function, therefore, whole germination process does not need regular replenishment moisture, can save a large amount of manpowers.
Claims (3)
1. the preparation method of a novel tobacco seed sprouting bed may further comprise the steps:
1): N
2Protection adds 100 g deionized waters, 35 g 2-acrylamido-2-methyl propane sulfonic acids, 12 g sodium hydroxide, 0.014 g potassium peroxydisulfate and 0.105 g N at 500 ml round-bottomed flasks down successively; N '-60 ℃ of heat temperature raisings of methylene-bisacrylamide are answered 12 h; 60 ℃ of drying 48 h of product pulverized 100 mesh sieves and obtained gathering about 45 g of 2-acrylamido-2-methyl propane sulfonic acid powder;
2): a certain amount of 2-of gathering acrylamido-2-methyl propane sulfonic acid powder is dissolved in the deionized water, stirs, the solution concentration that obtains is 0.1%;
3): the solution of 10～100mL step 2 preparation is poured in the germination box or culture dish of different size, left standstill 24 h and obtain gelatinous high hydroscopic resin germinating bed.
2. preparation method as claimed in claim 1 is characterized in that said germination box comprises the plastics germination box of 12 * 12cm and 24 * 12cm, and culture dish comprises glass or the plastic culture dish of diameter 6cm～18cm.
3. preparation method as claimed in claim 1 is characterized in that, if give thousand kernel weight when the minimum grain tobacco seed of 0.05 ~ 0.5g germinates, the thickness of bud bed is 0.2 ~ 0.5cm.
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| CN2011104445161A CN102523786B (en) | 2011-12-27 | 2011-12-27 | Preparation method for novel tobacco seed germination bed |
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| CN2011104445161A CN102523786B (en) | 2011-12-27 | 2011-12-27 | Preparation method for novel tobacco seed germination bed |
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| CN102523786B CN102523786B (en) | 2013-11-20 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102726147A (en) * | 2012-07-12 | 2012-10-17 | 贵州省烟草科学研究所 | Tobacco seed germinating bed and preparation method thereof |
| CN109503779A (en) * | 2018-11-28 | 2019-03-22 | 河南农业大学 | A method of Tobacco Seed Germination bed is prepared using tabacco straw |
| CN109575200A (en) * | 2018-11-28 | 2019-04-05 | 河南农业大学 | A method of Tobacco Seed Germination bed is prepared using corn stover |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109593169A (en) * | 2018-11-28 | 2019-04-09 | 河南农业大学 | A method of Tobacco Seed Germination bed is prepared using offal |
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| EP0494820A1 (en) * | 1991-01-10 | 1992-07-15 | Societe Nationale D'exploitation Industrielle Des Tabacs Et Allumettes | Improvements for the production of tobacco plants using the flotation system |
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| CN201766827U (en) * | 2010-08-13 | 2011-03-23 | 云南省烟草农业科学研究院 | Germination test bed for small plant seeds |
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2011
- 2011-12-27 CN CN2011104445161A patent/CN102523786B/en active Active
Patent Citations (3)
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| EP0494820A1 (en) * | 1991-01-10 | 1992-07-15 | Societe Nationale D'exploitation Industrielle Des Tabacs Et Allumettes | Improvements for the production of tobacco plants using the flotation system |
| CN101611663A (en) * | 2009-07-24 | 2009-12-30 | 中国烟草总公司黑龙江省公司牡丹江烟草科学研究所 | A kind of tobacco seed germination accelerating method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102726147A (en) * | 2012-07-12 | 2012-10-17 | 贵州省烟草科学研究所 | Tobacco seed germinating bed and preparation method thereof |
| CN102726147B (en) * | 2012-07-12 | 2013-12-11 | 贵州省烟草科学研究所 | Tobacco seed germinating bed and preparation method thereof |
| CN109503779A (en) * | 2018-11-28 | 2019-03-22 | 河南农业大学 | A method of Tobacco Seed Germination bed is prepared using tabacco straw |
| CN109575200A (en) * | 2018-11-28 | 2019-04-05 | 河南农业大学 | A method of Tobacco Seed Germination bed is prepared using corn stover |
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| CN102523786B (en) | 2013-11-20 |
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