CN103718756B - A kind of storage practice of gathering of Radix Bupleuri seed - Google Patents
A kind of storage practice of gathering of Radix Bupleuri seed Download PDFInfo
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- CN103718756B CN103718756B CN201410029737.6A CN201410029737A CN103718756B CN 103718756 B CN103718756 B CN 103718756B CN 201410029737 A CN201410029737 A CN 201410029737A CN 103718756 B CN103718756 B CN 103718756B
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Abstract
The present invention relates to agricultural technology field, disclose the storage practice of gathering of a kind of Radix Bupleuri seed.The method of the invention is when Radix Bupleuri poppyhead sequence fruit color is yellow, its seed is gone up plant part jointly cut off in the lump, then alignd in the lower end of ground plant part and be bundled into wisp, vertically place in shady and cool ventilation place and dry in the shade, seed is collected after drying in the shade, in the airing of shady and cool ventilation place, after airing, shady and cool ventilation place is deposited in pack.The method of the invention can improve the Maturity regularity of the mass of 1000 kernel of Radix Bupleuri seed, germination rate and same batch of seed of gathering, promote Radix Bupleuri seed embryo rate after gathering, reduce the accumulation etc. of its main germination inhibitor coumarin substances simultaneously, it is integrally improved the sprouting quality of Radix Bupleuri seed, due to existing storage practice of gathering, good basis has been established in the cultivation for Radix Bupleuri.
Description
Technical field
The present invention relates to agricultural technology field, particularly relate to the storage practice of gathering of a kind of Radix Bupleuri seed.
Background technology
One of certified products source of bulk medicinal materials Radix Bupleuri that Radix Bupleuri (BupleurumchinenseDC.) is States Pharmacopoeia specifications, containing saponins, volatile oil, flavonoid and polysaccharide isoreactivity composition, have antibacterial, antipyretic, calm, protect the liver and the multiple efficacies such as immunomodulating.
Radix Bupleuri medicinal history is long, increases with market demand, and supply falls short of demand for wild resource.Current Radix Bupleuri is mainly derived from the artificial growth in each place of production, but its seed germination state is poor, it has also become the bottleneck problem of Radix Bupleuri implant mass.Be in particular in that germination rate is low, thousand grain weigth light, sprout after Seedling length, seed embryo rate is relatively low, plant the aspects such as Intradermal germination inhibitor (having proved to be coumarin substances) is higher, kernel maturing is inconsistent.There are some researches show, remove kind of micromicro during sowing Radix Bupleuri and reduce the germination inhibitor the included depression effect to seed germination, thus improve germination rate (Wei build and etc. the impact [J] that Radix Bupleuri and bupleurum falcatum seed are sprouted by kernel maturing and seed coat. CHINA JOURNAL OF CHINESE MATERIA MEDICA, 2003,7:614-617).Additionally, pass through to change seed soaking water temperature, Germination Temperature, reserve temperature, apply phytohormone, chemical agent and physics radiation etc. and also can improve Radix Bupleuri seed germination rate to a certain extent, but inconvenience is applied in production practices.In addition, build Wei and wait the middle bavin series Radix Bupleuri kind that becomes of selection-breeding have higher germination rate (Zheng Tingting etc. the election effects [J] of the secondary new varieties of Radix Bupleuri " middle bavin 2 " and " middle bavin 3 ". CHINA JOURNAL OF CHINESE MATERIA MEDICA, 2010,15:1931-1934), but in the field introducing a fine variety production is put into practice, find that the need for environment in Radix Bupleuri producing region, various places is adapted to by this series of products for a long time, it is difficult to promote immediately.
It addition, preserve, improper and Radix Bupleuri seed collecting time is improper also can reduce its sprouting quality, and therefore the task of top priority is to provide a kind of ideal method and measure to improve the sprouting quality of Radix Bupleuri seed, and good basis is established in the cultivation for Radix Bupleuri.
Summary of the invention
In view of this, it is an object of the present invention to provide the storage practice of gathering of a kind of Radix Bupleuri seed so that described method improve Radix Bupleuri seed after germination rate, thousand grain weigth, sprouting Seedling length, seed embryo rate, plant the sprouting quality in Intradermal germination inhibitor, kernel maturing etc..
For realizing the purpose of the present invention, the present invention adopts the following technical scheme that
A kind of storage practice of gathering of Radix Bupleuri seed, it is characterized in that, when Radix Bupleuri poppyhead sequence fruit color is yellow, its seed is gone up plant part jointly cut off in the lump, then alignd in the lower end of ground plant part and be bundled into wisp, vertically placing in shady and cool ventilation place and dry in the shade, after drying in the shade, collecting seed, in the airing of shady and cool ventilation place, after airing, shady and cool ventilation place is deposited in pack.
The defect that the present invention preserves according to existing Radix Bupleuri seed collection, in conjunction with suitable agricultural technology on further investigation Radix Bupleuri seed physiology biochemical characteristic, the method providing a set of brand-new storage Radix Bupleuri seed of gathering, makes up existing method defect, improves the sprouting quality of Radix Bupleuri seed.
Wherein, described Radix Bupleuri poppyhead sequence fruit color is 53-67 days after being generally time of yellow Radix Bupleuri full-bloom stage.As preferably, described Radix Bupleuri is 2 years raw Radix Bupleuris or 3 years raw Radix Bupleuris.
As preferably, described wisp diameter is 3cm-8cm, more preferably 5cm；In addition it is also possible to select 4cm, 6cm or 7cm.
As preferably, described in the time of drying in the shade be 12 days-16 days, more preferably 14 days；In addition it is also possible to select 13 days or 15 days.
As preferably, described shady and cool ventilation place temperature is 6.0 DEG C-14.0 DEG C, more preferably 10.0 DEG C；In addition it is also possible to select 7.0 DEG C, 8.0 DEG C, 9.0 DEG C, 11.0 DEG C, 12.0 DEG C or 13.0 DEG C.
As preferably, described shady and cool ventilation place humidity is 40%-75%, more preferably 55%；Further, it is also possible to select 45%, 50%, 60%, 65% or 70%.
As preferably, the thickness of described airing is 0.5cm-1cm, more preferably 0.75cm；In addition it is also possible to select 0.6cm, 0.7cm, 0.8cm or 0.9cm.
As preferably, the described airing time is 10 days-20 days, more preferably 15 days；In addition it is also possible to select 11 days, 12 days, 13 days, 14 days, 16 days, 17 days, 18 days or 19 days.
As preferably, described ground plant part is the above plant part of earth's surface 5cm-10cm, namely stays a 5cm-10cm, more preferably 7.5cm；In addition it is also possible to select 6cm, 7cm, 8cm or 9cm.
The method of existing Radix Bupleuri of gathering approximately as:
When yellow black occurs in seed, blade whole withered and yellow time cane can be sliced off (not staying stake) in the lump together with seed, carry out threshing, the dry in the sun respectively of cane, seed done, properly preserves.
Choose the Radix Bupleuri in same cultivation area, storage of gathering is carried out respectively according to existing method and the inventive method, carry out cultivation sowing again after preserving the identical time simultaneously, statistics Radix Bupleuri seed after germination rate, thousand grain weigth, sprouting Seedling length, seed embryo rate, plant the situation in Intradermal germination inhibitor, kernel maturing etc., result shows, is superior to gather according to existing method the situation of seed of storage according to the gather Radix Bupleuri seed situation in every respect of storage of the inventive method.As germination rate can carry 5%, kernel maturing is more uniform, and embryo rate rate of increase is 49.4% etc..
From above technical scheme, the method of the invention can improve the Maturity regularity of the mass of 1000 kernel of Radix Bupleuri seed, germination rate and same batch of seed of gathering, promote Radix Bupleuri seed embryo rate after gathering, reduce the accumulation etc. of its main germination inhibitor coumarin substances simultaneously, it is integrally improved the sprouting quality of Radix Bupleuri seed, due to existing storage practice of gathering, good basis has been established in the cultivation for Radix Bupleuri.
Detailed description of the invention
The embodiment of the invention discloses the storage practice of gathering of a kind of Radix Bupleuri seed.Those skilled in the art can use for reference present disclosure, is suitably modified technological parameter and realizes.Special needs to be pointed out is, all similar replacements and change apparent to those skilled in the art, they are considered as including in the present invention.The method of the present invention is described already by preferred embodiment, and product as herein described substantially can be modified or suitably change and combination by related personnel in without departing from present invention, spirit and scope, realizes and applies the technology of the present invention.
In order to be further appreciated by the present invention, below in conjunction with embodiment, the storage practice of gathering of a kind of Radix Bupleuri seed provided by the invention is described in detail.As undeclared, present invention test is under equivalent environment, and the Radix Bupleuri adopted is identical planting area, it is ensured that test has comparability
Embodiment 1, sprouting quality control assays
1, test method
The inventive method (is dried in the shade hereinafter referred to as band strain): when Radix Bupleuri poppyhead sequence fruit color is yellow, its seed is gone up plant part jointly cut off in the lump, stay a 7.5cm, then alignd in the lower end of ground plant part and be bundled into wisp, diameter is 5cm, vertically place in shady and cool ventilation place (temperature 10 DEG C, humidity 55%) and dry in the shade for 14 days, seed is collected after drying in the shade, in shady and cool ventilation place (temperature 10 DEG C, humidity 55%) airing 15 days, airing thickness is 0.75cm, and after airing, shady and cool ventilation place (temperature 10 DEG C, humidity 55%) is deposited in pack.
Existing method (hereinafter referred to as direct threshing): when yellow black occurs in seed, blade whole withered and yellow time cane can be sliced off in the lump together with seed, do not stay stake, carry out threshing, the dry in the sun respectively of cane, seed is done, properly preserves.
2, seed single plant yield, empty flat rate and mature ratio are relatively
Taking Radix Bupleuri plant 3 strain of 2 kinds of picking methods respectively, effective fruit ear number, green grain, Huang Li, brown grain, black grain seed number, flat grain number and full grain number are added up in plant division, calculate empty flat rate.Seed single plant yield and full situation result thereof are in Table 1.
The Radix Bupleuri seed single plant yield of table 1 different picking methods, empty flat rate and mature ratio are relatively
As shown in Table 1, the seed amount difference of two kinds of picking methods acquisitions is big, dry in the shade in the seed of percentage recovery gained with strain, the seed ratio that Maturity is high is high, and Maturity difference is less between seed, single plant yield is high, and empty flat rate is little, and the single plant yield of direct threshing method, empty flat rate and Maturity are all not as the present invention.It can thus be appreciated that, experienced by the process staying strain to preserve after adopting, drying in the shade with strain gathers not only can promote that the seed that Maturity is low changes to the seed that Maturity is high, and the flat rate of sky that seed can be reduced, promote the formation of full seed, the Maturity and yield that can increase kind of subpopulation of gathering of namely drying in the shade with strain.
3, thousand grain weigth and sprouting state compare
Analyzing after tested, the mass of 1000 kernel of the Radix Bupleuri seed of 2 kinds of picking methods and sprouting index are as all different in sprouted startup day, average daily germination rate and total germination rate etc..Table 2 lists the Radix Bupleuri thousand grain weigth of different picking methods, germination rate, sprouting startup day, Seedling length and average daily germination rate etc. and sprouts parameter.
The sprouting parameter of the Qing Chuan Radix Bupleuri seed of table 2 different picking methods compares
| Picking methods | Germination rate/% | Sprout start day/d | Average daily germination rate/% | Seedling length/cm after 30 days | Mass of 1000 kernel/g |
| Direct threshing | 10.0 | 15 | 0.77 | 1.00 | 1.082 |
| Band strain is dried in the shade | 15.0 | 14 | 1.15 | 1.62 | 1.163 |
Dry in the shade thousand grain weigth of gathering of band strain is big, germination rate is higher than direct threshing seed 5%, and it is fast to sprout startup, average daily germination rate is also high, seedling quality is good, sprouting the seedling after 30d up to 1.62cm, the every sprouting index of gathering of drying in the shade with strain is superior to the seed that direct threshing is gathered, and drying in the shade with strain gathers can be effectively improved the sprouting quality of seed.
4, seed adopt rear embryo rate change compare
Latter two of gathering Radix Bupleuri seed embryo rate all presents stable ascendant trend.When direct threshing is gathered, embryo rate is only 25.1%, adopts one week after and changes greatly, rises to 28.2%, stable about 31.4% after slowly rising to afterwards 4 weeks；And band strain is dried in the shade and gathered the last fortnight seed still for departing from plant, embryo rate rises rapidly, namely reaches 37.8% after 2 weeks, and rate of increase reaches 49.4%, and after 4 weeks, embryo rate reaches 38.3%, gathers seed far above direct threshing.As can be seen here, Radix Bupleuri the storage of seeds early embryo rate steady growth, drying in the shade with strain gathers can be effectively improved embryo adopt rear growth rate and embryo rate.
5, the accumulation of the germination inhibitor coumarin of seed
Coumarin is the major inhibitor matter of Seeds Germination of Bupleurum chinense DC, its coumarin can be carried out ratio analysis by its frozen section fluorescence power under ultraviolet excitation.The seed coumarin relative amount respectively 767.5AU(direct threshing of two kinds of picking methods) and 704.9AU(band strain dry in the shade).It follows that dry in the shade with strain, after the seed collection gathered, the accumulation of its coumarin content reduces.
Embodiment 2, storage practice of gathering of the present invention
When Radix Bupleuri poppyhead sequence fruit color is yellow, its seed is gone up plant part jointly cut off in the lump, stay a 5cm, then being alignd the lower end of ground plant part and be bundled into wisp, diameter is 3cm, vertically places in shady and cool ventilation place (temperature 6 DEG C, humidity 40%) and dries in the shade for 12 days, seed is collected after drying in the shade, in shady and cool ventilation place (temperature 6 DEG C, humidity 40%) airing 10 days, airing thickness was 0.5cm, and after airing, shady and cool ventilation place (temperature 6 DEG C, humidity 40%) is deposited in pack.
Contrast according to the existing method of embodiment 1 contrast test and direct threshing, all due to existing method in Seedling length, seed embryo rate, kind Intradermal germination inhibitor, kernel maturing etc. after germination rate, thousand grain weigth, sprouting, there is the raising effect equal with embodiment 1.
Embodiment 3, storage practice of gathering of the present invention
When Radix Bupleuri poppyhead sequence fruit color is yellow, its seed is gone up plant part jointly cut off in the lump, stay a 10cm, then being alignd the lower end of ground plant part and be bundled into wisp, diameter is 8cm, vertically places in shady and cool ventilation place (temperature 14 DEG C, humidity 75%) and dries in the shade for 16 days, seed is collected after drying in the shade, in shady and cool ventilation place (temperature 14 DEG C, humidity 75%) airing 20 days, airing thickness was 1cm, and after airing, shady and cool ventilation place (temperature 14 DEG C, humidity 75%) is deposited in pack.
Contrast according to the existing method of embodiment 1 contrast test and direct threshing, all due to existing method in Seedling length, seed embryo rate, kind Intradermal germination inhibitor, kernel maturing etc. after germination rate, thousand grain weigth, sprouting, there is the raising effect equal with embodiment 1.
The explanation of above example is only intended to help to understand method and the core concept thereof of the present invention.It should be pointed out that, for those skilled in the art, under the premise without departing from the principles of the invention, it is also possible to the present invention carries out some improvement and modification, these improve and modify in the protection domain also falling into the claims in the present invention.
Claims (4)
1. the storage practice of gathering of a Radix Bupleuri seed, it is characterized in that, when Radix Bupleuri poppyhead sequence fruit color is yellow, its seed is gone up plant part jointly cut off in the lump, then alignd in the lower end of ground plant part and be bundled into wisp, vertically placing in shady and cool ventilation place and dry in the shade, after drying in the shade, collecting seed, in the airing of shady and cool ventilation place, after airing, shady and cool ventilation place is deposited in pack；
Described shady and cool ventilation place temperature on average is 6.0 DEG C-14.0 DEG C；
Described shady and cool ventilation place humidity is 40%-75%；
The thickness of described airing is 0.5cm-1cm；
The described airing time is 10 days-20 days；
Described ground plant part is the above plant part of earth's surface 5cm-10cm.
2. method according to claim 1, it is characterised in that described Radix Bupleuri is 2 years raw Radix Bupleuris or 3 years raw Radix Bupleuris.
3. method according to claim 1, it is characterised in that described wisp diameter is 3cm-8cm.
4. method according to claim 1, it is characterised in that described in the time of drying in the shade be 12 days-16 days.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20040108387A (en) * | 2004-11-30 | 2004-12-23 | 방계룡 | Rice of absorbed herb medicine |
| CN101647356A (en) * | 2008-08-15 | 2010-02-17 | 王安合 | Method for cultivating bupleurum |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20040108387A (en) * | 2004-11-30 | 2004-12-23 | 방계룡 | Rice of absorbed herb medicine |
| CN101647356A (en) * | 2008-08-15 | 2010-02-17 | 王安合 | Method for cultivating bupleurum |
Non-Patent Citations (5)
| Title |
|---|
| 北柴胡在白城地区的驯化栽培研究;谢志明 等;《现代农业科技》;20091231(第4期);第22页 * |
| 北柴胡的高产栽培技术;张春郁;《河南中医学院学报》;20090131;第24卷(第1期);第50-51页 * |
| 柴胡的栽培技术;刘丽芬;《吉林农业》;20091130(第237期);第42-43页 * |
| 柴胡的栽培方法;王凤杰 等;《特种经济动植物》;20091231;第34页 * |
| 药用植物北柴胡、银柴胡的种植技术;罗铭莲 等;《青海农技推广》;20031231(第3期);第55-56页 * |
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