CN102262032B - Method for adjusting water stress chlorophyll and electric conductivity of festuca arundinacea by adopting lawn nursery strip - Google Patents
Method for adjusting water stress chlorophyll and electric conductivity of festuca arundinacea by adopting lawn nursery strip Download PDFInfo
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- CN102262032B CN102262032B CN 201110106089 CN201110106089A CN102262032B CN 102262032 B CN102262032 B CN 102262032B CN 201110106089 CN201110106089 CN 201110106089 CN 201110106089 A CN201110106089 A CN 201110106089A CN 102262032 B CN102262032 B CN 102262032B
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Abstract
The invention discloses a method for adjusting the water stress chlorophyll and the electric conductivity of festuca arundinacea by adopting a lawn nursery strip. The method comprises the following steps of: adding dried natural soil into a container by a potting method by taking waste newspaper and waste cotton cloth as a lower layer of a lawn nursery strip building carrier and taking waste gauze and waste face tissue as an upper layer of the lawn nursery strip building carrier; then paving the lawn nursery strip on the natural soil, and after seeds are planted, keeping a certain amount of water required by seed germination until the seeds germinate; after the seeds germinate for 5 days, starting to stress, and controlling the water content of the soil to be 35 to 50 percent of the saturated water-holding capacity; and after the seeds germinate for 40 days, measuring all physiological and biochemical indexes of festuca arundinacea seedlings, cutting the overground parts of the festuca arundinacea seedlings, keeping culturing for 40 days and harvesting eddish and the underground parts of the festuca arundinacea seedlings, wherein the total culture period lasts 80 days, the average temperature is 18 to 22 DEG C, and the average humidity is 30 to 40 percent. A test result shows that the waste lawn nursery strip exerts a great adjustment function in growth of grasses in a lawn when the plants are subjected to water stress.
Description
Technical field
The invention belongs to environmental protection technical field, relate to adopting and plant the method that band adjusting Festuca Arundinacea water is coerced chlorophyll and conductivity of giving birth to.
Background technology
Along with being on the rise of shortage of water resources, on the urban green space, how to utilize economically water resource to become a hot research problem.Water is the important component part of plant, is that long-term arid or the water deficit of short time all can bring unfavorable even injurious effects to growing of plant.Current urban lawn greenery area constantly increases, and its problem of water consumption also just more and more receives publicity.Lawn tradition water saving mode is that the methods such as selection by drought-resistant variety seed selection, the economic irrigation volume in lawn and irrigation method realize.At present, the application of water-loss reducer and water keeping fertilizer has improved the water saving ability on lawn to a certain extent.Jiao Jinchuan etc. have studied the impact of potash fertilizer on the rye grass drought resistance, and the result shows that certain density potash fertilizer can significantly promote percentage of seedgermination under the drought environment, increases vane thickness and root/shoot ratio, thereby improves the drought-resistant ability of English ryegrass.Exhale daybreak etc. (2005) research to point out, execute that the non-irrigated ability of keeping away of dewdrop grass strengthens behind the phosphorus, low nitrogen improves drought tolerance by osmotic adjustment, and high nitrogen has increased the weight of the water stress degree.
Adopt soverlay technique to have to reduce soil evaporation, improve soil hydro-thermal and nutrient situation, prevent the soil erosion and degeneration, the g and D of promotion crop, reach the purpose that alleviates disaster, it is a time-honored agrotechnical measure.Chinese scholars has been carried out large quantity research to the soil surface soverlay technique, uses this technology in the agricultural production and has obtained obvious effect of increasing production, and in vegetation cover is built, owing to considering the economic benefit reason, seldom use.
Turfgrass survives in the natural open system, and drought stress has become affects one of topmost environmental stress factor of Turfgrass Growth.Moisture is the main ecological cause that determines growth and development of plants, and water deficit or surplus all are directly connected to growing of plant.Since 20th century, people have carried out a large amount of work to the research of arid mechanism.Domestic and international many scholars take the Different stress degree that plant physiology process and biochemical variation that arid causes are inquired into different plants, have determined corresponding investigation index.
Summary of the invention
The present invention selects house refuse discarded object making low cost to plant and gives birth to band, is used for studying the discarded object carrier and plants living planting Festuca Arundinacea, and reach the purpose of regulating Festuca Arundinacea chlorophyll and conductivity; To this, there is no this type of bibliographical information.
For achieving the above object,, the invention provides following technical scheme:
1, a kind of employing is planted and is given birth to the method that band adjusting Festuca Arundinacea water is coerced chlorophyll and conductivity, it is characterized in that being undertaken by following step:
(1) preparation of discarded object planting carrier material
Choose the consistent discarded object in source as the material of cultivating the planting carrier, comprise discarded cotton; Waste newspaper; Discarded gauze; Discarded face tissue; Each material is exposed to the sun a week in the sun, soaked 10 minutes with 1% superoxol, taking-up dries in the sun; Then with waste newspaper and discarded cotton as planting the lower floor of giving birth to the planting carrier, to discard gauze and discarded face tissue as planting the upper strata of giving birth to the planting carrier, 0.5g tall fescue seed is broadcast on the area of the circle of 8 centimetres of diameters and with upper carrier and is fixed, make Festuca Arundinacea and plant living band, to guarantee the homogeneity of seed; This just becomes Festuca Arundinacea and plants living band.
Its fixed form: the boiling water of putting into 100 ℃ for edible wheat flour fully stirs, and makes the paste shape, just makes the carrier fixing agent; The ratio of its wheat flour and water is; 1:10.
(2) test method
Adopt potted plant method, in container, add the original soil after drying, then Festuca Arundinacea is planted to give birth to be with and be tiled on the original soil, after planting guarantee the seed germination water requirement until sprout, after sprouting 5 d, begin to coerce, record each processing and water the water yield every day, soil moisture content is controlled at the 35%-50% of saturation moisture capacity; Sprout after 40 days, measure the various physiological and biochemical indexs of Festuca Arundinacea seedling, and cradle aerial part, continue to cultivate 40 days, results eldish and under ground portion, total cultivation cycle is 80 days, and the laboratory average room temperature is 18-22 ℃, and medial humidity is 30-40%;
(3) index determining
Festuca Arundinacea is cradled, and aerial part and under ground portion are clean with distilled water flushing respectively, and thieving paper blots surface moisture, puts into baking oven, and 1 h that completes under 105 ℃ is dried to constant weight for 80 ℃, measure the content that Festuca Arundinacea water is coerced proline and MDA.
The present invention further discloses discarded object and plant the application of living band aspect adjusting turfgrass growth of Festuca elata.
The good effect that method disclosed by the invention has is: improved the validity of arid area soil turf establishment, its method is to plant the living function of being with adjusting Festuca Arundinacea water to coerce chlorophyll and conductivity by discarded object to realize; And this is the target that is beyond one's reach that existing nonwoven fabrics is planted living band.
Embodiment:
The present invention will be further described below in conjunction with specific embodiment, and following each embodiment only is used for explanation the present invention and is not limitation of the present invention.
Embodiment 1
(1) preparation of discarded object planting carrier material
Choose the consistent discarded object in source as the material of cultivating the planting carrier, comprise discarded cotton; Waste newspaper; Discarded gauze; Discarded face tissue; Each material is exposed to the sun a week in the sun, soaked 10 minutes with 1% superoxol, taking-up dries in the sun; Then with waste newspaper and discarded cotton as planting the lower floor of giving birth to the planting carrier, to discard gauze and discarded face tissue as planting the upper strata of giving birth to the planting carrier, 0.5g tall fescue seed is broadcast on the area of the circle of 8 centimetres of diameters and with upper carrier and is fixed, make Festuca Arundinacea and plant living band, to guarantee the homogeneity of seed;
Its fixed form: the boiling water of putting into 100 ℃ for edible wheat flour fully stirs, and makes the paste shape, just makes the carrier fixing agent; The ratio of its wheat flour and water is; 1:10.
(2) test method
Adopt potted plant method, in container, add the original soil after drying, then Festuca Arundinacea is planted living band and be tiled on the original soil, after planting guarantee the seed germination water requirement until sprout, sprout 5 d after, begin to coerce, record each processing and water the water yield every day, soil moisture content is controlled at 35% of saturation moisture capacity; Sprout after 40 days, measure the various physiological and biochemical indexs of Festuca Arundinacea seedling, and cradle aerial part, continue to cultivate 40 days, results eldish and under ground portion, total cultivation cycle is 80 days, and the laboratory average room temperature is 20 ℃, and medial humidity is 40%;
(3) index determining
Festuca Arundinacea is cradled, and aerial part and under ground portion are clean with distilled water flushing respectively, and thieving paper blots surface moisture, puts into baking oven, and 1 h that completes under 105 ℃ is dried to constant weight for 80 ℃, measure the content that Festuca Arundinacea water is coerced proline and MDA.
Embodiment 2
1 materials and methods
1.1 experiment material
1.1.1 the preparation of discarded object planting carrier material
Choose the consistent discarded object in source as the material of cultivating the planting carrier, comprise discarded cotton, below come reduced representation with C; Waste newspaper below comes reduced representation with N; Discarded gauze below comes reduced representation with G; Discarded face tissue below comes reduced representation with T.Each material is exposed to the sun a week in the sun, soaked 10 minutes with 1% superoxol, taking-up dries in the sun, and plays disinfective action, guarantees the normal sprouting of turfgrass seed.Respectively with waste newspaper and discarded cotton as planting the lower floor of giving birth to the planting carrier, will discarded gauze and discarded face tissue as planting the upper strata of life with the planting carrier, the living belt type of planting that obtains is expressed as respectively C-G, C-T, N-G, N-T.
1.1.2 the preparation of turfgrass seed
The turfgrass of selecting be Festuca Arundinacea (
Festuca arundinaceaL.), simplify with F below.The tall fescue seed seed of selecting full seed, uniformity is for subsequent use.
Plant living assembly method 1.1.3 set up
Turfgrass and discarded object form altogether 4 and plant living planting carrier.Its following being expressed as: CFG, CFT, NFG, NFT.Each processes three repetitions.With original soil plantation tall fescue seed in contrast.
1.1.4 the preparation of matrix soil
This is tested used soil and takes from campus, Tianjin Normal University master school district, chooses the soil block stone before the use, sieves.Dry to constant weight for subsequent use.Recording this native saturation moisture capacity is 43.54%.
1.2 experimental design
Potted plant method is adopted in this experiment, and used container is diameter 8 cm, the cylindrical plastic quality container of high 10 cm.Add the original soil after 200 g are dried in the container.To plant to give birth to be with and be tiled in the original soil upper strata.Control group is directly sowed 0.4 g Festuca Arundinacea and ryegrass seed.
Plant to give birth to be with and refer to: the 0.5g tall fescue seed is broadcast on the area of the circle of 8 centimetres of diameters and with upper carrier and is fixed, and makes Festuca Arundinacea and plants living band, to guarantee the homogeneity of seed; This just becomes Festuca Arundinacea and plants living its fixed form: the boiling water of putting into 100 ℃ for edible wheat flour fully stirs, and makes the paste shape, just makes the carrier fixing agent; The ratio of its wheat flour and water is 1:10.
The soil moisture severe water stress is adopted in this experiment, and soil moisture content should be the 35%-50% of saturation moisture capacity.Namely 200 g soil moisture contents should be 30.48-43.54 g in the cup, add to plant to give birth to be with weight to be about 2 g, if will keep severe water stress, then whole cup heavily is about 240 g.Guarantee that plant is in the severe water stress process, remain on when namely water every day to weigh about 240 g.After planting guarantee the seed germination water requirement until sprout.Sprout after 5 days, begin to coerce, record each and process and to water the water yield every day.Sprout after 40 days, measure the various physiological and biochemical indexs of Festuca Arundinacea seedling, and cradle aerial part.Continue to cultivate 40 days, results eldish and under ground portion, total cultivation cycle is 80 d.The laboratory average room temperature is 18-22 ℃, and medial humidity is 30-40%.
1.3 index determining method
1.3.1 the mensuration of biomass
Lawn plant is cradled, and aerial part and under ground portion are clean with distilled water flushing respectively, and thieving paper blots surface moisture, puts into baking oven, and 1 h that completes under 105 ℃ is dried to constant weight for 80 ℃, measures the dry weight of its aerial part and under ground portion, is considered as biomass.
1.3.2 the mensuration of chlorophyll content
" plant physiology and biochemistry experimental principle and the technology " that measuring chlorophyll content is edited with reference to Li Hesheng (2000): namely get 0.02 g plant leaf blade (doing) in mortar, add a small amount of silica sand and calcium carbonate and a little 80% acetone, fully grind to form pasty state, 3～5 min are left standstill in the dark place, then use Filter paper filtering, constant volume is to measure light absorption value under 633 nm and 645 nm with spectrophotometer at wavelength in 25 ml volumetric flasks, and calculates chlorophyll content according to formula.
1.3.3 the mensuration of relative conductivity
Relative conductivity adopts the conductivity meter method to measure (Tang Zhangcheng, 1999): the deionized water of 10 ml is put into numbered test tube, measure I
0Each sample takes by weighing blade 0.2 g, is cut into 0.5 cm
2Fritter place test tube, accurately add deionized water 10 mL, soak 2-4 h, be measured as I with DDS-11D direct-reading conductivity meter mensuration conductivity
1After the mensuration test tube is placed on boiling water bath 0.5 h, kill tissues is surveyed I under room temperature
2Calculate relative conductivity (%)=(I
1-I
0)/(I
2-I
0) * 100%.
1.4 data are processed
Data analysis adopts Microsoft Excel 2003 and SPSS 13.0 to carry out the independent data T check analysis of two samples.
2 development results and analysis
2.1 water stress is planted the impact of living lawn planting grass biomass on the discarded object carrier
By as seen from Table 1, during the water stress, do not lay and plant the gradually flavescence of turfgrass blade of giving birth to band, the wilting phenomenon is arranged, each discarded object is planted living planting carrier turfgrass biomass apparently higher than contrast, illustrates that water stress has obviously suppressed the growth of turfgrass.Concerning Festuca Arundinacea, the biomass of CFG, CFT, NFG and NFT has reached respectively 131.68 ﹪, 135.65 ﹪, 134.30 ﹪ and 131.89 ﹪ of contrast.Respectively plant give birth to band and contrast between significant difference (
P﹤ 0.05).
Table 1 water stress is planted the impact of living planting Festuca Arundinacea ground biomass on the discarded object carrier
2.2 water stress is planted the impact of the green cellulose content of living lawn planting blade of grass on the discarded object carrier
Water stress reduces the photosynthesis ability of plant by all means, from suppressing the stretching, extension of leaf area, to the photochemistry and the chemical-biological activities that reduce chloroplast.
As can be seen from Table 3, during the water stress, the chlorophyll content of each processed group contrasts the decline that all has in various degree.Concerning Festuca Arundinacea, the variation of chlorophyll a and chlorophyll a/b is the most obvious, except CFT, each is processed, and all there were significant differences compared with the control (
P﹤ 0.05).Explanation is under the lack of water condition, and the chlorophyll content that generates in the plant leaf blade reduces, thereby reduces photosynthesis, to guarantee the required moisture of plant normal growth.
Table 3 water stress is planted the impact of the green cellulose content of living lawn planting blade of grass on the discarded object carrier
2.4 water stress is planted the impact of living lawn planting grass relative conductivity on the discarded object carrier
Test findings shows that during water stress, the conductivity of each processed group has slightly decline compared with the control.In the Festuca Arundinacea, CFG compared with the control, difference has reached the level of signifiance, all the other each process with contrast difference little.
Table 4 water stress is on the impact of turfgrass relative conductivity
3 conclusions
Plant can produce complicated Physiology and biochemistry response under drought stress.Certain coercing in the scope, vegetable cell can initiatively change the content of some small-molecule substances and regulate born of the same parents' inner penetration gesture, makes it still can absorb moisture in the hyposmosis habitat, guarantees normal growth and the growth of plant.Though turfgrass has multiple drought resisting mechanism, every kind of mechanism all will make its body physiological process keep sufficient moisture supply, otherwise turfgrass will lack of water death.Discarded object is planted the accumulation that living band can obviously increase the turfgrass ground biomass.During water stress, each discarded object is planted living planting carrier turfgrass biomass apparently higher than contrast.Concerning Festuca Arundinacea, the biomass of CFG, CFT, NFG and NGT has reached respectively 131.68 ﹪, 135.65 ﹪, 134.30 ﹪ and 131.89 ﹪ of contrast.Respectively plant give birth to band and contrast between significant difference (
P﹤ 0.05).The chlorophyll content that generates in the plant leaf blade reduces relatively, thereby reduces photosynthesis, reduces the plant water requirement, guarantees the required moisture of plant normal growth.Concerning Festuca Arundinacea, except CFT, each chlorophyll a of processing with chlorophyll a/b contrast compare that all there were significant differences (
P﹤ 0.05).The content of each chlorophyll a, chlorophyll b and Chlorophyll in processing of rye grass also obviously reduces compared with the control, and difference all reached the level of signifiance (
P﹤ 0.05).
Each relative conductivity of processing Festuca Arundinacea also makes contrast that to a certain degree decline is arranged.Wherein the relative conductivity of CFG also has significant difference compared with the control.
In sum, during plant was subject to water stress, discarded object was planted to give birth to be with good regulating action is played in the growth of turfgrass.Tracing it to its cause, may be that discarded object is planted living band as organism, can adsorb a certain amount of moisture, thereby reduce the moisture evaporation of upper soll layer, so that the relative water content of matrix soil raises, lawn plant available moisture in growth course increases.Be reflected on the growth indexes, the plant growth that is exactly processed group is vigorous, and the wilting situation is few.Be reflected on the physical signs, the every physical signs that is exactly processed group all contrasts steadily, and fluctuation is little.
Claims (1)
1. an employing is planted and is given birth to the method that band adjusting Festuca Arundinacea water is coerced chlorophyll and conductivity, it is characterized in that being undertaken by following step:
(1) preparation of discarded object planting carrier material
Choose the consistent discarded object in source as the material of cultivating the planting carrier, comprise discarded cotton; Waste newspaper; Discarded gauze; Discarded face tissue; Each material is exposed to the sun a week in the sun, soaked 10 minutes with 1% superoxol, taking-up dries in the sun; Then with waste newspaper and discarded cotton as planting the lower floor of giving birth to the planting carrier, to discard gauze and discarded face tissue as planting the upper strata of giving birth to the planting carrier, 0.5g tall fescue seed is broadcast on the area of the circle of 8 centimetres of diameters and with upper carrier and is fixed, make Festuca Arundinacea and plant living band, to guarantee the homogeneity of seed;
(2) test method
Adopt potted plant method, in container, add the original soil after drying, then Festuca Arundinacea is planted to give birth to be with and be tiled on the original soil, after planting guarantee the seed germination water requirement until sprout, after sprouting 5 d, begin to coerce, record each processing and water the water yield every day, soil moisture content is controlled at the 35%-50% of saturation moisture capacity; Sprout after 40 days, measure the various physiological and biochemical indexs of Festuca Arundinacea seedling, and cradle aerial part, continue to cultivate 40 days, results eldish and under ground portion, total cultivation cycle is 80 days, and the laboratory average room temperature is 18-22 ℃, and medial humidity is 30-40%;
(3) index determining
Festuca Arundinacea is cradled, and aerial part and under ground portion are clean with distilled water flushing respectively, and thieving paper blots surface moisture, puts into baking oven, and 1 h that completes under 105 ℃ is dried to constant weight for 80 ℃, measure the variation that Festuca Arundinacea water is coerced chlorophyll and conductivity; Wherein the carrier fixing means refers to: edible wheat flour is put into 100 ℃ boiling water and is fully stirred, and makes the paste shape, just makes the carrier fixing agent, and is adhesively fixed; The ratio of its wheat flour and water is 1:10.
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| CN105717057A (en) * | 2016-02-01 | 2016-06-29 | 中国科学院寒区旱区环境与工程研究所 | Method for determining amino acid leakage rate of plant tissues |
| CN110687007B (en) * | 2019-11-11 | 2022-05-13 | 浙江工业大学 | Method for judging moisture content of tea leaves in fixation process of low-pressure dehumidification fixation machine |
| CN110736811A (en) * | 2019-11-19 | 2020-01-31 | 上海应用技术大学 | Petunia PhERF2 transgenic strain and waterlogging tolerance identification method of wild type thereof |
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| CN100403875C (en) * | 2005-06-27 | 2008-07-23 | 天津师范大学 | Lawn seed nursery belt sewn with household wastes and method for manufacturing the same |
| CN101869030B (en) * | 2010-06-04 | 2012-11-21 | 天津师范大学 | Domestication method adopted for enhancing salt resistance of festuca arundinacea |
| CN101884278B (en) * | 2010-06-04 | 2012-07-04 | 天津师范大学 | Method for improving activity of protective enzyme of festuca arundinacea under drought conditions |
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