CN102273337A - Application method for enhancing stress resistance of plants by biologic polysaccharide levan - Google Patents
Application method for enhancing stress resistance of plants by biologic polysaccharide levan Download PDFInfo
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- CN102273337A CN102273337A CN201110174170A CN201110174170A CN102273337A CN 102273337 A CN102273337 A CN 102273337A CN 201110174170 A CN201110174170 A CN 201110174170A CN 201110174170 A CN201110174170 A CN 201110174170A CN 102273337 A CN102273337 A CN 102273337A
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Abstract
The invention relates to an application method for enhancing the stress resistance of plants by biologic polysaccharide levan. The biologic polysaccharide levan is used as an important permeation regulating substance, and the biologic polysaccharide levan of which the concentration is 0.5%, 1%, 3% and 5% is used for soaking the plant seeds for 10-60 min or for watering the root system or spraying the leaves once or twice every day during the growth period of the seedlings for 3-30 days without intermission; the application method comprises the following detailed steps of: preparing the levan as a solution with a needed concentration by water, stirring the solution uniformly at room temperature, then using a small spraying device to spray the solution on the leaves or directly using the solution to soak the seeds or to water the roots, wherein the levan is mainly used for various crops, vegetables and fruits, such as wheat, rice, cuke, cayenne pepper and watermelon. The action mechanism of the application method disclosed by the invention is to increase the photosynthesis and to improve the metastasis, so the plants are promoted to resist the environmental stress and to have higher adaptability in adverse situation via the increment of dry matter accumulation and energy reservation, wherein the biologic polysaccharide levan causes no toxin and pollution, and causes no bad effects to the plants.
Description
Technical field
The present invention relates to the application of modern biotechnology, particularly a kind of application method that utilizes the biological polyoses levan to strengthen stress resistance of plant at agricultural production; Promptly be used to promote seed germination, improve seedling and plant development, strengthen resistance such as plant (crop seeds or seedling) is cold-resistant, drought resisting.
Background technology
Plant is subjected to the restriction of various unsuitable environmental conditions through regular meeting in sowing and growth and development process, often cause growing and the decline of output.The resistance of plant just is meant the ability of plant antagonism poor environment (as cold-resistant, drought resisting, anti-salt etc.).Mainly improve the adaptive capacity of plant at present, guarantee its growth and output, but do not only cost height like this contrary property environment by the kind of improving plant growth environment condition or seed selection strong stress resistance, year limit for length, but also may bring some secondary faces to ring.Such as; continuous development along with China's agricultural; the industrialized agriculture scale constantly enlarges; establishment planting technology is more and more universal; though adopt facility to improve the component environment condition of plant growing, side effects such as the secondary salinization that causes thus, the low light level also do not have the obvious and effective solution so far, and because the facility cultivation majority is that low temperature season carries out in the winter time; the low temperature stress at night is the life of entail dangers to plant usually, reduces production capacity.Though there have been some patents of invention can be used to improve photosynthesis of plant now, strengthen resistance, be mostly several a large amount of or trace elements and plant hormone are cooperated by a certain percentage.Because complicated component, the mechanism of action is indeterminate, and using method is not easy to operate, and result of use is often also unstable.Also have some to research and propose materials such as using the plant hormone abscisic acid and improve plant cold resistance, but its cost is to suppress plant growing, reduces yield and quality.
Therefore, researching and developing a kind of easy and simple to handle, obvious results and utilize the biological polyoses levan to strengthen the application method of stress resistance of plant, is one of this field scientific research technical staff important difficult problem of being badly in need of solving.
Summary of the invention
The objective of the invention is to overcome the weak point of using hormone or other chemical substance to strengthen stress resistance of plant effect instability in the above-mentioned prior art or reducing output, quality; a kind of application method that utilizes the biological polyoses levan to strengthen stress resistance of plant is provided; be used to protect seed and seedling, raising photosynthesis of plant ability; promote growth and development of plants, strengthen resistance.
For achieving the above object, the technical solution adopted in the present invention is as follows:
A kind of application method that utilizes the biological polyoses levan to strengthen stress resistance of plant, it is characterized in that adopting biological polyoses levan Levan, as important osmotic adjustment material, use 0.5%, 1%, 3%, 5% concentration in plant seed seed soaking 10～60 minutes, or water root system during the growth of seedling or spray the cloth blade, according to floristics growth cycle continuous application 3～30 days, every day 1～2 time; Concrete operations step: levan Levan water is mixed with 0.5%, 1%, 3%, 5% concentration solution, at room temperature stirs, use small-sized sprayer unit to spray plant leaf blade then or directly with solution seed soaking or irrigating plant root.Mainly be used on various crops such as wheat, paddy rice, cucumber, capsicum, watermelon and the vegetable melon and fruit.
The invention has the beneficial effects as follows: the used levan of the present invention is by the microbial fermentation gained, is a kind of metabolite of bacillus, and is nontoxic.The present invention uses single biological polyoses-levan (Levan), levan (Levan) is with β-2, the D-residue of fructose that the 6-glycosidic bond connects is as main chain, with some β-2, and the 1-glycosidic bond connects the levulan of forming as branch point, and multi-source is in microorganism.In growing process, levan (Levan) not only can be used as a kind of storage carbohydrate carbon source and energy is provided, but also can be used as important osmotic adjustment material, strengthen the metabolism of plant, promote pore open, improve photosynthetic rate, increase dry matter and soluble sugars, improve the drought resisting of plant, resistance such as cold-resistant.Because levan is a kind of levulan that derives from microorganism, be that microorganism is that fermentation substrate is synthetic with sucrose.Be easy to by biological degradation, noresidue, pollution-free, to the human body nonhazardous, can be applicable to the nuisanceless production of land for growing field crops and industrialized agriculture.
Use the method for enhancing stress resistance of plant of the present invention, can obviously promote plant metabolism and photosynthesis, increase adaptive capacity adverse circumstance.Because its mechanism of action is to improve photosynthesis, improve metabolism, by increasing dry-matter accumulation and energy reserve, promote plant to resist environment-stress, thereby without any side effects to plant itself, be a kind of technology of nontoxic pollution-free.
The present invention promotes plant growing simultaneously and strengthens resistance; This method is easy and simple to handle, and preparation is easy, effect stability, obvious, and applied range; The effect highly significant has vast market prospect.
Embodiment
Below in conjunction with preferred embodiment, to details are as follows according to embodiment provided by the invention:
Embodiment 1
A kind of application method that utilizes the biological polyoses levan to strengthen stress resistance of plant, this method adopts biological polyoses-levan Levan (Lay ten thousand), as important osmotic adjustment material, use 0.5%, 1%, 3%, 5% concentration in plant seed seed soaking 10～60 minutes, or water root system during the growth of seedling or spray the cloth blade, according to floristics growth cycle continuous application 3～30 days, every day 1～2 time, preferred working concentration 1%; Concrete operations step: levan (Levan) water is mixed with the solution of 0.5%, 1%, 3%, 5% mass concentration, at room temperature stirs, use small-sized sprayer unit to spray blade then or directly with solution seed soaking or pouring root; Mainly be used on various crops such as wheat, paddy rice, cucumber, capsicum, watermelon and the vegetable melon and fruit.
Embodiment 2
This method adopts biological polyoses levan (Levan), as important osmotic adjustment material, using 0.5%, 1%, 3%, 5% concentration soaks seed in plant seed, according to floristics growth cycle continuous application 3～30 days, for example be used for rice seed soaking 10～20 minutes, or other plant seed was soaked seed 10～60 minutes; Preferred working concentration 1%; Concrete operations step: levan (Levan) water is mixed with the solution of 0.5%, 1%, 3%, 5% mass concentration, at room temperature stirs, directly use solution seed soaking then; Mainly be used on various crops such as wheat, paddy rice, cucumber, capsicum, watermelon and the vegetable melon and fruit.
Other is with embodiment 1.
Embodiment 3
This method adopts biological polyoses-levan (Levan), as important osmotic adjustment material, use 0.5%, 1%, 3%, 5% concentration and during growth of seedling, water root system, according to floristics growth cycle continuous application 3～30 days, for example be used to water wheat root 7～20 days, every day 1～2 time; Preferred working concentration 1%; Concrete operations step: levan (Levan) water is mixed with the solution of 0.5%, 1%, 3%, 5% mass concentration, at room temperature stirs, then the irrigating plant root; Mainly be used on various crops such as wheat, paddy rice, cucumber, capsicum, watermelon and the vegetable melon and fruit.
Other is with embodiment 1.
Embodiment 4
This method adopts biological polyoses-levan (Levan), as important osmotic adjustment material, use 0.5%, 1%, 3%, 5% concentration in spray cloth blade, according to floristics growth cycle continuous application 3～30 days, for example be used to spray cloth cucumber or capsicum blade 5～10 days, every day 1～2 time; Preferred working concentration 1%; Concrete operations step: levan (Levan) water is mixed with the solution of 0.5%, 1%, 3%, 5% mass concentration, at room temperature stirs, use small-sized sprayer unit to spray blade then; Mainly be used on various crops such as wheat, paddy rice, cucumber, capsicum, watermelon and the vegetable melon and fruit.
Other is with embodiment 1.
Below in conjunction with detecting the effect instantiation, illustrate that levan (Levan) is to strengthening the remarkable effect of stress resistance of plant:
Detect effect example 1
(1) the wheat seed drought resistance is identified: in indoors artificial simulating drought condition, carry out wheat malt phase drought resistance and identify.
(2) at first will place 0.1% mercuric chloride solution for planting experimentally son, sterilization 10-15 minute.
(3) secondly, bottom is put into the thick absorbent cotton of 1.5CM in the culture dish of diameter 10CM, put 2 qualitative filter papers above, cotton and filter paper add the 15PEG(polyethylene glycol respectively) solution 6ml and sucrose solution is some, 0.5% Levan solution, 1% Levan solution, 3% Levan solution, 5% Levan solution, filter paper and cotton are soaked into (with pure water preparation Levan solution, every ware adds about 45ml), every ware is evenly put 20 of neat healthy seeds, repeats 3-4 time.
(4) culture dish is put into germinating box, 25 ℃ of germination 7d, during irregularly add a certain amount of Levan solution, the contrast do not add.After sprouting the 3rd day and the 7th day respectively, measure percentage of seedgermination, product are decided the drought resistance of seed.
(5) result of the test shows: use the seed of Levan solution all to compare according to the germination rate height, and growing way is also compared and shone.
Detect effect example 2
Identify the cold resistance of pepper seed: after the capsicum rudiment its seedling is incubated in the plant illumination incubator the about 160 μ mol m of plant top light intensity
-2s
-1, cultivation temperature is 25-30 ℃, illumination every day 12 hours.During 3 leaf ages, with 0.5% Levan solution, 1% Levan solution, 3% Levan solution pouring capsicum root system, cultivate after 4 days respectively, seedling is transferred to carried out low temperature treatment in 5 ℃.Again seedling is transferred in the illumination box after 6 hours in low temperature treatment, cultivated after 12 hours, can see obviously that Levan handles the difference of outward appearance growing way between plant and untreated control; The leaf photosynthesis speed of Ce Dinging shows simultaneously, the Net Photosynthetic Rate (Pn) that Levan handles all blades of plant all is higher than contrast, especially the Pn value of first blade in top is significantly higher than untreated control, illustrates that Levan handles plant and can prevent the injury of low temperature to the plant photosynthesis ability to a certain extent.After the low temperature treatment pepper seedling in incubator 27 ℃ keep after 24 hours, 30% of the not enough contrast of undressed plant leaf photosynthetic rate, and blade is the shape that here withers, and Levan preliminary treatment plant leaf Pn value has returned to the control level without low temperature treatment, illustrates that the Levan preliminary treatment helps the recovery of plant photosynthesis ability behind the low temperature.
Detect effect example 3
The watermelon seedling is incubated at the about 150mol m of top light intensity
-2s
-1In the incubator, with 0.5% Levan solution, 1% Levan spray solution blade, measure Different Leaf-position Leaf Blades soluble sugar content and plant dry weight after 25 days after the rudiment.The result shows, the Levan solution-treated has obviously improved the soluble sugar content of watermelon plant leaf, wherein 0.5% Levan solution-treated makes the soluble sugar content of top first blade improve 40.82%, and its lower blade increase rate is respectively 95.75,37.64%, 55.38%; 1% Levan solution-treated makes leaf soluble improve 105.66%, 134.21%, 35.45%, 37.34% respectively, and overall average is 78.17%.Obviously, Levan handles the increase of blade soluble sugar has substantial connection with its photosynthetic rate raising.In addition, the dry weight of measuring Levan processing plant shows that 0.5%Levan and 1%Levan handle back watermelon plant dry weight and exceed 18.39%, 29.37% than untreated control respectively, illustrate that the dry-matter accumulation amount increases.But, the plant dry substance accumulation increase is not because Levan handles the inhibition to the dark respiration effect, on the contrary, the result who measures the blade respiratory rate shows, 0.5%Levan handles the dark respiration speed that has all improved the different joints of watermelon seedling position blade in various degree with 1%Levan, all amplification reaches 42%, illustrates that Levan handles the rear blade metabolic activities and strengthens.
Detect effect example 4
The cucumber seeds germination test: use pure water, 0.5% Levan solution, 1% Levan solution, 3% Levan solution seed soaking respectively, measure germination rate and be respectively 83%, 87%, 94%, 95%, percentage of seedgermination Levan seed soaking apparently higher than untreated control.Behind seed sprouting the 7th day, measure the influence of different disposal to growth of seedling, find that Levan handles and can obviously promote the plant growth of seedling.
Detect effect example 5
With the cucumber seeds is material, and Levan is to the influence of cucumber seedling physiological property under the normal and condition of salt stress of research.Select the cucumber seeds of full seed, with after the 1% hypochlorous acid sterilization, proper alignment places the vernalization of 25 ℃ of incubator constant temperature in the culture dish that is covered with moistening filter paper, during add one time water sooner or later, to keep filter paper moistening.Selecting even seeds germinated behind the 48h transplants and grows seedlings.Pre-cultivate 15d after, add 0 respectively, the NaCl of 0.5mol/L, with small-sized sprayer unit kind of a seedling leaf, root are sprayed 0.5%, 1%, 3% Levan solution respectively simultaneously, measure behind the processing 15d.When plant was in salt stress, the destruction of at first causing was membrane damage, caused the saturating property increase of film.Therefore can be according to organizing the electrolyte volumes of extravasation result to judge the extent of injury that under the condition of salt stress plant is caused, and represent with relative conductivity.Result of the test shows that compared with the control, NaCl handles and makes the electrical conductivity of cucumber plant raise 43.2%, has significant difference; And Different L evan processing has all reduced because of NaCl handles the relative conductivity that raises, and the variable concentrations Levan processing the alleviation to a certain extent injury of salt stress to the cucumber seedling cytoplasma membrane is described.Proline is as the reflection plant senescence or suffer one of index of adverse circumstance extent of injury, and is fairly obvious to the influence of cucumber saline-alkaline tolerance under the salt stress.NaCl handles and to make that proline content is significantly higher than contrast in the cucumber leaves, and variable concentrations Levan handles all significantly to have reduced because of NaCl and handles the content that makes the proline that cucumber plant raises.
Plant can produce the osmotic adjustment effect under multiple adverse circumstance, a large amount of experiments show, under salt stress, plant cell has activity in the infiltration and the nontoxic organic matter of pair cell carries out osmotic adjustment by synthesizing, being accumulated in.By applying the Levan of variable concentrations, can reduce the content of cucumber leaves relative conductivity and proline, thereby alleviate the inhibitory action of salt stress cucumber growth.
Above-mentioned detailed description of this application method that utilizes the biological polyoses levan to strengthen stress resistance of plant being carried out with reference to embodiment; be illustrative rather than determinate; can list several embodiment according to institute's limited range; therefore in the variation and the modification that do not break away under the general plotting of the present invention, should belong within protection scope of the present invention.
Claims (1)
1. application method that utilizes the biological polyoses levan to strengthen stress resistance of plant, it is characterized in that adopting biological polyoses levan Levan, as important osmotic adjustment material, use 0.5%, 1%, 3%, 5% concentration in plant seed seed soaking 10～60 minutes, or water root system during the growth of seedling or spray the cloth blade, according to floristics growth cycle continuous application 3～30 days, every day 1～2 time; Concrete operations step: levan Levan water is mixed with 0.5%, 1%, 3%, 5% concentration solution, at room temperature stirs, use small-sized sprayer unit to spray plant leaf blade then or directly with solution seed soaking or irrigating plant root.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105493686A (en) * | 2016-02-02 | 2016-04-20 | 成都大学 | Method for increasing emergence rate of fagopyrum tataricum seeds |
| CN114271277A (en) * | 2021-12-28 | 2022-04-05 | 南京农业大学 | Application of lucuma fructan |
| CN115568321A (en) * | 2022-09-16 | 2023-01-06 | 江苏省农业科学院 | Method for prolonging physiological function period of Sucui No. 1 pear leaves |
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| KR20090033937A (en) * | 2007-10-02 | 2009-04-07 | 주식회사 리얼바이오텍 | Functional levan chungkukjang powder and the manufacturing methods |
| CN102007923A (en) * | 2010-11-19 | 2011-04-13 | 西北农林科技大学 | Laxogenin and derivative thereof-containing plant growth regulator and application thereof |
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- 2011-06-27 CN CN201110174170A patent/CN102273337A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| KR20090033937A (en) * | 2007-10-02 | 2009-04-07 | 주식회사 리얼바이오텍 | Functional levan chungkukjang powder and the manufacturing methods |
| CN102007923A (en) * | 2010-11-19 | 2011-04-13 | 西北农林科技大学 | Laxogenin and derivative thereof-containing plant growth regulator and application thereof |
Non-Patent Citations (1)
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105493686A (en) * | 2016-02-02 | 2016-04-20 | 成都大学 | Method for increasing emergence rate of fagopyrum tataricum seeds |
| CN105493686B (en) * | 2016-02-02 | 2018-03-30 | 成都大学 | A kind of method for improving bitter buckwheat seeding ratio |
| CN114271277A (en) * | 2021-12-28 | 2022-04-05 | 南京农业大学 | Application of lucuma fructan |
| CN115568321A (en) * | 2022-09-16 | 2023-01-06 | 江苏省农业科学院 | Method for prolonging physiological function period of Sucui No. 1 pear leaves |
| CN115568321B (en) * | 2022-09-16 | 2024-04-02 | 江苏省农业科学院 | Method for prolonging physiological function period of leaf of Su Cui No. 1 pear |
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Application publication date: 20111214 |