CN105493686B - A kind of method for improving bitter buckwheat seeding ratio - Google Patents
A kind of method for improving bitter buckwheat seeding ratio Download PDFInfo
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- CN105493686B CN105493686B CN201610074702.3A CN201610074702A CN105493686B CN 105493686 B CN105493686 B CN 105493686B CN 201610074702 A CN201610074702 A CN 201610074702A CN 105493686 B CN105493686 B CN 105493686B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
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Abstract
The present invention relates to agricultural technology field, discloses a kind of method for improving bitter buckwheat seeding ratio.The method of the invention extracts polysaccharide with the bacterial strain that deposit number is CGMCC No.10101, polysaccharide solution immersion bitter buckwheat seed is then configured to, then by the seed soaked in sowing season bunch planting.Raw fungi polysaccharide pre-processes to bitter buckwheat seed within the present invention, lift the ability that bitter buckwheat seed is sprouted and emerged, sowed by bunch planting technology, and then improve the emergence rate of bitter buckwheat seed, compared to existing in order to ensure the excessive type of seeding of emergence rate, on the one hand make seed density rationally uniform, beneficial to the high yield of bitter buckwheat；On the other hand it can reduce with weight savings production cost, a wide range of can be applicable.
Description
Technical field
The present invention relates to agricultural technology field, particularly relates to a kind of method for improving bitter buckwheat seeding ratio.
Background technology
Fagopyrum polygonaceae (Polygonaceae) dicotyledon, buckwheat have two cultigens, respectively sweet cherry roots
(Fagopyrum esculentum) and duck wheat (Fagopyrum tataricum).Buckwheat is main originating from China, sweet cherry roots
Be distributed in the sweet area such as peaceful in the Inner Mongol, Shan to the north of China Qinling Mountains, duck wheat be mainly distributed on China's cloud, expensive, river etc. it is high and cold and
Hills.
Bitter buckwheat scientific name hull buckwheat, alias buckwheat leaf seven, wild blue buckwheat, Wan Nianqiao, spinach wheat, black wheat, Hua Qiao.Bitter buckwheat is than sweet tea buckwheat
Nutritive value is higher by a lot, and the content of particularly bioflavonoid is 13.5 times of sweet tea buckwheat.Bitter buckwheat is medicine very few in nature
Dual-purpose crop is eaten, according to《Compendium of Materia Medica》Record：Bitter buckwheat bitter, it is cold, the real stomach of energy, benefiting energy, continue spirit, sharp knowledge, refining
The five internal organs slag is dirty；《Qianjin Yao Fang》、《Dictionary of medicinal plant》It is and all on the books to bitter buckwheat in pertinent literature：It can calm the nerves, lively atmosphere blood, drop
The wide intestines of gas, heat-clearing swell wind pain, productization of dispelling is stagnant, gut purge, ease constipation, defaecation, cough-relieving, relieving asthma, anti-inflammatory, antiallergy, cardiac stimulant, fat-reducing, U.S.
Hold and other effects.Bitter buckwheat is increasingly valued by people, market demand day in recent years due to its unique nutrition and medical value
Benefit increase.Therefore, develop duck wheat production to abundant nutrition health food, promote peasant's increasing both production and income, the characteristic production of development buckwheat
Industry has important practical significance.
However, due to bitter buckwheat seed, storage tolerance, viability do not reduce comparatively fast, and typically storing more than 2 years germination percentages is only
40-60% or so, emergence rate are then lower.To ensure certain emergence rate, excessive sowing is generally required when being sowed in production, but
It is that such one side easily causes Density inhomogeneity, is unfavorable for the high yield of bitter buckwheat；Another aspect sowing quantity is excessive, and increase is produced into
This.These have all had a strong impact on the production of bitter buckwheat.
In current agricultural planting technique, the side of duck wheat emergence rate can be also improved without a kind of simple and effective method
Method.It is therefore proposed that a kind of method for improving duck wheat emergence rate, to improving duck wheat emergence rate after planting, reduce seed into
This, increase buckwheat yield has important theory and realistic meaning.
The content of the invention
In view of this, it is an object of the invention to it is a kind of improve bitter buckwheat seeding ratio method, methods described relative to
Common bitter buckwheat implantation methods can be obviously improved its emergence rate, and then reduce application rate and cost, promote bitter buckwheat high yield.
To achieve these goals, the present invention provides following technical scheme：
A kind of method for improving bitter buckwheat seeding ratio, including：
Step 1, the bacterial strain for being CGMCC No.10101 with deposit number extraction polysaccharide
Step 2, the polysaccharide obtained with step 1 are configured to polysaccharide solution immersion bitter buckwheat seed, the kind that then will be soaked
Son is in sowing season bunch planting.
For bitter buckwheat seeding ratio it is low the problem of, the present invention use specific endogenetic fungus in advance before being planted to bitter buckwheat
Polysaccharide solution is soaked seed, and is stimulated bitter buckwheat seed to sprout with this, is lifted bitter buckwheat seed sprouting ability.Wherein, Nei Shengzhen used in the present invention
Bacterium deposit number is CGMCC No.10101, and China Committee for Culture Collection of Microorganisms is preserved on November 21st, 2014
Common micro-organisms center.
Preferably, step 1 can be specially：
Step 1.1, deposit number is removed as monose, disaccharides and the lipid material in CGMCC No.10101 bacterial strain；
Step 1.2, the bacterial strain distilled water circumfluence distillation that monose, disaccharides and lipid material will be removed, gained extract solution
Ethanol precipitation is used after concentration, after washing of precipitate, obtains bacterial strain polysaccharide.
On the basis of the method for said extracted polysaccharide, the step 1.1 can be more preferably again：
The dry mycelia that deposit number is CGMCC No.10101 is taken, is crushed, then using 95% ethanol:Petroleum ether volume ratio
For 1:1 mixed solvent carries out circumfluence distillation 2h at 5 DEG C to it, to remove the monose in mycelium, disaccharides and lipid
Material, centrifugation or filtering, collect residue, dry.
And step 1.2 can be more preferably：
It is 30mL that the bacterial strain of monose, disaccharides and lipid material, which will be removed, by ratio of water to material:1g distilled water is in 90 DEG C of heat backflows
2h is extracted, is extracted 2 times altogether；Merge No. 2 extract solutions and concentrate, add 3 times of ethanol of volume 95%, 48~72h is precipitated at 4 DEG C,
Then precipitation is collected, is washed successively with absolute ethyl alcohol, acetone, obtains bacterial strain polysaccharide.
Preferably, the concentration of the polysaccharide solution is 100-200ppm.
Preferably, the immersion is to soak 8-12h at 22-27 DEG C.
Bunch planting technology of the present invention is crop conventional seed planting technology in agricultural, this those skilled in the art be may be selected proper
When bunch planting technology sowed, but invention also provides more preferable bunch planting technology：
The disposable bottom application of composite fertilizer, later stage do not apply fertilizer before sowing, every bunch planting kind 10, depth of planting 2-3cm, line-spacing 15-
19cm, hill spacing 16-20cm, application rate 2-3kg/ mus are after planting enough to pour water, the earthing 1-2cm after soil suitably dries.Other
Same Production of Large Fields is managed, including weeds in field is manually prevented and kill off, nitrogen, P elements, the quality of potassium element in composite fertilizer used
Than for 20:7:18；The amount of application of composite fertilizer is preferably 300-420kg/hm2。
Preferably, the sowing season is annual mid-August, more preferably August 18.
Method provided by the present invention is suitable for all bitter buckwheat Wheat Seeds, and the present invention thinks western buckwheat in a specific embodiment
It is described in detail exemplified by No. 1 seed of No. 1 seed and river buckwheat, and is demonstrated by contrast test and broadcast through the method for the invention
The bitter buckwheat of kind, 15 days after planting, emergence rate was up to more than 82%, and the emergence of the bitter buckwheat seed without the processing of bacterial strain polysaccharide
Rate is 70% or so, and both are compared to having significant difference, it can thus be appreciated that the inventive method can be obviously improved bitter buckwheat seed
Emergence rate.
Beneficial effect based on the invention described above, present invention also offers the bacterial strain that deposit number is CGMCC No.10101
Application in buckwheat is planted.The buckwheat is preferably bitter buckwheat, more preferably western buckwheat 1 and river buckwheat 1.
From above technical scheme, raw fungi polysaccharide pre-processes to bitter buckwheat seed within the present invention, lifts bitter buckwheat
Seed sprouts and the ability of emergence, is sowed by bunch planting technology, and then improves the emergence rate of bitter buckwheat seed, compared to existing
In order to ensure the excessive type of seeding of emergence rate, on the one hand make seed density rationally uniform, beneficial to the high yield of bitter buckwheat；The opposing party
Face can be reduced with weight savings production cost, a wide range of can be applicable.
Biological deposits information explanation
Endophytic bacterial controlled effect Fataf6, Classification And Nomenclature are Bionectria pityrodes, are preserved on November 21st, 2014
China Committee for Culture Collection of Microorganisms's common micro-organisms center, address are Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3
Institute of Microorganism, Academia Sinica, deposit number are CGMCC No.10101.
Embodiment
The embodiment of the invention discloses a kind of method for improving bitter buckwheat seeding ratio.Those skilled in the art can use for reference
Present disclosure, it is suitably modified technological parameter realization.In particular, all similar replacements and change are to this area skill
It is it will be apparent that they are considered as being included in the present invention for art personnel.The method of the present invention is by preferably implementing
Example is described, related personnel substantially can not depart from present invention, in spirit and scope to product as described herein and
Method is modified or suitably changed with combining, to realize and using the technology of the present invention.
Experiment in specific embodiment in August, 2014 to October to Chengdu University teaching test farm (30 ° of 65 ' N,
104 ° of 19 ' E) carry out, this area height above sea level 499m, belong to subtropical zone monsoon moist climate, warm rainy, average annual rainfall is
1124.6mm, average temperature of the whole year is at 17.5 DEG C, and annual sunshine time is that experimental field soil is heavy loam to 1227h., 0~20cm
The basic nutrient situation of soil layer is：Organic matter 11.7gkg-1, full nitrogen 0.72gkg-1, full phosphorus 0.49gkg-1, full potassium
15.9g·kg-1, alkali-hydrolyzable nitrogen 51.0mgkg-1, available potassium 32.9mgkg-1, available phosphorus 23.2mgkg-1。
If not otherwise specified, all experiments of the present invention are carried out under above-mentioned environment, i.e., experimental enviroment is consistent.
In a particular embodiment, extraction method of polysaccharides used is as follows：
The dry mycelia that deposit number is CGMCC No.10101 is taken, is crushed, then using 95% ethanol:Petroleum ether volume ratio
For 1:1 mixed solvent carries out circumfluence distillation 2h at 5 DEG C to it, to remove the monose in mycelium, disaccharides and lipid
Material, centrifugation or filtering, collect residue, dry.
It is 30mL that the bacterial strain of monose, disaccharides and lipid material, which will be removed, by ratio of water to material:1g distilled water is in 90 DEG C of heat backflows
2h is extracted, is extracted 2 times altogether；Merge No. 2 extract solutions and concentrate, add 3 times of ethanol of volume 95%, 48~72h is precipitated at 4 DEG C,
Then precipitation is collected, is washed successively with absolute ethyl alcohol, acetone, obtains bacterial strain polysaccharide.
For a further understanding of the present invention, with reference to embodiment to a kind of raising bitter buckwheat seed sprouting provided by the invention
The method of rate is described in detail.
Embodiment 1：No. 1 sowing test of river buckwheat
Experiment plants bitter buckwheat kind river buckwheat 1 using southwest large area is main, by the Zhaojue agriculture of autonomous prefecture of the Sichuan Liangshan District Yi nationality, distributed over Yunnan, Sichuan and Guizhou
Industry Science Institute provides.Experiment is designed using two factor randomized block experiments, using river buckwheat 1；Using endogenetic fungus Fatf6
Mycelia water extraction polysaccharide carries out soaking induction processing to bitter buckwheat seed, then seed dries in the air naturally to 3-5 hours.Setting factor B is
Endogenetic fungus polysaccharide seed soaking concentration, B1：Do not soak seed to compare (CK)；B2：100ppm；B3：200ppm；B4：400ppm；B5：
800ppm.Plot area 7.6m is set2(2m × 3.8m), per cell 20 row, line-spacing 19cm, row long 2m, hill spacing 20cm, every row 10
Cave.Experiment uses bunch planting, and per 10, cave, 300kg/hm is pressed before sowing2Bottom application composite fertilizer (N:P:K=20:7:18) rear earthing, is broadcast
1cm.Other control measures are managed by high-yield field, pay attention to weeding and the prevention and control of plant diseases, pest control.After planting 15 days statistics emergence rates, are as a result shown in
Table 1.
Table 1
| Endogenetic fungus polysaccharide seed soaking concentration | Emergence rate (%) | 5% level of signifiance | 1% level of signifiance |
| 200ppm | 84.78 | a | A |
| 100ppm | 82.64 | a | A |
| 400ppm | 72.73 | b | B |
| 0(CK) | 71.92 | b | B |
| 800ppm | 66.61 | c | C |
By table 1, it is apparent that by bacterial strain polysaccharide 100-200ppm seed soakings seed, its emergence rate is significantly high
In blank control and the processing of other concentration, show that the emergence rate of the bitter buckwheat seed under the method for the invention is significantly carried
It is high.
Embodiment 2：Western No. 1 sowing test of buckwheat
Experiment plants the western buckwheat 1 of bitter buckwheat kind using southwest large area is main, is provided by Xichang College.Experiment using two because
Plain randomized block experiment design, using western buckwheat 1；Bitter buckwheat seed is carried out using bitter buckwheat endogenetic fungus Fat6 mycelia water extraction polysaccharide
Induction processing is soaked, then seed is dried in the air naturally to 3-5 hours.Setting factor B is endogenetic fungus polysaccharide seed soaking concentration, B1：No
Soak seed to compare (CK)；B2：100ppm；B3：200ppm；B4：400ppm；B5：800ppm.Plot area 7.2m is set2(1.6m
× 4.5m), per cell 30 row, line-spacing 15cm, row long 1.6m, hill spacing 16cm, the every cave of row 10.Experiment uses bunch planting, per 10, cave,
420kg/hm is pressed before sowing2Bottom application composite fertilizer (N:P:K=20:7:18) rear earthing 2cm, is broadcast.Other control measures press high-yield field
Management, pays attention to weeding and the prevention and control of plant diseases, pest control.After planting 15 days statistics emergence rates, the results are shown in Table 2.
Table 2
| Concentration for the treatment of | Emergence rate (%) | 5% level of signifiance | 1% level of signifiance |
| 200ppm | 86.08 | a | A |
| 100ppm | 85.38 | a | A |
| 400ppm | 74.05 | b | B |
| 0(CK) | 73.26 | b | B |
| 800ppm | 67.25 | c | C |
By table 2, it is apparent that by bacterial strain polysaccharide 100-200ppm seed soakings seed, its emergence rate is significantly high
In blank control and the processing of other concentration, show that the emergence rate of the bitter buckwheat seed under the method for the invention is significantly carried
It is high.
The explanation of above example is only intended to help the method and its core concept for understanding the present invention.It should be pointed out that pair
For those skilled in the art, under the premise without departing from the principles of the invention, the present invention can also be carried out
Some improvement and modification, these are improved and modification is also fallen into the protection domain of the claims in the present invention.
Claims (5)
- A kind of 1. method for improving bitter buckwheat seeding ratio, it is characterised in that including：Step 1, the bacterial strain for being CGMCC No.10101 with deposit number extraction polysaccharide；Step 1.1, the dry mycelia that deposit number is CGMCC No.10101 is taken, crushed, then using 95% ethanol:Petroleum ether body Product is than being 1:1 mixed solvent carries out circumfluence distillation 2h at 5 DEG C to it, so as to remove the monose in mycelium, disaccharides and Lipid material, centrifugation or filtering, collect residue, dry；Step 1.2, will to remove the bacterial strain of monose, disaccharides and lipid material by ratio of water to material be 30mL:1g distilled water is in 90 DEG C of warm Refluxing extraction 2h, extract 2 times altogether；Merge No. 2 extract solutions simultaneously concentrate, add 3 times of ethanol of volume 95%, at 4 DEG C precipitate 48~ 72h, precipitation is then collected, is washed successively with absolute ethyl alcohol, acetone, obtain bacterial strain polysaccharide；Step 2, the polysaccharide obtained with step 1 are configured to the polysaccharide solution immersion bitter buckwheat seed that concentration is 100-200ppm, so Afterwards by the seed soaked in sowing season bunch planting.
- 2. method according to claim 1, it is characterised in that the immersion is to soak 8-12h at 22-27 DEG C.
- 3. method according to claim 1, it is characterised in that the bunch planting is specially：The disposable bottom application of composite fertilizer, later stage do not apply fertilizer before sowing, per bunch planting kind 10, depth of planting 2-3cm, line-spacing 15-19cm, Hill spacing 16-20cm, application rate 2-3kg/ mus are after planting enough to pour water, the earthing 1-2cm after soil suitably dries.
- 4. method according to claim 1, it is characterised in that the bitter buckwheat seed is western No. 1 seed of buckwheat or No. a kind of river buckwheat Son.
- 5. method according to claim 1, it is characterised in that the sowing season is annual mid-August.
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| CN107494541B (en) * | 2017-08-28 | 2020-09-11 | 成都大学 | Lodging-resistant regulator and method for improving lodging resistance of crops in seedling stage |
| CN109429946A (en) * | 2018-12-18 | 2019-03-08 | 叶发安 | The implantation methods of bitter buckwheat |
| CN109644796B (en) * | 2019-02-26 | 2021-07-13 | 西南大学 | Cultivation method for mechanically sowing and improving lodging resistance of tartary buckwheat |
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