CN109429946A - The implantation methods of bitter buckwheat - Google Patents
The implantation methods of bitter buckwheat Download PDFInfo
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- CN109429946A CN109429946A CN201811554404.XA CN201811554404A CN109429946A CN 109429946 A CN109429946 A CN 109429946A CN 201811554404 A CN201811554404 A CN 201811554404A CN 109429946 A CN109429946 A CN 109429946A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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- A01B79/02—Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
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- A01N65/08—Magnoliopsida [dicotyledons]
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Abstract
The invention discloses a kind of implantation methods of bitter buckwheat, the following steps are included: S1, selection plantation soil, soil is loosened the soil, the soil dug that will loosen the soil is uniformly mixed with charcoal, bamboo fibre, sulphur according to mass ratio 30:2:1:0.5, the hypochlorite solution that then spray concentration is 5%, after mixing evenly, by 350kg/ mus of addition base manure, after leveling, plant hole is dug, the line-spacing and spacing in the rows of plant hole are 20cm;S2, bitter buckwheat seed is taken, places it in and impregnates 10~15min in the soak that temperature is 20~25 DEG C, the bitter buckwheat seed after immersion is sowed into the depth of planting 3cm in plant hole.The implantation methods of bitter buckwheat of the invention more than the emergence rate that bitter buckwheat seed can be improved, and can promote the growth of Miao Hegen.
Description
Technical field
The present invention relates to field of agricultural cultivation.It is more particularly related to a kind of implantation methods of bitter buckwheat.
Background technique
Bitter buckwheat scientific name hull buckwheat, alias buckwheat leaf seven, wild blue buckwheat, Wan Nianqiao, spinach wheat, black wheat, Hua Qiao.Bitter buckwheat is than sweet tea buckwheat
Nutritive value is higher by very much.Bitter buckwheat is dual-purpose of drug and food crop very few in nature, is recorded according to Compendium of Material Medica: bitter buckwheat bitter,
Cold, the real stomach of energy, benefiting energy continues spirit, and it is dirty to refine the five internal organs slag for sharp knowledge.Bitter buckwheat is due to its unique nutrition and medicinal valence
Value, is increasingly valued by people, market demand increasingly increases in recent years.Therefore, develop tartary buckwheat production to abundant nutrition
Health food promotes peasant's increasing both production and income, develops bitter buckwheat specialty industries and has important practical significance.However, due to bitter buckwheat kind
Storage tolerance, viability do not reduce comparatively fast son, and emergence rate is then lower.To guarantee certain emergence rate, often needed when being sowed in production
It excessively to sow, kind amount is excessive, increases production cost.These have all seriously affected the production of bitter buckwheat.Therefore, being badly in need of one kind can mention
The implantation methods of high bitter buckwheat emergence rate.
Summary of the invention
It is an object of the invention to solve the above problems, and provide the advantages of at least will be described later.
It is a still further object of the present invention to provide a kind of implantation methods of bitter buckwheat, can be improved the emergence rate of bitter buckwheat seed with
On, and can promote the growth of Miao Hegen.
In order to realize these purposes and other advantages according to the present invention, a kind of implantation methods of bitter buckwheat are provided, including
Following steps:
S1, selection plantation soil, soil is loosened the soil, will the loosen the soil soil dug and charcoal, bamboo fibre, sulphur by
It is uniformly mixed according to mass ratio 30:2:1:0.5, the hypochlorite solution that then spray concentration is 5%, after mixing evenly, by 350kg/
Base manure is added in mu, after leveling, digs plant hole, the line-spacing and spacing in the rows of plant hole are 20cm;
S2, bitter buckwheat seed is taken, places it in and impregnate 10~15min in the soak that temperature is 20~25 DEG C, after immersion
Bitter buckwheat seed sow in plant hole, depth of planting 3cm;
Wherein, the soak preparation method the following steps are included:
A1, by parts by weight be 5~10 parts of salvia chinensis, 3~5 parts of caulis akebiae, 1~3 part of Herba Andrographitis, 2~5 parts pale reddish brownly
Fourth, 5~8 parts of Sculellaria barbata, 1~3 part of Fructus Forsythiae, 2~5 parts of root of kirilow rhodiola, 1~5 part of Asiatic plantain after mixing, crush,
Be placed in container, adding parts by weight is 120 parts of water, impregnate 5~10h, then heating decoct until water content be 20~
30%, obtain lotion;
A2, by parts by weight be 5~10 parts of luffa pericarp, 5~10 parts of peanut, 1~3 part of sword bean, 1~2 part of Chinese yam,
1~3 part of leek after mixing, crushes, and addition parts by weight are 3 parts of Bacillus subtillis, 2 parts of lactobacillus plantarum, 1 part
Bifidobacterium lactis, at 25~30 DEG C be sealed by fermentation 5~10d, sterilize to obtain fermentation liquid;
A3, by lotion and fermentation liquid after mixing, addition parts by weight are 3 parts of cellulase, 1 part of pectase, in
Temperature is that 20~25h is digested at 35~40 DEG C, then, the ethyl alcohol that 100 parts of mass fractions are 95% is added, stirring, standing 12~
After for 24 hours, filtering obtains the first filtrate, and the first filtrate is diluted 10 times and obtains soak.
Preferably, the implantation methods of the bitter buckwheat, the base manure the preparation method comprises the following steps:
B1, by parts by weight be 5~10 parts of common cattail, 1~3 part of cloves, 1~3 part of field thistle, 5~8 parts of hawthorn, 4~
6 parts of purslane, 1~3 part of dark plum, 4~8 parts of bitter orange flower, 2~4 parts of radix polygonati officinalis, 1~5 part of the root of Dahurain angelica, the hundred of 2~6 parts
It closes, after 3~8 parts of emblic co-grinding, the water that parts by weight are 200~250 parts is added, be to be heated at 90~95 DEG C in temperature
8~12h, filtering, obtains the second filtrate and filter residue;
B2, by parts by weight be 20~30 parts of fish maw intestines, 25~35 parts of pig bone, 5~10 parts of fish bony spur, 10~15
The fish scale, 10~15 parts of chicken bone co-grinding of part, are added 2.5 parts and 3 parts of pepsin of trypsase, digest 6~10h
It is 5 parts of egg shell, 3 parts of mushroom and filter residue that parts by weight are added afterwards, and after mixing evenly, anaerobism is sent out at a temperature of 28~32 DEG C
10~15d of ferment, it is 5~10 parts of soybean, 10~15 parts of jujube, 5~10 parts of castor-oil plant, 5~10 parts that parts by weight, which are then added,
Watermelon peel, 3~6 parts of Chinese leaf, 5~10 dregs of beans, after mixing evenly, at a temperature of 20~25 DEG C anaerobic fermentation 10~
15d adds the second filtrate in B1, and the 5~10d that ferments at a temperature of 20~25 DEG C is to get base manure.
Preferably, the implantation methods of the bitter buckwheat, the bitter buckwheat seed are placed in impregnate in soak and also be placed in front of
Impregnate 2~4min in seed-soaking liquid, the seed-soaking liquid the preparation method comprises the following steps: being that weight is added in 100~150 parts of water to parts by weight
Part be 20~25 parts of bamboo vinegar and 10~15 parts of plant ash, stir evenly to get.
Preferably, the implantation methods of the bitter buckwheat are also passed through ozone, so that water in A1 while water into container
Middle ozone content is not less than 0.3mg/L.
Preferably, the implantation methods of the bitter buckwheat, the temperature digested in B2 are 37 DEG C.
Preferably, luffa pericarp, peanut, sword bean, Chinese yam, leek are crushed in A2 by the implantation methods of the bitter buckwheat
Grain diameter is that 10~20min is stirred under 800r/min less than 20 mesh, and in stirring rate.
The present invention is include at least the following beneficial effects:
1, the implantation methods of bitter buckwheat of the invention, loosen the soil to soil, and will the loosen the soil soil dug and charcoal, bamboo
Fiber, sulphur mixing, charcoal, bamboo fibre have porosity, and loosing soil is porous to be convenient for retain water and nutrients, and the bamboo being added is fine
Dimension, sulphur, hypochlorite solution can also carry out sterilizing to soil, while sulphur, hypochlorous acid can also maintain the faintly acid of soil,
In favor of the growth of bitter buckwheat.
2, the implantation methods of bitter buckwheat of the invention, will be by lotion and fermentation liquid after mixing, and it is 3 parts that parts by weight, which are added,
Cellulase, 1 part of pectase digest 20~25h, then, ethyl alcohol are added, stir, filtering obtains the first filtrate, by first
Filtrate dilutes 10 times and obtains soak, and the emergence rate which can promote bitter buckwheat seed is up to 85% or more, and can promote
Into the growth of Miao Hegen.
3, the implantation methods of bitter buckwheat of the invention, also application base manure, by fish maw intestines, pig bone, fish bony spur, fish scale, chicken
Bone first uses pepsin, trypsin digestion, then ferments, and the P elements rich in such as fish maw intestines, pig bone can be
Bitter buckwheat growth improves P elements, and generation nitrogen abundant is first after soybean, jujube, castor-oil plant, watermelon peel, Chinese leaf, dregs of beans are fermented
Element, each nutritional ingredient of base manure is balanced, is greatly improved the growth of bitter buckwheat, and experiment shows to apply the base manure height of seedling reachable
10.23cm, root long are up to 4.87cm, and it is only 1.28cm that apply conventional farmyard manure height of seedling, which be only 6.32cm, root long,.
Further advantage, target and feature of the invention will be partially reflected by the following instructions, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Specific embodiment
The present invention will be further described in detail below with reference to the embodiments, to enable those skilled in the art referring to specification
Text can be implemented accordingly.
Embodiment 1
A kind of implantation methods of bitter buckwheat, comprising the following steps:
S1, selection plantation soil, soil is loosened the soil, will the loosen the soil soil dug and charcoal, bamboo fibre, sulphur by
It is uniformly mixed according to mass ratio 30:2:1:0.5, the hypochlorite solution that then spray concentration is 5%, after mixing evenly, by 350kg/
Base manure is added in mu, after leveling, digs plant hole, the line-spacing and spacing in the rows of plant hole are 20cm;
S2, bitter buckwheat seed is taken, places it in and impregnate 10~15min in the soak that temperature is 20~25 DEG C, after immersion
Bitter buckwheat seed sow in plant hole, depth of planting 3cm;
Wherein, the soak preparation method the following steps are included:
A1, by parts by weight be 5 parts of salvia chinensis, 3 parts of caulis akebiae, 1 part of Herba Andrographitis, 2 parts of viola mandshurica, the half of 5 parts
Lotus, 1 part of Fructus Forsythiae, 2 parts of root of kirilow rhodiola, 1 part of Asiatic plantain after mixing, crush, are placed in container, adding parts by weight is
120 parts of water, impregnate 5h, then heating decoct until water content be 20%, obtain lotion;
It A2, is 5 parts of luffa pericarp, 5 parts of peanut, 1 part of sword bean, 1 part of Chinese yam, the mixing of 1 part of leek by parts by weight
Uniformly after, crush, additions parts by weight be 3 parts of Bacillus subtillis, 2 parts of lactobacillus plantarum, 1 part of bifidobacterium lactis, in
It is sealed by fermentation 5d at 25 DEG C, sterilize to obtain fermentation liquid;
A3, by lotion and fermentation liquid after mixing, addition parts by weight are 3 parts of cellulase, 1 part of pectase, in
Temperature is to digest 20h at 35 DEG C, then, the ethyl alcohol that 100 parts of mass fractions are 95% is added, stirring, after standing 12h, filtering is obtained
To the first filtrate, the first filtrate is diluted 10 times and obtains soak.
The implantation methods of the bitter buckwheat, the base manure the preparation method comprises the following steps:
B1, by parts by weight be 5 parts of common cattail, 1 part of cloves, 1 part of field thistle, 5 parts of hawthorn, 4 parts of purslane, 1 part
Dark plum, 4 parts of bitter orange flower, 2 parts of radix polygonati officinalis, 1 part of the root of Dahurain angelica, 2 parts of lily, after 3 parts of emblic co-grinding, weight is added
The water that part is 200 parts is measured, is to heat 8h at 90 DEG C in temperature, filters, obtain the second filtrate and filter residue;
It B2, is 20 parts of fish maw intestines, 25 parts of pig bone, 5 parts of fish bony spur, 10 parts of fish scale, 10 parts by parts by weight
2.5 parts and 3 parts of pepsin of trypsase is added in chicken bone co-grinding, and the egg that parts by weight are 5 parts is added after digesting 6h
Shell, 3 parts of mushroom and filter residue, after mixing evenly, the anaerobic fermentation 10d at a temperature of 28 DEG C, it is 5 parts that parts by weight, which are then added,
Soybean, 10 parts of jujube, 5 parts of castor-oil plant, 5 parts of watermelon peel, 3 parts of Chinese leaf, 5 dregs of beans, after mixing evenly, then at 20 DEG C
At a temperature of anaerobic fermentation 10d, add the second filtrate in B1, at a temperature of 20 DEG C ferment 5d to get base manure.
The implantation methods of the bitter buckwheat, the bitter buckwheat seed, which is placed in soak to impregnate also to be placed in seed-soaking liquid before, to be soaked
Steep 2min, the seed-soaking liquid the preparation method comprises the following steps: in the water for being 100 parts to parts by weight be added parts by weight be 20 parts bamboo vinegar with
And 10 parts of plant ash, stir evenly to get.
The implantation methods of the bitter buckwheat are also passed through ozone, so that ozone in water content in A1 while water into container
Not less than 0.3mg/L.
The implantation methods of the bitter buckwheat, the temperature digested in B2 are 37 DEG C.
The implantation methods of the bitter buckwheat, in A2 by luffa pericarp, peanut, sword bean, Chinese yam, that leek is crushed to grain diameter is small
It is that 10~20min is stirred under 800r/min in 20 mesh, and in stirring rate.
Embodiment 2
A kind of implantation methods of bitter buckwheat, comprising the following steps:
S1, selection plantation soil, soil is loosened the soil, will the loosen the soil soil dug and charcoal, bamboo fibre, sulphur by
It is uniformly mixed according to mass ratio 30:2:1:0.5, the hypochlorite solution that then spray concentration is 5%, after mixing evenly, by 350kg/
Base manure is added in mu, after leveling, digs plant hole, the line-spacing and spacing in the rows of plant hole are 20cm;
S2, bitter buckwheat seed is taken, places it in and impregnate 10~15min in the soak that temperature is 20~25 DEG C, after immersion
Bitter buckwheat seed sow in plant hole, depth of planting 3cm;
Wherein, the soak preparation method the following steps are included:
A1, by parts by weight be 10 parts of salvia chinensis, 5 parts of caulis akebiae, 3 parts of Herba Andrographitis, 5 parts of viola mandshurica, the half of 8 parts
Lotus, 3 parts of Fructus Forsythiae, 5 parts of root of kirilow rhodiola, 5 parts of Asiatic plantain after mixing, crush, are placed in container, adding parts by weight is
120 parts of water, impregnate 10h, then heating decoct until water content be 30%, obtain lotion;
It A2, is that 10 parts of luffa pericarp, 10 parts of peanut, 3 parts of sword bean, 2 parts of Chinese yam, 3 parts of leek mix by parts by weight
Close uniformly after, crush, additions parts by weight be 3 parts of Bacillus subtillis, 2 parts of lactobacillus plantarum, 1 part of bifidobacterium lactis,
It is sealed by fermentation 10d at 30 DEG C, sterilize to obtain fermentation liquid;
A3, by lotion and fermentation liquid after mixing, addition parts by weight are 3 parts of cellulase, 1 part of pectase, in
Temperature is to digest 25h at 40 DEG C, then, the ethyl alcohol that 100 parts of mass fractions are 95% is added, stirring, after standing for 24 hours, filtering is obtained
To the first filtrate, the first filtrate is diluted 10 times and obtains soak.
Preferably, the implantation methods of the bitter buckwheat, the base manure the preparation method comprises the following steps:
B1, by parts by weight be 10 parts of common cattail, 3 parts of cloves, 3 parts of field thistle, 8 parts of hawthorn, 6 parts of purslane, 3 parts
Dark plum, 8 parts of bitter orange flower, 4 parts of radix polygonati officinalis, 5 parts of the root of Dahurain angelica, 6 parts of lily, after 8 parts of emblic co-grinding, weight is added
The water that part is 250 parts is measured, is to heat 12h at 95 DEG C in temperature, filters, obtain the second filtrate and filter residue;
It B2, is 30 parts of fish maw intestines, 35 parts of pig bone, 10 parts of fish bony spur, 15 parts of fish scale, 15 parts by parts by weight
2.5 parts and 3 parts of pepsin of trypsase is added in chicken bone co-grinding, and the chicken that parts by weight are 5 parts is added after digesting 10h
Eggshell, 3 parts of mushroom and filter residue, after mixing evenly, the anaerobic fermentation 15d at a temperature of 32 DEG C, it is 10 that parts by weight, which are then added,
Part soybean, 15 parts of jujube, 10 parts of castor-oil plant, 10 parts of watermelon peel, 6 parts of Chinese leaf, 10 dregs of beans, after mixing evenly,
The anaerobic fermentation 15d at a temperature of 25 DEG C adds the second filtrate in B1, and the 10d that ferments at a temperature of 25 DEG C is to get base manure.
The implantation methods of the bitter buckwheat, the bitter buckwheat seed, which is placed in soak to impregnate also to be placed in seed-soaking liquid before, to be soaked
Steep 4min, the seed-soaking liquid the preparation method comprises the following steps: in the water for being 150 parts to parts by weight be added parts by weight be 25 parts bamboo vinegar with
And 15 parts of plant ash, stir evenly to get.
The implantation methods of the bitter buckwheat are also passed through ozone, so that ozone in water content in A1 while water into container
Not less than 0.3mg/L.
The implantation methods of the bitter buckwheat, the temperature digested in B2 are 37 DEG C.
The implantation methods of the bitter buckwheat, in A2 by luffa pericarp, peanut, sword bean, Chinese yam, that leek is crushed to grain diameter is small
It is that 10~20min is stirred under 800r/min in 20 mesh, and in stirring rate.
Comparative example 1
With embodiment 1, difference is, bitter buckwheat seed is impregnated merely through seed-soaking liquid, impregnates without soak.
Comparative example 2
With embodiment 1, difference is, the base manure of application is conventional farmyard manure.
Bitter buckwheat is planted according to the method for embodiment 1 and comparative example 1~2 respectively, sowing line number is 10 rows, every row 10
Plant hole, each plant hole sow 10 bitter buckwheat seeds, count bitter buckwheat emergence rate and average height of seedling, root long after 15d, experimental result is such as
Shown in table 1.
The influence that table 1- difference embodiment grows bitter buckwheat
Emergence rate (%) | Height of seedling (cm) | Root long (cm) | |
Embodiment 1 | 86.8 | 10.23 | 4.87 |
Comparative example 1 | 73.1 | 8.36 | 2.76 |
Comparative example 2 | 81.5 | 6.32 | 1.28 |
As shown in Table 1, the emergence rate that soak of the invention can promote bitter buckwheat seed is up to 85% or more, and can promote
The growth of seedling and root.Base manure of the invention is greatly improved the growth of bitter buckwheat, applies the base manure height of seedling up to 10.23cm, root long
Up to 4.87cm, and it is only 1.28cm that apply conventional farmyard manure height of seedling, which be only 6.32cm, root long,.
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously unlimited
In specific details and embodiment shown and described herein.
Claims (6)
1. the implantation methods of bitter buckwheat, which comprises the following steps:
S1, selection plantation soil, soil is loosened the soil, and the soil dug that will loosen the soil is with charcoal, bamboo fibre, sulphur according to matter
Amount is uniformly mixed than 30:2:1:0.5, the hypochlorite solution that then spray concentration is 5%, after mixing evenly, is added by 350kg/ mus
Enter base manure, after leveling, digs plant hole, the line-spacing and spacing in the rows of plant hole are 20cm;
S2, bitter buckwheat seed is taken, places it in and impregnate 10~15min in the soak that temperature is 20~25 DEG C, by the hardship after immersion
Buckwheat seed sows the depth of planting 3cm in plant hole;
Wherein, the soak preparation method the following steps are included:
A1, by parts by weight be 5~10 parts of salvia chinensis, 3~5 parts of caulis akebiae, 1~3 part of Herba Andrographitis, 2~5 parts of viola mandshurica, 5
~8 parts of Sculellaria barbata, 1~3 part of Fructus Forsythiae, 2~5 parts of root of kirilow rhodiola, 1~5 part of Asiatic plantain after mixing, crush, are placed in
In container, adding parts by weight is 120 parts of water, impregnates 5~10h, and then heating decocts until water content is 20~30%, is obtained
Lotion;
A2, by parts by weight be 5~10 parts of luffa pericarp, 5~10 parts of peanut, 1~3 part of sword bean, 1~2 part of Chinese yam, 1~3
The leek of part after mixing, crushes, and addition parts by weight are 3 parts of Bacillus subtillis, 2 parts of lactobacillus plantarum, 1 part of cream
Bifidobacterium is sealed by fermentation 5~10d at 25~30 DEG C, and sterilize to obtain fermentation liquid;
A3, by lotion and fermentation liquid after mixing, addition parts by weight are 3 parts of cellulase, 1 part of pectase, Yu Wendu
It is 20~25h of enzymatic hydrolysis at 35~40 DEG C, then, the ethyl alcohol that 100 parts of mass fractions are 95% is added, stirring, standing 12~for 24 hours
Afterwards, it filters, obtains the first filtrate, the first filtrate is diluted 10 times and obtains soak.
2. the implantation methods of bitter buckwheat as described in claim 1, which is characterized in that the base manure the preparation method comprises the following steps:
B1, by parts by weight be 5~10 parts of common cattail, 1~3 part of cloves, 1~3 part of field thistle, 5~8 parts of hawthorn, 4~6 parts
Purslane, 1~3 part of dark plum, 4~8 parts of bitter orange flower, 2~4 parts of radix polygonati officinalis, 1~5 part of the root of Dahurain angelica, 2~6 parts of lily, 3
After~8 parts of emblic co-grinding, it is 200~250 parts of water that parts by weight, which are added, in temperature be heating 8 at 90~95 DEG C~
12h, filtering, obtains the second filtrate and filter residue;
It B2, is 20~30 parts of fish maw intestines, 25~35 parts of pig bone, 5~10 parts of fish bony spur, 10~15 parts by parts by weight
2.5 parts and 3 parts of pepsin of trypsase is added in fish scale, 10~15 parts of chicken bone co-grinding, adds after digesting 6~10h
Entering parts by weight is 5 parts of egg shell, 3 parts of mushroom and filter residue, after mixing evenly, the anaerobic fermentation 10 at a temperature of 28~32 DEG C
~15d, it is 5~10 parts of soybean, 10~15 parts of jujube, 5~10 parts of castor-oil plant, 5~10 parts of west that parts by weight, which are then added,
Melon skin, 3~6 parts of Chinese leaf, 5~10 dregs of beans, after mixing evenly, 10~15d of anaerobic fermentation at a temperature of 20~25 DEG C,
The second filtrate in B1 is added, the 5~10d that ferments at a temperature of 20~25 DEG C is to get base manure.
3. the implantation methods of bitter buckwheat as described in claim 1, which is characterized in that the bitter buckwheat seed, which is placed in soak, to be impregnated
Also be placed in seed-soaking liquid 2~4min of immersion before, the seed-soaking liquid the preparation method comprises the following steps: the water for being 100~150 parts to parts by weight
Middle addition parts by weight be 20~25 parts of bamboo vinegar and 10~15 parts of plant ash, stir evenly to get.
4. the implantation methods of bitter buckwheat as described in claim 1, which is characterized in that in A1 while water, be also passed through into container
Ozone, so that ozone in water content is not less than 0.3mg/L.
5. the implantation methods of bitter buckwheat as claimed in claim 2, which is characterized in that the temperature digested in B2 is 37 DEG C.
6. the implantation methods of bitter buckwheat as described in claim 1, which is characterized in that in A2 by luffa pericarp, peanut, sword bean, Chinese yam,
Leek is crushed to grain diameter less than 20 mesh, and is that 10~20min is stirred under 800r/min in stirring rate.
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