CN102523786B - Preparation method for novel tobacco seed germination bed - Google Patents
Preparation method for novel tobacco seed germination bed Download PDFInfo
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- CN102523786B CN102523786B CN2011104445161A CN201110444516A CN102523786B CN 102523786 B CN102523786 B CN 102523786B CN 2011104445161 A CN2011104445161 A CN 2011104445161A CN 201110444516 A CN201110444516 A CN 201110444516A CN 102523786 B CN102523786 B CN 102523786B
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- 238000002360 preparation method Methods 0.000 title claims abstract description 20
- 230000007226 seed germination Effects 0.000 title claims abstract description 16
- 241000208125 Nicotiana Species 0.000 title claims abstract description 15
- 235000002637 Nicotiana tabacum Nutrition 0.000 title claims abstract description 15
- 230000035784 germination Effects 0.000 claims abstract description 37
- 239000011347 resin Substances 0.000 claims abstract description 16
- 229920005989 resin Polymers 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 239000000843 powder Substances 0.000 claims abstract description 8
- DZSVIVLGBJKQAP-UHFFFAOYSA-N 1-(2-methyl-5-propan-2-ylcyclohex-2-en-1-yl)propan-1-one Chemical compound CCC(=O)C1CC(C(C)C)CC=C1C DZSVIVLGBJKQAP-UHFFFAOYSA-N 0.000 claims description 16
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- 238000001035 drying Methods 0.000 claims description 5
- 239000008367 deionised water Substances 0.000 claims description 4
- 229910021641 deionized water Inorganic materials 0.000 claims description 4
- 229920003023 plastic Polymers 0.000 claims description 4
- 239000004033 plastic Substances 0.000 claims description 4
- 239000003643 water by type Substances 0.000 claims description 4
- 239000002253 acid Substances 0.000 claims description 3
- 150000007513 acids Chemical class 0.000 claims description 3
- FIKFOOMAUXPBJM-UHFFFAOYSA-N hepta-2,5-dienediamide Chemical class NC(=O)C=CCC=CC(N)=O FIKFOOMAUXPBJM-UHFFFAOYSA-N 0.000 claims description 3
- USHAGKDGDHPEEY-UHFFFAOYSA-L potassium persulfate Chemical compound [K+].[K+].[O-]S(=O)(=O)OOS([O-])(=O)=O USHAGKDGDHPEEY-UHFFFAOYSA-L 0.000 claims description 3
- 235000019394 potassium persulphate Nutrition 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 10
- 239000000017 hydrogel Substances 0.000 abstract description 7
- 238000010521 absorption reaction Methods 0.000 abstract 1
- -1 poly(2-acryloylamino-2-methylpropanesulfonic acid) Polymers 0.000 abstract 1
- 239000013589 supplement Substances 0.000 abstract 1
- 235000003255 Carthamus tinctorius Nutrition 0.000 description 11
- 244000020518 Carthamus tinctorius Species 0.000 description 11
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 11
- 239000010931 gold Substances 0.000 description 11
- 229910052737 gold Inorganic materials 0.000 description 11
- 238000002474 experimental method Methods 0.000 description 4
- 230000003203 everyday effect Effects 0.000 description 3
- 239000011162 core material Substances 0.000 description 2
- 239000012297 crystallization seed Substances 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000029052 metamorphosis Effects 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000012271 agricultural production Methods 0.000 description 1
- 230000009418 agronomic effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Landscapes
- Pretreatment Of Seeds And Plants (AREA)
- Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
Abstract
The invention provides a preparation method for a novel tobacco seed germination bed. According to the method, high water absorption resin poly(2-acryloylamino-2-methylpropanesulfonic acid) (PAMPS) powder absorbs water in a culture dish to prepare the novel tobacco seed germination bed. The novel tobacco seed germination bed prepared by the method of the present invention has the following characteristics that: the bed shows the hydrogel state; the tobacco seeds can be directly placed on the gelatinous germination bed, the seeds directly absorb the water from the hydrogel during the whole germination process, such that the regular water supplement is not required; the germination bed of the present invention has good water retention property, such that the moisture does not easily volatile, and the uniform germination condition can be provided.
Description
Technical field
The present invention relates to the agronomic crop seed germination detection technique, be specifically related to a kind of can reusing and the preparation method of the novel tobacco seed germination bed that whole process need not be watered.
Background technology
Germination test is to measure the maximum germination potentiality of seed lot under the specific environmental condition of laboratory.Quality that accordingly can more different seed lots.Also can estimate field sowing is worth.Seed management department carries out Seed germination, can in time hit the production and operation behavior of fake and inferior seed.Significant to guaranteeing agricultural production security, also play an important role to regulating the seed quality dispute.
Seed germination experiment needs specific germinating bed, and at present, paper bed (germination paper, filter paper etc.) is to use a maximum class germinating bed (a spring Khanh Hoa Wang Jianhua, 2005) in seed germination experiment.Problem is that the water retention capacity of paper bed is poor, needs regular replenishment moisture.Simultaneously, the paper bed need be contained in the containers such as culture dish or germination box,, because the sealing of container is different, causes the speed of moisture evaporation in different paper beds different, is difficult to guarantee the uniformity of all germinating beds aspect water content.In addition, the paper bed will abandon after using once, can not reuse.
Summary of the invention
The object of the invention is to avoid above-mentioned the deficiencies in the prior art, provide a kind of and can reuse and the preparation method of the novel tobacco seed germination bed that whole process need not be watered.The germinating bed for preparing is the water-setting glue, preparation after being absorbed water in culture dish by poly-2-acrylamide-2-methylpro panesulfonic acid (PAMPS) powder of high hydroscopic resin.Seed can directly be placed on gelatinous germinating bed, and whole germination process seed directly absorbs water from hydrogel, therefore, does not need regular replenishment moisture.In addition, poly-2-acrylamide-2-methylpro panesulfonic acid has good water retention property, and moisture is not volatile, and more consistent germination condition can be provided.
A kind of preparation method of novel tobacco seed germination bed comprises the following steps:
1): N
2Add successively 100 g deionized waters, 35 g 2-acrylamide-2-methylpro panesulfonic acids, 12 g sodium hydroxide, 0.014 g potassium peroxydisulfate and 0.105 g N at 500 ml round-bottomed flasks under protection; N '-60 ℃ of methylene-bisacrylamides heat temperature raising is answered 12 h; 60 ℃ of drying 48 h of product, pulverize and sieve (100 order) obtain approximately 45 g of poly-2-acrylamide-2-methylpro panesulfonic acid powder.
2): a certain amount of poly-2-acrylamide-2-methylpro panesulfonic acid powder is dissolved in deionized water, stirs, the solution concentration that obtains is 0.1%;
3): the solution of 10～100mL step 2 preparation is poured in the germination box or culture dish of different size, and standing 24 h obtain gelatinous high hydroscopic resin germinating bed.
Wherein, described germination box comprises the plastics germination box of 12 * 12cm and 24 * 12cm, and culture dish comprises glass or the plastic culture dish of diameter 6cm～18cm.
Wherein, if give thousand kernel weight when the minimum grain Tobacco Seed Germination of 0.05-0.5g, the thickness of bud bed is 0.2 ~ 0.5cm.
The thickness of setting the bud bed is the growing state for the ease of the root of observing the rear seed that germinates.
Have following advantage with respect to prior art the present invention:
1): the poly-2-acrylamide-2-methylpro panesulfonic acid of the core material that the present invention uses uses the raw material preparation of relative low price, the preparation method is simple and easy, poly-2-acrylamide-2-methylpro panesulfonic acid hydrogel after use can absorb water after drying again, Reusability.
2): poly-2-acrylamide-2-methylpro panesulfonic acid hydrogel is transparence, can clearly observe the metamorphosis of root in process of growth.
3): poly-2-acrylamide-2-methylpro panesulfonic acid has good retaining water retaining function, and therefore, whole germination process does not need regular replenishment moisture, can save a large amount of manpowers.
Embodiment
In following embodiment, the seed of tobacco bred " the large gold dollar of safflower " and " MS K326 " is from China Tobacco Seed Yuxi Co., Ltd..
Embodiment 1The preparation of high hydroscopic resin germinating bed
N
2Add successively 100 g deionized waters, 35 g 2-acrylamide-2-methylpro panesulfonic acids, 12 g sodium hydroxide, 0.014 g potassium peroxydisulfate and 0.105 g N at 500 ml round-bottomed flasks under protection; N '-60 ℃ of methylene-bisacrylamides heat temperature raising is answered 12 h; 60 ℃ of drying 48 h of product, pulverize and sieve (100 order) obtain approximately 45 g of poly-2-acrylamide-2-methylpro panesulfonic acid powder.Get poly-2-acrylamide-2-methylpro panesulfonic acid powder 0.02 g, add 20 ml deionized waters, stir and pour in 9 cm culture dishes, standing 24 h obtain the high hydroscopic resin germinating bed that thickness is 0.3cm.
Embodiment 2Seed germination experiment
Get 100, the large gold dollar seed of safflower, 4 repetitions, evenly be placed in the 9 cm culture dishes that contain the rear high hydroscopic resin of suction of embodiment 1 preparation.Separately get 100 large gold dollar seeds of safflower, repeat for 4 times, evenly be placed in the 9 cm culture dishes (CK) in contrast that are lined with two-layer moistening filter paper.Then in illumination box 30 ℃ of (8 h) nights on daytime 20 ℃ of (16 h) alternating temperatures, 16 d that germinate.Add 1 ml deionized water to keep filter paper moistening every day in paper bed culture dish, the high hydroscopic resin germinating bed no longer keeps the skin wet.Record the chitting piece number every day, the 16th d calculates germination rate, germination index (GI) and vitality index (VI), computing formula: GI=∑ (Gt/Dt), VI=S * GI, in formula: Gt is the chitting piece number of t days, and Dt is the germination number of days, and S is full stand long (mm).
Embodiment 3Seed germination experiment
Repeat embodiment 2, following difference is arranged: change the large gold dollar seed of safflower into MS K326 seed.
Embodiment 4The growth of seedling test
Get 100, the large gold dollar seed of safflower, 4 repetitions, evenly be placed in the 9 cm culture dishes that contain the rear high hydroscopic resin of suction of embodiment 1 preparation.Separately get 100 large gold dollar seeds of safflower, repeat for 4 times, evenly be placed in the 9 cm culture dishes (CK) in contrast that are lined with two-layer moistening filter paper.Then in illumination box 30 ℃ of (8 h) nights of daytime 20 ℃ of (16 h) Fluctuation temperature culture 30 d.Add 1 ml deionized water to keep filter paper moistening every day in paper bed culture dish, the high hydroscopic resin germinating bed no longer keeps the skin wet.Get the seedling of growth 30 d, blot with filter paper after rinsing with running water.30 strains are chosen in each processing at random, measure the length of its root and overground part with ruler, are accurate to mm; With one thousandth sensibility reciprocal balance weighing full stand fresh weight, seedling is dried 24 h under 80 ℃, measure the full stand dry weight, be accurate to mg.
Embodiment 5The growth of seedling test
Repeat embodiment 4, following difference is arranged: change the large gold dollar seed of safflower into MS K326 seed.
The large gold dollar of safflower and MS K326 seed are placed on the prepared water-absorbing resin of embodiment 1 and germinate, and result of the test is carried out
tTest (α=0.05) and show, the germination rate of seed, germination index and vitality index are compared all without significant difference (table 1, table 2) with contrast (germinateing on paper).The large gold dollar seed of safflower is grown on the prepared water-absorbing resin of embodiment 1, the seedling root is long, full stand is long, the full stand fresh weight is compared (table 3) all without significant difference with the full stand dry weight with contrast (growing on paper); MS K326 seed is grown on the prepared water-absorbing resin of embodiment 1, the seedling root is long significantly lower than contrast, and the seedling overground part is high, full stand fresh weight and full stand dry weight be compared with the control all without significant difference (table 4).
The variation of the large gold dollar seed of table 1 tobacco bred safflower germination rate, germination index and vitality index on different germinating beds
| Germination condition | Germination rate (%) | Germination index (GI) | Vitality index (VI) |
| Germinate on paper (CK) | 96.7a * | 17.72a | 27.12a |
| Water-absorbing resin **Upper germination | 96.3a | 16.58a | 25.83a |
*Lowercase represent between different disposal the significance of difference relatively (α=0.05, LSD), lower with.
*Water-absorbing resin refers to the poly-2-acrylamide-2-methyl propane sulfonic (PAMPS) of 0.1% concentration, and is lower same.
The variation of table 2 tobacco bred MS K326 seed germination rate, germination index and vitality index on different germinating beds
| Germination condition | Germination rate (%) | Germination index (GI) | Vitality index (VI) |
| On paper (CK) | 92.7a * | 16.49a | 17.58a |
| Water-absorbing resin ** | 92.0a | 16.27a | 16.98a |
The large gold dollar of table 3 tobacco bred safflower grows on different germinating beds that root is long, overground part is high, full stand fresh weight and full stand dry weight change
Table 4 tobacco bred MS K326 grows on different germinating beds that root is long, overground part is high, full stand fresh weight and full stand dry weight change
The seed sprouting bed of the present invention's preparation, except the growth to the seedling root has remarkable inhibitory action, all do not make significant difference to the growth of seedling overground part, seedling fresh weight and dry weight and seed sprouting situation.Therefore, the prepared seed sprouting bed of the present invention can be used for the indexs such as germination rate, germination index and vitality index of seed are detected, and the use value of seed lot is assessed, and can meet the needs in production.The poly-2-acrylamide-2-methylpro panesulfonic acid of the core material that the present invention uses utilizes cheap raw material preparation, and the preparation method is simple and easy, and the poly-2-acrylamide-2-methylpro panesulfonic acid hydrogel after use can absorb water after drying again, Reusability.Poly-2-acrylamide-2-methylpro panesulfonic acid hydrogel is transparence, can clearly observe the metamorphosis of root in process of growth.In addition, poly-2-acrylamide-2-methylpro panesulfonic acid has good retaining water retaining function, and therefore, whole germination process does not need regular replenishment moisture, can save a large amount of manpowers.
Claims (3)
1. the preparation method of a novel tobacco seed germination bed comprises the following steps:
1): N
2Add successively 100 g deionized waters, 35 g 2-acrylamide-2-methylpro panesulfonic acids, 12 g sodium hydroxide, 0.014 g potassium peroxydisulfate and 0.105 g N at 500 mL round-bottomed flasks under protection, N '-60 ℃ of methylene-bisacrylamides heat temperature raising reaction, 12 h, 60 ℃ of drying 48 h of product, pulverized 100 mesh sieves and obtained approximately 45 g of poly-2-acrylamide-2-methylpro panesulfonic acid powder;
2): a certain amount of poly-2-acrylamide-2-methylpro panesulfonic acid powder is dissolved in deionized water, stirs, the solution concentration that obtains is 0.1%;
3): the solution of 10～100mL step 2 preparation is poured in the germination box or culture dish of different size, and standing 24 h obtain gelatinous high hydroscopic resin germinating bed.
2. preparation method as claimed in claim 1, is characterized in that described germination box comprises the plastics germination box of 12 * 12cm and 24 * 12cm, and culture dish comprises glass or the plastic culture dish of diameter 6cm～18cm.
3. preparation method as claimed in claim 1, is characterized in that, if give thousand kernel weight when the minimum grain Tobacco Seed Germination of 0.05 ~ 0.5g, the thickness of bud bed is 0.2 ~ 0.5cm.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104445161A CN102523786B (en) | 2011-12-27 | 2011-12-27 | Preparation method for novel tobacco seed germination bed |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104445161A CN102523786B (en) | 2011-12-27 | 2011-12-27 | Preparation method for novel tobacco seed germination bed |
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| CN102523786A CN102523786A (en) | 2012-07-04 |
| CN102523786B true CN102523786B (en) | 2013-11-20 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109593169A (en) * | 2018-11-28 | 2019-04-09 | 河南农业大学 | A method of Tobacco Seed Germination bed is prepared using offal |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102726147B (en) * | 2012-07-12 | 2013-12-11 | 贵州省烟草科学研究所 | Tobacco seed germinating bed and preparation method thereof |
| CN109503779A (en) * | 2018-11-28 | 2019-03-22 | 河南农业大学 | A method of Tobacco Seed Germination bed is prepared using tabacco straw |
| CN109575200B (en) * | 2018-11-28 | 2021-01-15 | 河南农业大学 | Method for preparing tobacco seed germinating bed by using corn straws |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| FR2671455B1 (en) * | 1991-01-10 | 1993-04-30 | Ind Tabacs Allume Exploit | IMPROVEMENT IN THE PRODUCTION OF TOBACCO PLANTS ACCORDING TO THE FLOATING SYSTEM. |
| CN101611663A (en) * | 2009-07-24 | 2009-12-30 | 中国烟草总公司黑龙江省公司牡丹江烟草科学研究所 | A kind of tobacco seed germination accelerating method |
| CN201766827U (en) * | 2010-08-13 | 2011-03-23 | 云南省烟草农业科学研究院 | Germination test bed for small plant seeds |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109593169A (en) * | 2018-11-28 | 2019-04-09 | 河南农业大学 | A method of Tobacco Seed Germination bed is prepared using offal |
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