CN106416507A - Method for improving cornus controversa seed germination rate - Google Patents
Method for improving cornus controversa seed germination rate Download PDFInfo
- Publication number
- CN106416507A CN106416507A CN201610864624.7A CN201610864624A CN106416507A CN 106416507 A CN106416507 A CN 106416507A CN 201610864624 A CN201610864624 A CN 201610864624A CN 106416507 A CN106416507 A CN 106416507A
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- China
- Prior art keywords
- seed
- germination
- cornus controversa
- clear water
- germination percentage
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
Abstract
The invention discloses a method for improving cornus controversa seed germination rate, and belongs to the field of planting. The method specifically includes the following steps of collection and preparation of seeds, seed disinfection and soaking treatment and composite germination accelerating treatment. According to the method, removal of base seed coat through mechanical disruption, sand storage treatment for a long time and other time-consuming and effort-consuming operating modes are not required, germination of the seeds can be accelerated, and the germination rate is increased to be 95% or above. Compared with an existing method, the germination rate is increased by 20% or above, and the germination time is shortened by about 7 days. In addition, under synthetic combination of all steps, the characteristics of the seeds are improved, the quality of the cultivated seedlings is also improved correspondingly, the finally cultivated cornus controversa is higher in scholaricine content and has better use value.
Description
Technical field
The invention belongs to field of planting is and in particular to a kind of method of raising lampstand tree seed germination percentage.
Background technology
Cornus controversa（Classification system：Bothrocaryum controversum）, call auspicious wood, Nv Ermu, hexagonal tree.It is
Cornaceae, lamp stand Pterostyrax deciduous tree, high 6-15 rice, dilute reach 20 meters；Bark is smooth, lead or band yellow-gray；Branch is carried out,
Cylinder, no hair or thin raw pubescence, the purplish red green of annual shoot, biennial branch light green, have semi-moon shaped leaf scar and circle
Hole skin.It is distributed in each province on the south Liaoning, Hebei, Shaanxi, Gansu, Shandong, Anhui, Taiwan, Henan, Guangdong, Guangxi and the Changjiang river
Area.
Cornus controversa has higher economic worth, early 1970s, the traditional ethnic minority medicine scientific research personnel in Yunnan Province
After medicine, pharmacological experiment study it was demonstrated that the root of Cornus controversa, bark, leaf and seed kind all containing can hyoscine lamp stand
Alkali, lamp stand determine alkali, and non-nitrogen material, can be used for treating cold, pneumonia, pertussis, chronic bronchitis etc., effect shows
Write.External application can treat wound, synthetism, detumescence etc..With the substantial amounts of exploitation of people, wild Cornus controversa resource is increasingly
Less it has to carry out artificial cultivation to meet growing demand.Existing multiplex seed carries out nursery plantation, but because its kind of skin
The impact of the composite factors such as fine and close, hard, ventilating permeable lid, the natural germination percentage of seed is relatively low, and normally no higher than 50%, because
And need to carry out corresponding Seed Treatment, vernalization operation although the low phenomenon of germination percentage can be improved now with a lot of methods, but still
Do not reach it is intended that level.
Content of the invention
The present invention is intended to provide a kind of method improving lampstand tree seed germination percentage.
The present invention is achieved through the following technical solutions：
A kind of method improving lampstand tree seed germination percentage, comprises the steps：
（1）Prepared by seed collection：
When Cornus controversa pericarp switchs to green and brown color by green, by Cornus controversa fruit harvesting, put in clear water and soak, macerate rotten rear stranding with the hands and go
Exocarp and pulp, then the salt solution for 0.2 ~ 0.3% dries after cleaning up by drupe mass fraction, prepared seed is standby
With；
（2）Sterilization seed soaking：
By step（1）Prepared seed puts into immersion treatment 1 ~ 1.2h in the liquor potassic permanganate that volumetric concentration is 0.3 ~ 0.4%
Afterwards, taking-up clear water is rinsed well, then puts it into immersion 5 ~ 7h in the Gibberellins solution that concentration is 250 ~ 350mg/L, finally
It is drawn off being rinsed well with clear water standby；
（3）Compound vernalization is processed：
A. by step（2）Seed after process is put in insulating box, keeps the temperature in case to be 35 ~ 40 DEG C, relative air humidity
For 65 ~ 75%；
B. apply blue light illumination to seed in insulating box to process simultaneously；Illumination is the key factor that impact seed is sprouted,
People typically seek optimal light quality and are irradiated processing, but find although ruddiness and gold-tinted are for lamp stand in actual applications
The sprouting facilitation effect of seeds is higher compared with blue light, but cannot be further continued for after reaching certain bottleneck being lifted, and selects blue light to process,
Cooperation has the ultrasonication of stronger seed modifying function, has good synergy, and ruddiness or gold-tinted do not reach
This effect, may is that ruddiness, gold-tinted are different from the mechanism of action of blue light, blue light improves the moieties that the above two cannot be lifted
Activity, and the inhibitory action for most materials has been carried out making up, has been improved by ultrasonic wave, and then overcomes the bottleneck of lifting
Problem；
C. also carry out ultrasonication in insulating box simultaneously；Ultrasonic wave can improve the activity of the materials such as seed majority enzyme, promotees
Its sprouting, the use of cooperation blue light are entered, effect is more excellent；
D. after being jointly processed by 8 ~ 10h, seed is taken out and can sow.
Further, step（3）The wavelength of the blue light described in operation b is 420 ~ 460nm.
Further, step（3）The frequency of the ultrasonic wave described in operation c is 26 ~ 28kHz.
The present invention has the advantages that：
The immersion treatment by Gibberellins solution for the present invention, and special compound vernalization process, improve Cornus controversa well
The characteristic of seed, has broken its dormancy well, promotes its sprouting, do not need to carry out mechanical damage remove base portion kind skin,
The mode of operation that Sand culture etc. takes time and effort for a long time, just can accelerate the sprouting of seed, and germination percentage is promoted to 95%
More than, the germination percentage comparing existing method improves more than 20%, and germinating time correspondence shortens 7 days about.Additionally, in each step
Under rapid synthesis cooperation, the characteristic of seed is improved, and raised an infant seedling body matter also obtains corresponding change, the final Cornus controversa being bred as
In lamp stand alkali content higher, have more preferable use value.
Specific embodiment
Embodiment 1
A kind of method improving lampstand tree seed germination percentage, comprises the steps：
（1）Prepared by seed collection：
When Cornus controversa pericarp switchs to green and brown color by green, by Cornus controversa fruit harvesting, put in clear water and soak, macerate rotten rear stranding with the hands and go
Exocarp and pulp, then dry after the salt solution that drupe mass fraction is 0.2% is cleaned up, prepared seed is standby；
（2）Sterilization seed soaking：
By step（1）After prepared seed puts into immersion treatment 1h in the liquor potassic permanganate that volumetric concentration is 0.3%, take out and use
Clear water is rinsed well, then puts it into immersion 5h in the Gibberellins solution that concentration is 280mg/L, is finally drawn off being rushed with clear water
Wash clean is standby；
（3）Compound vernalization is processed：
A. by step（2）Seed after process is put in insulating box, keeps the temperature in case to be 37 DEG C, relative air humidity is 65
~70%；
B. apply blue light illumination to seed in insulating box to process simultaneously；
C. also carry out ultrasonication in insulating box simultaneously；
D. after being jointly processed by 8.5h, seed is taken out and can sow.
Further, step（3）The wavelength of the blue light described in operation b is 420 ~ 450nm.
Further, step（3）The frequency of the ultrasonic wave described in operation c is 26kHz.
Embodiment 2
A kind of method improving lampstand tree seed germination percentage, comprises the steps：
（1）Prepared by seed collection：
When Cornus controversa pericarp switchs to green and brown color by green, by Cornus controversa fruit harvesting, put in clear water and soak, macerate rotten rear stranding with the hands and go
Exocarp and pulp, then dry after the salt solution that drupe mass fraction is 0.3% is cleaned up, prepared seed is standby；
（2）Sterilization seed soaking：
By step（1）After prepared seed puts into immersion treatment 1.2h in the liquor potassic permanganate that volumetric concentration is 0.4%, take out
Rinsed well with clear water, then put it into immersion 7h in the Gibberellins solution that concentration is 350mg/L, be finally drawn off using clear water
Rinse well standby；
（3）Compound vernalization is processed：
A. by step（2）Seed after process is put in insulating box, keeps the temperature in case to be 39 DEG C, relative air humidity is 70
~75%；
B. apply blue light illumination to seed in insulating box to process simultaneously；
C. also carry out ultrasonication in insulating box simultaneously；
D. after being jointly processed by 10h, seed is taken out and can sow.
Further, step（3）The wavelength of the blue light described in operation b is 430 ~ 460nm.
Further, step（3）The frequency of the ultrasonic wave described in operation c is 28kHz.
Comparative example 1
This comparative example 1 compared with Example 1, blue light is substituted for ruddiness, method and step all same in addition.
Comparative example 2
This comparative example 2 compared with Example 2, in step（3）Compound vernalization does not carry out ultrasonication operation when processing.
Control group
The husky Tibetan of existing lampstand tree seed, HORMONE TREATMENT germination method.
In order to contrast effect of the present invention, from a collection of lampstand tree seed, carry out seed with above-mentioned five kinds of methods respectively and sprout
Send out and process, and record corresponding data, specifically see table：
| Every gram of Conductivity（μS/（cm·g）） | Germination percentage（%） | Germinate average duration（My god） | |
| Embodiment 1 | 1.9 | 95.7 | 2.6 |
| Embodiment 2 | 1.8 | 96.1 | 2.5 |
| Comparative example 1 | 2.2 | 90.8 | 3.4 |
| Comparative example 2 | 2.5 | 83.3 | 7.7 |
| Control group | 2.7 | 78.5 | 10.4 |
Note：Measurement Conductivity in upper table is to carry out before germination, and it has reacted the activity of seed, and activity is more high right
The conductivity values answered are lower；The average duration that germinates refers to complete, with a collection of Tests Seeds, the required average duration that germinates.
As can be seen from the above table, the inventive method can strengthen the activity of seed, the germination percentage of lifting seed well, and contracts
The short duration germinateing.
In order to contrast effect of the present invention further, the chitting piece after said method alignment processing is carried out sowing, cultivates,
Management method etc. is identical and suitable, until being bred as the seedling of a height of 50 ~ 55cm, and carries out alkaloid to the blade of seedling and contains
Measure examination, following table is correction data：
| Leaf total alkali degree（%） | |
| Embodiment 1 | 0.215 |
| Embodiment 2 | 0.223 |
| Comparative example 1 | 0.193 |
| Comparative example 2 | 0.162 |
| Control group | 0.138 |
By upper table it is found that the Cornus controversa Plant Leaf total alkali of the inventive method incubation is higher, there is more preferable use value.
Claims (3)
1. a kind of method improving lampstand tree seed germination percentage is it is characterised in that comprise the steps：
（1）Prepared by seed collection：
When Cornus controversa pericarp switchs to green and brown color by green, by Cornus controversa fruit harvesting, put in clear water and soak, macerate rotten rear stranding with the hands and go
Exocarp and pulp, then the salt solution for 0.2 ~ 0.3% dries after cleaning up by drupe mass fraction, prepared seed is standby
With；
（2）Sterilization seed soaking：
By step（1）Prepared seed puts into immersion treatment 1 ~ 1.2h in the liquor potassic permanganate that volumetric concentration is 0.3 ~ 0.4%
Afterwards, taking-up clear water is rinsed well, then puts it into immersion 5 ~ 7h in the Gibberellins solution that concentration is 250 ~ 350mg/L, finally
It is drawn off being rinsed well with clear water standby；
（3）Compound vernalization is processed：
A. by step（2）Seed after process is put in insulating box, keeps the temperature in case to be 35 ~ 40 DEG C, relative air humidity
For 65 ~ 75%；
B. apply blue light illumination to seed in insulating box to process simultaneously；
C. also carry out ultrasonication in insulating box simultaneously；
D. after being jointly processed by 8 ~ 10h, seed is taken out and can sow.
2. a kind of method improving lampstand tree seed germination percentage according to claim 1 is it is characterised in that step（3）Behaviour
The wavelength making the blue light described in b is 420 ~ 460nm.
3. a kind of method improving lampstand tree seed germination percentage according to claim 1 is it is characterised in that step（3）Behaviour
The frequency making the ultrasonic wave described in c is 26 ~ 28kHz.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610864624.7A CN106416507A (en) | 2016-09-29 | 2016-09-29 | Method for improving cornus controversa seed germination rate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610864624.7A CN106416507A (en) | 2016-09-29 | 2016-09-29 | Method for improving cornus controversa seed germination rate |
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| CN106416507A true CN106416507A (en) | 2017-02-22 |
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| CN201610864624.7A Pending CN106416507A (en) | 2016-09-29 | 2016-09-29 | Method for improving cornus controversa seed germination rate |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107484464A (en) * | 2017-09-27 | 2017-12-19 | 无为县雲桃家庭农场 | A kind of method for promoting Fruit Tree Seeds fast germination |
| CN111631068A (en) * | 2020-06-11 | 2020-09-08 | 淮南师范学院 | Cultivation method of lampstand tree |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1409951A (en) * | 2002-10-18 | 2003-04-16 | 中国科学院西双版纳热带植物园 | Collecting and storing method of lampstand tree seed |
-
2016
- 2016-09-29 CN CN201610864624.7A patent/CN106416507A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1409951A (en) * | 2002-10-18 | 2003-04-16 | 中国科学院西双版纳热带植物园 | Collecting and storing method of lampstand tree seed |
Non-Patent Citations (2)
| Title |
|---|
| 蔡传涛 等: "灯台树种子萌发特性的研究", 《中草药》 * |
| 陈军 等: "灯台树的生物学特性与繁殖方法", 《特种经济动植物》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107484464A (en) * | 2017-09-27 | 2017-12-19 | 无为县雲桃家庭农场 | A kind of method for promoting Fruit Tree Seeds fast germination |
| CN111631068A (en) * | 2020-06-11 | 2020-09-08 | 淮南师范学院 | Cultivation method of lampstand tree |
| CN111631068B (en) * | 2020-06-11 | 2022-05-17 | 淮南师范学院 | Cultivation method of lampstand tree |
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Application publication date: 20170222 |