CN102726147A - Tobacco seed germinating bed and preparation method thereof - Google Patents
Tobacco seed germinating bed and preparation method thereof Download PDFInfo
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- CN102726147A CN102726147A CN201210241149XA CN201210241149A CN102726147A CN 102726147 A CN102726147 A CN 102726147A CN 201210241149X A CN201210241149X A CN 201210241149XA CN 201210241149 A CN201210241149 A CN 201210241149A CN 102726147 A CN102726147 A CN 102726147A
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Abstract
The invention discloses a tobacco seed germinating bed and a preparation method thereof. The tobacco seed germinating bed provided by the invention is an agar bed with the concentration of 5-11%. The preparation method of the germinating bed comprises the following steps of: vertically fixing at least one stick-like object to a plate-like object, thereby obtaining an agar puncher; inserting the stick-like object of the agar puncher into a container for holding agar downwards, and remaining a gap between the plate-like object of the puncher and the wall of the container; and then injecting liquid agar into the container, cooling and pulling out the puncher. The agar bed is used as the tobacco seed germinating bed in stead of the traditional paper bed, so that the defect that the paper bed needs to replenish water frequently is overcome and the seeding and counting are easy to realize in comparison with the paper bed; the preparation method of the agar bed is simple and easy to operate; and the working efficiency is greatly improved.
Description
Technical field
The present invention relates to a kind of tobacco seed germinating bed and preparation method thereof.
Background technology
Traditional germinating bed mainly comprises the paper bed, and husky bed and soil bed are mainly selected the paper bed for use on tobacco seed.Yet the paper bed has a lot of shortcomings.At first, paper bed poor water retention property is normally germinateed in order to guarantee seed, and we often need artificial recharge in 1 round of visits; If disposable to add water too many, pelleted seed can change into one again, is unfavorable for counting.Secondly, the paper bed does not possess adsorptivity, bring many difficulties for the seed program request, and pelleted seed rolls easily on the paper bed, and prepositioned kind bed destroys easily, causes the counting difficulty.
Summary of the invention
The purpose of this invention is to provide a kind of tobacco seed germinating bed and preparation method thereof.
For solving the problems of the technologies described above, technical scheme of the present invention is:
A kind of tobacco seed germinating bed is the agar bed.
In order to help the germination of seed, preferred, the concentration of agar bed is 5-11%.
In order to guarantee dissimilar seed germination rate, preferred, when seed is naked kind or causes kind of a period of the day from 11 p.m. to 1 a.m, the concentration of agar bed is 5-8%, and when seed was pelleted seed, the concentration of agar bed was 8-11%.
More preferably, when seed is naked kind or causes kind of a period of the day from 11 p.m. to 1 a.m, the concentration of agar bed is 8%, and when seed was pelleted seed, the concentration of agar bed was 11%.
The percentage sign of the concentration of above-mentioned agar is represented in every 100ml buffer solution, and the gram number of agar powder contains the agar powder of 1g in the buffer solution like 1% expression 100ml.
The preparation method of above-mentioned germinating bed comprises the steps:
A, at least one rod shape thing is vertically fixed on the plate object, Gelose punch;
B, with above-mentioned Gelose punch, rod shape thing inserts downwards and holds in the container of agar, and leaves the space between the plate object of card punch and the chamber wall;
C, be the liquid agar of 5-11% with implantation concentration in the said vesse, card punch is extracted in cooling, germinating bed.
The above-mentioned container that holds agar is generally culture dish.
For the ease of sowing, preferred, rod shape thing is cylindrical in the steps A.
In order to save the space, improve germination rate simultaneously, preferred, the diameter of the cross section of rod shape thing is 0.5-1.5mm in the steps A.
In order to save the space, guarantee germination rate simultaneously, rod shape thing is two when above in the steps A, the distance between the adjacent two rod shape things is preferably 3-8mm.
In order to improve statistical accuracy, preferred, the rod shape thing in the steps A on the plate object is linearity, rectangle, rhombus or circular arrangement.
In order to improve statistical accuracy, preferred, rod shape thing is two when above in the steps A, and each rod shape thing geomery all is equal to.
More preferably, be evenly distributed with the 100 rod shape things with, rectangular arrangement in the steps A on the card punch, the beeline between the adjacent two rod shape things is 5mm.
Above-mentioned, the thick 5-7mm that is preferably of the liquid agar of injection among the step C.
In order to help the germination of seed, preferred, extract card punch among the step C after, the hole depth of staying on the agar bed is 0.5-1.5mm.
The present invention uses the agar bed to replace traditional paper bed, has not only overcome the defective of the diligent moisturizings of paper bed needs, and sows more easily, adds up with respect to the paper bed; The preparation method of agar bed is simple to operation, has improved operating efficiency significantly, and with low cost, and is suitable equally for the tobacco grower of remote mountain areas.
Description of drawings
Fig. 1 is a structural representation in the embodiment of the invention.
Above-mentioned, among Fig. 1,1 is rod shape thing, and 2 is plate object.
Embodiment
In order to understand the present invention better, further illustrate content of the present invention below in conjunction with embodiment, but content of the present invention not only is confined to following embodiment.
The seed that No. 3, K326 among the embodiment, cloud and mist 87 and Nan Jiang is preserved by Guizhou Province Tabacco Scientific Research Institute stock breeding popularization center and is provided.
Agar powder is sowed rich company by Shanghai and is produced, and gel strength is 900g/cm
2, total ash≤6.5%, heavy metal<40ppm, lead<10ppm.
The cultivating seeds temperature is 26 ℃, and relative moisture is greater than 90%, and cultivation in looking after the artificial climate incubator (RZH-850A) that replaces with dark 12h, and isometric with radicle and seed is the germination standard.
Naked kind germination is strict to be carried out according to industry standard YC/T20-1994, and capsuled seed, initiation seed are with reference to the provincial standard tobacco seed propagation production technical regulation DB53/T353-2011 of Yunnan Province.
Germination vigor is meant that when the Sino-Japan chitting piece number of germination process reaches the top seeds germinated number accounts for the percentage that supplies test sample article seed number.
Germination rate is meant seed in the fate of germination test initial stage regulation (when counting for the first time), and normal chitting piece number accounts for the percentage that supplies to plant experimentally subnumber.
Adopt SPSS16.0 to carry out variance analysis, the result is four mean values that repeat.
The specification of culture dish is diameter 90mm at the bottom of the ware, and the ware height is 15mm, and the ware base thickness is 2mm.
1, the preparation of card punch: in the length of side is on the square plank of 70mm, and fixing 10 row diameters are 1mm, and length is the rod shape thing of 8mm, fixing 10 of every row, and adjacent two every trade distances and adjacent two row row are apart from being 5mm.
2, above-mentioned card punch rod shape thing is inserted in the culture dish downwards.
3, adopt the SX-500 high-pressure sterilizing pot that agar powder is dissolved, get the agar solution of desired concn, in the culture dish in the gained agar solution implantation step 2; Make the thick 6mm of being of liquid agar; After the cooling, vertically extract card punch gently, on the agar bed, stayed 100 of planting holes.
Preparing concentration respectively according to said method is each two parts of 5.5%, 11%, 16.5%, 22%, 27.5% agar beds, naked kind and the pelleted seed of dibbling K326 in the hole on above-mentioned agar bed then.
2 filter paper of tiling drench filter paper with distilled water in culture dish, get the paper bed as contrast, and the seed amount of its dibbling and spacing are with the agar bed.
Add up seed germination rate in the above-mentioned germinating bed, specifically as shown in table 1.
Naked kind of table 1 and the pelleted seed germination on agar bed and paper bed
Table is annotated: in the same type seed of same kind, same columns value indicates different alphabetical a, b, c and representes to reach 0.5% significant difference, does not have alphabetical distance pole and representes that difference is not remarkable.
Further carry out the demonstration test of the dissimilar seeds of different cultivars with the seed of 3 types of 3 kinds; With reference to the method among the embodiment 1; Prepare concentration respectively and be each 9 parts of 8%, 11%, 14% agar beds, respectively the naked kind of No. 3, dibbling K326 in the hole on the agar bed, cloud and mist 87 and Nan Jiang, cause seed and capsuled seed.
2 filter paper of tiling drench filter paper with distilled water at the bottom of culture dish, get the paper bed as contrast, and the seed amount of its dibbling and spacing are with the agar bed.The result is as shown in table 2.Germination vigor was the 7th day statistics, and germination rate was the 14th day statistics.
3 types of seeds of 3 kinds of table 2 are in the germination of agar bed
Table is annotated: in the same type seed of same kind, same columns value indicates different alphabetical a, b, c and representes to reach 0.5% significant difference, does not have alphabetical distance pole and representes that difference is not remarkable.As: the germination vigor with the K326 capsuled seed is an example, and the germination vigor that is illustrated in seed on 11% the agar bed is significantly higher than 14%; But the paper bed, 8% agar bed, 11% agar bed does not have significant difference, and paper bed, 8% agar bed and 14% agar bed do not have significant difference yet.
Claims (11)
1. a tobacco seed germinating bed is characterized in that: be the agar bed of 5-11% for concentration.
2. germinating bed as claimed in claim 1 is characterized in that: when seed is naked kind and causes kind of a period of the day from 11 p.m. to 1 a.m, the concentration of agar bed is 5-8%, and when seed was pelleted seed, the concentration of agar bed was 8-11%.
3. the preparation method of claim 1 or 2 described germinating beds is characterized in that: comprise the steps:
A, at least one rod shape thing is vertically fixed on the plate object, Gelose punch;
B, with above-mentioned Gelose punch, rod shape thing inserts downwards and holds in the container of agar, and leaves the space between the plate object of card punch and the chamber wall;
C, be the liquid agar of 5-11% with implantation concentration in the said vesse, card punch is extracted in cooling, germinating bed.
4. method as claimed in claim 3 is characterized in that: rod shape thing is cylindrical in the steps A.
5. method as claimed in claim 4 is characterized in that: the diameter of the cross section of rod shape thing is 0.5-1.5mm in the steps A.
6. like any described method of claim 3 to 5, it is characterized in that: rod shape thing is two when above in the steps A, and the distance between the adjacent two rod shape things is 3-8mm.
7. like any described method of claim 3 to 5, it is characterized in that: rod shape thing is two when above in the steps A, and the rod shape thing on the plate object is linearity, rectangle, rhombus or circular arrangement.
8. like any described method of claim 3 to 5, it is characterized in that: rod shape thing is two when above in the steps A, and each rod shape thing geomery all is equal to.
9. method as claimed in claim 8 is characterized in that: be evenly distributed with the 100 rod shape things with, rectangular arrangement in the steps A on the card punch, the beeline between the adjacent two rod shape things is 5mm.
10. like any described method of claim 3 to 5, it is characterized in that: the thick 5-7mm of being that injects liquid agar among the step C.
11. like any described method of claim 3 to 5, it is characterized in that: after extracting card punch among the step C, the hole depth of staying on the agar bed is 0.5-1.5mm.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210241149XA CN102726147B (en) | 2012-07-12 | 2012-07-12 | Tobacco seed germinating bed and preparation method thereof |
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| CN201210241149XA CN102726147B (en) | 2012-07-12 | 2012-07-12 | Tobacco seed germinating bed and preparation method thereof |
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| CN102726147A true CN102726147A (en) | 2012-10-17 |
| CN102726147B CN102726147B (en) | 2013-12-11 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102939894A (en) * | 2012-11-15 | 2013-02-27 | 中国农业大学 | Seed germination device and method for detecting bean germination characters by same |
| CN107396823A (en) * | 2017-08-28 | 2017-11-28 | 上海大学 | The method that quickly breeding transplants submerged plant |
| CN111466177A (en) * | 2020-04-22 | 2020-07-31 | 内蒙古自治区农牧业科学院 | Agar gel germination bed, hole maker and seed germination method |
| CN111466176A (en) * | 2020-04-22 | 2020-07-31 | 内蒙古自治区农牧业科学院 | Alfalfa agar gel germination bed and alfalfa seed germination method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100984129B1 (en) * | 2010-07-08 | 2010-09-28 | 코스팜 주식회사 | Compressed sheet-type bed soil for nursing plant seedlings with good root growth |
| CN201766827U (en) * | 2010-08-13 | 2011-03-23 | 云南省烟草农业科学研究院 | Germination test bed for small plant seeds |
| CN102523786A (en) * | 2011-12-27 | 2012-07-04 | 云南省烟草农业科学研究院 | Preparation method for novel tobacco seed germination bed |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100984129B1 (en) * | 2010-07-08 | 2010-09-28 | 코스팜 주식회사 | Compressed sheet-type bed soil for nursing plant seedlings with good root growth |
| CN201766827U (en) * | 2010-08-13 | 2011-03-23 | 云南省烟草农业科学研究院 | Germination test bed for small plant seeds |
| CN102523786A (en) * | 2011-12-27 | 2012-07-04 | 云南省烟草农业科学研究院 | Preparation method for novel tobacco seed germination bed |
Non-Patent Citations (1)
| Title |
|---|
| 姚文彪: "用琼脂做烟草种子发芽床的试验", 《中国烟草科学》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102939894A (en) * | 2012-11-15 | 2013-02-27 | 中国农业大学 | Seed germination device and method for detecting bean germination characters by same |
| CN107396823A (en) * | 2017-08-28 | 2017-11-28 | 上海大学 | The method that quickly breeding transplants submerged plant |
| CN111466177A (en) * | 2020-04-22 | 2020-07-31 | 内蒙古自治区农牧业科学院 | Agar gel germination bed, hole maker and seed germination method |
| CN111466176A (en) * | 2020-04-22 | 2020-07-31 | 内蒙古自治区农牧业科学院 | Alfalfa agar gel germination bed and alfalfa seed germination method |
| CN111466177B (en) * | 2020-04-22 | 2023-08-04 | 内蒙古自治区农牧业科学院 | Agar gel germination bed, pore-forming device and seed germination method |
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| CN102726147B (en) | 2013-12-11 |
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