CN102939894A - Seed germination device and method for detecting bean germination characters by same - Google Patents
Seed germination device and method for detecting bean germination characters by same Download PDFInfo
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- CN102939894A CN102939894A CN201210459369XA CN201210459369A CN102939894A CN 102939894 A CN102939894 A CN 102939894A CN 201210459369X A CN201210459369X A CN 201210459369XA CN 201210459369 A CN201210459369 A CN 201210459369A CN 102939894 A CN102939894 A CN 102939894A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
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Abstract
The invention discloses a seed germination device and a method for detecting bean germination characters by applying the seed germination device. The seed germination device provided by the invention is obtained by solidifying an agar water solution and consists of a base and a plurality of side walls perpendicular to the base, wherein the base and every two adjacent side walls define a groove. The invention protects a method for cultivating seed germination, and seeds are arranged and cultivated in the grooves of the seed germination device. The invention also protects the seed germination device or the application of the method in detecting the bean germination characters. The method provided by the invention has the following advantages: (1) the operation is simple, and water does not need to be added in the germination process; (2) bean sprouts are not rotted or mildewed easily in the cultivation process; (3) the cultivated bean sprouts do not have lateral roots; (4) bean resources do not need to be pre-soaked, and dry beans are directly cultivated; and (5) the method is rapid and efficient, and the needed amount of bean seeds is small.
Description
Technical field
The invention belongs to seed quality and detect and the screening field, be specifically related to a kind of seed sprouting device and use the method that it detects the bean germination proterties.
Background technology
The bean sprouts has another name called bud seedling dish, is edible " bud dish " that the cultivating seeds of various beans goes out, also claims " live body vegetables ".There are moyashi, mung bean sprouts, black bean sprout etc. in more common bean sprouts.
Soybean claims soya bean usually, though its protein content is high, because it exists trypsin inhibitor, its nutritive value is restricted, so people advocate edible bean product.Soya bean in germination process, the trypsin inhibitor major part destruction that is degraded.The protein utilization of moyashi will improve about 10% than soya bean.In addition, what contain in the soya bean can not be absorbed by the body, and easily causes again the materials such as raffinose of abdominal distension, sharply descends in germination process and even all disappears, and this has just been avoided eating the generation of physogastry behind the soya bean.Soya bean is in germination process, because the effect of enzyme, the mineral matter elements such as more calcium, phosphorus, iron, zinc are released, and this has increased again the human body availability of soya bean mineral.The soya bean rear asparatate of sprouting sharply increases, and can reduce in the body lactic acid and piles up so the people eats the bean sprouts, eliminates tired.In recent years find to contain in the bean sprouts a kind of interferon and give birth to agent, the energy inducement interferon increases antibiotic in the body, increases interior resisting virus, anticancer swollen ability.
The traditional Chinese medical science thinks, it is cool in nature for mung bean sprouts, flavor is sweet nontoxic, can affix one's name to clearly heat, transfers the five internal organs, separate all poison, promoting urination and removing dampness, and it is tired to can be used for insobriety, damp and hot stasis, the few body of food.Hypertension and patients with coronary heart disease can often be eaten element summer and fry mung bean sprouts.Among the people stewing with crucian with mung bean sprouts takes, and controls agalactia.Mung bean sprouts is squeezed the juice, and adds white sugar for tea-drinking, controls the diseases such as urinary tract infections, urine red heat, frequent micturition.The Japan scientist finds that in the mung bean germination process, partially protein can be decomposed into amino acid, thereby increases original amino acid content.Wherein also contain cellulose, if with leek with frying or cold and dressed with sauce, be used for old age and child's constipation, not only safety but also good effect is arranged.Take food mung bean sprouts, can treat vitamin A for want of and the nyctotyphlosis that causes, be deficient in vitamin B2 and scurvy that the tongue boil stomatitis that causes and scrotitis, the C that is deficient in vitamin cause etc.
Because the edibility of bean sprouts is huge, it is especially important that screening has the kind of merit.The method of existing detection bean germination proterties mainly is that germinating bed is positioned in the germination box, beans material to be measured is placed at the interval on germinating bed, then the germination box is placed culturing room to cultivate (incubation is observed the wettability of germinating bed and in time added water to keep germinating bed moistening toward the germination box), the bean sprouts is grown and is to a certain degree measured afterwards each characteristic index.The germination proterties of using said method detection beans has following limitation: need repeatedly to add water in the germination box in (1) experimentation, more loaded down with trivial details, and amount of water is difficult to mate fully with the demand of beans to be measured, as add the water deficiency and will cause the characteristic index that finally obtains to react the true quality of beans to be measured, as add dilutional hyponatremia and can cause in the bean germination process to be measured and corruption occurs; (2) in the experimentation, because excess moisture in the germination box, beans to be measured grows lateral root (common germination is anhydrous, and root is grown) usually downwards; (3) need in the experimentation to soak seed first, then trickle is cultivated the bean sprouts.
Summary of the invention
The purpose of this invention is to provide a kind of seed sprouting device and use the method that it detects the bean germination proterties.
Seed sprouting device provided by the invention is solidified by aqueous agar solution and to obtain, and forms by base with perpendicular to some sidewalls of base, forms 1 groove between base and per two adjacent walls.Because the seed sprouting device solidifies aqueous agar solution and obtains, contain sufficient moisture, in the process of cultivating seed sprouting, need not to water.
The preparation method of described aqueous agar solution is as follows: with 1.0-1.4g(such as 1.0g-1.2g, 1.2-1.3g or 1.3-1.4g) agar and 100mL water mixes, and obtains aqueous agar solution.
The degree of depth of described groove can be 0.3-1.0cm(such as 0.3-0.6cm or 0.6-1.0cm), width is 0.3-0.5cm(such as 0.3-0.4cm or 0.4-0.5cm).
Described base can be rectangle.
The length of each groove of described seed sprouting device is all consistent with the width of described base.
The degree of depth of each groove of described seed sprouting device and width equate.
The present invention also protects a kind of method of cultivating seed sprouting, is seed is placed the groove of above arbitrary described seed sprouting device and to cultivate.
Described seed is legume-seeds, such as soybean or mung bean.
The condition of described cultivation is: 20-25 ℃, 60%-95% humidity lucifuge were cultivated 4-6 days.The interval of adjacent seed specifically can be 0.2-0.5cm.
The condition of described cultivation specifically can be: 23+2 ℃, 85+2% humidity lucifuge were cultivated 6 days.
The present invention also protects above arbitrary described seed sprouting device or the application of above arbitrary described method in detecting the bean germination proterties.
Method provided by the invention is legume-seeds to be measured to be placed the sowing groove of agar medium and cultivate its bud proterties of mensuration after it germinates.Method provided by the invention has following advantage: (1) easy and simple to handle, need not to add water in germination process; (2) bean sprouts is difficult for corrupt, mildew and rot in the incubation; (3) the not long lateral root in bean sprouts of cultivating; (4) need not the beans resource is carried out in advance seed soaking, directly dried bean or pea are cultivated; (5) quick, efficient, required legume-seeds amount is few.
Description of drawings
Fig. 1 is the structural representation of seed sprouting device provided by the invention.
Embodiment
Following embodiment is convenient to understand better the present invention, but does not limit the present invention.Experimental technique among the following embodiment if no special instructions, is conventional method.Used test material among the following embodiment if no special instructions, is and purchases available from routine biochemistry reagent shop.Quantitative test in following examples all arranges repeated experiments three times, results averaged.Germination appliance used among the embodiment is all sterilized with 84 thimerosals.Beans seeds among the embodiment is full seed.Humidity in this patent, implication is relative moisture, i.e. the percentage of water in air vapour pressure and saturation vapour pressure.
Soya bean kind " 9395 ": the prosperous agricultural product of Heilungkiang Hailun City Co., Ltd.
Mung bean variety " No. 1, medium green " (seed properties and bud with characteristic preferably traditional bud mung bean variety): Institute of Crop Science, Chinese Academy of Agricultural Science.
Bean sprouts output ratio: the ratio of bean sprouts and material bean quality.
Root long (from the tip of a root to the root with the distance between the hypocotylar joint portion): it is long to adopt ruler to measure root, and unit is cm, the reservation two-decimal.
Hypocotyl length (distance from root and hypocotylar binding site to the sprout shoulder): adopt ruler to measure hypocotyl length, unit is cm, keeps two-decimal.
Hypocotyl thick (the hypocotyl rugosity at the upper 1cm place of root and hypocotyl binding site): adopt from aobvious slide measure, it is thick to measure each sprout, and unit is mm, keeps two-decimal.
Sprout fresh weight (mean fresh of single bean sprouts): with normal sprout counting, adopt analytical balance to weigh, unit is g, keeps three decimals, calculates the weight (g/) of single body.
Cotyledon is long: it is long to adopt ruler to measure cotyledon, and unit is cm, keeps two-decimal.
The preparation of embodiment 1, seed sprouting device
1,1.0g, 1.2g, 1.3g or 1.4g agar are mixed with 100mL water respectively, heating makes the agar dissolving, obtains agar solution.
2, respectively the agar solution of each concentration of step 1 preparation is poured in the different germination boxes (rectangular-shaped plastic casing), inserted space bar, room temperature leaves standstill until agar solution solidifies fully, extracts space bar, takes out the agar that solidifies, and is the seed sprouting device.
The seed sprouting device forms by the rectangle base with perpendicular to some sidewalls of base, form 1 groove between base and per two adjacent walls, the length of each groove is all consistent with the width of described base, the degree of depth of each groove is consistent, be 0.3-1.0cm, the width of each groove unanimously, be 0.3-0.5cm.
Embodiment 2, application seed sprouting device carry out germination test
1, the preparation of seed sprouting device
1.4g agar and 100mL water are mixed, and heating makes the agar dissolving, obtains agar solution.Method by embodiment 1 prepares the seed sprouting device and carries out germination test.The gash depth of seed sprouting device is that 1.0cm, width are 0.5cm.
2, use the seed sprouting device and carry out germination test
Be placed on the sowing groove interior (on the degree of depth of groove, seed is positioned at the centre position) of seed sprouting device after the seed disinfection with soya bean kind " 9395 " or mung bean variety " No. 1, medium green ".Cultivated 6 days under 23+2 ℃, 85+2% humidity, lucifuge condition, need not to add water in the incubation.If 3 groups of repeated experiments, every group of reconstruction experiment comprises two row grooves, places 10 seeds in every row groove, adjacent seed be spaced apart 0.5cm.
After soya bean was cultivated and finishes: ratio corrupt, that go rotten occurs in the bean sprouts was 0%, and the bean sprouts is not long lateral root all, and the ratio of hardseed is 15%, and ratio short and small, lopsided bud is 2%; Root is long to be that 4.68cm, hypocotyl length are that 13.89cm, hypocotyl slightly are 2.02cm; Output ratio is 5.03.
After mung bean was cultivated and finished: ratio corrupt, that go rotten occurs in the bean sprouts was 0%; Root is long to be that 5.87cm, hypocotyl length are that 10.39cm, hypocotyl slightly are 1.83cm; Output ratio is 5.38.
Embodiment 3, application seed sprouting device carry out germination test
1, the preparation of seed sprouting device
1.3g agar and 100mL water are mixed, and heating makes the agar dissolving, obtains agar solution.The step 1 of the other the same as in Example 2.The gash depth of seed sprouting device is that 0.3cm, width are 0.3cm.
2, use the seed sprouting device and carry out germination test
Step 2 with embodiment 2.
After mung bean was cultivated and finished: the root of bean sprouts was long for 5.99cm, hypocotyl length are that 11.36cm, hypocotyl slightly are 1.74cm, and output ratio is 5.58.
After soya bean was cultivated and finishes: the root of bean sprouts was long for 4.72cm, hypocotyl length are that 13.89cm, hypocotyl slightly are 2.12cm, and output ratio is 5.10.
Embodiment 4, application seed sprouting device carry out germination test
1, the preparation of seed sprouting device
1.2g agar and 100mL water are mixed, and heating makes the agar dissolving, obtains agar solution.The step 1 of the other the same as in Example 2.The gash depth of seed sprouting device is that 0.6cm, width are 0.4cm.
2, use the seed sprouting device and carry out germination test
Step 2 with embodiment 2.
After mung bean was cultivated and finished: the root of bean sprouts was long for 6.75cm, hypocotyl length are that 11.94cm, hypocotyl slightly are 1.77cm, and output ratio is 5.46.
After soya bean was cultivated and finishes: the root of bean sprouts was long for 5.23cm, hypocotyl length are that 14.77cm, hypocotyl slightly are 2.10cm, and output ratio is 5.29.
Embodiment 5, application seed sprouting device carry out germination test
1, the preparation of seed sprouting device
1.0g agar and 100mL water are mixed, and heating makes the agar dissolving, obtains agar solution.The step 1 of the other the same as in Example 2.
2, use the seed sprouting device and carry out germination test
Step 2 with embodiment 2.
After mung bean was cultivated and finished: the root of bean sprouts was long for 6.90cm, hypocotyl length are that 11.94cm, hypocotyl slightly are 1.74cm, and output ratio is 5.8.
After soya bean was cultivated and finishes, the root of bean sprouts was long for 3.90cm, hypocotyl length are that 10.48cm, hypocotyl slightly are 2.15cm, and output ratio is 4.12.
Embodiment 6, application seed sprouting device carry out germination test
1, the preparation of seed sprouting device
1.1g agar and 100mL water are mixed, and heating makes the agar dissolving, obtains agar solution.The step 1 of the other the same as in Example 2.
2, use the seed sprouting device and carry out germination test
Step 2 with embodiment 2.
After mung bean was cultivated and finished: the root of bean sprouts was long for 6.70cm, hypocotyl length are that 12.29cm, hypocotyl slightly are 1.79cm, and output ratio is 6.01.
After soya bean was cultivated and finishes: the root of bean sprouts was long for 3.93cm, hypocotyl length are that 11.60cm, hypocotyl slightly are 2.12cm, and output ratio is 4.45.
Claims (10)
1. seed sprouting device is solidified by aqueous agar solution and to obtain, and forms by base with perpendicular to some sidewalls of base, forms 1 groove between base and per two adjacent walls.
2. seed sprouting device as claimed in claim 1, it is characterized in that: the preparation method of described aqueous agar solution is as follows: 1.0-1.4g agar and 100mL water are mixed, obtain aqueous agar solution.
3. seed sprouting device as claimed in claim 1 or 2, it is characterized in that: the degree of depth of described groove is that 0.3-1.0cm, width are 0.3-0.5cm.
4. such as arbitrary described seed sprouting device in the claims 1 to 3, it is characterized in that: described base is rectangle.
5. seed sprouting device as claimed in claim 4, it is characterized in that: the length of each groove of described seed sprouting device is all consistent with the width of described base.
6. such as arbitrary described bean germination device in the claim 1 to 5, it is characterized in that: the degree of depth of each groove of described seed sprouting device and width equate.
7. method of cultivating seed sprouting is seed is placed the groove of the arbitrary described seed sprouting device of claim 1 to 6 and to cultivate.
8. method as claimed in claim 7, it is characterized in that: described seed is legume-seeds.
9. method as claimed in claim 8, it is characterized in that: the condition of described cultivation is: 20-25 ℃, 60%-95% humidity lucifuge were cultivated 4-6 days.
10. the application of arbitrary described method in detecting the bean germination proterties in arbitrary described seed sprouting device or the claim 7 to 9 in the claim 1 to 6.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210459369XA CN102939894A (en) | 2012-11-15 | 2012-11-15 | Seed germination device and method for detecting bean germination characters by same |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210459369XA CN102939894A (en) | 2012-11-15 | 2012-11-15 | Seed germination device and method for detecting bean germination characters by same |
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| CN102939894A true CN102939894A (en) | 2013-02-27 |
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| CN201210459369XA Pending CN102939894A (en) | 2012-11-15 | 2012-11-15 | Seed germination device and method for detecting bean germination characters by same |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201797719U (en) * | 2010-09-14 | 2011-04-20 | 中国农业科学院作物科学研究所 | Seed germination and vigor testing device |
| CN102342203A (en) * | 2010-07-24 | 2012-02-08 | 王泽军 | Dragnet-type sowing method for crops |
| CN102726147A (en) * | 2012-07-12 | 2012-10-17 | 贵州省烟草科学研究所 | Tobacco seed germinating bed and preparation method thereof |
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- 2012-11-15 CN CN201210459369XA patent/CN102939894A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102342203A (en) * | 2010-07-24 | 2012-02-08 | 王泽军 | Dragnet-type sowing method for crops |
| CN201797719U (en) * | 2010-09-14 | 2011-04-20 | 中国农业科学院作物科学研究所 | Seed germination and vigor testing device |
| CN102726147A (en) * | 2012-07-12 | 2012-10-17 | 贵州省烟草科学研究所 | Tobacco seed germinating bed and preparation method thereof |
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Application publication date: 20130227 |