CN214102277U

CN214102277U - Agar gel germinating bed

Info

Publication number: CN214102277U
Application number: CN202020679466.XU
Authority: CN
Inventors: 张向前; 路战远; 程玉臣; 张德健; 郭晓霞; 史宏飞; 咸丰; 陈立宇; 张建中; 赵小庆; 史功赋; 孙佩智; 任肖云; 方静; 张健伟; 杜香玉; 陈丽荣; 刘亚楠
Original assignee: Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences
Current assignee: Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences
Priority date: 2020-04-22
Filing date: 2020-04-22
Publication date: 2021-09-03
Anticipated expiration: 2030-04-22
Also published as: CN214385027U

Abstract

The utility model provides an agar gel germinating bed, which belongs to the technical field of seed germination, and takes agar gel as germinating bed matrix, a plurality of germinating holes are arranged on the agar gel, and the concentration of the agar gel is 0.3% -3%; the thickness of the agar gel is 1-1.5 cm, and the depth of the germination holes is 0.8-1.2 cm. The seed germination method has the advantages that the agar gel germination bed can enable wheat to germinate rapidly, the germination rate is high, and uniform and stable batch production of wheat seedlings can be realized.

Description

Agar gel germinating bed

Technical Field

The utility model belongs to the technical field of the seed germination, especially, relate to an agar gel germinating bed.

Background

The germination beds used in the traditional germination method mainly comprise three types, namely a paper germination bed, a sand germination bed and a soil germination bed, and the paper germination bed is mainly selected for wheat seed germination. However, in practice, paper beds have been found to suffer from a number of disadvantages. For example, a paper germination bed has poor water retention performance, and water needs to be constantly supplemented in the germination process of seeds in order to ensure that the seeds can normally germinate; if the water is supplemented too much at one time, the mixture is mixed into a whole, and the counting is difficult. Secondly, the paper germinating bed has poor adsorptivity and difficult dibbling, seeds are easy to roll in the dibbling process, and moreover, the paper germinating bed has poor supporting performance and is easy to damage, thus influencing the germinating effect.

SUMMERY OF THE UTILITY MODEL

In view of the above, the present invention is directed to an agar gel germinating bed; the seed germination bed can enable wheat to germinate rapidly, the germination rate is high, and uniform and stable batch production of wheat seedlings can be performed.

The utility model provides an agar gel germinating bed, which takes agar gel as germinating bed matrix, 40-60 germinating holes are arranged on the agar gel, and the concentration of the agar gel is 0.3% -3%; the thickness of the agar gel is 1-1.5 cm, and the depth of the germination holes is 0.8-1.2 cm.

Preferably, the diameter of the germinating hole is 0.3-0.4 cm.

Preferably, when the seeds to be germinated are naked seeds, the concentration of the agar gel is 1.5-2.1%.

Preferably, when the seeds to be germinated are naked seeds, the concentration of the agar gel is 0.3-1.5%.

The utility model has the advantages that: the agar gel germinating bed provided by the utility model takes the agar gel as the substrate, not only solves the problem that the paper germinating bed needs frequent water replenishing, but also is easier to sow and count compared with the paper germinating bed; the wheat seeds can be fixed in the germination holes, water is not required to be supplemented, and the problem of seed accumulation caused by water addition is avoided; agar gel germinating bed can make wheat sprout fast, and the germination percentage is high, can carry out even stable wheat seedling batch production. The preparation method of the agar gel germinating bed provided by the utility model is simple and easy to operate, can greatly reduce the labor and has low cost.

Drawings

FIG. 1 is a schematic structural diagram of a wheat agar gel germination puncher;

FIG. 2 is a schematic diagram of the structure of an agar germinating bed.

Detailed Description

The utility model discloses in, the diameter of germinating hole is preferably 0.3 ~ 0.4 cm. In the utility model, the agar gel germinating bed is preferably fixed in the container, the utility model has no special requirements for the shape and the material of the container, and the conventional container in the field is adopted. In the specific implementation process of the utility model, the container can be selected as a culture dish; the diameter of the culture dish is preferably 10 cm.

In the present invention, when the seeds to be germinated are naked seeds, the concentration of the agar gel is preferably 1.5% to 3%, more preferably 1.5% to 2.1%. When the seeds to be germinated are coated seeds, the concentration of the agar gel is preferably 0.3 to 1.5%, more preferably 0.4 to 0.9%. In the present invention, the concentration of the agar gel specifically refers to the gram amount of agar powder used per 100ml of buffer solution; when the concentration of the agar gel is 0.3%, it means that 0.3g of agar powder is contained in 100ml of the buffer solution; in the present invention, the buffer is preferably a tap water and agar gel melting buffer.

The utility model provides a hole making device, which comprises a bottom plate and a plurality of hole making columns fixedly connected to the bottom plate, wherein the hole making columns are vertical to the bottom plate; the diameter of the hole making column is 0.3-0.4 cm, and the height of the hole making column is 0.8-1.2 cm; the distance between two adjacent hole making columns is 1.0-1.2 cm.

The utility model discloses it is right the shape and the material of bottom plate do not have special restrictions, adopt the conventional shape in this field and material all can. In the present invention, the shape of the bottom plate is preferably identical to the shape of the agar gel germination bed. In the utility model discloses in, work as bottom plate and the preferred wooden material that adopts of system hole post.

Utilize agar gel germinating bed and hole puncher carry out seed germination method, including following step: 1) injecting the agar solution into a container after hot melting, wherein the thickness of the agar solution is 1-1.5 cm; 2) vertically inserting the hole making device into the agar solution, and pulling out the hole making device after the agar solution is solidified to obtain an agar gel germination bed; 3) and placing the seeds in the germinating holes for culturing.

The utility model discloses in, pour into the container into after the agar solution hot melt, agar solution's thickness is 1 ~ 1.5 cm. The utility model discloses in, the temperature of agar solution hot melt is preferably 110 ~ 125 ℃, in the utility model discloses in, agar solution's thickness is preferably 1.1 ~ 1.4 cm.

The utility model discloses in, will the hole making ware insert perpendicularly in the agar-agar solution, extract after waiting the agar-agar solution to solidify and obtain the agar-agar gel germinating bed. In the present invention, the perforator is preferably inserted immediately after the agar solution is injected into the container.

The utility model discloses in, arrange the seed in the germination hole and cultivate. In the present invention, the seeds are preferably wheat seeds; preferably, one seed is placed in each germination hole. In the utility model, the temperature of cultivation is 27 ~ 29 ℃, more preferably 28 ℃, the humidity of cultivation is preferred to be more than or equal to 40%. In the utility model, the cultivation is preferably carried out in a constant temperature incubator, and the illumination condition in the cultivation process is 12h illumination-12 h darkness which are carried out alternately. The utility model discloses the preferred moisturizing that does not carry out of in-process of cultivateing.

The technical solutions provided by the present invention are described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of the present invention.

The wheat seeds in the following examples are all provided by crops of the academy of sciences of the inner Mongolia farming-animal husbandry.

Agar powder produced by Shanghai Gaokeng company with gel strength of 900g/cm²The total ash content is less than or equal to 6.5 percent, the heavy metal content is less than 40ppm, and the lead content is less than 10 ppm.

The seed cultivation temperature is 28 ℃, the relative humidity is more than 40%, and the seeds are cultivated in an artificial climate incubator (RZH850A) with 12h alternating illumination and darkness, and the length of radicle and seeds is taken as the germination standard.

The germination of naked seeds is strictly performed according to the national standard GB/T17317-1998, and the coating of seeds is performed according to GB 15671-1995.

The germination potential is the percentage of the number of the germinated seeds in the seed number of the tested variety when the number of the germinated seeds reaches the maximum peak in the germination process.

The germination percentage is the percentage of the number of normal germinated seeds to the number of test seeds within a predetermined number of days (at the time of initial counting) at the initial stage of the germination test.

Analysis of variance was performed using SPSS16.0 and the results were the average of four replicates.

The culture dish has a diameter of 10cm at the bottom, a height of 2.0cm and a thickness of 0.2cm at the bottom.

Example 1

1. Preparation of a puncher: on a square wood board with the side length of 8cm, 7 rows of cylinders with the diameter of 0.3cm and the length of more than 1.0cm are fixed, except 8 holes in the middle row, 7 cylinders are fixed in each row, and the maximum distance between every two adjacent rows and columns is 1.2 cm.

2. Dissolving agar powder by adopting an SX-500 autoclave to obtain agar solution with required concentration, injecting the obtained agar solution into the culture dish in the step 2 to ensure that the thickness of the liquid agar is more than 1.0cm, then immediately downwards inserting the cylinder on the puncher into the culture dish, after solidification, slightly and vertically pulling out the puncher, and leaving 50 seeding holes on an agar bed.

Preparing agar gel germination beds with agar gel concentrations of 0.3%, 0.6%, 0.9%, 1.2%, 1.5%, 1.8%, 2.1%, 2.4%, 2.7%, and 3.0% according to the above method; 3 parts of each concentration is prepared, and then, naked seeds of Nomame No. 2, Nomame No. 4, Yongliang No. 4, Ningchun 50 and Longmai 33 and coated seeds of Nomame No. 2 are sown in the holes on the agar bed. The following operations are carried out:

firstly, spreading 2 pieces of filter paper in a culture dish, and wetting the filter paper by distilled water to obtain a paper bed as a control, wherein the number and the spacing of dibbled seeds are the same as those of an agar bed.

And (3) counting the germination rate, the germination potential and the germination index of the seeds in the germination bed, wherein the specific data are shown in tables 1, 2 and 3.

TABLE 1 comparison of the germination rates of naked and coated wheat seeds No. 2 of Nongmai on agar and paper beds

As can be seen from Table 1, the naked and coated wheat seeds of NommaMc2 have obvious difference in germination rate on agar bed and paper bed, and the concentration of agar germination bed has obvious influence on the germination rate of seeds. On a paper bed, the germination rate of the naked seeds is 92 percent and is obviously higher than the germination rate of the coated seeds by 79 percent. The germination rates of the wheat naked seeds and the coated seeds with different agar concentrations are different on the agar bed. The germination rates of the naked wheat seeds and the coated wheat seeds reach the maximum values by the fourth day when the agar concentration is 0.60 percent, and are respectively 97 percent and 95 percent.

TABLE 2 comparison of the germination vigor of different wheat varieties on agar and paper beds

As can be seen from Table 2, different wheat varieties have distinct differences in germination vigor on the agar bed and the paper bed, and the concentration of the agar bed also has an influence on the germination vigor of wheat seeds. When the concentration of the wheat variety in an agar bed is 0.3%, the germination potential reaches the maximum value, wherein the germination potential of Ningchun 50 is 99% at most, and the germination potential of Longmai 33 is 88% at least.

TABLE 3 comparison of germination indices of different wheat varieties on agar and paper beds

As can be seen from Table 3, different wheat varieties have distinct differences in germination potential on the agar bed and the paper bed, and the concentration of the agar bed also has an effect on the germination index of wheat seeds.

According to the above embodiment, the utility model provides an agar gel germinating bed uses agar gel as the matrix, has not only solved the problem that paper germinating bed need the moisturizing frequently, and for the easier seeding of paper bed, statistics, the wheat germination percentage is high.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims

1. The agar gel germination bed is characterized in that agar gel is used as a germination bed matrix, and 40-60 germination holes are formed in the agar gel; the thickness of the agar gel is 1-1.5 cm, and the depth of the germination holes is 0.8-1.2 cm.

2. The agar gel germination bed according to claim 1, wherein the diameter of the germination pores is 0.3-0.4 cm.