CN109463221A - The method for screening drought-enduring cabbage type rape variety - Google Patents
The method for screening drought-enduring cabbage type rape variety Download PDFInfo
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- CN109463221A CN109463221A CN201811609216.2A CN201811609216A CN109463221A CN 109463221 A CN109463221 A CN 109463221A CN 201811609216 A CN201811609216 A CN 201811609216A CN 109463221 A CN109463221 A CN 109463221A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/15—Leaf crops, e.g. lettuce or spinach
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/25—Dry fruit hulls or husks, e.g. chaff or coir
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/30—Growth substrates; Culture media; Apparatus or methods therefor based on or containing synthetic organic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
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Abstract
The invention discloses a kind of methods for screening drought-enduring cabbage type rape variety, comprising steps of selected full seed, uniformity, disease-free cabbage type rape seed disinfection, sow later, after planting covered with matrix；Obtained cabbage type rape seedling is divided into 2 groups of Osmotic treatment group, 1 group of control and control；Osmotic treatment group is planted with organic ecological cultivation matrix, it is to be grown to 41 heart of leaf when carry out Osmotic treatment, the relative humidity staged of matrix is down to 30%, and maintains arid；It compares 1 group to plant using organic ecological cultivation matrix, the relative humidity of matrix is maintained 75% always；It compares 2 groups to plant using soil matrix, the relative humidity of matrix is maintained 75% always；The underground part fresh weight of Osmotic treatment group, every plant of cabbage type rape for compareing 1 group and compareing 2 groups is counted respectively；Calculate each kind drought-resistant ability value.This method is at low cost, and drought-enduring accuracy in detection is high, and detection cycle is short, easily operated, convenient in each local promotion and implementation.
Description
Technical field
The present invention relates to plant variety screening technique fields, and in particular to a kind of side for screening drought-enduring cabbage type rape variety
Method.
Background technique
Cabbage type rape, one of rape three categories type, originating from Europe, at present with Europe various countries, portion by north and Canada west
The part cloth by north of portion's grassland ecological is more.China is introduced the 1930s by Korea, Japan and Britain, widely distributed
In all over China, concentrated the most with the distribution of Yangtze river basin each province rape main producing region especially.In addition, cabbage type rape plant has centainly
Salt tolerance, be China coast beach and inland salt-soda soil development and utilization important plant resources.But due to cabbage type rape kind
Sub- germination period and seedling stage are more sensitive to salt stress, and the sub- sprouting of sowing of middle high salinity is suppressed, therefore in the salt of salinity unevenness
The phenomenon that being usually short of seedling when the commerial growing of alkali ground.The salt tolerance for improving cabbage type rape germination period and seedling stage, for
Realize that cabbage type rape is of great significance in the exploitation of the culture and utility and saline and alkaline soil in saline and alkaline beach area.Therefore, it improves
The drought resistance of crop varieties has become the key subjects of arid and semi-arid area agricultural research；And accurately have both crop
The drought resistance of kind is the necessary basis for cultivating drought-resistant variety.Drought resisting identification be exactly by crop varieties drought-resistant ability size into
Row screening, evaluation and the process sorted out；High-quality germplasm can be provided for Drought-resistant Breeding by being identified by drought resisting.Although different researchers
Work is studied to the drought resisting of cabbage type rape from many aspects, has carried out beneficial spy for understanding cabbage type rape drought resisting
It begs for, but the screening of existing drought-enduring cabbage type rape variety has accurate low disadvantage, and it is easy to control, easy to lack a kind of planting environment
The method of operation and the high drought-enduring cabbage type rape variety of accuracy.
Summary of the invention
For the defects in the prior art, the present invention provides a kind of method for screening drought-enduring cabbage type rape variety, the sieve
Choosing method is at low cost, and drought-enduring accuracy in detection is high, and detection cycle is short, easily operated, convenient in each local promotion and implementation.
To achieve the above object, technical solution provided by the invention are as follows:
The present invention provides a kind of methods for screening drought-enduring cabbage type rape variety, comprising steps of step S1, selected seed
Full, uniformity, disease-free cabbage type rape seed carry out disinfection, later with broadcast mode sowing in cultivation bed, after planting
It is covered with matrix；Thinning is carried out when cabbage type rape seedling grows to 2 true leaves, chooses the consistent strong seedling of growing way, each cultivation
Slot stays 30 plants of cabbage type rape seedlings；Wherein, matrix is organic ecological cultivation matrix or soil matrix；The relative humidity of matrix controls
It is 75%；Cabbage type rape seedling is divided into 2 groups of Osmotic treatment group, 1 group of control and control by step S2；Osmotic treatment group, which uses, to be had
The plantation of machine ecological cultivation matrix, carries out Osmotic treatment, the relative humidity rank of matrix when cabbage type rape seedling grows to 41 heart of leaf
Ladder type is down to 30%, and maintains several days of arid；It compares 1 group to plant using organic ecological cultivation matrix, the relative humidity of matrix
Always it is maintained 75%；It compares 2 groups to plant using soil matrix, the relative humidity of matrix is maintained 75% always；Step S3, point
Not Tong Ji Osmotic treatment group, 1 group of control and compare 2 groups every plant of cabbage type rape underground part fresh weight, 30 in single cultivation bed
The average value of strain cabbage type rape underground part fresh weight is the cultivation bed cabbage type rape underground part fresh weight, and every group corresponding multiple
The average value of the cabbage type rape underground part fresh weight of cultivation bed is the cabbage type rape underground part fresh weight under the processing；Step
S4 calculates each cabbage type rape variety drought-resistant ability value, and drought-resistant ability value is bigger, illustrates corresponding cabbage type rape variety drought resisting
Ability is stronger；Single cabbage type rape variety drought-resistant ability value=(the kind Osmotic treatment group is with respect to underground part fresh weight/all mirror
Determine average value of the Osmotic treatment group with respect to underground part fresh weight of kind) × (the kind Osmotic treatment group is with respect to underground part fresh weight/and it should
1 group of varietal check opposite underground part fresh weight)；Wherein, Osmotic treatment group is fresh with respect to underground part fresh weight=Osmotic treatment group underground part
It is fresh to compare 1 group of opposite underground part fresh weight=1 group of control underground part fresh weight/2 groups of control underground part for 2 groups of underground part fresh weights of weight/control
Weight.It should be noted that for the accuracy of drought-resistant ability identification, single cabbage type rape variety drought-resistant ability value calculate when
It waits, background interference caused by cultivation matrix is excluded using relative value.
Preferably, in step S1, the raw material components of organic ecological cultivation matrix include corn stover, decomposed cow dung, rice husk,
Clinker and wormcast；Organic ecological cultivation matrix is decomposed using primary fermentation.
It is further preferred that in step S1, corn stover, decomposed cow dung, rice husk, clinker and wormcast mass ratio be 2:
4:1:3:3.
Preferably, in step S1, added with 1g carbendazim in every 10L organic ecological cultivation matrix.
Preferably, in step S1, the physicochemical property of organic ecological cultivation matrix includes: that bulk density is 0.71g/cm3, total pore space
Degree is 78%, ventilating slit 16%, and water holding hole is 62%, pH value 7.1, conductivity 3.68ms/cm, cation exchange
Amount is 62cmol/kg.
Preferably, entire screening process carries out in intelligent greenhouse, and greenhouse air relative humidity remains 70%, and temperature is
20℃。
Preferably, in step S1, a length of 100cm, width 50cm, the depth 30cm of cultivation bed.
Preferably, in step S1, the cladding thickness of matrix is 1.5~2.5cm, preferably 2cm.
Preferably, in step S2,2 groups of Osmotic treatment group, 1 group of control and control respectively include three cultivation beds.
Preferably, in step S2, several days are 40 days.
Technical solution provided by the invention, have it is following the utility model has the advantages that
(1) for plant, the development degree of underground root system determines the power of drought-resistant ability；Applicant intends base area
The development degree of lower root system identifies the drought resistance of kindred plant different cultivars, but the development degree of root system by matrix influenced compared with
Greatly.Cultivation matrix is more suitable for the growth of root system of plant for soil, for example cultivation matrix total porosity compares soil matrix
Greatly.For the growing environment built to root system, it is necessary first to develop and a be suitble to the cultivation of plants matrix.The present invention passes through
Study influence of the different formulations matrix to cabbage type rape growth potential, plant Photosynthetic Index, root system, yield and quality, it is determined that
The most adaptation side of cabbage type rape organic ecological cultivation matrix uses the cultivation matrix one side plant compared with field planting
Growing way is good, and yield and quality is promoted, and on the other hand can reduce the use of chemical fertilizer, pesticide, water-saving fertilizer-saving.
(2) studies have shown that the drought resistance of different cabbage type rape varieties is closely related with underground part fresh weight；And the present invention mentions
The method of the drought-enduring cabbage type rape variety of screening of confession has at low cost, and drought-enduring accuracy in detection is high, and detection cycle is short, is easy to grasp
The advantages that making, convenient in each local promotion and implementation.
Additional aspect and advantage of the invention will be set forth in part in the description, and will partially become from the following description
Obviously, or practice through the invention is recognized.
Specific embodiment
Below in conjunction with the embodiment of the present invention, technical solution of the present invention is clearly and completely described.Implement below
Example is only used for clearly illustrating technical solution of the present invention, therefore is intended only as example, and cannot be used as a limitation and limit this hair
Bright protection scope.
Experimental method in following embodiments is unless otherwise specified conventional method.Examination as used in the following examples
Material is tested, is to be commercially available from regular shops unless otherwise specified.Quantitative test in following embodiment, is respectively provided with three
Secondary to repeat to test, data are the average value or mean+SD of three repeated experiments.
Embodiment
The present embodiment provides a kind of methods for screening drought-enduring cabbage type rape variety, and entire screening process is in intelligent greenhouse
It carries out, greenhouse air relative humidity remains 70%, and it is 20 DEG C that temperature, which is 20 DEG C, and screening specifically includes step:
Step S1 (cabbage type rape sowing): selected full seed, uniformity, disease-free cabbage type rape seed disappear
Poison is sowed later in the cultivation bed that a length of 100cm, width 50cm, depth are 30cm, with a broadcast mode plantation, keeps reasonable strain
Away from；After planting 2cm is covered with matrix；
Thinning is carried out when cabbage type rape seedling grows to 2 true leaves, chooses the consistent strong seedling of growing way, is keeping reasonable strain
Away from the basis of, each cultivation bed stays 30 plants of cabbage type rape seedlings；Wherein, matrix is organic ecological cultivation matrix or soil base
Matter；From sowing until before Osmotic treatment, the relative humidity control of the matrix of all cultivation beds is 75%；Organic ecological cultivation base
The raw material components of matter include crushing maize stalk, decomposed cow dung, rice husk, clinker and the wormcast that mass ratio is 2:4:1:3:3,
Added with 1g carbendazim in every 10L organic ecological cultivation matrix, organic ecological cultivation matrix is decomposed using primary fermentation；Ecological organic
It is tamped when cultivation matrix uses without pressing deliberately, the physicochemical property of the organic ecological cultivation matrix is as follows: bulk density is
0.71g/cm3, total porosity 78%, ventilating slit 16%, water holding hole is 62%, pH value 7.1, and conductivity is
3.68ms/cm, cation exchange capacity (CEC) 62cmol/kg.
Step S2 (Osmotic treatment): cabbage type rape seedling is divided into 2 groups of Osmotic treatment group, 1 group of control and control；Each place
3 cultivation beds are managed, every slot stays 30 plants of cabbage type rape seedlings after thinning:
Osmotic treatment group (cultivation matrix Osmotic treatment): it is planted using organic ecological cultivation matrix, to cabbage type rape seedling
Osmotic treatment is carried out when growing to 41 heart of leaf, staged is down to 30% to the relative humidity of matrix within a certain period of time, and remains dry
Drought 40 days；
It compares 1 group (cultivation matrix water content is normal): being planted using organic ecological cultivation matrix, the relative humidity of matrix begins
It is maintained 75% eventually；
It compares 2 groups (soil matrix water content is normal): being planted using soil matrix, the relative humidity of matrix is maintained always
75%.
Step S3 (data statistics): Osmotic treatment group, 1 group of control and every plant of cabbage type rape for compareing 2 groups are counted respectively
Underground part fresh weight, every plant of meter averages three times, the underground part fresh weight of as this plant cabbage type rape；
The average value of 30 plants of cabbage type rape underground part fresh weights is the cultivation bed cabbage type rape in single cultivation bed
Lower part fresh weight, the average value of the cabbage type rape underground part fresh weight of every group of corresponding three cultivation bed are the Wild cabbage type oil of the group
Vegetable plot lower part fresh weight.
Step S4 (drought-resistant ability value calculates and drought-resistance ability identification): each cabbage type rape variety drought-resistant ability is calculated
Value, drought-resistant ability value is bigger, illustrates that corresponding cabbage type rape variety drought-resistant ability is stronger；
Single cabbage type rape variety drought-resistant ability value=(the kind Osmotic treatment group is with respect to underground part fresh weight/all mirror
Determine average value of the Osmotic treatment group with respect to underground part fresh weight of kind) × (the kind Osmotic treatment group is with respect to underground part fresh weight/and it should
1 group of varietal check opposite underground part fresh weight)；
Wherein, Osmotic treatment group is fresh with respect to underground part fresh weight=Osmotic treatment group underground part fresh weight/2 groups of control underground part
Weight；
Compare 1 group of opposite underground part fresh weight=1 group of control underground part fresh weight/2 groups of control underground part fresh weight.
Comparative example 1
This comparative example provides a kind of method for screening drought-enduring cabbage type rape variety, and entire screening process is in intelligent greenhouse
It carries out, greenhouse air relative humidity remains 70%, and it is 20 DEG C that temperature, which is 20 DEG C, and screening specifically includes step:
Step S1 (cabbage type rape sowing): selected full seed, uniformity, disease-free cabbage type rape seed disappear
Poison is sowed later in the cultivation bed that a length of 100cm, width 50cm, depth are 30cm, with a broadcast mode plantation, keeps reasonable strain
Away from；After planting 2cm is covered with matrix；
Thinning is carried out when cabbage type rape seedling grows to 2 true leaves, chooses the consistent strong seedling of growing way, is keeping reasonable strain
Away from the basis of, each cultivation bed stays 30 plants of cabbage type rape seedlings；Wherein, matrix is organic ecological cultivation matrix or soil base
Matter；From sowing until before Osmotic treatment, the relative humidity control of the matrix of all cultivation beds is 75%；Organic ecological cultivation base
The raw material components of matter include crushing maize stalk, decomposed cow dung, rice husk, clinker and the wormcast that mass ratio is 4:4:1:3:3,
Added with 1g carbendazim in every 10L organic ecological cultivation matrix, organic ecological cultivation matrix is decomposed using primary fermentation；Ecological organic
It is tamped when cultivation matrix uses without pressing deliberately.
Step S2 (Osmotic treatment): cabbage type rape seedling is divided into 2 groups of Osmotic treatment group, 1 group of control and control；Each place
3 cultivation beds are managed, every slot stays 30 plants of cabbage type rape seedlings after thinning:
Osmotic treatment group (cultivation matrix Osmotic treatment): it is planted using organic ecological cultivation matrix, to cabbage type rape seedling
Osmotic treatment is carried out when growing to 41 heart of leaf, staged is down to 30% to the relative humidity of matrix within a certain period of time, and remains dry
Drought 40 days；
It compares 1 group (cultivation matrix water content is normal): being planted using organic ecological cultivation matrix, the relative humidity of matrix begins
It is maintained 75% eventually；
It compares 2 groups (soil matrix water content is normal): being planted using soil matrix, the relative humidity of matrix is maintained always
75%.
Step S3 (data statistics): Osmotic treatment group, 1 group of control and every plant of cabbage type rape for compareing 2 groups are counted respectively
Underground part fresh weight, every plant of meter averages three times, the underground part fresh weight of as this plant cabbage type rape；
The average value of 30 plants of cabbage type rape underground part fresh weights is the cultivation bed cabbage type rape in single cultivation bed
Lower part fresh weight, the average value of the cabbage type rape underground part fresh weight of every group of corresponding three cultivation bed are the Wild cabbage type oil of the group
Vegetable plot lower part fresh weight.
Step S4 (drought-resistant ability value calculates and drought-resistance ability identification): each cabbage type rape variety drought-resistant ability is calculated
Value, drought-resistant ability value is bigger, illustrates that corresponding cabbage type rape variety drought-resistant ability is stronger；
Single cabbage type rape variety drought-resistant ability value=(the kind Osmotic treatment group is with respect to underground part fresh weight/all mirror
Determine average value of the Osmotic treatment group with respect to underground part fresh weight of kind) × (the kind Osmotic treatment group is with respect to underground part fresh weight/and it should
1 group of varietal check opposite underground part fresh weight)；
Wherein, Osmotic treatment group is fresh with respect to underground part fresh weight=Osmotic treatment group underground part fresh weight/2 groups of control underground part
Weight；
Compare 1 group of opposite underground part fresh weight=1 group of control underground part fresh weight/2 groups of control underground part fresh weight.
Comparative example 2
This comparative example provides a kind of method for screening drought-enduring cabbage type rape variety, and entire screening process is in intelligent greenhouse
It carries out, greenhouse air relative humidity remains 70%, and it is 20 DEG C that temperature, which is 20 DEG C, and screening specifically includes step:
Step S1 (cabbage type rape sowing): selected full seed, uniformity, disease-free cabbage type rape seed disappear
Poison is sowed later in the cultivation bed that a length of 100cm, width 50cm, depth are 30cm, with a broadcast mode plantation, keeps reasonable strain
Away from；After planting 2cm is covered with matrix；
Thinning is carried out when cabbage type rape seedling grows to 2 true leaves, chooses the consistent strong seedling of growing way, is keeping reasonable strain
Away from the basis of, each cultivation bed stays 30 plants of cabbage type rape seedlings；Wherein, matrix is organic ecological cultivation matrix or soil base
Matter；From sowing until before Osmotic treatment, the relative humidity control of the matrix of all cultivation beds is 75%；Organic ecological cultivation base
The raw material components of matter include decomposed cow dung, rice husk, clinker and the wormcast that mass ratio is 4:1:3:3, are planted in every 10L ecological organic
It trains added with 1g carbendazim in matrix, organic ecological cultivation matrix is decomposed using primary fermentation；When organic ecological cultivation matrix uses
Without pressing compacting deliberately.
Step S2 (Osmotic treatment): cabbage type rape seedling is divided into 2 groups of Osmotic treatment group, 1 group of control and control；Each place
3 cultivation beds are managed, every slot stays 30 plants of cabbage type rape seedlings after thinning:
Osmotic treatment group (cultivation matrix Osmotic treatment): it is planted using organic ecological cultivation matrix, to cabbage type rape seedling
Osmotic treatment is carried out when growing to 41 heart of leaf, staged is down to 30% to the relative humidity of matrix within a certain period of time, and remains dry
Drought 40 days；
It compares 1 group (cultivation matrix water content is normal): being planted using organic ecological cultivation matrix, the relative humidity of matrix begins
It is maintained 75% eventually；
It compares 2 groups (soil matrix water content is normal): being planted using soil matrix, the relative humidity of matrix is maintained always
75%.
Step S3 (data statistics): Osmotic treatment group, 1 group of control and every plant of cabbage type rape for compareing 2 groups are counted respectively
Underground part fresh weight, every plant of meter averages three times, the underground part fresh weight of as this plant cabbage type rape；
The average value of 30 plants of cabbage type rape underground part fresh weights is the cultivation bed cabbage type rape in single cultivation bed
Lower part fresh weight, the average value of the cabbage type rape underground part fresh weight of every group of corresponding three cultivation bed are the Wild cabbage type oil of the group
Vegetable plot lower part fresh weight.
Step S4 (drought-resistant ability value calculates and drought-resistance ability identification): each cabbage type rape variety drought-resistant ability is calculated
Value, drought-resistant ability value is bigger, illustrates that corresponding cabbage type rape variety drought-resistant ability is stronger；
Single cabbage type rape variety drought-resistant ability value=(the kind Osmotic treatment group is with respect to underground part fresh weight/all mirror
Determine average value of the Osmotic treatment group with respect to underground part fresh weight of kind) × (the kind Osmotic treatment group is with respect to underground part fresh weight/and it should
1 group of varietal check opposite underground part fresh weight)；
Wherein, Osmotic treatment group is fresh with respect to underground part fresh weight=Osmotic treatment group underground part fresh weight/2 groups of control underground part
Weight；
Compare 1 group of opposite underground part fresh weight=1 group of control underground part fresh weight/2 groups of control underground part fresh weight.
Comparative example 3
This comparative example provides a kind of method for screening drought-enduring cabbage type rape variety, and entire screening process is in intelligent greenhouse
It carries out, greenhouse air relative humidity remains 70%, and it is 20 DEG C that temperature, which is 20 DEG C, and screening specifically includes step:
Step S1 (cabbage type rape sowing): selected full seed, uniformity, disease-free cabbage type rape seed disappear
Poison is sowed later in the cultivation bed that a length of 100cm, width 50cm, depth are 30cm, with a broadcast mode plantation, keeps reasonable strain
Away from；After planting 2cm is covered with matrix；
Thinning is carried out when cabbage type rape seedling grows to 2 true leaves, chooses the consistent strong seedling of growing way, is keeping reasonable strain
Away from the basis of, each cultivation bed stays 30 plants of cabbage type rape seedlings；Wherein, matrix is organic ecological cultivation matrix or soil base
Matter；From sowing until before Osmotic treatment, the relative humidity control of the matrix of all cultivation beds is 75%；Organic ecological cultivation base
The raw material components of matter include crushing maize stalk, decomposed cow dung, rice husk and the clinker that mass ratio is 2:4:1:3, organic in every 10L
Added with 1g carbendazim in ecological cultivation matrix, organic ecological cultivation matrix is decomposed using primary fermentation；Organic ecological cultivation matrix
It is tamped when use without pressing deliberately.
Step S2 (Osmotic treatment): cabbage type rape seedling is divided into 2 groups of Osmotic treatment group, 1 group of control and control；Each place
3 cultivation beds are managed, every slot stays 30 plants of cabbage type rape seedlings after thinning:
Osmotic treatment group (cultivation matrix Osmotic treatment): it is planted using organic ecological cultivation matrix, to cabbage type rape seedling
Osmotic treatment is carried out when growing to 41 heart of leaf, staged is down to 30% to the relative humidity of matrix within a certain period of time, and remains dry
Drought 40 days；
It compares 1 group (cultivation matrix water content is normal): being planted using organic ecological cultivation matrix, the relative humidity of matrix begins
It is maintained 75% eventually；
It compares 2 groups (soil matrix water content is normal): being planted using soil matrix, the relative humidity of matrix is maintained always
75%.
Step S3 (data statistics): Osmotic treatment group, 1 group of control and every plant of cabbage type rape for compareing 2 groups are counted respectively
Underground part fresh weight, every plant of meter averages three times, the underground part fresh weight of as this plant cabbage type rape；
The average value of 30 plants of cabbage type rape underground part fresh weights is the cultivation bed cabbage type rape in single cultivation bed
Lower part fresh weight, the average value of the cabbage type rape underground part fresh weight of every group of corresponding three cultivation bed are the Wild cabbage type oil of the group
Vegetable plot lower part fresh weight.
Step S4 (drought-resistant ability value calculates and drought-resistance ability identification): each cabbage type rape variety drought-resistant ability is calculated
Value, drought-resistant ability value is bigger, illustrates that corresponding cabbage type rape variety drought-resistant ability is stronger；
Single cabbage type rape variety drought-resistant ability value=(the kind Osmotic treatment group is with respect to underground part fresh weight/all mirror
Determine average value of the Osmotic treatment group with respect to underground part fresh weight of kind) × (the kind Osmotic treatment group is with respect to underground part fresh weight/and it should
1 group of varietal check opposite underground part fresh weight)；
Wherein, Osmotic treatment group is fresh with respect to underground part fresh weight=Osmotic treatment group underground part fresh weight/2 groups of control underground part
Weight；
Compare 1 group of opposite underground part fresh weight=1 group of control underground part fresh weight/2 groups of control underground part fresh weight.
The cabbage type rape underground part fresh weight growing state of the observation embodiment of the present invention, comparative example 1 to comparative example 3, and in
The cabbage type rape underground part fresh weight that conventional screening assays obtain compares, and concrete outcome is as shown in table 1 below.
Table 1 is different to organize other cabbage type rape underground part fresh weight growing state
It should be noted that unless otherwise indicated, technical term or scientific term used in this application should be this hair
The ordinary meaning that bright one of ordinary skill in the art are understood.Unless specifically stated otherwise, it otherwise illustrates in these embodiments
Component and opposite step, numerical expression and the numerical value of step are not limit the scope of the invention.It is illustrated and described herein
In all examples, unless otherwise prescribed, any occurrence should be construed as merely illustratively, not as limitation, because
This, other examples of exemplary embodiment can have different values.
In the description of the present invention, it is to be understood that, term " first ", " second " are used for description purposes only, and cannot
It is interpreted as indication or suggestion relative importance or implicitly indicates the quantity of indicated technical characteristic.Define as a result, " the
One ", the feature of " second " can explicitly or implicitly include one or more of the features.In the description of the present invention,
The meaning of " plurality " is two or more, unless otherwise specifically defined.
Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention., rather than its limitations；To the greatest extent
Pipe present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that: its according to
So be possible to modify the technical solutions described in the foregoing embodiments, or to some or all of the technical features into
Row equivalent replacement；And these are modified or replaceed, various embodiments of the present invention technology that it does not separate the essence of the corresponding technical solution
The range of scheme should all cover within the scope of the claims and the description of the invention.
Claims (10)
1. a kind of method for screening drought-enduring cabbage type rape variety, which is characterized in that comprising steps of
Step S1, selected full seed, uniformity, disease-free cabbage type rape seed are carried out disinfection, are broadcast later with a broadcast mode
Kind is after planting covered with matrix in cultivation bed；Thinning is carried out when cabbage type rape seedling grows to 2 true leaves, chooses growing way
Consistent strong seedling, each cultivation bed stay 30 plants of cabbage type rape seedlings；Wherein, the matrix is organic ecological cultivation matrix or soil
Matrix；The relative humidity control of matrix is 75%；
Cabbage type rape seedling is divided into 2 groups of Osmotic treatment group, 1 group of control and control by step S2；Osmotic treatment group uses organic life
The plantation of state cultivation matrix, carries out Osmotic treatment, the relative humidity staged of matrix when cabbage type rape seedling grows to 41 heart of leaf
It is down to 30%, and maintains several days of arid；It compares 1 group to plant using organic ecological cultivation matrix, the relative humidity of matrix is always
It is maintained 75%；It compares 2 groups to plant using soil matrix, the relative humidity of matrix is maintained 75% always；
Step S3 counts the underground part fresh weight of Osmotic treatment group, every plant of cabbage type rape for compareing 1 group and compareing 2 groups respectively, single
The average value of 30 plants of cabbage type rape underground part fresh weights is the cultivation bed cabbage type rape underground part fresh weight in a cultivation bed, often
The average value of the cabbage type rape underground part fresh weight of the corresponding multiple cultivation beds of group is the cabbage type rape underground under the processing
Portion's fresh weight；
Step S4 calculates each cabbage type rape variety drought-resistant ability value, and drought-resistant ability value is bigger, illustrates corresponding cabbage type rape
Kind drought-resistant ability is stronger；Single cabbage type rape variety drought-resistant ability value=(the kind Osmotic treatment group is fresh with respect to underground part
Average value of weight/all identification of species Osmotic treatment group with respect to underground part fresh weight) × (the kind Osmotic treatment group relatively under
Portion's fresh weight/1 group of varietal check is with respect to underground part fresh weight)；Wherein, Osmotic treatment group is with respect to underground part fresh weight=Osmotic treatment group
Underground part fresh weight/2 groups of control underground part fresh weight compares 1 group of opposite underground part fresh weight=1 group of control underground part fresh weight/2 groups of control
Underground part fresh weight.
2. the method for the drought-enduring cabbage type rape variety of screening according to claim 1, it is characterised in that:
In the step S1, the raw material components of the organic ecological cultivation matrix include corn stover, decomposed cow dung, rice husk, furnace
Slag and wormcast；The organic ecological cultivation matrix is decomposed using primary fermentation.
3. the method for the drought-enduring cabbage type rape variety of screening according to claim 2, it is characterised in that:
In the step S1, the matter of the corn stover, the decomposed cow dung, the rice husk, the clinker and the wormcast
Amount is than being 2:4:1:3:3.
4. the method for the drought-enduring cabbage type rape variety of screening according to claim 1, it is characterised in that:
In the step S1, added with 1g carbendazim in every 10L organic ecological cultivation matrix.
5. the method for the drought-enduring cabbage type rape variety of screening according to claim 1, it is characterised in that:
In the step S1, the physicochemical property of the organic ecological cultivation matrix includes: that bulk density is 0.71g/cm3, total porosity is
78%, ventilating slit 16%, water holding hole is 62%, pH value 7.1, conductivity 3.68ms/cm, and cation exchange capacity (CEC) is
62cmol/kg。
6. the method for the drought-enduring cabbage type rape variety of screening according to claim 1, it is characterised in that:
Entire screening process carries out in intelligent greenhouse, and greenhouse air relative humidity remains 70%, and temperature is 20 DEG C.
7. the method for the drought-enduring cabbage type rape variety of screening according to claim 1, it is characterised in that:
In the step S1, a length of 100cm, width 50cm, the depth 30cm of the cultivation bed.
8. the method for the drought-enduring cabbage type rape variety of screening according to claim 1, it is characterised in that:
In the step S1, the cladding thickness of matrix is 1.5~2.5cm, preferably 2cm.
9. the method for the drought-enduring cabbage type rape variety of screening according to claim 1, it is characterised in that:
In the step S2, the Osmotic treatment group, 1 group of the control and 2 groups of the control respectively include three cultivation beds.
10. the method for the drought-enduring cabbage type rape variety of screening according to claim 1, it is characterised in that:
In the step S2, several days are 40 days.
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| CN113439624A (en) * | 2021-08-18 | 2021-09-28 | 湖南省作物研究所 | Method for screening cabbage type rape varieties suitable for mechanical harvesting and application thereof |
| CN113728887A (en) * | 2021-08-23 | 2021-12-03 | 河北省农林科学院旱作农业研究所 | Directional culture method of water-saving drought-resistant wheat |
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| CN110029190A (en) * | 2019-05-27 | 2019-07-19 | 西南大学 | A kind of cabbage type rape Drought-tolerant gene and its molecular labeling and application |
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| CN113439624B (en) * | 2021-08-18 | 2022-05-03 | 湖南省作物研究所 | Method for screening cabbage type rape varieties suitable for mechanical harvesting and application thereof |
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