CN106106137A - A kind of improve the anti-sclerotiniose of Brassica campestris L, the breeding method of lodging resistance ability - Google Patents
A kind of improve the anti-sclerotiniose of Brassica campestris L, the breeding method of lodging resistance ability Download PDFInfo
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- CN106106137A CN106106137A CN201610652500.2A CN201610652500A CN106106137A CN 106106137 A CN106106137 A CN 106106137A CN 201610652500 A CN201610652500 A CN 201610652500A CN 106106137 A CN106106137 A CN 106106137A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
Abstract
The invention discloses and a kind of improve the anti-sclerotiniose of Brassica campestris L, the breeding method of lodging resistance ability, including: with backbone parent town 8901, the derived material town 99S18 in town 8705 as female parent, hybridize with rape variety Zhejiang oil 18 for male parent；The continuous selfing of filial generation；From generation to generation, between each strain and height sense sclerotiniose strain, ratio plantation, mixes thoroughly previous year sclerotium and compound fertilizer, applies as base manure F0 F2；In florescence, pour water at field trench digging and keep hydrops and spray water；F3 proceeds by individual plant selection from generation to generation, and the individual plant that growth selection gesture is good, disease resistance is good carries out indoor species test and quality inspection, eliminates single pearl character and double inferior strain is reserved seed for planting.This method can be polymerized the excellent antibacterial core ospc gene of backbone parent and the stable high yield characteristic of large area production kind at short notice, the strain Comprehensive Traits of selection-breeding is good, and economical character is excellent, and anti-sclerotiniose and lodging resistance are strong, yield is high and steady, and method simplicity fast and reliable, purposiveness are strong.
Description
Technical field
The present invention relates to the breeding method of a kind of plant, improve the anti-sclerotiniose of Brassica campestris L, anti-fall more specifically to one
The breeding method of sexuality.
Background technology
Along with improving constantly of rapid development of economy and living standards of the people, market is to the demand of Semen Brassicae campestris increasingly
Greatly, world's oils and fats supply has tended to nervous, and China faces the problem that oil supply amount is not enough equally.Within 2009, Oleum Brassicae campestris accounts for
More than the 57% of domestic oil crop oil-producing, has become as domestic edible vegetable oil first and originates greatly.Meanwhile, China is still
Oil plant consumption big country of the world.2009 annual China vegetable oil total quantity consumeds reach 25,000,000 tons according to statistics.But domestic vegetable oil
Annual production maintains about 10,000,000 tons in recent years, and the degree of self-sufficiency is less than 40%.Wherein Oleum Brassicae campestris produces 4500000 tons per year, accounts for domestic planting
The 42.8% of thing oil total amount.Therefore Oleum Brassicae campestris has very important status in China edible oil market.China's oil in recent years
Material import volume is continuously increased, and vegetable oil material has become large agricultural product that China's external dependence degree is maximum the most.It was predicted that the year two thousand twenty
China year vegetable oil consumption figure 20kg per capita, consumption demand total amount is up to nearly 30,000,000 tons, is the 3 of Present Domestic production capacity
Times.It is contemplated that, in the following long term, China's vegetable oil production will present supply wretched insufficiency, inlet pressure doubles
Situation, producing the contradiction with demand will become increasingly conspicuous, " the oils and fats safety " of serious threat China.Therefore, Brassica campestris L is greatly developed raw
Producing, improving supply is to alleviate current imbalance between supply and demand, the Important Action of reduction importation dependence.
As the Brassica campestris L producing region of the Yangtze river basin accounting for the whole nation total cultivated area of Brassica campestris L nearly 70%, the time-consuming traditional nursery taken a lot of work
Transplanting method, often runs into cloudy weather for several days running weather in rape harvest season, and Brassica campestris L can be caused not gather in the crops in time, inefficiencies, oil
Farming season dish harvest season is tight, traditional artificial or harvesting program of semi-mechanized operation, including cutting-transport-shine the operations such as-Tuo-raise,
Labor intensity is big, production efficiency is low, and compared with Oryza sativa L., Semen Tritici aestivi, mechanization degree is the lowest, and peasant household and plantation family plantation Brassica campestris L are positive
Property decline.Along with Oryza sativa L., the raising of harvesting wheat machinery degree, Rape industry is the most urgent to the requirement of harvesting mechanization
Cut.
Rape production mechanization degree is the highest is the key factor restricting Brassica campestris L development.Lower Changjiang River region Economic contrast is sent out
Reaching, labour force is the most nervous, and the kind being suitable for high efficient oil colza implant model lacks.Sclerotiniose is that THE LOWER YANGTZE VALLEY Brassica campestris L is the most sick
Evil, rape variety disease resistance is the highest；And Brassica campestris L lodging resistance and disease resistance are the main of restriction Brassica campestris L entire mechanization operation
One of factor, mechanization degree is the highest causes rapeseed cultivation comparative effectiveness low, seriously hinders the development of Rape industry, therefore,
Screening is suitable for the rape variety of do mechanization operation, takes gently to simplify, mechanized farming, is the important measures of development Rape-seed production.
Therefore in Brassica campestris L selection-breeding theory, traditional " high yield, high-quality, disease-resistant " breeding objective it is updated to " high yield, high-quality, multi-resistance, suitable
Close mechanization " a whole new mind, using the character screening of applicable do mechanization operation as breeding normality, hard with stem stalk, lodging resistance is strong,
Anti-(resistance to) sclerotiniose, yield stability is good, and comprehensive agronomy character is suitable for high-quality resource that machine sowing machine receives as main body backbone parent, takes into account
Multiple ecotypes, the polymorphic differences of system, use conventional meanses such as backcrossing, reestablish diplomatic relations, merges beneficial gene, selects and is protecting
On the premise of card is suitable for do mechanization operation, effectively the multiple excellent character of polymerization, produces eurytopicity kind and the kind of super close effect
Popularization and application on producing, for grain and oil safety, growth of agricultural efficiency, increasing peasant income play an important role.
Summary of the invention
It is an object of the invention to solve the practice of current Rape-seed production is deposited in problems faced and existing conventional techniques
Defect, it is provided that a kind of improving the anti-sclerotiniose of Brassica campestris L, the breeding method of lodging resistance, the present invention is by following technical scheme
Realize:
A kind of improve the anti-sclerotiniose of Brassica campestris L, the breeding method of lodging resistance ability, including: with backbone parent town 8901, town
The derived material town 99S18 of 8705 is maternal, hybridizes with rape variety Zhejiang oil 18 for male parent；The continuous selfing of filial generation,
Filter out the rape variety of anti-sclerotiniose, lodging resistance.
In Juvenile stage of the present invention, backbone parent town 8901, the morning derivative progeny material town 99S18 ripe phase in town 8705, product
Measure anti-sclerotiniose high, high, lodging resistance is strong, plant type is compact, male parent Zhejiang oil 18 have good economical character, oil content height, high yield,
High-quality is double low, the female parent of above-mentioned kind and paternal hybrid, it is possible to cultivate the rape variety of anti-sclerotiniose, lodging resistance.
General continuous selfing 6～7 generation.
From generation to generation, in each generation screens, ratio plantation between each strain and height sense sclerotiniose strain, simultaneously by upper for F0-F2
Sclerotium and the compound fertilizer of the sclerotinia rot of colza that one year gathered mix thoroughly, apply as base manure；(generally 1-April of April in florescence
20 days), pour water at field trench digging and keep hydrops every day each aerosol apparatus sooner or later to carry out once water spray process, keep relative humidity
About 80% (generally 70%-90%) high humility.The present invention applies sclerotium, makes the sick garden that virulence is strong, (April 1 in florescence
Day-April 20) pour water holding hydrops carry out water spray process at field trench digging, keep relative humidity about 80% (70%-
90%), create the onset condition being conducive to sclerotiniose, than plantation simultaneously and between height sense kind, increase Disease Stress, be conducive to sieve
Select the strain that true and reliable disease-resistant performance is excellent.
The weight ratio of described sclerotium and compound fertilizer is 1:9-10.
Described high sense sclerotiniose strain is the napus lines of the high sense sclerotiniose filtered out in breeding process, the application
In the town 95S318 that relates to i.e. screen in selecting garden when breeding of the present invention and obtain.High sense sclerotiniose strain is in breeding department
Selecting to be easily screened for obtaining in garden, using high sense sclerotiniose strain purpose is to induce close kind sclerotiniose, and production is promoted
The general effect of rape variety utilized is bad.
F3 proceeds by individual plant selection from generation to generation, during selection-breeding, in florescence (generally April 20 April 1), opens in field
Ditch is poured water and is kept hydrops every day each aerosol apparatus sooner or later to carry out once water spray process, keeps relative humidity about 80% (general
For 70%-90%).The individual plant that growth selection gesture is good, disease resistance is good, resistant to lodging carries out indoor species test and quality inspection, eliminates single
Pearl character and double inferior strain are reserved seed for planting；The final stable strain selected is carried out multiple spot disease garden sclerotiniose identify.
Concrete, breeding method includes:
(1) F0 hybridization: with backbone parent town 8901, the derivative progeny material town 99S18 in town 8705 as female parent, with Brassica campestris L product
Plant Zhejiang oil 18 to hybridize for male parent, obtain F0 seed；
(2) F1 selfing: the sclerotium collected using the prior year and compound fertilizer mix thoroughly as base manure, by F0 seed and high sense sclerotiniose
Than plantation between strain, florescence, at field trench digging hydrops spraying water, improves humidity, growth selection advantage is strong, better resistance, agriculture
The individual plant that skill character is good carries out mixed receipts, obtains seed i.e. F2；
(3) F2 selfing: sclerotium and the compound fertilizer collected using the prior year mix thoroughly as base manure, by F2With height sense sclerotiniose strain
Between than plantation, only control worm not diseases prevention in field, florescence, at field trench digging hydrops spraying water, improves humidity, selects midwifery long
The individual plant that gesture is good, disease is light is reserved seed for planting after carrying out the eliminating of indoor species test and quality inspection, single pearl character and double low-quality performance difference
I.e. F3；
(4) F3 selfing: divide individual plant to sow the F3 reserved seed for planting, only controls worm not diseases prevention, and florescence goes forward side by side at field trench digging hydrops
Row water spray, bolting initial stage applying carbamide, selects the individual plant that midwifery growing way is good, disease is light, resistant to lodging to carry out indoor species test and quality inspection
Testing, reserve seed for planting after the eliminating of single pearl character and double low-quality performance difference i.e. F4；
(5) F4 selfing: divide individual plant to sow the F4 reserved seed for planting, only controls worm not diseases prevention, and florescence goes forward side by side at field trench digging hydrops
Row water spray, bolting initial stage applying carbamide, selects the individual plant that midwifery growing way is good, disease is light, resistant to lodging to carry out indoor species test and quality inspection
Testing, reserve seed for planting after the eliminating of single pearl character and double low-quality performance difference i.e. F5；
(6) F5 selfing: divide individual plant to sow the F5 reserved seed for planting, only controls worm not diseases prevention, and florescence goes forward side by side at field trench digging hydrops
Row water spray, bolting initial stage applying carbamide, selects the individual plant that midwifery growing way is good, disease is light, resistant to lodging to carry out indoor species test and quality inspection
Testing, reserve seed for planting after the eliminating of single pearl character and double low-quality performance difference i.e. F6；
(7) multiple spot disease garden sclerotiniose is identified: by F6Carry out Yield test and multispots trial complication garden inoculated identification, elected excellent
The napus lines that the double anti-sclerotiniose low, high of matter, stable high yield are anti-fall.
Compared with prior art, the invention have the benefit that
1, select with backbone parent town 8901, the derivative progeny material town 99S18 in town 8705 as female parent, stem stalk is hard, anti-
Falling property is strong, anti-(resistance to) sclerotiniose, and yield stability is good, and comprehensive agronomy character is suitable for machine sowing machine and receives, for the free strain in this research department.Select
Screening is introduced material, the excellent strain in centre and the commercial variety that currently large area produces and is handed over parent as hybridization, reply, can be in short-term
Between be polymerized the excellent character of each parent, make improved variety (being) resistance, yield is high and steady, and value is high；
The backbone parent related in the technology of the present invention method is our unit's self-fertile, has uniqueness and originality.
Town 8705: horse exerts × town 7517, town 7517 is this research department's selective nursery intermediate materials, and horse exerts introduction Canada resource
Yield is steady.High anti-sclerotiniose and virosis lodging resistance are strong.
Town 8901: town 7517 × town 7501) × CHR1617, town 7517,7501 is this research department's selective nursery intermediate materials,
CHR1617 introduces Canada's resource material.High anti-sclerotiniose and virosis, lodging resistance is strong, and yield is high.
Oil No. 10 // town 8705/, Hunan, 99S18:(town 8901/, town thuja oil 10), town 8901, town 8705 are research department's self-fertile
Backbone parent, Hunan oil 10 and thuja oil 10 are respectively quoted from area, the Hunan Huaihua institute of agricultural sciences and Jiangsu Province Agriculture Science Institute.
The present invention use innovation Disease Stress method (sclerotium and base manure applies simultaneously, between susceptible strain than plantation, later stage
Water spray moisturizing) make improved line antibacterial core characteristic of disease true and reliable stable.
In breeding process of the present invention, offspring stablizes seed and carries out multispots trial complication garden inoculated identification, it is ensured that resistance steady
Determine and broad spectrum activity.
Breeding method of the present invention advantageously reduces triviality and the blindness of work, and the procedure of breeding is shot the arrow at the target, and shortening is educated
Become the time of target strain；
Improved variety of the present invention (being) is suitable for do mechanization operation, is used in breeding and production, alleviates disease and occurs and lodging
The production loss caused, provides technical support for Brassica campestris L entire mechanization operation, promotes Rape industry competitiveness.
Breeding method of the present invention is easy easily to be grasped, and the most easily grasps in general breeding work.
Accompanying drawing explanation
Fig. 1 the technology of the present invention route schematic diagram.
Detailed description of the invention
Below in conjunction with specific embodiment, it is further elucidated with the present invention, it should be understood that these embodiments are merely to illustrate the present invention
Rather than restriction the scope of the present invention, after having read the present invention, the those skilled in the art's various equivalences to the present invention
The amendment of form all falls within the application claims limited range.
Embodiment 1
1) F0 hybridizes the selection of 2005 years free morning ripe phase, yield anti-sclerotiniose high, high, lodging resistance is strong, plant type is compact
Backbone parent town 8901, derivative progeny material Wild cabbage type normal rapeseed kind town 99S18 (Hunan, town 8901/ oil 10 in town 8705
Number // town 8705/ thuja oil 10) and make female parent, with introducing, there is good economical character, oil content height, high yield, the low Zhejiang of high-quality pair
Paternal hybrid made by oil 18, and hybridization obtains 66, F0 seed；
2) prior year is collected by F1 selfing sclerotium and compound fertilizer's ratio of 1:10 by weight are mixed thoroughly, every mu of 30 kilograms of compound fertilizer
(nitrogen: phosphorus: potassium=15:15:15) applies together as base manure.The F0 hybrid seed of results, each combination and high sense kind are (here
Select height sense sclerotiniose strain town 95S318, be breeding process to select garden is screened to obtain, it is also possible to for selecting in breeding
Strains of other high sense sclerotinioses of screening in journey) between than plantation, 2 row, florescence is at field trench digging hydrops and sprays water, and protects
Hold high humility.Selection nourishes and grows that advantage is strong, good (plant height about 170cm, plant type are compact, once for better resistance, economical character
Branch 8-10, base length about 35cm, about main axis length 70cm, spindle angular fruit number 80-100, the effective branch of Herb
Several more than 400, mass of 1000 kernel more than 4g) individual plant carry out mixed receipts, obtaining many of seed is F2。
3) sclerotium and the compound fertilizer that the prior year is collected by F2 selfing mixes thoroughly, applies together with base manure.By F2Sow 10 row, and
High sense kind (such as town 95S318) 2 ratio plantation in the ranks, only controls worm not diseases prevention in field, goes forward side by side at field trench digging hydrops in florescence
Row water spray, keeps high humility.Select individual plant 17 strain that midwifery growing way is good, disease is light, carry out indoor species test and quality inspection simultaneously, single
Eliminating of pearl character and double low-quality performance difference, 5 strains done very well are reserved seed for planting i.e. F3。
4) F3 selfing divides individual plant to sow the F3 reserved seed for planting, and only controls worm not diseases prevention, and florescence is at field trench digging hydrops and carries out
Water spray, keeps high humility, bolting initial stage i.e. heavy dressing carbamide 20kg/ mu, increases resistant to lodging selection and presses.Select that midwifery growing way is good, disease
Gently, individual plant 17 strain resistant to lodging, carry out washing in a pan of indoor species test and quality inspection, single pearl character and double low-quality performance difference simultaneously
Eliminating, 5 strains done very well are reserved seed for planting i.e. F4。
5) F4 selfing divides individual plant to sow the F4 reserved seed for planting, and only controls worm not diseases prevention, and florescence is at field trench digging hydrops and carries out
Water spray, keeps high humility.Bolting initial stage i.e. heavy dressing carbamide 20kg/ mu, increases resistant to lodging selection and presses.Select that midwifery growing way is good, disease
Gently, individual plant 21 strain resistant to lodging, carry out washing in a pan of indoor species test and quality inspection, single pearl character and double low-quality performance difference simultaneously
Eliminating, wherein numbering " 08S528 " behaves oneself best, and chooses 6 strains and reserves seed for planting i.e. F5.
6) F5 selfing divides individual plant to sow the F5 reserved seed for planting, and only controls worm not diseases prevention, and florescence is at field trench digging hydrops and carries out
Water spray, keeps high humility.Bolting initial stage i.e. heavy dressing carbamide 20kg/ mu, increases resistant to lodging selection and presses.Select that midwifery growing way is good, disease
Gently, resistant to lodging, select the individual plant that midwifery growing way is good, disease is light, carry out indoor species test and quality inspection simultaneously, individual plant character and double low
Eliminating of quality performance difference, wherein numbering " 10S329 " behaves oneself best, and strain Comprehensive Traits is excellent.
7) F6 selfing numbering " 10S329 " enters Yield test, multiple strain seeds is carried out multispots trial complication garden simultaneously
Inoculated identification, numbering " town 12F28 " yield height and stable yields, anti-sclerotiniose broad spectrum activity is good.
8) elected stable strain " town 12F28 " being carried out new lines variety comparative test, whole process not diseases prevention, " town 12F28 " is excellent
The disease-resistant anti-fall high yield of matter, i.e. obtains the double anti-sclerotiniose low, high of high-quality, the anti-fall conventional cabbage type rape strain " town of stable high yield
12F28 ", continue excellent middle to select the special excellent economical character of excellent holding " town 12F28 " and quality later.
9) " town 12F28 " participates in Jiangsu Province's high-quality normal rapeseed pilot study；
10) " town 12F28 " participates in Jiangsu Province's high-quality normal rapeseed regional testing；Concrete breeding is through being shown in Table 1.
Table 1 " town 12F28 " breeding is passed through
" town 12F28 " feature and cultural technigue that breeding method of the present invention is bred as are as follows:
Through planting experimentally, the yield forming that " town 12F28 " per mu yield is more than 200 kilograms: transplant 7500-9000 strain, strain for every mu
High about 170cm, primary branch number 9-10, Secondary branches number 4-6, the effective branch amount of Herb about 420, angular length
6.58cm, every seed number per pod about 22, mass of 1000 kernel is 4.35 grams.2014-2015 year sclerotiniose inoculated identification, 40 inoculations
In strain, " town 12F28 " anti-sclerotiniose ranks first place.Plant type is compact, and lodging resistance is strong, and mass of 1000 kernel is high, and single productive forces is high, is
The good kind of one applicable entire mechanization operation.
" town 12F28 " belongs to ripe double low cabbage type rapes in semi-winterness, and flower bud is big, Hua great, and angle fruit endures, individual plant effective angle fruit number
Many.Rational close planting, promotees early to send out the most enough Seedling intertillage in time and loosens the soil and eliminate, improve Population Quality, accomplish fluently high yield basic, fits guaranteeing
Preferably on the basis of Basic Seedling.Promote that angle fruit number is grown, thus reach angle fruit many and balance, purpose that grain is striven for high yield greatly, i.e. surely
On the basis of determining angle fruit number, increase grain number, improve grain weight, capture high yield.
That should grasp has technical point that
1, proper seeding North Jiangsu Area is preferably in JIUYUE about 5 days sowing, and Su Zhonghe riparian area is broadcast in JIUYUE for about 10 days
Kind, southern area of Jiangsu Province is in JIUYUE about 15 days sowing.The seedbed amount of broadcasting 0.5-0.6 kg/acre, accomplishes light seeding, even broadcasts, it is ensured that strong sprout moves
Plant.Timely thinning, about the 40 days length of time rice seedlings grow is wanted after emerging.
2, nurturing staff seedbed controls, at about 1:5-6, to use sufficient base fertilizer with land for growing field crops ratio, uses boron fertilizer and phosphorus potash fertilizer.
3, rational close planting transplanting time was advisable with late October, at the latest the first tenday period of a month in November.Transplanting Density is typically at 0.8-1.0
Ten thousand/mu, Fertilization Level is high, transplant field early can suitably reduce.Taking the transplanting method of the narrow strain of wide row, distance between rows and hills is 1.2 chis
× 5-6 cun.Live density is 1.2-1.3 ten thousand/mu, and after thinning final singling, distance between rows and hills is 1.2 chis × 4-5 cun.
4, rich water quality management every mu executes purity nitrogen 15-20 kilogram, heavy dressing base manure, the most early executes and carries seed manure and tongue fertilizer.Wherein base manure
Should account for the 60% of total dose, and ensure that N, P, K, B coordinate, early execute carrying seed manure, it is ensured that strong sprout survives the winter after transplanting alive, tongue is fertile preferably
The most early execute, typically rest at the beginning of late January or 2 months, use during tongue height 2-3 centimetre.
5, make soil list processing with Brassica campestris L herbicides special before disease pest and weed preventing and treating is transplanted, control the Brassica campestris L whole season of growth
Weed growth.Seedling stage keypoint control aphid and Pieris rapae.Result is planted experimentally in intermediate experiment and demonstration
Result of the test
Carrying out qualification test 2011-2012 year, average yield per mu 213.25 kilograms, than comparison Suyou 4 volume increase 7.17%；
Enter variety comparative test, average yield per mu 209.42 kilograms, ratio comparison Suyou 4 volume increase 6.28% 2012-2013 year, increase production the most aobvious
Write；The average yield per mu 157.42 kilograms in saving pilot study of 2013-2014 year, than comparison Suyou 4 volume increase 7.62%；
The average yield per mu 179.18 kilograms in saving regional testing of 2014-2015 year, than comparison Suyou 4 volume increase 2.14%.
Yield level
General per mu yield 180 kilograms, high yield field is up to more than 230 kilograms.2013 in 24.6 mu of Danyang, Jiangsu high yield side,
Per mu yield reaches 228.36 kilograms, the some high yield side, Wujiang of 2014 No. 7 (miscellaneous excellent) amounts of increase in production 2.12% more oily than the comparison Qin, 2005 years
This institute shows test, and per mu yield 211.32 kilograms, than comparison Suyou 4 volume increase 8.22%.
Resistance
" town 12F28 " all shows preferable resistance, 2014-2015 year sclerotium in intermediate experiment over the years to sclerotiniose
Sick inoculated identification, in the whole province 40 inoculation strain, " town 12F28 " anti-sclerotiniose ranks first place.Lodging resistance is extremely strong, does antiviral
Sick.
Claims (7)
1. one kind is improved the anti-sclerotiniose of Brassica campestris L, the breeding method of lodging resistance ability, it is characterised in that including: with backbone parent town
8901, the derived material town 99S18 in town 8705 is maternal, hybridizes with rape variety Zhejiang oil 18 for male parent；Filial generation is even
Continuous selfing, filters out the rape variety of anti-sclerotiniose, lodging resistance.
Breeding method the most according to claim 1, it is characterised in that selfing 6～7 generation continuously.
Breeding method the most according to claim 1, it is characterised in that F0-F2 is from generation to generation, in each generation screens, each
Than plantation between strain and height sense sclerotiniose strain, sclerotium and the compound fertilizer of the sclerotinia rot of colza of previous year collection are mixed simultaneously
Even, apply as base manure；In florescence, pour water at field trench digging and keep hydrops morning and evening every day each aerosol apparatus once to spray water
Process, keep relative humidity 70%-90%.
Breeding method the most according to claim 3, it is characterised in that the weight ratio of described sclerotium and compound fertilizer is 1:9-
10。
Breeding method the most according to claim 3, it is characterised in that described high sense sclerotiniose strain can be in breeding
During the napus lines of high sense sclerotiniose that filters out.
Breeding method the most according to claim 1, it is characterised in that F3 proceeds by individual plant selection from generation to generation, during selection-breeding, opens
At the florescence, pour water at field trench digging and keep hydrops every day each aerosol apparatus sooner or later to carry out once water spray process, keep relative humidity
For 70%-90%, the individual plant that growth selection gesture is good, disease resistance is good, resistant to lodging carries out indoor species test and quality inspection, eliminates single pearl
Character and double inferior strain are reserved seed for planting；The final stable strain selected is carried out multiple spot disease garden sclerotiniose identify.
Breeding method the most according to claim 1, it is characterised in that step includes:
(1) F0 hybridization: with backbone parent town 8901, the derivative progeny material town 99S18 in town 8705 as female parent, with rape variety Zhejiang
Oil 18 hybridizes for male parent, obtains F0 seed；
(2) F1 selfing: the sclerotium collected using the prior year and compound fertilizer mix thoroughly as base manure, by F0 seed and high sense sclerotiniose strain
Between than plantation, at field trench digging hydrops spraying water, improve humidity florescence, growth selection advantage is strong, better resistance, agronomy
The individual plant that shape is good carries out mixed receipts, obtains seed i.e. F2；
(3) F2 selfing: sclerotium and the compound fertilizer collected using the prior year mix thoroughly as base manure, by F2And compare between height sense sclerotiniose strain
Plantation, only controls worm not diseases prevention in field, and florescence, at field trench digging hydrops spraying water, improves humidity, select midwifery growing way good,
The individual plant that disease is light is reserved seed for planting after carrying out the eliminating of indoor species test and quality inspection, single pearl character and double low-quality performance difference i.e. F3；
(4) F3 selfing: divide individual plant to sow the F3 reserved seed for planting, only controls worm not diseases prevention, and florescence is at field trench digging hydrops and sprays
Water, the bolting initial stage applies carbamide, selects the individual plant that midwifery growing way is good, disease is light, resistant to lodging to carry out indoor species test and quality inspection, single
Reserve seed for planting after the eliminating of pearl character and double low-quality performance difference i.e. F4；
(5) F4 selfing: divide individual plant to sow the F4 reserved seed for planting, only controls worm not diseases prevention, and florescence is at field trench digging hydrops and sprays
Water, the bolting initial stage applies carbamide, selects the individual plant that midwifery growing way is good, disease is light, resistant to lodging to carry out indoor species test and quality inspection, single
Reserve seed for planting after the eliminating of pearl character and double low-quality performance difference i.e. F5；
(6) F5 selfing: divide individual plant to sow the F5 reserved seed for planting, only controls worm not diseases prevention, and florescence is at field trench digging hydrops and sprays
Water, the bolting initial stage applies carbamide, selects the individual plant that midwifery growing way is good, disease is light, resistant to lodging to carry out indoor species test and quality inspection, single
Reserve seed for planting after the eliminating of pearl character and double low-quality performance difference i.e. F6；
(7) multiple spot disease garden sclerotiniose is identified: by F6Carrying out Yield test and multispots trial complication garden inoculated identification, elected high-quality is double
The napus lines that anti-sclerotiniose low, high, stable high yield are anti-fall.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610652500.2A CN106106137B (en) | 2016-08-10 | 2016-08-10 | It is a kind of to improve the anti-sclerotiniose of rape, the breeding method of anti-fall sexuality |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201610652500.2A CN106106137B (en) | 2016-08-10 | 2016-08-10 | It is a kind of to improve the anti-sclerotiniose of rape, the breeding method of anti-fall sexuality |
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| CN106577266A (en) * | 2016-12-23 | 2017-04-26 | 滁州绿泉生态农业有限公司 | Breeding method for anti-aphid oilseed rape on basis of gene mapping technique |
| CN111149689A (en) * | 2020-03-09 | 2020-05-15 | 汉中市农业科学研究所（陕西省水稻研究所） | Breeding method of cabbage type hybrid rape with antibacterial nuclear disease |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101336611A (en) * | 2008-07-04 | 2009-01-07 | 江苏丘陵地区镇江农业科学研究所 | Breeding method of good quality, double-low, disease-resistant, high yield, lodging-resistant rape |
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| CN101336611A (en) * | 2008-07-04 | 2009-01-07 | 江苏丘陵地区镇江农业科学研究所 | Breeding method of good quality, double-low, disease-resistant, high yield, lodging-resistant rape |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106577266A (en) * | 2016-12-23 | 2017-04-26 | 滁州绿泉生态农业有限公司 | Breeding method for anti-aphid oilseed rape on basis of gene mapping technique |
| CN111149689A (en) * | 2020-03-09 | 2020-05-15 | 汉中市农业科学研究所（陕西省水稻研究所） | Breeding method of cabbage type hybrid rape with antibacterial nuclear disease |
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